Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin
Само за регистроване кориснике
2010
Аутори
Tepavčević, VesnaAtanacković, Milica
Miladinović, Jegor
Malenčić, Ðorđe
Popović, Jovan
Cvejić, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Twenty soybean cultivars, originating from the United States, Russia, Serbia, and China, were analyzed for their isoflavone composition, total polyphenolic content, and antioxidant activity. Isoflavones were extracted by aqueous methanol (80%) and analyzed by high-performance liquid chromatography/diode array detection. Precision and linearity of the applied method were within the standard limits of validation. The highest and the lowest total isoflavone contents were 4.59 and 1.45 mg/g of dried soybean weight, respectively. A significant difference (P lt .05) was found in isoflavone concentration among the different cultivars, but it was observed that origin is not a significant factor that could influence isoflavone content in soybeans. Total polyphenolic content varied between 2.13 and 3.45 mg of gallic acid equivalents/g of dried soybean weight. The free radical scavenging activity of soybean extracts assayed by 2,2-diphenyl-1-picrylhydrazyl in terms of 50% inhibitory concentration... (IC50) ranged from 1.40 to 3.35 mg/mL. Negative correlation between total polyphenolic content and IC50 was observed, but there was no correlation between total isoflavone content and IC50. On the basis of this study, soybean cultivars with larger potency for biological activity could be recognized.
Кључне речи:
2,2-diphenyl-1-picrylhydrazyl assay / high-performance liquid chromatography analysis / soybean phytoestrogensИзвор:
Journal of Medicinal Food, 2010, 13, 3, 657-664Издавач:
- Mary Ann Liebert, Inc, New Rochelle
Финансирање / пројекти:
- Унапређење и развој оплемењивања и технологије производње соје (RS-MESTD-MPN2006-2010-20094)
DOI: 10.1089/jmf.2009.0050
ISSN: 1096-620X
PubMed: 20406140
WoS: 000278400600025
Scopus: 2-s2.0-77953745951
Колекције
Институција/група
FiVeRTY - JOUR AU - Tepavčević, Vesna AU - Atanacković, Milica AU - Miladinović, Jegor AU - Malenčić, Ðorđe AU - Popović, Jovan AU - Cvejić, Jelena PY - 2010 UR - http://fiver.ifvcns.rs/handle/123456789/818 AB - Twenty soybean cultivars, originating from the United States, Russia, Serbia, and China, were analyzed for their isoflavone composition, total polyphenolic content, and antioxidant activity. Isoflavones were extracted by aqueous methanol (80%) and analyzed by high-performance liquid chromatography/diode array detection. Precision and linearity of the applied method were within the standard limits of validation. The highest and the lowest total isoflavone contents were 4.59 and 1.45 mg/g of dried soybean weight, respectively. A significant difference (P lt .05) was found in isoflavone concentration among the different cultivars, but it was observed that origin is not a significant factor that could influence isoflavone content in soybeans. Total polyphenolic content varied between 2.13 and 3.45 mg of gallic acid equivalents/g of dried soybean weight. The free radical scavenging activity of soybean extracts assayed by 2,2-diphenyl-1-picrylhydrazyl in terms of 50% inhibitory concentration (IC50) ranged from 1.40 to 3.35 mg/mL. Negative correlation between total polyphenolic content and IC50 was observed, but there was no correlation between total isoflavone content and IC50. On the basis of this study, soybean cultivars with larger potency for biological activity could be recognized. PB - Mary Ann Liebert, Inc, New Rochelle T2 - Journal of Medicinal Food T1 - Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin EP - 664 IS - 3 SP - 657 VL - 13 DO - 10.1089/jmf.2009.0050 ER -
@article{ author = "Tepavčević, Vesna and Atanacković, Milica and Miladinović, Jegor and Malenčić, Ðorđe and Popović, Jovan and Cvejić, Jelena", year = "2010", abstract = "Twenty soybean cultivars, originating from the United States, Russia, Serbia, and China, were analyzed for their isoflavone composition, total polyphenolic content, and antioxidant activity. Isoflavones were extracted by aqueous methanol (80%) and analyzed by high-performance liquid chromatography/diode array detection. Precision and linearity of the applied method were within the standard limits of validation. The highest and the lowest total isoflavone contents were 4.59 and 1.45 mg/g of dried soybean weight, respectively. A significant difference (P lt .05) was found in isoflavone concentration among the different cultivars, but it was observed that origin is not a significant factor that could influence isoflavone content in soybeans. Total polyphenolic content varied between 2.13 and 3.45 mg of gallic acid equivalents/g of dried soybean weight. The free radical scavenging activity of soybean extracts assayed by 2,2-diphenyl-1-picrylhydrazyl in terms of 50% inhibitory concentration (IC50) ranged from 1.40 to 3.35 mg/mL. Negative correlation between total polyphenolic content and IC50 was observed, but there was no correlation between total isoflavone content and IC50. On the basis of this study, soybean cultivars with larger potency for biological activity could be recognized.", publisher = "Mary Ann Liebert, Inc, New Rochelle", journal = "Journal of Medicinal Food", title = "Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin", pages = "664-657", number = "3", volume = "13", doi = "10.1089/jmf.2009.0050" }
Tepavčević, V., Atanacković, M., Miladinović, J., Malenčić, Ð., Popović, J.,& Cvejić, J.. (2010). Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin. in Journal of Medicinal Food Mary Ann Liebert, Inc, New Rochelle., 13(3), 657-664. https://doi.org/10.1089/jmf.2009.0050
Tepavčević V, Atanacković M, Miladinović J, Malenčić Ð, Popović J, Cvejić J. Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin. in Journal of Medicinal Food. 2010;13(3):657-664. doi:10.1089/jmf.2009.0050 .
Tepavčević, Vesna, Atanacković, Milica, Miladinović, Jegor, Malenčić, Ðorđe, Popović, Jovan, Cvejić, Jelena, "Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin" in Journal of Medicinal Food, 13, no. 3 (2010):657-664, https://doi.org/10.1089/jmf.2009.0050 . .