Mihajlović, Milica

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  • Mihajlović, Milica (2)
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Author's Bibliography

Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia

Vojvodić, Mira; Tanović, Brankica; Mihajlović, Milica; Mitrović, Petar; Vico, Ivana; Bulajić, Aleksandra

(Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd, 2018)

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mihajlović, Milica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1759
AB  - Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet.
AB  - Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia
T1  - Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji
EP  - 107
IS  - 2
SP  - 97
VL  - 33
DO  - 10.2298/PIF1802097V
ER  - 
@article{
author = "Vojvodić, Mira and Tanović, Brankica and Mihajlović, Milica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2018",
abstract = "Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet., Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia, Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji",
pages = "107-97",
number = "2",
volume = "33",
doi = "10.2298/PIF1802097V"
}
Vojvodić, M., Tanović, B., Mihajlović, M., Mitrović, P., Vico, I.,& Bulajić, A.. (2018). Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(2), 97-107.
https://doi.org/10.2298/PIF1802097V
Vojvodić M, Tanović B, Mihajlović M, Mitrović P, Vico I, Bulajić A. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina. 2018;33(2):97-107.
doi:10.2298/PIF1802097V .
Vojvodić, Mira, Tanović, Brankica, Mihajlović, Milica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia" in Pesticidi i fitomedicina, 33, no. 2 (2018):97-107,
https://doi.org/10.2298/PIF1802097V . .
5

Morphological and ecological features as differentiation criteria for Colletotrichum species

Grahovac, Mila; Inđić, Dušanka; Vuković, Slavica; Hrustić, Jovana; Gvozdenac, Sonja; Mihajlović, Milica; Tanović, Brankica

(Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University, 2012)

TY  - JOUR
AU  - Grahovac, Mila
AU  - Inđić, Dušanka
AU  - Vuković, Slavica
AU  - Hrustić, Jovana
AU  - Gvozdenac, Sonja
AU  - Mihajlović, Milica
AU  - Tanović, Brankica
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1173
AB  - Colletotrichum acutatum and Colletotrichum gloeosporioides are important post harvest apple fruit pathogens in temperate regions. Seven Colletotrichum isolates obtained from apple fruits with anthracnose symptoms, and two reference isolates C. gloeosporioides AVO 37 4B (avocado, Israel) and C. actuatum TUT 137 A (strawberry, Israel) were investigated. Pathogenicity of all nine isolates was demonstrated on wound-inoculated apple fruits by fulfilling Koch's postulates. Investigated morphological characteristics involved shape, colour and margin of the colony, as well as conidial shape. Observed ecological parameters were growth and sporulation of isolates after 4, 7 and 11 days of incubation at temperatures of 15, 20, 23, 25, 27, 30, 32 and 35 degrees C and after 10 days of incubation under conditions of day light regime and dark. The isolates were identified by PCR using species-specific primers. The data regarding growth were processed by factorial and one way ANOVA using software Statistica 10. All seven isolates obtained from apple fruits and reference C. acutatum isolate formed velvety, gray colonies with slightly wavy margin, and one celled fusiform conidia on PDA medium. Mycelium of the reference isolate C. gloeosporioides AVO 37 4B was snow-white and conidia were cylindrical. Maximum mycelial growth rate for isolates obtained from apple fruits was at temperature of 23 degrees C, for reference isolate C. acutatum at 27 degrees C and for C. gloeosporioides at 23-27 degrees C. Temperature of 35 degrees C proved to be restrictive for C. acutatum isolates. Isolate C. gloeosporioides had the fastest growth rate compared to all other isolates. Using PCR with species-specific primers all eight isolates were identified as C. acutatum (fragments sized 490 bp were amplified), and one as C. gloeosporioides (fragments sized 450 bp were amplified). The results suggest that differentiation between the two Colletotrichum species based on several morphological and ecological parameters is possible.
PB  - Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University
T2  - Zemdirbyste
T1  - Morphological and ecological features as differentiation criteria for Colletotrichum species
EP  - 196
IS  - 2
SP  - 189
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1173
ER  - 
@article{
author = "Grahovac, Mila and Inđić, Dušanka and Vuković, Slavica and Hrustić, Jovana and Gvozdenac, Sonja and Mihajlović, Milica and Tanović, Brankica",
year = "2012",
abstract = "Colletotrichum acutatum and Colletotrichum gloeosporioides are important post harvest apple fruit pathogens in temperate regions. Seven Colletotrichum isolates obtained from apple fruits with anthracnose symptoms, and two reference isolates C. gloeosporioides AVO 37 4B (avocado, Israel) and C. actuatum TUT 137 A (strawberry, Israel) were investigated. Pathogenicity of all nine isolates was demonstrated on wound-inoculated apple fruits by fulfilling Koch's postulates. Investigated morphological characteristics involved shape, colour and margin of the colony, as well as conidial shape. Observed ecological parameters were growth and sporulation of isolates after 4, 7 and 11 days of incubation at temperatures of 15, 20, 23, 25, 27, 30, 32 and 35 degrees C and after 10 days of incubation under conditions of day light regime and dark. The isolates were identified by PCR using species-specific primers. The data regarding growth were processed by factorial and one way ANOVA using software Statistica 10. All seven isolates obtained from apple fruits and reference C. acutatum isolate formed velvety, gray colonies with slightly wavy margin, and one celled fusiform conidia on PDA medium. Mycelium of the reference isolate C. gloeosporioides AVO 37 4B was snow-white and conidia were cylindrical. Maximum mycelial growth rate for isolates obtained from apple fruits was at temperature of 23 degrees C, for reference isolate C. acutatum at 27 degrees C and for C. gloeosporioides at 23-27 degrees C. Temperature of 35 degrees C proved to be restrictive for C. acutatum isolates. Isolate C. gloeosporioides had the fastest growth rate compared to all other isolates. Using PCR with species-specific primers all eight isolates were identified as C. acutatum (fragments sized 490 bp were amplified), and one as C. gloeosporioides (fragments sized 450 bp were amplified). The results suggest that differentiation between the two Colletotrichum species based on several morphological and ecological parameters is possible.",
publisher = "Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University",
journal = "Zemdirbyste",
title = "Morphological and ecological features as differentiation criteria for Colletotrichum species",
pages = "196-189",
number = "2",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1173"
}
Grahovac, M., Inđić, D., Vuković, S., Hrustić, J., Gvozdenac, S., Mihajlović, M.,& Tanović, B.. (2012). Morphological and ecological features as differentiation criteria for Colletotrichum species. in Zemdirbyste
Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University., 99(2), 189-196.
https://hdl.handle.net/21.15107/rcub_fiver_1173
Grahovac M, Inđić D, Vuković S, Hrustić J, Gvozdenac S, Mihajlović M, Tanović B. Morphological and ecological features as differentiation criteria for Colletotrichum species. in Zemdirbyste. 2012;99(2):189-196.
https://hdl.handle.net/21.15107/rcub_fiver_1173 .
Grahovac, Mila, Inđić, Dušanka, Vuković, Slavica, Hrustić, Jovana, Gvozdenac, Sonja, Mihajlović, Milica, Tanović, Brankica, "Morphological and ecological features as differentiation criteria for Colletotrichum species" in Zemdirbyste, 99, no. 2 (2012):189-196,
https://hdl.handle.net/21.15107/rcub_fiver_1173 .
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