TY  - JOUR
AU  - Vojvodić, Mira
AU  - Lazić, Dejan
AU  - Mitrović, Petar
AU  - Tanović, Brankica
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1904
AB  - Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples.
AB  - Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet
T1  - Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji
EP  - 29
IS  - 1
SP  - 19
VL  - 34
DO  - 10.2298/PIF1901019V
ER  - 

@article{
author = "Vojvodić, Mira and Lazić, Dejan and Mitrović, Petar and Tanović, Brankica and Vico, Ivana and Bulajić, Aleksandra",
year = "2019",
abstract = "Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples., Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet, Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji",
pages = "29-19",
number = "1",
volume = "34",
doi = "10.2298/PIF1901019V"
}

Vojvodić, M., Lazić, D., Mitrović, P., Tanović, B., Vico, I.,& Bulajić, A.. (2019). Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(1), 19-29.
https://doi.org/10.2298/PIF1901019V

Vojvodić M, Lazić D, Mitrović P, Tanović B, Vico I, Bulajić A. Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina. 2019;34(1):19-29.
doi:10.2298/PIF1901019V .

Vojvodić, Mira, Lazić, Dejan, Mitrović, Petar, Tanović, Brankica, Vico, Ivana, Bulajić, Aleksandra, "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet" in Pesticidi i fitomedicina, 34, no. 1 (2019):19-29,
https://doi.org/10.2298/PIF1901019V . .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mihajlović, Milica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1759
AB  - Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet.
AB  - Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia
T1  - Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji
EP  - 107
IS  - 2
SP  - 97
VL  - 33
DO  - 10.2298/PIF1802097V
ER  - 

@article{
author = "Vojvodić, Mira and Tanović, Brankica and Mihajlović, Milica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2018",
abstract = "Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet., Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia, Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji",
pages = "107-97",
number = "2",
volume = "33",
doi = "10.2298/PIF1802097V"
}

Vojvodić, M., Tanović, B., Mihajlović, M., Mitrović, P., Vico, I.,& Bulajić, A.. (2018). Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(2), 97-107.
https://doi.org/10.2298/PIF1802097V

Vojvodić M, Tanović B, Mihajlović M, Mitrović P, Vico I, Bulajić A. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina. 2018;33(2):97-107.
doi:10.2298/PIF1802097V .

Vojvodić, Mira, Tanović, Brankica, Mihajlović, Milica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia" in Pesticidi i fitomedicina, 33, no. 2 (2018):97-107,
https://doi.org/10.2298/PIF1802097V . .