TY  - JOUR
AU  - Medić-Pap, Slađana
AU  - Živanov, Dalibor
AU  - Tančić-Živanov, Sonja
AU  - Pap, Predrag
AU  - Galović, Vladislava
AU  - Nagl, Nevena
AU  - Župunski, Vesna
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3177
AB  - Monitoring the health status of narrow-leaved ash tree seedlings (forest office Morović, locality Vinična) in the early spring of 2015, after the catastrophic floods in May 2014, revealed presence of large dark necrotic areas on 1–2 year old sprouts. The isolation of the fungal pathogen was done by standard phytopathological protocols. Three representative isolates (K41, K42 and K78) were preliminary detected and purified by a single-spore technique for further morphological, molecular analyses and pathogenicity testing. Morphological characteristics classified the isolates as Fusarium tricinctum. Tested isolates on narrow leaved ash sprouts caused reddish brown elongated necrotic lesions averaged 20.1 mm. Two marker genes, translation elongation factor 1-alpha (TEF1-α) and internal transcribed spacer (ITS1), were used in this study. Using the Basic Local Alignment Search Tool (BLAST) searching engine, nucleotide sequences were compared to all related sequences. Alignement score resulted in 98.9% identities with F. tricinctum for isolate K78, while isolates K41 and K42 showed 94.1% and 94.3% identities with F. tricinctum complex respectively. To the best of our knowledge, this is the first report of F. tricinctum pathogen infection on flood stressed narrow-leaved ash trees in Serbia.
AB  - Poljski jasen (Fraxinus angustifolia Vahl.) je drvenasta vrsta rasprostranjena u nizijskim šumama u Evropi. Region jugozapadnog Srema gde se nalaze najvrednije šume ove vrste u Srbiji, bio je izložen katastrofalnim poplavama u maju 2014. Praćenjem zdravstvenog stanja sadnica jasena (šuma Morović, lokalitet „Vinična”) u rano proleće 2015. godine otkriveno je prisustvo velikih tamnih nekrotičnih područja na izbojcima starim 1–2 godine. Sa obolelih izbojaka, standardnim fitopatološkim postupkom, urađena je izolacija patogena. Prema morfološkim karakteristikama dobijeni izolati su determinisani kao Fusarium tricinctum. Za dalje analize su uzeta tri reprezentativna monosporna izolata (K41, K42 i K78). BLAST pretragom NCBI nukleotidne baze utvrđeno je 98,9% sličnosti TEF1-α sekvence izolata K78 (MN822227.1) sa Fusarium tricinctum i 100% poklapanja ITS sekvence ovog izolata (GenBank MK928426.1) sa izolatom F. tricinctum ZMXR6 (MT446111.1). IGS sekvence izo lata K41 i K42 (MZ749901 i MZ749902) imale su 94,1% i 94,3% sličnosti sa F. tricinctum kompleksom, na osnovu BLAST pretrage FUSARIUM-ID nukleotidne baze. Testovi patogenosti na izbojcima jasena starim godinu dana pokazali su crvenkasto smeđe izdužene nekrotične lezije prosečne dužine 20,1 mm. Patogen je reizolovan iz izbojaka sa simptomima. Prema našim saznanjima, ovo je prvi nalaz F. tricinctum na stablima poljskog jasena u Srbiji.
PB  - Novi Sad : Matica srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia
T1  - Prvi nalaz Fusarium tricinctum na poljskom jasenu (Fraxinus angustifolia Vahl.) u Srbiji
EP  - 30
SP  - 19
VL  - 142
DO  - 10.2298/ZMSPN2242019M
ER  - 

@article{
author = "Medić-Pap, Slađana and Živanov, Dalibor and Tančić-Živanov, Sonja and Pap, Predrag and Galović, Vladislava and Nagl, Nevena and Župunski, Vesna",
year = "2022",
abstract = "Monitoring the health status of narrow-leaved ash tree seedlings (forest office Morović, locality Vinična) in the early spring of 2015, after the catastrophic floods in May 2014, revealed presence of large dark necrotic areas on 1–2 year old sprouts. The isolation of the fungal pathogen was done by standard phytopathological protocols. Three representative isolates (K41, K42 and K78) were preliminary detected and purified by a single-spore technique for further morphological, molecular analyses and pathogenicity testing. Morphological characteristics classified the isolates as Fusarium tricinctum. Tested isolates on narrow leaved ash sprouts caused reddish brown elongated necrotic lesions averaged 20.1 mm. Two marker genes, translation elongation factor 1-alpha (TEF1-α) and internal transcribed spacer (ITS1), were used in this study. Using the Basic Local Alignment Search Tool (BLAST) searching engine, nucleotide sequences were compared to all related sequences. Alignement score resulted in 98.9% identities with F. tricinctum for isolate K78, while isolates K41 and K42 showed 94.1% and 94.3% identities with F. tricinctum complex respectively. To the best of our knowledge, this is the first report of F. tricinctum pathogen infection on flood stressed narrow-leaved ash trees in Serbia., Poljski jasen (Fraxinus angustifolia Vahl.) je drvenasta vrsta rasprostranjena u nizijskim šumama u Evropi. Region jugozapadnog Srema gde se nalaze najvrednije šume ove vrste u Srbiji, bio je izložen katastrofalnim poplavama u maju 2014. Praćenjem zdravstvenog stanja sadnica jasena (šuma Morović, lokalitet „Vinična”) u rano proleće 2015. godine otkriveno je prisustvo velikih tamnih nekrotičnih područja na izbojcima starim 1–2 godine. Sa obolelih izbojaka, standardnim fitopatološkim postupkom, urađena je izolacija patogena. Prema morfološkim karakteristikama dobijeni izolati su determinisani kao Fusarium tricinctum. Za dalje analize su uzeta tri reprezentativna monosporna izolata (K41, K42 i K78). BLAST pretragom NCBI nukleotidne baze utvrđeno je 98,9% sličnosti TEF1-α sekvence izolata K78 (MN822227.1) sa Fusarium tricinctum i 100% poklapanja ITS sekvence ovog izolata (GenBank MK928426.1) sa izolatom F. tricinctum ZMXR6 (MT446111.1). IGS sekvence izo lata K41 i K42 (MZ749901 i MZ749902) imale su 94,1% i 94,3% sličnosti sa F. tricinctum kompleksom, na osnovu BLAST pretrage FUSARIUM-ID nukleotidne baze. Testovi patogenosti na izbojcima jasena starim godinu dana pokazali su crvenkasto smeđe izdužene nekrotične lezije prosečne dužine 20,1 mm. Patogen je reizolovan iz izbojaka sa simptomima. Prema našim saznanjima, ovo je prvi nalaz F. tricinctum na stablima poljskog jasena u Srbiji.",
publisher = "Novi Sad : Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia, Prvi nalaz Fusarium tricinctum na poljskom jasenu (Fraxinus angustifolia Vahl.) u Srbiji",
pages = "30-19",
volume = "142",
doi = "10.2298/ZMSPN2242019M"
}

Medić-Pap, S., Živanov, D., Tančić-Živanov, S., Pap, P., Galović, V., Nagl, N.,& Župunski, V.. (2022). First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia. in Zbornik Matice srpske za prirodne nauke
Novi Sad : Matica srpska., 142, 19-30.
https://doi.org/10.2298/ZMSPN2242019M

Medić-Pap S, Živanov D, Tančić-Živanov S, Pap P, Galović V, Nagl N, Župunski V. First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia. in Zbornik Matice srpske za prirodne nauke. 2022;142:19-30.
doi:10.2298/ZMSPN2242019M .

Medić-Pap, Slađana, Živanov, Dalibor, Tančić-Živanov, Sonja, Pap, Predrag, Galović, Vladislava, Nagl, Nevena, Župunski, Vesna, "First report of Fusarium tricinctum on narrow-leaved ash (Fraxinus angustifolia Vahl.) in Serbia" in Zbornik Matice srpske za prirodne nauke, 142 (2022):19-30,
https://doi.org/10.2298/ZMSPN2242019M . .

TY  - JOUR
AU  - Zlokolica, Marija Ž.
AU  - Milošević, Mirjana
AU  - Nikolić, Zorica
AU  - Galović, Vladislava
PY  - 2002
UR  - http://fiver.ifvcns.rs/handle/123456789/137
AB  - Different genetic markers are used for estimation of breeding material, its characteristics and potential for ultimate aim - heterosis of hybrids. They also point out to the qualitative seed traits at the level of linkage with genes responsible for desirable agronomic traits. This program encompasses testing methodologies for the new seed technology. Genetic analysis of breeding material during certain phases is comprised of isozymic gene expression and degrees of their variability, but it is continued (in order to be evaluated) until determination of presence or absence of some genes existing or introduced for certain traits. Using combination of different molecular methods such as PCR, RAPD and AFLP based on polymorphism of DNA fragments, the definite aim - identification of newly created products of improvement is achieved. Testing of traits of breeding material, its genetic variability and diversity is the first stage in analysis of the maize genome. It is also the condition for determination of presence of certain genes, used for obtaining the ultimate aim - attest of identity of the genotype.
AB  - Različiti genetski markeri se koriste za tumačenje selekcionog materijala njegovih osobina i osnove za krajnji cilj - heterozis hibrida. Oni su takođe pokazatelji kvaliteta semena na nivou vezanih gena za određena, tražena agronomska svojstva. Ovaj program uključuje nove metodologije za testiranje selekcionog i semenskog materijala. Genetske analize biljaka u fazama oplemenjivanja obuhvataju izoenzimsku ekspresiju gena i stepen njihove varijabilnosti, koja se nastavlja do determinacije prisustva ili odsustva gena, odgovornih za određena svojstva. Primenjuje se kombinacija različitih genetsko-molekularnih metoda, kao što su izoenzimi PCR, RAPD, AFLP, RFLP bazirani na polimorfizmu proteina i sekvenci DNK, čiji je krajnji cilj identifikacija novostvorenih genotipova i njihovo unapređenje. Utvrđivanje osobina selekcionog materijala, njegove genetske varijabilnosti i divergentnosti je prva faza u spoznaji genoma kukuruza. To je takođe uslov za determinaciju prisustva određenih gena, potrebnu za postizanje završne faze, potvrde o identitetu genotipa.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification of genetic characteristics of maize (Zea mays L.) using genetic markers
T1  - Identifikacija genetskih osobina kukuruza (Zea mays L.) korišćenjem genetskih markera
EP  - 56
IS  - 103
SP  - 47
DO  - 10.2298/ZMSPN0201047Z
ER  - 

@article{
author = "Zlokolica, Marija Ž. and Milošević, Mirjana and Nikolić, Zorica and Galović, Vladislava",
year = "2002",
abstract = "Different genetic markers are used for estimation of breeding material, its characteristics and potential for ultimate aim - heterosis of hybrids. They also point out to the qualitative seed traits at the level of linkage with genes responsible for desirable agronomic traits. This program encompasses testing methodologies for the new seed technology. Genetic analysis of breeding material during certain phases is comprised of isozymic gene expression and degrees of their variability, but it is continued (in order to be evaluated) until determination of presence or absence of some genes existing or introduced for certain traits. Using combination of different molecular methods such as PCR, RAPD and AFLP based on polymorphism of DNA fragments, the definite aim - identification of newly created products of improvement is achieved. Testing of traits of breeding material, its genetic variability and diversity is the first stage in analysis of the maize genome. It is also the condition for determination of presence of certain genes, used for obtaining the ultimate aim - attest of identity of the genotype., Različiti genetski markeri se koriste za tumačenje selekcionog materijala njegovih osobina i osnove za krajnji cilj - heterozis hibrida. Oni su takođe pokazatelji kvaliteta semena na nivou vezanih gena za određena, tražena agronomska svojstva. Ovaj program uključuje nove metodologije za testiranje selekcionog i semenskog materijala. Genetske analize biljaka u fazama oplemenjivanja obuhvataju izoenzimsku ekspresiju gena i stepen njihove varijabilnosti, koja se nastavlja do determinacije prisustva ili odsustva gena, odgovornih za određena svojstva. Primenjuje se kombinacija različitih genetsko-molekularnih metoda, kao što su izoenzimi PCR, RAPD, AFLP, RFLP bazirani na polimorfizmu proteina i sekvenci DNK, čiji je krajnji cilj identifikacija novostvorenih genotipova i njihovo unapređenje. Utvrđivanje osobina selekcionog materijala, njegove genetske varijabilnosti i divergentnosti je prva faza u spoznaji genoma kukuruza. To je takođe uslov za determinaciju prisustva određenih gena, potrebnu za postizanje završne faze, potvrde o identitetu genotipa.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification of genetic characteristics of maize (Zea mays L.) using genetic markers, Identifikacija genetskih osobina kukuruza (Zea mays L.) korišćenjem genetskih markera",
pages = "56-47",
number = "103",
doi = "10.2298/ZMSPN0201047Z"
}

Zlokolica, M. Ž., Milošević, M., Nikolić, Z.,& Galović, V.. (2002). Identification of genetic characteristics of maize (Zea mays L.) using genetic markers. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(103), 47-56.
https://doi.org/10.2298/ZMSPN0201047Z

Zlokolica MŽ, Milošević M, Nikolić Z, Galović V. Identification of genetic characteristics of maize (Zea mays L.) using genetic markers. in Zbornik Matice srpske za prirodne nauke. 2002;(103):47-56.
doi:10.2298/ZMSPN0201047Z .

Zlokolica, Marija Ž., Milošević, Mirjana, Nikolić, Zorica, Galović, Vladislava, "Identification of genetic characteristics of maize (Zea mays L.) using genetic markers" in Zbornik Matice srpske za prirodne nauke, no. 103 (2002):47-56,
https://doi.org/10.2298/ZMSPN0201047Z . .

TY  - JOUR
AU  - Zlokolica, Marija Ž.
AU  - Milošević, Mirjana
AU  - Balešević-Tubić, Svetlana
AU  - Nikolić, Zorica
AU  - Galović, Vladislava
AU  - Vujaković, Milka
PY  - 2000
UR  - http://fiver.ifvcns.rs/handle/123456789/31
AB  - Identification of plants species and their 'fingerprinting' are based on morphological, biochemical-genetic and molecular markers. The aim of this paper was to determine genetic basis of the breeding material from its source-population to its final breeding products, the varieties and hybrids. Using combination of several methods based on morphological, protein and DNK markers, the basic traits of genotypes such as: differentiability, uniformity and stability (DUS tests) were determined. These are the components necessary for identification of seed and its quality and protection of breeders rights. Among markers the AFLP method is advantageous being based on polymorphic fragments DNA and because of that the complete coverage of the genomes was achieved. By its combination with other methods the origin of genotype was determined at the international level and in accordance with UPOV regulations.
AB  - Identifikacija biljnih vrsta, njihov fingerprinting se zasniva na morfološkim, biohemijsko-genetskim i molekularnim markerima. Cilj ovog rada je utvrđivanje genetske osnove selekcionog materijala, od njegovog izvornog-populacionog, do krajnjih proizvoda selekcije, sorti i hibrida. Kombinacijom više metoda, zasnovanih na morfološkim, proteinskim i DNK markerima, utvrđuju se osnovna svojstva posmatranih genotipova, kao što su: udaljenost, uniformnost i stabilnost (DUS testovi). Ovo su komponente neophodne za identifikaciju semena, njegovog kvaliteta i zaštitu autorskih prava. Među markerima prednost ima AFLP metoda, zasnovana na polimorfizmu fragmenata DNK, kojima se postiže velika pokrivenost genoma. Kombinacijom AFLP sa drugim metodama utvrđuje se originalnost genotipa i njegov identitet za datu vrstu, u međunarodnim razmerama, u skladu sa propisima UPOV.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Zbornik radova Instituta za ratarstvo i povrtarstvo
T1  - Identification of breeding products and protection of breeders rights
T1  - Identifikacija genotipova biljnih vrsta i zaštita autorstva
EP  - 244
IS  - 33
SP  - 235
UR  - https://hdl.handle.net/21.15107/rcub_fiver_31
ER  - 

@article{
author = "Zlokolica, Marija Ž. and Milošević, Mirjana and Balešević-Tubić, Svetlana and Nikolić, Zorica and Galović, Vladislava and Vujaković, Milka",
year = "2000",
abstract = "Identification of plants species and their 'fingerprinting' are based on morphological, biochemical-genetic and molecular markers. The aim of this paper was to determine genetic basis of the breeding material from its source-population to its final breeding products, the varieties and hybrids. Using combination of several methods based on morphological, protein and DNK markers, the basic traits of genotypes such as: differentiability, uniformity and stability (DUS tests) were determined. These are the components necessary for identification of seed and its quality and protection of breeders rights. Among markers the AFLP method is advantageous being based on polymorphic fragments DNA and because of that the complete coverage of the genomes was achieved. By its combination with other methods the origin of genotype was determined at the international level and in accordance with UPOV regulations., Identifikacija biljnih vrsta, njihov fingerprinting se zasniva na morfološkim, biohemijsko-genetskim i molekularnim markerima. Cilj ovog rada je utvrđivanje genetske osnove selekcionog materijala, od njegovog izvornog-populacionog, do krajnjih proizvoda selekcije, sorti i hibrida. Kombinacijom više metoda, zasnovanih na morfološkim, proteinskim i DNK markerima, utvrđuju se osnovna svojstva posmatranih genotipova, kao što su: udaljenost, uniformnost i stabilnost (DUS testovi). Ovo su komponente neophodne za identifikaciju semena, njegovog kvaliteta i zaštitu autorskih prava. Među markerima prednost ima AFLP metoda, zasnovana na polimorfizmu fragmenata DNK, kojima se postiže velika pokrivenost genoma. Kombinacijom AFLP sa drugim metodama utvrđuje se originalnost genotipa i njegov identitet za datu vrstu, u međunarodnim razmerama, u skladu sa propisima UPOV.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Zbornik radova Instituta za ratarstvo i povrtarstvo",
title = "Identification of breeding products and protection of breeders rights, Identifikacija genotipova biljnih vrsta i zaštita autorstva",
pages = "244-235",
number = "33",
url = "https://hdl.handle.net/21.15107/rcub_fiver_31"
}

Zlokolica, M. Ž., Milošević, M., Balešević-Tubić, S., Nikolić, Z., Galović, V.,& Vujaković, M.. (2000). Identification of breeding products and protection of breeders rights. in Zbornik radova Instituta za ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad.(33), 235-244.
https://hdl.handle.net/21.15107/rcub_fiver_31

Zlokolica MŽ, Milošević M, Balešević-Tubić S, Nikolić Z, Galović V, Vujaković M. Identification of breeding products and protection of breeders rights. in Zbornik radova Instituta za ratarstvo i povrtarstvo. 2000;(33):235-244.
https://hdl.handle.net/21.15107/rcub_fiver_31 .

Zlokolica, Marija Ž., Milošević, Mirjana, Balešević-Tubić, Svetlana, Nikolić, Zorica, Galović, Vladislava, Vujaković, Milka, "Identification of breeding products and protection of breeders rights" in Zbornik radova Instituta za ratarstvo i povrtarstvo, no. 33 (2000):235-244,
https://hdl.handle.net/21.15107/rcub_fiver_31 .