Antioxidant potential and total phenolic content of Serbian red clover cultivars
Апстракт
Red clover (Trifolium pratense) is rich in phytoestrogens as well as other phenolic substances which contribute to its antioxidative properties. Still, its antioxidative potential has not been thoroughly investigated. The aim of this study was to determine radical scavenging capacity and total phenolic content of red clover cultivars from Serbia observing different plant parts. Leaves, stems and flowers from four red clover cultivars (Kolubara, Una, Avala, K17) were grounded and extraction with water, 3M HCl and ethanol was performed. Radical-scavenging capacity was measured by reaction with the stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and calculation of the amount necessary to decrease initial DPPH concentration by 50% (IC50). Total phenolic content (TPC) was determined according to the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). Antioxidant potential and TPC of leaves and flowers were not statistically different, but on average, flower extracts h...ad the highest antioxidant activity (IC500.087 mg/ml) and leaf extracts the highest TPC (30.30 mg/g GAE).
Кључне речи:
red clover / cultivars / Serbia / phenolic content / antioxidant capacityИзвор:
Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad, 2013, 292-292Издавач:
- International Legume Society
- Novi Sad : Institute of Field and Vegetable Crops
Финансирање / пројекти:
- Унапређење технологије гајења крмних биљака на ораницама и травњацима (RS-MESTD-Technological Development (TD or TR)-31016)
Колекције
Институција/група
FiVeRTY - CONF AU - Bursać, Mira AU - Cvejić, Jelena AU - Vasiljević, Sanja AU - Krstić, Đorđe AU - Ćupina, Branko PY - 2013 UR - http://fiver.ifvcns.rs/handle/123456789/3041 AB - Red clover (Trifolium pratense) is rich in phytoestrogens as well as other phenolic substances which contribute to its antioxidative properties. Still, its antioxidative potential has not been thoroughly investigated. The aim of this study was to determine radical scavenging capacity and total phenolic content of red clover cultivars from Serbia observing different plant parts. Leaves, stems and flowers from four red clover cultivars (Kolubara, Una, Avala, K17) were grounded and extraction with water, 3M HCl and ethanol was performed. Radical-scavenging capacity was measured by reaction with the stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and calculation of the amount necessary to decrease initial DPPH concentration by 50% (IC50). Total phenolic content (TPC) was determined according to the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). Antioxidant potential and TPC of leaves and flowers were not statistically different, but on average, flower extracts had the highest antioxidant activity (IC500.087 mg/ml) and leaf extracts the highest TPC (30.30 mg/g GAE). PB - International Legume Society PB - Novi Sad : Institute of Field and Vegetable Crops C3 - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad T1 - Antioxidant potential and total phenolic content of Serbian red clover cultivars EP - 292 SP - 292 UR - https://hdl.handle.net/21.15107/rcub_fiver_3041 ER -
@conference{ author = "Bursać, Mira and Cvejić, Jelena and Vasiljević, Sanja and Krstić, Đorđe and Ćupina, Branko", year = "2013", abstract = "Red clover (Trifolium pratense) is rich in phytoestrogens as well as other phenolic substances which contribute to its antioxidative properties. Still, its antioxidative potential has not been thoroughly investigated. The aim of this study was to determine radical scavenging capacity and total phenolic content of red clover cultivars from Serbia observing different plant parts. Leaves, stems and flowers from four red clover cultivars (Kolubara, Una, Avala, K17) were grounded and extraction with water, 3M HCl and ethanol was performed. Radical-scavenging capacity was measured by reaction with the stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and calculation of the amount necessary to decrease initial DPPH concentration by 50% (IC50). Total phenolic content (TPC) was determined according to the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). Antioxidant potential and TPC of leaves and flowers were not statistically different, but on average, flower extracts had the highest antioxidant activity (IC500.087 mg/ml) and leaf extracts the highest TPC (30.30 mg/g GAE).", publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops", journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad", title = "Antioxidant potential and total phenolic content of Serbian red clover cultivars", pages = "292-292", url = "https://hdl.handle.net/21.15107/rcub_fiver_3041" }
Bursać, M., Cvejić, J., Vasiljević, S., Krstić, Đ.,& Ćupina, B.. (2013). Antioxidant potential and total phenolic content of Serbian red clover cultivars. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad International Legume Society., 292-292. https://hdl.handle.net/21.15107/rcub_fiver_3041
Bursać M, Cvejić J, Vasiljević S, Krstić Đ, Ćupina B. Antioxidant potential and total phenolic content of Serbian red clover cultivars. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:292-292. https://hdl.handle.net/21.15107/rcub_fiver_3041 .
Bursać, Mira, Cvejić, Jelena, Vasiljević, Sanja, Krstić, Đorđe, Ćupina, Branko, "Antioxidant potential and total phenolic content of Serbian red clover cultivars" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):292-292, https://hdl.handle.net/21.15107/rcub_fiver_3041 .