Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum
Само за регистроване кориснике
2013
Аутори
Malenčić, ÐorđeCvejić, Jelena
Tepavčević, Vesna
Bursać, Mira
Kiprovski, Biljana
Rajković, Miloš
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated culti...vars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.
Кључне речи:
Glycine max / Isoflavone phytoalexins / L-phenylalanine ammonia-lyase / Sclerotinia sclerotiorumИзвор:
Central European Journal of Biology, 2013, 8, 9, 921-929Издавач:
- Sciendo, Warsaw
Финансирање / пројекти:
- Интердисциплинарни приступ стварању нових сорти соје и унапређењу технологије гајења и дораде семена (RS-31022)
DOI: 10.2478/s11535-013-0201-1
ISSN: 1895-104X
WoS: 000321069400012
Scopus: 2-s2.0-84879733716
Колекције
Институција/група
FiVeRTY - JOUR AU - Malenčić, Ðorđe AU - Cvejić, Jelena AU - Tepavčević, Vesna AU - Bursać, Mira AU - Kiprovski, Biljana AU - Rajković, Miloš PY - 2013 UR - http://fiver.ifvcns.rs/handle/123456789/1216 AB - Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them. PB - Sciendo, Warsaw T2 - Central European Journal of Biology T1 - Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum EP - 929 IS - 9 SP - 921 VL - 8 DO - 10.2478/s11535-013-0201-1 ER -
@article{ author = "Malenčić, Ðorđe and Cvejić, Jelena and Tepavčević, Vesna and Bursać, Mira and Kiprovski, Biljana and Rajković, Miloš", year = "2013", abstract = "Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.", publisher = "Sciendo, Warsaw", journal = "Central European Journal of Biology", title = "Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum", pages = "929-921", number = "9", volume = "8", doi = "10.2478/s11535-013-0201-1" }
Malenčić, Ð., Cvejić, J., Tepavčević, V., Bursać, M., Kiprovski, B.,& Rajković, M.. (2013). Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum. in Central European Journal of Biology Sciendo, Warsaw., 8(9), 921-929. https://doi.org/10.2478/s11535-013-0201-1
Malenčić Ð, Cvejić J, Tepavčević V, Bursać M, Kiprovski B, Rajković M. Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum. in Central European Journal of Biology. 2013;8(9):921-929. doi:10.2478/s11535-013-0201-1 .
Malenčić, Ðorđe, Cvejić, Jelena, Tepavčević, Vesna, Bursać, Mira, Kiprovski, Biljana, Rajković, Miloš, "Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum" in Central European Journal of Biology, 8, no. 9 (2013):921-929, https://doi.org/10.2478/s11535-013-0201-1 . .