TY  - CONF
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2811
AB  - Waitea circinata Warcup & P.H.B. Talbot (multinuclear Rhizoctonia spp.) is a widespread, soil-borne plant pathogen causing root rot mainly of monocotyledonous plants. Among five described varieties, the most common and widespread is W. circinata var. zeae Toda, Mushika, T. Hayak., Tanaka, Tani & Hyakum. Besides monocotyledonous hosts, a natural infection of a limited number of dicotyledonous plants, including soybeans, beans, peas, sugar beets and carrots were recorded recently. During 2017, cabbage and oilseed rape plants showing symptoms of root and root neck rot, sampled at the localities of Futog and Rimski Šančevi (Serbia), were proved to be infected with W. circinata var. zeae. This was the first record of natural infection of plants from Brassicaceae family worldwide. Two selected isolates from cabbage (299-17) and oilseed rape (300-17) formed fast-growing orange colonies with red sclerotia and multinucleate hyphae with a characteristic branching pattern. The identification was further confirmed by BLAST analysis of sequences of ITS rDNA, RPB2, LSU and β-tubulin genomic regions. Koch’s postulates were fulfilled after inoculation of cabbage and oilseed rape seedlings, while an experimental host range was determined by inoculations of 14 different plant species from 8 families.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
T1  - Pathogenic potential of Waitea circinata var. zeae
EP  - 84
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2811
ER  - 

@conference{
author = "Vojvodić, Mira and Tanović, Brankica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2022",
abstract = "Waitea circinata Warcup & P.H.B. Talbot (multinuclear Rhizoctonia spp.) is a widespread, soil-borne plant pathogen causing root rot mainly of monocotyledonous plants. Among five described varieties, the most common and widespread is W. circinata var. zeae Toda, Mushika, T. Hayak., Tanaka, Tani & Hyakum. Besides monocotyledonous hosts, a natural infection of a limited number of dicotyledonous plants, including soybeans, beans, peas, sugar beets and carrots were recorded recently. During 2017, cabbage and oilseed rape plants showing symptoms of root and root neck rot, sampled at the localities of Futog and Rimski Šančevi (Serbia), were proved to be infected with W. circinata var. zeae. This was the first record of natural infection of plants from Brassicaceae family worldwide. Two selected isolates from cabbage (299-17) and oilseed rape (300-17) formed fast-growing orange colonies with red sclerotia and multinucleate hyphae with a characteristic branching pattern. The identification was further confirmed by BLAST analysis of sequences of ITS rDNA, RPB2, LSU and β-tubulin genomic regions. Koch’s postulates were fulfilled after inoculation of cabbage and oilseed rape seedlings, while an experimental host range was determined by inoculations of 14 different plant species from 8 families.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina",
title = "Pathogenic potential of Waitea circinata var. zeae",
pages = "84-83",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2811"
}

Vojvodić, M., Tanović, B., Mitrović, P., Vico, I.,& Bulajić, A.. (2022). Pathogenic potential of Waitea circinata var. zeae. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 83-84.
https://hdl.handle.net/21.15107/rcub_fiver_2811

Vojvodić M, Tanović B, Mitrović P, Vico I, Bulajić A. Pathogenic potential of Waitea circinata var. zeae. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina. 2022;:83-84.
https://hdl.handle.net/21.15107/rcub_fiver_2811 .

Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Pathogenic potential of Waitea circinata var. zeae" in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina (2022):83-84,
https://hdl.handle.net/21.15107/rcub_fiver_2811 .

TY  - CONF
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/4245
AB  - Kupus i uljana repica su dva vrlo značajna useva u našoj zemlji i njihova proizvodnja ugrožena je brojnim patogenim organizmima. Višejedarne anamorfne Rhizoctonia spp. sa teleomorfom opisanim kao Waitea circinata su biljni patogeni koji se dugo održavaju u zemljištu i rasprostranjeni su i vrlo štetni širom sveta. Od pet opisanih varijeteta W. circinata var. zeae je najrasprostranjeniji. Za sada poznat krug domaćina W. circinata var. zeae obuhvata monokotiledone i mali broj dikotiledonih biljaka, među kojima soju, pasulj, grašak, šećernu repu i mrkvu.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.
T1  - Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji
EP  - 20
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4245
ER  - 

@conference{
author = "Vojvodić, Mira and Tanović, Brankica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2021",
abstract = "Kupus i uljana repica su dva vrlo značajna useva u našoj zemlji i njihova proizvodnja ugrožena je brojnim patogenim organizmima. Višejedarne anamorfne Rhizoctonia spp. sa teleomorfom opisanim kao Waitea circinata su biljni patogeni koji se dugo održavaju u zemljištu i rasprostranjeni su i vrlo štetni širom sveta. Od pet opisanih varijeteta W. circinata var. zeae je najrasprostranjeniji. Za sada poznat krug domaćina W. circinata var. zeae obuhvata monokotiledone i mali broj dikotiledonih biljaka, među kojima soju, pasulj, grašak, šećernu repu i mrkvu.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.",
title = "Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji",
pages = "20-19",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4245"
}

Vojvodić, M., Tanović, B., Mitrović, P., Vico, I.,& Bulajić, A.. (2021). Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji. in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.
Beograd : Društvo za zaštitu bilja Srbije., 19-20.
https://hdl.handle.net/21.15107/rcub_fiver_4245

Vojvodić M, Tanović B, Mitrović P, Vico I, Bulajić A. Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji. in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021.. 2021;:19-20.
https://hdl.handle.net/21.15107/rcub_fiver_4245 .

Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji" in Zbornik rezimea radova, 16. Simpozijum o zaštiti bilja, Zlatibor, 22-25. novembar 2021. (2021):19-20,
https://hdl.handle.net/21.15107/rcub_fiver_4245 .

TY  - CONF
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Lazić, Dejan
AU  - Stevanović, Miloš
AU  - Bulajić, Aleksandra
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2373
AB  - Rhizoctonia spp. comprise soil-borne fungi widely distributed in temperate climate worldwide which are annually causing significant yield loses to a range of cultivated plants. A species status of Rhizoctonia spp. has not yet been established and the isolates are classified based on nuclei status as multinucleate and binucleate Rhizoctonia spp., and further divided into 13 anastomosis groups (AG) (AG-1-13) of multinucleate Rhizoctonia and 23 AG (AG-A-W) of binucleate Rhizoctonia. In this paper we are reporting morphological and molecular features of binucleate Rhizoctonia spp. AG-A causing black root rot on strawberry and multinucleate R. solani AG-2-2 causing root and crown rot of sugar beet. During 2015 and 2016 at several strawberry and sugar beet producing areas, plants expressing prominent root and crown necrosis and rot have been observed with disease incidence estimated at 20-30%.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
T1  - Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia
EP  - 32
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2373
ER  - 

@conference{
author = "Vojvodić, Mira and Tanović, Brankica and Mitrović, Petar and Vico, Ivana and Lazić, Dejan and Stevanović, Miloš and Bulajić, Aleksandra",
year = "2020",
abstract = "Rhizoctonia spp. comprise soil-borne fungi widely distributed in temperate climate worldwide which are annually causing significant yield loses to a range of cultivated plants. A species status of Rhizoctonia spp. has not yet been established and the isolates are classified based on nuclei status as multinucleate and binucleate Rhizoctonia spp., and further divided into 13 anastomosis groups (AG) (AG-1-13) of multinucleate Rhizoctonia and 23 AG (AG-A-W) of binucleate Rhizoctonia. In this paper we are reporting morphological and molecular features of binucleate Rhizoctonia spp. AG-A causing black root rot on strawberry and multinucleate R. solani AG-2-2 causing root and crown rot of sugar beet. During 2015 and 2016 at several strawberry and sugar beet producing areas, plants expressing prominent root and crown necrosis and rot have been observed with disease incidence estimated at 20-30%.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina",
title = "Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia",
pages = "32-31",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2373"
}

Vojvodić, M., Tanović, B., Mitrović, P., Vico, I., Lazić, D., Stevanović, M.,& Bulajić, A.. (2020). Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia. in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 31-32.
https://hdl.handle.net/21.15107/rcub_fiver_2373

Vojvodić M, Tanović B, Mitrović P, Vico I, Lazić D, Stevanović M, Bulajić A. Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia. in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina. 2020;:31-32.
https://hdl.handle.net/21.15107/rcub_fiver_2373 .

Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Lazić, Dejan, Stevanović, Miloš, Bulajić, Aleksandra, "Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia" in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina (2020):31-32,
https://hdl.handle.net/21.15107/rcub_fiver_2373 .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Lazić, Dejan
AU  - Mitrović, Petar
AU  - Tanović, Brankica
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1904
AB  - Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples.
AB  - Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet
T1  - Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji
EP  - 29
IS  - 1
SP  - 19
VL  - 34
DO  - 10.2298/PIF1901019V
ER  - 

@article{
author = "Vojvodić, Mira and Lazić, Dejan and Mitrović, Petar and Tanović, Brankica and Vico, Ivana and Bulajić, Aleksandra",
year = "2019",
abstract = "Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples., Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet, Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji",
pages = "29-19",
number = "1",
volume = "34",
doi = "10.2298/PIF1901019V"
}

Vojvodić, M., Lazić, D., Mitrović, P., Tanović, B., Vico, I.,& Bulajić, A.. (2019). Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(1), 19-29.
https://doi.org/10.2298/PIF1901019V

Vojvodić M, Lazić D, Mitrović P, Tanović B, Vico I, Bulajić A. Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina. 2019;34(1):19-29.
doi:10.2298/PIF1901019V .

Vojvodić, Mira, Lazić, Dejan, Mitrović, Petar, Tanović, Brankica, Vico, Ivana, Bulajić, Aleksandra, "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet" in Pesticidi i fitomedicina, 34, no. 1 (2019):19-29,
https://doi.org/10.2298/PIF1901019V . .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mihajlović, Milica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1759
AB  - Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet.
AB  - Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia
T1  - Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji
EP  - 107
IS  - 2
SP  - 97
VL  - 33
DO  - 10.2298/PIF1802097V
ER  - 

@article{
author = "Vojvodić, Mira and Tanović, Brankica and Mihajlović, Milica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2018",
abstract = "Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet., Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia, Molekularna identifikacija i karakterizacija dvojedarnih Rhizoctonia spp. prouzrokovača crne truleži korena jagode u Srbiji",
pages = "107-97",
number = "2",
volume = "33",
doi = "10.2298/PIF1802097V"
}

Vojvodić, M., Tanović, B., Mihajlović, M., Mitrović, P., Vico, I.,& Bulajić, A.. (2018). Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 33(2), 97-107.
https://doi.org/10.2298/PIF1802097V

Vojvodić M, Tanović B, Mihajlović M, Mitrović P, Vico I, Bulajić A. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia. in Pesticidi i fitomedicina. 2018;33(2):97-107.
doi:10.2298/PIF1802097V .

Vojvodić, Mira, Tanović, Brankica, Mihajlović, Milica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia" in Pesticidi i fitomedicina, 33, no. 2 (2018):97-107,
https://doi.org/10.2298/PIF1802097V . .

TY  - JOUR
AU  - Grahovac, Mila
AU  - Inđić, Dušanka
AU  - Vuković, Slavica
AU  - Hrustić, Jovana
AU  - Gvozdenac, Sonja
AU  - Mihajlović, Milica
AU  - Tanović, Brankica
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1173
AB  - Colletotrichum acutatum and Colletotrichum gloeosporioides are important post harvest apple fruit pathogens in temperate regions. Seven Colletotrichum isolates obtained from apple fruits with anthracnose symptoms, and two reference isolates C. gloeosporioides AVO 37 4B (avocado, Israel) and C. actuatum TUT 137 A (strawberry, Israel) were investigated. Pathogenicity of all nine isolates was demonstrated on wound-inoculated apple fruits by fulfilling Koch's postulates. Investigated morphological characteristics involved shape, colour and margin of the colony, as well as conidial shape. Observed ecological parameters were growth and sporulation of isolates after 4, 7 and 11 days of incubation at temperatures of 15, 20, 23, 25, 27, 30, 32 and 35 degrees C and after 10 days of incubation under conditions of day light regime and dark. The isolates were identified by PCR using species-specific primers. The data regarding growth were processed by factorial and one way ANOVA using software Statistica 10. All seven isolates obtained from apple fruits and reference C. acutatum isolate formed velvety, gray colonies with slightly wavy margin, and one celled fusiform conidia on PDA medium. Mycelium of the reference isolate C. gloeosporioides AVO 37 4B was snow-white and conidia were cylindrical. Maximum mycelial growth rate for isolates obtained from apple fruits was at temperature of 23 degrees C, for reference isolate C. acutatum at 27 degrees C and for C. gloeosporioides at 23-27 degrees C. Temperature of 35 degrees C proved to be restrictive for C. acutatum isolates. Isolate C. gloeosporioides had the fastest growth rate compared to all other isolates. Using PCR with species-specific primers all eight isolates were identified as C. acutatum (fragments sized 490 bp were amplified), and one as C. gloeosporioides (fragments sized 450 bp were amplified). The results suggest that differentiation between the two Colletotrichum species based on several morphological and ecological parameters is possible.
PB  - Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University
T2  - Zemdirbyste
T1  - Morphological and ecological features as differentiation criteria for Colletotrichum species
EP  - 196
IS  - 2
SP  - 189
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1173
ER  - 

@article{
author = "Grahovac, Mila and Inđić, Dušanka and Vuković, Slavica and Hrustić, Jovana and Gvozdenac, Sonja and Mihajlović, Milica and Tanović, Brankica",
year = "2012",
abstract = "Colletotrichum acutatum and Colletotrichum gloeosporioides are important post harvest apple fruit pathogens in temperate regions. Seven Colletotrichum isolates obtained from apple fruits with anthracnose symptoms, and two reference isolates C. gloeosporioides AVO 37 4B (avocado, Israel) and C. actuatum TUT 137 A (strawberry, Israel) were investigated. Pathogenicity of all nine isolates was demonstrated on wound-inoculated apple fruits by fulfilling Koch's postulates. Investigated morphological characteristics involved shape, colour and margin of the colony, as well as conidial shape. Observed ecological parameters were growth and sporulation of isolates after 4, 7 and 11 days of incubation at temperatures of 15, 20, 23, 25, 27, 30, 32 and 35 degrees C and after 10 days of incubation under conditions of day light regime and dark. The isolates were identified by PCR using species-specific primers. The data regarding growth were processed by factorial and one way ANOVA using software Statistica 10. All seven isolates obtained from apple fruits and reference C. acutatum isolate formed velvety, gray colonies with slightly wavy margin, and one celled fusiform conidia on PDA medium. Mycelium of the reference isolate C. gloeosporioides AVO 37 4B was snow-white and conidia were cylindrical. Maximum mycelial growth rate for isolates obtained from apple fruits was at temperature of 23 degrees C, for reference isolate C. acutatum at 27 degrees C and for C. gloeosporioides at 23-27 degrees C. Temperature of 35 degrees C proved to be restrictive for C. acutatum isolates. Isolate C. gloeosporioides had the fastest growth rate compared to all other isolates. Using PCR with species-specific primers all eight isolates were identified as C. acutatum (fragments sized 490 bp were amplified), and one as C. gloeosporioides (fragments sized 450 bp were amplified). The results suggest that differentiation between the two Colletotrichum species based on several morphological and ecological parameters is possible.",
publisher = "Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University",
journal = "Zemdirbyste",
title = "Morphological and ecological features as differentiation criteria for Colletotrichum species",
pages = "196-189",
number = "2",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1173"
}

Grahovac, M., Inđić, D., Vuković, S., Hrustić, J., Gvozdenac, S., Mihajlović, M.,& Tanović, B.. (2012). Morphological and ecological features as differentiation criteria for Colletotrichum species. in Zemdirbyste
Lithuanian Research Centre for Agriculture and Forestry; Vytautas Magnus University., 99(2), 189-196.
https://hdl.handle.net/21.15107/rcub_fiver_1173

Grahovac M, Inđić D, Vuković S, Hrustić J, Gvozdenac S, Mihajlović M, Tanović B. Morphological and ecological features as differentiation criteria for Colletotrichum species. in Zemdirbyste. 2012;99(2):189-196.
https://hdl.handle.net/21.15107/rcub_fiver_1173 .

Grahovac, Mila, Inđić, Dušanka, Vuković, Slavica, Hrustić, Jovana, Gvozdenac, Sonja, Mihajlović, Milica, Tanović, Brankica, "Morphological and ecological features as differentiation criteria for Colletotrichum species" in Zemdirbyste, 99, no. 2 (2012):189-196,
https://hdl.handle.net/21.15107/rcub_fiver_1173 .