TY  - CONF
AU  - Stankov Petreš, Aleksandra
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Nagl, Nevena
AU  - Grahovac, Mila
AU  - Ćurčić, Živko
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3368
AB  - Primena fungicida u suzbijanju zemljišnih patogena ne predstavlja efikasnu i ekonomski opravdanu meru. Takođe, oni mogu da naruše balans korisnih mikroorganizama u zemljištu, dok njihove rezidue utiču na zagađenje životne sredine. U nastojanju da se smanji upotreba sintetičkih fungicida, primena bioloških agenasa može imati važnu ulogu u „eco-frendly“ poljoprivrednim sistemima. Biološki agensi kao što su vrste iz rodova Bacillus i Trichoderma se najčešće upotrebljavaju u biološkoj borbi protiv zemljišnih patogena. Cilj ovog istraživanja je bio da se ispita antagonistički uticaj Bacillus sp. i Trichoderma spp. na izolate Macrophomina phaseolina (ŠR55(3)/09 i ŠR23/11) u in vitro uslovima. Za ispitivanje je korišten Bacillus sp., 74 koji je komercijalno dostupan kao mikrobiološko đubrivo pod nazivom Bacillomix® Original (Bacillomix Co., Srbija). Metodom inkorporacije komercijalne suspenzije mikrobiološkog đubriva u KDA hranljivu podlogu ispitane su koncentracije preparata od 1, 2, 5 i 10%. U cilju ispitivanja osetljivosti M. phaseolina prema Trichoderma spp. korišteno je 13 izolata koji su predhodno identifikovani kao Trichoderma harzianum. Test dvojne kulture (TDK) je korišten kako bi utvrdili antagonistički uticaj ispitivanih izolata.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.
T1  - Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro
EP  - 74
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3368
ER  - 

@conference{
author = "Stankov Petreš, Aleksandra and Budakov, Dragana and Stojšin, Vera and Nagl, Nevena and Grahovac, Mila and Ćurčić, Živko",
year = "2022",
abstract = "Primena fungicida u suzbijanju zemljišnih patogena ne predstavlja efikasnu i ekonomski opravdanu meru. Takođe, oni mogu da naruše balans korisnih mikroorganizama u zemljištu, dok njihove rezidue utiču na zagađenje životne sredine. U nastojanju da se smanji upotreba sintetičkih fungicida, primena bioloških agenasa može imati važnu ulogu u „eco-frendly“ poljoprivrednim sistemima. Biološki agensi kao što su vrste iz rodova Bacillus i Trichoderma se najčešće upotrebljavaju u biološkoj borbi protiv zemljišnih patogena. Cilj ovog istraživanja je bio da se ispita antagonistički uticaj Bacillus sp. i Trichoderma spp. na izolate Macrophomina phaseolina (ŠR55(3)/09 i ŠR23/11) u in vitro uslovima. Za ispitivanje je korišten Bacillus sp., 74 koji je komercijalno dostupan kao mikrobiološko đubrivo pod nazivom Bacillomix® Original (Bacillomix Co., Srbija). Metodom inkorporacije komercijalne suspenzije mikrobiološkog đubriva u KDA hranljivu podlogu ispitane su koncentracije preparata od 1, 2, 5 i 10%. U cilju ispitivanja osetljivosti M. phaseolina prema Trichoderma spp. korišteno je 13 izolata koji su predhodno identifikovani kao Trichoderma harzianum. Test dvojne kulture (TDK) je korišten kako bi utvrdili antagonistički uticaj ispitivanih izolata.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.",
title = "Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro",
pages = "74-73",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3368"
}

Stankov Petreš, A., Budakov, D., Stojšin, V., Nagl, N., Grahovac, M.,& Ćurčić, Ž.. (2022). Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro. in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.
Beograd : Društvo za zaštitu bilja Srbije., 73-74.
https://hdl.handle.net/21.15107/rcub_fiver_3368

Stankov Petreš A, Budakov D, Stojšin V, Nagl N, Grahovac M, Ćurčić Ž. Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro. in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.. 2022;:73-74.
https://hdl.handle.net/21.15107/rcub_fiver_3368 .

Stankov Petreš, Aleksandra, Budakov, Dragana, Stojšin, Vera, Nagl, Nevena, Grahovac, Mila, Ćurčić, Živko, "Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro" in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022. (2022):73-74,
https://hdl.handle.net/21.15107/rcub_fiver_3368 .

TY  - CONF
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Petreš, Mladen
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Grahovac, Mila
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2432
AB  - Vrste Pectobacterium carotovorum subsp. brasiliense (Pcb) i Pectobacterium carotovorum subsp. carotovorum (Pcc) su tokom 2018. i 2019. godine na terioriji AP Vojvodine registrovane kao dominanatni prouzrokovači crne truleži prizemnog dela stabla i vlažne truleži krtola krompira. U postupku detekcije i identifikacije ovih prouzrokovača na simptomatičnim biljakama krompira tokom 2018. i 2019. godine, sekvenciranjem 16S rRNK regiona je za čak 90% dobijenih izolata potvrđena pripadnost vrsti Pcb. Godišnji gubici prinosa i kvaliteta krompira, usled delovanja ovih vrsta poprimaju sve veće razmere, te se one svrstavaju među deset ekonomski najznačajnijih fitopatogenih bakterija. Pored šteta koje nastaju u toku vegetacije, za vreme čuvanja krtola krompira u skladištu pa do njegove upotrebe, patogeni nastavljaju svoju aktivnost, te celokupne partije uskladištenih krtola mogu veoma brzo propasti. Najznačajniji izvor infekcije predstavljaju zaražene semenske krtole, te patogeni ovim putem imaju mogućnost lakog dospevanja u nova područja.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
T1  - Patogenost Pectobacterium spp. na krtolama različitih sorti krompira
EP  - 47
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2432
ER  - 

@conference{
author = "Loc, Marta and Milošević, Dragana and Ignjatov, Maja and Petreš, Mladen and Budakov, Dragana and Stojšin, Vera and Grahovac, Mila",
year = "2021",
abstract = "Vrste Pectobacterium carotovorum subsp. brasiliense (Pcb) i Pectobacterium carotovorum subsp. carotovorum (Pcc) su tokom 2018. i 2019. godine na terioriji AP Vojvodine registrovane kao dominanatni prouzrokovači crne truleži prizemnog dela stabla i vlažne truleži krtola krompira. U postupku detekcije i identifikacije ovih prouzrokovača na simptomatičnim biljakama krompira tokom 2018. i 2019. godine, sekvenciranjem 16S rRNK regiona je za čak 90% dobijenih izolata potvrđena pripadnost vrsti Pcb. Godišnji gubici prinosa i kvaliteta krompira, usled delovanja ovih vrsta poprimaju sve veće razmere, te se one svrstavaju među deset ekonomski najznačajnijih fitopatogenih bakterija. Pored šteta koje nastaju u toku vegetacije, za vreme čuvanja krtola krompira u skladištu pa do njegove upotrebe, patogeni nastavljaju svoju aktivnost, te celokupne partije uskladištenih krtola mogu veoma brzo propasti. Najznačajniji izvor infekcije predstavljaju zaražene semenske krtole, te patogeni ovim putem imaju mogućnost lakog dospevanja u nova područja.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.",
title = "Patogenost Pectobacterium spp. na krtolama različitih sorti krompira",
pages = "47-46",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2432"
}

Loc, M., Milošević, D., Ignjatov, M., Petreš, M., Budakov, D., Stojšin, V.,& Grahovac, M.. (2021). Patogenost Pectobacterium spp. na krtolama različitih sorti krompira. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
Beograd : Društvo za zaštitu bilja Srbije., 46-47.
https://hdl.handle.net/21.15107/rcub_fiver_2432

Loc M, Milošević D, Ignjatov M, Petreš M, Budakov D, Stojšin V, Grahovac M. Patogenost Pectobacterium spp. na krtolama različitih sorti krompira. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.. 2021;:46-47.
https://hdl.handle.net/21.15107/rcub_fiver_2432 .

Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Petreš, Mladen, Budakov, Dragana, Stojšin, Vera, Grahovac, Mila, "Patogenost Pectobacterium spp. na krtolama različitih sorti krompira" in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. (2021):46-47,
https://hdl.handle.net/21.15107/rcub_fiver_2432 .

TY  - CONF
AU  - Petreš, Mladen
AU  - Aćimović, Milica
AU  - Tešević, Vele
AU  - Loc, Marta
AU  - Stojšin, Vera
AU  - Budakov, Dragana
AU  - Grahovac, Mila
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2431
AB  - Fitopatogene gljive značajno ugrožavaju kvalitet plodova jabuke tokom skladištenja. Vrste Colletotrichum acutatum i Colletotrichum gloeosporioides, prouzrokovači gorke truleži (antraknoze) plodova jabuke, u pojedinim godinama prouzrokuju značajne gubitke. Colletotrichum spp. zarazu plodova jabuke ostvaruju pre berbe plodova, ali je zaraza moguća i posle ovog perioda. Zaštita plodova jabuke od prouzrokovača truleži se u najvećoj meri oslanja na primenu sintetičkih fungicida, pre berbe plodova. Zbog nepovoljnih ekotoksikoloških svojstava i pojave rezistentnosti, primena hemijskih fungicida tokom vegetacionog perioda se sve više redukuje, dok je njihova upotreba posle berbe plodova zabranjena u Evropskoj uniji, ali i u Srbiji. Imajući u vidu navedeno, kao i činjenicu da su zahtevi potrošača za zdravstveno bezbednom hranom sve izraženiji, istraživanja o alternativnim načinima borbe protiv patogena uskladištenih plodova voća sve više dobijaju na značaju. Etarska ulja pojedinih biljaka ispoljavaju snažno antifungalno dejstvo, te mogu biti potencijalni agensi zaštite plodova jabuke od prouzrokovača truleži.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
T1  - Uticaj etarskih ulja na Colletotrichum spp. – prouzrokovače gorke truleži jabuke
EP  - 44
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2431
ER  - 

@conference{
author = "Petreš, Mladen and Aćimović, Milica and Tešević, Vele and Loc, Marta and Stojšin, Vera and Budakov, Dragana and Grahovac, Mila",
year = "2021",
abstract = "Fitopatogene gljive značajno ugrožavaju kvalitet plodova jabuke tokom skladištenja. Vrste Colletotrichum acutatum i Colletotrichum gloeosporioides, prouzrokovači gorke truleži (antraknoze) plodova jabuke, u pojedinim godinama prouzrokuju značajne gubitke. Colletotrichum spp. zarazu plodova jabuke ostvaruju pre berbe plodova, ali je zaraza moguća i posle ovog perioda. Zaštita plodova jabuke od prouzrokovača truleži se u najvećoj meri oslanja na primenu sintetičkih fungicida, pre berbe plodova. Zbog nepovoljnih ekotoksikoloških svojstava i pojave rezistentnosti, primena hemijskih fungicida tokom vegetacionog perioda se sve više redukuje, dok je njihova upotreba posle berbe plodova zabranjena u Evropskoj uniji, ali i u Srbiji. Imajući u vidu navedeno, kao i činjenicu da su zahtevi potrošača za zdravstveno bezbednom hranom sve izraženiji, istraživanja o alternativnim načinima borbe protiv patogena uskladištenih plodova voća sve više dobijaju na značaju. Etarska ulja pojedinih biljaka ispoljavaju snažno antifungalno dejstvo, te mogu biti potencijalni agensi zaštite plodova jabuke od prouzrokovača truleži.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.",
title = "Uticaj etarskih ulja na Colletotrichum spp. – prouzrokovače gorke truleži jabuke",
pages = "44-43",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2431"
}

Petreš, M., Aćimović, M., Tešević, V., Loc, M., Stojšin, V., Budakov, D.,& Grahovac, M.. (2021). Uticaj etarskih ulja na Colletotrichum spp. – prouzrokovače gorke truleži jabuke. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
Beograd : Društvo za zaštitu bilja Srbije., 43-44.
https://hdl.handle.net/21.15107/rcub_fiver_2431

Petreš M, Aćimović M, Tešević V, Loc M, Stojšin V, Budakov D, Grahovac M. Uticaj etarskih ulja na Colletotrichum spp. – prouzrokovače gorke truleži jabuke. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.. 2021;:43-44.
https://hdl.handle.net/21.15107/rcub_fiver_2431 .

Petreš, Mladen, Aćimović, Milica, Tešević, Vele, Loc, Marta, Stojšin, Vera, Budakov, Dragana, Grahovac, Mila, "Uticaj etarskih ulja na Colletotrichum spp. – prouzrokovače gorke truleži jabuke" in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. (2021):43-44,
https://hdl.handle.net/21.15107/rcub_fiver_2431 .

TY  - CONF
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Ćurčić, Živko
AU  - Dudaš, Tatjana
AU  - Stankov, Aleksandra
AU  - Bagi, Ferenc
AU  - Grahovac, Mila
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/4252
AB  - Cercospora beticola is economically the most important sugar beet leaf disease primarily controlled by fungicide applications. However, other factors influence its incidence as well, some of which are the choice of cultivar and plant nutrition. These factors have been put together in field trial, which tested reaction of eight sugar beet genotypes (G1-G8) and 20 variants of NPK nutrition.
PB  - International Institute of Sugar Beet Research
C3  - Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels
T1  - Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot
EP  - 72
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4252
ER  - 

@conference{
author = "Budakov, Dragana and Stojšin, Vera and Ćurčić, Živko and Dudaš, Tatjana and Stankov, Aleksandra and Bagi, Ferenc and Grahovac, Mila",
year = "2020",
abstract = "Cercospora beticola is economically the most important sugar beet leaf disease primarily controlled by fungicide applications. However, other factors influence its incidence as well, some of which are the choice of cultivar and plant nutrition. These factors have been put together in field trial, which tested reaction of eight sugar beet genotypes (G1-G8) and 20 variants of NPK nutrition.",
publisher = "International Institute of Sugar Beet Research",
journal = "Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels",
title = "Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot",
pages = "72-72",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4252"
}

Budakov, D., Stojšin, V., Ćurčić, Ž., Dudaš, T., Stankov, A., Bagi, F.,& Grahovac, M.. (2020). Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot. in Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels
International Institute of Sugar Beet Research., 72-72.
https://hdl.handle.net/21.15107/rcub_fiver_4252

Budakov D, Stojšin V, Ćurčić Ž, Dudaš T, Stankov A, Bagi F, Grahovac M. Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot. in Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels. 2020;:72-72.
https://hdl.handle.net/21.15107/rcub_fiver_4252 .

Budakov, Dragana, Stojšin, Vera, Ćurčić, Živko, Dudaš, Tatjana, Stankov, Aleksandra, Bagi, Ferenc, Grahovac, Mila, "Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot" in Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels (2020):72-72,
https://hdl.handle.net/21.15107/rcub_fiver_4252 .

TY  - JOUR
AU  - Loc, Marta
AU  - Petrović, Gordana
AU  - Ignjatov, Maja
AU  - Budakov, Dragana
AU  - Petreš, Mladen
AU  - Stojšin, Vera
AU  - Grahovac, Mila
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1960
AB  - World potato production is affected by plant pathogenic bacteria, which significantly limit crop yield and quality in recent years. Bacteria belonging to Pectobacterium and Dickeya genera, causal agents of blackleg and tuber soft rot of potato, are considered as one of the most important pathogens that affect plant production worldwide. Main source of infection are latently infected seed tubers, which transmitted over long trade distances significantly increase disease incidence. Main identification tools for Pectobacterium and Dickeya species until recently were morphological and biochemical characterization methods, followed by pathogenicity tests. Due to phylogenetic heterogeneity of the soft rot and blackleg causing bacteria, identification and differentiation to species and subspecies level became increasingly difficult. Therefore, development of efficient and reliable identification methods is of high importance. Molecular and serological methods offer accurate, reliable and cost-effective differentiation of high specificity and reproducibility. This work aimed to review Pectobacterium i Dickeya species occurring on potato, with special highlight on available molecular identification techniques.
AB  - Proizvodnja krompira u svetu značajno je ugrožena delovanjem fitopatogenih bakterija, te godišnji gubici prinosa poprimaju sve veće razmere. Među deset ekonomski najznačajnijih fitopatogenih bakterija koje smanjuju prinos i kvalitet poljoprivrednih kultura ističu se vrste rodova Pectobacterium i Dickeya - prouzrokovači crne truleži prizemnog dela stabla "crna noga" i vlažne truleži krtola krompira. Najznačajniji izvor infekcije su zaražene semenske krtole, te bakterije ovim putem lako dospevaju u nova područja. Identifikacija bakterija rodova Pectobacterium i Dickeya se do nedavno zasnivala na proučavanju morfoloških odlika bakterijskih kolonija uz upotpunjavanje testovima patogenosti i biohemijskim analizama. Zbog heterogenosti populacije patogena precizna identifikacija prouzrokovača vlažne truleži je dodatno otežana. S obzirom na potrebe za brzom i pouzdanom identifikacijom, u primenu su uvedene serološke i molekularne metode. Molekularne tehnike identifikacije, zbog svoje visoke specifičnosti, brzine izvođenja, pouzdanosti i ponovljivosti danas zauzimaju vodeće mesto u identifikaciji patogena. U radu su prikazane vrste rodova Pectobacterium i Dickeya koje se javljaju na krompiru, sa posebnim osvrtom na molekularne tehnike identifikacije.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot
T1  - Vrste rodova Pectobacterium i Dickeya - prouzrokovači crne truleži prizemnog dela stabla i vlažne truleži krtola krompira
EP  - 66
IS  - 1
SP  - 52
VL  - 48
DO  - 10.5937/BiljLek2001052L
ER  - 

@article{
author = "Loc, Marta and Petrović, Gordana and Ignjatov, Maja and Budakov, Dragana and Petreš, Mladen and Stojšin, Vera and Grahovac, Mila",
year = "2020",
abstract = "World potato production is affected by plant pathogenic bacteria, which significantly limit crop yield and quality in recent years. Bacteria belonging to Pectobacterium and Dickeya genera, causal agents of blackleg and tuber soft rot of potato, are considered as one of the most important pathogens that affect plant production worldwide. Main source of infection are latently infected seed tubers, which transmitted over long trade distances significantly increase disease incidence. Main identification tools for Pectobacterium and Dickeya species until recently were morphological and biochemical characterization methods, followed by pathogenicity tests. Due to phylogenetic heterogeneity of the soft rot and blackleg causing bacteria, identification and differentiation to species and subspecies level became increasingly difficult. Therefore, development of efficient and reliable identification methods is of high importance. Molecular and serological methods offer accurate, reliable and cost-effective differentiation of high specificity and reproducibility. This work aimed to review Pectobacterium i Dickeya species occurring on potato, with special highlight on available molecular identification techniques., Proizvodnja krompira u svetu značajno je ugrožena delovanjem fitopatogenih bakterija, te godišnji gubici prinosa poprimaju sve veće razmere. Među deset ekonomski najznačajnijih fitopatogenih bakterija koje smanjuju prinos i kvalitet poljoprivrednih kultura ističu se vrste rodova Pectobacterium i Dickeya - prouzrokovači crne truleži prizemnog dela stabla "crna noga" i vlažne truleži krtola krompira. Najznačajniji izvor infekcije su zaražene semenske krtole, te bakterije ovim putem lako dospevaju u nova područja. Identifikacija bakterija rodova Pectobacterium i Dickeya se do nedavno zasnivala na proučavanju morfoloških odlika bakterijskih kolonija uz upotpunjavanje testovima patogenosti i biohemijskim analizama. Zbog heterogenosti populacije patogena precizna identifikacija prouzrokovača vlažne truleži je dodatno otežana. S obzirom na potrebe za brzom i pouzdanom identifikacijom, u primenu su uvedene serološke i molekularne metode. Molekularne tehnike identifikacije, zbog svoje visoke specifičnosti, brzine izvođenja, pouzdanosti i ponovljivosti danas zauzimaju vodeće mesto u identifikaciji patogena. U radu su prikazane vrste rodova Pectobacterium i Dickeya koje se javljaju na krompiru, sa posebnim osvrtom na molekularne tehnike identifikacije.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot, Vrste rodova Pectobacterium i Dickeya - prouzrokovači crne truleži prizemnog dela stabla i vlažne truleži krtola krompira",
pages = "66-52",
number = "1",
volume = "48",
doi = "10.5937/BiljLek2001052L"
}

Loc, M., Petrović, G., Ignjatov, M., Budakov, D., Petreš, M., Stojšin, V.,& Grahovac, M.. (2020). Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 48(1), 52-66.
https://doi.org/10.5937/BiljLek2001052L

Loc M, Petrović G, Ignjatov M, Budakov D, Petreš M, Stojšin V, Grahovac M. Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot. in Biljni lekar. 2020;48(1):52-66.
doi:10.5937/BiljLek2001052L .

Loc, Marta, Petrović, Gordana, Ignjatov, Maja, Budakov, Dragana, Petreš, Mladen, Stojšin, Vera, Grahovac, Mila, "Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot" in Biljni lekar, 48, no. 1 (2020):52-66,
https://doi.org/10.5937/BiljLek2001052L . .

TY  - JOUR
AU  - Szabo, Balazs
AU  - Toth, Beata
AU  - Toldine, Eva Toth
AU  - Varga, Monika
AU  - Kovacs, Nandor
AU  - Varga, Janos
AU  - Kocsube, Sandor
AU  - Palagyi, Andrea
AU  - Bagi, Ferenc
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Lazić, Sanja
AU  - Bodroža-Solarov, Marija
AU  - Čolović, Radmilo
AU  - Bekavac, Goran
AU  - Purar, Božana
AU  - Jocković, Đorđe
AU  - Mesterhazy, Akos
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1752
AB  - Commercial maize hybrids are exposed to different degrees of ear infection by toxigenic fungal species and toxin contamination. Their resistance to different fungi and toxin relationships are largely unknown. Without this knowledge, screening and breeding are not possible for these pathogens. Seven- to tenfold differences were found in resistance to Fusarium spp., and there was a five-fold difference in ear coverage (%) in response to A. flavus. Three hybrids of the twenty entries had lower infection severity compared with the general means for toxigenic species. Three were highly susceptible to each, and 14 hybrids reacted differently to the different fungi. Differences were also observed in the toxin content. Again, three hybrids had lower toxin content in response to all toxigenic species, one had higher values for all, and 16 had variable resistance levels. Correlations between infection severity and deoxynivalenol (DON) content were 0.95 and 0.82 (p = 0.001) for F. graminearum and F. culmorum, respectively. For fumonisin and F. verticillioides ear rot, the Pearson correlation coefficient (r) was 0.45 (p = 0.05). Two independent isolates with different aggressiveness were used, and their mean X values better described the resistance levels. This increased the reliability of the data. With the introduction of this methodological concept (testing the resistance levels separately for different fungi and with two isolates independently), highly significant resistance differences were found. The resistance to different fungal species correlated only in certain cases; thus, each should be tested separately. This is very useful in registration tests and post-registration screening and breeding. This would allow a rapid increase in food and feed safety.
PB  - Basel : MDPI
T2  - Toxins
T1  - A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize
IS  - 9
VL  - 10
DO  - 10.3390/toxins10090372
ER  - 

@article{
author = "Szabo, Balazs and Toth, Beata and Toldine, Eva Toth and Varga, Monika and Kovacs, Nandor and Varga, Janos and Kocsube, Sandor and Palagyi, Andrea and Bagi, Ferenc and Budakov, Dragana and Stojšin, Vera and Lazić, Sanja and Bodroža-Solarov, Marija and Čolović, Radmilo and Bekavac, Goran and Purar, Božana and Jocković, Đorđe and Mesterhazy, Akos",
year = "2018",
abstract = "Commercial maize hybrids are exposed to different degrees of ear infection by toxigenic fungal species and toxin contamination. Their resistance to different fungi and toxin relationships are largely unknown. Without this knowledge, screening and breeding are not possible for these pathogens. Seven- to tenfold differences were found in resistance to Fusarium spp., and there was a five-fold difference in ear coverage (%) in response to A. flavus. Three hybrids of the twenty entries had lower infection severity compared with the general means for toxigenic species. Three were highly susceptible to each, and 14 hybrids reacted differently to the different fungi. Differences were also observed in the toxin content. Again, three hybrids had lower toxin content in response to all toxigenic species, one had higher values for all, and 16 had variable resistance levels. Correlations between infection severity and deoxynivalenol (DON) content were 0.95 and 0.82 (p = 0.001) for F. graminearum and F. culmorum, respectively. For fumonisin and F. verticillioides ear rot, the Pearson correlation coefficient (r) was 0.45 (p = 0.05). Two independent isolates with different aggressiveness were used, and their mean X values better described the resistance levels. This increased the reliability of the data. With the introduction of this methodological concept (testing the resistance levels separately for different fungi and with two isolates independently), highly significant resistance differences were found. The resistance to different fungal species correlated only in certain cases; thus, each should be tested separately. This is very useful in registration tests and post-registration screening and breeding. This would allow a rapid increase in food and feed safety.",
publisher = "Basel : MDPI",
journal = "Toxins",
title = "A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize",
number = "9",
volume = "10",
doi = "10.3390/toxins10090372"
}

Szabo, B., Toth, B., Toldine, E. T., Varga, M., Kovacs, N., Varga, J., Kocsube, S., Palagyi, A., Bagi, F., Budakov, D., Stojšin, V., Lazić, S., Bodroža-Solarov, M., Čolović, R., Bekavac, G., Purar, B., Jocković, Đ.,& Mesterhazy, A.. (2018). A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize. in Toxins
Basel : MDPI., 10(9).
https://doi.org/10.3390/toxins10090372

Szabo B, Toth B, Toldine ET, Varga M, Kovacs N, Varga J, Kocsube S, Palagyi A, Bagi F, Budakov D, Stojšin V, Lazić S, Bodroža-Solarov M, Čolović R, Bekavac G, Purar B, Jocković Đ, Mesterhazy A. A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize. in Toxins. 2018;10(9).
doi:10.3390/toxins10090372 .

Szabo, Balazs, Toth, Beata, Toldine, Eva Toth, Varga, Monika, Kovacs, Nandor, Varga, Janos, Kocsube, Sandor, Palagyi, Andrea, Bagi, Ferenc, Budakov, Dragana, Stojšin, Vera, Lazić, Sanja, Bodroža-Solarov, Marija, Čolović, Radmilo, Bekavac, Goran, Purar, Božana, Jocković, Đorđe, Mesterhazy, Akos, "A New Concept to Secure Food Safety Standards against Fusarium Species and Aspergillus Flavus and Their Toxins in Maize" in Toxins, 10, no. 9 (2018),
https://doi.org/10.3390/toxins10090372 . .

TY  - CONF
AU  - Budakov, Dragana
AU  - Nagl, Nevena
AU  - Stojšin, Vera
AU  - Taški-Ajduković, Ksenija
AU  - Bagi, Ferenc
AU  - Neher, Oliver T.
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1465
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Morphological, cultural, pathogenic and genetic characteristics of Macrophomina phaseolina, causer of sugar beet charcoal root rot
EP  - 21
IS  - 11
SP  - 21
VL  - 105
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1465
ER  - 

@conference{
author = "Budakov, Dragana and Nagl, Nevena and Stojšin, Vera and Taški-Ajduković, Ksenija and Bagi, Ferenc and Neher, Oliver T.",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Morphological, cultural, pathogenic and genetic characteristics of Macrophomina phaseolina, causer of sugar beet charcoal root rot",
pages = "21-21",
number = "11",
volume = "105",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1465"
}

Budakov, D., Nagl, N., Stojšin, V., Taški-Ajduković, K., Bagi, F.,& Neher, O. T.. (2015). Morphological, cultural, pathogenic and genetic characteristics of Macrophomina phaseolina, causer of sugar beet charcoal root rot. in Phytopathology
Amer Phytopathological Soc, St Paul., 105(11), 21-21.
https://hdl.handle.net/21.15107/rcub_fiver_1465

Budakov D, Nagl N, Stojšin V, Taški-Ajduković K, Bagi F, Neher OT. Morphological, cultural, pathogenic and genetic characteristics of Macrophomina phaseolina, causer of sugar beet charcoal root rot. in Phytopathology. 2015;105(11):21-21.
https://hdl.handle.net/21.15107/rcub_fiver_1465 .

Budakov, Dragana, Nagl, Nevena, Stojšin, Vera, Taški-Ajduković, Ksenija, Bagi, Ferenc, Neher, Oliver T., "Morphological, cultural, pathogenic and genetic characteristics of Macrophomina phaseolina, causer of sugar beet charcoal root rot" in Phytopathology, 105, no. 11 (2015):21-21,
https://hdl.handle.net/21.15107/rcub_fiver_1465 .

TY  - JOUR
AU  - Malenčić, Đorđe
AU  - Kiprovski, Biljana
AU  - Stojšin, Vera
AU  - Budakov, Dragana
AU  - Pogančev, Gorana
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/2212
AB  - The research was focused on biotic stress caused by Macrophomina phaseolina (Tassi) Goid., a plant pathogen that causes charcoal rot by infecting the root and lower stem of soybean. Differences in pathogenicity of fungi cultures from five isolates (two from sugar beet, one from maize, one from soybean and one from sunflower) have been investigated in seedlings of two soybean cultivars (Meli and Balkan). Comparison of antioxidant systems of soybean seedlings showed different reactions of plants infected with M. phaseolina isolates of different origin. The highest pathogenicity showed isolate from sugar beet ŠR62/4 (2.80), while no significant difference was found for isolates ŠR55(3)/09 (2.58), MphSo (2.32) and MphSu (2.26). The isolate from maize, MphK, showed lowest pathogenicity (1.94). For the most of the biochemical parameters tested (lipid peroxidation, phenylalanine ammonium-lyase activity, total polyphenols, tannins and proanthocyanidins contents) the isolate from sugar beet (ŠR55(3)/09) induced the most significant reaction in soybean seedlings among all isolates investigated. Presence of this pathogen in the field conditions should be further monitored and the crop rotation should be seriously taken into account.
AB  - U radu je ispitivan biotički stres u klijancima soje izazvan gljivom Macrophomina phaseolina (Tassi Goid.), patogenom biljaka i uzročnikom ugljenaste truleži korena i donjeg dela stabla. Različite karakteristike izolata gljive (dobijenih sa šećerne repe (2), kukuruza (1), soje (1) i suncokreta (1)), ispitane su u dve sorte soje (Meli i Balkan). Poređenje antioksidantnih sistema u biljkama soje ukazalo je na različite odgovore između sorti soje inficiranih izolatima M. phaseolina različitog porekla. Izolati su izazvali intenzivnu reakciju u inficiranim klijancima: najveća patogenost zabeležena je kod izolata sa šećerne repe ŠR62/4 (2.80). Izolati ŠR55(3)/09 (2.58), MphSo (2.32) i MphSu (2.26) nisu pokazali statistički značajnu razliku u patogenosti. Izolat sa kukuruza, MphK, pokazao je najnižu patogenost (1.94). Za većinu ispitivanih biohemijskih parametara (lipidna peroksidacija, aktivnost fenilalanin amonijum-liaze, sadržaji ukupnih polifenola, tanina i proantocijanidina), izolat ŠR55(3)/09 sa šećerne repe, izazvao je najintenzivnije i najznačajnije reakcije u klijancima soje u poređenju sa svim ostalim ispitanim izolatima. Njegovo prisustvo u poljskim uslovima se mora podvrgnuti monitoringu, a o rotaciji useva na takvim oranicama se mora povesti više pažnje.
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
T2  - Contemporary Agriculture
T1  - Comparison of oxidative stress parameters in soybean seedlings inoculated with Macrophomina phaseolina from different isolates
T1  - Poređenje parametara oksidativnog stresa u klijancima soje inokulisanim sa različitim izolatima Macrophomina phaseolina
EP  - 472
IS  - 4-5
SP  - 463
VL  - 63
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2212
ER  - 

@article{
author = "Malenčić, Đorđe and Kiprovski, Biljana and Stojšin, Vera and Budakov, Dragana and Pogančev, Gorana",
year = "2014",
abstract = "The research was focused on biotic stress caused by Macrophomina phaseolina (Tassi) Goid., a plant pathogen that causes charcoal rot by infecting the root and lower stem of soybean. Differences in pathogenicity of fungi cultures from five isolates (two from sugar beet, one from maize, one from soybean and one from sunflower) have been investigated in seedlings of two soybean cultivars (Meli and Balkan). Comparison of antioxidant systems of soybean seedlings showed different reactions of plants infected with M. phaseolina isolates of different origin. The highest pathogenicity showed isolate from sugar beet ŠR62/4 (2.80), while no significant difference was found for isolates ŠR55(3)/09 (2.58), MphSo (2.32) and MphSu (2.26). The isolate from maize, MphK, showed lowest pathogenicity (1.94). For the most of the biochemical parameters tested (lipid peroxidation, phenylalanine ammonium-lyase activity, total polyphenols, tannins and proanthocyanidins contents) the isolate from sugar beet (ŠR55(3)/09) induced the most significant reaction in soybean seedlings among all isolates investigated. Presence of this pathogen in the field conditions should be further monitored and the crop rotation should be seriously taken into account., U radu je ispitivan biotički stres u klijancima soje izazvan gljivom Macrophomina phaseolina (Tassi Goid.), patogenom biljaka i uzročnikom ugljenaste truleži korena i donjeg dela stabla. Različite karakteristike izolata gljive (dobijenih sa šećerne repe (2), kukuruza (1), soje (1) i suncokreta (1)), ispitane su u dve sorte soje (Meli i Balkan). Poređenje antioksidantnih sistema u biljkama soje ukazalo je na različite odgovore između sorti soje inficiranih izolatima M. phaseolina različitog porekla. Izolati su izazvali intenzivnu reakciju u inficiranim klijancima: najveća patogenost zabeležena je kod izolata sa šećerne repe ŠR62/4 (2.80). Izolati ŠR55(3)/09 (2.58), MphSo (2.32) i MphSu (2.26) nisu pokazali statistički značajnu razliku u patogenosti. Izolat sa kukuruza, MphK, pokazao je najnižu patogenost (1.94). Za većinu ispitivanih biohemijskih parametara (lipidna peroksidacija, aktivnost fenilalanin amonijum-liaze, sadržaji ukupnih polifenola, tanina i proantocijanidina), izolat ŠR55(3)/09 sa šećerne repe, izazvao je najintenzivnije i najznačajnije reakcije u klijancima soje u poređenju sa svim ostalim ispitanim izolatima. Njegovo prisustvo u poljskim uslovima se mora podvrgnuti monitoringu, a o rotaciji useva na takvim oranicama se mora povesti više pažnje.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture",
journal = "Contemporary Agriculture",
title = "Comparison of oxidative stress parameters in soybean seedlings inoculated with Macrophomina phaseolina from different isolates, Poređenje parametara oksidativnog stresa u klijancima soje inokulisanim sa različitim izolatima Macrophomina phaseolina",
pages = "472-463",
number = "4-5",
volume = "63",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2212"
}

Malenčić, Đ., Kiprovski, B., Stojšin, V., Budakov, D.,& Pogančev, G.. (2014). Comparison of oxidative stress parameters in soybean seedlings inoculated with Macrophomina phaseolina from different isolates. in Contemporary Agriculture
Novi Sad : University of Novi Sad, Faculty of Agriculture., 63(4-5), 463-472.
https://hdl.handle.net/21.15107/rcub_fiver_2212

Malenčić Đ, Kiprovski B, Stojšin V, Budakov D, Pogančev G. Comparison of oxidative stress parameters in soybean seedlings inoculated with Macrophomina phaseolina from different isolates. in Contemporary Agriculture. 2014;63(4-5):463-472.
https://hdl.handle.net/21.15107/rcub_fiver_2212 .

Malenčić, Đorđe, Kiprovski, Biljana, Stojšin, Vera, Budakov, Dragana, Pogančev, Gorana, "Comparison of oxidative stress parameters in soybean seedlings inoculated with Macrophomina phaseolina from different isolates" in Contemporary Agriculture, 63, no. 4-5 (2014):463-472,
https://hdl.handle.net/21.15107/rcub_fiver_2212 .

TY  - JOUR
AU  - Kiprovski, Biljana
AU  - Malenčić, Ðorđe
AU  - Popović, Milan
AU  - Stojšin, Vera
AU  - Budakov, Dragana
AU  - Ćurčić, Živko
AU  - Danojević, Dario
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1360
AB  - The correlation between intensity of lipid peroxidation and changes in antioxidant capacity of sugar beet plants (cv. `Drena') infected with Rhizoctonia solani Kuhn isolate ( AG 2- 2 IIIB group) was studied. Successful inoculation was confirmed by the presence of infection cushions in a cross section of leaf petioles. On the 7th day of the experiment, phenylalanine ammonia-lyase ( PAL; EC. 4.3.1.5) activity was in negative correlation with intensified lipid peroxidation process in leaves of sugar beet plants ( r= - 0.99). Also, in leaves and roots of inoculated sugar beet plants, total flavonoids content ( 35% and 20%, respectively) and 1,1- diphenyl- 2-picrylhydrazyl (DPPH)-scavenging activity ( 80% and 55%, respectively) were significantly reduced. Necrotic processes resulting from R. solani infection of sugar beet plants was followed by induction of plant phenolics metabolism; however, antioxidant capacity of these plants was reduced.
PB  - Springer, Dordrecht
T2  - Phytoparasitica
T1  - Correlation between lipid peroxidation and phenolics content in leaves and roots of sugar beet infected with Rhizoctonia solani
EP  - 203
IS  - 2
SP  - 199
VL  - 42
DO  - 10.1007/s12600-013-0352-7
ER  - 

@article{
author = "Kiprovski, Biljana and Malenčić, Ðorđe and Popović, Milan and Stojšin, Vera and Budakov, Dragana and Ćurčić, Živko and Danojević, Dario",
year = "2014",
abstract = "The correlation between intensity of lipid peroxidation and changes in antioxidant capacity of sugar beet plants (cv. `Drena') infected with Rhizoctonia solani Kuhn isolate ( AG 2- 2 IIIB group) was studied. Successful inoculation was confirmed by the presence of infection cushions in a cross section of leaf petioles. On the 7th day of the experiment, phenylalanine ammonia-lyase ( PAL; EC. 4.3.1.5) activity was in negative correlation with intensified lipid peroxidation process in leaves of sugar beet plants ( r= - 0.99). Also, in leaves and roots of inoculated sugar beet plants, total flavonoids content ( 35% and 20%, respectively) and 1,1- diphenyl- 2-picrylhydrazyl (DPPH)-scavenging activity ( 80% and 55%, respectively) were significantly reduced. Necrotic processes resulting from R. solani infection of sugar beet plants was followed by induction of plant phenolics metabolism; however, antioxidant capacity of these plants was reduced.",
publisher = "Springer, Dordrecht",
journal = "Phytoparasitica",
title = "Correlation between lipid peroxidation and phenolics content in leaves and roots of sugar beet infected with Rhizoctonia solani",
pages = "203-199",
number = "2",
volume = "42",
doi = "10.1007/s12600-013-0352-7"
}

Kiprovski, B., Malenčić, Ð., Popović, M., Stojšin, V., Budakov, D., Ćurčić, Ž.,& Danojević, D.. (2014). Correlation between lipid peroxidation and phenolics content in leaves and roots of sugar beet infected with Rhizoctonia solani. in Phytoparasitica
Springer, Dordrecht., 42(2), 199-203.
https://doi.org/10.1007/s12600-013-0352-7

Kiprovski B, Malenčić Ð, Popović M, Stojšin V, Budakov D, Ćurčić Ž, Danojević D. Correlation between lipid peroxidation and phenolics content in leaves and roots of sugar beet infected with Rhizoctonia solani. in Phytoparasitica. 2014;42(2):199-203.
doi:10.1007/s12600-013-0352-7 .

Kiprovski, Biljana, Malenčić, Ðorđe, Popović, Milan, Stojšin, Vera, Budakov, Dragana, Ćurčić, Živko, Danojević, Dario, "Correlation between lipid peroxidation and phenolics content in leaves and roots of sugar beet infected with Rhizoctonia solani" in Phytoparasitica, 42, no. 2 (2014):199-203,
https://doi.org/10.1007/s12600-013-0352-7 . .

TY  - JOUR
AU  - Budakov, Dragana
AU  - Nagl, Nevena
AU  - Stojšin, Vera
AU  - Bagi, Ferenc
AU  - Danojević, Dario
AU  - Neher, Oliver T.
AU  - Taški-Ajduković, Ksenija
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1384
AB  - Cercospora beticola, causal agent of Cercospora leaf spot (CLS) of sugar beet, is primarily controlled by fungicides. Benzimidazole and demethylation inhibiting fungicides, including carbendazim and flutriafol, have been widely used in Serbia. Since these fungicide groups have a site-specific mode of action, there is a high risk for developing resistance in target organisms, which is the most important limiting factor in Cercospora leaf spot chemical control. A rapid identification of flutriafol and carbendazim resistance can help researchers in examining the potential of different fungicide resistance management practices, as well as in selection of fungicides for use in the areas where resistance has occurred. One hundred single-conidia isolates were collected from 70 representative locations of the sugar beet production region in Serbia. Evaluation of the isolates' sensitivity was based on the reduction of mycelial growth on medium amended with 1.25 mu g mL(-1) flutriafol and 5 mu g mL(-1) carbendazim. Resistance to flutriafol and carbendazim was detected in 16% and 96% of the tested isolates, respectively. All isolates resistant to flutriafol were resistant to carbendazim as well, which is the first report of a double resistance to fungicides in C. beticola. Detection of the isolates resistant to flutriafol and carbendazim using Cleaved Amplified Polymorphic Sequence (CAPS) markers confirmed the results of the in vitro tests. The efficacy of carbendazim, flutriafol, azoxystrobin, and tetraconazole at commercially recommended doses was evaluated in field trials where sugar beet plants in plots were inoculated with a mixture of isolates either sensitive and/or resistant to flutriafol and carbendazim. Carbendazim and flutriafol efficacy was very low in plots inoculated with isolates resistant to these fungicides. Presented results will contribute to development of a pathogen population sensitivity monitoring strategy that could be used for an effective CLS management in the region.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol
EP  - 126
SP  - 120
VL  - 66
DO  - 10.1016/j.cropro.2014.09.010
ER  - 

@article{
author = "Budakov, Dragana and Nagl, Nevena and Stojšin, Vera and Bagi, Ferenc and Danojević, Dario and Neher, Oliver T. and Taški-Ajduković, Ksenija",
year = "2014",
abstract = "Cercospora beticola, causal agent of Cercospora leaf spot (CLS) of sugar beet, is primarily controlled by fungicides. Benzimidazole and demethylation inhibiting fungicides, including carbendazim and flutriafol, have been widely used in Serbia. Since these fungicide groups have a site-specific mode of action, there is a high risk for developing resistance in target organisms, which is the most important limiting factor in Cercospora leaf spot chemical control. A rapid identification of flutriafol and carbendazim resistance can help researchers in examining the potential of different fungicide resistance management practices, as well as in selection of fungicides for use in the areas where resistance has occurred. One hundred single-conidia isolates were collected from 70 representative locations of the sugar beet production region in Serbia. Evaluation of the isolates' sensitivity was based on the reduction of mycelial growth on medium amended with 1.25 mu g mL(-1) flutriafol and 5 mu g mL(-1) carbendazim. Resistance to flutriafol and carbendazim was detected in 16% and 96% of the tested isolates, respectively. All isolates resistant to flutriafol were resistant to carbendazim as well, which is the first report of a double resistance to fungicides in C. beticola. Detection of the isolates resistant to flutriafol and carbendazim using Cleaved Amplified Polymorphic Sequence (CAPS) markers confirmed the results of the in vitro tests. The efficacy of carbendazim, flutriafol, azoxystrobin, and tetraconazole at commercially recommended doses was evaluated in field trials where sugar beet plants in plots were inoculated with a mixture of isolates either sensitive and/or resistant to flutriafol and carbendazim. Carbendazim and flutriafol efficacy was very low in plots inoculated with isolates resistant to these fungicides. Presented results will contribute to development of a pathogen population sensitivity monitoring strategy that could be used for an effective CLS management in the region.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol",
pages = "126-120",
volume = "66",
doi = "10.1016/j.cropro.2014.09.010"
}

Budakov, D., Nagl, N., Stojšin, V., Bagi, F., Danojević, D., Neher, O. T.,& Taški-Ajduković, K.. (2014). Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol. in Crop Protection
Elsevier Sci Ltd, Oxford., 66, 120-126.
https://doi.org/10.1016/j.cropro.2014.09.010

Budakov D, Nagl N, Stojšin V, Bagi F, Danojević D, Neher OT, Taški-Ajduković K. Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol. in Crop Protection. 2014;66:120-126.
doi:10.1016/j.cropro.2014.09.010 .

Budakov, Dragana, Nagl, Nevena, Stojšin, Vera, Bagi, Ferenc, Danojević, Dario, Neher, Oliver T., Taški-Ajduković, Ksenija, "Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol" in Crop Protection, 66 (2014):120-126,
https://doi.org/10.1016/j.cropro.2014.09.010 . .

TY  - CONF
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Nagl, Nevena
AU  - Bagi, Ferenc
AU  - Danojević, Dario
AU  - Taški-Ajduković, Ksenija
AU  - Neher, Oliver T.
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1219
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Sensitivity of Cercospora beticola from Serbia to benzimidazole and sterol demethylation inhibiting fungicides
EP  - 21
IS  - 6
SP  - 21
VL  - 103
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1219
ER  - 

@conference{
author = "Budakov, Dragana and Stojšin, Vera and Nagl, Nevena and Bagi, Ferenc and Danojević, Dario and Taški-Ajduković, Ksenija and Neher, Oliver T.",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Sensitivity of Cercospora beticola from Serbia to benzimidazole and sterol demethylation inhibiting fungicides",
pages = "21-21",
number = "6",
volume = "103",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1219"
}

Budakov, D., Stojšin, V., Nagl, N., Bagi, F., Danojević, D., Taški-Ajduković, K.,& Neher, O. T.. (2013). Sensitivity of Cercospora beticola from Serbia to benzimidazole and sterol demethylation inhibiting fungicides. in Phytopathology
Amer Phytopathological Soc, St Paul., 103(6), 21-21.
https://hdl.handle.net/21.15107/rcub_fiver_1219

Budakov D, Stojšin V, Nagl N, Bagi F, Danojević D, Taški-Ajduković K, Neher OT. Sensitivity of Cercospora beticola from Serbia to benzimidazole and sterol demethylation inhibiting fungicides. in Phytopathology. 2013;103(6):21-21.
https://hdl.handle.net/21.15107/rcub_fiver_1219 .

Budakov, Dragana, Stojšin, Vera, Nagl, Nevena, Bagi, Ferenc, Danojević, Dario, Taški-Ajduković, Ksenija, Neher, Oliver T., "Sensitivity of Cercospora beticola from Serbia to benzimidazole and sterol demethylation inhibiting fungicides" in Phytopathology, 103, no. 6 (2013):21-21,
https://hdl.handle.net/21.15107/rcub_fiver_1219 .

TY  - JOUR
AU  - Kiprovski, Biljana
AU  - Malenčić, Ðorđe
AU  - Popović, M.
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Balešević-Tubić, Svetlana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1078
AB  - Changes in enzymatic and non-enzymatic antioxidant systems in soybean (cv. Becejka) and maize (hybrid NS 640) seedlings were studied after inoculation with Rhizoctonia solani AG 2-2 IIIB. Enzymes such as plant peroxidases and phenylalanine ammonia-lyase were induced in the analyzed organs of both plant species. Nevertheless, increase in superoxide dismutase activity was recorded only in infected maize roots. After inoculation, lipid peroxidation was enhanced only in soybean plants. Seedlings showed ability to accumulate phenolic compounds, some of which correlated with disease intensity. A significant decrease in total carotenoid amounts was recorded in infected soybean and maize plants. After R. solani infection, the enzymatic and nonenzymatic antioxidant systems were more efficient in maize than in soybean seedlings.
PB  - Edizioni Ets, Pisa
T2  - Journal of Plant Pathology
T1  - Antioxidant systems in soybean and maize seedlings infected with rhizoctonia solani
EP  - 324
IS  - 2
SP  - 313
VL  - 94
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1078
ER  - 

@article{
author = "Kiprovski, Biljana and Malenčić, Ðorđe and Popović, M. and Budakov, Dragana and Stojšin, Vera and Balešević-Tubić, Svetlana",
year = "2012",
abstract = "Changes in enzymatic and non-enzymatic antioxidant systems in soybean (cv. Becejka) and maize (hybrid NS 640) seedlings were studied after inoculation with Rhizoctonia solani AG 2-2 IIIB. Enzymes such as plant peroxidases and phenylalanine ammonia-lyase were induced in the analyzed organs of both plant species. Nevertheless, increase in superoxide dismutase activity was recorded only in infected maize roots. After inoculation, lipid peroxidation was enhanced only in soybean plants. Seedlings showed ability to accumulate phenolic compounds, some of which correlated with disease intensity. A significant decrease in total carotenoid amounts was recorded in infected soybean and maize plants. After R. solani infection, the enzymatic and nonenzymatic antioxidant systems were more efficient in maize than in soybean seedlings.",
publisher = "Edizioni Ets, Pisa",
journal = "Journal of Plant Pathology",
title = "Antioxidant systems in soybean and maize seedlings infected with rhizoctonia solani",
pages = "324-313",
number = "2",
volume = "94",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1078"
}

Kiprovski, B., Malenčić, Ð., Popović, M., Budakov, D., Stojšin, V.,& Balešević-Tubić, S.. (2012). Antioxidant systems in soybean and maize seedlings infected with rhizoctonia solani. in Journal of Plant Pathology
Edizioni Ets, Pisa., 94(2), 313-324.
https://hdl.handle.net/21.15107/rcub_fiver_1078

Kiprovski B, Malenčić Ð, Popović M, Budakov D, Stojšin V, Balešević-Tubić S. Antioxidant systems in soybean and maize seedlings infected with rhizoctonia solani. in Journal of Plant Pathology. 2012;94(2):313-324.
https://hdl.handle.net/21.15107/rcub_fiver_1078 .

Kiprovski, Biljana, Malenčić, Ðorđe, Popović, M., Budakov, Dragana, Stojšin, Vera, Balešević-Tubić, Svetlana, "Antioxidant systems in soybean and maize seedlings infected with rhizoctonia solani" in Journal of Plant Pathology, 94, no. 2 (2012):313-324,
https://hdl.handle.net/21.15107/rcub_fiver_1078 .

TY  - JOUR
AU  - Vučković, Jovana
AU  - Bagi, Ferenc
AU  - Bodroža-Solarov, Marija
AU  - Stojšin, Vera
AU  - Budakov, Dragana
AU  - Ugrenović, Vladan
AU  - Aćimović, Milica
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/2634
AB  - Gajenje alternativnih biljnih vrsta, kao što je spelta (Triticum aestivum ssp. spelta), doprinosi održavanju agroekoloških sistema gajenja, što naročito ima značaja u organskoj proizvodnji. Novija istraživanja su pokazala da na zrnima pšenice dominiraju mikroorganizmi iz roda Alternaria. Značaj Alternaria spp. se ogleda ne samo u narušavanju kvaliteta zrna pšenice, već i u tome što mnoge vrste ovog roda produkuju toksične metabolite koji su štetni po zdravlje ljudi i životinja. Cilj ovih istraživanja je bio da se oceni intenzitet zaraze roda Alternaria na pet genotipova spelte, kao i da se uporedi intenzitet zaraze na oljuštenim i zrnima sa plevičastim omotačima, uz determinaciju dominantnih vrsta Alternaria spp. Utvrđeno je da je genotip 1 bio najmanje zaražen i da su oljuštena zrna imala manji stepen zaraze za oko 50% u odnosu na zrna sa plevičastim omotačima. Plevičasti omotači su se pokazali kao fizička zaštita od patogena. Determinisane su vrste A. tenuissima i A. alternata, od kojih je dominirala A. tenuissima (90%).
AB  - Growing alternative cereals, such as Triticum aestivum ssp. spelta, encourages agro-ecological system of cultivation, which is of great importance in organic production. Recent studies have shown that fungi of the genus Alternaria dominates on wheat kernels. Besides pathogenicity and reduction of quality of kernels, several Alternaria species are known producers of toxic secondary metabolites - Alternaria mycotoxins, which might be harmful for human and animal health. The aim of this work was to evaluate the intensity of Alternaria spp. infection on spelt kernels, hulled and dehulled and to determine Alternaria species. It was found that dehulled kernels were up to 50% less infected compared to hulled kernels. The results indicate that the glumes protect the kernel inside from fungal penetration. Determinated species were A. tenuissima and A. alternata, with predominance of A. tenuissima (90%)
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
T2  - Biljni lekar
T1  - Intenzitet zaraze zrna spelte gljivama roda Alternaria
T1  - The intensity of Alternaria spp. infection on spelt kernels
EP  - 55
IS  - 1
SP  - 50
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2634
ER  - 

@article{
author = "Vučković, Jovana and Bagi, Ferenc and Bodroža-Solarov, Marija and Stojšin, Vera and Budakov, Dragana and Ugrenović, Vladan and Aćimović, Milica",
year = "2012",
abstract = "Gajenje alternativnih biljnih vrsta, kao što je spelta (Triticum aestivum ssp. spelta), doprinosi održavanju agroekoloških sistema gajenja, što naročito ima značaja u organskoj proizvodnji. Novija istraživanja su pokazala da na zrnima pšenice dominiraju mikroorganizmi iz roda Alternaria. Značaj Alternaria spp. se ogleda ne samo u narušavanju kvaliteta zrna pšenice, već i u tome što mnoge vrste ovog roda produkuju toksične metabolite koji su štetni po zdravlje ljudi i životinja. Cilj ovih istraživanja je bio da se oceni intenzitet zaraze roda Alternaria na pet genotipova spelte, kao i da se uporedi intenzitet zaraze na oljuštenim i zrnima sa plevičastim omotačima, uz determinaciju dominantnih vrsta Alternaria spp. Utvrđeno je da je genotip 1 bio najmanje zaražen i da su oljuštena zrna imala manji stepen zaraze za oko 50% u odnosu na zrna sa plevičastim omotačima. Plevičasti omotači su se pokazali kao fizička zaštita od patogena. Determinisane su vrste A. tenuissima i A. alternata, od kojih je dominirala A. tenuissima (90%)., Growing alternative cereals, such as Triticum aestivum ssp. spelta, encourages agro-ecological system of cultivation, which is of great importance in organic production. Recent studies have shown that fungi of the genus Alternaria dominates on wheat kernels. Besides pathogenicity and reduction of quality of kernels, several Alternaria species are known producers of toxic secondary metabolites - Alternaria mycotoxins, which might be harmful for human and animal health. The aim of this work was to evaluate the intensity of Alternaria spp. infection on spelt kernels, hulled and dehulled and to determine Alternaria species. It was found that dehulled kernels were up to 50% less infected compared to hulled kernels. The results indicate that the glumes protect the kernel inside from fungal penetration. Determinated species were A. tenuissima and A. alternata, with predominance of A. tenuissima (90%)",
publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture",
journal = "Biljni lekar",
title = "Intenzitet zaraze zrna spelte gljivama roda Alternaria, The intensity of Alternaria spp. infection on spelt kernels",
pages = "55-50",
number = "1",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2634"
}

Vučković, J., Bagi, F., Bodroža-Solarov, M., Stojšin, V., Budakov, D., Ugrenović, V.,& Aćimović, M.. (2012). Intenzitet zaraze zrna spelte gljivama roda Alternaria. in Biljni lekar
Novi Sad : University of Novi Sad, Faculty of Agriculture., 40(1), 50-55.
https://hdl.handle.net/21.15107/rcub_fiver_2634

Vučković J, Bagi F, Bodroža-Solarov M, Stojšin V, Budakov D, Ugrenović V, Aćimović M. Intenzitet zaraze zrna spelte gljivama roda Alternaria. in Biljni lekar. 2012;40(1):50-55.
https://hdl.handle.net/21.15107/rcub_fiver_2634 .

Vučković, Jovana, Bagi, Ferenc, Bodroža-Solarov, Marija, Stojšin, Vera, Budakov, Dragana, Ugrenović, Vladan, Aćimović, Milica, "Intenzitet zaraze zrna spelte gljivama roda Alternaria" in Biljni lekar, 40, no. 1 (2012):50-55,
https://hdl.handle.net/21.15107/rcub_fiver_2634 .

TY  - JOUR
AU  - Budakov, Dragana
AU  - Nagl, Nevena
AU  - Taški-Ajduković, Ksenija
AU  - Stojšin, Vera
AU  - Bagi, Ferenc
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1058
AB  - The most fungal DNA isolation protocols are designed to obtain high amounts of very pure DNA, requiring large fungal cultures and extraction procedures with many purification steps. Since the PCR does not require high purity DNA, the aim of this investigation was to evaluate three fast and simple fungal DNA isolation protocols for further use in Cercospora PCR based research. The purity and quantity of isolated DNAs were determined spectrophotometrically, electrophoretically and by PCR reaction with universal primers. The amounts of DNA evaluated on agarose gels, isolated by protocols A and C, did not correspond to the spectrophotometrical values, probably due to RNA impurities. In samples isolated by protocol B these impurities were not detected and the DNA concentrations were more similar. Neither protocol eliminated impurities such as carbohydrates and phenol. The average DNA yield of protocol A was 1.04 μg/μl, protocol B 0.88 μg/μl, and protocol C 0.55 μg/μl. The DNA quality most suitable for PCR analysis was obtained by protocol A, where amplification product with universal primers was detected in all DNA samples. The amplification product was detected in 87% of samples isolated by protocol C and in only 60% of samples isolated by protocol B. Although DNA obtained by protocol A had the highest yield and best quality, the isolation protocol C should be also recommended, for it does not require phenol, chlorophorm or liquid nitrogen.
AB  - Sa ciljem uspostavljanja odgovarajućeg protokola izolacije DNK zaprimenu u istraživanjima zasnovanim na PCR analizi, u radu su ispitana tri jednostavna i brza metoda za DNK izolaciju iz Cercospora beticola Sacc. Čistoća i količina izolovane DNK je određena spektrofotometrijski, elektroforetski i PCR reakcijom sa univerzalnim prajmerima. Količina DNK procenjena na agaroznom gelu, koja je izolovana pomoću protokola A i C nije odgovarala spektrofotometrijskim vrednostima, verovatno usled prisustva RNK nečistoća. U uzorcima izolovanim pomoću protokola B ove nečistoće nisu detektovane, pa su DNK koncentracije bile podudarne. Ni u jednom protokolu nisu eliminisane nečistoće, kao što su ugljeni hidrati i fenoli. PCR amplifikacija je uočena kod svih DNK uzoraka izolovanih protokolom A, kod 60% uzoraka izolovanih protokolom B i kod 87% uzoraka izolovanih protokolom C. Iako je protokol za izolaciju A dao najbolje rezultate, protokol C se takođe može preporučiti, jer ne zahteva upotrebu fenola, hloroforma i tečnog azota.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Evaluation of rapid protocols for DNA isolation from Cercospora beticola Sacc.
T1  - Procena protokola za brzu izolaciju DNK iz Cercospora beticola Sacc.
EP  - 207
IS  - 2
SP  - 202
VL  - 49
DO  - 10.5937/ratpov49-1198
ER  - 

@article{
author = "Budakov, Dragana and Nagl, Nevena and Taški-Ajduković, Ksenija and Stojšin, Vera and Bagi, Ferenc",
year = "2012",
abstract = "The most fungal DNA isolation protocols are designed to obtain high amounts of very pure DNA, requiring large fungal cultures and extraction procedures with many purification steps. Since the PCR does not require high purity DNA, the aim of this investigation was to evaluate three fast and simple fungal DNA isolation protocols for further use in Cercospora PCR based research. The purity and quantity of isolated DNAs were determined spectrophotometrically, electrophoretically and by PCR reaction with universal primers. The amounts of DNA evaluated on agarose gels, isolated by protocols A and C, did not correspond to the spectrophotometrical values, probably due to RNA impurities. In samples isolated by protocol B these impurities were not detected and the DNA concentrations were more similar. Neither protocol eliminated impurities such as carbohydrates and phenol. The average DNA yield of protocol A was 1.04 μg/μl, protocol B 0.88 μg/μl, and protocol C 0.55 μg/μl. The DNA quality most suitable for PCR analysis was obtained by protocol A, where amplification product with universal primers was detected in all DNA samples. The amplification product was detected in 87% of samples isolated by protocol C and in only 60% of samples isolated by protocol B. Although DNA obtained by protocol A had the highest yield and best quality, the isolation protocol C should be also recommended, for it does not require phenol, chlorophorm or liquid nitrogen., Sa ciljem uspostavljanja odgovarajućeg protokola izolacije DNK zaprimenu u istraživanjima zasnovanim na PCR analizi, u radu su ispitana tri jednostavna i brza metoda za DNK izolaciju iz Cercospora beticola Sacc. Čistoća i količina izolovane DNK je određena spektrofotometrijski, elektroforetski i PCR reakcijom sa univerzalnim prajmerima. Količina DNK procenjena na agaroznom gelu, koja je izolovana pomoću protokola A i C nije odgovarala spektrofotometrijskim vrednostima, verovatno usled prisustva RNK nečistoća. U uzorcima izolovanim pomoću protokola B ove nečistoće nisu detektovane, pa su DNK koncentracije bile podudarne. Ni u jednom protokolu nisu eliminisane nečistoće, kao što su ugljeni hidrati i fenoli. PCR amplifikacija je uočena kod svih DNK uzoraka izolovanih protokolom A, kod 60% uzoraka izolovanih protokolom B i kod 87% uzoraka izolovanih protokolom C. Iako je protokol za izolaciju A dao najbolje rezultate, protokol C se takođe može preporučiti, jer ne zahteva upotrebu fenola, hloroforma i tečnog azota.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Evaluation of rapid protocols for DNA isolation from Cercospora beticola Sacc., Procena protokola za brzu izolaciju DNK iz Cercospora beticola Sacc.",
pages = "207-202",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1198"
}

Budakov, D., Nagl, N., Taški-Ajduković, K., Stojšin, V.,& Bagi, F.. (2012). Evaluation of rapid protocols for DNA isolation from Cercospora beticola Sacc.. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 202-207.
https://doi.org/10.5937/ratpov49-1198

Budakov D, Nagl N, Taški-Ajduković K, Stojšin V, Bagi F. Evaluation of rapid protocols for DNA isolation from Cercospora beticola Sacc.. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2012;49(2):202-207.
doi:10.5937/ratpov49-1198 .

Budakov, Dragana, Nagl, Nevena, Taški-Ajduković, Ksenija, Stojšin, Vera, Bagi, Ferenc, "Evaluation of rapid protocols for DNA isolation from Cercospora beticola Sacc." in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 49, no. 2 (2012):202-207,
https://doi.org/10.5937/ratpov49-1198 . .

TY  - JOUR
AU  - Lazić, Sanja
AU  - Šunjka, Dragana B.
AU  - Grahovac, Nada
AU  - Guzsvány, Valéria
AU  - Bagi, Ferenc
AU  - Budakov, Dragana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1131
AB  - A rapid and simple method for simultaneous determination of acetamiprid and its metabolite 6-chloronicotinic acid in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C18 column with gradient elution. Analytes' determination and quantification were performed by high performance liquid chromatography (HPLC) with diode-array detector and chromatograms were extracted at 230 nm. Extraction efficiency experiments demonstrated the ability of this method to extract neonicotinoids from sweet cherry samples. These insecticides were extracted with a mixture of acetonitril/0.1N ammonium-chloride (8/2, v/v). The average recoveries of acetamiprid and 6-chlornicotinic acid from sweet cherry samples were in the range of 95-101% and 73-83%, respectively, with the associated relative standard deviations (RSDs)  lt 5%. Expanded measurement uncertainties for the analyzed compounds were 2.7 and 3.01%. The limit of quantification (LOQ) was 10 μg/kg and 30 μg/kg for acetamiprid and 6-chloronicotinic acid, respectively. Thus, the developed HPLC/DAD method can be considered a useful tool for sensitive and rapid determination of acetamiprid and 6-chloronicotinic acid. Hence, the method may find further application in the analysis of real sweet cherry samples contaminated with these insecticides at a ppb level.
AB  - U radu je predstavljena jednostavna metoda za određivanje acetamiprida i njegovog metabolita, 6-hlornikotinske kiseline, u uzorcima trešanja. Metoda je bazirana na primeni reverzno-faznog razdvajanja na C18 koloni primenom gradijentnog eluiranja. Određivanje i kvantifikacija analita je vršena tečnom hromatografijom (HPLC) sa DAD detektorom, pri čemu je korišćena talasna dužina od 230 nm. Tačnost metode je ocenjena procenom merne nesigurnosti. Ekstrakcija acetamiprida i 6-hlornikotinske kiseline iz uzoraka trešanja je vršena smešom acetonitril/amonijum-hlorid (0,1N) u odnosu 80:20 (v/v). Sva merenja su vršena u tri ponavljanja, pri čemu su dobijeni prinosi određivanja acetamiprida i 6-hlornikotinske kiseline u rasponima 95-101% i 73-83%, respektivno. Relativne standardne devijacije (RSD) merenja su u svim slučajevima bile ispod 5%. Limiti kvantifikacije za acetamiprid i 6-HNK iznosili su 10 i 30 μg/kg, respektivno. Kombinovana merna nesigurnost rezultata analize acetamiprida i njegovog metabolita procenjena je na 1,35, odnosno 1,50%, a proširena na 2,7 i 3,01%, upotrebom faktora pokrivanja (k=2) koji odgovara nivou poverenja od 95%, za normalnu raspodelu. Nakon validacije i procene merne neizvesnosti dobijeni rezultati pokazuju da se razvijena HPLC/DAD metoda može primeniti za određivanje sadržaja acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja i relevantnim matriksima kontaminiranim ovim jedinjenjima.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples
T1  - Primena tečne hromatografije sa DAD detektorom za određivanje ostataka acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja
EP  - 329
IS  - 4
SP  - 321
VL  - 27
DO  - 10.2298/PIF1204321L
ER  - 

@article{
author = "Lazić, Sanja and Šunjka, Dragana B. and Grahovac, Nada and Guzsvány, Valéria and Bagi, Ferenc and Budakov, Dragana",
year = "2012",
abstract = "A rapid and simple method for simultaneous determination of acetamiprid and its metabolite 6-chloronicotinic acid in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C18 column with gradient elution. Analytes' determination and quantification were performed by high performance liquid chromatography (HPLC) with diode-array detector and chromatograms were extracted at 230 nm. Extraction efficiency experiments demonstrated the ability of this method to extract neonicotinoids from sweet cherry samples. These insecticides were extracted with a mixture of acetonitril/0.1N ammonium-chloride (8/2, v/v). The average recoveries of acetamiprid and 6-chlornicotinic acid from sweet cherry samples were in the range of 95-101% and 73-83%, respectively, with the associated relative standard deviations (RSDs)  lt 5%. Expanded measurement uncertainties for the analyzed compounds were 2.7 and 3.01%. The limit of quantification (LOQ) was 10 μg/kg and 30 μg/kg for acetamiprid and 6-chloronicotinic acid, respectively. Thus, the developed HPLC/DAD method can be considered a useful tool for sensitive and rapid determination of acetamiprid and 6-chloronicotinic acid. Hence, the method may find further application in the analysis of real sweet cherry samples contaminated with these insecticides at a ppb level., U radu je predstavljena jednostavna metoda za određivanje acetamiprida i njegovog metabolita, 6-hlornikotinske kiseline, u uzorcima trešanja. Metoda je bazirana na primeni reverzno-faznog razdvajanja na C18 koloni primenom gradijentnog eluiranja. Određivanje i kvantifikacija analita je vršena tečnom hromatografijom (HPLC) sa DAD detektorom, pri čemu je korišćena talasna dužina od 230 nm. Tačnost metode je ocenjena procenom merne nesigurnosti. Ekstrakcija acetamiprida i 6-hlornikotinske kiseline iz uzoraka trešanja je vršena smešom acetonitril/amonijum-hlorid (0,1N) u odnosu 80:20 (v/v). Sva merenja su vršena u tri ponavljanja, pri čemu su dobijeni prinosi određivanja acetamiprida i 6-hlornikotinske kiseline u rasponima 95-101% i 73-83%, respektivno. Relativne standardne devijacije (RSD) merenja su u svim slučajevima bile ispod 5%. Limiti kvantifikacije za acetamiprid i 6-HNK iznosili su 10 i 30 μg/kg, respektivno. Kombinovana merna nesigurnost rezultata analize acetamiprida i njegovog metabolita procenjena je na 1,35, odnosno 1,50%, a proširena na 2,7 i 3,01%, upotrebom faktora pokrivanja (k=2) koji odgovara nivou poverenja od 95%, za normalnu raspodelu. Nakon validacije i procene merne neizvesnosti dobijeni rezultati pokazuju da se razvijena HPLC/DAD metoda može primeniti za određivanje sadržaja acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja i relevantnim matriksima kontaminiranim ovim jedinjenjima.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples, Primena tečne hromatografije sa DAD detektorom za određivanje ostataka acetamiprida i 6-hlornikotinske kiseline u uzorcima trešanja",
pages = "329-321",
number = "4",
volume = "27",
doi = "10.2298/PIF1204321L"
}

Lazić, S., Šunjka, D. B., Grahovac, N., Guzsvány, V., Bagi, F.,& Budakov, D.. (2012). Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 27(4), 321-329.
https://doi.org/10.2298/PIF1204321L

Lazić S, Šunjka DB, Grahovac N, Guzsvány V, Bagi F, Budakov D. Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples. in Pesticidi i fitomedicina. 2012;27(4):321-329.
doi:10.2298/PIF1204321L .

Lazić, Sanja, Šunjka, Dragana B., Grahovac, Nada, Guzsvány, Valéria, Bagi, Ferenc, Budakov, Dragana, "Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples" in Pesticidi i fitomedicina, 27, no. 4 (2012):321-329,
https://doi.org/10.2298/PIF1204321L . .

TY  - JOUR
AU  - Bagi, Ferenc
AU  - Gvozdanović-Varga, Jelica
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Janićijević, Milana
AU  - Šantić, Marinela
AU  - Jasnić, Stevan
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/940
AB  - During 2009, a total of 27 fall garlic cultivars were evaluated for Onion yellow dwarf virus - OYDV and Leek yellow stripe virus - LYSV disease intensity. The presence of virus was confirmed with DAS-ELISA test. Out of 270 tested samples, 60.4% proved to be infected with at least one of viral species. OYDV was found in 60.0%, and LYSV in 3.0% of analyzed plants. In 2.6% of samples mixed contamination with both viruses was determined. Percent of infection of tested garlic cultivars varied from 0 to 100% with OYDV and 0-30% with LYSV.
AB  - Tokom 2009. godine utvrđen je intenzitet zaraze 27 genotipova jesenjeg belog luka virusima žute patuljavosti crnog luka (Onion yellow dwarf virus - OYDV) i žute prugavosti praziluka (Leek yellow stripe virus - LYSV). Prisustvo virusa dokazano je pomoću DAS -ELISA testa. Od 270 testiranih uzoraka u 60,4% je utvrđena zaraza sa bar jednim od dva ispitivana virusa. OYDV je bio utvrđen u 60,0%, a LYSV u 3,0% analiziranih biljaka. U 2,6 % uzoraka utvrđena je mešovita zaraza sa oba virusa. Zaraženost ispitivanih genotipova jesenjeg belog luka sa OYDV se kretala u granicama 0-100%, a sa LYSV 0-30%.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Incidence of onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYMV) on garlic
T1  - Intenzitet zaraze belog luka virusom žute patuljavosti crnog luka (OYDV) i virusom žute prugavosti praziluka (LYMV)
EP  - 393
IS  - 4
SP  - 389
VL  - 39
UR  - https://hdl.handle.net/21.15107/rcub_fiver_940
ER  - 

@article{
author = "Bagi, Ferenc and Gvozdanović-Varga, Jelica and Budakov, Dragana and Stojšin, Vera and Janićijević, Milana and Šantić, Marinela and Jasnić, Stevan",
year = "2011",
abstract = "During 2009, a total of 27 fall garlic cultivars were evaluated for Onion yellow dwarf virus - OYDV and Leek yellow stripe virus - LYSV disease intensity. The presence of virus was confirmed with DAS-ELISA test. Out of 270 tested samples, 60.4% proved to be infected with at least one of viral species. OYDV was found in 60.0%, and LYSV in 3.0% of analyzed plants. In 2.6% of samples mixed contamination with both viruses was determined. Percent of infection of tested garlic cultivars varied from 0 to 100% with OYDV and 0-30% with LYSV., Tokom 2009. godine utvrđen je intenzitet zaraze 27 genotipova jesenjeg belog luka virusima žute patuljavosti crnog luka (Onion yellow dwarf virus - OYDV) i žute prugavosti praziluka (Leek yellow stripe virus - LYSV). Prisustvo virusa dokazano je pomoću DAS -ELISA testa. Od 270 testiranih uzoraka u 60,4% je utvrđena zaraza sa bar jednim od dva ispitivana virusa. OYDV je bio utvrđen u 60,0%, a LYSV u 3,0% analiziranih biljaka. U 2,6 % uzoraka utvrđena je mešovita zaraza sa oba virusa. Zaraženost ispitivanih genotipova jesenjeg belog luka sa OYDV se kretala u granicama 0-100%, a sa LYSV 0-30%.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Incidence of onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYMV) on garlic, Intenzitet zaraze belog luka virusom žute patuljavosti crnog luka (OYDV) i virusom žute prugavosti praziluka (LYMV)",
pages = "393-389",
number = "4",
volume = "39",
url = "https://hdl.handle.net/21.15107/rcub_fiver_940"
}

Bagi, F., Gvozdanović-Varga, J., Budakov, D., Stojšin, V., Janićijević, M., Šantić, M.,& Jasnić, S.. (2011). Incidence of onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYMV) on garlic. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 39(4), 389-393.
https://hdl.handle.net/21.15107/rcub_fiver_940

Bagi F, Gvozdanović-Varga J, Budakov D, Stojšin V, Janićijević M, Šantić M, Jasnić S. Incidence of onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYMV) on garlic. in Biljni lekar. 2011;39(4):389-393.
https://hdl.handle.net/21.15107/rcub_fiver_940 .

Bagi, Ferenc, Gvozdanović-Varga, Jelica, Budakov, Dragana, Stojšin, Vera, Janićijević, Milana, Šantić, Marinela, Jasnić, Stevan, "Incidence of onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYMV) on garlic" in Biljni lekar, 39, no. 4 (2011):389-393,
https://hdl.handle.net/21.15107/rcub_fiver_940 .

TY  - CONF
AU  - Kiprovski, Biljana
AU  - Malenčić, Djordje
AU  - Popović, Milan
AU  - Stojšin, Vera
AU  - Budakov, Dragana
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/2238
AB  - Changes in enzymatic and non-enzymatic antioxidant systems in leaves and roots of soybean and maize seedlings were studied after inoculation with Rhizoctonia solani Kühn, AG 2-2 IIIB. After the infection with R. solani, increase in superoxide anion (O2 .- ) scavenging activity was recorded only in leaves and roots of soybean plants. Hydroxyl radical (.OH), the production of which was remarkably increased in infected soybean seedlings, were invariably produced in infected maize seedlings in comparison to control. Furthermore, superoxide-dismutase activity increased only in infected maize roots, which could point to enhanced O2 .-dismutation. Nevertheless, lipid peroxidation intensity was notably recorded only in soybean plants, which could lead to presumption that .OH was successfully neutralized in maize seedlings and that membrane lipids were prevented from peroxidation with .OH, potentially the most responsible for these processes. Peroxidase were significantly active in infected leaves compared to control in both species, especially in soybean. Naturally, ROS accumulation induced significantly enhanced phenylalanin ammonialyase (PAL) activity, which escalated phenolic compounds synthesis in maize seedlings. Ability to accumulate phenolics, under the condition of pathogen attack, correlated with the results for the DPPH-assay that determines these maize plants in active group1.
PB  - The Society for Free Radical Research-Europe (SFRR-E)
C3  - Book of Abstracts, 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants, 05-08.11.2011., Budapest, Hungary, 250, Poster 158
T1  - Changes in antioxidant capacity of soybean and maize seedlings as a response to Rhizoctonia solani Kühn infection
EP  - 250
SP  - 250/ Poster 158
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2238
ER  - 

@conference{
author = "Kiprovski, Biljana and Malenčić, Djordje and Popović, Milan and Stojšin, Vera and Budakov, Dragana",
year = "2011",
abstract = "Changes in enzymatic and non-enzymatic antioxidant systems in leaves and roots of soybean and maize seedlings were studied after inoculation with Rhizoctonia solani Kühn, AG 2-2 IIIB. After the infection with R. solani, increase in superoxide anion (O2 .- ) scavenging activity was recorded only in leaves and roots of soybean plants. Hydroxyl radical (.OH), the production of which was remarkably increased in infected soybean seedlings, were invariably produced in infected maize seedlings in comparison to control. Furthermore, superoxide-dismutase activity increased only in infected maize roots, which could point to enhanced O2 .-dismutation. Nevertheless, lipid peroxidation intensity was notably recorded only in soybean plants, which could lead to presumption that .OH was successfully neutralized in maize seedlings and that membrane lipids were prevented from peroxidation with .OH, potentially the most responsible for these processes. Peroxidase were significantly active in infected leaves compared to control in both species, especially in soybean. Naturally, ROS accumulation induced significantly enhanced phenylalanin ammonialyase (PAL) activity, which escalated phenolic compounds synthesis in maize seedlings. Ability to accumulate phenolics, under the condition of pathogen attack, correlated with the results for the DPPH-assay that determines these maize plants in active group1.",
publisher = "The Society for Free Radical Research-Europe (SFRR-E)",
journal = "Book of Abstracts, 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants, 05-08.11.2011., Budapest, Hungary, 250, Poster 158",
title = "Changes in antioxidant capacity of soybean and maize seedlings as a response to Rhizoctonia solani Kühn infection",
pages = "250-250/ Poster 158",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2238"
}

Kiprovski, B., Malenčić, D., Popović, M., Stojšin, V.,& Budakov, D.. (2011). Changes in antioxidant capacity of soybean and maize seedlings as a response to Rhizoctonia solani Kühn infection. in Book of Abstracts, 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants, 05-08.11.2011., Budapest, Hungary, 250, Poster 158
The Society for Free Radical Research-Europe (SFRR-E)., 250/ Poster 158-250.
https://hdl.handle.net/21.15107/rcub_fiver_2238

Kiprovski B, Malenčić D, Popović M, Stojšin V, Budakov D. Changes in antioxidant capacity of soybean and maize seedlings as a response to Rhizoctonia solani Kühn infection. in Book of Abstracts, 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants, 05-08.11.2011., Budapest, Hungary, 250, Poster 158. 2011;:250/ Poster 158-250.
https://hdl.handle.net/21.15107/rcub_fiver_2238 .

Kiprovski, Biljana, Malenčić, Djordje, Popović, Milan, Stojšin, Vera, Budakov, Dragana, "Changes in antioxidant capacity of soybean and maize seedlings as a response to Rhizoctonia solani Kühn infection" in Book of Abstracts, 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants, 05-08.11.2011., Budapest, Hungary, 250, Poster 158 (2011):250/ Poster 158-250,
https://hdl.handle.net/21.15107/rcub_fiver_2238 .