TY  - JOUR
AU  - Glogovac, Svetlana
AU  - Gvozdanović-Varga, Jelica
AU  - Kiprovski, Biljana
AU  - Zorić, Miroslav
AU  - Nagl, Nevena
AU  - Brbaklić, Ljiljana
AU  - Trkulja, Dragana
PY  - 2024
UR  - http://fiver.ifvcns.rs/handle/123456789/4308
AB  - With aim to improve characterization and eventually, utilisation of garlic collection from  the Institute of Field and Vegetable Crops in Serbia, the genetic diversity of 52 accessions was estimated 
based on microsatellite markers, agronomic, biochemical and phenotypic traits (UPOV descriptor). 
The Shannon index H′ with a value of 0.47–0.95 indicated a high level of diversity for the greatest 
number of examined traits according to the UPOV descriptor. Based on agronomic and biochemical traits, groups of genotypes with the highest potential for yield and bioactive properties improvement were singled out. Molecular evaluation indicated the presence of duplicates in the collection and necessity of further research with additional number of DNA markers. The obtained results are important from the aspects of breeding and development of new varieties with improved yield and functional value as well as more economical conservation of genetic resources. Since diversity of plant genetic resources constantly decreases, the obtained results have long-term significance because preserved and genetically variable collections represent a valuable source of desirable genes for future human needs.
AB  - U cilju unapređenja karakterizacije i korišćenja uzoraka iz kolekcije belog luka Instituta za ratarstvo i povrtarstvo u Srbiji, genetički diverzitet 52 uzorka procenjen je na osnovu mikrosatelitskih markera, agronomskih, biohemijskih i fenotipskih osobina (UPOV deskriptor). Vrednosti Šenonovog indeksa H′ (0,47–0,95) ukazale su na visok nivo diverziteta za najveći broj ispitivanih osobina prema UPOV deskriptoru. Na osnovu agronomskih i biohemijskih osobina izdvojene su grupe genotipova sa najvećim potencijalom za poboljšanje prinosa i bioaktivnih svojstava. Molekularna evaluacija je ukazala na prisustvo duplikata u kolekciji i potrebu budućih istraživanja sa dodatnim brojem DNK markera. Dobijeni rezultati su značajni sa aspekta oplemenjivanja i razvoja novih sorti sa poboljšanim prinosom i funkcionalnom vrednošću, kao i ekonomičnijeg očuvanja genetičkih resursa. Dugoročno gledano, rezultati ukazuju na značaj očuvanja genetički varijabilnih kolekcija kao vrednog izvora poželjnih gena za buduće ljudske potrebe.
PB  - Springer Nature
T2  - Euphytica
T1  - Diversity of European genetic resources of garlic (Allium  sativum L.) from Serbian collection
IS  - 3
SP  - 37
VL  - 220
DO  - 10.1007/s10681-024-03292-8
ER  - 

@article{
author = "Glogovac, Svetlana and Gvozdanović-Varga, Jelica and Kiprovski, Biljana and Zorić, Miroslav and Nagl, Nevena and Brbaklić, Ljiljana and Trkulja, Dragana",
year = "2024",
abstract = "With aim to improve characterization and eventually, utilisation of garlic collection from  the Institute of Field and Vegetable Crops in Serbia, the genetic diversity of 52 accessions was estimated 
based on microsatellite markers, agronomic, biochemical and phenotypic traits (UPOV descriptor). 
The Shannon index H′ with a value of 0.47–0.95 indicated a high level of diversity for the greatest 
number of examined traits according to the UPOV descriptor. Based on agronomic and biochemical traits, groups of genotypes with the highest potential for yield and bioactive properties improvement were singled out. Molecular evaluation indicated the presence of duplicates in the collection and necessity of further research with additional number of DNA markers. The obtained results are important from the aspects of breeding and development of new varieties with improved yield and functional value as well as more economical conservation of genetic resources. Since diversity of plant genetic resources constantly decreases, the obtained results have long-term significance because preserved and genetically variable collections represent a valuable source of desirable genes for future human needs., U cilju unapređenja karakterizacije i korišćenja uzoraka iz kolekcije belog luka Instituta za ratarstvo i povrtarstvo u Srbiji, genetički diverzitet 52 uzorka procenjen je na osnovu mikrosatelitskih markera, agronomskih, biohemijskih i fenotipskih osobina (UPOV deskriptor). Vrednosti Šenonovog indeksa H′ (0,47–0,95) ukazale su na visok nivo diverziteta za najveći broj ispitivanih osobina prema UPOV deskriptoru. Na osnovu agronomskih i biohemijskih osobina izdvojene su grupe genotipova sa najvećim potencijalom za poboljšanje prinosa i bioaktivnih svojstava. Molekularna evaluacija je ukazala na prisustvo duplikata u kolekciji i potrebu budućih istraživanja sa dodatnim brojem DNK markera. Dobijeni rezultati su značajni sa aspekta oplemenjivanja i razvoja novih sorti sa poboljšanim prinosom i funkcionalnom vrednošću, kao i ekonomičnijeg očuvanja genetičkih resursa. Dugoročno gledano, rezultati ukazuju na značaj očuvanja genetički varijabilnih kolekcija kao vrednog izvora poželjnih gena za buduće ljudske potrebe.",
publisher = "Springer Nature",
journal = "Euphytica",
title = "Diversity of European genetic resources of garlic (Allium  sativum L.) from Serbian collection",
number = "3",
pages = "37",
volume = "220",
doi = "10.1007/s10681-024-03292-8"
}

Glogovac, S., Gvozdanović-Varga, J., Kiprovski, B., Zorić, M., Nagl, N., Brbaklić, L.,& Trkulja, D.. (2024). Diversity of European genetic resources of garlic (Allium  sativum L.) from Serbian collection. in Euphytica
Springer Nature., 220(3), 37.
https://doi.org/10.1007/s10681-024-03292-8

Glogovac S, Gvozdanović-Varga J, Kiprovski B, Zorić M, Nagl N, Brbaklić L, Trkulja D. Diversity of European genetic resources of garlic (Allium  sativum L.) from Serbian collection. in Euphytica. 2024;220(3):37.
doi:10.1007/s10681-024-03292-8 .

Glogovac, Svetlana, Gvozdanović-Varga, Jelica, Kiprovski, Biljana, Zorić, Miroslav, Nagl, Nevena, Brbaklić, Ljiljana, Trkulja, Dragana, "Diversity of European genetic resources of garlic (Allium  sativum L.) from Serbian collection" in Euphytica, 220, no. 3 (2024):37,
https://doi.org/10.1007/s10681-024-03292-8 . .

TY  - JOUR
AU  - Stankov Petreš, Aleksandra
AU  - Stojšin, Vera
AU  - Nagl, Nevena
AU  - Petreš, Mladen
AU  - Grahovac, Mila
AU  - Ćurčić, Živko
AU  - Budakov, Dragana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3344
AB  - Sugar beet is one of the most profitable crops in Serbia. The charcoal root rot is an emerging disease of sugar beet that significantly reduces yield and affects the economic viability of production. The objectives of this research were to determine the antagonist activity of selected Trichoderma harzianum isolates (T1 to T13) on two Macrophomina phaseolina isolates in vitro using a dual culture test and a volatile compounds test, as well as in vivo. The efficacy of these treatments was compared to two commercial biopesticides (Bacillomix® Original and Trifender Pro®) and one synthetic fungicide (Funomil 700 WG®). The strongest inhibition in the dual culture test was observed in the treatment with T. harzianum T2 against both tested M. phaseolina isolates. Also, the volatile compounds produced by isolates T2 and T12 exhibited the strongest inhibitory effect on M. phaseolina isolates (from 48.22 to 62.75%). Differences in efficacy were found not only between tested Trichoderma isolates, but also between M. phaseolina isolates used for inoculation which indicates different susceptibility of pathogen population to bioagent. The in vivo test confirmed the strong antagonistic effect of the T. harzianum T2 and T12 isolates. Bacillomix® Original, Trifender Pro® and Funomil® 700 WG did not express satisfactory effects in pathogen control.
PB  - Springer
T2  - Journal of Plant Diseases and Protection
T1  - Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet
DO  - 10.1007/s41348-023-00716-w
ER  - 

@article{
author = "Stankov Petreš, Aleksandra and Stojšin, Vera and Nagl, Nevena and Petreš, Mladen and Grahovac, Mila and Ćurčić, Živko and Budakov, Dragana",
year = "2023",
abstract = "Sugar beet is one of the most profitable crops in Serbia. The charcoal root rot is an emerging disease of sugar beet that significantly reduces yield and affects the economic viability of production. The objectives of this research were to determine the antagonist activity of selected Trichoderma harzianum isolates (T1 to T13) on two Macrophomina phaseolina isolates in vitro using a dual culture test and a volatile compounds test, as well as in vivo. The efficacy of these treatments was compared to two commercial biopesticides (Bacillomix® Original and Trifender Pro®) and one synthetic fungicide (Funomil 700 WG®). The strongest inhibition in the dual culture test was observed in the treatment with T. harzianum T2 against both tested M. phaseolina isolates. Also, the volatile compounds produced by isolates T2 and T12 exhibited the strongest inhibitory effect on M. phaseolina isolates (from 48.22 to 62.75%). Differences in efficacy were found not only between tested Trichoderma isolates, but also between M. phaseolina isolates used for inoculation which indicates different susceptibility of pathogen population to bioagent. The in vivo test confirmed the strong antagonistic effect of the T. harzianum T2 and T12 isolates. Bacillomix® Original, Trifender Pro® and Funomil® 700 WG did not express satisfactory effects in pathogen control.",
publisher = "Springer",
journal = "Journal of Plant Diseases and Protection",
title = "Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet",
doi = "10.1007/s41348-023-00716-w"
}

Stankov Petreš, A., Stojšin, V., Nagl, N., Petreš, M., Grahovac, M., Ćurčić, Ž.,& Budakov, D.. (2023). Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet. in Journal of Plant Diseases and Protection
Springer..
https://doi.org/10.1007/s41348-023-00716-w

Stankov Petreš A, Stojšin V, Nagl N, Petreš M, Grahovac M, Ćurčić Ž, Budakov D. Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet. in Journal of Plant Diseases and Protection. 2023;.
doi:10.1007/s41348-023-00716-w .

Stankov Petreš, Aleksandra, Stojšin, Vera, Nagl, Nevena, Petreš, Mladen, Grahovac, Mila, Ćurčić, Živko, Budakov, Dragana, "Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet" in Journal of Plant Diseases and Protection (2023),
https://doi.org/10.1007/s41348-023-00716-w . .

TY  - JOUR
AU  - Umićević, Sonja
AU  - Antić, Marina
AU  - Rajković, Dragana
AU  - Sinkovič, Lovro
AU  - Nagl, Nevena
AU  - Goreta Ban, Smiljana
AU  - Meglič, Vladimir
AU  - Todorović, Vida
AU  - Marjanović-Jeromela, Ana
AU  - Pipan, Barbara
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3784
AB  - Leafy kale (Brassica oleracea var. acephala L.) is one of the most
important Brassicaceae species with health-promoting properties and great
diversity. To investigate variability within and among accessions, 12 accessions
(each accession comprised four individuals/genotypes) from the Balkan region
were genotyped at 12 simple sequence repeats (SSRs) loci. The selected SSR
markers originated from the genomes of B. oleracea (eight), B. rapa (two) and
B. napus (two). DNA was extracted from seeds, cotyledons, shoots, and young
leaves, depending on the germination energy of each seed, using magnetic
extraction method. Genetic analysis included eight accessions from Serbia (24
genotypes), two accessions from Montenegro (eight genotypes), and one
accession from Bosnia and Herzegovina and Croatia (with four genotypes each).
Accession-specific polymorphism was found at the Ol12-FO2 and Ol10-F11a
loci, where genotypes were qualitatively distinguished by horizontal agarose gel
electrophoresis (binary detection). Among 12 loci, the average polymorphic
information content (PIC) was 0.71, with the marker Ol13-C12 proving to be the
78 Umićević et al.
most informative (PIC = 0.89). However, the analysis of molecular variability
(AMOVA) showed 11% diversity between accessions, 19% diversity between
genotypes, and 70% diversity within genotypes, explaining the molecular
diversity of native Balkan leafy kale germplasm. According to the genetic
structure, four genetic groups were formed with an average expected
heterozygosity of 0.70 between clusters. In order to exploit the genetic diversity,
it would be advisable to evaluate these accessions at phenotypic level and use
their potential in breeding programmes.
AB  - Raštan (Brassica oleracea var. acephala L.) je jedna od najvažnijih vrsta iz porodice kupusnjača koja povoljno utiče na zdravlje ljudi i odlikuje se velikom raznovrsnošću. Da bi se istražila varijabilnost unutar i između prinova, 12 prinova (svaka prinova se sastojala od četiri individue/genotipa) sa teritorije Balkanskog poluostrva je genotipizovano koristeći 12 SSR markera. Odabrani SSR markeri potiču iz genoma B. oleracea (osam), B. rapa (dva) i B. napus (dva). DNK je izolovana iz sjemena, kotiledona, izdanaka i mladih listova, u zavisnosti od energije klijanja svakog sjemena, metodom magnetne ekstrakcije. Genetičkom analizom obuhvaćeno je osam prinova iz Srbije (24 genotipa), dvije prinove iz Crne Gore (osam genotipova) i jedna prinova iz Bosne i Hercegovine i Hrvatske (sa po četiri genotipa). Polimorfizam specifičan za prinove pronađen je na lokusima Ol12-FO2 i Ol10-F11a, gdje su genotipovi kvalitativno razlikovani horizontalnom elektroforezom u agaroznom gelu (binarna detekcija). Kod ukupno 12 lokusa, prosječan sadržaj polimorfne informacije (PIC) bio je 0,71, pri čemu se kao najinformativniji pokazao marker Ol13-C12 (PIC = 0,89). Međutim, analiza molekularne varijabilnosti (AMOVA) pokazala je 11% raznovrsnosti između prinova, 19% raznovrsnosti između genotipova i 70% raznovrsnosti unutar genotipova, objašnjavajući molekularnu raznovrsnost izvorne germplazme raštana sa teritorije Balkanskog poluostrva. Prema genetičkoj strukturi formirane su četiri genetičke grupe sa prosječnom očekivanom heterozigotnošću od 0,70 između klastera. Da bi se iskoristio genetički diverzitet, bilo bi preporučljivo da se ove prinove karakterišu 90 Umićević et al. na fenotipskom nivou i da se njihov potencijal iskoristi u programima oplemenjivanja.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
T2  - Agroznanje / Agro-knowledge Journal
T1  - Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.)
T1  - Genetički diverzitet genetičkih resursa raštana (Brassica oleracea var. acephala L.)
EP  - 90
IS  - 3
SP  - 77
VL  - 24
DO  - 10.7251/AGREN2303077U
ER  - 

@article{
author = "Umićević, Sonja and Antić, Marina and Rajković, Dragana and Sinkovič, Lovro and Nagl, Nevena and Goreta Ban, Smiljana and Meglič, Vladimir and Todorović, Vida and Marjanović-Jeromela, Ana and Pipan, Barbara",
year = "2023",
abstract = "Leafy kale (Brassica oleracea var. acephala L.) is one of the most
important Brassicaceae species with health-promoting properties and great
diversity. To investigate variability within and among accessions, 12 accessions
(each accession comprised four individuals/genotypes) from the Balkan region
were genotyped at 12 simple sequence repeats (SSRs) loci. The selected SSR
markers originated from the genomes of B. oleracea (eight), B. rapa (two) and
B. napus (two). DNA was extracted from seeds, cotyledons, shoots, and young
leaves, depending on the germination energy of each seed, using magnetic
extraction method. Genetic analysis included eight accessions from Serbia (24
genotypes), two accessions from Montenegro (eight genotypes), and one
accession from Bosnia and Herzegovina and Croatia (with four genotypes each).
Accession-specific polymorphism was found at the Ol12-FO2 and Ol10-F11a
loci, where genotypes were qualitatively distinguished by horizontal agarose gel
electrophoresis (binary detection). Among 12 loci, the average polymorphic
information content (PIC) was 0.71, with the marker Ol13-C12 proving to be the
78 Umićević et al.
most informative (PIC = 0.89). However, the analysis of molecular variability
(AMOVA) showed 11% diversity between accessions, 19% diversity between
genotypes, and 70% diversity within genotypes, explaining the molecular
diversity of native Balkan leafy kale germplasm. According to the genetic
structure, four genetic groups were formed with an average expected
heterozygosity of 0.70 between clusters. In order to exploit the genetic diversity,
it would be advisable to evaluate these accessions at phenotypic level and use
their potential in breeding programmes., Raštan (Brassica oleracea var. acephala L.) je jedna od najvažnijih vrsta iz porodice kupusnjača koja povoljno utiče na zdravlje ljudi i odlikuje se velikom raznovrsnošću. Da bi se istražila varijabilnost unutar i između prinova, 12 prinova (svaka prinova se sastojala od četiri individue/genotipa) sa teritorije Balkanskog poluostrva je genotipizovano koristeći 12 SSR markera. Odabrani SSR markeri potiču iz genoma B. oleracea (osam), B. rapa (dva) i B. napus (dva). DNK je izolovana iz sjemena, kotiledona, izdanaka i mladih listova, u zavisnosti od energije klijanja svakog sjemena, metodom magnetne ekstrakcije. Genetičkom analizom obuhvaćeno je osam prinova iz Srbije (24 genotipa), dvije prinove iz Crne Gore (osam genotipova) i jedna prinova iz Bosne i Hercegovine i Hrvatske (sa po četiri genotipa). Polimorfizam specifičan za prinove pronađen je na lokusima Ol12-FO2 i Ol10-F11a, gdje su genotipovi kvalitativno razlikovani horizontalnom elektroforezom u agaroznom gelu (binarna detekcija). Kod ukupno 12 lokusa, prosječan sadržaj polimorfne informacije (PIC) bio je 0,71, pri čemu se kao najinformativniji pokazao marker Ol13-C12 (PIC = 0,89). Međutim, analiza molekularne varijabilnosti (AMOVA) pokazala je 11% raznovrsnosti između prinova, 19% raznovrsnosti između genotipova i 70% raznovrsnosti unutar genotipova, objašnjavajući molekularnu raznovrsnost izvorne germplazme raštana sa teritorije Balkanskog poluostrva. Prema genetičkoj strukturi formirane su četiri genetičke grupe sa prosječnom očekivanom heterozigotnošću od 0,70 između klastera. Da bi se iskoristio genetički diverzitet, bilo bi preporučljivo da se ove prinove karakterišu 90 Umićević et al. na fenotipskom nivou i da se njihov potencijal iskoristi u programima oplemenjivanja.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Agroznanje / Agro-knowledge Journal",
title = "Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.), Genetički diverzitet genetičkih resursa raštana (Brassica oleracea var. acephala L.)",
pages = "90-77",
number = "3",
volume = "24",
doi = "10.7251/AGREN2303077U"
}

Umićević, S., Antić, M., Rajković, D., Sinkovič, L., Nagl, N., Goreta Ban, S., Meglič, V., Todorović, V., Marjanović-Jeromela, A.,& Pipan, B.. (2023). Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Agroznanje / Agro-knowledge Journal
Banja Luka : University of Banja Luka, Faculty of Agriculture., 24(3), 77-90.
https://doi.org/10.7251/AGREN2303077U

Umićević S, Antić M, Rajković D, Sinkovič L, Nagl N, Goreta Ban S, Meglič V, Todorović V, Marjanović-Jeromela A, Pipan B. Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Agroznanje / Agro-knowledge Journal. 2023;24(3):77-90.
doi:10.7251/AGREN2303077U .

Umićević, Sonja, Antić, Marina, Rajković, Dragana, Sinkovič, Lovro, Nagl, Nevena, Goreta Ban, Smiljana, Meglič, Vladimir, Todorović, Vida, Marjanović-Jeromela, Ana, Pipan, Barbara, "Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.)" in Agroznanje / Agro-knowledge Journal, 24, no. 3 (2023):77-90,
https://doi.org/10.7251/AGREN2303077U . .

TY  - JOUR
AU  - Nagl, Nevena
AU  - Sinkovič, Lovro
AU  - Savić, Aleksandra
AU  - Isakov, Milada
AU  - Hasanaklou, Hourieh Tavakoli
AU  - Pipan, Barbara
AU  - Marjanović-Jeromela, Ana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3788
AB  - Grass pea (Lathyrus sativus) is a valuable grain legume known for its high protein content and rich essential amino acid profile. Its exceptional characteristics such as drought tolerance, high adaptability to extreme conditions, disease resistance, and low cultivation inputs, make it particularly suitable for cultivation by resource-poor farmers. However, the potential use of grass pea is limited due to the presence of anti-nutritional factors, including protease inhibitors, especially trypsin inhibitor. This study aimed to develop a rapid and reliable method for measuring TI activity in seeds of grass pea and to investigate the influence of genotype and environment on trypsin inhibitor (TI) activity in seeds of grass pea. A set of 25 grass pea accessions from seven European countries was cultivated in Slovenia at the Agricultural Institute of Slovenia, and a set of 12 grass pea accessions from the Serbian gene bank was grown in Serbia at the Institute of Field and Vegetable Crops. The TI activity varied considerably among the grass pea accessions studied, with values ranging from 26.7 to 90.3 TUI/mg. To further evaluate the effects of environmental conditions on TI activity, eight grass pea accessions originating from Serbia were grown in both Slovenia and Serbia. The TI activity of the accessions grown in Slovenia ranged from 26.7 to 81.0 TUI/mg, while the activity of the accessions grown in Serbia ranged from 40.3 to 57.0 TUI/mg. The correlation of TI activity between grass pea accessions grown in Slovenia and those grown in Serbia was 0.39, with genotype diversity being the largest contributor (55.9%). This study provides a valuable insight into the variability of TI activity in grass pea and shows the possible influence of environmental conditions on this trait. However, since the data are only from a one-year field trial, further studies are needed to fully evaluate the influence of different environmental factors on TI activity.
PB  - Novi Sad : Institute of Field and Vegetable Crops
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)
EP  - 39
IS  - 2
SP  - 32
VL  - 60
DO  - 10.5937/ratpov60-45934
ER  - 

@article{
author = "Nagl, Nevena and Sinkovič, Lovro and Savić, Aleksandra and Isakov, Milada and Hasanaklou, Hourieh Tavakoli and Pipan, Barbara and Marjanović-Jeromela, Ana",
year = "2023",
abstract = "Grass pea (Lathyrus sativus) is a valuable grain legume known for its high protein content and rich essential amino acid profile. Its exceptional characteristics such as drought tolerance, high adaptability to extreme conditions, disease resistance, and low cultivation inputs, make it particularly suitable for cultivation by resource-poor farmers. However, the potential use of grass pea is limited due to the presence of anti-nutritional factors, including protease inhibitors, especially trypsin inhibitor. This study aimed to develop a rapid and reliable method for measuring TI activity in seeds of grass pea and to investigate the influence of genotype and environment on trypsin inhibitor (TI) activity in seeds of grass pea. A set of 25 grass pea accessions from seven European countries was cultivated in Slovenia at the Agricultural Institute of Slovenia, and a set of 12 grass pea accessions from the Serbian gene bank was grown in Serbia at the Institute of Field and Vegetable Crops. The TI activity varied considerably among the grass pea accessions studied, with values ranging from 26.7 to 90.3 TUI/mg. To further evaluate the effects of environmental conditions on TI activity, eight grass pea accessions originating from Serbia were grown in both Slovenia and Serbia. The TI activity of the accessions grown in Slovenia ranged from 26.7 to 81.0 TUI/mg, while the activity of the accessions grown in Serbia ranged from 40.3 to 57.0 TUI/mg. The correlation of TI activity between grass pea accessions grown in Slovenia and those grown in Serbia was 0.39, with genotype diversity being the largest contributor (55.9%). This study provides a valuable insight into the variability of TI activity in grass pea and shows the possible influence of environmental conditions on this trait. However, since the data are only from a one-year field trial, further studies are needed to fully evaluate the influence of different environmental factors on TI activity.",
publisher = "Novi Sad : Institute of Field and Vegetable Crops",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)",
pages = "39-32",
number = "2",
volume = "60",
doi = "10.5937/ratpov60-45934"
}

Nagl, N., Sinkovič, L., Savić, A., Isakov, M., Hasanaklou, H. T., Pipan, B.,& Marjanović-Jeromela, A.. (2023). Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.). in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Novi Sad : Institute of Field and Vegetable Crops., 60(2), 32-39.
https://doi.org/10.5937/ratpov60-45934

Nagl N, Sinkovič L, Savić A, Isakov M, Hasanaklou HT, Pipan B, Marjanović-Jeromela A. Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.). in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2023;60(2):32-39.
doi:10.5937/ratpov60-45934 .

Nagl, Nevena, Sinkovič, Lovro, Savić, Aleksandra, Isakov, Milada, Hasanaklou, Hourieh Tavakoli, Pipan, Barbara, Marjanović-Jeromela, Ana, "Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 60, no. 2 (2023):32-39,
https://doi.org/10.5937/ratpov60-45934 . .

TY  - CONF
AU  - Umićević, Sonja
AU  - Antić, Marina
AU  - Rajković, Dragana
AU  - Sinkovič, Lovro
AU  - Nagl, Nevena
AU  - Goreta Ban, Smiljana
AU  - Meglič, Vladimir
AU  - Todorović, Vida
AU  - Marjanović-Jeromela, Ana
AU  - Pipan, Barbara
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4260
AB  - Leafy kale (Brassica oleracea var. acephala L.) is one of the most important Brassicaceae species with health-promoting properties and high diversity. To investigate variability within and between accessions, 12 accessions (each accession comprised four individuals/genotypes) from the Balkan area was genotyped at 12 SSR (Simple Sequence Repeats) loci. DNA was extracted from seeds, cotyledons, shoots, or young leaves, depending on the germination energy of each seed, using the MagMax (Applied Biosystems) magnetic extraction method with the BioSprint Plant Kit (Qiagen).
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina
T1  - Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.)
EP  - 189
SP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4260
ER  - 

@conference{
author = "Umićević, Sonja and Antić, Marina and Rajković, Dragana and Sinkovič, Lovro and Nagl, Nevena and Goreta Ban, Smiljana and Meglič, Vladimir and Todorović, Vida and Marjanović-Jeromela, Ana and Pipan, Barbara",
year = "2023",
abstract = "Leafy kale (Brassica oleracea var. acephala L.) is one of the most important Brassicaceae species with health-promoting properties and high diversity. To investigate variability within and between accessions, 12 accessions (each accession comprised four individuals/genotypes) from the Balkan area was genotyped at 12 SSR (Simple Sequence Repeats) loci. DNA was extracted from seeds, cotyledons, shoots, or young leaves, depending on the germination energy of each seed, using the MagMax (Applied Biosystems) magnetic extraction method with the BioSprint Plant Kit (Qiagen).",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina",
title = "Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.)",
pages = "189-188",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4260"
}

Umićević, S., Antić, M., Rajković, D., Sinkovič, L., Nagl, N., Goreta Ban, S., Meglič, V., Todorović, V., Marjanović-Jeromela, A.,& Pipan, B.. (2023). Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 188-189.
https://hdl.handle.net/21.15107/rcub_fiver_4260

Umićević S, Antić M, Rajković D, Sinkovič L, Nagl N, Goreta Ban S, Meglič V, Todorović V, Marjanović-Jeromela A, Pipan B. Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina. 2023;:188-189.
https://hdl.handle.net/21.15107/rcub_fiver_4260 .

Umićević, Sonja, Antić, Marina, Rajković, Dragana, Sinkovič, Lovro, Nagl, Nevena, Goreta Ban, Smiljana, Meglič, Vladimir, Todorović, Vida, Marjanović-Jeromela, Ana, Pipan, Barbara, "Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.)" in Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina (2023):188-189,
https://hdl.handle.net/21.15107/rcub_fiver_4260 .

TY  - JOUR
AU  - Glogovac, Svetlana
AU  - Takač, Adam
AU  - Belović, Miona
AU  - Gvozdanović-Varga, Jelica
AU  - Nagl, Nevena
AU  - Červenski, Janko
AU  - Danojević, Dario
AU  - Trkulja, Dragana
AU  - Prodanović, Slaven
AU  - Živanović, Tomislav
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2817
AB  - Tomato is one of the most important species belonging to the Solanaceae family. Focusing on the importance of tomato in human nutrition and the problem of narrowed genetic variability, the aim of the study was to assess morphological and chemical diversity in IFVCNS germplasm collection. Twenty genotypes were analysed for the morphological and chemical fruit traits: average mass (g), length (cm), diameter (cm), pericarp thickness (mm), locules number, moisture content (%), total soluble solids (°Brix), ash content (%), total acidity (%) and pH value. Selected plant material for analysis included: landraces, traditional varieties, breeding lines and commercial varieties. Differences among tomato genotypes in all fruit traits were determined. Fruit mass and locules number had the highest coefficient of variation. The least differences between genotypes were observed in the fruit moisture content. Four principal components accounted for 90.6% of total variance or 36.5%, 24.2%, 19.8% and 10.1%, respectively. Along the axis of the first main component, genotypes were classified into three groups. The first component was defined by fruit length, diameter and mass. The second component was correlated with pericarp thickness and locules number, and the third with moisture content, ash content and total soluble solids. Based on the cluster analysis, genotypes were classified into three groups which were in agreement with the PCA groups. Hybridization between genotypes from different groups was proposed in order to create new hybrids and varieties and to increase tomato germplasm diversity. By crossing those genotypes, improved recombinations in morphological and chemical traits can be expected.
AB  - Paradajz je jedna od najznačajnih vrsta iz familije Solanaceae. Fokusirajući se na značaj paradajza u ljudskoj ishrani i problem njegove sužene genetske varijabilnosti, cilj istraživanja je bio da se proceni morfološki i hemijski diverzitet u kolekciji germplazme Instituta za ratarstvo i povrtarstvo u Novom Sadu. Dvadeset genotipova je odabrano za analizu sledećih osobina ploda: prosečna masa (g), dužina (cm), širina (cm), debljina perikarpa (mm), broj komora, sadržaj vode (%), ukupna rastvorljiva suva materija (°Brix), sadržaj pepela (%), ukupna kiselost (%) i pH vrednost. Biljni materijal odabran za analizu obuhvatao je: lokalne populacije, stare sorte, oplemenjivačke linije i komercijalne sorte. Utvrđene su razlike između genotipova u svim ispitivanim osobinama. Prosečna masa ploda i broj komora imali su najveći koeficijent varijacije. Ispitivani genotipovi su se najmanje razlikovali u sadržaju vode u plodu. Četiri glavne komponente objasnile su 90,6% ukupne varijabilnosti ili 36,5%, 24,2%, 19,8% i 10,1%, redom. Duž ose prve glavne komponente genotipovi su klasifikovani u tri grupe. Prva komponenta je definisana dužinom, širinom i prosečnom masom ploda. Druga komponenta je bila u korelaciji sa debljinom perikarpa i brojem komora, a treća sa sadržajem vode, pepela i ukupne rastvorljive suve materije. Klaster analizom genotipovi su klasifikovani u tri grupe koje su bile u saglasnosti sa PCA grupama. U cilju stvaranja novih sorti i hibrida, kao i povećanja diverziteta germplazme paradajza, predložena je hibridizacija genotipova iz različitih grupa. Ukrštanjem tih genotipova mogu se očekivati unapređene rekombinacije u morfološkim i hemijskim osobinama.
PB  - Novi Sad : Institute of Field and Vegetable Crops
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Characterization of tomato genetic resources in the function of breeding
T1  - Karakterizacija genetičkih resursa paradajza u funkciji oplemenjivanja
EP  - 8
IS  - 1
SP  - 1
VL  - 59
DO  - 10.5937/ratpov59-36776
ER  - 

@article{
author = "Glogovac, Svetlana and Takač, Adam and Belović, Miona and Gvozdanović-Varga, Jelica and Nagl, Nevena and Červenski, Janko and Danojević, Dario and Trkulja, Dragana and Prodanović, Slaven and Živanović, Tomislav",
year = "2022",
abstract = "Tomato is one of the most important species belonging to the Solanaceae family. Focusing on the importance of tomato in human nutrition and the problem of narrowed genetic variability, the aim of the study was to assess morphological and chemical diversity in IFVCNS germplasm collection. Twenty genotypes were analysed for the morphological and chemical fruit traits: average mass (g), length (cm), diameter (cm), pericarp thickness (mm), locules number, moisture content (%), total soluble solids (°Brix), ash content (%), total acidity (%) and pH value. Selected plant material for analysis included: landraces, traditional varieties, breeding lines and commercial varieties. Differences among tomato genotypes in all fruit traits were determined. Fruit mass and locules number had the highest coefficient of variation. The least differences between genotypes were observed in the fruit moisture content. Four principal components accounted for 90.6% of total variance or 36.5%, 24.2%, 19.8% and 10.1%, respectively. Along the axis of the first main component, genotypes were classified into three groups. The first component was defined by fruit length, diameter and mass. The second component was correlated with pericarp thickness and locules number, and the third with moisture content, ash content and total soluble solids. Based on the cluster analysis, genotypes were classified into three groups which were in agreement with the PCA groups. Hybridization between genotypes from different groups was proposed in order to create new hybrids and varieties and to increase tomato germplasm diversity. By crossing those genotypes, improved recombinations in morphological and chemical traits can be expected., Paradajz je jedna od najznačajnih vrsta iz familije Solanaceae. Fokusirajući se na značaj paradajza u ljudskoj ishrani i problem njegove sužene genetske varijabilnosti, cilj istraživanja je bio da se proceni morfološki i hemijski diverzitet u kolekciji germplazme Instituta za ratarstvo i povrtarstvo u Novom Sadu. Dvadeset genotipova je odabrano za analizu sledećih osobina ploda: prosečna masa (g), dužina (cm), širina (cm), debljina perikarpa (mm), broj komora, sadržaj vode (%), ukupna rastvorljiva suva materija (°Brix), sadržaj pepela (%), ukupna kiselost (%) i pH vrednost. Biljni materijal odabran za analizu obuhvatao je: lokalne populacije, stare sorte, oplemenjivačke linije i komercijalne sorte. Utvrđene su razlike između genotipova u svim ispitivanim osobinama. Prosečna masa ploda i broj komora imali su najveći koeficijent varijacije. Ispitivani genotipovi su se najmanje razlikovali u sadržaju vode u plodu. Četiri glavne komponente objasnile su 90,6% ukupne varijabilnosti ili 36,5%, 24,2%, 19,8% i 10,1%, redom. Duž ose prve glavne komponente genotipovi su klasifikovani u tri grupe. Prva komponenta je definisana dužinom, širinom i prosečnom masom ploda. Druga komponenta je bila u korelaciji sa debljinom perikarpa i brojem komora, a treća sa sadržajem vode, pepela i ukupne rastvorljive suve materije. Klaster analizom genotipovi su klasifikovani u tri grupe koje su bile u saglasnosti sa PCA grupama. U cilju stvaranja novih sorti i hibrida, kao i povećanja diverziteta germplazme paradajza, predložena je hibridizacija genotipova iz različitih grupa. Ukrštanjem tih genotipova mogu se očekivati unapređene rekombinacije u morfološkim i hemijskim osobinama.",
publisher = "Novi Sad : Institute of Field and Vegetable Crops",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Characterization of tomato genetic resources in the function of breeding, Karakterizacija genetičkih resursa paradajza u funkciji oplemenjivanja",
pages = "8-1",
number = "1",
volume = "59",
doi = "10.5937/ratpov59-36776"
}

Glogovac, S., Takač, A., Belović, M., Gvozdanović-Varga, J., Nagl, N., Červenski, J., Danojević, D., Trkulja, D., Prodanović, S.,& Živanović, T.. (2022). Characterization of tomato genetic resources in the function of breeding. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Novi Sad : Institute of Field and Vegetable Crops., 59(1), 1-8.
https://doi.org/10.5937/ratpov59-36776

Glogovac S, Takač A, Belović M, Gvozdanović-Varga J, Nagl N, Červenski J, Danojević D, Trkulja D, Prodanović S, Živanović T. Characterization of tomato genetic resources in the function of breeding. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2022;59(1):1-8.
doi:10.5937/ratpov59-36776 .

Glogovac, Svetlana, Takač, Adam, Belović, Miona, Gvozdanović-Varga, Jelica, Nagl, Nevena, Červenski, Janko, Danojević, Dario, Trkulja, Dragana, Prodanović, Slaven, Živanović, Tomislav, "Characterization of tomato genetic resources in the function of breeding" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 59, no. 1 (2022):1-8,
https://doi.org/10.5937/ratpov59-36776 . .

TY  - JOUR
AU  - Živanov, Dalibor
AU  - Tančić-Živanov, Sonja
AU  - Savić, Aleksandra
AU  - Uhlarik, Ana
AU  - Miladinov, Zlatica
AU  - Medić-Pap, Slađana
AU  - Nagl, Nevena
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2678
AB  - Chickpea (Cicer arietinum L.) is one of the most commonly consumed legume crops worldwide, cultivated in more than 55 countries (FAO 2017). However, in Serbia it is a novel crop grown on approximately 120 acres, but the area under this crop slightly increases each year. Fusarium wilt caused by F. oxysporum f. sp. ciceris is one of the most economically important diseases in most chickpea-growing areas, but there has been no formal report of the Fusarium wilt of chickpea in Serbia. In June 2018, the first symptoms of Fusarium wilt were registered at Rimski Sancevi (Vojvodina Province), Serbia (N 45'19.3119, E 19'49.9339), as wilted chickpea plants grouped in patches on approximately 5% of plants in a 3-acre area. Symptoms of yellowing and necrosis of foliage appeared as late wilt in the podding stage. Roots of affected plants showed no external discoloration, but a cross-section showed dark-brown discoloration of xylem tissue. To isolate the causal agent, symptomatic plants were collected and 10 cuttings of root symptomatic tissue were surface disinfected with 2% sodium hypochlorite solution for 5 min, rinsed three times in sterile distilled water, air dried on sterilized filter paper, and plated on potato dextrose agar (PDA) and water agar (WA) amended with streptomycin sulfate. After 7 days incubation at 25C in the dark, isolates were preliminarily identified according to their morphological characters described by Leslie and Summerell (2006).
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum) in Serbia
IS  - 5
SP  - 1530
VL  - 106
DO  - 10.1094/PDIS-09-21-1998-PDN
ER  - 

@article{
author = "Živanov, Dalibor and Tančić-Živanov, Sonja and Savić, Aleksandra and Uhlarik, Ana and Miladinov, Zlatica and Medić-Pap, Slađana and Nagl, Nevena",
year = "2022",
abstract = "Chickpea (Cicer arietinum L.) is one of the most commonly consumed legume crops worldwide, cultivated in more than 55 countries (FAO 2017). However, in Serbia it is a novel crop grown on approximately 120 acres, but the area under this crop slightly increases each year. Fusarium wilt caused by F. oxysporum f. sp. ciceris is one of the most economically important diseases in most chickpea-growing areas, but there has been no formal report of the Fusarium wilt of chickpea in Serbia. In June 2018, the first symptoms of Fusarium wilt were registered at Rimski Sancevi (Vojvodina Province), Serbia (N 45'19.3119, E 19'49.9339), as wilted chickpea plants grouped in patches on approximately 5% of plants in a 3-acre area. Symptoms of yellowing and necrosis of foliage appeared as late wilt in the podding stage. Roots of affected plants showed no external discoloration, but a cross-section showed dark-brown discoloration of xylem tissue. To isolate the causal agent, symptomatic plants were collected and 10 cuttings of root symptomatic tissue were surface disinfected with 2% sodium hypochlorite solution for 5 min, rinsed three times in sterile distilled water, air dried on sterilized filter paper, and plated on potato dextrose agar (PDA) and water agar (WA) amended with streptomycin sulfate. After 7 days incubation at 25C in the dark, isolates were preliminarily identified according to their morphological characters described by Leslie and Summerell (2006).",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum) in Serbia",
number = "5",
pages = "1530",
volume = "106",
doi = "10.1094/PDIS-09-21-1998-PDN"
}

Živanov, D., Tančić-Živanov, S., Savić, A., Uhlarik, A., Miladinov, Z., Medić-Pap, S.,& Nagl, N.. (2022). First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum) in Serbia. in Plant Disease
The American Phytopathological Society., 106(5), 1530.
https://doi.org/10.1094/PDIS-09-21-1998-PDN

Živanov D, Tančić-Živanov S, Savić A, Uhlarik A, Miladinov Z, Medić-Pap S, Nagl N. First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum) in Serbia. in Plant Disease. 2022;106(5):1530.
doi:10.1094/PDIS-09-21-1998-PDN .

Živanov, Dalibor, Tančić-Živanov, Sonja, Savić, Aleksandra, Uhlarik, Ana, Miladinov, Zlatica, Medić-Pap, Slađana, Nagl, Nevena, "First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum) in Serbia" in Plant Disease, 106, no. 5 (2022):1530,
https://doi.org/10.1094/PDIS-09-21-1998-PDN . .

TY  - JOUR
AU  - Medić-Pap, Slađana
AU  - Živanov, Dalibor
AU  - Tančić-Živanov, Sonja
AU  - Pap, Predrag
AU  - Galović, Vladislava
AU  - Nagl, Nevena
AU  - Župunski, Vesna
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3177
AB  - Monitoring the health status of narrow-leaved ash tree seedlings (forest office Morović, locality Vinična) in the early spring of 2015, after the catastrophic floods in May 2014, revealed presence of large dark necrotic areas on 1–2 year old sprouts. The isolation of the fungal pathogen was done by standard phytopathological protocols. Three representative isolates (K41, K42 and K78) were preliminary detected and purified by a single-spore technique for further morphological, molecular analyses and pathogenicity testing. Morphological characteristics classified the isolates as Fusarium tricinctum. Tested isolates on narrow leaved ash sprouts caused reddish brown elongated necrotic lesions averaged 20.1 mm. Two marker genes, translation elongation factor 1-alpha (TEF1-α) and internal transcribed spacer (ITS1), were used in this study. Using the Basic Local Alignment Search Tool (BLAST) searching engine, nucleotide sequences were compared to all related sequences. Alignement score resulted in 98.9% identities with F. tricinctum for isolate K78, while isolates K41 and K42 showed 94.1% and 94.3% identities with F. tricinctum complex respectively. To the best of our knowledge, this is the first report of F. tricinctum pathogen infection on flood stressed narrow-leaved ash trees in Serbia.
AB  - Poljski jasen (Fraxinus angustifolia Vahl.) je drvenasta vrsta rasprostranjena u nizijskim šumama u Evropi. Region jugozapadnog Srema gde se nalaze najvrednije šume ove vrste u Srbiji, bio je izložen katastrofalnim poplavama u maju 2014. Praćenjem zdravstvenog stanja sadnica jasena (šuma Morović, lokalitet „Vinična”) u rano proleće 2015. godine otkriveno je prisustvo velikih tamnih nekrotičnih područja na izbojcima starim 1–2 godine. Sa obolelih izbojaka, standardnim fitopatološkim postupkom, urađena je izolacija patogena. Prema morfološkim karakteristikama dobijeni izolati su determinisani kao Fusarium tricinctum. Za dalje analize su uzeta tri reprezentativna monosporna izolata (K41, K42 i K78). BLAST pretragom NCBI nukleotidne baze utvrđeno je 98,9% sličnosti TEF1-α sekvence izolata K78 (MN822227.1) sa Fusarium tricinctum i 100% poklapanja ITS sekvence ovog izolata (GenBank MK928426.1) sa izolatom F. tricinctum ZMXR6 (MT446111.1). IGS sekvence izo lata K41 i K42 (MZ749901 i MZ749902) imale su 94,1% i 94,3% sličnosti sa F. tricinctum kompleksom, na osnovu BLAST pretrage FUSARIUM-ID nukleotidne baze. Testovi patogenosti na izbojcima jasena starim godinu dana pokazali su crvenkasto smeđe izdužene nekrotične lezije prosečne dužine 20,1 mm. Patogen je reizolovan iz izbojaka sa simptomima. Prema našim saznanjima, ovo je prvi nalaz F. tricinctum na stablima poljskog jasena u Srbiji.
PB  - Novi Sad : Matica srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia
T1  - Prvi nalaz Fusarium tricinctum na poljskom jasenu (Fraxinus angustifolia Vahl.) u Srbiji
EP  - 30
SP  - 19
VL  - 142
DO  - 10.2298/ZMSPN2242019M
ER  - 

@article{
author = "Medić-Pap, Slađana and Živanov, Dalibor and Tančić-Živanov, Sonja and Pap, Predrag and Galović, Vladislava and Nagl, Nevena and Župunski, Vesna",
year = "2022",
abstract = "Monitoring the health status of narrow-leaved ash tree seedlings (forest office Morović, locality Vinična) in the early spring of 2015, after the catastrophic floods in May 2014, revealed presence of large dark necrotic areas on 1–2 year old sprouts. The isolation of the fungal pathogen was done by standard phytopathological protocols. Three representative isolates (K41, K42 and K78) were preliminary detected and purified by a single-spore technique for further morphological, molecular analyses and pathogenicity testing. Morphological characteristics classified the isolates as Fusarium tricinctum. Tested isolates on narrow leaved ash sprouts caused reddish brown elongated necrotic lesions averaged 20.1 mm. Two marker genes, translation elongation factor 1-alpha (TEF1-α) and internal transcribed spacer (ITS1), were used in this study. Using the Basic Local Alignment Search Tool (BLAST) searching engine, nucleotide sequences were compared to all related sequences. Alignement score resulted in 98.9% identities with F. tricinctum for isolate K78, while isolates K41 and K42 showed 94.1% and 94.3% identities with F. tricinctum complex respectively. To the best of our knowledge, this is the first report of F. tricinctum pathogen infection on flood stressed narrow-leaved ash trees in Serbia., Poljski jasen (Fraxinus angustifolia Vahl.) je drvenasta vrsta rasprostranjena u nizijskim šumama u Evropi. Region jugozapadnog Srema gde se nalaze najvrednije šume ove vrste u Srbiji, bio je izložen katastrofalnim poplavama u maju 2014. Praćenjem zdravstvenog stanja sadnica jasena (šuma Morović, lokalitet „Vinična”) u rano proleće 2015. godine otkriveno je prisustvo velikih tamnih nekrotičnih područja na izbojcima starim 1–2 godine. Sa obolelih izbojaka, standardnim fitopatološkim postupkom, urađena je izolacija patogena. Prema morfološkim karakteristikama dobijeni izolati su determinisani kao Fusarium tricinctum. Za dalje analize su uzeta tri reprezentativna monosporna izolata (K41, K42 i K78). BLAST pretragom NCBI nukleotidne baze utvrđeno je 98,9% sličnosti TEF1-α sekvence izolata K78 (MN822227.1) sa Fusarium tricinctum i 100% poklapanja ITS sekvence ovog izolata (GenBank MK928426.1) sa izolatom F. tricinctum ZMXR6 (MT446111.1). IGS sekvence izo lata K41 i K42 (MZ749901 i MZ749902) imale su 94,1% i 94,3% sličnosti sa F. tricinctum kompleksom, na osnovu BLAST pretrage FUSARIUM-ID nukleotidne baze. Testovi patogenosti na izbojcima jasena starim godinu dana pokazali su crvenkasto smeđe izdužene nekrotične lezije prosečne dužine 20,1 mm. Patogen je reizolovan iz izbojaka sa simptomima. Prema našim saznanjima, ovo je prvi nalaz F. tricinctum na stablima poljskog jasena u Srbiji.",
publisher = "Novi Sad : Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia, Prvi nalaz Fusarium tricinctum na poljskom jasenu (Fraxinus angustifolia Vahl.) u Srbiji",
pages = "30-19",
volume = "142",
doi = "10.2298/ZMSPN2242019M"
}

Medić-Pap, S., Živanov, D., Tančić-Živanov, S., Pap, P., Galović, V., Nagl, N.,& Župunski, V.. (2022). First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia. in Zbornik Matice srpske za prirodne nauke
Novi Sad : Matica srpska., 142, 19-30.
https://doi.org/10.2298/ZMSPN2242019M

Medić-Pap S, Živanov D, Tančić-Živanov S, Pap P, Galović V, Nagl N, Župunski V. First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia. in Zbornik Matice srpske za prirodne nauke. 2022;142:19-30.
doi:10.2298/ZMSPN2242019M .

Medić-Pap, Slađana, Živanov, Dalibor, Tančić-Živanov, Sonja, Pap, Predrag, Galović, Vladislava, Nagl, Nevena, Župunski, Vesna, "First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia" in Zbornik Matice srpske za prirodne nauke, 142 (2022):19-30,
https://doi.org/10.2298/ZMSPN2242019M . .

TY  - CONF
AU  - Stankov Petreš, Aleksandra
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Nagl, Nevena
AU  - Grahovac, Mila
AU  - Ćurčić, Živko
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3368
AB  - Primena fungicida u suzbijanju zemljišnih patogena ne predstavlja efikasnu i ekonomski opravdanu meru. Takođe, oni mogu da naruše balans korisnih mikroorganizama u zemljištu, dok njihove rezidue utiču na zagađenje životne sredine. U nastojanju da se smanji upotreba sintetičkih fungicida, primena bioloških agenasa može imati važnu ulogu u „eco-frendly“ poljoprivrednim sistemima. Biološki agensi kao što su vrste iz rodova Bacillus i Trichoderma se najčešće upotrebljavaju u biološkoj borbi protiv zemljišnih patogena. Cilj ovog istraživanja je bio da se ispita antagonistički uticaj Bacillus sp. i Trichoderma spp. na izolate Macrophomina phaseolina (ŠR55(3)/09 i ŠR23/11) u in vitro uslovima. Za ispitivanje je korišten Bacillus sp., 74 koji je komercijalno dostupan kao mikrobiološko đubrivo pod nazivom Bacillomix® Original (Bacillomix Co., Srbija). Metodom inkorporacije komercijalne suspenzije mikrobiološkog đubriva u KDA hranljivu podlogu ispitane su koncentracije preparata od 1, 2, 5 i 10%. U cilju ispitivanja osetljivosti M. phaseolina prema Trichoderma spp. korišteno je 13 izolata koji su predhodno identifikovani kao Trichoderma harzianum. Test dvojne kulture (TDK) je korišten kako bi utvrdili antagonistički uticaj ispitivanih izolata.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.
T1  - Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro
EP  - 74
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3368
ER  - 

@conference{
author = "Stankov Petreš, Aleksandra and Budakov, Dragana and Stojšin, Vera and Nagl, Nevena and Grahovac, Mila and Ćurčić, Živko",
year = "2022",
abstract = "Primena fungicida u suzbijanju zemljišnih patogena ne predstavlja efikasnu i ekonomski opravdanu meru. Takođe, oni mogu da naruše balans korisnih mikroorganizama u zemljištu, dok njihove rezidue utiču na zagađenje životne sredine. U nastojanju da se smanji upotreba sintetičkih fungicida, primena bioloških agenasa može imati važnu ulogu u „eco-frendly“ poljoprivrednim sistemima. Biološki agensi kao što su vrste iz rodova Bacillus i Trichoderma se najčešće upotrebljavaju u biološkoj borbi protiv zemljišnih patogena. Cilj ovog istraživanja je bio da se ispita antagonistički uticaj Bacillus sp. i Trichoderma spp. na izolate Macrophomina phaseolina (ŠR55(3)/09 i ŠR23/11) u in vitro uslovima. Za ispitivanje je korišten Bacillus sp., 74 koji je komercijalno dostupan kao mikrobiološko đubrivo pod nazivom Bacillomix® Original (Bacillomix Co., Srbija). Metodom inkorporacije komercijalne suspenzije mikrobiološkog đubriva u KDA hranljivu podlogu ispitane su koncentracije preparata od 1, 2, 5 i 10%. U cilju ispitivanja osetljivosti M. phaseolina prema Trichoderma spp. korišteno je 13 izolata koji su predhodno identifikovani kao Trichoderma harzianum. Test dvojne kulture (TDK) je korišten kako bi utvrdili antagonistički uticaj ispitivanih izolata.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.",
title = "Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro",
pages = "74-73",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3368"
}

Stankov Petreš, A., Budakov, D., Stojšin, V., Nagl, N., Grahovac, M.,& Ćurčić, Ž.. (2022). Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro. in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.
Beograd : Društvo za zaštitu bilja Srbije., 73-74.
https://hdl.handle.net/21.15107/rcub_fiver_3368

Stankov Petreš A, Budakov D, Stojšin V, Nagl N, Grahovac M, Ćurčić Ž. Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro. in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.. 2022;:73-74.
https://hdl.handle.net/21.15107/rcub_fiver_3368 .

Stankov Petreš, Aleksandra, Budakov, Dragana, Stojšin, Vera, Nagl, Nevena, Grahovac, Mila, Ćurčić, Živko, "Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro" in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022. (2022):73-74,
https://hdl.handle.net/21.15107/rcub_fiver_3368 .

TY  - JOUR
AU  - Nagl, Nevena
AU  - Kuzmanović, Boris
AU  - Zanetti, Federica
AU  - Vollmann, Johann
AU  - Marjanović-Jeromela, Ana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3348
AB  - Camelina sativa L. is one of the oldest crops of the Brassicaceae family, first domesticated in the region of south-eastern Europe. It has regained interest as a very promising alternative oilseed crop with broad adaptability, a wide range of tolerances to pests and diseases, and low-input requirements. The genetic diversity in spring camelina proved to be limited, so the identification and characterization of genetic variations in germplasm originating from different sources is considered very useful for development of efficient breeding programmes. The aim of the study was to use SSR markers in order to investigate genetic variation of twenty spring camelina accessions of different origin and estimate their genetic relatedness. Forty-five individual samples were taken from each accession and used for amplification of SSR markers P4C11, P6E4 and LIB19. Percentage of polymorphic loci, number of alleles, effective number of alleles, expected heterozygosity and Shannon’s information index were used to estimate genetic variation. The accessions expressed different levels of genetic variation. The highest variability was found in cultivar Zavolzskij, breeding line CK2X-7, cultivar NS Zlatka and breeding line CK2X-9. The most uniform were cultivar Pernice, and population Maslomania. AMOVA (analysis of molecular variance) showed that 64% of the total genetic variation was attributed to variance within accessions and 36% to variance among them. IBased on genetic distance, accessions were divided in two clades, which both were further divided in two subclades. Genetic distance analysis indicated that there was overlapping in certain breeding programs and exchange of breeding germplasm.
AB  - Camelina sativa L., ili lanik, je jedna od najstarijih gajenih kupusnjača, koja je prvo bila domestifikovana na prostorima Jugoistočne Evrope. U poslednjih dvadeset godina, obnovljen je interes za ovu, skoro zaboravljenu biljnu vrstu, kao potencijalnu alternativnu uljanu kulturu. Razlozi za to su visok stepen adaptabilnosti, tolerantnost na mnoge štetočine i bolesti i nizak stepen potrebnih ulaganja za njeno gajenje. Genetička varijabilnost jarog lanika je relativno ograničena, tako da se njeno ispitivanje smatra izuzetno važnim za razvoj efikasnih programa oplemenjivanja ove kulture. Cilj istraživanja je bio ispitivanje genetičke varijabilnosti i srodnosti dvadeset genotipova jarog lanika, pomoću mikrosatelitskih (SSR) markera. Od svakog genotipa uzeto je 45 individualnih uzoraka, koji su korišćeni za amplifikaciju SSR prajmerima P4C11, P6E4 i LIB19. Ispitivani genotipovi su ispoljili različite stepene genetičke varijabilnosti, a AMOV (analiza molekularne varijanse) je ukazala da je 64% od ukupne genetičke varijabilnosti pripadalo varijabilnosti unutar genotipova. Analizom genetičke distance se može videti da je došlo do preklapanja različitih programa oplemenjivanja, kao i da je dolazilo do razmene germplazme između njih.
PB  - Novi Sad : Institute of Field and Vegetable Crops
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Genetic variation and relationships among spring camelina (Camelina sativa, Brassicaceae) accessions of different origin
T1  - Genetička varijabilnost i srodnost genotipova jarog lanika različitog porekla
EP  - 90
IS  - 3
SP  - 86
VL  - 59
DO  - 10.5937/ratpov59-38897
ER  - 

@article{
author = "Nagl, Nevena and Kuzmanović, Boris and Zanetti, Federica and Vollmann, Johann and Marjanović-Jeromela, Ana",
year = "2022",
abstract = "Camelina sativa L. is one of the oldest crops of the Brassicaceae family, first domesticated in the region of south-eastern Europe. It has regained interest as a very promising alternative oilseed crop with broad adaptability, a wide range of tolerances to pests and diseases, and low-input requirements. The genetic diversity in spring camelina proved to be limited, so the identification and characterization of genetic variations in germplasm originating from different sources is considered very useful for development of efficient breeding programmes. The aim of the study was to use SSR markers in order to investigate genetic variation of twenty spring camelina accessions of different origin and estimate their genetic relatedness. Forty-five individual samples were taken from each accession and used for amplification of SSR markers P4C11, P6E4 and LIB19. Percentage of polymorphic loci, number of alleles, effective number of alleles, expected heterozygosity and Shannon’s information index were used to estimate genetic variation. The accessions expressed different levels of genetic variation. The highest variability was found in cultivar Zavolzskij, breeding line CK2X-7, cultivar NS Zlatka and breeding line CK2X-9. The most uniform were cultivar Pernice, and population Maslomania. AMOVA (analysis of molecular variance) showed that 64% of the total genetic variation was attributed to variance within accessions and 36% to variance among them. IBased on genetic distance, accessions were divided in two clades, which both were further divided in two subclades. Genetic distance analysis indicated that there was overlapping in certain breeding programs and exchange of breeding germplasm., Camelina sativa L., ili lanik, je jedna od najstarijih gajenih kupusnjača, koja je prvo bila domestifikovana na prostorima Jugoistočne Evrope. U poslednjih dvadeset godina, obnovljen je interes za ovu, skoro zaboravljenu biljnu vrstu, kao potencijalnu alternativnu uljanu kulturu. Razlozi za to su visok stepen adaptabilnosti, tolerantnost na mnoge štetočine i bolesti i nizak stepen potrebnih ulaganja za njeno gajenje. Genetička varijabilnost jarog lanika je relativno ograničena, tako da se njeno ispitivanje smatra izuzetno važnim za razvoj efikasnih programa oplemenjivanja ove kulture. Cilj istraživanja je bio ispitivanje genetičke varijabilnosti i srodnosti dvadeset genotipova jarog lanika, pomoću mikrosatelitskih (SSR) markera. Od svakog genotipa uzeto je 45 individualnih uzoraka, koji su korišćeni za amplifikaciju SSR prajmerima P4C11, P6E4 i LIB19. Ispitivani genotipovi su ispoljili različite stepene genetičke varijabilnosti, a AMOV (analiza molekularne varijanse) je ukazala da je 64% od ukupne genetičke varijabilnosti pripadalo varijabilnosti unutar genotipova. Analizom genetičke distance se može videti da je došlo do preklapanja različitih programa oplemenjivanja, kao i da je dolazilo do razmene germplazme između njih.",
publisher = "Novi Sad : Institute of Field and Vegetable Crops",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Genetic variation and relationships among spring camelina (Camelina sativa, Brassicaceae) accessions of different origin, Genetička varijabilnost i srodnost genotipova jarog lanika različitog porekla",
pages = "90-86",
number = "3",
volume = "59",
doi = "10.5937/ratpov59-38897"
}

Nagl, N., Kuzmanović, B., Zanetti, F., Vollmann, J.,& Marjanović-Jeromela, A.. (2022). Genetic variation and relationships among spring camelina (Camelina sativa, Brassicaceae) accessions of different origin. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Novi Sad : Institute of Field and Vegetable Crops., 59(3), 86-90.
https://doi.org/10.5937/ratpov59-38897

Nagl N, Kuzmanović B, Zanetti F, Vollmann J, Marjanović-Jeromela A. Genetic variation and relationships among spring camelina (Camelina sativa, Brassicaceae) accessions of different origin. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2022;59(3):86-90.
doi:10.5937/ratpov59-38897 .

Nagl, Nevena, Kuzmanović, Boris, Zanetti, Federica, Vollmann, Johann, Marjanović-Jeromela, Ana, "Genetic variation and relationships among spring camelina (Camelina sativa, Brassicaceae) accessions of different origin" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 59, no. 3 (2022):86-90,
https://doi.org/10.5937/ratpov59-38897 . .

TY  - CONF
AU  - Nagl, Nevena
AU  - Sinkovič, Lovro
AU  - Vasić, Mirjana
AU  - Meglič, Vladimir
AU  - Savić, Aleksandra
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Pipan, Barbara
AU  - Marjanović-Jeromela, Ana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2804
AB  - Grass pea (Lathyrus sativus L.) is a legume with high protein content and good quantities of essential amino acids. It has high tolerance to drought, high adaptability to extreme conditions, disease resistance and low input requirement for cultivation, which makes it very useful for cultivation by resource-poor farmers. Potential usage of grass pea is limited due to the presence of anti-nutritional factors including protease inhibitors, especially trypsin inhibitors. They have a major impact on nutritional value, by inhibiting pancreatic serine proteases (thus impairing protein digestion), but are also likely to protect legume seed against attack by predators. Although once extensively grown throughout Balkan Peninsula, grass pea was nearly forgotten. In recent years there was an effort to reintroduce and increase the interest of researchers and farmers in this crop. The aim of this study was to establish whether genotype and environment have significant influences on trypsin inhibitor activity (TIA) in grass pea and to determine whether TIA is correlated with seed protein content.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
T1  - Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity
EP  - 82
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2804
ER  - 

@conference{
author = "Nagl, Nevena and Sinkovič, Lovro and Vasić, Mirjana and Meglič, Vladimir and Savić, Aleksandra and Nikolić, Zorica and Petrović, Gordana and Pipan, Barbara and Marjanović-Jeromela, Ana",
year = "2022",
abstract = "Grass pea (Lathyrus sativus L.) is a legume with high protein content and good quantities of essential amino acids. It has high tolerance to drought, high adaptability to extreme conditions, disease resistance and low input requirement for cultivation, which makes it very useful for cultivation by resource-poor farmers. Potential usage of grass pea is limited due to the presence of anti-nutritional factors including protease inhibitors, especially trypsin inhibitors. They have a major impact on nutritional value, by inhibiting pancreatic serine proteases (thus impairing protein digestion), but are also likely to protect legume seed against attack by predators. Although once extensively grown throughout Balkan Peninsula, grass pea was nearly forgotten. In recent years there was an effort to reintroduce and increase the interest of researchers and farmers in this crop. The aim of this study was to establish whether genotype and environment have significant influences on trypsin inhibitor activity (TIA) in grass pea and to determine whether TIA is correlated with seed protein content.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina",
title = "Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2804"
}

Nagl, N., Sinkovič, L., Vasić, M., Meglič, V., Savić, A., Nikolić, Z., Petrović, G., Pipan, B.,& Marjanović-Jeromela, A.. (2022). Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 82-82.
https://hdl.handle.net/21.15107/rcub_fiver_2804

Nagl N, Sinkovič L, Vasić M, Meglič V, Savić A, Nikolić Z, Petrović G, Pipan B, Marjanović-Jeromela A. Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina. 2022;:82-82.
https://hdl.handle.net/21.15107/rcub_fiver_2804 .

Nagl, Nevena, Sinkovič, Lovro, Vasić, Mirjana, Meglič, Vladimir, Savić, Aleksandra, Nikolić, Zorica, Petrović, Gordana, Pipan, Barbara, Marjanović-Jeromela, Ana, "Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity" in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina (2022):82-82,
https://hdl.handle.net/21.15107/rcub_fiver_2804 .

TY  - CONF
AU  - Pipan, Barbara
AU  - Antić, Marina
AU  - Rajković, Dragana
AU  - Sinkovič, Lovro
AU  - Rašeta, Sonja
AU  - Nagl, Nevena
AU  - Goreta Ban, Smiljana
AU  - Meglič, Vladimir
AU  - Todorović, Vida
AU  - Marjanović-Jeromela, Ana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2805
AB  - Family Brassicaceae is abundant in species used in food production, but also contains many industrial species, vegetables and ornamentals. Green collard (Brassica oleracea var. acephala L.) was traditionally grown in Balkan Peninsula and its wide green leaves, rich in vitamins A and C, are used for cooking. There is great diversity in B. oleracea acephala germplasm that reflects on agronomic, morphological and molecular characteristics. In this study, was assessed genetic variation of 38 accessions of green collard out of which 3 accessions were standard/commercial varieties and 35 were locally grown populations, collected in Western Balkan: 18 from Bosnia and Herzegovina, 8 from Serbia (Vojvodina), 7 from Croatia and two from Montenegro. Genetic diversity was assessed using twelve species-specific SSR markers (Simple Sequence Repeats) originating from B. oleracea (8), B. rapa (2) and B. napus (2). Each accession was represented with four individual samples.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
T1  - Assessment of molecular diversity of Brassica oleracea var. acephala
EP  - 138
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2805
ER  - 

@conference{
author = "Pipan, Barbara and Antić, Marina and Rajković, Dragana and Sinkovič, Lovro and Rašeta, Sonja and Nagl, Nevena and Goreta Ban, Smiljana and Meglič, Vladimir and Todorović, Vida and Marjanović-Jeromela, Ana",
year = "2022",
abstract = "Family Brassicaceae is abundant in species used in food production, but also contains many industrial species, vegetables and ornamentals. Green collard (Brassica oleracea var. acephala L.) was traditionally grown in Balkan Peninsula and its wide green leaves, rich in vitamins A and C, are used for cooking. There is great diversity in B. oleracea acephala germplasm that reflects on agronomic, morphological and molecular characteristics. In this study, was assessed genetic variation of 38 accessions of green collard out of which 3 accessions were standard/commercial varieties and 35 were locally grown populations, collected in Western Balkan: 18 from Bosnia and Herzegovina, 8 from Serbia (Vojvodina), 7 from Croatia and two from Montenegro. Genetic diversity was assessed using twelve species-specific SSR markers (Simple Sequence Repeats) originating from B. oleracea (8), B. rapa (2) and B. napus (2). Each accession was represented with four individual samples.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina",
title = "Assessment of molecular diversity of Brassica oleracea var. acephala",
pages = "138-137",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2805"
}

Pipan, B., Antić, M., Rajković, D., Sinkovič, L., Rašeta, S., Nagl, N., Goreta Ban, S., Meglič, V., Todorović, V.,& Marjanović-Jeromela, A.. (2022). Assessment of molecular diversity of Brassica oleracea var. acephala. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 137-138.
https://hdl.handle.net/21.15107/rcub_fiver_2805

Pipan B, Antić M, Rajković D, Sinkovič L, Rašeta S, Nagl N, Goreta Ban S, Meglič V, Todorović V, Marjanović-Jeromela A. Assessment of molecular diversity of Brassica oleracea var. acephala. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina. 2022;:137-138.
https://hdl.handle.net/21.15107/rcub_fiver_2805 .

Pipan, Barbara, Antić, Marina, Rajković, Dragana, Sinkovič, Lovro, Rašeta, Sonja, Nagl, Nevena, Goreta Ban, Smiljana, Meglič, Vladimir, Todorović, Vida, Marjanović-Jeromela, Ana, "Assessment of molecular diversity of Brassica oleracea var. acephala" in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina (2022):137-138,
https://hdl.handle.net/21.15107/rcub_fiver_2805 .

TY  - CONF
AU  - Gvozdenac, Sonja
AU  - Ilić, Aleksandra
AU  - Vasić, Mirjana
AU  - Nagl, Nevena
AU  - Prvulović, Dejan
AU  - Petrović, Gordana
AU  - Tanasković, Snežana
AU  - Vukajlović, Filip
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3143
AB  - Legumes represent a valuable source of proteins in human and animal nutrition, as well as of different nutrients. Also, they enable diversification of agroecosystems and can be considered climate smart crops. Therefore, they are gaining importance in both developed and developing countries. The most important, and often limiting factor in legume production is the presence of the bean weevil (Acanthoscelides obtectus). This work tested the suitability of three different legume species, i. e. common bean, grass pea and faba bean (two accessions of each species) with growing interest in human nutrition, for the development and population growth of this pest, with special emphasis on the levels of anti-nutritive compounds – trypsin inhibitors. The suitability was assessed based on the progeny production after each month. The development and progeny production were significantly affected by the legume species, but also by the accessions. The highest number of emerged adults was on common bean, while the lowest regardless on observation period, was on faba bean, indicating its low suitability for weevil development. Correlation analysis detected significant influence of the level of trypsin inhibitors on progeny production.
PB  - International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Palearctic Regional Section (IOBC-WPRS)
C3  - Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain
T1  - Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?
EP  - 70
SP  - 64
VL  - 159
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3143
ER  - 

@conference{
author = "Gvozdenac, Sonja and Ilić, Aleksandra and Vasić, Mirjana and Nagl, Nevena and Prvulović, Dejan and Petrović, Gordana and Tanasković, Snežana and Vukajlović, Filip",
year = "2022",
abstract = "Legumes represent a valuable source of proteins in human and animal nutrition, as well as of different nutrients. Also, they enable diversification of agroecosystems and can be considered climate smart crops. Therefore, they are gaining importance in both developed and developing countries. The most important, and often limiting factor in legume production is the presence of the bean weevil (Acanthoscelides obtectus). This work tested the suitability of three different legume species, i. e. common bean, grass pea and faba bean (two accessions of each species) with growing interest in human nutrition, for the development and population growth of this pest, with special emphasis on the levels of anti-nutritive compounds – trypsin inhibitors. The suitability was assessed based on the progeny production after each month. The development and progeny production were significantly affected by the legume species, but also by the accessions. The highest number of emerged adults was on common bean, while the lowest regardless on observation period, was on faba bean, indicating its low suitability for weevil development. Correlation analysis detected significant influence of the level of trypsin inhibitors on progeny production.",
publisher = "International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Palearctic Regional Section (IOBC-WPRS)",
journal = "Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain",
title = "Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?",
pages = "70-64",
volume = "159",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3143"
}

Gvozdenac, S., Ilić, A., Vasić, M., Nagl, N., Prvulović, D., Petrović, G., Tanasković, S.,& Vukajlović, F.. (2022). Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?. in Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain
International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Palearctic Regional Section (IOBC-WPRS)., 159, 64-70.
https://hdl.handle.net/21.15107/rcub_fiver_3143

Gvozdenac S, Ilić A, Vasić M, Nagl N, Prvulović D, Petrović G, Tanasković S, Vukajlović F. Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?. in Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain. 2022;159:64-70.
https://hdl.handle.net/21.15107/rcub_fiver_3143 .

Gvozdenac, Sonja, Ilić, Aleksandra, Vasić, Mirjana, Nagl, Nevena, Prvulović, Dejan, Petrović, Gordana, Tanasković, Snežana, Vukajlović, Filip, "Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?" in Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain, 159 (2022):64-70,
https://hdl.handle.net/21.15107/rcub_fiver_3143 .

TY  - JOUR
AU  - Vukotić, Jelena
AU  - Stojšin, Vera
AU  - Nagl, Nevena
AU  - Petreš, Mladen
AU  - Hrustić, jovana
AU  - Grahovac, Mila
AU  - Tanović, Boris
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3984
AB  - Neofabraea spp. is one of the major causal agents of the postharvest decay of apple fruit.
Bull’s eye rot can cause fruit rot, as well as tree canker. In Serbia, during February and March, 2017 and 2018, 56.3% of collected stored rotten apple fruits were found to be infected with Neofabraea spp. Using polymerase chain reaction, phylogenetic analysis, and morphological characterization, Neofabraea alba was identified as the causal agent of Bull’s eye rot. Twenty-five selected isolates were analyzed by DNA sequencing of the internal transcribed spacer and 16 S mitochondrial ribosomal RNA. The phylogenetic analyses provided information on the distance between Neofabraea spp., while merged phylogenetic tree was required to give insight and clarify the relationships between isolates of Neofabraea alba. N. alba demonstrated pathogenicity on apple fruit as well as on branches, with significantly different aggressiveness among the isolates. Isolates were able to produce larger cankers and acervuli on apple branches with abundant sporulation All identified isolates were separated into two morphotypes and described based on their macromorphological characteristics on culture media. It was noticed that different culture media, pH, and temperature could affect macromorphological characteristics and development. Representative isolates were able to grow at a temperature range of 0 to 25  C, above which only one isolate was able to grow on culture media. All isolates showed growth at a pH range of 2–10, with significant differences detected between isolates at different pH values. The type of cultivation medium significantly affected the size and shape of conidia, while the presence of the microconidia was recorded within isolates. The aim of this study was to identify the causal agents of Bull’s eye rot on stored apples in Serbia in the period 2017–2018 and characterize the obtained isolates based on pathogenic, morphological, physiological, and molecular criteria.
PB  - Basel : MDPI
T2  - Agronomy - Basel
T1  - Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia
IS  - 9
SP  - 2015
VL  - 12
DO  - 10.3390/agronomy12092015
ER  - 

@article{
author = "Vukotić, Jelena and Stojšin, Vera and Nagl, Nevena and Petreš, Mladen and Hrustić, jovana and Grahovac, Mila and Tanović, Boris",
year = "2022",
abstract = "Neofabraea spp. is one of the major causal agents of the postharvest decay of apple fruit.
Bull’s eye rot can cause fruit rot, as well as tree canker. In Serbia, during February and March, 2017 and 2018, 56.3% of collected stored rotten apple fruits were found to be infected with Neofabraea spp. Using polymerase chain reaction, phylogenetic analysis, and morphological characterization, Neofabraea alba was identified as the causal agent of Bull’s eye rot. Twenty-five selected isolates were analyzed by DNA sequencing of the internal transcribed spacer and 16 S mitochondrial ribosomal RNA. The phylogenetic analyses provided information on the distance between Neofabraea spp., while merged phylogenetic tree was required to give insight and clarify the relationships between isolates of Neofabraea alba. N. alba demonstrated pathogenicity on apple fruit as well as on branches, with significantly different aggressiveness among the isolates. Isolates were able to produce larger cankers and acervuli on apple branches with abundant sporulation All identified isolates were separated into two morphotypes and described based on their macromorphological characteristics on culture media. It was noticed that different culture media, pH, and temperature could affect macromorphological characteristics and development. Representative isolates were able to grow at a temperature range of 0 to 25  C, above which only one isolate was able to grow on culture media. All isolates showed growth at a pH range of 2–10, with significant differences detected between isolates at different pH values. The type of cultivation medium significantly affected the size and shape of conidia, while the presence of the microconidia was recorded within isolates. The aim of this study was to identify the causal agents of Bull’s eye rot on stored apples in Serbia in the period 2017–2018 and characterize the obtained isolates based on pathogenic, morphological, physiological, and molecular criteria.",
publisher = "Basel : MDPI",
journal = "Agronomy - Basel",
title = "Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia",
number = "9",
pages = "2015",
volume = "12",
doi = "10.3390/agronomy12092015"
}

Vukotić, J., Stojšin, V., Nagl, N., Petreš, M., Hrustić, j., Grahovac, M.,& Tanović, B.. (2022). Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia. in Agronomy - Basel
Basel : MDPI., 12(9), 2015.
https://doi.org/10.3390/agronomy12092015

Vukotić J, Stojšin V, Nagl N, Petreš M, Hrustić J, Grahovac M, Tanović B. Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia. in Agronomy - Basel. 2022;12(9):2015.
doi:10.3390/agronomy12092015 .

Vukotić, Jelena, Stojšin, Vera, Nagl, Nevena, Petreš, Mladen, Hrustić, jovana, Grahovac, Mila, Tanović, Boris, "Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia" in Agronomy - Basel, 12, no. 9 (2022):2015,
https://doi.org/10.3390/agronomy12092015 . .

TY  - JOUR
AU  - Živanov, Dalibor
AU  - Tančić-Živanov, Sonja
AU  - Savić, Aleksandra
AU  - Uhlarik, Ana
AU  - Mamlić, Zlatica
AU  - Medić-Pap, Slađana
AU  - Nagl, Nevena
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2525
AB  - Chickpea (Cicer arietinum L.) is one of the most commonly consumed legume crops worldwide However, in Serbia, it is a novel crop grown on approximately 120 acres. Fusarium wilt caused by F. oxysporum f. sp. ciceris is one of the most economically important disease in the most chickpea-growing areas, but yet there has been no formal report of the Fusarium wilt of chickpea in Serbia. In June 2018, the first symptoms of Fusarium wilt were registered as wilted chickpea plants grouped in patches on approximately 5% plants in the area of 3 acres. Symptoms as yellowing and necrosis of foliage appeared as late wilt in the podding stage. Roots of affected plants showed no external discoloration, but cross-sections showed darkbrown discoloration of xylem tissue. To isolate the causal agent, symptomatic plants were collected, and ten cuttings of symptomatic root tissue were surface disinfected with 2% sodium hypochlorite solution for 5 min, rinsed three times in sterile distilled water, air-dried on sterilized filter paper and plated on potato dextrose agar (PDA) and water agar (WA) amended with streptomycin sulfate. After seven days of incubation, isolates were preliminarily identified according to their morphological characters and Fusarium Laboratory Manual. For each isolate 100, micro/macroconidia and chlamydospores were measured. Conidia were hyaline; macroconidia sickle-shaped, with blunt ends, 2-4 septa (10.1-17.7 x 3.1-5.8 μm); microconidia ellipsoidal, 0-1 septa (4.9-8.6-2.7-3.5 μm). Chlamydospores were globose (4.3-8.8 μm). Representative isolates (K343, K375 and K378) were purified by a single-spore technique for further analyses (Leslie and Summerell, 2006). Molecular identification of three representative isolates (K343, K375 and K378) was made by sequencing the rRNA internal transcribed spacer (ITS) region and translation elongation factor 1α (TEF1) gen. For all isolates, the ITS and TEF1 genes were amplified and sequenced with primers ITS1/4 EF1-728 and EF1-986. Based on a BLAST search of the NCBI nucleotide database, the ITS sequences (GenBank MK920204.1, MK928423.1 and MK928424.1) had 99.8% identity with F. oxysporum f. sp. ciceris isolate (MK074845.1). The TEF1 (GenBank MN788462.1, MN788463.1 and MN788464.1) had 96.3 -100% identities with F. oxysporum f. sp. ciceris isolate (FJ538245.1). The pathogenicity test was conducted on 7-day old plants according to the drench method. The concentration of inoculation suspensions was adjusted on 1x106, and ten plants per isolate were tested and inoculated with 10 ml of suspension. Control plants were drenched with 10 ml of sterilized distilled water. Nine days after inoculation, first symptoms as leaves wilting and white mycelia presence around stem base occurred on plants inoculated with isolate K378. On day 11, the first symptoms occurred on plants inoculated with isolates K343 and K375 as well. Up to the 13th day after inoculation, all plants were wilted, and the pathogen was successfully reisolated and confirmed as F. oxysporum f.sp. ciceris. To the best of our knowledge, this is the first report of F. oxysporum f.sp. ciceris causing Fusarium wilt on chickpea in Serbia.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia
IS  - 5
SP  - 1530
VL  - 106
DO  - 10.1094/PDIS-09-21-1998-PDN
ER  - 

@article{
author = "Živanov, Dalibor and Tančić-Živanov, Sonja and Savić, Aleksandra and Uhlarik, Ana and Mamlić, Zlatica and Medić-Pap, Slađana and Nagl, Nevena",
year = "2022",
abstract = "Chickpea (Cicer arietinum L.) is one of the most commonly consumed legume crops worldwide However, in Serbia, it is a novel crop grown on approximately 120 acres. Fusarium wilt caused by F. oxysporum f. sp. ciceris is one of the most economically important disease in the most chickpea-growing areas, but yet there has been no formal report of the Fusarium wilt of chickpea in Serbia. In June 2018, the first symptoms of Fusarium wilt were registered as wilted chickpea plants grouped in patches on approximately 5% plants in the area of 3 acres. Symptoms as yellowing and necrosis of foliage appeared as late wilt in the podding stage. Roots of affected plants showed no external discoloration, but cross-sections showed darkbrown discoloration of xylem tissue. To isolate the causal agent, symptomatic plants were collected, and ten cuttings of symptomatic root tissue were surface disinfected with 2% sodium hypochlorite solution for 5 min, rinsed three times in sterile distilled water, air-dried on sterilized filter paper and plated on potato dextrose agar (PDA) and water agar (WA) amended with streptomycin sulfate. After seven days of incubation, isolates were preliminarily identified according to their morphological characters and Fusarium Laboratory Manual. For each isolate 100, micro/macroconidia and chlamydospores were measured. Conidia were hyaline; macroconidia sickle-shaped, with blunt ends, 2-4 septa (10.1-17.7 x 3.1-5.8 μm); microconidia ellipsoidal, 0-1 septa (4.9-8.6-2.7-3.5 μm). Chlamydospores were globose (4.3-8.8 μm). Representative isolates (K343, K375 and K378) were purified by a single-spore technique for further analyses (Leslie and Summerell, 2006). Molecular identification of three representative isolates (K343, K375 and K378) was made by sequencing the rRNA internal transcribed spacer (ITS) region and translation elongation factor 1α (TEF1) gen. For all isolates, the ITS and TEF1 genes were amplified and sequenced with primers ITS1/4 EF1-728 and EF1-986. Based on a BLAST search of the NCBI nucleotide database, the ITS sequences (GenBank MK920204.1, MK928423.1 and MK928424.1) had 99.8% identity with F. oxysporum f. sp. ciceris isolate (MK074845.1). The TEF1 (GenBank MN788462.1, MN788463.1 and MN788464.1) had 96.3 -100% identities with F. oxysporum f. sp. ciceris isolate (FJ538245.1). The pathogenicity test was conducted on 7-day old plants according to the drench method. The concentration of inoculation suspensions was adjusted on 1x106, and ten plants per isolate were tested and inoculated with 10 ml of suspension. Control plants were drenched with 10 ml of sterilized distilled water. Nine days after inoculation, first symptoms as leaves wilting and white mycelia presence around stem base occurred on plants inoculated with isolate K378. On day 11, the first symptoms occurred on plants inoculated with isolates K343 and K375 as well. Up to the 13th day after inoculation, all plants were wilted, and the pathogen was successfully reisolated and confirmed as F. oxysporum f.sp. ciceris. To the best of our knowledge, this is the first report of F. oxysporum f.sp. ciceris causing Fusarium wilt on chickpea in Serbia.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia",
number = "5",
pages = "1530",
volume = "106",
doi = "10.1094/PDIS-09-21-1998-PDN"
}

Živanov, D., Tančić-Živanov, S., Savić, A., Uhlarik, A., Mamlić, Z., Medić-Pap, S.,& Nagl, N.. (2022). First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia. in Plant Disease
American Phytopathological Society., 106(5), 1530.
https://doi.org/10.1094/PDIS-09-21-1998-PDN

Živanov D, Tančić-Živanov S, Savić A, Uhlarik A, Mamlić Z, Medić-Pap S, Nagl N. First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia. in Plant Disease. 2022;106(5):1530.
doi:10.1094/PDIS-09-21-1998-PDN .

Živanov, Dalibor, Tančić-Živanov, Sonja, Savić, Aleksandra, Uhlarik, Ana, Mamlić, Zlatica, Medić-Pap, Slađana, Nagl, Nevena, "First report of Fusarium oxysporum f. sp. ciceris on chickpea (Cicer arietinum L.) in Serbia" in Plant Disease, 106, no. 5 (2022):1530,
https://doi.org/10.1094/PDIS-09-21-1998-PDN . .

TY  - CONF
AU  - Glogovac, Svetlana
AU  - Gvozdanović-Varga, Jelica
AU  - Zorić, Miroslav
AU  - Nagl, Nevena
AU  - Trkulja, Dragana
AU  - Brbaklić, Ljiljana
AU  - Kondić-Špika, Ankica
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2489
AB  - Garlic (Allium sativum L.) is one of the most important Allium species in terms of worldwide production and various usages in human nutrition, medicine, pharmacy and cosmetics. Basic method of garlic propagation is vegetative and creation of new varieties is mainly achieved by clonal selection. The characterization and preservation of samples in germplasm collections is of crucial importance in plant breeding, as well as availability of information about number and characteristics of samples in gene banks. Since pheotypic traits can vary significantly under the influence of environmental factors the characterization is more reliable by using DNA markers. Effective characterization of samples in collections and identification of duplicates is important from the economic aspect, ie. space saving and maintenance costs. The garlic collection of the Institute of Field and Vegetable Crops in Novi Sad (IFVCNS) includes 63 samples of autumn and 67 samples of spring garlic. These genotypes represent a valuable genetic pool for the selection of clones with appropriate characteristics, highly adapted for the production in the agro-climatic conditions of Serbia. Molecular characterization will provide more complete insight into diversity of samples, identification of potential duplicates and enable breeders more efficient selection and development of new cultivars.
PB  - Novi Sad : University of Novi Sad, Faculty of Sciences
C3  - Book of Abstracts, International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference, 25-26 November 2021, Novi Sad, Serbia
T1  - Molecular diversity of autumn garlic genotypes using SSR markers
EP  - 228
SP  - 227
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2489
ER  - 

@conference{
author = "Glogovac, Svetlana and Gvozdanović-Varga, Jelica and Zorić, Miroslav and Nagl, Nevena and Trkulja, Dragana and Brbaklić, Ljiljana and Kondić-Špika, Ankica",
year = "2021",
abstract = "Garlic (Allium sativum L.) is one of the most important Allium species in terms of worldwide production and various usages in human nutrition, medicine, pharmacy and cosmetics. Basic method of garlic propagation is vegetative and creation of new varieties is mainly achieved by clonal selection. The characterization and preservation of samples in germplasm collections is of crucial importance in plant breeding, as well as availability of information about number and characteristics of samples in gene banks. Since pheotypic traits can vary significantly under the influence of environmental factors the characterization is more reliable by using DNA markers. Effective characterization of samples in collections and identification of duplicates is important from the economic aspect, ie. space saving and maintenance costs. The garlic collection of the Institute of Field and Vegetable Crops in Novi Sad (IFVCNS) includes 63 samples of autumn and 67 samples of spring garlic. These genotypes represent a valuable genetic pool for the selection of clones with appropriate characteristics, highly adapted for the production in the agro-climatic conditions of Serbia. Molecular characterization will provide more complete insight into diversity of samples, identification of potential duplicates and enable breeders more efficient selection and development of new cultivars.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Sciences",
journal = "Book of Abstracts, International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference, 25-26 November 2021, Novi Sad, Serbia",
title = "Molecular diversity of autumn garlic genotypes using SSR markers",
pages = "228-227",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2489"
}

Glogovac, S., Gvozdanović-Varga, J., Zorić, M., Nagl, N., Trkulja, D., Brbaklić, L.,& Kondić-Špika, A.. (2021). Molecular diversity of autumn garlic genotypes using SSR markers. in Book of Abstracts, International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference, 25-26 November 2021, Novi Sad, Serbia
Novi Sad : University of Novi Sad, Faculty of Sciences., 227-228.
https://hdl.handle.net/21.15107/rcub_fiver_2489

Glogovac S, Gvozdanović-Varga J, Zorić M, Nagl N, Trkulja D, Brbaklić L, Kondić-Špika A. Molecular diversity of autumn garlic genotypes using SSR markers. in Book of Abstracts, International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference, 25-26 November 2021, Novi Sad, Serbia. 2021;:227-228.
https://hdl.handle.net/21.15107/rcub_fiver_2489 .

Glogovac, Svetlana, Gvozdanović-Varga, Jelica, Zorić, Miroslav, Nagl, Nevena, Trkulja, Dragana, Brbaklić, Ljiljana, Kondić-Špika, Ankica, "Molecular diversity of autumn garlic genotypes using SSR markers" in Book of Abstracts, International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference, 25-26 November 2021, Novi Sad, Serbia (2021):227-228,
https://hdl.handle.net/21.15107/rcub_fiver_2489 .

TY  - PAT
AU  - Nagl, Nevena
AU  - Mitrović, Petar
AU  - Isakov, Milada
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3831
T1  - Alternaria brassicicola isolate F10 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702574.1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3831
ER  - 

@misc{
author = "Nagl, Nevena and Mitrović, Petar and Isakov, Milada",
year = "2021",
title = "Alternaria brassicicola isolate F10 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702574.1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3831"
}

Nagl, N., Mitrović, P.,& Isakov, M.. (2021). Alternaria brassicicola isolate F10 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702574.1. .
https://hdl.handle.net/21.15107/rcub_fiver_3831

Nagl N, Mitrović P, Isakov M. Alternaria brassicicola isolate F10 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702574.1. 2021;.
https://hdl.handle.net/21.15107/rcub_fiver_3831 .

Nagl, Nevena, Mitrović, Petar, Isakov, Milada, "Alternaria brassicicola isolate F10 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702574.1" (2021),
https://hdl.handle.net/21.15107/rcub_fiver_3831 .

TY  - PAT
AU  - Nagl, Nevena
AU  - Mitrović, Petar
AU  - Isakov, Milada
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3834
T1  - Alternaria brassicicola isolate F9 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702573.1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3834
ER  - 

@misc{
author = "Nagl, Nevena and Mitrović, Petar and Isakov, Milada",
year = "2021",
title = "Alternaria brassicicola isolate F9 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702573.1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3834"
}

Nagl, N., Mitrović, P.,& Isakov, M.. (2021). Alternaria brassicicola isolate F9 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702573.1. .
https://hdl.handle.net/21.15107/rcub_fiver_3834

Nagl N, Mitrović P, Isakov M. Alternaria brassicicola isolate F9 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702573.1. 2021;.
https://hdl.handle.net/21.15107/rcub_fiver_3834 .

Nagl, Nevena, Mitrović, Petar, Isakov, Milada, "Alternaria brassicicola isolate F9 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702573.1" (2021),
https://hdl.handle.net/21.15107/rcub_fiver_3834 .

TY  - PAT
AU  - Nagl, Nevena
AU  - Mitrović, Petar
AU  - Isakov, Milada
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3835
T1  - Alternaria brassicicola isolate F3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702572.1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3835
ER  - 

@misc{
author = "Nagl, Nevena and Mitrović, Petar and Isakov, Milada",
year = "2021",
title = "Alternaria brassicicola isolate F3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702572.1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3835"
}

Nagl, N., Mitrović, P.,& Isakov, M.. (2021). Alternaria brassicicola isolate F3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702572.1. .
https://hdl.handle.net/21.15107/rcub_fiver_3835

Nagl N, Mitrović P, Isakov M. Alternaria brassicicola isolate F3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702572.1. 2021;.
https://hdl.handle.net/21.15107/rcub_fiver_3835 .

Nagl, Nevena, Mitrović, Petar, Isakov, Milada, "Alternaria brassicicola isolate F3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702572.1" (2021),
https://hdl.handle.net/21.15107/rcub_fiver_3835 .

TY  - PAT
AU  - Nagl, Nevena
AU  - Mitrović, Petar
AU  - Isakov, Milada
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3836
T1  - Alternaria brassicicola isolate F2 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702571.1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3836
ER  - 

@misc{
author = "Nagl, Nevena and Mitrović, Petar and Isakov, Milada",
year = "2021",
title = "Alternaria brassicicola isolate F2 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702571.1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3836"
}

Nagl, N., Mitrović, P.,& Isakov, M.. (2021). Alternaria brassicicola isolate F2 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702571.1. .
https://hdl.handle.net/21.15107/rcub_fiver_3836

Nagl N, Mitrović P, Isakov M. Alternaria brassicicola isolate F2 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702571.1. 2021;.
https://hdl.handle.net/21.15107/rcub_fiver_3836 .

Nagl, Nevena, Mitrović, Petar, Isakov, Milada, "Alternaria brassicicola isolate F2 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ702571.1" (2021),
https://hdl.handle.net/21.15107/rcub_fiver_3836 .

TY  - PAT
AU  - Nagl, Nevena
AU  - Mitrović, Petar
AU  - Isakov, Milada
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3837
T1  - Alternaria brassicicola isolate M9   internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723932.1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3837
ER  - 

@misc{
author = "Nagl, Nevena and Mitrović, Petar and Isakov, Milada",
year = "2021",
title = "Alternaria brassicicola isolate M9   internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723932.1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3837"
}

Nagl, N., Mitrović, P.,& Isakov, M.. (2021). Alternaria brassicicola isolate M9   internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723932.1. .
https://hdl.handle.net/21.15107/rcub_fiver_3837

Nagl N, Mitrović P, Isakov M. Alternaria brassicicola isolate M9   internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723932.1. 2021;.
https://hdl.handle.net/21.15107/rcub_fiver_3837 .

Nagl, Nevena, Mitrović, Petar, Isakov, Milada, "Alternaria brassicicola isolate M9   internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723932.1" (2021),
https://hdl.handle.net/21.15107/rcub_fiver_3837 .

TY  - PAT
AU  - Nagl, Nevena
AU  - Mitrović, Petar
AU  - Isakov, Milada
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3832
T1  - Alternaria dauci isolate M7 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank: MZ723931.1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3832
ER  - 

@misc{
author = "Nagl, Nevena and Mitrović, Petar and Isakov, Milada",
year = "2021",
title = "Alternaria dauci isolate M7 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank: MZ723931.1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3832"
}

Nagl, N., Mitrović, P.,& Isakov, M.. (2021). Alternaria dauci isolate M7 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank: MZ723931.1. .
https://hdl.handle.net/21.15107/rcub_fiver_3832

Nagl N, Mitrović P, Isakov M. Alternaria dauci isolate M7 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank: MZ723931.1. 2021;.
https://hdl.handle.net/21.15107/rcub_fiver_3832 .

Nagl, Nevena, Mitrović, Petar, Isakov, Milada, "Alternaria dauci isolate M7 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank: MZ723931.1" (2021),
https://hdl.handle.net/21.15107/rcub_fiver_3832 .

TY  - PAT
AU  - Nagl, Nevena
AU  - Mitrović, Petar
AU  - Isakov, Milada
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3833
T1  - Alternaria dauci isolate M6 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723930.1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3833
ER  - 

@misc{
author = "Nagl, Nevena and Mitrović, Petar and Isakov, Milada",
year = "2021",
title = "Alternaria dauci isolate M6 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723930.1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3833"
}

Nagl, N., Mitrović, P.,& Isakov, M.. (2021). Alternaria dauci isolate M6 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723930.1. .
https://hdl.handle.net/21.15107/rcub_fiver_3833

Nagl N, Mitrović P, Isakov M. Alternaria dauci isolate M6 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723930.1. 2021;.
https://hdl.handle.net/21.15107/rcub_fiver_3833 .

Nagl, Nevena, Mitrović, Petar, Isakov, Milada, "Alternaria dauci isolate M6 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723930.1" (2021),
https://hdl.handle.net/21.15107/rcub_fiver_3833 .

TY  - PAT
AU  - Nagl, Nevena
AU  - Mitrović, Petar
AU  - Isakov, Milada
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3838
T1  - Alternaria dauci isolate M3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723929.1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3838
ER  - 

@misc{
author = "Nagl, Nevena and Mitrović, Petar and Isakov, Milada",
year = "2021",
title = "Alternaria dauci isolate M3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723929.1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3838"
}

Nagl, N., Mitrović, P.,& Isakov, M.. (2021). Alternaria dauci isolate M3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723929.1. .
https://hdl.handle.net/21.15107/rcub_fiver_3838

Nagl N, Mitrović P, Isakov M. Alternaria dauci isolate M3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723929.1. 2021;.
https://hdl.handle.net/21.15107/rcub_fiver_3838 .

Nagl, Nevena, Mitrović, Petar, Isakov, Milada, "Alternaria dauci isolate M3 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723929.1" (2021),
https://hdl.handle.net/21.15107/rcub_fiver_3838 .

TY  - PAT
AU  - Nagl, Nevena
AU  - Mitrović, Petar
AU  - Isakov, Milada
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3839
T1  - Alternaria dauci isolate M1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723928.1
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3839
ER  - 

@misc{
author = "Nagl, Nevena and Mitrović, Petar and Isakov, Milada",
year = "2021",
title = "Alternaria dauci isolate M1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723928.1",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3839"
}

Nagl, N., Mitrović, P.,& Isakov, M.. (2021). Alternaria dauci isolate M1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723928.1. .
https://hdl.handle.net/21.15107/rcub_fiver_3839

Nagl N, Mitrović P, Isakov M. Alternaria dauci isolate M1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723928.1. 2021;.
https://hdl.handle.net/21.15107/rcub_fiver_3839 .

Nagl, Nevena, Mitrović, Petar, Isakov, Milada, "Alternaria dauci isolate M1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. GenBank: MZ723928.1" (2021),
https://hdl.handle.net/21.15107/rcub_fiver_3839 .