TY  - CONF
AU  - Vasiljević, Sanja
AU  - Mikulić, Mira
AU  - Cvejić, Jelena
AU  - Nikolić, Zorica
AU  - Katanski, Snežana
AU  - Mamlić, Zlatica
AU  - Uhlarik, Ana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3759
AB  - Isoflavones are one of the best studied groups of phytoestrogens. Phytoestrogens are plant compounds that can exhibit a biological effect similar to the reproductive hormone estrogen. From nutritive and pharmaceutical aspect, red clover is one of the most important sources of these compounds, and they are often used as a raw material for production of dietary supplements used for alleviation of menopausal symptoms.
The objective of this study was to determine the concentrations of isoflavones in 11 diploid (2n) and 10 tetraploid (4n) cultivars of red clover (Trifolium pratense L.) from different origine, in a whole plant and separately: in stems, leaves and flowers at the stage of full flowering. Herbage samples from whole and different parts of plant were dried at 60˚C for about 48 hours, homogenized and grounded to a particle size of Ӕ = 0.8 mm as well as mixed with 2 ml of water on 37°C. After that, HCl and ethanol are added and mixture is heated to boiling. Obtained extract are purified by solid phase extraction on HLB cartridges and analyzed on HPLC after filtration. Zorbax SB C 18 reversed phase HPLC column is used for separation of main isoflavones present in red clover: formononetin, biochanin A, genistein and daidzein. Isoflavones are identified by comparing the retention times in HPLC chromatograms and UV spectral patterns with those of standard compounds. Isoflavone concentrations are quantified by external standard method using five-point regression curves of formononetin, biochanin A, genistein, and daidzein standard compounds.
The content of total isoflavones ranged from 3.83 (Marieta -2n cultivar) to 17.11 mg g-1 DM (Margot-4n cultivar). Red clover plant parts were significantly different according to isoflavone content. The leaf was the richest source of isoflavones (6.54 mg g-1 DM), followed by flowers (1,96 mg g-1 DM) and stem (1.16 mg g-1 DM). Biochanin A and formononetin were the predominant isoflavones in leaves, while genistein was mostly present in flowers. 
Tetraploid red clover cultivars (4n) had significantly higher isoflavones content compared to diploids (2n). On average, the content of isoflavones in tetraploids (11.0 mg g-1 DM) is statistically significantly higher than in diploids (8.44 mg g-1 DM).
PB  - Olomouc:Palacký University Olomouc
C3  - Abstracts of the 35th Meeting of the EUCARPIA Fodder Crops and Amenity Grasses Section in cooperation with the EUCARPIA Festulolium Working Group, 10-14 September 2023, Brno
T1  - Isoflavones profiles of some diploid and tetraploid red clover cultivars (Trifolium pratense L.) at flowering stage
EP  - 27
SP  - 27
DO  - 10.5507/vup.23.24463414
ER  - 

@conference{
author = "Vasiljević, Sanja and Mikulić, Mira and Cvejić, Jelena and Nikolić, Zorica and Katanski, Snežana and Mamlić, Zlatica and Uhlarik, Ana",
year = "2023",
abstract = "Isoflavones are one of the best studied groups of phytoestrogens. Phytoestrogens are plant compounds that can exhibit a biological effect similar to the reproductive hormone estrogen. From nutritive and pharmaceutical aspect, red clover is one of the most important sources of these compounds, and they are often used as a raw material for production of dietary supplements used for alleviation of menopausal symptoms.
The objective of this study was to determine the concentrations of isoflavones in 11 diploid (2n) and 10 tetraploid (4n) cultivars of red clover (Trifolium pratense L.) from different origine, in a whole plant and separately: in stems, leaves and flowers at the stage of full flowering. Herbage samples from whole and different parts of plant were dried at 60˚C for about 48 hours, homogenized and grounded to a particle size of Ӕ = 0.8 mm as well as mixed with 2 ml of water on 37°C. After that, HCl and ethanol are added and mixture is heated to boiling. Obtained extract are purified by solid phase extraction on HLB cartridges and analyzed on HPLC after filtration. Zorbax SB C 18 reversed phase HPLC column is used for separation of main isoflavones present in red clover: formononetin, biochanin A, genistein and daidzein. Isoflavones are identified by comparing the retention times in HPLC chromatograms and UV spectral patterns with those of standard compounds. Isoflavone concentrations are quantified by external standard method using five-point regression curves of formononetin, biochanin A, genistein, and daidzein standard compounds.
The content of total isoflavones ranged from 3.83 (Marieta -2n cultivar) to 17.11 mg g-1 DM (Margot-4n cultivar). Red clover plant parts were significantly different according to isoflavone content. The leaf was the richest source of isoflavones (6.54 mg g-1 DM), followed by flowers (1,96 mg g-1 DM) and stem (1.16 mg g-1 DM). Biochanin A and formononetin were the predominant isoflavones in leaves, while genistein was mostly present in flowers. 
Tetraploid red clover cultivars (4n) had significantly higher isoflavones content compared to diploids (2n). On average, the content of isoflavones in tetraploids (11.0 mg g-1 DM) is statistically significantly higher than in diploids (8.44 mg g-1 DM).",
publisher = "Olomouc:Palacký University Olomouc",
journal = "Abstracts of the 35th Meeting of the EUCARPIA Fodder Crops and Amenity Grasses Section in cooperation with the EUCARPIA Festulolium Working Group, 10-14 September 2023, Brno",
title = "Isoflavones profiles of some diploid and tetraploid red clover cultivars (Trifolium pratense L.) at flowering stage",
pages = "27-27",
doi = "10.5507/vup.23.24463414"
}

Vasiljević, S., Mikulić, M., Cvejić, J., Nikolić, Z., Katanski, S., Mamlić, Z.,& Uhlarik, A.. (2023). Isoflavones profiles of some diploid and tetraploid red clover cultivars (Trifolium pratense L.) at flowering stage. in Abstracts of the 35th Meeting of the EUCARPIA Fodder Crops and Amenity Grasses Section in cooperation with the EUCARPIA Festulolium Working Group, 10-14 September 2023, Brno
Olomouc:Palacký University Olomouc., 27-27.
https://doi.org/10.5507/vup.23.24463414

Vasiljević S, Mikulić M, Cvejić J, Nikolić Z, Katanski S, Mamlić Z, Uhlarik A. Isoflavones profiles of some diploid and tetraploid red clover cultivars (Trifolium pratense L.) at flowering stage. in Abstracts of the 35th Meeting of the EUCARPIA Fodder Crops and Amenity Grasses Section in cooperation with the EUCARPIA Festulolium Working Group, 10-14 September 2023, Brno. 2023;:27-27.
doi:10.5507/vup.23.24463414 .

Vasiljević, Sanja, Mikulić, Mira, Cvejić, Jelena, Nikolić, Zorica, Katanski, Snežana, Mamlić, Zlatica, Uhlarik, Ana, "Isoflavones profiles of some diploid and tetraploid red clover cultivars (Trifolium pratense L.) at flowering stage" in Abstracts of the 35th Meeting of the EUCARPIA Fodder Crops and Amenity Grasses Section in cooperation with the EUCARPIA Festulolium Working Group, 10-14 September 2023, Brno (2023):27-27,
https://doi.org/10.5507/vup.23.24463414 . .

TY  - JOUR
AU  - Tepavčević, Vesna
AU  - Cvejić, Jelena
AU  - Posa, Mihalj
AU  - Bjelica, Artur
AU  - Miladinović, Jegor
AU  - Rizou, Myrto
AU  - Aldawoud, Turki M. S.
AU  - Galanakis, Charis M.
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2094
AB  - Isoflavones are phytoestrogens with various types of biological activity. Soybean (Glycine max (L.) Merr.) is the most abundant source of isoflavones in human nutrition. The present study aims to classify the different soybean genotypes according to their isoflavone contents. HPLC analysis of isoflavone content in 22 soybean genotypes was performed. The average content of individual isoflavones in the genotypes analyzed ranged from 2.8 mu g/g to 1069,9 mu g/g while the most abundant component among the 22 genotypes was malonyl-genistin. The majority of isoflavones had a significantly higher concentration (p  lt  0.05) in the hybrid genotypes compared to parental genotypes implying heterosis. The principal component analysis (PCA) on isoflavone content and composition, including results in 83 genotypes, was conducted. The contents of daidzein and genistein were positively correlated, which could be explained by their biosynthetic pathways. Using the PCA, analyzed genotypes divided into six coherent groups regarding their specific isoflavone content. Malonyl-genistin and malonyl-daidzin were the most abundant components. Moreover, the significance of the cultivation year on the isoflavone content in soybean was pointed out. The overall results suggested the possibility of selection of suitable soybean extracts according to the content of malonyl-genistin.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Food Composition & Analysis
T1  - Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content
VL  - 95
DO  - 10.1016/j.jfca.2020.103670
ER  - 

@article{
author = "Tepavčević, Vesna and Cvejić, Jelena and Posa, Mihalj and Bjelica, Artur and Miladinović, Jegor and Rizou, Myrto and Aldawoud, Turki M. S. and Galanakis, Charis M.",
year = "2021",
abstract = "Isoflavones are phytoestrogens with various types of biological activity. Soybean (Glycine max (L.) Merr.) is the most abundant source of isoflavones in human nutrition. The present study aims to classify the different soybean genotypes according to their isoflavone contents. HPLC analysis of isoflavone content in 22 soybean genotypes was performed. The average content of individual isoflavones in the genotypes analyzed ranged from 2.8 mu g/g to 1069,9 mu g/g while the most abundant component among the 22 genotypes was malonyl-genistin. The majority of isoflavones had a significantly higher concentration (p  lt  0.05) in the hybrid genotypes compared to parental genotypes implying heterosis. The principal component analysis (PCA) on isoflavone content and composition, including results in 83 genotypes, was conducted. The contents of daidzein and genistein were positively correlated, which could be explained by their biosynthetic pathways. Using the PCA, analyzed genotypes divided into six coherent groups regarding their specific isoflavone content. Malonyl-genistin and malonyl-daidzin were the most abundant components. Moreover, the significance of the cultivation year on the isoflavone content in soybean was pointed out. The overall results suggested the possibility of selection of suitable soybean extracts according to the content of malonyl-genistin.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Food Composition & Analysis",
title = "Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content",
volume = "95",
doi = "10.1016/j.jfca.2020.103670"
}

Tepavčević, V., Cvejić, J., Posa, M., Bjelica, A., Miladinović, J., Rizou, M., Aldawoud, T. M. S.,& Galanakis, C. M.. (2021). Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content. in Journal of Food Composition & Analysis
Academic Press Inc Elsevier Science, San Diego., 95.
https://doi.org/10.1016/j.jfca.2020.103670

Tepavčević V, Cvejić J, Posa M, Bjelica A, Miladinović J, Rizou M, Aldawoud TMS, Galanakis CM. Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content. in Journal of Food Composition & Analysis. 2021;95.
doi:10.1016/j.jfca.2020.103670 .

Tepavčević, Vesna, Cvejić, Jelena, Posa, Mihalj, Bjelica, Artur, Miladinović, Jegor, Rizou, Myrto, Aldawoud, Turki M. S., Galanakis, Charis M., "Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content" in Journal of Food Composition & Analysis, 95 (2021),
https://doi.org/10.1016/j.jfca.2020.103670 . .

TY  - JOUR
AU  - Miladinović, Jegor
AU  - Đorđević, Vuk
AU  - Balešević-Tubić, Svetlana
AU  - Petrović, Kristina
AU  - Ćeran, Marina
AU  - Cvejić, Jelena
AU  - Bursać, Mira
AU  - Miladinović, Dragana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1891
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Author Correction: Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material (vol 9, 10341, 2019)
VL  - 9
DO  - 10.1038/s41598-019-53778-y
ER  - 

@article{
author = "Miladinović, Jegor and Đorđević, Vuk and Balešević-Tubić, Svetlana and Petrović, Kristina and Ćeran, Marina and Cvejić, Jelena and Bursać, Mira and Miladinović, Dragana",
year = "2019",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Author Correction: Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material (vol 9, 10341, 2019)",
volume = "9",
doi = "10.1038/s41598-019-53778-y"
}

Miladinović, J., Đorđević, V., Balešević-Tubić, S., Petrović, K., Ćeran, M., Cvejić, J., Bursać, M.,& Miladinović, D.. (2019). Author Correction: Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material (vol 9, 10341, 2019). in Scientific Reports
Nature Publishing Group, London., 9.
https://doi.org/10.1038/s41598-019-53778-y

Miladinović J, Đorđević V, Balešević-Tubić S, Petrović K, Ćeran M, Cvejić J, Bursać M, Miladinović D. Author Correction: Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material (vol 9, 10341, 2019). in Scientific Reports. 2019;9.
doi:10.1038/s41598-019-53778-y .

Miladinović, Jegor, Đorđević, Vuk, Balešević-Tubić, Svetlana, Petrović, Kristina, Ćeran, Marina, Cvejić, Jelena, Bursać, Mira, Miladinović, Dragana, "Author Correction: Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material (vol 9, 10341, 2019)" in Scientific Reports, 9 (2019),
https://doi.org/10.1038/s41598-019-53778-y . .

TY  - JOUR
AU  - Miladinović, Jegor
AU  - Đorđević, Vuk
AU  - Balešević-Tubić, Svetlana
AU  - Petrović, Kristina
AU  - Ćeran, Marina
AU  - Cvejić, Jelena
AU  - Bursać, Mira
AU  - Miladinović, Dragana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1854
AB  - Isoflavones are a group of phytoestrogens, naturally-occurring substances important for their role in human health. Legumes, particularly soybeans (Glycine max (L.) Merr.), are the richest source of isoflavones in human diet. Since there is not much current data on genetics of isoflavones in soybean, particularly in the aglycone form, elucidation of the mode of inheritance is necessary in order to design an efficient breeding strategy for the development of high-isoflavone soybean genotypes. Based on the isoflavone content in 23 samples of soybeans from four different maturity groups (00, 0, I and II), three crosses were made in order to determine the inheritance pattern and increase the content of total isoflavones and their aglycone form. Genotype with the lowest total isoflavone content (NS-L-146) was crossed with the low-(NS Zenit), medium (NS Maximus), and high-(NS Virtus) isoflavone genotypes. There were no significant differences in the total isoflavone content (TIF) between F-2 populations, and there was no transgression among genotypes within the populations. Each genotype within all three populations had a higher TIF value than the lower parent (NS-L-146), while genotypes with a higher TIF value than the better parent were found only in the NS-L-146 x NS Zenit cross. However, significant differences in the aglycone ratio (ratio of aglycone to glycone form of isoflavones) were found between the populations. The highest aglycone ratio was found in the NS-L-146 x NS Maximus cross. The results indicate that the genetic improvement for the trait is possible.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material
VL  - 9
DO  - 10.1038/s41598-019-46817-1
ER  - 

@article{
author = "Miladinović, Jegor and Đorđević, Vuk and Balešević-Tubić, Svetlana and Petrović, Kristina and Ćeran, Marina and Cvejić, Jelena and Bursać, Mira and Miladinović, Dragana",
year = "2019",
abstract = "Isoflavones are a group of phytoestrogens, naturally-occurring substances important for their role in human health. Legumes, particularly soybeans (Glycine max (L.) Merr.), are the richest source of isoflavones in human diet. Since there is not much current data on genetics of isoflavones in soybean, particularly in the aglycone form, elucidation of the mode of inheritance is necessary in order to design an efficient breeding strategy for the development of high-isoflavone soybean genotypes. Based on the isoflavone content in 23 samples of soybeans from four different maturity groups (00, 0, I and II), three crosses were made in order to determine the inheritance pattern and increase the content of total isoflavones and their aglycone form. Genotype with the lowest total isoflavone content (NS-L-146) was crossed with the low-(NS Zenit), medium (NS Maximus), and high-(NS Virtus) isoflavone genotypes. There were no significant differences in the total isoflavone content (TIF) between F-2 populations, and there was no transgression among genotypes within the populations. Each genotype within all three populations had a higher TIF value than the lower parent (NS-L-146), while genotypes with a higher TIF value than the better parent were found only in the NS-L-146 x NS Zenit cross. However, significant differences in the aglycone ratio (ratio of aglycone to glycone form of isoflavones) were found between the populations. The highest aglycone ratio was found in the NS-L-146 x NS Maximus cross. The results indicate that the genetic improvement for the trait is possible.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material",
volume = "9",
doi = "10.1038/s41598-019-46817-1"
}

Miladinović, J., Đorđević, V., Balešević-Tubić, S., Petrović, K., Ćeran, M., Cvejić, J., Bursać, M.,& Miladinović, D.. (2019). Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material. in Scientific Reports
Nature Publishing Group, London., 9.
https://doi.org/10.1038/s41598-019-46817-1

Miladinović J, Đorđević V, Balešević-Tubić S, Petrović K, Ćeran M, Cvejić J, Bursać M, Miladinović D. Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material. in Scientific Reports. 2019;9.
doi:10.1038/s41598-019-46817-1 .

Miladinović, Jegor, Đorđević, Vuk, Balešević-Tubić, Svetlana, Petrović, Kristina, Ćeran, Marina, Cvejić, Jelena, Bursać, Mira, Miladinović, Dragana, "Increase of isoflavones in the aglycone form in soybeans by targeted crossings of cultivated breeding material" in Scientific Reports, 9 (2019),
https://doi.org/10.1038/s41598-019-46817-1 . .

TY  - JOUR
AU  - Bursać, Mira
AU  - Atanacković-Krstonosić, Milica
AU  - Miladinović, Jegor
AU  - Malenčić, Ðorđe
AU  - Gvozdenović, Ljiljana
AU  - Cvejić, Jelena
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1715
AB  - The influence of soybean seed coat color and genotype on antioxidant capacity, phenolic content and isoflavone profile was investigated. Isoflavone content and composition of 21 seed samples - yellow, green, brown, black and rarely mentioned ocher and reddish, were determined by high-performance liquid chromatographic analysis. Antioxidant capacity and total phenolic content (TPC) were evaluated spectrophotometrically. Significant correlation between total isoflavone content and total genisteins was established in all colored groups. Total isoflavone content was in the range from 2.11 mg/g in a green wrinkled sample to 5.24 mg/g in yellow seed. It was found that black genotypes had the highest TPC and antioxidant capacity, which were significantly different (p lt 0.05) from other colored soybeans. The obtained interconnections among analyzed isoflavones can be used as a model for estimation of their specific content.
PB  - Natural Product Incorporation
T2  - Natural Product Communications
T1  - Isoflavone Composition, Total Phenolic Content and Antioxidant Capacity of Soybeans with Colored Seed Coat
EP  - 532
IS  - 4
SP  - 527
VL  - 12
DO  - 10.1177/1934578x1701200417
ER  - 

@article{
author = "Bursać, Mira and Atanacković-Krstonosić, Milica and Miladinović, Jegor and Malenčić, Ðorđe and Gvozdenović, Ljiljana and Cvejić, Jelena",
year = "2017",
abstract = "The influence of soybean seed coat color and genotype on antioxidant capacity, phenolic content and isoflavone profile was investigated. Isoflavone content and composition of 21 seed samples - yellow, green, brown, black and rarely mentioned ocher and reddish, were determined by high-performance liquid chromatographic analysis. Antioxidant capacity and total phenolic content (TPC) were evaluated spectrophotometrically. Significant correlation between total isoflavone content and total genisteins was established in all colored groups. Total isoflavone content was in the range from 2.11 mg/g in a green wrinkled sample to 5.24 mg/g in yellow seed. It was found that black genotypes had the highest TPC and antioxidant capacity, which were significantly different (p lt 0.05) from other colored soybeans. The obtained interconnections among analyzed isoflavones can be used as a model for estimation of their specific content.",
publisher = "Natural Product Incorporation",
journal = "Natural Product Communications",
title = "Isoflavone Composition, Total Phenolic Content and Antioxidant Capacity of Soybeans with Colored Seed Coat",
pages = "532-527",
number = "4",
volume = "12",
doi = "10.1177/1934578x1701200417"
}

Bursać, M., Atanacković-Krstonosić, M., Miladinović, J., Malenčić, Ð., Gvozdenović, L.,& Cvejić, J.. (2017). Isoflavone Composition, Total Phenolic Content and Antioxidant Capacity of Soybeans with Colored Seed Coat. in Natural Product Communications
Natural Product Incorporation., 12(4), 527-532.
https://doi.org/10.1177/1934578x1701200417

Bursać M, Atanacković-Krstonosić M, Miladinović J, Malenčić Ð, Gvozdenović L, Cvejić J. Isoflavone Composition, Total Phenolic Content and Antioxidant Capacity of Soybeans with Colored Seed Coat. in Natural Product Communications. 2017;12(4):527-532.
doi:10.1177/1934578x1701200417 .

Bursać, Mira, Atanacković-Krstonosić, Milica, Miladinović, Jegor, Malenčić, Ðorđe, Gvozdenović, Ljiljana, Cvejić, Jelena, "Isoflavone Composition, Total Phenolic Content and Antioxidant Capacity of Soybeans with Colored Seed Coat" in Natural Product Communications, 12, no. 4 (2017):527-532,
https://doi.org/10.1177/1934578x1701200417 . .

TY  - JOUR
AU  - Kiprovski, Biljana
AU  - Malenčić, Ðorđe
AU  - Đurić, Simonida
AU  - Bursać, Mira
AU  - Cvejić, Jelena
AU  - Sikora, Vladimir
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1576
AB  - Plant-growth promoting rhizobacteria (PGPR) elicit activation of the phenylpropanoid pathway in plants, which leads to phenolics production and enhanced antioxidant capacity. The purpose of this work was to assess the antioxidant activity of soybean plants, Glycine max L., inoculated with PGPR (isolates of Azotobacter chroococcum, Streptomyces sp. and mixture of these) during plant development, as well as the yield of inoculated soybean plants. PGPR applied in the experiment stimulated flavonoids and isoflavone synthesis, which enhanced the non-enzymatic antioxidant ability of the soybean plants. Moreover, PGPRs stimulated the accumulation of daidzein and genistin in soybean seedlings (5-fold and 2-fold compared to the control values, respectively). The mixture of PGPRs had a positive impact on the antioxidant activity (10-20 % higher activity) and yield components of soybean, which proposed this inoculum as possibly a potent bio-fertilizer in soybean production.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of The Serbian Chemical Society
T1  - Isoflavone content and antioxidant activity of soybean inoculated with plant-growth promoting rhizobacteria
EP  - 1249
IS  - 11
SP  - 1239
VL  - 81
DO  - 10.2298/JSC160422070K
ER  - 

@article{
author = "Kiprovski, Biljana and Malenčić, Ðorđe and Đurić, Simonida and Bursać, Mira and Cvejić, Jelena and Sikora, Vladimir",
year = "2016",
abstract = "Plant-growth promoting rhizobacteria (PGPR) elicit activation of the phenylpropanoid pathway in plants, which leads to phenolics production and enhanced antioxidant capacity. The purpose of this work was to assess the antioxidant activity of soybean plants, Glycine max L., inoculated with PGPR (isolates of Azotobacter chroococcum, Streptomyces sp. and mixture of these) during plant development, as well as the yield of inoculated soybean plants. PGPR applied in the experiment stimulated flavonoids and isoflavone synthesis, which enhanced the non-enzymatic antioxidant ability of the soybean plants. Moreover, PGPRs stimulated the accumulation of daidzein and genistin in soybean seedlings (5-fold and 2-fold compared to the control values, respectively). The mixture of PGPRs had a positive impact on the antioxidant activity (10-20 % higher activity) and yield components of soybean, which proposed this inoculum as possibly a potent bio-fertilizer in soybean production.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of The Serbian Chemical Society",
title = "Isoflavone content and antioxidant activity of soybean inoculated with plant-growth promoting rhizobacteria",
pages = "1249-1239",
number = "11",
volume = "81",
doi = "10.2298/JSC160422070K"
}

Kiprovski, B., Malenčić, Ð., Đurić, S., Bursać, M., Cvejić, J.,& Sikora, V.. (2016). Isoflavone content and antioxidant activity of soybean inoculated with plant-growth promoting rhizobacteria. in Journal of The Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 81(11), 1239-1249.
https://doi.org/10.2298/JSC160422070K

Kiprovski B, Malenčić Ð, Đurić S, Bursać M, Cvejić J, Sikora V. Isoflavone content and antioxidant activity of soybean inoculated with plant-growth promoting rhizobacteria. in Journal of The Serbian Chemical Society. 2016;81(11):1239-1249.
doi:10.2298/JSC160422070K .

Kiprovski, Biljana, Malenčić, Ðorđe, Đurić, Simonida, Bursać, Mira, Cvejić, Jelena, Sikora, Vladimir, "Isoflavone content and antioxidant activity of soybean inoculated with plant-growth promoting rhizobacteria" in Journal of The Serbian Chemical Society, 81, no. 11 (2016):1239-1249,
https://doi.org/10.2298/JSC160422070K . .

TY  - CONF
AU  - Bursać, Mira
AU  - Cvejić, Jelena
AU  - Vasiljević, Sanja
AU  - Krstić, Đorđe
AU  - Ćupina, Branko
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3041
AB  - Red clover (Trifolium pratense) is rich in phytoestrogens as well as other phenolic substances which contribute to its antioxidative properties. Still, its antioxidative potential has not been thoroughly investigated. The aim of this study was to determine radical scavenging capacity and total phenolic content of red clover cultivars from Serbia observing different plant parts. Leaves, stems and flowers from four red clover cultivars (Kolubara, Una, Avala, K17) were grounded and extraction with water, 3M HCl and ethanol was performed. Radical-scavenging capacity was measured by reaction with the stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and calculation of the amount necessary to decrease initial DPPH concentration by 50% (IC50). Total phenolic content (TPC) was determined according to the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). Antioxidant potential and TPC of leaves and flowers were not statistically different, but on average, flower extracts had the highest antioxidant activity (IC500.087 mg/ml) and leaf extracts the highest TPC (30.30 mg/g GAE).
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Antioxidant potential and total phenolic content of Serbian red clover cultivars
EP  - 292
SP  - 292
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3041
ER  - 

@conference{
author = "Bursać, Mira and Cvejić, Jelena and Vasiljević, Sanja and Krstić, Đorđe and Ćupina, Branko",
year = "2013",
abstract = "Red clover (Trifolium pratense) is rich in phytoestrogens as well as other phenolic substances which contribute to its antioxidative properties. Still, its antioxidative potential has not been thoroughly investigated. The aim of this study was to determine radical scavenging capacity and total phenolic content of red clover cultivars from Serbia observing different plant parts. Leaves, stems and flowers from four red clover cultivars (Kolubara, Una, Avala, K17) were grounded and extraction with water, 3M HCl and ethanol was performed. Radical-scavenging capacity was measured by reaction with the stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and calculation of the amount necessary to decrease initial DPPH concentration by 50% (IC50). Total phenolic content (TPC) was determined according to the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). Antioxidant potential and TPC of leaves and flowers were not statistically different, but on average, flower extracts had the highest antioxidant activity (IC500.087 mg/ml) and leaf extracts the highest TPC (30.30 mg/g GAE).",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Antioxidant potential and total phenolic content of Serbian red clover cultivars",
pages = "292-292",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3041"
}

Bursać, M., Cvejić, J., Vasiljević, S., Krstić, Đ.,& Ćupina, B.. (2013). Antioxidant potential and total phenolic content of Serbian red clover cultivars. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 292-292.
https://hdl.handle.net/21.15107/rcub_fiver_3041

Bursać M, Cvejić J, Vasiljević S, Krstić Đ, Ćupina B. Antioxidant potential and total phenolic content of Serbian red clover cultivars. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:292-292.
https://hdl.handle.net/21.15107/rcub_fiver_3041 .

Bursać, Mira, Cvejić, Jelena, Vasiljević, Sanja, Krstić, Đorđe, Ćupina, Branko, "Antioxidant potential and total phenolic content of Serbian red clover cultivars" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):292-292,
https://hdl.handle.net/21.15107/rcub_fiver_3041 .

TY  - JOUR
AU  - Malenčić, Ðorđe
AU  - Cvejić, Jelena
AU  - Tepavčević, Vesna
AU  - Bursać, Mira
AU  - Kiprovski, Biljana
AU  - Rajković, Miloš
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1216
AB  - Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.
PB  - Sciendo, Warsaw
T2  - Central European Journal of Biology
T1  - Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum
EP  - 929
IS  - 9
SP  - 921
VL  - 8
DO  - 10.2478/s11535-013-0201-1
ER  - 

@article{
author = "Malenčić, Ðorđe and Cvejić, Jelena and Tepavčević, Vesna and Bursać, Mira and Kiprovski, Biljana and Rajković, Miloš",
year = "2013",
abstract = "Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.",
publisher = "Sciendo, Warsaw",
journal = "Central European Journal of Biology",
title = "Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum",
pages = "929-921",
number = "9",
volume = "8",
doi = "10.2478/s11535-013-0201-1"
}

Malenčić, Ð., Cvejić, J., Tepavčević, V., Bursać, M., Kiprovski, B.,& Rajković, M.. (2013). Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum. in Central European Journal of Biology
Sciendo, Warsaw., 8(9), 921-929.
https://doi.org/10.2478/s11535-013-0201-1

Malenčić Ð, Cvejić J, Tepavčević V, Bursać M, Kiprovski B, Rajković M. Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum. in Central European Journal of Biology. 2013;8(9):921-929.
doi:10.2478/s11535-013-0201-1 .

Malenčić, Ðorđe, Cvejić, Jelena, Tepavčević, Vesna, Bursać, Mira, Kiprovski, Biljana, Rajković, Miloš, "Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum" in Central European Journal of Biology, 8, no. 9 (2013):921-929,
https://doi.org/10.2478/s11535-013-0201-1 . .

TY  - JOUR
AU  - Malenčić, Ðorđe
AU  - Cvejić, Jelena
AU  - Miladinović, Jegor
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1074
AB  - The antioxidant activity and contents of various polyphenol classes in the seeds of seven soybean varieties of different seed color and one yellow seed cultivar, representing a reference genotype, were evaluated. Total polyphenols and tannins were determined after extraction of plant material with 70% aqueous acetone, and total flavonoids were extracted with methanol and acetic acid, whereas anthocyanins were extracted with 20% aqueous ethanol. In addition, isoflavone content and composition were determined using high-performance liquid chromatography analysis. Antioxidant activity of seed extracts was evaluated by the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity assay. A positive linear correlation between antioxidant activity and contents of total polyphenols and anthocyanins was established. The highest antioxidant activity was observed in the extracts of black and brown varieties, which also showed high levels of all polyphenol classes examined. Yellow seed had the highest total isoflavone content (3.62 mg/g of dry material). The highest concentration of total daidzein was determined in black seeds (>2.0 mg/g of dry material), and the highest total glycitein and genistein contents occurred in the yellow cultivar (0.53 and 1.49 mg/g of dry material, respectively). According to our results, varieties of black and brown seeds could be of special interest not only for their large content of total polyphenols, ranging from 4.94 to 6.22mg of gallic acid equivalents/g of dry material, but also for their high content of natural antioxidants such as anthocyanins.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Polyphenol Content and Antioxidant Properties of Colored Soybean Seeds from Central Europe
EP  - 95
IS  - 1
SP  - 89
VL  - 15
DO  - 10.1089/jmf.2010.0329
ER  - 

@article{
author = "Malenčić, Ðorđe and Cvejić, Jelena and Miladinović, Jegor",
year = "2012",
abstract = "The antioxidant activity and contents of various polyphenol classes in the seeds of seven soybean varieties of different seed color and one yellow seed cultivar, representing a reference genotype, were evaluated. Total polyphenols and tannins were determined after extraction of plant material with 70% aqueous acetone, and total flavonoids were extracted with methanol and acetic acid, whereas anthocyanins were extracted with 20% aqueous ethanol. In addition, isoflavone content and composition were determined using high-performance liquid chromatography analysis. Antioxidant activity of seed extracts was evaluated by the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity assay. A positive linear correlation between antioxidant activity and contents of total polyphenols and anthocyanins was established. The highest antioxidant activity was observed in the extracts of black and brown varieties, which also showed high levels of all polyphenol classes examined. Yellow seed had the highest total isoflavone content (3.62 mg/g of dry material). The highest concentration of total daidzein was determined in black seeds (>2.0 mg/g of dry material), and the highest total glycitein and genistein contents occurred in the yellow cultivar (0.53 and 1.49 mg/g of dry material, respectively). According to our results, varieties of black and brown seeds could be of special interest not only for their large content of total polyphenols, ranging from 4.94 to 6.22mg of gallic acid equivalents/g of dry material, but also for their high content of natural antioxidants such as anthocyanins.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Polyphenol Content and Antioxidant Properties of Colored Soybean Seeds from Central Europe",
pages = "95-89",
number = "1",
volume = "15",
doi = "10.1089/jmf.2010.0329"
}

Malenčić, Ð., Cvejić, J.,& Miladinović, J.. (2012). Polyphenol Content and Antioxidant Properties of Colored Soybean Seeds from Central Europe. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 15(1), 89-95.
https://doi.org/10.1089/jmf.2010.0329

Malenčić Ð, Cvejić J, Miladinović J. Polyphenol Content and Antioxidant Properties of Colored Soybean Seeds from Central Europe. in Journal of Medicinal Food. 2012;15(1):89-95.
doi:10.1089/jmf.2010.0329 .

Malenčić, Ðorđe, Cvejić, Jelena, Miladinović, Jegor, "Polyphenol Content and Antioxidant Properties of Colored Soybean Seeds from Central Europe" in Journal of Medicinal Food, 15, no. 1 (2012):89-95,
https://doi.org/10.1089/jmf.2010.0329 . .

TY  - CONF
AU  - Bursać, Mira
AU  - Atanacković, Milica
AU  - Cvejić, Jelena
AU  - Vasiljević, Sanja
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/1024
PB  - Georg Thieme Verlag Kg, Stuttgart
C3  - Planta Medica
T1  - Isoflavone profiles in different plant parts of red clover
EP  - 1365
IS  - 12
SP  - 1365
VL  - 77
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1024
ER  - 

@conference{
author = "Bursać, Mira and Atanacković, Milica and Cvejić, Jelena and Vasiljević, Sanja",
year = "2011",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "Isoflavone profiles in different plant parts of red clover",
pages = "1365-1365",
number = "12",
volume = "77",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1024"
}

Bursać, M., Atanacković, M., Cvejić, J.,& Vasiljević, S.. (2011). Isoflavone profiles in different plant parts of red clover. in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 77(12), 1365-1365.
https://hdl.handle.net/21.15107/rcub_fiver_1024

Bursać M, Atanacković M, Cvejić J, Vasiljević S. Isoflavone profiles in different plant parts of red clover. in Planta Medica. 2011;77(12):1365-1365.
https://hdl.handle.net/21.15107/rcub_fiver_1024 .

Bursać, Mira, Atanacković, Milica, Cvejić, Jelena, Vasiljević, Sanja, "Isoflavone profiles in different plant parts of red clover" in Planta Medica, 77, no. 12 (2011):1365-1365,
https://hdl.handle.net/21.15107/rcub_fiver_1024 .

TY  - JOUR
AU  - Bursać, Mira
AU  - Atanacković, Milica
AU  - Cvejić, Jelena
AU  - Vasiljević, Sanja
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/991
AB  - Introduction: Red clover (Trifolium pratense L., Fabaceae) is one of the main sources of phytoestrogens, among which are the most present isoflavones: daidzein, genistein, formononetin and biochanin A. These substances are used for reduction of menopausal symptoms, and they are considered to have certain role in prevention of osteoporosis, cancer and cardiovascular diseases. Aim: The aim of this study was to determine content of isoflavones in different red clover cultivars, cultivated in Vojvodina, and also in their plant parts. Material and methods: Five cultivars were analyzed (stem, leaf and flower). After extraction, isoflavones were identified and quantified in samples by high-performance liquid chromatography (HPLC). Results: Total isoflavone content varied between 0,41 do 3,81 mg/g, and it was on average the highest in leaves (2,73±0,97 mg/g), and the lowest in stems of red clover cultivars (0,47±0,06 mg/g). In leaves formononetin was dominant (on average 1,62±0,97 mg/g), while the lowest average content had biochanin A in stems (0,04±0,06 mg/g). Cultivar m21 had the highest average total isoflavone content in stem, leaf and flower - 1,78±1,77 mg/g. Conclusion: The highest concentration of isoflavones was determined in leaves. Cultivar m21 was the richest in isoflavones, and on average in all analyzed samples formononetin was the most present isoflavone. .
AB  - Uvod: Crvena detelina (Trifolium pratense L., Fabaceae) je jedan od značajnijih izvora fitoestrogena, od kojih su najzastupljeniji izoflavoni: daidzein, genistein, formononetin i biohanin A. Ove supstance se koriste za ublažavanje tegoba u menopauzi, a smatra se da mogu da imaju ulogu u prevenciji osteoporoze, kancera i kardioloških poremećaja. Cilj: Cilj rad je utvrđivanje sadržaja pojedinačnih izoflavona različitih sorti crvene deteline gajenih na području Vojvodine, kao i u pojedinim delovima biljke. Materijal i metode: Analizirano je pet sorti crvene deteline (stabljika, list i cvet). Nakon ekstrakcije, u uzorcima su identifikovani i kvantifikovani izoflavoni visoko-efikasnom tečnom hromatogafijom (HPLC). Rezultati: Sadržaj ukupnih izoflavona varira od 0,41 do 3,81 mg/g, a u proseku najzastupljeniji su u listovima (2,73±0,97 mg/g), dok stabljike crvene deteline sadrže najmanju koncentraciju tih jedinjenja (0,47±0,06 mg/g). U listovima je dominantan formononetin (prosečno 1,62±0,97 mg/g), dok je najmanji prosečni sadržaj biohanina A u stabljikama ispitivanih uzoraka (0,04±0,06 mg/g). U sorti m21 prosečna vrednost ukupnih izoflavona u stablu, listu i cvetu je najveća i iznosi 1,78±1,77 mg/g. Zaključak: Najveća koncentracija izoflavona utvrđena je u listovima. Sorta m21 je najbogatija izoflavonima, a u svim analiziranim uzorcima, u proseku je najviše zastupljen formononetin. .
PB  - Nestranačko, nevladino i neprofitno udruženje građana Medicina danas - pokret evropskih inicijativa, Novi Sad
T2  - Medicina danas
T1  - Analysis of phytoestrogens in red clover
T1  - Analiza fitoestrogena crvene deteline
EP  - 265
IS  - 7-9
SP  - 259
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_fiver_991
ER  - 

@article{
author = "Bursać, Mira and Atanacković, Milica and Cvejić, Jelena and Vasiljević, Sanja",
year = "2011",
abstract = "Introduction: Red clover (Trifolium pratense L., Fabaceae) is one of the main sources of phytoestrogens, among which are the most present isoflavones: daidzein, genistein, formononetin and biochanin A. These substances are used for reduction of menopausal symptoms, and they are considered to have certain role in prevention of osteoporosis, cancer and cardiovascular diseases. Aim: The aim of this study was to determine content of isoflavones in different red clover cultivars, cultivated in Vojvodina, and also in their plant parts. Material and methods: Five cultivars were analyzed (stem, leaf and flower). After extraction, isoflavones were identified and quantified in samples by high-performance liquid chromatography (HPLC). Results: Total isoflavone content varied between 0,41 do 3,81 mg/g, and it was on average the highest in leaves (2,73±0,97 mg/g), and the lowest in stems of red clover cultivars (0,47±0,06 mg/g). In leaves formononetin was dominant (on average 1,62±0,97 mg/g), while the lowest average content had biochanin A in stems (0,04±0,06 mg/g). Cultivar m21 had the highest average total isoflavone content in stem, leaf and flower - 1,78±1,77 mg/g. Conclusion: The highest concentration of isoflavones was determined in leaves. Cultivar m21 was the richest in isoflavones, and on average in all analyzed samples formononetin was the most present isoflavone. ., Uvod: Crvena detelina (Trifolium pratense L., Fabaceae) je jedan od značajnijih izvora fitoestrogena, od kojih su najzastupljeniji izoflavoni: daidzein, genistein, formononetin i biohanin A. Ove supstance se koriste za ublažavanje tegoba u menopauzi, a smatra se da mogu da imaju ulogu u prevenciji osteoporoze, kancera i kardioloških poremećaja. Cilj: Cilj rad je utvrđivanje sadržaja pojedinačnih izoflavona različitih sorti crvene deteline gajenih na području Vojvodine, kao i u pojedinim delovima biljke. Materijal i metode: Analizirano je pet sorti crvene deteline (stabljika, list i cvet). Nakon ekstrakcije, u uzorcima su identifikovani i kvantifikovani izoflavoni visoko-efikasnom tečnom hromatogafijom (HPLC). Rezultati: Sadržaj ukupnih izoflavona varira od 0,41 do 3,81 mg/g, a u proseku najzastupljeniji su u listovima (2,73±0,97 mg/g), dok stabljike crvene deteline sadrže najmanju koncentraciju tih jedinjenja (0,47±0,06 mg/g). U listovima je dominantan formononetin (prosečno 1,62±0,97 mg/g), dok je najmanji prosečni sadržaj biohanina A u stabljikama ispitivanih uzoraka (0,04±0,06 mg/g). U sorti m21 prosečna vrednost ukupnih izoflavona u stablu, listu i cvetu je najveća i iznosi 1,78±1,77 mg/g. Zaključak: Najveća koncentracija izoflavona utvrđena je u listovima. Sorta m21 je najbogatija izoflavonima, a u svim analiziranim uzorcima, u proseku je najviše zastupljen formononetin. .",
publisher = "Nestranačko, nevladino i neprofitno udruženje građana Medicina danas - pokret evropskih inicijativa, Novi Sad",
journal = "Medicina danas",
title = "Analysis of phytoestrogens in red clover, Analiza fitoestrogena crvene deteline",
pages = "265-259",
number = "7-9",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_fiver_991"
}

Bursać, M., Atanacković, M., Cvejić, J.,& Vasiljević, S.. (2011). Analysis of phytoestrogens in red clover. in Medicina danas
Nestranačko, nevladino i neprofitno udruženje građana Medicina danas - pokret evropskih inicijativa, Novi Sad., 10(7-9), 259-265.
https://hdl.handle.net/21.15107/rcub_fiver_991

Bursać M, Atanacković M, Cvejić J, Vasiljević S. Analysis of phytoestrogens in red clover. in Medicina danas. 2011;10(7-9):259-265.
https://hdl.handle.net/21.15107/rcub_fiver_991 .

Bursać, Mira, Atanacković, Milica, Cvejić, Jelena, Vasiljević, Sanja, "Analysis of phytoestrogens in red clover" in Medicina danas, 10, no. 7-9 (2011):259-265,
https://hdl.handle.net/21.15107/rcub_fiver_991 .

TY  - JOUR
AU  - Cvejić, Jelena
AU  - Tepavčević, Vesna
AU  - Bursać, Mira
AU  - Miladinović, Jegor
AU  - Malenčić, Ðorđe
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/1020
AB  - Twenty F1 soybean progenies were analyzed for isoflavone content in order to investigate whether this attribute can be translated from parents to the corresponding hybrids. Content of total isoflavones in analyzed samples was ranged from 1.56 to 3.66 mg/g of dried soybean. Ten analyzed F1 progenies in one group had a mutual parent while the rest in the second group had randomly chosen parents. Contents of total isoflavones. total daidzeins and total genisteins were significantly different (p lt 0.05) in these two sample groups. A significantly strong correlation (p lt 0.05) between total isoflavone content in parents and corresponding F1 progenies was established (R-2 = 0.915). Similarity of the F1 progenies with a mutual parent was confirmed by Principal Component Analysis. Obtained results suggest that isoflavone content is the trait that can be derived from parent genotype to its F1 progenies and therefore the breeding of genotypes with better health promoting characteristics can be directed.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Isoflavone composition in F1 soybean progenies
EP  - 2702
IS  - 9
SP  - 2698
VL  - 44
DO  - 10.1016/j.foodres.2011.05.024
ER  - 

@article{
author = "Cvejić, Jelena and Tepavčević, Vesna and Bursać, Mira and Miladinović, Jegor and Malenčić, Ðorđe",
year = "2011",
abstract = "Twenty F1 soybean progenies were analyzed for isoflavone content in order to investigate whether this attribute can be translated from parents to the corresponding hybrids. Content of total isoflavones in analyzed samples was ranged from 1.56 to 3.66 mg/g of dried soybean. Ten analyzed F1 progenies in one group had a mutual parent while the rest in the second group had randomly chosen parents. Contents of total isoflavones. total daidzeins and total genisteins were significantly different (p lt 0.05) in these two sample groups. A significantly strong correlation (p lt 0.05) between total isoflavone content in parents and corresponding F1 progenies was established (R-2 = 0.915). Similarity of the F1 progenies with a mutual parent was confirmed by Principal Component Analysis. Obtained results suggest that isoflavone content is the trait that can be derived from parent genotype to its F1 progenies and therefore the breeding of genotypes with better health promoting characteristics can be directed.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Isoflavone composition in F1 soybean progenies",
pages = "2702-2698",
number = "9",
volume = "44",
doi = "10.1016/j.foodres.2011.05.024"
}

Cvejić, J., Tepavčević, V., Bursać, M., Miladinović, J.,& Malenčić, Ð.. (2011). Isoflavone composition in F1 soybean progenies. in Food Research International
Elsevier, Amsterdam., 44(9), 2698-2702.
https://doi.org/10.1016/j.foodres.2011.05.024

Cvejić J, Tepavčević V, Bursać M, Miladinović J, Malenčić Ð. Isoflavone composition in F1 soybean progenies. in Food Research International. 2011;44(9):2698-2702.
doi:10.1016/j.foodres.2011.05.024 .

Cvejić, Jelena, Tepavčević, Vesna, Bursać, Mira, Miladinović, Jegor, Malenčić, Ðorđe, "Isoflavone composition in F1 soybean progenies" in Food Research International, 44, no. 9 (2011):2698-2702,
https://doi.org/10.1016/j.foodres.2011.05.024 . .

TY  - JOUR
AU  - Tepavčević, Vesna
AU  - Atanacković, Milica
AU  - Miladinović, Jegor
AU  - Malenčić, Ðorđe
AU  - Popović, Jovan
AU  - Cvejić, Jelena
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/818
AB  - Twenty soybean cultivars, originating from the United States, Russia, Serbia, and China, were analyzed for their isoflavone composition, total polyphenolic content, and antioxidant activity. Isoflavones were extracted by aqueous methanol (80%) and analyzed by high-performance liquid chromatography/diode array detection. Precision and linearity of the applied method were within the standard limits of validation. The highest and the lowest total isoflavone contents were 4.59 and 1.45 mg/g of dried soybean weight, respectively. A significant difference (P lt .05) was found in isoflavone concentration among the different cultivars, but it was observed that origin is not a significant factor that could influence isoflavone content in soybeans. Total polyphenolic content varied between 2.13 and 3.45 mg of gallic acid equivalents/g of dried soybean weight. The free radical scavenging activity of soybean extracts assayed by 2,2-diphenyl-1-picrylhydrazyl in terms of 50% inhibitory concentration (IC50) ranged from 1.40 to 3.35 mg/mL. Negative correlation between total polyphenolic content and IC50 was observed, but there was no correlation between total isoflavone content and IC50. On the basis of this study, soybean cultivars with larger potency for biological activity could be recognized.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin
EP  - 664
IS  - 3
SP  - 657
VL  - 13
DO  - 10.1089/jmf.2009.0050
ER  - 

@article{
author = "Tepavčević, Vesna and Atanacković, Milica and Miladinović, Jegor and Malenčić, Ðorđe and Popović, Jovan and Cvejić, Jelena",
year = "2010",
abstract = "Twenty soybean cultivars, originating from the United States, Russia, Serbia, and China, were analyzed for their isoflavone composition, total polyphenolic content, and antioxidant activity. Isoflavones were extracted by aqueous methanol (80%) and analyzed by high-performance liquid chromatography/diode array detection. Precision and linearity of the applied method were within the standard limits of validation. The highest and the lowest total isoflavone contents were 4.59 and 1.45 mg/g of dried soybean weight, respectively. A significant difference (P lt .05) was found in isoflavone concentration among the different cultivars, but it was observed that origin is not a significant factor that could influence isoflavone content in soybeans. Total polyphenolic content varied between 2.13 and 3.45 mg of gallic acid equivalents/g of dried soybean weight. The free radical scavenging activity of soybean extracts assayed by 2,2-diphenyl-1-picrylhydrazyl in terms of 50% inhibitory concentration (IC50) ranged from 1.40 to 3.35 mg/mL. Negative correlation between total polyphenolic content and IC50 was observed, but there was no correlation between total isoflavone content and IC50. On the basis of this study, soybean cultivars with larger potency for biological activity could be recognized.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin",
pages = "664-657",
number = "3",
volume = "13",
doi = "10.1089/jmf.2009.0050"
}

Tepavčević, V., Atanacković, M., Miladinović, J., Malenčić, Ð., Popović, J.,& Cvejić, J.. (2010). Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 13(3), 657-664.
https://doi.org/10.1089/jmf.2009.0050

Tepavčević V, Atanacković M, Miladinović J, Malenčić Ð, Popović J, Cvejić J. Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin. in Journal of Medicinal Food. 2010;13(3):657-664.
doi:10.1089/jmf.2009.0050 .

Tepavčević, Vesna, Atanacković, Milica, Miladinović, Jegor, Malenčić, Ðorđe, Popović, Jovan, Cvejić, Jelena, "Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin" in Journal of Medicinal Food, 13, no. 3 (2010):657-664,
https://doi.org/10.1089/jmf.2009.0050 . .

TY  - JOUR
AU  - Cvejić, Jelena
AU  - Malenčić, Ðorđe
AU  - Tepavčević, Vesna
AU  - Posa, Mihalj
AU  - Miladinović, Jegor
PY  - 2009
UR  - http://fiver.ifvcns.rs/handle/123456789/772
AB  - The growing clinical interest and use of soybean-based food products and extracts to increase dietary phytoestrogen intake have led to medical interest in the precise determination of the phytoestrogen composition of soybean products. We have analyzed the composition of these compounds in 20 domestic and introduced varieties of genetically non-modified soybean genotypes grown under the same agroecological conditions. The isoflavone content of soybeans cultivated in this region of Serbia has not been previously reported. The assays were performed, after extraction with methanol-water (8:2, v/v), by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detection. The total phytoestrogen concentration was found to be between 2.24 and 3.79 mg g(-1) dry bean weight. The total concentration of daidzein and its derivatives ranged from 0.96 to 1.82 mg g(-1), total glyciteines from 0.34-0.53 mg g(-1), and all genistein derivatives from 0.86-1.67 mg g(-1) dry bean weight. Given the high biological potency of phytoestrogens and their metabolic conversion products, our data suggest that phytoestrogen content screening could be a useful tool in the selection of soybean genotypes with improved health promoting characteristics.
PB  - Natural Product Incorporation
T2  - Natural Product Communications
T1  - Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia
EP  - 1074
IS  - 8
SP  - 1069
VL  - 4
DO  - 10.1177/1934578x0900400810
ER  - 

@article{
author = "Cvejić, Jelena and Malenčić, Ðorđe and Tepavčević, Vesna and Posa, Mihalj and Miladinović, Jegor",
year = "2009",
abstract = "The growing clinical interest and use of soybean-based food products and extracts to increase dietary phytoestrogen intake have led to medical interest in the precise determination of the phytoestrogen composition of soybean products. We have analyzed the composition of these compounds in 20 domestic and introduced varieties of genetically non-modified soybean genotypes grown under the same agroecological conditions. The isoflavone content of soybeans cultivated in this region of Serbia has not been previously reported. The assays were performed, after extraction with methanol-water (8:2, v/v), by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detection. The total phytoestrogen concentration was found to be between 2.24 and 3.79 mg g(-1) dry bean weight. The total concentration of daidzein and its derivatives ranged from 0.96 to 1.82 mg g(-1), total glyciteines from 0.34-0.53 mg g(-1), and all genistein derivatives from 0.86-1.67 mg g(-1) dry bean weight. Given the high biological potency of phytoestrogens and their metabolic conversion products, our data suggest that phytoestrogen content screening could be a useful tool in the selection of soybean genotypes with improved health promoting characteristics.",
publisher = "Natural Product Incorporation",
journal = "Natural Product Communications",
title = "Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia",
pages = "1074-1069",
number = "8",
volume = "4",
doi = "10.1177/1934578x0900400810"
}

Cvejić, J., Malenčić, Ð., Tepavčević, V., Posa, M.,& Miladinović, J.. (2009). Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia. in Natural Product Communications
Natural Product Incorporation., 4(8), 1069-1074.
https://doi.org/10.1177/1934578x0900400810

Cvejić J, Malenčić Ð, Tepavčević V, Posa M, Miladinović J. Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia. in Natural Product Communications. 2009;4(8):1069-1074.
doi:10.1177/1934578x0900400810 .

Cvejić, Jelena, Malenčić, Ðorđe, Tepavčević, Vesna, Posa, Mihalj, Miladinović, Jegor, "Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia" in Natural Product Communications, 4, no. 8 (2009):1069-1074,
https://doi.org/10.1177/1934578x0900400810 . .

TY  - JOUR
AU  - Tepavčević, Vesna
AU  - Cvejić, Jelena
AU  - Malenčić, Ðorđe
AU  - Miladinović, Jegor
AU  - Popović, Jovan
PY  - 2009
UR  - http://fiver.ifvcns.rs/handle/123456789/766
AB  - Twenty soybean cultivars were analysed for their antioxidant activity and content of isoflavones, flavonoids and total polyphenolics. After extraction with methanol-water (8:2, v/v), isoflavones were determined by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detector. Flavonoid and total polyphenolic content contents were established spectrophotometrically. Antioxidant activities of soybean extracts were measured using 2,2-diphenyl-1-pycril-hydrazil free radical method. Cultivars most abundant in polyphenolic compounds expressed the highest DPPH-radicals scavenging activities. Genotypes with low content of total polyphenolics, expressed approximately half of the activity of polyphenolic-rich genotypes. Correlation between total polyphenolic content and antioxidant activity was found to be statistically significant (p lt 0.01). At the same time, no correlation was found between content of isoflavones and antioxidant activity or content of flavonoids and antioxidant activity. The results suggested that total polyphenolic content could be considered a useful marker of antioxidant activity in soybeans and, beside isoflavone content, one of the criterions for identifying the cultivars with better health-promoting characteristics.
T2  - Oxidation Communications
T1  - Correlation of antioxidant activity with polyphenolic content in soybeans
EP  - 706
IS  - 3
SP  - 697
VL  - 32
UR  - https://hdl.handle.net/21.15107/rcub_fiver_766
ER  - 

@article{
author = "Tepavčević, Vesna and Cvejić, Jelena and Malenčić, Ðorđe and Miladinović, Jegor and Popović, Jovan",
year = "2009",
abstract = "Twenty soybean cultivars were analysed for their antioxidant activity and content of isoflavones, flavonoids and total polyphenolics. After extraction with methanol-water (8:2, v/v), isoflavones were determined by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detector. Flavonoid and total polyphenolic content contents were established spectrophotometrically. Antioxidant activities of soybean extracts were measured using 2,2-diphenyl-1-pycril-hydrazil free radical method. Cultivars most abundant in polyphenolic compounds expressed the highest DPPH-radicals scavenging activities. Genotypes with low content of total polyphenolics, expressed approximately half of the activity of polyphenolic-rich genotypes. Correlation between total polyphenolic content and antioxidant activity was found to be statistically significant (p lt 0.01). At the same time, no correlation was found between content of isoflavones and antioxidant activity or content of flavonoids and antioxidant activity. The results suggested that total polyphenolic content could be considered a useful marker of antioxidant activity in soybeans and, beside isoflavone content, one of the criterions for identifying the cultivars with better health-promoting characteristics.",
journal = "Oxidation Communications",
title = "Correlation of antioxidant activity with polyphenolic content in soybeans",
pages = "706-697",
number = "3",
volume = "32",
url = "https://hdl.handle.net/21.15107/rcub_fiver_766"
}

Tepavčević, V., Cvejić, J., Malenčić, Ð., Miladinović, J.,& Popović, J.. (2009). Correlation of antioxidant activity with polyphenolic content in soybeans. in Oxidation Communications, 32(3), 697-706.
https://hdl.handle.net/21.15107/rcub_fiver_766

Tepavčević V, Cvejić J, Malenčić Ð, Miladinović J, Popović J. Correlation of antioxidant activity with polyphenolic content in soybeans. in Oxidation Communications. 2009;32(3):697-706.
https://hdl.handle.net/21.15107/rcub_fiver_766 .

Tepavčević, Vesna, Cvejić, Jelena, Malenčić, Ðorđe, Miladinović, Jegor, Popović, Jovan, "Correlation of antioxidant activity with polyphenolic content in soybeans" in Oxidation Communications, 32, no. 3 (2009):697-706,
https://hdl.handle.net/21.15107/rcub_fiver_766 .

TY  - CONF
AU  - Aeran, V
AU  - Miladinović, Jegor
AU  - Cvejić, Jelena
PY  - 2007
UR  - http://fiver.ifvcns.rs/handle/123456789/489
PB  - Georg Thieme Verlag Kg, Stuttgart
C3  - Planta Medica
T1  - Isoflavone characterization in different genotypes of soybean
EP  - 910
IS  - 9
SP  - 909
VL  - 73
UR  - https://hdl.handle.net/21.15107/rcub_fiver_489
ER  - 

@conference{
author = "Aeran, V and Miladinović, Jegor and Cvejić, Jelena",
year = "2007",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "Isoflavone characterization in different genotypes of soybean",
pages = "910-909",
number = "9",
volume = "73",
url = "https://hdl.handle.net/21.15107/rcub_fiver_489"
}

Aeran, V., Miladinović, J.,& Cvejić, J.. (2007). Isoflavone characterization in different genotypes of soybean. in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 73(9), 909-910.
https://hdl.handle.net/21.15107/rcub_fiver_489

Aeran V, Miladinović J, Cvejić J. Isoflavone characterization in different genotypes of soybean. in Planta Medica. 2007;73(9):909-910.
https://hdl.handle.net/21.15107/rcub_fiver_489 .

Aeran, V, Miladinović, Jegor, Cvejić, Jelena, "Isoflavone characterization in different genotypes of soybean" in Planta Medica, 73, no. 9 (2007):909-910,
https://hdl.handle.net/21.15107/rcub_fiver_489 .