Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol
Само за регистроване кориснике
2014
Аутори
Budakov, DraganaNagl, Nevena
Stojšin, Vera
Bagi, Ferenc
Danojević, Dario
Neher, Oliver T.
Taški-Ajduković, Ksenija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cercospora beticola, causal agent of Cercospora leaf spot (CLS) of sugar beet, is primarily controlled by fungicides. Benzimidazole and demethylation inhibiting fungicides, including carbendazim and flutriafol, have been widely used in Serbia. Since these fungicide groups have a site-specific mode of action, there is a high risk for developing resistance in target organisms, which is the most important limiting factor in Cercospora leaf spot chemical control. A rapid identification of flutriafol and carbendazim resistance can help researchers in examining the potential of different fungicide resistance management practices, as well as in selection of fungicides for use in the areas where resistance has occurred. One hundred single-conidia isolates were collected from 70 representative locations of the sugar beet production region in Serbia. Evaluation of the isolates' sensitivity was based on the reduction of mycelial growth on medium amended with 1.25 mu g mL(-1) flutriafol and 5 mu g... mL(-1) carbendazim. Resistance to flutriafol and carbendazim was detected in 16% and 96% of the tested isolates, respectively. All isolates resistant to flutriafol were resistant to carbendazim as well, which is the first report of a double resistance to fungicides in C. beticola. Detection of the isolates resistant to flutriafol and carbendazim using Cleaved Amplified Polymorphic Sequence (CAPS) markers confirmed the results of the in vitro tests. The efficacy of carbendazim, flutriafol, azoxystrobin, and tetraconazole at commercially recommended doses was evaluated in field trials where sugar beet plants in plots were inoculated with a mixture of isolates either sensitive and/or resistant to flutriafol and carbendazim. Carbendazim and flutriafol efficacy was very low in plots inoculated with isolates resistant to these fungicides. Presented results will contribute to development of a pathogen population sensitivity monitoring strategy that could be used for an effective CLS management in the region.
Кључне речи:
Cercospora leaf spot (CLS) / Fungicide resistance / Carbendazim / Flutriafol / CAPS / Fungicide efficacyИзвор:
Crop Protection, 2014, 66, 120-126Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
- Побољшање линија, хибрида и технологије гајења шећерне репе (RS-MESTD-Technological Development (TD or TR)-31015)
DOI: 10.1016/j.cropro.2014.09.010
ISSN: 0261-2194
WoS: 000345479300019
Scopus: 2-s2.0-84907864689
Колекције
Институција/група
FiVeRTY - JOUR AU - Budakov, Dragana AU - Nagl, Nevena AU - Stojšin, Vera AU - Bagi, Ferenc AU - Danojević, Dario AU - Neher, Oliver T. AU - Taški-Ajduković, Ksenija PY - 2014 UR - http://fiver.ifvcns.rs/handle/123456789/1384 AB - Cercospora beticola, causal agent of Cercospora leaf spot (CLS) of sugar beet, is primarily controlled by fungicides. Benzimidazole and demethylation inhibiting fungicides, including carbendazim and flutriafol, have been widely used in Serbia. Since these fungicide groups have a site-specific mode of action, there is a high risk for developing resistance in target organisms, which is the most important limiting factor in Cercospora leaf spot chemical control. A rapid identification of flutriafol and carbendazim resistance can help researchers in examining the potential of different fungicide resistance management practices, as well as in selection of fungicides for use in the areas where resistance has occurred. One hundred single-conidia isolates were collected from 70 representative locations of the sugar beet production region in Serbia. Evaluation of the isolates' sensitivity was based on the reduction of mycelial growth on medium amended with 1.25 mu g mL(-1) flutriafol and 5 mu g mL(-1) carbendazim. Resistance to flutriafol and carbendazim was detected in 16% and 96% of the tested isolates, respectively. All isolates resistant to flutriafol were resistant to carbendazim as well, which is the first report of a double resistance to fungicides in C. beticola. Detection of the isolates resistant to flutriafol and carbendazim using Cleaved Amplified Polymorphic Sequence (CAPS) markers confirmed the results of the in vitro tests. The efficacy of carbendazim, flutriafol, azoxystrobin, and tetraconazole at commercially recommended doses was evaluated in field trials where sugar beet plants in plots were inoculated with a mixture of isolates either sensitive and/or resistant to flutriafol and carbendazim. Carbendazim and flutriafol efficacy was very low in plots inoculated with isolates resistant to these fungicides. Presented results will contribute to development of a pathogen population sensitivity monitoring strategy that could be used for an effective CLS management in the region. PB - Elsevier Sci Ltd, Oxford T2 - Crop Protection T1 - Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol EP - 126 SP - 120 VL - 66 DO - 10.1016/j.cropro.2014.09.010 ER -
@article{ author = "Budakov, Dragana and Nagl, Nevena and Stojšin, Vera and Bagi, Ferenc and Danojević, Dario and Neher, Oliver T. and Taški-Ajduković, Ksenija", year = "2014", abstract = "Cercospora beticola, causal agent of Cercospora leaf spot (CLS) of sugar beet, is primarily controlled by fungicides. Benzimidazole and demethylation inhibiting fungicides, including carbendazim and flutriafol, have been widely used in Serbia. Since these fungicide groups have a site-specific mode of action, there is a high risk for developing resistance in target organisms, which is the most important limiting factor in Cercospora leaf spot chemical control. A rapid identification of flutriafol and carbendazim resistance can help researchers in examining the potential of different fungicide resistance management practices, as well as in selection of fungicides for use in the areas where resistance has occurred. One hundred single-conidia isolates were collected from 70 representative locations of the sugar beet production region in Serbia. Evaluation of the isolates' sensitivity was based on the reduction of mycelial growth on medium amended with 1.25 mu g mL(-1) flutriafol and 5 mu g mL(-1) carbendazim. Resistance to flutriafol and carbendazim was detected in 16% and 96% of the tested isolates, respectively. All isolates resistant to flutriafol were resistant to carbendazim as well, which is the first report of a double resistance to fungicides in C. beticola. Detection of the isolates resistant to flutriafol and carbendazim using Cleaved Amplified Polymorphic Sequence (CAPS) markers confirmed the results of the in vitro tests. The efficacy of carbendazim, flutriafol, azoxystrobin, and tetraconazole at commercially recommended doses was evaluated in field trials where sugar beet plants in plots were inoculated with a mixture of isolates either sensitive and/or resistant to flutriafol and carbendazim. Carbendazim and flutriafol efficacy was very low in plots inoculated with isolates resistant to these fungicides. Presented results will contribute to development of a pathogen population sensitivity monitoring strategy that could be used for an effective CLS management in the region.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Crop Protection", title = "Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol", pages = "126-120", volume = "66", doi = "10.1016/j.cropro.2014.09.010" }
Budakov, D., Nagl, N., Stojšin, V., Bagi, F., Danojević, D., Neher, O. T.,& Taški-Ajduković, K.. (2014). Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol. in Crop Protection Elsevier Sci Ltd, Oxford., 66, 120-126. https://doi.org/10.1016/j.cropro.2014.09.010
Budakov D, Nagl N, Stojšin V, Bagi F, Danojević D, Neher OT, Taški-Ajduković K. Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol. in Crop Protection. 2014;66:120-126. doi:10.1016/j.cropro.2014.09.010 .
Budakov, Dragana, Nagl, Nevena, Stojšin, Vera, Bagi, Ferenc, Danojević, Dario, Neher, Oliver T., Taški-Ajduković, Ksenija, "Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol" in Crop Protection, 66 (2014):120-126, https://doi.org/10.1016/j.cropro.2014.09.010 . .