TY  - JOUR
AU  - Stankov Petreš, Aleksandra
AU  - Stojšin, Vera
AU  - Nagl, Nevena
AU  - Petreš, Mladen
AU  - Grahovac, Mila
AU  - Ćurčić, Živko
AU  - Budakov, Dragana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3344
AB  - Sugar beet is one of the most profitable crops in Serbia. The charcoal root rot is an emerging disease of sugar beet that significantly reduces yield and affects the economic viability of production. The objectives of this research were to determine the antagonist activity of selected Trichoderma harzianum isolates (T1 to T13) on two Macrophomina phaseolina isolates in vitro using a dual culture test and a volatile compounds test, as well as in vivo. The efficacy of these treatments was compared to two commercial biopesticides (Bacillomix® Original and Trifender Pro®) and one synthetic fungicide (Funomil 700 WG®). The strongest inhibition in the dual culture test was observed in the treatment with T. harzianum T2 against both tested M. phaseolina isolates. Also, the volatile compounds produced by isolates T2 and T12 exhibited the strongest inhibitory effect on M. phaseolina isolates (from 48.22 to 62.75%). Differences in efficacy were found not only between tested Trichoderma isolates, but also between M. phaseolina isolates used for inoculation which indicates different susceptibility of pathogen population to bioagent. The in vivo test confirmed the strong antagonistic effect of the T. harzianum T2 and T12 isolates. Bacillomix® Original, Trifender Pro® and Funomil® 700 WG did not express satisfactory effects in pathogen control.
PB  - Springer
T2  - Journal of Plant Diseases and Protection
T1  - Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet
DO  - 10.1007/s41348-023-00716-w
ER  - 

@article{
author = "Stankov Petreš, Aleksandra and Stojšin, Vera and Nagl, Nevena and Petreš, Mladen and Grahovac, Mila and Ćurčić, Živko and Budakov, Dragana",
year = "2023",
abstract = "Sugar beet is one of the most profitable crops in Serbia. The charcoal root rot is an emerging disease of sugar beet that significantly reduces yield and affects the economic viability of production. The objectives of this research were to determine the antagonist activity of selected Trichoderma harzianum isolates (T1 to T13) on two Macrophomina phaseolina isolates in vitro using a dual culture test and a volatile compounds test, as well as in vivo. The efficacy of these treatments was compared to two commercial biopesticides (Bacillomix® Original and Trifender Pro®) and one synthetic fungicide (Funomil 700 WG®). The strongest inhibition in the dual culture test was observed in the treatment with T. harzianum T2 against both tested M. phaseolina isolates. Also, the volatile compounds produced by isolates T2 and T12 exhibited the strongest inhibitory effect on M. phaseolina isolates (from 48.22 to 62.75%). Differences in efficacy were found not only between tested Trichoderma isolates, but also between M. phaseolina isolates used for inoculation which indicates different susceptibility of pathogen population to bioagent. The in vivo test confirmed the strong antagonistic effect of the T. harzianum T2 and T12 isolates. Bacillomix® Original, Trifender Pro® and Funomil® 700 WG did not express satisfactory effects in pathogen control.",
publisher = "Springer",
journal = "Journal of Plant Diseases and Protection",
title = "Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet",
doi = "10.1007/s41348-023-00716-w"
}

Stankov Petreš, A., Stojšin, V., Nagl, N., Petreš, M., Grahovac, M., Ćurčić, Ž.,& Budakov, D.. (2023). Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet. in Journal of Plant Diseases and Protection
Springer..
https://doi.org/10.1007/s41348-023-00716-w

Stankov Petreš A, Stojšin V, Nagl N, Petreš M, Grahovac M, Ćurčić Ž, Budakov D. Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet. in Journal of Plant Diseases and Protection. 2023;.
doi:10.1007/s41348-023-00716-w .

Stankov Petreš, Aleksandra, Stojšin, Vera, Nagl, Nevena, Petreš, Mladen, Grahovac, Mila, Ćurčić, Živko, Budakov, Dragana, "Trichoderma harzianum as a potential biological agent in control of the charcoal root rot of sugar beet" in Journal of Plant Diseases and Protection (2023),
https://doi.org/10.1007/s41348-023-00716-w . .

TY  - CONF
AU  - Loc, Marta
AU  - Grahovac, Mila
AU  - Aćimović, Milica
AU  - Petreš, Mladen
AU  - Budakov, Dragana
AU  - Dudaš, Tatjana
AU  - Stojšin, Vera
AU  - Milošević, Dragana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3365
AB  - Vrste roda Pectobacterium su proteklih godina postale jedne od deset najproučavanijih fitopatogenih bakterija. Pažnju zavređuju zbog sve učestalije pojave novih vrsta, ekstremnoj adaptivnosti različitim domaćinima i uslovima životne sredine. Intenzivna trgovinska razmena dovodi do povećanja mogućnosti dospevanja novih vrsta na udaljena područja proizvodnje krompira, uzimajući u obzir da je glavni izvor inokuluma zaražen vegetativni materijal namenjen za sadnju. Štete uzrokovane vrstama Pectobacterium dosežu velike razmere, s obzirom na to da svoju aktivnost nastavljaju i po završetku vegetacije – prilikom transporta, prerade i u uslovima skladišta. Suzbijanje ovih patogena postaje ekstremno zahtevno uzimajući u obzir opšti nedostatak baktericida, te su mnogobrojni napori učinjeni sa ciljem iznalaženja načina suzbijanja prouzrokovača vlažne truleži krtola u uslovima skladišta. Mnoga istraživanja ukazuju na visoku efikasnost biljnih ekstrakata i etarskih ulja, kao moćnih agenasa zaštite. U cilju utvrđivanja efekata etarskih ulja (EU) bosiljka (Ocimum basilicum), čubra (Satureja hortensis), divljeg origana (Origanum vulgare), vreska (Calluna vulgaris) i timjana (Thymus vulgaris) na vrste P. carotovorum (Pc), P. brasiliense (Pb) i P. punjabense (Pp), sproveden je ogled in planta, na krtolama krompira sorte Lady Claire, jedne od najosetljivijih sorti na Pectobacterium spp.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.
T1  - Etarska ulja u suzbijanju prouzrokovača vlažne truleži krtola krompira iz roda Pectobacterium
EP  - 44
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3365
ER  - 

@conference{
author = "Loc, Marta and Grahovac, Mila and Aćimović, Milica and Petreš, Mladen and Budakov, Dragana and Dudaš, Tatjana and Stojšin, Vera and Milošević, Dragana",
year = "2022",
abstract = "Vrste roda Pectobacterium su proteklih godina postale jedne od deset najproučavanijih fitopatogenih bakterija. Pažnju zavređuju zbog sve učestalije pojave novih vrsta, ekstremnoj adaptivnosti različitim domaćinima i uslovima životne sredine. Intenzivna trgovinska razmena dovodi do povećanja mogućnosti dospevanja novih vrsta na udaljena područja proizvodnje krompira, uzimajući u obzir da je glavni izvor inokuluma zaražen vegetativni materijal namenjen za sadnju. Štete uzrokovane vrstama Pectobacterium dosežu velike razmere, s obzirom na to da svoju aktivnost nastavljaju i po završetku vegetacije – prilikom transporta, prerade i u uslovima skladišta. Suzbijanje ovih patogena postaje ekstremno zahtevno uzimajući u obzir opšti nedostatak baktericida, te su mnogobrojni napori učinjeni sa ciljem iznalaženja načina suzbijanja prouzrokovača vlažne truleži krtola u uslovima skladišta. Mnoga istraživanja ukazuju na visoku efikasnost biljnih ekstrakata i etarskih ulja, kao moćnih agenasa zaštite. U cilju utvrđivanja efekata etarskih ulja (EU) bosiljka (Ocimum basilicum), čubra (Satureja hortensis), divljeg origana (Origanum vulgare), vreska (Calluna vulgaris) i timjana (Thymus vulgaris) na vrste P. carotovorum (Pc), P. brasiliense (Pb) i P. punjabense (Pp), sproveden je ogled in planta, na krtolama krompira sorte Lady Claire, jedne od najosetljivijih sorti na Pectobacterium spp.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.",
title = "Etarska ulja u suzbijanju prouzrokovača vlažne truleži krtola krompira iz roda Pectobacterium",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3365"
}

Loc, M., Grahovac, M., Aćimović, M., Petreš, M., Budakov, D., Dudaš, T., Stojšin, V.,& Milošević, D.. (2022). Etarska ulja u suzbijanju prouzrokovača vlažne truleži krtola krompira iz roda Pectobacterium. in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.
Beograd : Društvo za zaštitu bilja Srbije., 44-44.
https://hdl.handle.net/21.15107/rcub_fiver_3365

Loc M, Grahovac M, Aćimović M, Petreš M, Budakov D, Dudaš T, Stojšin V, Milošević D. Etarska ulja u suzbijanju prouzrokovača vlažne truleži krtola krompira iz roda Pectobacterium. in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.. 2022;:44-44.
https://hdl.handle.net/21.15107/rcub_fiver_3365 .

Loc, Marta, Grahovac, Mila, Aćimović, Milica, Petreš, Mladen, Budakov, Dragana, Dudaš, Tatjana, Stojšin, Vera, Milošević, Dragana, "Etarska ulja u suzbijanju prouzrokovača vlažne truleži krtola krompira iz roda Pectobacterium" in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022. (2022):44-44,
https://hdl.handle.net/21.15107/rcub_fiver_3365 .

TY  - CONF
AU  - Petreš, Mladen
AU  - Aćimović, Milica
AU  - Loc, Marta
AU  - Dudaš, Tatjana
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Grahovac, Mila
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3369
AB  - Vrste roda Colletotrichum, prouzrokovači gorke truleži jabuke, predstavljaju jedne od najznačajnijih prouzrokovača truleži plodova jabuke u skladištu. Zbog dominantne zastupljenosti u odnosu na druge vrste ovog roda posebno su značajne vrste C. acutatum i C. gloeosporioides. S obzirom da upotreba fungicida nakon berbe i tokom skladištenja nije dozvoljena zbog njihovih nepovoljnih ekotoksikoloških svojstava, a plodovi ostaju nezaštićeni tokom skladištenja, neophodno je definisanje alternativnih rešenja. Prethodna istraživanja su dokazala značajan antifungalni potencijal etarskih ulja i njihovih hidrolata (sporednih proizvoda koji se dobijaju pri proizvodnji etarskih ulja), te mogu predstavljati adekvatnu zamenu fungicidima. U ogledu je ispitan uticaj etarskih ulja čubra (Satureja hortensis), bosiljka (Ocimum basilicum), muskatne žalfije (Salvia sclarea) i divljeg origana (Origanum vulgare), kao i hidrolata etarskih ulja čubra, bosiljka i muskatne žalfije na izolate KA7 (C. acutatum) i MRMCD6 (C. gloeosporioides).
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.
T1  - Uticaj etarskih ulja i hidrolata etarskih ulja na razvoj prouzrokovača gorke truleži jabuke
EP  - 39
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3369
ER  - 

@conference{
author = "Petreš, Mladen and Aćimović, Milica and Loc, Marta and Dudaš, Tatjana and Budakov, Dragana and Stojšin, Vera and Grahovac, Mila",
year = "2022",
abstract = "Vrste roda Colletotrichum, prouzrokovači gorke truleži jabuke, predstavljaju jedne od najznačajnijih prouzrokovača truleži plodova jabuke u skladištu. Zbog dominantne zastupljenosti u odnosu na druge vrste ovog roda posebno su značajne vrste C. acutatum i C. gloeosporioides. S obzirom da upotreba fungicida nakon berbe i tokom skladištenja nije dozvoljena zbog njihovih nepovoljnih ekotoksikoloških svojstava, a plodovi ostaju nezaštićeni tokom skladištenja, neophodno je definisanje alternativnih rešenja. Prethodna istraživanja su dokazala značajan antifungalni potencijal etarskih ulja i njihovih hidrolata (sporednih proizvoda koji se dobijaju pri proizvodnji etarskih ulja), te mogu predstavljati adekvatnu zamenu fungicidima. U ogledu je ispitan uticaj etarskih ulja čubra (Satureja hortensis), bosiljka (Ocimum basilicum), muskatne žalfije (Salvia sclarea) i divljeg origana (Origanum vulgare), kao i hidrolata etarskih ulja čubra, bosiljka i muskatne žalfije na izolate KA7 (C. acutatum) i MRMCD6 (C. gloeosporioides).",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.",
title = "Uticaj etarskih ulja i hidrolata etarskih ulja na razvoj prouzrokovača gorke truleži jabuke",
pages = "39-38",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3369"
}

Petreš, M., Aćimović, M., Loc, M., Dudaš, T., Budakov, D., Stojšin, V.,& Grahovac, M.. (2022). Uticaj etarskih ulja i hidrolata etarskih ulja na razvoj prouzrokovača gorke truleži jabuke. in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.
Beograd : Društvo za zaštitu bilja Srbije., 38-39.
https://hdl.handle.net/21.15107/rcub_fiver_3369

Petreš M, Aćimović M, Loc M, Dudaš T, Budakov D, Stojšin V, Grahovac M. Uticaj etarskih ulja i hidrolata etarskih ulja na razvoj prouzrokovača gorke truleži jabuke. in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022.. 2022;:38-39.
https://hdl.handle.net/21.15107/rcub_fiver_3369 .

Petreš, Mladen, Aćimović, Milica, Loc, Marta, Dudaš, Tatjana, Budakov, Dragana, Stojšin, Vera, Grahovac, Mila, "Uticaj etarskih ulja i hidrolata etarskih ulja na razvoj prouzrokovača gorke truleži jabuke" in Zbornik rezimea radova, 17. Savetovanje o zaštiti bilja, Zlatibor, 28. novembar - 1. decembar 2022. (2022):38-39,
https://hdl.handle.net/21.15107/rcub_fiver_3369 .

TY  - CONF
AU  - Grahovac, Mila
AU  - Aćimović, Milica
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Loc, Marta
AU  - Petreš, Mladen
AU  - Dudaš, Tatjana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3102
AB  - Under conditions of intensive plant production and intensive international trade with plant material, coupled with climate changes, plant pathogens became one of the major limiting factors in food and feed production. Besides these extremely favorable conditions for reproduction and dissemination of plant pathogens, resistance development to available control tools and ecotoxicological properties of these tools show that plant pathogen control has never been more challenging. Development of alternative, eco-friendly control tools against phytopathogens, became a necessity of contemporary agriculture. Essential oils are mixtures of complex volatile compounds. They are synthesized through secondary metabolic pathways in many plant species. Essential oils are normally extracted by hydro-distillation or steam distillation of plant tissues. Hydrolates are co-products of the distillation formed in significantly higher quantities than the target substance. Hydrolate represents aromatic water containing approximately 0.1% essential oil compounds.
PB  - Belgrade : Serbian Plant Physiology Society
PB  - Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade
T1  - Essential oils and hydrolates in control of plant pathogens
EP  - 91
SP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3102
ER  - 

@conference{
author = "Grahovac, Mila and Aćimović, Milica and Budakov, Dragana and Stojšin, Vera and Loc, Marta and Petreš, Mladen and Dudaš, Tatjana",
year = "2022",
abstract = "Under conditions of intensive plant production and intensive international trade with plant material, coupled with climate changes, plant pathogens became one of the major limiting factors in food and feed production. Besides these extremely favorable conditions for reproduction and dissemination of plant pathogens, resistance development to available control tools and ecotoxicological properties of these tools show that plant pathogen control has never been more challenging. Development of alternative, eco-friendly control tools against phytopathogens, became a necessity of contemporary agriculture. Essential oils are mixtures of complex volatile compounds. They are synthesized through secondary metabolic pathways in many plant species. Essential oils are normally extracted by hydro-distillation or steam distillation of plant tissues. Hydrolates are co-products of the distillation formed in significantly higher quantities than the target substance. Hydrolate represents aromatic water containing approximately 0.1% essential oil compounds.",
publisher = "Belgrade : Serbian Plant Physiology Society, Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“, Belgrade : University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade",
title = "Essential oils and hydrolates in control of plant pathogens",
pages = "91-91",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3102"
}

Grahovac, M., Aćimović, M., Budakov, D., Stojšin, V., Loc, M., Petreš, M.,& Dudaš, T.. (2022). Essential oils and hydrolates in control of plant pathogens. in Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade
Belgrade : Serbian Plant Physiology Society., 91-91.
https://hdl.handle.net/21.15107/rcub_fiver_3102

Grahovac M, Aćimović M, Budakov D, Stojšin V, Loc M, Petreš M, Dudaš T. Essential oils and hydrolates in control of plant pathogens. in Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade. 2022;:91-91.
https://hdl.handle.net/21.15107/rcub_fiver_3102 .

Grahovac, Mila, Aćimović, Milica, Budakov, Dragana, Stojšin, Vera, Loc, Marta, Petreš, Mladen, Dudaš, Tatjana, "Essential oils and hydrolates in control of plant pathogens" in Book of Abstracts, 4th International Conference on Plant Biology (23rd SPPS Meeting), 6-8 October 2022, Belgrade (2022):91-91,
https://hdl.handle.net/21.15107/rcub_fiver_3102 .

TY  - JOUR
AU  - Vukotić, Jelena
AU  - Stojšin, Vera
AU  - Nagl, Nevena
AU  - Petreš, Mladen
AU  - Hrustić, jovana
AU  - Grahovac, Mila
AU  - Tanović, Boris
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3984
AB  - Neofabraea spp. is one of the major causal agents of the postharvest decay of apple fruit.
Bull’s eye rot can cause fruit rot, as well as tree canker. In Serbia, during February and March, 2017 and 2018, 56.3% of collected stored rotten apple fruits were found to be infected with Neofabraea spp. Using polymerase chain reaction, phylogenetic analysis, and morphological characterization, Neofabraea alba was identified as the causal agent of Bull’s eye rot. Twenty-five selected isolates were analyzed by DNA sequencing of the internal transcribed spacer and 16 S mitochondrial ribosomal RNA. The phylogenetic analyses provided information on the distance between Neofabraea spp., while merged phylogenetic tree was required to give insight and clarify the relationships between isolates of Neofabraea alba. N. alba demonstrated pathogenicity on apple fruit as well as on branches, with significantly different aggressiveness among the isolates. Isolates were able to produce larger cankers and acervuli on apple branches with abundant sporulation All identified isolates were separated into two morphotypes and described based on their macromorphological characteristics on culture media. It was noticed that different culture media, pH, and temperature could affect macromorphological characteristics and development. Representative isolates were able to grow at a temperature range of 0 to 25  C, above which only one isolate was able to grow on culture media. All isolates showed growth at a pH range of 2–10, with significant differences detected between isolates at different pH values. The type of cultivation medium significantly affected the size and shape of conidia, while the presence of the microconidia was recorded within isolates. The aim of this study was to identify the causal agents of Bull’s eye rot on stored apples in Serbia in the period 2017–2018 and characterize the obtained isolates based on pathogenic, morphological, physiological, and molecular criteria.
PB  - Basel : MDPI
T2  - Agronomy - Basel
T1  - Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia
IS  - 9
SP  - 2015
VL  - 12
DO  - 10.3390/agronomy12092015
ER  - 

@article{
author = "Vukotić, Jelena and Stojšin, Vera and Nagl, Nevena and Petreš, Mladen and Hrustić, jovana and Grahovac, Mila and Tanović, Boris",
year = "2022",
abstract = "Neofabraea spp. is one of the major causal agents of the postharvest decay of apple fruit.
Bull’s eye rot can cause fruit rot, as well as tree canker. In Serbia, during February and March, 2017 and 2018, 56.3% of collected stored rotten apple fruits were found to be infected with Neofabraea spp. Using polymerase chain reaction, phylogenetic analysis, and morphological characterization, Neofabraea alba was identified as the causal agent of Bull’s eye rot. Twenty-five selected isolates were analyzed by DNA sequencing of the internal transcribed spacer and 16 S mitochondrial ribosomal RNA. The phylogenetic analyses provided information on the distance between Neofabraea spp., while merged phylogenetic tree was required to give insight and clarify the relationships between isolates of Neofabraea alba. N. alba demonstrated pathogenicity on apple fruit as well as on branches, with significantly different aggressiveness among the isolates. Isolates were able to produce larger cankers and acervuli on apple branches with abundant sporulation All identified isolates were separated into two morphotypes and described based on their macromorphological characteristics on culture media. It was noticed that different culture media, pH, and temperature could affect macromorphological characteristics and development. Representative isolates were able to grow at a temperature range of 0 to 25  C, above which only one isolate was able to grow on culture media. All isolates showed growth at a pH range of 2–10, with significant differences detected between isolates at different pH values. The type of cultivation medium significantly affected the size and shape of conidia, while the presence of the microconidia was recorded within isolates. The aim of this study was to identify the causal agents of Bull’s eye rot on stored apples in Serbia in the period 2017–2018 and characterize the obtained isolates based on pathogenic, morphological, physiological, and molecular criteria.",
publisher = "Basel : MDPI",
journal = "Agronomy - Basel",
title = "Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia",
number = "9",
pages = "2015",
volume = "12",
doi = "10.3390/agronomy12092015"
}

Vukotić, J., Stojšin, V., Nagl, N., Petreš, M., Hrustić, j., Grahovac, M.,& Tanović, B.. (2022). Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia. in Agronomy - Basel
Basel : MDPI., 12(9), 2015.
https://doi.org/10.3390/agronomy12092015

Vukotić J, Stojšin V, Nagl N, Petreš M, Hrustić J, Grahovac M, Tanović B. Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia. in Agronomy - Basel. 2022;12(9):2015.
doi:10.3390/agronomy12092015 .

Vukotić, Jelena, Stojšin, Vera, Nagl, Nevena, Petreš, Mladen, Hrustić, jovana, Grahovac, Mila, Tanović, Boris, "Morphological, Molecular, and Pathogenic Characterization of Neofabraea alba, a Postharvest Pathogen of Apple in Serbia" in Agronomy - Basel, 12, no. 9 (2022):2015,
https://doi.org/10.3390/agronomy12092015 . .

TY  - JOUR
AU  - Loc, Marta
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Ivanović, Žarko
AU  - Budakov, Dragana
AU  - Grahovac, Jovana
AU  - Vlajkov, Vanja
AU  - Pajčin, Ivana
AU  - Grahovac, Mila
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2679
AB  - Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia
IS  - 5
SP  - 1513
VL  - 106
DO  - 10.1094/PDIS-06-21-1199-PDN
ER  - 

@article{
author = "Loc, Marta and Milošević, Dragana and Ignjatov, Maja and Ivanović, Žarko and Budakov, Dragana and Grahovac, Jovana and Vlajkov, Vanja and Pajčin, Ivana and Grahovac, Mila",
year = "2022",
abstract = "Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia",
number = "5",
pages = "1513",
volume = "106",
doi = "10.1094/PDIS-06-21-1199-PDN"
}

Loc, M., Milošević, D., Ignjatov, M., Ivanović, Ž., Budakov, D., Grahovac, J., Vlajkov, V., Pajčin, I.,& Grahovac, M.. (2022). First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease
American Phytopathological Society., 106(5), 1513.
https://doi.org/10.1094/PDIS-06-21-1199-PDN

Loc M, Milošević D, Ignjatov M, Ivanović Ž, Budakov D, Grahovac J, Vlajkov V, Pajčin I, Grahovac M. First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia. in Plant Disease. 2022;106(5):1513.
doi:10.1094/PDIS-06-21-1199-PDN .

Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, Grahovac, Mila, "First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia" in Plant Disease, 106, no. 5 (2022):1513,
https://doi.org/10.1094/PDIS-06-21-1199-PDN . .

TY  - CONF
AU  - Petreš, Mladen
AU  - Aćimović, Milica
AU  - Loc, Marta
AU  - Dudaš, Tatjana
AU  - Jovanović, Dimitrije
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Grahovac, Mila
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/4249
AB  - Pripadnici roda Fusarium, kao prouzrokovači fuzariozne truleži ploda jabuke, pored gubitaka koje prouzrokuju u proizvodnji jabuke, mogu sintetisati mikotoksine što je dodatni aspekt njihovog štetnog delovanja koji se ogleda u kontaminaciji plodova jabuke i proizvoda koji se dobijaju njenom preradom. Vrsta Fusarium avenaceum je u periodu 2016 - 2020. godine bila dominantni prouzrokovač fuzariozne truleži uskladištenih plodova jabuke na teritoriji R. Srbije, dok se vrsta Fusarium graminearum javljala sporadično. Za obe vrste utvrđen je kapacitet za proizvodnju mikotoksina.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.
T1  - Etarska ulja i hidrolati kao inhibitori razvoja prouzrokovača fuzariozne truleži jabuke
EP  - 65
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4249
ER  - 

@conference{
author = "Petreš, Mladen and Aćimović, Milica and Loc, Marta and Dudaš, Tatjana and Jovanović, Dimitrije and Budakov, Dragana and Stojšin, Vera and Grahovac, Mila",
year = "2021",
abstract = "Pripadnici roda Fusarium, kao prouzrokovači fuzariozne truleži ploda jabuke, pored gubitaka koje prouzrokuju u proizvodnji jabuke, mogu sintetisati mikotoksine što je dodatni aspekt njihovog štetnog delovanja koji se ogleda u kontaminaciji plodova jabuke i proizvoda koji se dobijaju njenom preradom. Vrsta Fusarium avenaceum je u periodu 2016 - 2020. godine bila dominantni prouzrokovač fuzariozne truleži uskladištenih plodova jabuke na teritoriji R. Srbije, dok se vrsta Fusarium graminearum javljala sporadično. Za obe vrste utvrđen je kapacitet za proizvodnju mikotoksina.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.",
title = "Etarska ulja i hidrolati kao inhibitori razvoja prouzrokovača fuzariozne truleži jabuke",
pages = "65-64",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4249"
}

Petreš, M., Aćimović, M., Loc, M., Dudaš, T., Jovanović, D., Budakov, D., Stojšin, V.,& Grahovac, M.. (2021). Etarska ulja i hidrolati kao inhibitori razvoja prouzrokovača fuzariozne truleži jabuke. in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.
Beograd : Društvo za zaštitu bilja Srbije., 64-65.
https://hdl.handle.net/21.15107/rcub_fiver_4249

Petreš M, Aćimović M, Loc M, Dudaš T, Jovanović D, Budakov D, Stojšin V, Grahovac M. Etarska ulja i hidrolati kao inhibitori razvoja prouzrokovača fuzariozne truleži jabuke. in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.. 2021;:64-65.
https://hdl.handle.net/21.15107/rcub_fiver_4249 .

Petreš, Mladen, Aćimović, Milica, Loc, Marta, Dudaš, Tatjana, Jovanović, Dimitrije, Budakov, Dragana, Stojšin, Vera, Grahovac, Mila, "Etarska ulja i hidrolati kao inhibitori razvoja prouzrokovača fuzariozne truleži jabuke" in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021. (2021):64-65,
https://hdl.handle.net/21.15107/rcub_fiver_4249 .

TY  - CONF
AU  - Budakov, Dragana
AU  - Stojšin, Vera
AU  - Ćurčić, Živko
AU  - Dudaš, Tatjana
AU  - Stankov, Aleksandra
AU  - Bagi, Ferenc
AU  - Grahovac, Mila
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/4252
AB  - Cercospora beticola is economically the most important sugar beet leaf disease primarily controlled by fungicide applications. However, other factors influence its incidence as well, some of which are the choice of cultivar and plant nutrition. These factors have been put together in field trial, which tested reaction of eight sugar beet genotypes (G1-G8) and 20 variants of NPK nutrition.
PB  - International Institute of Sugar Beet Research
C3  - Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels
T1  - Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot
EP  - 72
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4252
ER  - 

@conference{
author = "Budakov, Dragana and Stojšin, Vera and Ćurčić, Živko and Dudaš, Tatjana and Stankov, Aleksandra and Bagi, Ferenc and Grahovac, Mila",
year = "2020",
abstract = "Cercospora beticola is economically the most important sugar beet leaf disease primarily controlled by fungicide applications. However, other factors influence its incidence as well, some of which are the choice of cultivar and plant nutrition. These factors have been put together in field trial, which tested reaction of eight sugar beet genotypes (G1-G8) and 20 variants of NPK nutrition.",
publisher = "International Institute of Sugar Beet Research",
journal = "Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels",
title = "Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot",
pages = "72-72",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4252"
}

Budakov, D., Stojšin, V., Ćurčić, Ž., Dudaš, T., Stankov, A., Bagi, F.,& Grahovac, M.. (2020). Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot. in Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels
International Institute of Sugar Beet Research., 72-72.
https://hdl.handle.net/21.15107/rcub_fiver_4252

Budakov D, Stojšin V, Ćurčić Ž, Dudaš T, Stankov A, Bagi F, Grahovac M. Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot. in Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels. 2020;:72-72.
https://hdl.handle.net/21.15107/rcub_fiver_4252 .

Budakov, Dragana, Stojšin, Vera, Ćurčić, Živko, Dudaš, Tatjana, Stankov, Aleksandra, Bagi, Ferenc, Grahovac, Mila, "Influence of sugar beet cultivar and NKP nutrition on Cercospora leaf spot" in Abstracts of Papers, 77th International Institute of Sugar Beet Research (IIRB) Congress "Maximising sugar beet performance in a changing climate", 11-12 February 2020, Brussels (2020):72-72,
https://hdl.handle.net/21.15107/rcub_fiver_4252 .

TY  - JOUR
AU  - Savić, Zagorka
AU  - Dudaš, Tatjana
AU  - Loc, Marta
AU  - Grahovac, Mila
AU  - Budakov, Dragana
AU  - Jajić, Igor
AU  - Krstović, Saša
AU  - Barošević, Tijana
AU  - Krska, Rudolf
AU  - Sulyok, Michael
AU  - Stojšin, Vera
AU  - Petreš, Mladen
AU  - Stankov, Aleksandra
AU  - Vukotić, Jelena
AU  - Bagi, Ferenc
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/4355
AB  - Aspergillus flavus is the main producer of aflatoxin B1, one of the most toxic contaminants
of food and feed. With global warming, climate conditions have become favourable for aflatoxin
contamination of agricultural products in several European countries, including Serbia. The
infection of maize with A. flavus, and aflatoxin synthesis can be controlled and reduced by
application of a biocontrol product based on non‐toxigenic strains of A. flavus. Biological control
relies on competition between atoxigenic and toxigenic strains. This is the most commonly used
biological control mechanism of aflatoxin contamination in maize in countries where aflatoxins
pose a significant threat. Mytoolbox Af01, a native atoxigenic A. flavus strain, was obtained from
maize grown in Serbia and used to produce a biocontrol product that was applied in irrigated and
non‐irrigated Serbian fields during 2016 and 2017. The application of this biocontrol product
reduced aflatoxin levels in maize kernels (51–83%). The biocontrol treatment had a highly
significant effect of reducing total aflatoxin contamination by 73%. This study showed that
aflatoxin contamination control in Serbian maize can be achieved through biological control
methods using atoxigenic A. flavus strains.
PB  - Basel : MDPI
T2  - Toxins
T1  - Biological Control of Aflatoxin in Maize Grown in Serbia
IS  - 3
SP  - 162
VL  - 12
DO  - 10.3390/toxins12030162
ER  - 

@article{
author = "Savić, Zagorka and Dudaš, Tatjana and Loc, Marta and Grahovac, Mila and Budakov, Dragana and Jajić, Igor and Krstović, Saša and Barošević, Tijana and Krska, Rudolf and Sulyok, Michael and Stojšin, Vera and Petreš, Mladen and Stankov, Aleksandra and Vukotić, Jelena and Bagi, Ferenc",
year = "2020",
abstract = "Aspergillus flavus is the main producer of aflatoxin B1, one of the most toxic contaminants
of food and feed. With global warming, climate conditions have become favourable for aflatoxin
contamination of agricultural products in several European countries, including Serbia. The
infection of maize with A. flavus, and aflatoxin synthesis can be controlled and reduced by
application of a biocontrol product based on non‐toxigenic strains of A. flavus. Biological control
relies on competition between atoxigenic and toxigenic strains. This is the most commonly used
biological control mechanism of aflatoxin contamination in maize in countries where aflatoxins
pose a significant threat. Mytoolbox Af01, a native atoxigenic A. flavus strain, was obtained from
maize grown in Serbia and used to produce a biocontrol product that was applied in irrigated and
non‐irrigated Serbian fields during 2016 and 2017. The application of this biocontrol product
reduced aflatoxin levels in maize kernels (51–83%). The biocontrol treatment had a highly
significant effect of reducing total aflatoxin contamination by 73%. This study showed that
aflatoxin contamination control in Serbian maize can be achieved through biological control
methods using atoxigenic A. flavus strains.",
publisher = "Basel : MDPI",
journal = "Toxins",
title = "Biological Control of Aflatoxin in Maize Grown in Serbia",
number = "3",
pages = "162",
volume = "12",
doi = "10.3390/toxins12030162"
}

Savić, Z., Dudaš, T., Loc, M., Grahovac, M., Budakov, D., Jajić, I., Krstović, S., Barošević, T., Krska, R., Sulyok, M., Stojšin, V., Petreš, M., Stankov, A., Vukotić, J.,& Bagi, F.. (2020). Biological Control of Aflatoxin in Maize Grown in Serbia. in Toxins
Basel : MDPI., 12(3), 162.
https://doi.org/10.3390/toxins12030162

Savić Z, Dudaš T, Loc M, Grahovac M, Budakov D, Jajić I, Krstović S, Barošević T, Krska R, Sulyok M, Stojšin V, Petreš M, Stankov A, Vukotić J, Bagi F. Biological Control of Aflatoxin in Maize Grown in Serbia. in Toxins. 2020;12(3):162.
doi:10.3390/toxins12030162 .

Savić, Zagorka, Dudaš, Tatjana, Loc, Marta, Grahovac, Mila, Budakov, Dragana, Jajić, Igor, Krstović, Saša, Barošević, Tijana, Krska, Rudolf, Sulyok, Michael, Stojšin, Vera, Petreš, Mladen, Stankov, Aleksandra, Vukotić, Jelena, Bagi, Ferenc, "Biological Control of Aflatoxin in Maize Grown in Serbia" in Toxins, 12, no. 3 (2020):162,
https://doi.org/10.3390/toxins12030162 . .

TY  - JOUR
AU  - Petreš, Mladen
AU  - Kalajdžić, Jelena
AU  - Milić, Biserka
AU  - Magazin, Nenad
AU  - Stankov, Aleksandra
AU  - Medić, Jelena
AU  - Grahovac, Mila
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/4357
AB  - Hot water treatments of apple fruits prior to long-term storage have a high potential as a physical measure against apple fruit
storage rot caused by plant pathogenic fungi. Fusarium species are increasingly found as causal agents of postharvest apple
fruit rot. The effect of high temperatures on casual agents of Fusarium fruit rot, F. avenaceum and F. graminearum, was tested
in this article. In in vitro assay, spore suspensions of F. avenaceum and F. graminearum were exposed to high temperatures
(ranging from 45 to 90 °C) at different duration (ranging from 30 s to 20 min) and the treatments that significantly inhibited
mycelial growth of the isolates (53 and 57 °C for 3 and 5 min) were tested in vivo by hot water treatment of inoculated apple
fruits of Fuji cultivar. The obtained results showed that the most promising hot water treatment should be between 53 and
57 °C and between 3 and 5 min of the exposure period.
PB  - Springer
T2  - Journal of Plant Diseases and Protection
T1  - Effect of hot water treatments on apple fruit rot caused by Fusarium sp.
EP  - 655
SP  - 651
VL  - 127
DO  - 10.1007/s41348-020-00302-4
ER  - 

@article{
author = "Petreš, Mladen and Kalajdžić, Jelena and Milić, Biserka and Magazin, Nenad and Stankov, Aleksandra and Medić, Jelena and Grahovac, Mila",
year = "2020",
abstract = "Hot water treatments of apple fruits prior to long-term storage have a high potential as a physical measure against apple fruit
storage rot caused by plant pathogenic fungi. Fusarium species are increasingly found as causal agents of postharvest apple
fruit rot. The effect of high temperatures on casual agents of Fusarium fruit rot, F. avenaceum and F. graminearum, was tested
in this article. In in vitro assay, spore suspensions of F. avenaceum and F. graminearum were exposed to high temperatures
(ranging from 45 to 90 °C) at different duration (ranging from 30 s to 20 min) and the treatments that significantly inhibited
mycelial growth of the isolates (53 and 57 °C for 3 and 5 min) were tested in vivo by hot water treatment of inoculated apple
fruits of Fuji cultivar. The obtained results showed that the most promising hot water treatment should be between 53 and
57 °C and between 3 and 5 min of the exposure period.",
publisher = "Springer",
journal = "Journal of Plant Diseases and Protection",
title = "Effect of hot water treatments on apple fruit rot caused by Fusarium sp.",
pages = "655-651",
volume = "127",
doi = "10.1007/s41348-020-00302-4"
}

Petreš, M., Kalajdžić, J., Milić, B., Magazin, N., Stankov, A., Medić, J.,& Grahovac, M.. (2020). Effect of hot water treatments on apple fruit rot caused by Fusarium sp.. in Journal of Plant Diseases and Protection
Springer., 127, 651-655.
https://doi.org/10.1007/s41348-020-00302-4

Petreš M, Kalajdžić J, Milić B, Magazin N, Stankov A, Medić J, Grahovac M. Effect of hot water treatments on apple fruit rot caused by Fusarium sp.. in Journal of Plant Diseases and Protection. 2020;127:651-655.
doi:10.1007/s41348-020-00302-4 .

Petreš, Mladen, Kalajdžić, Jelena, Milić, Biserka, Magazin, Nenad, Stankov, Aleksandra, Medić, Jelena, Grahovac, Mila, "Effect of hot water treatments on apple fruit rot caused by Fusarium sp." in Journal of Plant Diseases and Protection, 127 (2020):651-655,
https://doi.org/10.1007/s41348-020-00302-4 . .