TY  - CONF
AU  - Mišković, Jovana
AU  - Rašeta, Milena
AU  - Tamindžić, Gordana
AU  - Krsmanović, Nenad
AU  - Karaman, Maja
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4162
AB  - Biopriming (BP) of seeds ensures increased resistance of seeds and young plants to stress, by integrating beneficial microorganisms and their products. In addition, research on filamentous fungi revealed their enormous potential in biotechnological processes and demonstrated their ability to increase plant
resistance to stressful conditions.
Aim:
The aim was to examine the influence of BPof pea seeds with polysaccharides (PSH) isolated from
submerged cultures of Schizophyllum commune originating from Serbia (SRB) and Italy (IT), on the antioxidant activity of the plant under drought.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - Abstract Book, 3rd Black Sea Association of Food Science and Technology Congress (B-FoST 2023), Belgrade, 13-14. December 2023
T1  - The effect of biopriming of pea seeds (Pisum sativum L.) with extracts of Schizophyllum commune Fr. 1815 on the antioxidant activity of the plant under stress conditions (drought)
EP  - 46
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4162
ER  - 

@conference{
author = "Mišković, Jovana and Rašeta, Milena and Tamindžić, Gordana and Krsmanović, Nenad and Karaman, Maja",
year = "2023",
abstract = "Biopriming (BP) of seeds ensures increased resistance of seeds and young plants to stress, by integrating beneficial microorganisms and their products. In addition, research on filamentous fungi revealed their enormous potential in biotechnological processes and demonstrated their ability to increase plant
resistance to stressful conditions.
Aim:
The aim was to examine the influence of BPof pea seeds with polysaccharides (PSH) isolated from
submerged cultures of Schizophyllum commune originating from Serbia (SRB) and Italy (IT), on the antioxidant activity of the plant under drought.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "Abstract Book, 3rd Black Sea Association of Food Science and Technology Congress (B-FoST 2023), Belgrade, 13-14. December 2023",
title = "The effect of biopriming of pea seeds (Pisum sativum L.) with extracts of Schizophyllum commune Fr. 1815 on the antioxidant activity of the plant under stress conditions (drought)",
pages = "46-46",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4162"
}

Mišković, J., Rašeta, M., Tamindžić, G., Krsmanović, N.,& Karaman, M.. (2023). The effect of biopriming of pea seeds (Pisum sativum L.) with extracts of Schizophyllum commune Fr. 1815 on the antioxidant activity of the plant under stress conditions (drought). in Abstract Book, 3rd Black Sea Association of Food Science and Technology Congress (B-FoST 2023), Belgrade, 13-14. December 2023
Belgrade : University of Belgrade, Faculty of Agriculture., 46-46.
https://hdl.handle.net/21.15107/rcub_fiver_4162

Mišković J, Rašeta M, Tamindžić G, Krsmanović N, Karaman M. The effect of biopriming of pea seeds (Pisum sativum L.) with extracts of Schizophyllum commune Fr. 1815 on the antioxidant activity of the plant under stress conditions (drought). in Abstract Book, 3rd Black Sea Association of Food Science and Technology Congress (B-FoST 2023), Belgrade, 13-14. December 2023. 2023;:46-46.
https://hdl.handle.net/21.15107/rcub_fiver_4162 .

Mišković, Jovana, Rašeta, Milena, Tamindžić, Gordana, Krsmanović, Nenad, Karaman, Maja, "The effect of biopriming of pea seeds (Pisum sativum L.) with extracts of Schizophyllum commune Fr. 1815 on the antioxidant activity of the plant under stress conditions (drought)" in Abstract Book, 3rd Black Sea Association of Food Science and Technology Congress (B-FoST 2023), Belgrade, 13-14. December 2023 (2023):46-46,
https://hdl.handle.net/21.15107/rcub_fiver_4162 .

TY  - CONF
AU  - Popov, Olivera
AU  - Leovac, Strahinja
AU  - Despotovski, Teodora
AU  - Karaman, Maja
AU  - Gadžurić, Slobodan
AU  - Pogorelc, Eva
AU  - Čapelja, Eleonora
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3213
AB  - Traditionally, mold control usually involves usage of highly toxic compounds, and this practice must be replaced with more environmentally friendly solutions (green chemistry). In this paper, antifungal activity of five newly synthesized ionic liquids (IL) was tested, in vitro. All ionic liquids are based on N-decyl- N, N, N-trimethylammonium chloride which differs only by a single substituent on a quaternary N- atom. The antifungal activity of IL was tested on Fusarium, Aspergillus, Alternaria, Trichoderma and Penicillium strains using the microdilution method by determining the minimal inhibitory and minimal fungicidal concentrations. Selected genera are major problem in food industry since they are the primary pathogens of agronomically important plants, as well as mycotoxin producers. All five IL used in this study showed antifungal effect in the range of 0.002 mol/dm3 to 0.036 mol/dm3. The greatest antifungal activity was observed when strains were treated with (C2OH)C10DMACI and (C2OOEt)C10DMACI IL. Results obtained in this study showed that all examined ILs have the potential to be used as effective antifungal agents.
PB  - Novi Sad : Matica srpska
C3  - Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.
T1  - Antifungal activity of surfactant ionic liquids on mycotoxigenic molds
EP  - 34
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3213
ER  - 

@conference{
author = "Popov, Olivera and Leovac, Strahinja and Despotovski, Teodora and Karaman, Maja and Gadžurić, Slobodan and Pogorelc, Eva and Čapelja, Eleonora",
year = "2022",
abstract = "Traditionally, mold control usually involves usage of highly toxic compounds, and this practice must be replaced with more environmentally friendly solutions (green chemistry). In this paper, antifungal activity of five newly synthesized ionic liquids (IL) was tested, in vitro. All ionic liquids are based on N-decyl- N, N, N-trimethylammonium chloride which differs only by a single substituent on a quaternary N- atom. The antifungal activity of IL was tested on Fusarium, Aspergillus, Alternaria, Trichoderma and Penicillium strains using the microdilution method by determining the minimal inhibitory and minimal fungicidal concentrations. Selected genera are major problem in food industry since they are the primary pathogens of agronomically important plants, as well as mycotoxin producers. All five IL used in this study showed antifungal effect in the range of 0.002 mol/dm3 to 0.036 mol/dm3. The greatest antifungal activity was observed when strains were treated with (C2OH)C10DMACI and (C2OOEt)C10DMACI IL. Results obtained in this study showed that all examined ILs have the potential to be used as effective antifungal agents.",
publisher = "Novi Sad : Matica srpska",
journal = "Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.",
title = "Antifungal activity of surfactant ionic liquids on mycotoxigenic molds",
pages = "34-34",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3213"
}

Popov, O., Leovac, S., Despotovski, T., Karaman, M., Gadžurić, S., Pogorelc, E.,& Čapelja, E.. (2022). Antifungal activity of surfactant ionic liquids on mycotoxigenic molds. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.
Novi Sad : Matica srpska., 34-34.
https://hdl.handle.net/21.15107/rcub_fiver_3213

Popov O, Leovac S, Despotovski T, Karaman M, Gadžurić S, Pogorelc E, Čapelja E. Antifungal activity of surfactant ionic liquids on mycotoxigenic molds. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.. 2022;:34-34.
https://hdl.handle.net/21.15107/rcub_fiver_3213 .

Popov, Olivera, Leovac, Strahinja, Despotovski, Teodora, Karaman, Maja, Gadžurić, Slobodan, Pogorelc, Eva, Čapelja, Eleonora, "Antifungal activity of surfactant ionic liquids on mycotoxigenic molds" in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022. (2022):34-34,
https://hdl.handle.net/21.15107/rcub_fiver_3213 .

TY  - CONF
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Miklič, Vladimir
AU  - Tamindžić, Gordana
AU  - Miljaković, Dragana
AU  - Karaman, Maja
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3212
AB  - The sunflower (Helianthus annuus L.) is one of the most important oil crops in the world and the most important cultivated oil crop in Serbia, where it is grown on about 160,000 to 210,000 ha and the seed yield ranging from 1.7 to 2.3 t/ha. Several seed-borne fungi including species of Alternaria, Aspergillus, Cladosporium, Drechslera, Epicoccum, Fusarium and Penicillium, have been reported from sunflower seeds and could cause different levels of losses in its production. During the routine seed quality control and seed health testing analysis of sunflower, according to ISTA Rules, Cladosporium spp. infection was observed on an average of 5%. The aim of this study was the isolation and identification of Cladosporium spp. based on their morphological characteristics and molecular analyses. Isolation of the pathogen was carried out by transferring infected seeds onto a potato dextrose agar (PDA), and incubated for 5 days at 25 °C. Cladosporium-like colonies were transferred onto fresh PDA and water agar (WA, 17 g agar and 1 liter of distilled H2O) to obtain monosporial isolates. Seven days later, five isolates formed grey-greyish brown, velvet-like colony with apically and laterally branched conidiophores. Margin of the colony was white to grey-olivaceous. Conidias were mostly globose to subglobose, 3-4.5 µm in diameter, mild to dark olivaceous brown.
PB  - Novi Sad : Matica srpska
C3  - Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.
T1  - Cladosporium cladosporoides, pathogen of sunflower seed
EP  - 79
SP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3212
ER  - 

@conference{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Miklič, Vladimir and Tamindžić, Gordana and Miljaković, Dragana and Karaman, Maja",
year = "2022",
abstract = "The sunflower (Helianthus annuus L.) is one of the most important oil crops in the world and the most important cultivated oil crop in Serbia, where it is grown on about 160,000 to 210,000 ha and the seed yield ranging from 1.7 to 2.3 t/ha. Several seed-borne fungi including species of Alternaria, Aspergillus, Cladosporium, Drechslera, Epicoccum, Fusarium and Penicillium, have been reported from sunflower seeds and could cause different levels of losses in its production. During the routine seed quality control and seed health testing analysis of sunflower, according to ISTA Rules, Cladosporium spp. infection was observed on an average of 5%. The aim of this study was the isolation and identification of Cladosporium spp. based on their morphological characteristics and molecular analyses. Isolation of the pathogen was carried out by transferring infected seeds onto a potato dextrose agar (PDA), and incubated for 5 days at 25 °C. Cladosporium-like colonies were transferred onto fresh PDA and water agar (WA, 17 g agar and 1 liter of distilled H2O) to obtain monosporial isolates. Seven days later, five isolates formed grey-greyish brown, velvet-like colony with apically and laterally branched conidiophores. Margin of the colony was white to grey-olivaceous. Conidias were mostly globose to subglobose, 3-4.5 µm in diameter, mild to dark olivaceous brown.",
publisher = "Novi Sad : Matica srpska",
journal = "Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.",
title = "Cladosporium cladosporoides, pathogen of sunflower seed",
pages = "79-78",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3212"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Miklič, V., Tamindžić, G., Miljaković, D.,& Karaman, M.. (2022). Cladosporium cladosporoides, pathogen of sunflower seed. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.
Novi Sad : Matica srpska., 78-79.
https://hdl.handle.net/21.15107/rcub_fiver_3212

Milošević D, Ignjatov M, Nikolić Z, Miklič V, Tamindžić G, Miljaković D, Karaman M. Cladosporium cladosporoides, pathogen of sunflower seed. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.. 2022;:78-79.
https://hdl.handle.net/21.15107/rcub_fiver_3212 .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Miklič, Vladimir, Tamindžić, Gordana, Miljaković, Dragana, Karaman, Maja, "Cladosporium cladosporoides, pathogen of sunflower seed" in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022. (2022):78-79,
https://hdl.handle.net/21.15107/rcub_fiver_3212 .

TY  - CONF
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Miklič, Vladimir
AU  - Karaman, Maja
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Dedić, Boško
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2910
AB  - Sunflower (Helianthus annuus L.) is one of the most important oilseed crops throughout the world, and in Serbia it is grown on about 160,000 to 210,000 ha. Alternaria alternata (Fr.) Keissler has been detected as the most frequent seed-borne pathogen, able to infect a wide variety of plant species in different countries. During a routine seed quality control and seed health testing analysis of sunflower seed in 2018-2019, according to ISTA Rules, Alternaria spp. infection was observed on an average of 18%. The aim of this study was the isolation and identification of Alternaria spp. based on their morphological characteristics and molecular analyses. Isolation of the pathogen was carried out by transferring infected seeds onto a potato dextrose agar (PDA), and subsequent incubation at 25°C. Seven days later, 21 isolates formed circular, smooth, grey to brownish-black colonies, with concentric zones of very intensive sporulation. Conidiophores were branched, solitary or in small groups, brown to olivaceous brown, ranging from 128 to 232 µm. All isolates were subcultured onto a PDA using a single spore technique.
PB  - Paris : International Sunflower Association
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
T1  - Colonization of sunflower seed with Alternaria alternata
EP  - 206
SP  - 206
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2910
ER  - 

@conference{
author = "Milošević, Dragana and Ignjatov, Maja and Miklič, Vladimir and Karaman, Maja and Nikolić, Zorica and Tamindžić, Gordana and Dedić, Boško",
year = "2022",
abstract = "Sunflower (Helianthus annuus L.) is one of the most important oilseed crops throughout the world, and in Serbia it is grown on about 160,000 to 210,000 ha. Alternaria alternata (Fr.) Keissler has been detected as the most frequent seed-borne pathogen, able to infect a wide variety of plant species in different countries. During a routine seed quality control and seed health testing analysis of sunflower seed in 2018-2019, according to ISTA Rules, Alternaria spp. infection was observed on an average of 18%. The aim of this study was the isolation and identification of Alternaria spp. based on their morphological characteristics and molecular analyses. Isolation of the pathogen was carried out by transferring infected seeds onto a potato dextrose agar (PDA), and subsequent incubation at 25°C. Seven days later, 21 isolates formed circular, smooth, grey to brownish-black colonies, with concentric zones of very intensive sporulation. Conidiophores were branched, solitary or in small groups, brown to olivaceous brown, ranging from 128 to 232 µm. All isolates were subcultured onto a PDA using a single spore technique.",
publisher = "Paris : International Sunflower Association, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia",
title = "Colonization of sunflower seed with Alternaria alternata",
pages = "206-206",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2910"
}

Milošević, D., Ignjatov, M., Miklič, V., Karaman, M., Nikolić, Z., Tamindžić, G.,& Dedić, B.. (2022). Colonization of sunflower seed with Alternaria alternata. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
Paris : International Sunflower Association., 206-206.
https://hdl.handle.net/21.15107/rcub_fiver_2910

Milošević D, Ignjatov M, Miklič V, Karaman M, Nikolić Z, Tamindžić G, Dedić B. Colonization of sunflower seed with Alternaria alternata. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia. 2022;:206-206.
https://hdl.handle.net/21.15107/rcub_fiver_2910 .

Milošević, Dragana, Ignjatov, Maja, Miklič, Vladimir, Karaman, Maja, Nikolić, Zorica, Tamindžić, Gordana, Dedić, Boško, "Colonization of sunflower seed with Alternaria alternata" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia (2022):206-206,
https://hdl.handle.net/21.15107/rcub_fiver_2910 .

TY  - JOUR
AU  - Karaman, Maja
AU  - Vranes, Milan
AU  - Tot, Aleksandar
AU  - Papović, Snezana
AU  - Miljaković, Dragana
AU  - Gadžurić, Slobodan
AU  - Ignjatov, Maja
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1968
AB  - The fungal genus Alternaria Nees 1816 includes the most prevalent pathogenic species that can cause crop diseases such as blight, black spot, and dark leaf spot. In accordance with the aim of developing modern sustainable approaches in agriculture for the replacement of synthetic and toxic substances with environmentally friendly alternatives, the objective of this study was to examine thein vitro antifungal activities of 18 newly synthesized ionic liquids (ILs) against three Alternaria strains: A. padwickii, A. dauci and A. linicola. The antifungal activities of the ILs were estimated via a microdilution method to establish minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values. The results confirmed that 17 of the 18 ILs showed strain specificity, including good antifungal activity toward Alternaria strains, with MIC and MFC values in the range of 0.04 to 0.43 mol dm(-3). The strongest antifungal effects toward all analyzed Alternaria strains were displayed by the compounds with long alkyl chains: [omim][Cl] (MIC/MFC: 0.042 mol dm(-3)), [dmim][Cl] (MIC/MFC: 0.043 mol dm(-3)), [ddmim][Cl] (MIC/MFC: 0.053 mol dm(-3)), [ddTSC][Br] (MIC/MFC: 0.053 mol dm(-3)), and [Allyl-mim][Cl] (MIC/MFC: 0.054 mol dm(-3)). The introduction of oxygen as a hydroxyl group resulted in less-pronounced toxicity towards Alternaria compared to the introduction of an ether group, while the contribution of the hydroxyl group was shown to be a more determining factor than the prolongation of the side-chain, resulting in overall fungicidal activity decrease. Our results indicate the possibility that the most effective ILs ([Allyl-mim][Cl], [omim][Cl], [dmim][Cl], [ddmim][Cl], [bTSC][Br], [hTSC][Br], [oTSC][Br], [dTSC][Br], and [ddTSC][Br]) could be applied to the control of plant diseases caused by Alternaria species, based on their potential as an environmentally friendly crop protection approach. Since salts based on TSC cations are significantly cheaper to synthesize, stable under mild conditions, and environmentally friendly after degradation, thiosemicarbazidium-based ILs can be a suitable replacement for commercially available imidazolium ILs.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Ionic liquids as potentially new antifungal agents against Alternaria species
EP  - 22323
IS  - 38
SP  - 22318
VL  - 10
DO  - 10.1039/d0ra02475a
ER  - 

@article{
author = "Karaman, Maja and Vranes, Milan and Tot, Aleksandar and Papović, Snezana and Miljaković, Dragana and Gadžurić, Slobodan and Ignjatov, Maja",
year = "2020",
abstract = "The fungal genus Alternaria Nees 1816 includes the most prevalent pathogenic species that can cause crop diseases such as blight, black spot, and dark leaf spot. In accordance with the aim of developing modern sustainable approaches in agriculture for the replacement of synthetic and toxic substances with environmentally friendly alternatives, the objective of this study was to examine thein vitro antifungal activities of 18 newly synthesized ionic liquids (ILs) against three Alternaria strains: A. padwickii, A. dauci and A. linicola. The antifungal activities of the ILs were estimated via a microdilution method to establish minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values. The results confirmed that 17 of the 18 ILs showed strain specificity, including good antifungal activity toward Alternaria strains, with MIC and MFC values in the range of 0.04 to 0.43 mol dm(-3). The strongest antifungal effects toward all analyzed Alternaria strains were displayed by the compounds with long alkyl chains: [omim][Cl] (MIC/MFC: 0.042 mol dm(-3)), [dmim][Cl] (MIC/MFC: 0.043 mol dm(-3)), [ddmim][Cl] (MIC/MFC: 0.053 mol dm(-3)), [ddTSC][Br] (MIC/MFC: 0.053 mol dm(-3)), and [Allyl-mim][Cl] (MIC/MFC: 0.054 mol dm(-3)). The introduction of oxygen as a hydroxyl group resulted in less-pronounced toxicity towards Alternaria compared to the introduction of an ether group, while the contribution of the hydroxyl group was shown to be a more determining factor than the prolongation of the side-chain, resulting in overall fungicidal activity decrease. Our results indicate the possibility that the most effective ILs ([Allyl-mim][Cl], [omim][Cl], [dmim][Cl], [ddmim][Cl], [bTSC][Br], [hTSC][Br], [oTSC][Br], [dTSC][Br], and [ddTSC][Br]) could be applied to the control of plant diseases caused by Alternaria species, based on their potential as an environmentally friendly crop protection approach. Since salts based on TSC cations are significantly cheaper to synthesize, stable under mild conditions, and environmentally friendly after degradation, thiosemicarbazidium-based ILs can be a suitable replacement for commercially available imidazolium ILs.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Ionic liquids as potentially new antifungal agents against Alternaria species",
pages = "22323-22318",
number = "38",
volume = "10",
doi = "10.1039/d0ra02475a"
}

Karaman, M., Vranes, M., Tot, A., Papović, S., Miljaković, D., Gadžurić, S.,& Ignjatov, M.. (2020). Ionic liquids as potentially new antifungal agents against Alternaria species. in RSC Advances
Royal Soc Chemistry, Cambridge., 10(38), 22318-22323.
https://doi.org/10.1039/d0ra02475a

Karaman M, Vranes M, Tot A, Papović S, Miljaković D, Gadžurić S, Ignjatov M. Ionic liquids as potentially new antifungal agents against Alternaria species. in RSC Advances. 2020;10(38):22318-22323.
doi:10.1039/d0ra02475a .

Karaman, Maja, Vranes, Milan, Tot, Aleksandar, Papović, Snezana, Miljaković, Dragana, Gadžurić, Slobodan, Ignjatov, Maja, "Ionic liquids as potentially new antifungal agents against Alternaria species" in RSC Advances, 10, no. 38 (2020):22318-22323,
https://doi.org/10.1039/d0ra02475a . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Karaman, Maja
AU  - Grahovac, Mila
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2066
AB  - Matthiola longipetala (Vent) DC, commonly known as "night-scented stock" or "evening stock" is the most widely cultivated species of the genus Matthiola in the family Brassicaceae. It is a common garden flower, available in a variety of colours, many of which are heavily scented and also used in floristry. An elevated incidence of Fusarium was observed during a routine quality control seed assay of M. longipetala obtained from a private production facility in Đurđevo, Serbia in 2018. To our knowledge, this is the first report of F. equiseti as a causal agent of seed rot on M. longipetala in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia
EP  - +
IS  - 9
SP  - 2516
VL  - 104
DO  - 10.1094/PDIS-03-20-0602-PDN
ER  - 

@article{
author = "Ivanović, Žarko and Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Karaman, Maja and Grahovac, Mila",
year = "2020",
abstract = "Matthiola longipetala (Vent) DC, commonly known as "night-scented stock" or "evening stock" is the most widely cultivated species of the genus Matthiola in the family Brassicaceae. It is a common garden flower, available in a variety of colours, many of which are heavily scented and also used in floristry. An elevated incidence of Fusarium was observed during a routine quality control seed assay of M. longipetala obtained from a private production facility in Đurđevo, Serbia in 2018. To our knowledge, this is the first report of F. equiseti as a causal agent of seed rot on M. longipetala in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia",
pages = "+-2516",
number = "9",
volume = "104",
doi = "10.1094/PDIS-03-20-0602-PDN"
}

Ivanović, Ž., Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Karaman, M.,& Grahovac, M.. (2020). First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 104(9), 2516-+.
https://doi.org/10.1094/PDIS-03-20-0602-PDN

Ivanović Ž, Milošević D, Ignjatov M, Marjanović-Jeromela A, Karaman M, Grahovac M. First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia. in Plant Disease. 2020;104(9):2516-+.
doi:10.1094/PDIS-03-20-0602-PDN .

Ivanović, Žarko, Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Karaman, Maja, Grahovac, Mila, "First Report of Fusarium equiseti as the Causal Agent of Seed Rot of Matthiola longipetala in Serbia" in Plant Disease, 104, no. 9 (2020):2516-+,
https://doi.org/10.1094/PDIS-03-20-0602-PDN . .

TY  - JOUR
AU  - Spremo, Nemanja
AU  - Bjelić, Dragana
AU  - Ignjatov, Maja
AU  - Karaman, Maja
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2533
AB  - Plant diseases caused by fungal pathogens can lead to major
economic losses in agriculture. Th e genus Alternaria includes
saprobic, endophytic and pathogenic species that may cause
various plant diseases such as blight disease. Symptoms of
Alternaria blight include the presence of irregular, oft en circular, brown to dark brown coloured leaf spots. Alternaria
spp. also cause collar rots, stem lesions, tuber and fruit rots
of their respective hosts. In addition, bacteria are one of the
most frequently biocontrol agents used to protect plants from
diseases. Bacillus species control disease through a variety of
mechanisms. Bacterial antagonists might act as inhibitors of
growth, development and reproduction of pathogen, or as inducers of host resistance in plant. Th e objective of this study
was to examine in vitro antifungal activity of ten antagonistic
Bacillus strains from collection of Department for Microbiology of Institute of Field and Vegetable Crops, Novi Sad. Strains
were originally isolated from the soil samples collected from
several localities of Vojvodina Province. Antifungal activity of
Bacillus strains against Alternaria padwickii, A. dauci and A.
linicola, obtained from rice, carrot and linseed, respectively
was tested using a dual plate assay. Th e results confi rmed that
all tested Bacillus strains showed antifungal acivity against Alternaria spp. Th e highest antagonistic activity was exhibited
by B. pumilus B11 (45.71% to 51.25%), B. subtilis B13 (45.71%
to 49.37%) and B. subtilis B32 (45.71% to 52.08%), while B.
pumilus B23 had the weakest antifungal activity. B. safensis B2
(35.24% to 48.33%), B. pumilus B21 (40.95% to 48.75%) and
B. pumilus B22 (33.50% to 49.17%) also demonstrated good
antifungal potential. A. dauci was the most sensitive fungus,
while the most resistant was A. linicola. Obtained results indicate the possibility of usage of the most eff ective Bacillus
strains as potential biocontrol agents of plant diseases caused
by Alternaria spp
PB  - Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)
T1  - Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp.
EP  - 169
SP  - 169
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2533
ER  - 

@article{
author = "Spremo, Nemanja and Bjelić, Dragana and Ignjatov, Maja and Karaman, Maja",
year = "2018",
abstract = "Plant diseases caused by fungal pathogens can lead to major
economic losses in agriculture. Th e genus Alternaria includes
saprobic, endophytic and pathogenic species that may cause
various plant diseases such as blight disease. Symptoms of
Alternaria blight include the presence of irregular, oft en circular, brown to dark brown coloured leaf spots. Alternaria
spp. also cause collar rots, stem lesions, tuber and fruit rots
of their respective hosts. In addition, bacteria are one of the
most frequently biocontrol agents used to protect plants from
diseases. Bacillus species control disease through a variety of
mechanisms. Bacterial antagonists might act as inhibitors of
growth, development and reproduction of pathogen, or as inducers of host resistance in plant. Th e objective of this study
was to examine in vitro antifungal activity of ten antagonistic
Bacillus strains from collection of Department for Microbiology of Institute of Field and Vegetable Crops, Novi Sad. Strains
were originally isolated from the soil samples collected from
several localities of Vojvodina Province. Antifungal activity of
Bacillus strains against Alternaria padwickii, A. dauci and A.
linicola, obtained from rice, carrot and linseed, respectively
was tested using a dual plate assay. Th e results confi rmed that
all tested Bacillus strains showed antifungal acivity against Alternaria spp. Th e highest antagonistic activity was exhibited
by B. pumilus B11 (45.71% to 51.25%), B. subtilis B13 (45.71%
to 49.37%) and B. subtilis B32 (45.71% to 52.08%), while B.
pumilus B23 had the weakest antifungal activity. B. safensis B2
(35.24% to 48.33%), B. pumilus B21 (40.95% to 48.75%) and
B. pumilus B22 (33.50% to 49.17%) also demonstrated good
antifungal potential. A. dauci was the most sensitive fungus,
while the most resistant was A. linicola. Obtained results indicate the possibility of usage of the most eff ective Bacillus
strains as potential biocontrol agents of plant diseases caused
by Alternaria spp",
publisher = "Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)",
title = "Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp.",
pages = "169-169",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2533"
}

Spremo, N., Bjelić, D., Ignjatov, M.,& Karaman, M.. (2018). Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp.. in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)
Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac., 42, 169-169.
https://hdl.handle.net/21.15107/rcub_fiver_2533

Spremo N, Bjelić D, Ignjatov M, Karaman M. Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp.. in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia). 2018;42:169-169.
https://hdl.handle.net/21.15107/rcub_fiver_2533 .

Spremo, Nemanja, Bjelić, Dragana, Ignjatov, Maja, Karaman, Maja, "Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp." in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia), 42 (2018):169-169,
https://hdl.handle.net/21.15107/rcub_fiver_2533 .

TY  - JOUR
AU  - Spremo, Nemanja
AU  - Bešir, Nataša
AU  - Vraneš, Milan
AU  - Gadžurić, Slobodan
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Karaman, Maja
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2534
AB  - A modern sustainable approach in agricultural practice would
be a replacement of synthetic and toxic substances in the
treatment of crop with benign and environmentally friendly
compounds, by using principles of green chemistry. One of
the possible directions could be using of ionic liquids (ILs),
salts with melting points below 100 °C, which are well-known
for manifesting antimicrobial activity. ILs can be used in the
development of novel sources of antimicrobial agents such as
antiseptics, biocides and antifungal agents. Furthermore, ILs
have already been reported as alternative ‘green’ solvents for a
wide range of reactions and technological processes. Considering the importance of research in the area of bioactivity of
ILs for discovery of new green ILs for diff erent purposes, the
aim of this study was to examine antifungal activity of nine
newly synthesized ILs against phytopathogenic Alternaria
strains isolated from from rice (A. padwickii), carrot (A. dauci) and linseed (A. linicola). Antifungal eff ect was estimated
by micro-plate microdilution method for establishing minimum inhibitory (MIC) and minimum fungicidal concentration (MFC). All ionic liquids showed strain specifi c but good
antifungal activity on Alternaria strains with MIC and MFC
detected at the range from 9.23 mg/ml to 75.89 mg/ml. Only
in case of the 1-(4-hydroxy-2-oxy)butyl -3-methylimidazolium chloride [OHC2OC2mIm][Cl], no antifungal eff ect on
A. dauci were observed. Comparing to A. padwickii and A.
dauci., A. linicola showed higher sensitivity to all tested ILs.
Th e obtained results indicate the possibility of usage of ILs
in biocontrol of plant diseases, representing their application
in crop protection. However, further research is necessary in
order to examine their toxicity and biodegradability in the environment.
PB  - Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)
T1  - Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains
EP  - 169
IS  - 42
SP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2534
ER  - 

@article{
author = "Spremo, Nemanja and Bešir, Nataša and Vraneš, Milan and Gadžurić, Slobodan and Ignjatov, Maja and Bjelić, Dragana and Karaman, Maja",
year = "2018",
abstract = "A modern sustainable approach in agricultural practice would
be a replacement of synthetic and toxic substances in the
treatment of crop with benign and environmentally friendly
compounds, by using principles of green chemistry. One of
the possible directions could be using of ionic liquids (ILs),
salts with melting points below 100 °C, which are well-known
for manifesting antimicrobial activity. ILs can be used in the
development of novel sources of antimicrobial agents such as
antiseptics, biocides and antifungal agents. Furthermore, ILs
have already been reported as alternative ‘green’ solvents for a
wide range of reactions and technological processes. Considering the importance of research in the area of bioactivity of
ILs for discovery of new green ILs for diff erent purposes, the
aim of this study was to examine antifungal activity of nine
newly synthesized ILs against phytopathogenic Alternaria
strains isolated from from rice (A. padwickii), carrot (A. dauci) and linseed (A. linicola). Antifungal eff ect was estimated
by micro-plate microdilution method for establishing minimum inhibitory (MIC) and minimum fungicidal concentration (MFC). All ionic liquids showed strain specifi c but good
antifungal activity on Alternaria strains with MIC and MFC
detected at the range from 9.23 mg/ml to 75.89 mg/ml. Only
in case of the 1-(4-hydroxy-2-oxy)butyl -3-methylimidazolium chloride [OHC2OC2mIm][Cl], no antifungal eff ect on
A. dauci were observed. Comparing to A. padwickii and A.
dauci., A. linicola showed higher sensitivity to all tested ILs.
Th e obtained results indicate the possibility of usage of ILs
in biocontrol of plant diseases, representing their application
in crop protection. However, further research is necessary in
order to examine their toxicity and biodegradability in the environment.",
publisher = "Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)",
title = "Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains",
pages = "169-169",
number = "42",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2534"
}

Spremo, N., Bešir, N., Vraneš, M., Gadžurić, S., Ignjatov, M., Bjelić, D.,& Karaman, M.. (2018). Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains. in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)
Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac.(42), 169-169.
https://hdl.handle.net/21.15107/rcub_fiver_2534

Spremo N, Bešir N, Vraneš M, Gadžurić S, Ignjatov M, Bjelić D, Karaman M. Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains. in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia). 2018;(42):169-169.
https://hdl.handle.net/21.15107/rcub_fiver_2534 .

Spremo, Nemanja, Bešir, Nataša, Vraneš, Milan, Gadžurić, Slobodan, Ignjatov, Maja, Bjelić, Dragana, Karaman, Maja, "Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains" in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia), no. 42 (2018):169-169,
https://hdl.handle.net/21.15107/rcub_fiver_2534 .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Ivanović, Žarko
AU  - Karaman, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1762
AB  - During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank.
AB  - Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia
T1  - Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji
EP  - 129
IS  - 3
SP  - 125
VL  - 55
DO  - 10.5937/RatPov1803125I
ER  - 

@article{
author = "Ignjatov, Maja and Milošević, Dragana and Ivanović, Žarko and Karaman, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica",
year = "2018",
abstract = "During the past few years, symptoms of garlic clove rot have appeared in storage facilities at different localities in Serbia. Thirteen fungal isolates were isolated from samples of decayed garlic cloves (Allium sativum L.). Based on the colony and fungal morphology, isolated fungi were tentatively identified as F. proliferatum. Pathogenicity of 13 F. proliferatum strains were confirmed by inoculating garlic cloves of Allium sativum (spring cv. Labud, Sedef; winter cv. Bosut, Ranko) and Allium ampeloprasum (cv. Biser). Isolate JBL532 was classified as the most aggressive, while isolate B3 was the least pathogenic. To confirm morphological identification of 13 Fusarium isolates, amplification of TEF-1α gene was performed using polymerase chain reaction (PCR) with the primer pair: EF1 and EF2. All isolates were cryopreserved at -80°C and deposited in the NCBI GenBank., Tokom proteklih godina u skladištima na različitim lokalitetima u Srbiji pojavili su se simptomi truleži i popadanja belog luka (Allium sativum L.). Izolacijom je dobijeno 13 izolata gljiva roda Fusarium koji su na osnovu morfoloških karakteristika identifikovani kao F. proliferatum. Patogenost izolata je potvrđena inokulacijom različitih genotipova belog luka Allium sativum (prolećne sorte Labud, Sedef, jesenje sorte Bosut, Ranko) i Allium ampeloprasum (sorta Biser). Na osnovu ocene patogenosti, izolat JBL532 se izdvojio kao najagresivniji, dok se izolat B3 pokazao kao najmanje patogen. Morfološka identifikacija potvrđena je amplifikacijom TEF-1α gena, korišćenjem prajmera EF1 i EF2. Svi izolati se čuvaju na -80°C i deponovani su u NCBI bazu gde su im dodeljeni jedinstveni pristupni brojevi.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia, Morfološke i patogene odlike izolata Fusarium proliferatum prouzrokovača truleži belog luka (Allium sativum L.) - u Srbiji",
pages = "129-125",
number = "3",
volume = "55",
doi = "10.5937/RatPov1803125I"
}

Ignjatov, M., Milošević, D., Ivanović, Ž., Karaman, M., Vlajić, S., Nikolić, Z.,& Gvozdanović-Varga, J.. (2018). Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 55(3), 125-129.
https://doi.org/10.5937/RatPov1803125I

Ignjatov M, Milošević D, Ivanović Ž, Karaman M, Vlajić S, Nikolić Z, Gvozdanović-Varga J. Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2018;55(3):125-129.
doi:10.5937/RatPov1803125I .

Ignjatov, Maja, Milošević, Dragana, Ivanović, Žarko, Karaman, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, "Morphological and pathogenic properties of Fusarium proliferatum isolates: The causal agent of garlic (Allium sativum L.): Rot in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 55, no. 3 (2018):125-129,
https://doi.org/10.5937/RatPov1803125I . .

TY  - JOUR
AU  - Bjelić, Dragana
AU  - Ignjatov, Maja
AU  - Marinković, Jelena
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Karaman, Maja
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1745
AB  - Clove rot caused by Fusarium spp. is a very important disease of common garlic (Allium sativum L.) occuring in many areas of the world. However, there is a lack of data about biocontrol of these pathogens. Bacillus species are attractive for research due to their potential use in the biological control of fungal diseases. The aim of this study was to select effective biocontrol agents from a series of indigenous Bacillus spp. isolated from soil. Bacterial isolates positive for hydrolytic enzymes production were screened for antifungal activity against Fusarium spp. isolated from infected garlic cloves. Polymerase chain reaction (PCR) analyses were used for molecular identification of bacterial (16S rDNA gene) and fungal (EF-1 alpha gene) isolates, and detection of biosynthetic genes for antimicrobial lipopeptides (surfactin, iturin, bacillomycin D and fengycin) in Bacillus spp. The obtained results confirmed the presence of Fusarium tricinctum, F. oxysporum f. sp. cepae, F. proliferatum, F. acuminatum and F. verticillioides as the causal agents of garlic clove rot. Four bacterial isolates identified as Bacillus subtilis exhibited the highest antagonistic effect during in vitro testing of antifungal activity (up to 71% reduction in fungal growth), and caused a significant suppression of garlic clove infection (up to 58% reduction in rot symptoms) in situ. Analysis of the antifungal compounds involved in the antagonistic activity of the examined isolates revealed their ability to produce the antibiotic lipopeptide surfactin. The most effective isolates of B. subtilis could be used as potential biocontrol agents of garlic clove rot.
PB  - Lithuanian Research Centre Agriculture & Forestry, Kedainiu R
T2  - Zemdirbyste-Agriculture
T1  - Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic
EP  - 376
IS  - 4
SP  - 369
VL  - 105
DO  - 10.13080/z-a.2018.105.047
ER  - 

@article{
author = "Bjelić, Dragana and Ignjatov, Maja and Marinković, Jelena and Milošević, Dragana and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Karaman, Maja",
year = "2018",
abstract = "Clove rot caused by Fusarium spp. is a very important disease of common garlic (Allium sativum L.) occuring in many areas of the world. However, there is a lack of data about biocontrol of these pathogens. Bacillus species are attractive for research due to their potential use in the biological control of fungal diseases. The aim of this study was to select effective biocontrol agents from a series of indigenous Bacillus spp. isolated from soil. Bacterial isolates positive for hydrolytic enzymes production were screened for antifungal activity against Fusarium spp. isolated from infected garlic cloves. Polymerase chain reaction (PCR) analyses were used for molecular identification of bacterial (16S rDNA gene) and fungal (EF-1 alpha gene) isolates, and detection of biosynthetic genes for antimicrobial lipopeptides (surfactin, iturin, bacillomycin D and fengycin) in Bacillus spp. The obtained results confirmed the presence of Fusarium tricinctum, F. oxysporum f. sp. cepae, F. proliferatum, F. acuminatum and F. verticillioides as the causal agents of garlic clove rot. Four bacterial isolates identified as Bacillus subtilis exhibited the highest antagonistic effect during in vitro testing of antifungal activity (up to 71% reduction in fungal growth), and caused a significant suppression of garlic clove infection (up to 58% reduction in rot symptoms) in situ. Analysis of the antifungal compounds involved in the antagonistic activity of the examined isolates revealed their ability to produce the antibiotic lipopeptide surfactin. The most effective isolates of B. subtilis could be used as potential biocontrol agents of garlic clove rot.",
publisher = "Lithuanian Research Centre Agriculture & Forestry, Kedainiu R",
journal = "Zemdirbyste-Agriculture",
title = "Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic",
pages = "376-369",
number = "4",
volume = "105",
doi = "10.13080/z-a.2018.105.047"
}

Bjelić, D., Ignjatov, M., Marinković, J., Milošević, D., Nikolić, Z., Gvozdanović-Varga, J.,& Karaman, M.. (2018). Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic. in Zemdirbyste-Agriculture
Lithuanian Research Centre Agriculture & Forestry, Kedainiu R., 105(4), 369-376.
https://doi.org/10.13080/z-a.2018.105.047

Bjelić D, Ignjatov M, Marinković J, Milošević D, Nikolić Z, Gvozdanović-Varga J, Karaman M. Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic. in Zemdirbyste-Agriculture. 2018;105(4):369-376.
doi:10.13080/z-a.2018.105.047 .

Bjelić, Dragana, Ignjatov, Maja, Marinković, Jelena, Milošević, Dragana, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Karaman, Maja, "Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic" in Zemdirbyste-Agriculture, 105, no. 4 (2018):369-376,
https://doi.org/10.13080/z-a.2018.105.047 . .

TY  - JOUR
AU  - Bjelić, Dragana
AU  - Ignjatov, Maja
AU  - Marinković, Jelena
AU  - Spremo, Nemanja R.
AU  - Karaman, Maja
AU  - Nikolić, Zorica
AU  - Ivanović, Žarko
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1650
AB  - Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.
AB  - Biokontrola fitopatogena predstavlja alternativu primeni pesticida, s obzirom da bakterije označene terminom PGPR (Plant Growth Promoting Rhizobacteria) stimulišu biljni rast i štite biljke od bolesti. Zahvaljujući sposobnosti da formiraju veoma rezistentne endospore i produkuju širok spektar antimikrobnih supstanci, vrste roda Bacillus su veoma zastupljene u zemljištu i pogodne za primenu u biotehnologiji. Cilj istraživanja bio je da se utvrdi antifungalna aktivnost jedanaest Bacillus spp. izolata iz zemljišta s različitih lokaliteta u Vojvodini. Sposobnost bakterijskih izolata da inhibiraju rast pet izolata gljiva ispitana je metodom dvojne kultivacije. Izolati Bacillus spp. ispoljili su najveću antifungalnu aktivnost prema Fusarium proliferatum, Fusarium oxysporum f. sp. cepae, i Alternaria padwickii, dok je najmanji antagonistički efekat utvrđen prema Fusarium verticillioides i Fusarium graminearum. Efektivni izolati identifikovani su kao Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) i Bacillus megaterium (B8, B9). Najveću antifungalnu aktivnost ispoljili su izolati B. subtilis B5 (39-62%) i B7 (40-71%). Ovi izolati mogu se koristiti kao potencijalni agensi za biološku kontrolu biljnih bolesti.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antifungal activity of indigenous Bacillus spp. isolated from soil
T1  - Antifungalna aktivnost prirodnih Bacillus spp. izolata iz zemljišta
EP  - 269
IS  - 133
SP  - 261
DO  - 10.2298/ZMSPN1733261B
ER  - 

@article{
author = "Bjelić, Dragana and Ignjatov, Maja and Marinković, Jelena and Spremo, Nemanja R. and Karaman, Maja and Nikolić, Zorica and Ivanović, Žarko",
year = "2017",
abstract = "Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases., Biokontrola fitopatogena predstavlja alternativu primeni pesticida, s obzirom da bakterije označene terminom PGPR (Plant Growth Promoting Rhizobacteria) stimulišu biljni rast i štite biljke od bolesti. Zahvaljujući sposobnosti da formiraju veoma rezistentne endospore i produkuju širok spektar antimikrobnih supstanci, vrste roda Bacillus su veoma zastupljene u zemljištu i pogodne za primenu u biotehnologiji. Cilj istraživanja bio je da se utvrdi antifungalna aktivnost jedanaest Bacillus spp. izolata iz zemljišta s različitih lokaliteta u Vojvodini. Sposobnost bakterijskih izolata da inhibiraju rast pet izolata gljiva ispitana je metodom dvojne kultivacije. Izolati Bacillus spp. ispoljili su najveću antifungalnu aktivnost prema Fusarium proliferatum, Fusarium oxysporum f. sp. cepae, i Alternaria padwickii, dok je najmanji antagonistički efekat utvrđen prema Fusarium verticillioides i Fusarium graminearum. Efektivni izolati identifikovani su kao Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) i Bacillus megaterium (B8, B9). Najveću antifungalnu aktivnost ispoljili su izolati B. subtilis B5 (39-62%) i B7 (40-71%). Ovi izolati mogu se koristiti kao potencijalni agensi za biološku kontrolu biljnih bolesti.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of indigenous Bacillus spp. isolated from soil, Antifungalna aktivnost prirodnih Bacillus spp. izolata iz zemljišta",
pages = "269-261",
number = "133",
doi = "10.2298/ZMSPN1733261B"
}

Bjelić, D., Ignjatov, M., Marinković, J., Spremo, N. R., Karaman, M., Nikolić, Z.,& Ivanović, Ž.. (2017). Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(133), 261-269.
https://doi.org/10.2298/ZMSPN1733261B

Bjelić D, Ignjatov M, Marinković J, Spremo NR, Karaman M, Nikolić Z, Ivanović Ž. Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke. 2017;(133):261-269.
doi:10.2298/ZMSPN1733261B .

Bjelić, Dragana, Ignjatov, Maja, Marinković, Jelena, Spremo, Nemanja R., Karaman, Maja, Nikolić, Zorica, Ivanović, Žarko, "Antifungal activity of indigenous Bacillus spp. isolated from soil" in Zbornik Matice srpske za prirodne nauke, no. 133 (2017):261-269,
https://doi.org/10.2298/ZMSPN1733261B . .

TY  - JOUR
AU  - Spremo, Nemanja R.
AU  - Tesanović, Kristina D.
AU  - Rakić, Milana S.
AU  - Janjušević, Ljiljana N.
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Karaman, Maja
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1649
AB  - During the last decades, intensive application of synthetic fungicides in the agricultural crop protection practice caused growing concern for the existence of toxic chemical residues in food as well as in the whole environment. Instead of using synthetic fungicides, it is suggested that crop protection be carried out by using preparations based on compounds of natural origin (secondary metabolites of plants or microorganisms, including macrofungi from Basidiomycota) as biological control agents. The potential of macrofungal species as biocontrol agents was analyzed in this investigation of eight autochthonous species from different locations in Serbia. Both the terricolous species: Coprinus comatus, Coprinellus truncorum, Amanita strobiliformis, Hydnum repandum and the lignicolous species: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor and Bjerkandera adusta were examined, with an aim to detect some novel sources of antifungal agents. This study surveyed antifungal activity of selected macrofungal extracts (MeOH, EtOH and CHCl3) against phytopathogenic Fusarium and Alternaria strains isolated from garlic, soybean and rice: F. proliferatum, F. verticillioides, F. proliferatum, F. graminearum and A. padwickii. Microdilution method in 96 well micro-plates was applied for the estimation of antifungal effects of macrofungi extracts in the range from 24.75 to 198.00 mg/ml and determination of minimal inhibitory (MIC) and minimal fungicidal concentration (MFC). EtOH extract of mychorhizal species H. repandum showed antifungal activity against all analyzed phytopathogenic strains, with the strongest effect on Fusarium strains (MIC 24.75 mg/ml; MFC 24.75 mg/ml). Among others, MeOH extracts of S. subtomentosum and C. micaceus showed similar effects while only B. adusta showed slight effect on Fusarium strains (MIC 24.75-99.00 mg/ml; MFC 24.75-99.00 mg/ml) and none effect on A. padwickii. The obtained results indicate the possibility of using examined extracts as efficient antifungal agents and provide the basis for the new formulations of biocontrol agents against phytopathogenic fungi in the future.
AB  - Tokom poslednjih decenija intezivna primena sintetičkih fungicida u zaštiti poljoprivrednih useva izaziva sve veću zabrinutost ljudi zbog prisustva toksičnih hemijskih ostataka u prehrambenim proizvodima kao i celoj okolini. Umesto korišćenja sintetičkih fungicida, preporučuje se da se zaštita useva vrši korišćenjem preparata na bazi jedinjenja prirodnog porekla (sekundarni metaboliti biljaka ili mikroorganizama uključujući i makrogljive iz razdela Basidiomycota) kao agense biološke kontrole. U ovom radu istraživan je potencijal agenasa biološke kontrole za osam autohtonih vrsta makrogljiva s različitih lokaliteta u Srbiji. Obe, terikolne vrste: Coprinus comatus, Coprinellus truncorum, Amanita strobiliformis, Hydnum repandum i lignikolne vrste: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor i Bjerkandera adusta su istražene s ciljem da se otkriju neki novi izvori antifungalnih agenasa. U okviru ovog rada istražena je antifungalna aktivnost odabranih ekstrakata makrogljiva (metanolni, etanolni i hloroformski) protiv fitopatogenih sojeva Fusarium i Alternaria izolovanih s belog luka, soje i pirinča: F. proliferatum, F. verticillioides, F. proliferatum, F. graminearum i A. padwickii. Za procenu antifungalnog efekta ekstrakata makrogljiva upotrebljena je mikrodiluciona metoda mikrotitar pločama s 96 velova u opsegu koncentracija od 24,75 do 198,00 mg/ml i determinaciju minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije (MFC). Etanolni ekstrakt mikorizne vrste H. repandum pokazao je atifungalnu aktivnost prema svim analiziranim fitopatogenim sojevima, sa najjačim efektom prema sojevima Fusarium (MIC 24,75 mg/ml; MFC 24,75 mg/ml). Između ostalog, sličan efekat pokazali su i metanolni ekstrakti S. subtomentosum i C. micaceus, dok je samo B. adusta imala blagi efekat na sojeve Fusarium (MIC 24,75-99,00 mg/ml; MFC 24,75-99,00 mg/ml), ali ne i na A. padwickii. Dobijeni rezultati ukazuju na mogućnost korišćenja ispitanih ekstrakata kao veoma efikasnih antifungalnih agenasa i samim tim oni predstavljaju osnovu za nove formulacije biokontrolnih agenasa protiv fitopatogenih gljiva u budućnosti.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp.
T1  - Antifungalna aktivnost ekstrakata makrogljiva na fitopatogene sojeve gljiva rodova Fusarium sp. i Alternaria sp.
EP  - 240
IS  - 133
SP  - 231
DO  - 10.2298/ZMSPN1733231S
ER  - 

@article{
author = "Spremo, Nemanja R. and Tesanović, Kristina D. and Rakić, Milana S. and Janjušević, Ljiljana N. and Ignjatov, Maja and Bjelić, Dragana and Karaman, Maja",
year = "2017",
abstract = "During the last decades, intensive application of synthetic fungicides in the agricultural crop protection practice caused growing concern for the existence of toxic chemical residues in food as well as in the whole environment. Instead of using synthetic fungicides, it is suggested that crop protection be carried out by using preparations based on compounds of natural origin (secondary metabolites of plants or microorganisms, including macrofungi from Basidiomycota) as biological control agents. The potential of macrofungal species as biocontrol agents was analyzed in this investigation of eight autochthonous species from different locations in Serbia. Both the terricolous species: Coprinus comatus, Coprinellus truncorum, Amanita strobiliformis, Hydnum repandum and the lignicolous species: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor and Bjerkandera adusta were examined, with an aim to detect some novel sources of antifungal agents. This study surveyed antifungal activity of selected macrofungal extracts (MeOH, EtOH and CHCl3) against phytopathogenic Fusarium and Alternaria strains isolated from garlic, soybean and rice: F. proliferatum, F. verticillioides, F. proliferatum, F. graminearum and A. padwickii. Microdilution method in 96 well micro-plates was applied for the estimation of antifungal effects of macrofungi extracts in the range from 24.75 to 198.00 mg/ml and determination of minimal inhibitory (MIC) and minimal fungicidal concentration (MFC). EtOH extract of mychorhizal species H. repandum showed antifungal activity against all analyzed phytopathogenic strains, with the strongest effect on Fusarium strains (MIC 24.75 mg/ml; MFC 24.75 mg/ml). Among others, MeOH extracts of S. subtomentosum and C. micaceus showed similar effects while only B. adusta showed slight effect on Fusarium strains (MIC 24.75-99.00 mg/ml; MFC 24.75-99.00 mg/ml) and none effect on A. padwickii. The obtained results indicate the possibility of using examined extracts as efficient antifungal agents and provide the basis for the new formulations of biocontrol agents against phytopathogenic fungi in the future., Tokom poslednjih decenija intezivna primena sintetičkih fungicida u zaštiti poljoprivrednih useva izaziva sve veću zabrinutost ljudi zbog prisustva toksičnih hemijskih ostataka u prehrambenim proizvodima kao i celoj okolini. Umesto korišćenja sintetičkih fungicida, preporučuje se da se zaštita useva vrši korišćenjem preparata na bazi jedinjenja prirodnog porekla (sekundarni metaboliti biljaka ili mikroorganizama uključujući i makrogljive iz razdela Basidiomycota) kao agense biološke kontrole. U ovom radu istraživan je potencijal agenasa biološke kontrole za osam autohtonih vrsta makrogljiva s različitih lokaliteta u Srbiji. Obe, terikolne vrste: Coprinus comatus, Coprinellus truncorum, Amanita strobiliformis, Hydnum repandum i lignikolne vrste: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor i Bjerkandera adusta su istražene s ciljem da se otkriju neki novi izvori antifungalnih agenasa. U okviru ovog rada istražena je antifungalna aktivnost odabranih ekstrakata makrogljiva (metanolni, etanolni i hloroformski) protiv fitopatogenih sojeva Fusarium i Alternaria izolovanih s belog luka, soje i pirinča: F. proliferatum, F. verticillioides, F. proliferatum, F. graminearum i A. padwickii. Za procenu antifungalnog efekta ekstrakata makrogljiva upotrebljena je mikrodiluciona metoda mikrotitar pločama s 96 velova u opsegu koncentracija od 24,75 do 198,00 mg/ml i determinaciju minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije (MFC). Etanolni ekstrakt mikorizne vrste H. repandum pokazao je atifungalnu aktivnost prema svim analiziranim fitopatogenim sojevima, sa najjačim efektom prema sojevima Fusarium (MIC 24,75 mg/ml; MFC 24,75 mg/ml). Između ostalog, sličan efekat pokazali su i metanolni ekstrakti S. subtomentosum i C. micaceus, dok je samo B. adusta imala blagi efekat na sojeve Fusarium (MIC 24,75-99,00 mg/ml; MFC 24,75-99,00 mg/ml), ali ne i na A. padwickii. Dobijeni rezultati ukazuju na mogućnost korišćenja ispitanih ekstrakata kao veoma efikasnih antifungalnih agenasa i samim tim oni predstavljaju osnovu za nove formulacije biokontrolnih agenasa protiv fitopatogenih gljiva u budućnosti.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp., Antifungalna aktivnost ekstrakata makrogljiva na fitopatogene sojeve gljiva rodova Fusarium sp. i Alternaria sp.",
pages = "240-231",
number = "133",
doi = "10.2298/ZMSPN1733231S"
}

Spremo, N. R., Tesanović, K. D., Rakić, M. S., Janjušević, L. N., Ignjatov, M., Bjelić, D.,& Karaman, M.. (2017). Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp.. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(133), 231-240.
https://doi.org/10.2298/ZMSPN1733231S

Spremo NR, Tesanović KD, Rakić MS, Janjušević LN, Ignjatov M, Bjelić D, Karaman M. Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp.. in Zbornik Matice srpske za prirodne nauke. 2017;(133):231-240.
doi:10.2298/ZMSPN1733231S .

Spremo, Nemanja R., Tesanović, Kristina D., Rakić, Milana S., Janjušević, Ljiljana N., Ignjatov, Maja, Bjelić, Dragana, Karaman, Maja, "Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp." in Zbornik Matice srpske za prirodne nauke, no. 133 (2017):231-240,
https://doi.org/10.2298/ZMSPN1733231S . .