TY  - JOUR
AU  - Spremo, Nemanja
AU  - Bjelić, Dragana
AU  - Ignjatov, Maja
AU  - Karaman, Maja
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2533
AB  - Plant diseases caused by fungal pathogens can lead to major
economic losses in agriculture. Th e genus Alternaria includes
saprobic, endophytic and pathogenic species that may cause
various plant diseases such as blight disease. Symptoms of
Alternaria blight include the presence of irregular, oft en circular, brown to dark brown coloured leaf spots. Alternaria
spp. also cause collar rots, stem lesions, tuber and fruit rots
of their respective hosts. In addition, bacteria are one of the
most frequently biocontrol agents used to protect plants from
diseases. Bacillus species control disease through a variety of
mechanisms. Bacterial antagonists might act as inhibitors of
growth, development and reproduction of pathogen, or as inducers of host resistance in plant. Th e objective of this study
was to examine in vitro antifungal activity of ten antagonistic
Bacillus strains from collection of Department for Microbiology of Institute of Field and Vegetable Crops, Novi Sad. Strains
were originally isolated from the soil samples collected from
several localities of Vojvodina Province. Antifungal activity of
Bacillus strains against Alternaria padwickii, A. dauci and A.
linicola, obtained from rice, carrot and linseed, respectively
was tested using a dual plate assay. Th e results confi rmed that
all tested Bacillus strains showed antifungal acivity against Alternaria spp. Th e highest antagonistic activity was exhibited
by B. pumilus B11 (45.71% to 51.25%), B. subtilis B13 (45.71%
to 49.37%) and B. subtilis B32 (45.71% to 52.08%), while B.
pumilus B23 had the weakest antifungal activity. B. safensis B2
(35.24% to 48.33%), B. pumilus B21 (40.95% to 48.75%) and
B. pumilus B22 (33.50% to 49.17%) also demonstrated good
antifungal potential. A. dauci was the most sensitive fungus,
while the most resistant was A. linicola. Obtained results indicate the possibility of usage of the most eff ective Bacillus
strains as potential biocontrol agents of plant diseases caused
by Alternaria spp
PB  - Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)
T1  - Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp.
EP  - 169
SP  - 169
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2533
ER  - 

@article{
author = "Spremo, Nemanja and Bjelić, Dragana and Ignjatov, Maja and Karaman, Maja",
year = "2018",
abstract = "Plant diseases caused by fungal pathogens can lead to major
economic losses in agriculture. Th e genus Alternaria includes
saprobic, endophytic and pathogenic species that may cause
various plant diseases such as blight disease. Symptoms of
Alternaria blight include the presence of irregular, oft en circular, brown to dark brown coloured leaf spots. Alternaria
spp. also cause collar rots, stem lesions, tuber and fruit rots
of their respective hosts. In addition, bacteria are one of the
most frequently biocontrol agents used to protect plants from
diseases. Bacillus species control disease through a variety of
mechanisms. Bacterial antagonists might act as inhibitors of
growth, development and reproduction of pathogen, or as inducers of host resistance in plant. Th e objective of this study
was to examine in vitro antifungal activity of ten antagonistic
Bacillus strains from collection of Department for Microbiology of Institute of Field and Vegetable Crops, Novi Sad. Strains
were originally isolated from the soil samples collected from
several localities of Vojvodina Province. Antifungal activity of
Bacillus strains against Alternaria padwickii, A. dauci and A.
linicola, obtained from rice, carrot and linseed, respectively
was tested using a dual plate assay. Th e results confi rmed that
all tested Bacillus strains showed antifungal acivity against Alternaria spp. Th e highest antagonistic activity was exhibited
by B. pumilus B11 (45.71% to 51.25%), B. subtilis B13 (45.71%
to 49.37%) and B. subtilis B32 (45.71% to 52.08%), while B.
pumilus B23 had the weakest antifungal activity. B. safensis B2
(35.24% to 48.33%), B. pumilus B21 (40.95% to 48.75%) and
B. pumilus B22 (33.50% to 49.17%) also demonstrated good
antifungal potential. A. dauci was the most sensitive fungus,
while the most resistant was A. linicola. Obtained results indicate the possibility of usage of the most eff ective Bacillus
strains as potential biocontrol agents of plant diseases caused
by Alternaria spp",
publisher = "Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)",
title = "Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp.",
pages = "169-169",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2533"
}

Spremo, N., Bjelić, D., Ignjatov, M.,& Karaman, M.. (2018). Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp.. in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)
Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac., 42, 169-169.
https://hdl.handle.net/21.15107/rcub_fiver_2533

Spremo N, Bjelić D, Ignjatov M, Karaman M. Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp.. in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia). 2018;42:169-169.
https://hdl.handle.net/21.15107/rcub_fiver_2533 .

Spremo, Nemanja, Bjelić, Dragana, Ignjatov, Maja, Karaman, Maja, "Bacillus strains as potential agents for the biocontrol of phytopathogenic fungi Alternaria spp." in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia), 42 (2018):169-169,
https://hdl.handle.net/21.15107/rcub_fiver_2533 .

TY  - JOUR
AU  - Spremo, Nemanja
AU  - Bešir, Nataša
AU  - Vraneš, Milan
AU  - Gadžurić, Slobodan
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Karaman, Maja
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2534
AB  - A modern sustainable approach in agricultural practice would
be a replacement of synthetic and toxic substances in the
treatment of crop with benign and environmentally friendly
compounds, by using principles of green chemistry. One of
the possible directions could be using of ionic liquids (ILs),
salts with melting points below 100 °C, which are well-known
for manifesting antimicrobial activity. ILs can be used in the
development of novel sources of antimicrobial agents such as
antiseptics, biocides and antifungal agents. Furthermore, ILs
have already been reported as alternative ‘green’ solvents for a
wide range of reactions and technological processes. Considering the importance of research in the area of bioactivity of
ILs for discovery of new green ILs for diff erent purposes, the
aim of this study was to examine antifungal activity of nine
newly synthesized ILs against phytopathogenic Alternaria
strains isolated from from rice (A. padwickii), carrot (A. dauci) and linseed (A. linicola). Antifungal eff ect was estimated
by micro-plate microdilution method for establishing minimum inhibitory (MIC) and minimum fungicidal concentration (MFC). All ionic liquids showed strain specifi c but good
antifungal activity on Alternaria strains with MIC and MFC
detected at the range from 9.23 mg/ml to 75.89 mg/ml. Only
in case of the 1-(4-hydroxy-2-oxy)butyl -3-methylimidazolium chloride [OHC2OC2mIm][Cl], no antifungal eff ect on
A. dauci were observed. Comparing to A. padwickii and A.
dauci., A. linicola showed higher sensitivity to all tested ILs.
Th e obtained results indicate the possibility of usage of ILs
in biocontrol of plant diseases, representing their application
in crop protection. However, further research is necessary in
order to examine their toxicity and biodegradability in the environment.
PB  - Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)
T1  - Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains
EP  - 169
IS  - 42
SP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2534
ER  - 

@article{
author = "Spremo, Nemanja and Bešir, Nataša and Vraneš, Milan and Gadžurić, Slobodan and Ignjatov, Maja and Bjelić, Dragana and Karaman, Maja",
year = "2018",
abstract = "A modern sustainable approach in agricultural practice would
be a replacement of synthetic and toxic substances in the
treatment of crop with benign and environmentally friendly
compounds, by using principles of green chemistry. One of
the possible directions could be using of ionic liquids (ILs),
salts with melting points below 100 °C, which are well-known
for manifesting antimicrobial activity. ILs can be used in the
development of novel sources of antimicrobial agents such as
antiseptics, biocides and antifungal agents. Furthermore, ILs
have already been reported as alternative ‘green’ solvents for a
wide range of reactions and technological processes. Considering the importance of research in the area of bioactivity of
ILs for discovery of new green ILs for diff erent purposes, the
aim of this study was to examine antifungal activity of nine
newly synthesized ILs against phytopathogenic Alternaria
strains isolated from from rice (A. padwickii), carrot (A. dauci) and linseed (A. linicola). Antifungal eff ect was estimated
by micro-plate microdilution method for establishing minimum inhibitory (MIC) and minimum fungicidal concentration (MFC). All ionic liquids showed strain specifi c but good
antifungal activity on Alternaria strains with MIC and MFC
detected at the range from 9.23 mg/ml to 75.89 mg/ml. Only
in case of the 1-(4-hydroxy-2-oxy)butyl -3-methylimidazolium chloride [OHC2OC2mIm][Cl], no antifungal eff ect on
A. dauci were observed. Comparing to A. padwickii and A.
dauci., A. linicola showed higher sensitivity to all tested ILs.
Th e obtained results indicate the possibility of usage of ILs
in biocontrol of plant diseases, representing their application
in crop protection. However, further research is necessary in
order to examine their toxicity and biodegradability in the environment.",
publisher = "Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)",
title = "Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains",
pages = "169-169",
number = "42",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2534"
}

Spremo, N., Bešir, N., Vraneš, M., Gadžurić, S., Ignjatov, M., Bjelić, D.,& Karaman, M.. (2018). Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains. in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia)
Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac.(42), 169-169.
https://hdl.handle.net/21.15107/rcub_fiver_2534

Spremo N, Bešir N, Vraneš M, Gadžurić S, Ignjatov M, Bjelić D, Karaman M. Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains. in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia). 2018;(42):169-169.
https://hdl.handle.net/21.15107/rcub_fiver_2534 .

Spremo, Nemanja, Bešir, Nataša, Vraneš, Milan, Gadžurić, Slobodan, Ignjatov, Maja, Bjelić, Dragana, Karaman, Maja, "Antifungal activities of ionic liquids against phytopathogenic Alternaria spp. strains" in Botanica Serbica, supplement 1 (Book of Abstracts, 7th Balkan Botanical Congress, 10-14 September 2018, Novi Sad, Serbia), no. 42 (2018):169-169,
https://hdl.handle.net/21.15107/rcub_fiver_2534 .

TY  - JOUR
AU  - Bjelić, Dragana
AU  - Ignjatov, Maja
AU  - Marinković, Jelena
AU  - Spremo, Nemanja R.
AU  - Karaman, Maja
AU  - Nikolić, Zorica
AU  - Ivanović, Žarko
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1650
AB  - Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.
AB  - Biokontrola fitopatogena predstavlja alternativu primeni pesticida, s obzirom da bakterije označene terminom PGPR (Plant Growth Promoting Rhizobacteria) stimulišu biljni rast i štite biljke od bolesti. Zahvaljujući sposobnosti da formiraju veoma rezistentne endospore i produkuju širok spektar antimikrobnih supstanci, vrste roda Bacillus su veoma zastupljene u zemljištu i pogodne za primenu u biotehnologiji. Cilj istraživanja bio je da se utvrdi antifungalna aktivnost jedanaest Bacillus spp. izolata iz zemljišta s različitih lokaliteta u Vojvodini. Sposobnost bakterijskih izolata da inhibiraju rast pet izolata gljiva ispitana je metodom dvojne kultivacije. Izolati Bacillus spp. ispoljili su najveću antifungalnu aktivnost prema Fusarium proliferatum, Fusarium oxysporum f. sp. cepae, i Alternaria padwickii, dok je najmanji antagonistički efekat utvrđen prema Fusarium verticillioides i Fusarium graminearum. Efektivni izolati identifikovani su kao Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) i Bacillus megaterium (B8, B9). Najveću antifungalnu aktivnost ispoljili su izolati B. subtilis B5 (39-62%) i B7 (40-71%). Ovi izolati mogu se koristiti kao potencijalni agensi za biološku kontrolu biljnih bolesti.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antifungal activity of indigenous Bacillus spp. isolated from soil
T1  - Antifungalna aktivnost prirodnih Bacillus spp. izolata iz zemljišta
EP  - 269
IS  - 133
SP  - 261
DO  - 10.2298/ZMSPN1733261B
ER  - 

@article{
author = "Bjelić, Dragana and Ignjatov, Maja and Marinković, Jelena and Spremo, Nemanja R. and Karaman, Maja and Nikolić, Zorica and Ivanović, Žarko",
year = "2017",
abstract = "Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases., Biokontrola fitopatogena predstavlja alternativu primeni pesticida, s obzirom da bakterije označene terminom PGPR (Plant Growth Promoting Rhizobacteria) stimulišu biljni rast i štite biljke od bolesti. Zahvaljujući sposobnosti da formiraju veoma rezistentne endospore i produkuju širok spektar antimikrobnih supstanci, vrste roda Bacillus su veoma zastupljene u zemljištu i pogodne za primenu u biotehnologiji. Cilj istraživanja bio je da se utvrdi antifungalna aktivnost jedanaest Bacillus spp. izolata iz zemljišta s različitih lokaliteta u Vojvodini. Sposobnost bakterijskih izolata da inhibiraju rast pet izolata gljiva ispitana je metodom dvojne kultivacije. Izolati Bacillus spp. ispoljili su najveću antifungalnu aktivnost prema Fusarium proliferatum, Fusarium oxysporum f. sp. cepae, i Alternaria padwickii, dok je najmanji antagonistički efekat utvrđen prema Fusarium verticillioides i Fusarium graminearum. Efektivni izolati identifikovani su kao Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) i Bacillus megaterium (B8, B9). Najveću antifungalnu aktivnost ispoljili su izolati B. subtilis B5 (39-62%) i B7 (40-71%). Ovi izolati mogu se koristiti kao potencijalni agensi za biološku kontrolu biljnih bolesti.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of indigenous Bacillus spp. isolated from soil, Antifungalna aktivnost prirodnih Bacillus spp. izolata iz zemljišta",
pages = "269-261",
number = "133",
doi = "10.2298/ZMSPN1733261B"
}

Bjelić, D., Ignjatov, M., Marinković, J., Spremo, N. R., Karaman, M., Nikolić, Z.,& Ivanović, Ž.. (2017). Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(133), 261-269.
https://doi.org/10.2298/ZMSPN1733261B

Bjelić D, Ignjatov M, Marinković J, Spremo NR, Karaman M, Nikolić Z, Ivanović Ž. Antifungal activity of indigenous Bacillus spp. isolated from soil. in Zbornik Matice srpske za prirodne nauke. 2017;(133):261-269.
doi:10.2298/ZMSPN1733261B .

Bjelić, Dragana, Ignjatov, Maja, Marinković, Jelena, Spremo, Nemanja R., Karaman, Maja, Nikolić, Zorica, Ivanović, Žarko, "Antifungal activity of indigenous Bacillus spp. isolated from soil" in Zbornik Matice srpske za prirodne nauke, no. 133 (2017):261-269,
https://doi.org/10.2298/ZMSPN1733261B . .

TY  - JOUR
AU  - Spremo, Nemanja R.
AU  - Tesanović, Kristina D.
AU  - Rakić, Milana S.
AU  - Janjušević, Ljiljana N.
AU  - Ignjatov, Maja
AU  - Bjelić, Dragana
AU  - Karaman, Maja
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1649
AB  - During the last decades, intensive application of synthetic fungicides in the agricultural crop protection practice caused growing concern for the existence of toxic chemical residues in food as well as in the whole environment. Instead of using synthetic fungicides, it is suggested that crop protection be carried out by using preparations based on compounds of natural origin (secondary metabolites of plants or microorganisms, including macrofungi from Basidiomycota) as biological control agents. The potential of macrofungal species as biocontrol agents was analyzed in this investigation of eight autochthonous species from different locations in Serbia. Both the terricolous species: Coprinus comatus, Coprinellus truncorum, Amanita strobiliformis, Hydnum repandum and the lignicolous species: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor and Bjerkandera adusta were examined, with an aim to detect some novel sources of antifungal agents. This study surveyed antifungal activity of selected macrofungal extracts (MeOH, EtOH and CHCl3) against phytopathogenic Fusarium and Alternaria strains isolated from garlic, soybean and rice: F. proliferatum, F. verticillioides, F. proliferatum, F. graminearum and A. padwickii. Microdilution method in 96 well micro-plates was applied for the estimation of antifungal effects of macrofungi extracts in the range from 24.75 to 198.00 mg/ml and determination of minimal inhibitory (MIC) and minimal fungicidal concentration (MFC). EtOH extract of mychorhizal species H. repandum showed antifungal activity against all analyzed phytopathogenic strains, with the strongest effect on Fusarium strains (MIC 24.75 mg/ml; MFC 24.75 mg/ml). Among others, MeOH extracts of S. subtomentosum and C. micaceus showed similar effects while only B. adusta showed slight effect on Fusarium strains (MIC 24.75-99.00 mg/ml; MFC 24.75-99.00 mg/ml) and none effect on A. padwickii. The obtained results indicate the possibility of using examined extracts as efficient antifungal agents and provide the basis for the new formulations of biocontrol agents against phytopathogenic fungi in the future.
AB  - Tokom poslednjih decenija intezivna primena sintetičkih fungicida u zaštiti poljoprivrednih useva izaziva sve veću zabrinutost ljudi zbog prisustva toksičnih hemijskih ostataka u prehrambenim proizvodima kao i celoj okolini. Umesto korišćenja sintetičkih fungicida, preporučuje se da se zaštita useva vrši korišćenjem preparata na bazi jedinjenja prirodnog porekla (sekundarni metaboliti biljaka ili mikroorganizama uključujući i makrogljive iz razdela Basidiomycota) kao agense biološke kontrole. U ovom radu istraživan je potencijal agenasa biološke kontrole za osam autohtonih vrsta makrogljiva s različitih lokaliteta u Srbiji. Obe, terikolne vrste: Coprinus comatus, Coprinellus truncorum, Amanita strobiliformis, Hydnum repandum i lignikolne vrste: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor i Bjerkandera adusta su istražene s ciljem da se otkriju neki novi izvori antifungalnih agenasa. U okviru ovog rada istražena je antifungalna aktivnost odabranih ekstrakata makrogljiva (metanolni, etanolni i hloroformski) protiv fitopatogenih sojeva Fusarium i Alternaria izolovanih s belog luka, soje i pirinča: F. proliferatum, F. verticillioides, F. proliferatum, F. graminearum i A. padwickii. Za procenu antifungalnog efekta ekstrakata makrogljiva upotrebljena je mikrodiluciona metoda mikrotitar pločama s 96 velova u opsegu koncentracija od 24,75 do 198,00 mg/ml i determinaciju minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije (MFC). Etanolni ekstrakt mikorizne vrste H. repandum pokazao je atifungalnu aktivnost prema svim analiziranim fitopatogenim sojevima, sa najjačim efektom prema sojevima Fusarium (MIC 24,75 mg/ml; MFC 24,75 mg/ml). Između ostalog, sličan efekat pokazali su i metanolni ekstrakti S. subtomentosum i C. micaceus, dok je samo B. adusta imala blagi efekat na sojeve Fusarium (MIC 24,75-99,00 mg/ml; MFC 24,75-99,00 mg/ml), ali ne i na A. padwickii. Dobijeni rezultati ukazuju na mogućnost korišćenja ispitanih ekstrakata kao veoma efikasnih antifungalnih agenasa i samim tim oni predstavljaju osnovu za nove formulacije biokontrolnih agenasa protiv fitopatogenih gljiva u budućnosti.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp.
T1  - Antifungalna aktivnost ekstrakata makrogljiva na fitopatogene sojeve gljiva rodova Fusarium sp. i Alternaria sp.
EP  - 240
IS  - 133
SP  - 231
DO  - 10.2298/ZMSPN1733231S
ER  - 

@article{
author = "Spremo, Nemanja R. and Tesanović, Kristina D. and Rakić, Milana S. and Janjušević, Ljiljana N. and Ignjatov, Maja and Bjelić, Dragana and Karaman, Maja",
year = "2017",
abstract = "During the last decades, intensive application of synthetic fungicides in the agricultural crop protection practice caused growing concern for the existence of toxic chemical residues in food as well as in the whole environment. Instead of using synthetic fungicides, it is suggested that crop protection be carried out by using preparations based on compounds of natural origin (secondary metabolites of plants or microorganisms, including macrofungi from Basidiomycota) as biological control agents. The potential of macrofungal species as biocontrol agents was analyzed in this investigation of eight autochthonous species from different locations in Serbia. Both the terricolous species: Coprinus comatus, Coprinellus truncorum, Amanita strobiliformis, Hydnum repandum and the lignicolous species: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor and Bjerkandera adusta were examined, with an aim to detect some novel sources of antifungal agents. This study surveyed antifungal activity of selected macrofungal extracts (MeOH, EtOH and CHCl3) against phytopathogenic Fusarium and Alternaria strains isolated from garlic, soybean and rice: F. proliferatum, F. verticillioides, F. proliferatum, F. graminearum and A. padwickii. Microdilution method in 96 well micro-plates was applied for the estimation of antifungal effects of macrofungi extracts in the range from 24.75 to 198.00 mg/ml and determination of minimal inhibitory (MIC) and minimal fungicidal concentration (MFC). EtOH extract of mychorhizal species H. repandum showed antifungal activity against all analyzed phytopathogenic strains, with the strongest effect on Fusarium strains (MIC 24.75 mg/ml; MFC 24.75 mg/ml). Among others, MeOH extracts of S. subtomentosum and C. micaceus showed similar effects while only B. adusta showed slight effect on Fusarium strains (MIC 24.75-99.00 mg/ml; MFC 24.75-99.00 mg/ml) and none effect on A. padwickii. The obtained results indicate the possibility of using examined extracts as efficient antifungal agents and provide the basis for the new formulations of biocontrol agents against phytopathogenic fungi in the future., Tokom poslednjih decenija intezivna primena sintetičkih fungicida u zaštiti poljoprivrednih useva izaziva sve veću zabrinutost ljudi zbog prisustva toksičnih hemijskih ostataka u prehrambenim proizvodima kao i celoj okolini. Umesto korišćenja sintetičkih fungicida, preporučuje se da se zaštita useva vrši korišćenjem preparata na bazi jedinjenja prirodnog porekla (sekundarni metaboliti biljaka ili mikroorganizama uključujući i makrogljive iz razdela Basidiomycota) kao agense biološke kontrole. U ovom radu istraživan je potencijal agenasa biološke kontrole za osam autohtonih vrsta makrogljiva s različitih lokaliteta u Srbiji. Obe, terikolne vrste: Coprinus comatus, Coprinellus truncorum, Amanita strobiliformis, Hydnum repandum i lignikolne vrste: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor i Bjerkandera adusta su istražene s ciljem da se otkriju neki novi izvori antifungalnih agenasa. U okviru ovog rada istražena je antifungalna aktivnost odabranih ekstrakata makrogljiva (metanolni, etanolni i hloroformski) protiv fitopatogenih sojeva Fusarium i Alternaria izolovanih s belog luka, soje i pirinča: F. proliferatum, F. verticillioides, F. proliferatum, F. graminearum i A. padwickii. Za procenu antifungalnog efekta ekstrakata makrogljiva upotrebljena je mikrodiluciona metoda mikrotitar pločama s 96 velova u opsegu koncentracija od 24,75 do 198,00 mg/ml i determinaciju minimalne inhibitorne (MIC) i minimalne fungicidne koncentracije (MFC). Etanolni ekstrakt mikorizne vrste H. repandum pokazao je atifungalnu aktivnost prema svim analiziranim fitopatogenim sojevima, sa najjačim efektom prema sojevima Fusarium (MIC 24,75 mg/ml; MFC 24,75 mg/ml). Između ostalog, sličan efekat pokazali su i metanolni ekstrakti S. subtomentosum i C. micaceus, dok je samo B. adusta imala blagi efekat na sojeve Fusarium (MIC 24,75-99,00 mg/ml; MFC 24,75-99,00 mg/ml), ali ne i na A. padwickii. Dobijeni rezultati ukazuju na mogućnost korišćenja ispitanih ekstrakata kao veoma efikasnih antifungalnih agenasa i samim tim oni predstavljaju osnovu za nove formulacije biokontrolnih agenasa protiv fitopatogenih gljiva u budućnosti.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp., Antifungalna aktivnost ekstrakata makrogljiva na fitopatogene sojeve gljiva rodova Fusarium sp. i Alternaria sp.",
pages = "240-231",
number = "133",
doi = "10.2298/ZMSPN1733231S"
}

Spremo, N. R., Tesanović, K. D., Rakić, M. S., Janjušević, L. N., Ignjatov, M., Bjelić, D.,& Karaman, M.. (2017). Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp.. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(133), 231-240.
https://doi.org/10.2298/ZMSPN1733231S

Spremo NR, Tesanović KD, Rakić MS, Janjušević LN, Ignjatov M, Bjelić D, Karaman M. Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp.. in Zbornik Matice srpske za prirodne nauke. 2017;(133):231-240.
doi:10.2298/ZMSPN1733231S .

Spremo, Nemanja R., Tesanović, Kristina D., Rakić, Milana S., Janjušević, Ljiljana N., Ignjatov, Maja, Bjelić, Dragana, Karaman, Maja, "Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp." in Zbornik Matice srpske za prirodne nauke, no. 133 (2017):231-240,
https://doi.org/10.2298/ZMSPN1733231S . .