TY  - JOUR
AU  - Vukotić, Jelena
AU  - Stojšin, Vera
AU  - Nagl, Nevena
AU  - Petreš, Mladen
AU  - Hrustić, jovana
AU  - Grahovac, Mila
AU  - Tanović, Boris
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3984
AB  - Neofabraea spp. is one of the major causal agents of the postharvest decay of apple fruit.
Bull’s eye rot can cause fruit rot, as well as tree canker. In Serbia, during February and March, 2017 and 2018, 56.3% of collected stored rotten apple fruits were found to be infected with Neofabraea spp. Using polymerase chain reaction, phylogenetic analysis, and morphological characterization, Neofabraea alba was identified as the causal agent of Bull’s eye rot. Twenty-five selected isolates were analyzed by DNA sequencing of the internal transcribed spacer and 16 S mitochondrial ribosomal RNA. The phylogenetic analyses provided information on the distance between Neofabraea spp., while merged phylogenetic tree was required to give insight and clarify the relationships between isolates of Neofabraea alba. N. alba demonstrated pathogenicity on apple fruit as well as on branches, with significantly different aggressiveness among the isolates. Isolates were able to produce larger cankers and acervuli on apple branches with abundant sporulation All identified isolates were separated into two morphotypes and described based on their macromorphological characteristics on culture media. It was noticed that different culture media, pH, and temperature could affect macromorphological characteristics and development. Representative isolates were able to grow at a temperature range of 0 to 25  C, above which only one isolate was able to grow on culture media. All isolates showed growth at a pH range of 2–10, with significant differences detected between isolates at different pH values. The type of cultivation medium significantly affected the size and shape of conidia, while the presence of the microconidia was recorded within isolates. The aim of this study was to identify the causal agents of Bull’s eye rot on stored apples in Serbia in the period 2017–2018 and characterize the obtained isolates based on pathogenic, morphological, physiological, and molecular criteria.
PB  - Basel : MDPI
T2  - Agronomy - Basel
T1  - Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia
IS  - 9
SP  - 2015
VL  - 12
DO  - 10.3390/agronomy12092015
ER  - 

@article{
author = "Vukotić, Jelena and Stojšin, Vera and Nagl, Nevena and Petreš, Mladen and Hrustić, jovana and Grahovac, Mila and Tanović, Boris",
year = "2022",
abstract = "Neofabraea spp. is one of the major causal agents of the postharvest decay of apple fruit.
Bull’s eye rot can cause fruit rot, as well as tree canker. In Serbia, during February and March, 2017 and 2018, 56.3% of collected stored rotten apple fruits were found to be infected with Neofabraea spp. Using polymerase chain reaction, phylogenetic analysis, and morphological characterization, Neofabraea alba was identified as the causal agent of Bull’s eye rot. Twenty-five selected isolates were analyzed by DNA sequencing of the internal transcribed spacer and 16 S mitochondrial ribosomal RNA. The phylogenetic analyses provided information on the distance between Neofabraea spp., while merged phylogenetic tree was required to give insight and clarify the relationships between isolates of Neofabraea alba. N. alba demonstrated pathogenicity on apple fruit as well as on branches, with significantly different aggressiveness among the isolates. Isolates were able to produce larger cankers and acervuli on apple branches with abundant sporulation All identified isolates were separated into two morphotypes and described based on their macromorphological characteristics on culture media. It was noticed that different culture media, pH, and temperature could affect macromorphological characteristics and development. Representative isolates were able to grow at a temperature range of 0 to 25  C, above which only one isolate was able to grow on culture media. All isolates showed growth at a pH range of 2–10, with significant differences detected between isolates at different pH values. The type of cultivation medium significantly affected the size and shape of conidia, while the presence of the microconidia was recorded within isolates. The aim of this study was to identify the causal agents of Bull’s eye rot on stored apples in Serbia in the period 2017–2018 and characterize the obtained isolates based on pathogenic, morphological, physiological, and molecular criteria.",
publisher = "Basel : MDPI",
journal = "Agronomy - Basel",
title = "Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia",
number = "9",
pages = "2015",
volume = "12",
doi = "10.3390/agronomy12092015"
}

Vukotić, J., Stojšin, V., Nagl, N., Petreš, M., Hrustić, j., Grahovac, M.,& Tanović, B.. (2022). Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia. in Agronomy - Basel
Basel : MDPI., 12(9), 2015.
https://doi.org/10.3390/agronomy12092015

Vukotić J, Stojšin V, Nagl N, Petreš M, Hrustić J, Grahovac M, Tanović B. Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia. in Agronomy - Basel. 2022;12(9):2015.
doi:10.3390/agronomy12092015 .

Vukotić, Jelena, Stojšin, Vera, Nagl, Nevena, Petreš, Mladen, Hrustić, jovana, Grahovac, Mila, Tanović, Boris, "Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia" in Agronomy - Basel, 12, no. 9 (2022):2015,
https://doi.org/10.3390/agronomy12092015 . .

TY  - JOUR
AU  - Savić, Zagorka
AU  - Dudaš, Tatjana
AU  - Loc, Marta
AU  - Grahovac, Mila
AU  - Budakov, Dragana
AU  - Jajić, Igor
AU  - Krstović, Saša
AU  - Barošević, Tijana
AU  - Krska, Rudolf
AU  - Sulyok, Michael
AU  - Stojšin, Vera
AU  - Petreš, Mladen
AU  - Stankov, Aleksandra
AU  - Vukotić, Jelena
AU  - Bagi, Ferenc
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/4355
AB  - Aspergillus flavus is the main producer of aflatoxin B1, one of the most toxic contaminants
of food and feed. With global warming, climate conditions have become favourable for aflatoxin
contamination of agricultural products in several European countries, including Serbia. The
infection of maize with A. flavus, and aflatoxin synthesis can be controlled and reduced by
application of a biocontrol product based on non‐toxigenic strains of A. flavus. Biological control
relies on competition between atoxigenic and toxigenic strains. This is the most commonly used
biological control mechanism of aflatoxin contamination in maize in countries where aflatoxins
pose a significant threat. Mytoolbox Af01, a native atoxigenic A. flavus strain, was obtained from
maize grown in Serbia and used to produce a biocontrol product that was applied in irrigated and
non‐irrigated Serbian fields during 2016 and 2017. The application of this biocontrol product
reduced aflatoxin levels in maize kernels (51–83%). The biocontrol treatment had a highly
significant effect of reducing total aflatoxin contamination by 73%. This study showed that
aflatoxin contamination control in Serbian maize can be achieved through biological control
methods using atoxigenic A. flavus strains.
PB  - Basel : MDPI
T2  - Toxins
T1  - Biological Control of Aflatoxin in Maize Grown in Serbia
IS  - 3
SP  - 162
VL  - 12
DO  - 10.3390/toxins12030162
ER  - 

@article{
author = "Savić, Zagorka and Dudaš, Tatjana and Loc, Marta and Grahovac, Mila and Budakov, Dragana and Jajić, Igor and Krstović, Saša and Barošević, Tijana and Krska, Rudolf and Sulyok, Michael and Stojšin, Vera and Petreš, Mladen and Stankov, Aleksandra and Vukotić, Jelena and Bagi, Ferenc",
year = "2020",
abstract = "Aspergillus flavus is the main producer of aflatoxin B1, one of the most toxic contaminants
of food and feed. With global warming, climate conditions have become favourable for aflatoxin
contamination of agricultural products in several European countries, including Serbia. The
infection of maize with A. flavus, and aflatoxin synthesis can be controlled and reduced by
application of a biocontrol product based on non‐toxigenic strains of A. flavus. Biological control
relies on competition between atoxigenic and toxigenic strains. This is the most commonly used
biological control mechanism of aflatoxin contamination in maize in countries where aflatoxins
pose a significant threat. Mytoolbox Af01, a native atoxigenic A. flavus strain, was obtained from
maize grown in Serbia and used to produce a biocontrol product that was applied in irrigated and
non‐irrigated Serbian fields during 2016 and 2017. The application of this biocontrol product
reduced aflatoxin levels in maize kernels (51–83%). The biocontrol treatment had a highly
significant effect of reducing total aflatoxin contamination by 73%. This study showed that
aflatoxin contamination control in Serbian maize can be achieved through biological control
methods using atoxigenic A. flavus strains.",
publisher = "Basel : MDPI",
journal = "Toxins",
title = "Biological Control of Aflatoxin in Maize Grown in Serbia",
number = "3",
pages = "162",
volume = "12",
doi = "10.3390/toxins12030162"
}

Savić, Z., Dudaš, T., Loc, M., Grahovac, M., Budakov, D., Jajić, I., Krstović, S., Barošević, T., Krska, R., Sulyok, M., Stojšin, V., Petreš, M., Stankov, A., Vukotić, J.,& Bagi, F.. (2020). Biological Control of Aflatoxin in Maize Grown in Serbia. in Toxins
Basel : MDPI., 12(3), 162.
https://doi.org/10.3390/toxins12030162

Savić Z, Dudaš T, Loc M, Grahovac M, Budakov D, Jajić I, Krstović S, Barošević T, Krska R, Sulyok M, Stojšin V, Petreš M, Stankov A, Vukotić J, Bagi F. Biological Control of Aflatoxin in Maize Grown in Serbia. in Toxins. 2020;12(3):162.
doi:10.3390/toxins12030162 .

Savić, Zagorka, Dudaš, Tatjana, Loc, Marta, Grahovac, Mila, Budakov, Dragana, Jajić, Igor, Krstović, Saša, Barošević, Tijana, Krska, Rudolf, Sulyok, Michael, Stojšin, Vera, Petreš, Mladen, Stankov, Aleksandra, Vukotić, Jelena, Bagi, Ferenc, "Biological Control of Aflatoxin in Maize Grown in Serbia" in Toxins, 12, no. 3 (2020):162,
https://doi.org/10.3390/toxins12030162 . .

TY  - JOUR
AU  - Meseldžija, Maja
AU  - Lazić, Sanja
AU  - Dudić, Milica
AU  - Šunjka, Dragana B.
AU  - Rajković, Miloš
AU  - Marković, Todor
AU  - Vukotić, Jelena
AU  - Ljevnaić-Mašić, Branka
AU  - Jurišić, Aleksandar
AU  - Ivanović, Ivana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2050
AB  - Sub-lethal doses of herbicides can promote plant growth and have a positive effect on an organism this is called hormesis. The purpose of this study was to test the effects of sub-lethal doses of glyphosate on soybean (Glycine max(L.) Merr.) (1.8, 3.6, 7.2, 36, 180, and 720 g ha(-1)) and Amaranthus retroflexus L. (7.2, 36, 180, 720, 1440, and 2880 g ha(-1)). Different biological parameters, such as phytotoxicity, fresh weight, root length, content of photosynthetic pigments, and shikimate concentration, were measured. Glyphosate in doses of 1440 and 2880 g ha(-1) destroyed A. retroflexus plants. A fresh weight of A. retroflexus at a dose of 36 g ha(-1) was reduced by 76.31%, while for the soybean it was reduced by 19.26%. At the highest dose, the shikimate concentration was 145% in the soybean, while in A. retroflexus, the concentration increased by 58.80% compared to the control plants. All doses of glyphosate were statistically significantly different in terms of chlorophyll a content, while higher doses in A. retroflexus caused chlorophyll b to decrease. The change in the production of carotenoids was not statistically significant. The results showed that sub-lethal amounts of glyphosate did not lead to stimulation of measured parameters of soybean.
PB  - Basel : MDPI
T2  - Agronomy-Basel
T1  - Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?
IS  - 6
VL  - 10
DO  - 10.3390/agronomy10060850
ER  - 

@article{
author = "Meseldžija, Maja and Lazić, Sanja and Dudić, Milica and Šunjka, Dragana B. and Rajković, Miloš and Marković, Todor and Vukotić, Jelena and Ljevnaić-Mašić, Branka and Jurišić, Aleksandar and Ivanović, Ivana",
year = "2020",
abstract = "Sub-lethal doses of herbicides can promote plant growth and have a positive effect on an organism this is called hormesis. The purpose of this study was to test the effects of sub-lethal doses of glyphosate on soybean (Glycine max(L.) Merr.) (1.8, 3.6, 7.2, 36, 180, and 720 g ha(-1)) and Amaranthus retroflexus L. (7.2, 36, 180, 720, 1440, and 2880 g ha(-1)). Different biological parameters, such as phytotoxicity, fresh weight, root length, content of photosynthetic pigments, and shikimate concentration, were measured. Glyphosate in doses of 1440 and 2880 g ha(-1) destroyed A. retroflexus plants. A fresh weight of A. retroflexus at a dose of 36 g ha(-1) was reduced by 76.31%, while for the soybean it was reduced by 19.26%. At the highest dose, the shikimate concentration was 145% in the soybean, while in A. retroflexus, the concentration increased by 58.80% compared to the control plants. All doses of glyphosate were statistically significantly different in terms of chlorophyll a content, while higher doses in A. retroflexus caused chlorophyll b to decrease. The change in the production of carotenoids was not statistically significant. The results showed that sub-lethal amounts of glyphosate did not lead to stimulation of measured parameters of soybean.",
publisher = "Basel : MDPI",
journal = "Agronomy-Basel",
title = "Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?",
number = "6",
volume = "10",
doi = "10.3390/agronomy10060850"
}

Meseldžija, M., Lazić, S., Dudić, M., Šunjka, D. B., Rajković, M., Marković, T., Vukotić, J., Ljevnaić-Mašić, B., Jurišić, A.,& Ivanović, I.. (2020). Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?. in Agronomy-Basel
Basel : MDPI., 10(6).
https://doi.org/10.3390/agronomy10060850

Meseldžija M, Lazić S, Dudić M, Šunjka DB, Rajković M, Marković T, Vukotić J, Ljevnaić-Mašić B, Jurišić A, Ivanović I. Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?. in Agronomy-Basel. 2020;10(6).
doi:10.3390/agronomy10060850 .

Meseldžija, Maja, Lazić, Sanja, Dudić, Milica, Šunjka, Dragana B., Rajković, Miloš, Marković, Todor, Vukotić, Jelena, Ljevnaić-Mašić, Branka, Jurišić, Aleksandar, Ivanović, Ivana, "Is There a Possibility to Involve the Hormesis Effect on the Soybean with Glyphosate Sub-Lethal Amounts Used to Control Weed Species Amaranthus retroflexus L.?" in Agronomy-Basel, 10, no. 6 (2020),
https://doi.org/10.3390/agronomy10060850 . .