TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2044
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Turnip Yellows Virus on Cabbage in Serbia
EP  - 998
IS  - 3
SP  - 997
VL  - 104
DO  - 10.1094/PDIS-08-19-1682-PDN
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Stanković, Ivana and Krstić, Branka",
year = "2020",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Turnip Yellows Virus on Cabbage in Serbia",
pages = "998-997",
number = "3",
volume = "104",
doi = "10.1094/PDIS-08-19-1682-PDN"
}

Milošević, D., Ignjatov, M., Vlajić, S., Nikolić, Z., Gvozdanović-Varga, J., Stanković, I.,& Krstić, B.. (2020). First Report of Turnip Yellows Virus on Cabbage in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 104(3), 997-998.
https://doi.org/10.1094/PDIS-08-19-1682-PDN

Milošević D, Ignjatov M, Vlajić S, Nikolić Z, Gvozdanović-Varga J, Stanković I, Krstić B. First Report of Turnip Yellows Virus on Cabbage in Serbia. in Plant Disease. 2020;104(3):997-998.
doi:10.1094/PDIS-08-19-1682-PDN .

Milošević, Dragana, Ignjatov, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Stanković, Ivana, Krstić, Branka, "First Report of Turnip Yellows Virus on Cabbage in Serbia" in Plant Disease, 104, no. 3 (2020):997-998,
https://doi.org/10.1094/PDIS-08-19-1682-PDN . .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Lazić, Dejan
AU  - Mitrović, Petar
AU  - Tanović, Brankica
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1904
AB  - Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples.
AB  - Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet
T1  - Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji
EP  - 29
IS  - 1
SP  - 19
VL  - 34
DO  - 10.2298/PIF1901019V
ER  - 

@article{
author = "Vojvodić, Mira and Lazić, Dejan and Mitrović, Petar and Tanović, Brankica and Vico, Ivana and Bulajić, Aleksandra",
year = "2019",
abstract = "Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples., Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet, Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji",
pages = "29-19",
number = "1",
volume = "34",
doi = "10.2298/PIF1901019V"
}

Vojvodić, M., Lazić, D., Mitrović, P., Tanović, B., Vico, I.,& Bulajić, A.. (2019). Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(1), 19-29.
https://doi.org/10.2298/PIF1901019V

Vojvodić M, Lazić D, Mitrović P, Tanović B, Vico I, Bulajić A. Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina. 2019;34(1):19-29.
doi:10.2298/PIF1901019V .

Vojvodić, Mira, Lazić, Dejan, Mitrović, Petar, Tanović, Brankica, Vico, Ivana, Bulajić, Aleksandra, "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet" in Pesticidi i fitomedicina, 34, no. 1 (2019):19-29,
https://doi.org/10.2298/PIF1901019V . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Krstić, Branka
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1896
AB  - In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.
AB  - Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia
T1  - Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji
EP  - 87
IS  - 3
SP  - 82
VL  - 56
DO  - 10.5937/ratpov56-23514
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Stanković, Ivana and Nikolić, Zorica and Tamindžić, Gordana and Krstić, Branka",
year = "2019",
abstract = "In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank., Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia, Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji",
pages = "87-82",
number = "3",
volume = "56",
doi = "10.5937/ratpov56-23514"
}

Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Stanković, I., Nikolić, Z., Tamindžić, G.,& Krstić, B.. (2019). Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 56(3), 82-87.
https://doi.org/10.5937/ratpov56-23514

Milošević D, Ignjatov M, Marjanović-Jeromela A, Stanković I, Nikolić Z, Tamindžić G, Krstić B. Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2019;56(3):82-87.
doi:10.5937/ratpov56-23514 .

Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Stanković, Ivana, Nikolić, Zorica, Tamindžić, Gordana, Krstić, Branka, "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 56, no. 3 (2019):82-87,
https://doi.org/10.5937/ratpov56-23514 . .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mihajlović, Milica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1759
AB  - Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet.
AB  - Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia
T1  - Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji
EP  - 107
IS  - 2
SP  - 97
VL  - 33
DO  - 10.2298/PIF1802097V
ER  - 

@article{
author = "Vojvodić, Mira and Tanović, Brankica and Mihajlović, Milica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2018",
abstract = "Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet., Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia, Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji",
pages = "107-97",
number = "2",
volume = "33",
doi = "10.2298/PIF1802097V"
}

Vojvodić, M., Tanović, B., Mihajlović, M., Mitrović, P., Vico, I.,& Bulajić, A.. (2018). Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(2), 97-107.
https://doi.org/10.2298/PIF1802097V

Vojvodić M, Tanović B, Mihajlović M, Mitrović P, Vico I, Bulajić A. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina. 2018;33(2):97-107.
doi:10.2298/PIF1802097V .

Vojvodić, Mira, Tanović, Brankica, Mihajlović, Milica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia" in Pesticidi i fitomedicina, 33, no. 2 (2018):97-107,
https://doi.org/10.2298/PIF1802097V . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Krstić, Branka
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1765
AB  - A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in sub-group N-1 with other European isolates of necrotic strains.
AB  - Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Intenzitet zaraze bio je različit i kretao se od 20% do 60%, u zavisnosti od ispitivane godine i lokaliteta gajenja paprike. Serološkim testiranjem uzoraka paprike prikupljenih u više lokaliteta u Srbiji, tokom 2009 i 2010. godine detektovani su Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), pri čemu je PVY bio dominantan. Specifičnim prajmerima PVYc/PVYd uz upotrebu RT-PCR metode umnožen je deo genoma od oko 975 bp koji kodira P1 protein. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank bazu podataka, gde su im dodeljeni pristupni brojevi PL-28-09 (KC288142), PL-15-09 (KC288143), PL-3-10 (KC288144), PL-108-10 (KC288144). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,8-100%. Ispitivane sekvence PVY izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Slovenije, Hrvatske, Nemačke i izolatom duvana iz Srbije. Svi ispitivani izolati sa paprike poreklom iz Srbije grupisani su u subklaster nekrotičnih izolata poreklom iz Evrope.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - Occurrence and diversity of viruses infecting pepper in Serbia
T1  - Prisustvo i rasprostranjenost virusa paprike u Srbiji
EP  - 155
IS  - 46
SP  - 141
VL  - 23
DO  - 10.5937/AASer1846141M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Stanković, Ivana and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Krstić, Branka",
year = "2018",
abstract = "A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in sub-group N-1 with other European isolates of necrotic strains., Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Intenzitet zaraze bio je različit i kretao se od 20% do 60%, u zavisnosti od ispitivane godine i lokaliteta gajenja paprike. Serološkim testiranjem uzoraka paprike prikupljenih u više lokaliteta u Srbiji, tokom 2009 i 2010. godine detektovani su Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), pri čemu je PVY bio dominantan. Specifičnim prajmerima PVYc/PVYd uz upotrebu RT-PCR metode umnožen je deo genoma od oko 975 bp koji kodira P1 protein. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank bazu podataka, gde su im dodeljeni pristupni brojevi PL-28-09 (KC288142), PL-15-09 (KC288143), PL-3-10 (KC288144), PL-108-10 (KC288144). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,8-100%. Ispitivane sekvence PVY izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Slovenije, Hrvatske, Nemačke i izolatom duvana iz Srbije. Svi ispitivani izolati sa paprike poreklom iz Srbije grupisani su u subklaster nekrotičnih izolata poreklom iz Evrope.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Occurrence and diversity of viruses infecting pepper in Serbia, Prisustvo i rasprostranjenost virusa paprike u Srbiji",
pages = "155-141",
number = "46",
volume = "23",
doi = "10.5937/AASer1846141M"
}

Milošević, D., Ignjatov, M., Stanković, I., Nikolić, Z., Gvozdanović-Varga, J.,& Krstić, B.. (2018). Occurrence and diversity of viruses infecting pepper in Serbia. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 23(46), 141-155.
https://doi.org/10.5937/AASer1846141M

Milošević D, Ignjatov M, Stanković I, Nikolić Z, Gvozdanović-Varga J, Krstić B. Occurrence and diversity of viruses infecting pepper in Serbia. in Acta agriculturae Serbica. 2018;23(46):141-155.
doi:10.5937/AASer1846141M .

Milošević, Dragana, Ignjatov, Maja, Stanković, Ivana, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Krstić, Branka, "Occurrence and diversity of viruses infecting pepper in Serbia" in Acta agriculturae Serbica, 23, no. 46 (2018):141-155,
https://doi.org/10.5937/AASer1846141M . .

TY  - JOUR
AU  - Krstić, Branka
AU  - Stanković, Ivana
AU  - Milošević, Dragana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1626
AB  - The number of virus species infecting pepper crops and their incidences has increased considerably over the past 30 years. This is probably due to a combination of factors, including the expansion and intensification of pepper cultivation, the increased global trade of plant material carrying viruses and vectors to new locations, and climate change expanding the geographic range suitable for the viruses and vectors. About 68 viruses are known to infect peppers worldwide. Most pepper viruses are distributed worldwide with the exception of viruses that have been reported only in certain geographic areas. Virus-infected peppers generally cxhibit a variety of symptoms, the most common of which are mosaic, mottle, necrosis and leaf distortion with consequent reductions in crop growth and yield. More than half of known pepper viruses are transmitted by aphids, and the other common pepper viruses are transmitted by whiteflies, thrips, or by contact and/or through the soil. In Serbia, the presence of five viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Tomato spotted wilt orthotospovirus (TSWV), Alfalfa mosaic virus (AMV) and Tobacco mosaic virus (TMV), with the prevalence of CMV and PVY. Some of these viruses cause considerable yield losses. For each economically important virus in Serbia, control measures are reported here.
AB  - Brojnost virusa koji zaražavaju papriku, kao i učestalost njihove pojave, značajno se povećala poslednjih 30 godina. Uzrok tome je, verovatno, kombinacija različitih faktora, kao što su ekspanzija i intenzivnije gajenje paprike, povećan obim međunarodne trgovine biljnog materijala čime je povećan rizik unošenja virusa i njihovih vektora u nove regione sveta i lokalitete, kao i klimatske promene koje odgovaraju širenju virusa i njihovih vektora. Do sada je utvrđeno da 68 virusa može da zarazi papriku. Većina virusa paprike je raširena celim svetom, ali ima i izuzetaka, koji su prisutni samo u određenim delovima sveta. Virusi paprike izazivaju ispoljavanje različitih tipova simptoma, a najčešći su mozaik, šarenilo i deformacija lišća koji imaju za posledicu smanjenje porasta biljaka i prinosa. Više od polovine virusa paprike se prenosi biljnim vašima, a drugi česti virusi se prenose leptirastim vašima, tripsima ili mehanički. U Srbiji je utvrđeno prisustvo pet virusa: virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus bronzavosti paradajza (Tomato spotted wilt orthotospovirus, TSWV), virus mozaika lucerke (Alfalfa mosaic virus, AMV) i virus mozaika duvana (Tobacco mosaic virus, TMV), s tim što su prevalentni CMV i PVY. Neki od tih virusa prouzrokuju značajne gubitke u prinosu. Za svaki ekonomski važan virus u Srbiji navedene su mere kontrole.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Economically important virus disease of pepper in Serbia
T1  - Ekonomski značajne viroze paprike u Srbiji
EP  - 627
IS  - 6
SP  - 610
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1626
ER  - 

@article{
author = "Krstić, Branka and Stanković, Ivana and Milošević, Dragana and Vučurović, Ana and Zečević, Katarina",
year = "2017",
abstract = "The number of virus species infecting pepper crops and their incidences has increased considerably over the past 30 years. This is probably due to a combination of factors, including the expansion and intensification of pepper cultivation, the increased global trade of plant material carrying viruses and vectors to new locations, and climate change expanding the geographic range suitable for the viruses and vectors. About 68 viruses are known to infect peppers worldwide. Most pepper viruses are distributed worldwide with the exception of viruses that have been reported only in certain geographic areas. Virus-infected peppers generally cxhibit a variety of symptoms, the most common of which are mosaic, mottle, necrosis and leaf distortion with consequent reductions in crop growth and yield. More than half of known pepper viruses are transmitted by aphids, and the other common pepper viruses are transmitted by whiteflies, thrips, or by contact and/or through the soil. In Serbia, the presence of five viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Tomato spotted wilt orthotospovirus (TSWV), Alfalfa mosaic virus (AMV) and Tobacco mosaic virus (TMV), with the prevalence of CMV and PVY. Some of these viruses cause considerable yield losses. For each economically important virus in Serbia, control measures are reported here., Brojnost virusa koji zaražavaju papriku, kao i učestalost njihove pojave, značajno se povećala poslednjih 30 godina. Uzrok tome je, verovatno, kombinacija različitih faktora, kao što su ekspanzija i intenzivnije gajenje paprike, povećan obim međunarodne trgovine biljnog materijala čime je povećan rizik unošenja virusa i njihovih vektora u nove regione sveta i lokalitete, kao i klimatske promene koje odgovaraju širenju virusa i njihovih vektora. Do sada je utvrđeno da 68 virusa može da zarazi papriku. Većina virusa paprike je raširena celim svetom, ali ima i izuzetaka, koji su prisutni samo u određenim delovima sveta. Virusi paprike izazivaju ispoljavanje različitih tipova simptoma, a najčešći su mozaik, šarenilo i deformacija lišća koji imaju za posledicu smanjenje porasta biljaka i prinosa. Više od polovine virusa paprike se prenosi biljnim vašima, a drugi česti virusi se prenose leptirastim vašima, tripsima ili mehanički. U Srbiji je utvrđeno prisustvo pet virusa: virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus bronzavosti paradajza (Tomato spotted wilt orthotospovirus, TSWV), virus mozaika lucerke (Alfalfa mosaic virus, AMV) i virus mozaika duvana (Tobacco mosaic virus, TMV), s tim što su prevalentni CMV i PVY. Neki od tih virusa prouzrokuju značajne gubitke u prinosu. Za svaki ekonomski važan virus u Srbiji navedene su mere kontrole.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Economically important virus disease of pepper in Serbia, Ekonomski značajne viroze paprike u Srbiji",
pages = "627-610",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1626"
}

Krstić, B., Stanković, I., Milošević, D., Vučurović, A.,& Zečević, K.. (2017). Economically important virus disease of pepper in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 610-627.
https://hdl.handle.net/21.15107/rcub_fiver_1626

Krstić B, Stanković I, Milošević D, Vučurović A, Zečević K. Economically important virus disease of pepper in Serbia. in Biljni lekar. 2017;45(6):610-627.
https://hdl.handle.net/21.15107/rcub_fiver_1626 .

Krstić, Branka, Stanković, Ivana, Milošević, Dragana, Vučurović, Ana, Zečević, Katarina, "Economically important virus disease of pepper in Serbia" in Biljni lekar, 45, no. 6 (2017):610-627,
https://hdl.handle.net/21.15107/rcub_fiver_1626 .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Krstić, Branka
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1621
AB  - A two-year investigation (2009-2010) of the presence and distribution of pepper viruses in Serbia revealed that viruses occur each year in open-field pepper production. Serological analyses of collected pepper samples using DAS-ELISA test detected the presence of four viruses: Potato virus Y(PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Pepper mild mottle virus (PMMoV), which occurred in single or mixed infections. In 2009 the most frequent was PVY (51.21%), while in 2010 CMV was prevalent (50%). Survey of pepper crops in different growing regions indicated the occurrence of a number of symptoms on leaves, stem, flowers and fruit, which resembled those of virus infection, but it was not possible to determine their association with detected virus. Molecular detection of CMV was performed by RT-PCR using specific primers CMV Au1u/Au2d that flank the AMV coat protein gene, as well as part of 5' and 3' non-coding region of subgenome RNA4. Amplified fragments were sequenced, deposited in the GenBank, and assigned by accession numbers, KC288146 (PL-25-09), KC288147 (PL-43-09) and KC288148 (PL-52-09). Sequence analysis, conducted with MEGA5 software, revealed 99,2-99,5% nt identity between the three Serbian CMV isolates from pepper. The sequences of CMV isolates from Serbia share the highest nucleotide and amino acid identity with isolates from America, Australia, Spain and Serbia.
AB  - Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Serološkim analizama sakupljenih uzoraka paprike primenom DAS-ELISA testa dokazano je prisustvo četiri virusa: Potato virus Y (PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) i Pepper mild mottle virus (PMMoV), i to kako u pojedinačnim tako i u mešanim infekcijama. Tokom 2009. godine najčešće detektovan virus bio je PVY (51,21%), dok je 2010. godine prevalentan virus bio CMV (50%). Pregledom useva paprike u različitim lokalitetima gajenja zabeležena je pojava niza simptoma na lišću, stablu, cvetovima i plodovima, koji su upućivali na virusnu zarazu, ali nije bilo moguće utvrditi njihovu povezanost sa detektovanim virusom. Molekularna detekcija CMV kod tri odabrana izolata obavljena je RT-PCR metodom korišćenjem specifičnih prajmera CMV Au1u/Au2d, koji omogućavaju umnožavanje gena za protein omotača i dela 5' i 3' neprepisujućeg regiona subgenomne RNA 4. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank, gde su im dodeljeni pristupni brojevi, KC288146 (PL-25-09), KC288147 (PL-43-09) i KC288148 (PL-52-09). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu, utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,2-99,5%. Ispitivane sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Amerike, Australije, Španije i Srbije.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Presence and distribution of pepper viruses in Serbia
T1  - Prisustvo i rasprostranjenost virusa paprike u Srbiji
EP  - 656
IS  - 6
SP  - 647
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1621
ER  - 

@article{
author = "Milošević, Dragana and Stanković, Ivana and Ignjatov, Maja and Nikolić, Zorica and Krstić, Branka",
year = "2017",
abstract = "A two-year investigation (2009-2010) of the presence and distribution of pepper viruses in Serbia revealed that viruses occur each year in open-field pepper production. Serological analyses of collected pepper samples using DAS-ELISA test detected the presence of four viruses: Potato virus Y(PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Pepper mild mottle virus (PMMoV), which occurred in single or mixed infections. In 2009 the most frequent was PVY (51.21%), while in 2010 CMV was prevalent (50%). Survey of pepper crops in different growing regions indicated the occurrence of a number of symptoms on leaves, stem, flowers and fruit, which resembled those of virus infection, but it was not possible to determine their association with detected virus. Molecular detection of CMV was performed by RT-PCR using specific primers CMV Au1u/Au2d that flank the AMV coat protein gene, as well as part of 5' and 3' non-coding region of subgenome RNA4. Amplified fragments were sequenced, deposited in the GenBank, and assigned by accession numbers, KC288146 (PL-25-09), KC288147 (PL-43-09) and KC288148 (PL-52-09). Sequence analysis, conducted with MEGA5 software, revealed 99,2-99,5% nt identity between the three Serbian CMV isolates from pepper. The sequences of CMV isolates from Serbia share the highest nucleotide and amino acid identity with isolates from America, Australia, Spain and Serbia., Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Serološkim analizama sakupljenih uzoraka paprike primenom DAS-ELISA testa dokazano je prisustvo četiri virusa: Potato virus Y (PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) i Pepper mild mottle virus (PMMoV), i to kako u pojedinačnim tako i u mešanim infekcijama. Tokom 2009. godine najčešće detektovan virus bio je PVY (51,21%), dok je 2010. godine prevalentan virus bio CMV (50%). Pregledom useva paprike u različitim lokalitetima gajenja zabeležena je pojava niza simptoma na lišću, stablu, cvetovima i plodovima, koji su upućivali na virusnu zarazu, ali nije bilo moguće utvrditi njihovu povezanost sa detektovanim virusom. Molekularna detekcija CMV kod tri odabrana izolata obavljena je RT-PCR metodom korišćenjem specifičnih prajmera CMV Au1u/Au2d, koji omogućavaju umnožavanje gena za protein omotača i dela 5' i 3' neprepisujućeg regiona subgenomne RNA 4. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank, gde su im dodeljeni pristupni brojevi, KC288146 (PL-25-09), KC288147 (PL-43-09) i KC288148 (PL-52-09). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu, utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,2-99,5%. Ispitivane sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Amerike, Australije, Španije i Srbije.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Presence and distribution of pepper viruses in Serbia, Prisustvo i rasprostranjenost virusa paprike u Srbiji",
pages = "656-647",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1621"
}

Milošević, D., Stanković, I., Ignjatov, M., Nikolić, Z.,& Krstić, B.. (2017). Presence and distribution of pepper viruses in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 647-656.
https://hdl.handle.net/21.15107/rcub_fiver_1621

Milošević D, Stanković I, Ignjatov M, Nikolić Z, Krstić B. Presence and distribution of pepper viruses in Serbia. in Biljni lekar. 2017;45(6):647-656.
https://hdl.handle.net/21.15107/rcub_fiver_1621 .

Milošević, Dragana, Stanković, Ivana, Ignjatov, Maja, Nikolić, Zorica, Krstić, Branka, "Presence and distribution of pepper viruses in Serbia" in Biljni lekar, 45, no. 6 (2017):647-656,
https://hdl.handle.net/21.15107/rcub_fiver_1621 .

TY  - CONF
AU  - Vuković, Slavica
AU  - Krnjajić, Slobodan
AU  - Inđić, Dušanka
AU  - Gvozdenac, Sonja
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1597
AB  - Aphids occur regularly in apple orchards and cause severe damages. This research aimed to assess the efficacy of biological - azadirachtin (10 g a.i. L-1) 0.2% and chemical insecticides - chlorpyrifos + cypermethrin (50 + 50 g a.i. L-1) 0.1%, against Aphis pomi in apple orchard. The experiments were performed in 2013 and 2014 in Novi Slankamen, Celarevo and Bela Crkva (Serbia, Vojvodina) according to standard EPPO methods, on cultivar 'Golden Delicious'. Treatment was performed twice with azadirachtin in seven days interval and once with chlorpyrifos + cypermethrin. Three assessments were made: before the treatment, immediately after the second treatment and 10 days after the second treatment, on 10 previously marked shoots per replication, by counting the number of aphids. Efficacy (E%) was calculated according to Abbott. In 2013, the number of aphids in Novi Slankamen and Celarevo ranged from 58.7 to 70.0 before the treatment, and after seven days (26.5 to 33.5) was significantly lower than in the control (99.5 to 133.7). Insecticides efficacy was 66.3 to 79.6%. Ten days after the second treatment, the number of aphids (9.75 to 10.7) was significantly lower than in the control (135.5 to 148) and insecticides efficacy was 92.2 to 93.9%. In Celarevo and Bela Crkva in 2014 the average number of aphids before the treatment was 71.7 to 81.2 and after seven days (34.5 to 43) was significantly lower compared to the control (127.7 to 145.2) and the insecticides efficacy was 66.3 to 76.2%. The number of aphids (16.2 to 18.2), 10 days after the second treatment was statistically significantly lower compared to the control (160.5 to 160.7) and insecticides efficacy was 88.6 to 90.1%. Efficacy of tested insecticides was on the same level of significance thus it can be concluded that azadirchtin can ensure adequate protection from A. pomi in apple orchards.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - III Balkan Symposium on Fruit Growing
T1  - Possibility of Aphis pomi control in apple orchards with azadirachtin
EP  - 731
SP  - 727
VL  - 1139
DO  - 10.17660/ActaHortic.2016.1139.124
ER  - 

@conference{
author = "Vuković, Slavica and Krnjajić, Slobodan and Inđić, Dušanka and Gvozdenac, Sonja",
year = "2016",
abstract = "Aphids occur regularly in apple orchards and cause severe damages. This research aimed to assess the efficacy of biological - azadirachtin (10 g a.i. L-1) 0.2% and chemical insecticides - chlorpyrifos + cypermethrin (50 + 50 g a.i. L-1) 0.1%, against Aphis pomi in apple orchard. The experiments were performed in 2013 and 2014 in Novi Slankamen, Celarevo and Bela Crkva (Serbia, Vojvodina) according to standard EPPO methods, on cultivar 'Golden Delicious'. Treatment was performed twice with azadirachtin in seven days interval and once with chlorpyrifos + cypermethrin. Three assessments were made: before the treatment, immediately after the second treatment and 10 days after the second treatment, on 10 previously marked shoots per replication, by counting the number of aphids. Efficacy (E%) was calculated according to Abbott. In 2013, the number of aphids in Novi Slankamen and Celarevo ranged from 58.7 to 70.0 before the treatment, and after seven days (26.5 to 33.5) was significantly lower than in the control (99.5 to 133.7). Insecticides efficacy was 66.3 to 79.6%. Ten days after the second treatment, the number of aphids (9.75 to 10.7) was significantly lower than in the control (135.5 to 148) and insecticides efficacy was 92.2 to 93.9%. In Celarevo and Bela Crkva in 2014 the average number of aphids before the treatment was 71.7 to 81.2 and after seven days (34.5 to 43) was significantly lower compared to the control (127.7 to 145.2) and the insecticides efficacy was 66.3 to 76.2%. The number of aphids (16.2 to 18.2), 10 days after the second treatment was statistically significantly lower compared to the control (160.5 to 160.7) and insecticides efficacy was 88.6 to 90.1%. Efficacy of tested insecticides was on the same level of significance thus it can be concluded that azadirchtin can ensure adequate protection from A. pomi in apple orchards.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "III Balkan Symposium on Fruit Growing",
title = "Possibility of Aphis pomi control in apple orchards with azadirachtin",
pages = "731-727",
volume = "1139",
doi = "10.17660/ActaHortic.2016.1139.124"
}

Vuković, S., Krnjajić, S., Inđić, D.,& Gvozdenac, S.. (2016). Possibility of Aphis pomi control in apple orchards with azadirachtin. in III Balkan Symposium on Fruit Growing
Int Soc Horticultural Science, Leuven 1., 1139, 727-731.
https://doi.org/10.17660/ActaHortic.2016.1139.124

Vuković S, Krnjajić S, Inđić D, Gvozdenac S. Possibility of Aphis pomi control in apple orchards with azadirachtin. in III Balkan Symposium on Fruit Growing. 2016;1139:727-731.
doi:10.17660/ActaHortic.2016.1139.124 .

Vuković, Slavica, Krnjajić, Slobodan, Inđić, Dušanka, Gvozdenac, Sonja, "Possibility of Aphis pomi control in apple orchards with azadirachtin" in III Balkan Symposium on Fruit Growing, 1139 (2016):727-731,
https://doi.org/10.17660/ActaHortic.2016.1139.124 . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Marjanović-Jeromela, Ana
AU  - Krstić, Branka
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1516
AB  - A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates.
AB  - Tokom 2014. godine, sa šest lokaliteta gajenja uljane repice, ukupno je sakupljeno 86 uzorka koji su serološki testirani na prisustvo virusa žutice postrne repe [Turnip yellow virus (TuYV)], virusa mozaika karfiola [Cauliflower mosaic virus (CaMV)] i virusa mozaika postrne repe [Turnip mosaic virus (TuMV)], korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV dokazano je u 60 (69.77%) prikupljenih uzoraka, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka uljane repice poreklom iz različitih lokaliteta koji su uspešno preneti vašima na tri različite test biljke, čime je potvrđena infektivna priroda oboljenja. Prisustvo TuYV u ELISA pozitivním uzorcima je potvrđeno korišćenjem RT-PCR i sekvencioniranjem. Poređenjem dobijenih sekvenci sa sekvencama dostupnih u GenBank bazi podataka, potvrđena je autentičnost serološki detektovanih virusa. Na osnovu sekvenci P0 gena izolata TuYV, utvrđena je pripadnost ispitivanih izolata iz uljane repice grupi sa ostalim TuYV izolatima, koji su jasno razdvojeni od Beet western yellows virus (BWYV) izolata.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia
T1  - Prisustvo virusa žutice postrne repe na uljanoj repici (Brassica napus L.) u Srbiji
EP  - 44
IS  - 1-2
SP  - 37
VL  - 31
DO  - 10.2298/PIF1602037M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Stanković, Ivana and Bulajić, Aleksandra and Marjanović-Jeromela, Ana and Krstić, Branka",
year = "2016",
abstract = "A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates., Tokom 2014. godine, sa šest lokaliteta gajenja uljane repice, ukupno je sakupljeno 86 uzorka koji su serološki testirani na prisustvo virusa žutice postrne repe [Turnip yellow virus (TuYV)], virusa mozaika karfiola [Cauliflower mosaic virus (CaMV)] i virusa mozaika postrne repe [Turnip mosaic virus (TuMV)], korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV dokazano je u 60 (69.77%) prikupljenih uzoraka, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka uljane repice poreklom iz različitih lokaliteta koji su uspešno preneti vašima na tri različite test biljke, čime je potvrđena infektivna priroda oboljenja. Prisustvo TuYV u ELISA pozitivním uzorcima je potvrđeno korišćenjem RT-PCR i sekvencioniranjem. Poređenjem dobijenih sekvenci sa sekvencama dostupnih u GenBank bazi podataka, potvrđena je autentičnost serološki detektovanih virusa. Na osnovu sekvenci P0 gena izolata TuYV, utvrđena je pripadnost ispitivanih izolata iz uljane repice grupi sa ostalim TuYV izolatima, koji su jasno razdvojeni od Beet western yellows virus (BWYV) izolata.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia, Prisustvo virusa žutice postrne repe na uljanoj repici (Brassica napus L.) u Srbiji",
pages = "44-37",
number = "1-2",
volume = "31",
doi = "10.2298/PIF1602037M"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Stanković, I., Bulajić, A., Marjanović-Jeromela, A.,& Krstić, B.. (2016). The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 31(1-2), 37-44.
https://doi.org/10.2298/PIF1602037M

Milošević D, Ignjatov M, Nikolić Z, Stanković I, Bulajić A, Marjanović-Jeromela A, Krstić B. The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia. in Pesticidi i fitomedicina. 2016;31(1-2):37-44.
doi:10.2298/PIF1602037M .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Stanković, Ivana, Bulajić, Aleksandra, Marjanović-Jeromela, Ana, Krstić, Branka, "The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia" in Pesticidi i fitomedicina, 31, no. 1-2 (2016):37-44,
https://doi.org/10.2298/PIF1602037M . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Krstić, Branka
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1407
AB  - During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Detection and molecular characterization of pepper mild mottle virus in Serbia
EP  - 663
IS  - 2
SP  - 651
VL  - 47
DO  - 10.2298/GENSR1502651M
ER  - 

@article{
author = "Milošević, Dragana and Stanković, Ivana and Bulajić, Aleksandra and Ignjatov, Maja and Nikolić, Zorica and Petrović, Gordana and Krstić, Branka",
year = "2015",
abstract = "During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Detection and molecular characterization of pepper mild mottle virus in Serbia",
pages = "663-651",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502651M"
}

Milošević, D., Stanković, I., Bulajić, A., Ignjatov, M., Nikolić, Z., Petrović, G.,& Krstić, B.. (2015). Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(2), 651-663.
https://doi.org/10.2298/GENSR1502651M

Milošević D, Stanković I, Bulajić A, Ignjatov M, Nikolić Z, Petrović G, Krstić B. Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika-Belgrade. 2015;47(2):651-663.
doi:10.2298/GENSR1502651M .

Milošević, Dragana, Stanković, Ivana, Bulajić, Aleksandra, Ignjatov, Maja, Nikolić, Zorica, Petrović, Gordana, Krstić, Branka, "Detection and molecular characterization of pepper mild mottle virus in Serbia" in Genetika-Belgrade, 47, no. 2 (2015):651-663,
https://doi.org/10.2298/GENSR1502651M . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Tamindžić, Gordana
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1477
AB  - During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II.
AB  - Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia
T1  - Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji
EP  - 73
IS  - 2
SP  - 67
VL  - 52
DO  - 10.5937/ratpov52-7814
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tamindžić, Gordana and Stanković, Ivana and Krstić, Branka",
year = "2015",
abstract = "During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II., Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia, Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji",
pages = "73-67",
number = "2",
volume = "52",
doi = "10.5937/ratpov52-7814"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Gvozdanović-Varga, J., Tamindžić, G., Stanković, I.,& Krstić, B.. (2015). The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 52(2), 67-73.
https://doi.org/10.5937/ratpov52-7814

Milošević D, Ignjatov M, Nikolić Z, Gvozdanović-Varga J, Tamindžić G, Stanković I, Krstić B. The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2015;52(2):67-73.
doi:10.5937/ratpov52-7814 .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tamindžić, Gordana, Stanković, Ivana, Krstić, Branka, "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 52, no. 2 (2015):67-73,
https://doi.org/10.5937/ratpov52-7814 . .