TY  - JOUR
AU  - Vojvodić, Mira
AU  - Lazić, Dejan
AU  - Pešić, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2024
UR  - http://fiver.ifvcns.rs/handle/123456789/4557
AB  - Waitea circinata var. zeae, a pathogen with a relatively narrow host range, has recently been detected in cabbage and oilseed rape in Europe and worldwide. In this study, we developed specific conventional and real-time PCR protocols for direct detection of W. circinata var. zeae from mycelium and diseased plant tissue. The newly developed primer pair zeaefor1/zeaerew1, used in PCR protocols, specifically amplified only target isolates of W. circinata var. zeae when tested against isolates of 11 different binucleate and multinucleate anastomosis groups of Rhizoctonia spp. including AG-A, AG-G, AG-F, AG-U, AG-2-1, AG-2-2, AG-3, AG-4 HGI, AG-4 HGII, AG-4 HGIII, and AG-6 and common soil-borne pathogens. Total of nine previously published primer pairs designed for the detection of various Rhizoctonia spp. were also tested and did not amplify target isolates of W. circinata var. zeae. The detection limit of conventional and real-time PCR protocols was 10–2 and 10–5 (with starting concentration 9.5 ng/µl), respectively, and both methods are the first available tools for direct detection and identification of W. circinata var. zeae from mycelium and diseased oilseed rape seedlings. Both conventional and SYBR-Green-based real-time PCR protocols are cost-effective and provide a solid basis for further investigations of W. circinata var. zeae, particularly in relation to distribution, host range, and epidemiology.
PB  - Springer Nature
T2  - Journal of Plant Diseases and Protection
T1  - Specific detection of Waitea circinata var. zeae using conventional and real-time PCR
DO  - 10.1007/s41348-024-00926-w
ER  - 

@article{
author = "Vojvodić, Mira and Lazić, Dejan and Pešić, Brankica and Mitrović, Petar and Vico, Ivana and Bulajić, Aleksandra",
year = "2024",
abstract = "Waitea circinata var. zeae, a pathogen with a relatively narrow host range, has recently been detected in cabbage and oilseed rape in Europe and worldwide. In this study, we developed specific conventional and real-time PCR protocols for direct detection of W. circinata var. zeae from mycelium and diseased plant tissue. The newly developed primer pair zeaefor1/zeaerew1, used in PCR protocols, specifically amplified only target isolates of W. circinata var. zeae when tested against isolates of 11 different binucleate and multinucleate anastomosis groups of Rhizoctonia spp. including AG-A, AG-G, AG-F, AG-U, AG-2-1, AG-2-2, AG-3, AG-4 HGI, AG-4 HGII, AG-4 HGIII, and AG-6 and common soil-borne pathogens. Total of nine previously published primer pairs designed for the detection of various Rhizoctonia spp. were also tested and did not amplify target isolates of W. circinata var. zeae. The detection limit of conventional and real-time PCR protocols was 10–2 and 10–5 (with starting concentration 9.5 ng/µl), respectively, and both methods are the first available tools for direct detection and identification of W. circinata var. zeae from mycelium and diseased oilseed rape seedlings. Both conventional and SYBR-Green-based real-time PCR protocols are cost-effective and provide a solid basis for further investigations of W. circinata var. zeae, particularly in relation to distribution, host range, and epidemiology.",
publisher = "Springer Nature",
journal = "Journal of Plant Diseases and Protection",
title = "Specific detection of Waitea circinata var. zeae using conventional and real-time PCR",
doi = "10.1007/s41348-024-00926-w"
}

Vojvodić, M., Lazić, D., Pešić, B., Mitrović, P., Vico, I.,& Bulajić, A.. (2024). Specific detection of Waitea circinata var. zeae using conventional and real-time PCR. in Journal of Plant Diseases and Protection
Springer Nature..
https://doi.org/10.1007/s41348-024-00926-w

Vojvodić M, Lazić D, Pešić B, Mitrović P, Vico I, Bulajić A. Specific detection of Waitea circinata var. zeae using conventional and real-time PCR. in Journal of Plant Diseases and Protection. 2024;.
doi:10.1007/s41348-024-00926-w .

Vojvodić, Mira, Lazić, Dejan, Pešić, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, "Specific detection of Waitea circinata var. zeae using conventional and real-time PCR" in Journal of Plant Diseases and Protection (2024),
https://doi.org/10.1007/s41348-024-00926-w . .

TY  - CONF
AU  - Vojvodić, Mira
AU  - Tanović, Brankica
AU  - Mitrović, Petar
AU  - Vico, Ivana
AU  - Lazić, Dejan
AU  - Stevanović, Miloš
AU  - Bulajić, Aleksandra
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2373
AB  - Rhizoctonia spp. comprise soil-borne fungi widely distributed in temperate climate worldwide which are annually causing significant yield loses to a range of cultivated plants. A species status of Rhizoctonia spp. has not yet been established and the isolates are classified based on nuclei status as multinucleate and binucleate Rhizoctonia spp., and further divided into 13 anastomosis groups (AG) (AG-1-13) of multinucleate Rhizoctonia and 23 AG (AG-A-W) of binucleate Rhizoctonia. In this paper we are reporting morphological and molecular features of binucleate Rhizoctonia spp. AG-A causing black root rot on strawberry and multinucleate R. solani AG-2-2 causing root and crown rot of sugar beet. During 2015 and 2016 at several strawberry and sugar beet producing areas, plants expressing prominent root and crown necrosis and rot have been observed with disease incidence estimated at 20-30%.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
T1  - Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia
EP  - 32
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2373
ER  - 

@conference{
author = "Vojvodić, Mira and Tanović, Brankica and Mitrović, Petar and Vico, Ivana and Lazić, Dejan and Stevanović, Miloš and Bulajić, Aleksandra",
year = "2020",
abstract = "Rhizoctonia spp. comprise soil-borne fungi widely distributed in temperate climate worldwide which are annually causing significant yield loses to a range of cultivated plants. A species status of Rhizoctonia spp. has not yet been established and the isolates are classified based on nuclei status as multinucleate and binucleate Rhizoctonia spp., and further divided into 13 anastomosis groups (AG) (AG-1-13) of multinucleate Rhizoctonia and 23 AG (AG-A-W) of binucleate Rhizoctonia. In this paper we are reporting morphological and molecular features of binucleate Rhizoctonia spp. AG-A causing black root rot on strawberry and multinucleate R. solani AG-2-2 causing root and crown rot of sugar beet. During 2015 and 2016 at several strawberry and sugar beet producing areas, plants expressing prominent root and crown necrosis and rot have been observed with disease incidence estimated at 20-30%.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina",
title = "Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia",
pages = "32-31",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2373"
}

Vojvodić, M., Tanović, B., Mitrović, P., Vico, I., Lazić, D., Stevanović, M.,& Bulajić, A.. (2020). Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia. in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 31-32.
https://hdl.handle.net/21.15107/rcub_fiver_2373

Vojvodić M, Tanović B, Mitrović P, Vico I, Lazić D, Stevanović M, Bulajić A. Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia. in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina. 2020;:31-32.
https://hdl.handle.net/21.15107/rcub_fiver_2373 .

Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Lazić, Dejan, Stevanović, Miloš, Bulajić, Aleksandra, "Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia" in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina (2020):31-32,
https://hdl.handle.net/21.15107/rcub_fiver_2373 .

TY  - JOUR
AU  - Vojvodić, Mira
AU  - Lazić, Dejan
AU  - Mitrović, Petar
AU  - Tanović, Brankica
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1904
AB  - Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples.
AB  - Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet
T1  - Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji
EP  - 29
IS  - 1
SP  - 19
VL  - 34
DO  - 10.2298/PIF1901019V
ER  - 

@article{
author = "Vojvodić, Mira and Lazić, Dejan and Mitrović, Petar and Tanović, Brankica and Vico, Ivana and Bulajić, Aleksandra",
year = "2019",
abstract = "Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples., Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet, Konvencionalni i real-time PCR protokoli za detekciju i identifikaciju Rhizoctonia solani AG-2-2 prouzrokovača truleži korena šećerne repe u Srbiji",
pages = "29-19",
number = "1",
volume = "34",
doi = "10.2298/PIF1901019V"
}

Vojvodić, M., Lazić, D., Mitrović, P., Tanović, B., Vico, I.,& Bulajić, A.. (2019). Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(1), 19-29.
https://doi.org/10.2298/PIF1901019V

Vojvodić M, Lazić D, Mitrović P, Tanović B, Vico I, Bulajić A. Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet. in Pesticidi i fitomedicina. 2019;34(1):19-29.
doi:10.2298/PIF1901019V .

Vojvodić, Mira, Lazić, Dejan, Mitrović, Petar, Tanović, Brankica, Vico, Ivana, Bulajić, Aleksandra, "Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet" in Pesticidi i fitomedicina, 34, no. 1 (2019):19-29,
https://doi.org/10.2298/PIF1901019V . .