TY  - JOUR
AU  - Tančić-Živanov, Sonja
AU  - Dedić, Boško
AU  - Dimitrijević, Aleksandra
AU  - Dušanić, Nenad
AU  - Jocić, Siniša
AU  - Miklič, Vladimir
AU  - Kovačević, Branislav
AU  - Miladinović, Dragana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1880
AB  - The study assessed the genetic variability among Macrophomina phaseolina (Tassi) Goid. isolates originating from six different countries (Turkey, Bulgaria, Romania, Spain, Ukraine and Serbia), collected mostly from sunflower, but also maize, soybean, flax, common bean and zebra plant. RAPD analysis was based on 154 polymorphic bands obtained with 14 OPA primers. RAPD markers OPA-02-OPA-05, OPA-07-OPA-13 and OPA-18-OPA-20 were found to be suitable for measuring the genetic relatedness and detecting variability in M. phaseolina populations. Cluster analysis revealed high genetic diversity of M. phaseolina isolates within the same country of origin, but clear differentiation according to the isolates' host plant and geographical origin was not observed. Contingency analysis and corrected contingency coefficients enabled determination of RAPD markers associated with geographical origin of isolates, as well as markers specific for non-sunflower isolates. Marker OPA 10_1750 was found to be highly specific for the isolates from Turkey, while two markers, OPA 3_1250 and OPA 9_800, could be used for differentiation of isolates originating from sunflower from isolates originating from other host plants.
PB  - Springer Heidelberg, Heidelberg
T2  - Journal of Plant Diseases & Protection
T1  - Analysis of genetic diversity among Macrophomina phaseolina (Tassi) Goid. isolates from Euro-Asian countries
EP  - 573
IS  - 6
SP  - 565
VL  - 126
DO  - 10.1007/s41348-019-00260-6
ER  - 

@article{
author = "Tančić-Živanov, Sonja and Dedić, Boško and Dimitrijević, Aleksandra and Dušanić, Nenad and Jocić, Siniša and Miklič, Vladimir and Kovačević, Branislav and Miladinović, Dragana",
year = "2019",
abstract = "The study assessed the genetic variability among Macrophomina phaseolina (Tassi) Goid. isolates originating from six different countries (Turkey, Bulgaria, Romania, Spain, Ukraine and Serbia), collected mostly from sunflower, but also maize, soybean, flax, common bean and zebra plant. RAPD analysis was based on 154 polymorphic bands obtained with 14 OPA primers. RAPD markers OPA-02-OPA-05, OPA-07-OPA-13 and OPA-18-OPA-20 were found to be suitable for measuring the genetic relatedness and detecting variability in M. phaseolina populations. Cluster analysis revealed high genetic diversity of M. phaseolina isolates within the same country of origin, but clear differentiation according to the isolates' host plant and geographical origin was not observed. Contingency analysis and corrected contingency coefficients enabled determination of RAPD markers associated with geographical origin of isolates, as well as markers specific for non-sunflower isolates. Marker OPA 10_1750 was found to be highly specific for the isolates from Turkey, while two markers, OPA 3_1250 and OPA 9_800, could be used for differentiation of isolates originating from sunflower from isolates originating from other host plants.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Journal of Plant Diseases & Protection",
title = "Analysis of genetic diversity among Macrophomina phaseolina (Tassi) Goid. isolates from Euro-Asian countries",
pages = "573-565",
number = "6",
volume = "126",
doi = "10.1007/s41348-019-00260-6"
}

Tančić-Živanov, S., Dedić, B., Dimitrijević, A., Dušanić, N., Jocić, S., Miklič, V., Kovačević, B.,& Miladinović, D.. (2019). Analysis of genetic diversity among Macrophomina phaseolina (Tassi) Goid. isolates from Euro-Asian countries. in Journal of Plant Diseases & Protection
Springer Heidelberg, Heidelberg., 126(6), 565-573.
https://doi.org/10.1007/s41348-019-00260-6

Tančić-Živanov S, Dedić B, Dimitrijević A, Dušanić N, Jocić S, Miklič V, Kovačević B, Miladinović D. Analysis of genetic diversity among Macrophomina phaseolina (Tassi) Goid. isolates from Euro-Asian countries. in Journal of Plant Diseases & Protection. 2019;126(6):565-573.
doi:10.1007/s41348-019-00260-6 .

Tančić-Živanov, Sonja, Dedić, Boško, Dimitrijević, Aleksandra, Dušanić, Nenad, Jocić, Siniša, Miklič, Vladimir, Kovačević, Branislav, Miladinović, Dragana, "Analysis of genetic diversity among Macrophomina phaseolina (Tassi) Goid. isolates from Euro-Asian countries" in Journal of Plant Diseases & Protection, 126, no. 6 (2019):565-573,
https://doi.org/10.1007/s41348-019-00260-6 . .

TY  - JOUR
AU  - Miladinović, Dragana
AU  - Miler, Marko
AU  - Marjanović-Jeromela, Ana
AU  - Imerovski, Ivana
AU  - Dimitrijević, Aleksandra
AU  - Kovačević, Branislav
AU  - Jocić, Siniša
AU  - Cvejić, Sandra
AU  - Hladni, Nada
AU  - Obreht-Vidaković, Dragana
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1786
AB  - Random amplification of polymorphic DNA (RAPD) analysis was performed on twelve rapeseed genotypes from Institute of Field and Vegetable Crops, Novi Sad, Serbia, genepool in order to identify markers that could be used in marker assisted selection (MAS) for different growing type and selection of the varieties with low or zero level of erucic acid. Out of fifteen RAPD markers, three were monomorphic, whereas twelve had polymorphic profiles. Three primers amplified specific fragments in spring varieties. UBC 25 and UBC 191 amplified the fragments of 450 and 750 bp, respectively, in all tested spring varieties, except in JR-NS-36. Primer UBC 72 generated a fragment of 700 bp that was present in all spring varieties. These fragments were not present in any of winter varieties. None of the tested RAPD primers amplified fragment(s) uniquely present either in varieties with or without (0%) erucic acid or with different erucic acid content. Cluster analysis showed a concordance between the position of varieties in the cluster and their pedigree information, but also enabled separation of spring and winter varieties. Contingency analysis confirmed that fragment UBC 72_700 is specific for spring varieties, while for erucic acid content, only moderate association was found with UBC 137_750.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed
EP  - 430
IS  - 2
SP  - 421
VL  - 50
DO  - 10.2298/GENSR1802421M
ER  - 

@article{
author = "Miladinović, Dragana and Miler, Marko and Marjanović-Jeromela, Ana and Imerovski, Ivana and Dimitrijević, Aleksandra and Kovačević, Branislav and Jocić, Siniša and Cvejić, Sandra and Hladni, Nada and Obreht-Vidaković, Dragana",
year = "2018",
abstract = "Random amplification of polymorphic DNA (RAPD) analysis was performed on twelve rapeseed genotypes from Institute of Field and Vegetable Crops, Novi Sad, Serbia, genepool in order to identify markers that could be used in marker assisted selection (MAS) for different growing type and selection of the varieties with low or zero level of erucic acid. Out of fifteen RAPD markers, three were monomorphic, whereas twelve had polymorphic profiles. Three primers amplified specific fragments in spring varieties. UBC 25 and UBC 191 amplified the fragments of 450 and 750 bp, respectively, in all tested spring varieties, except in JR-NS-36. Primer UBC 72 generated a fragment of 700 bp that was present in all spring varieties. These fragments were not present in any of winter varieties. None of the tested RAPD primers amplified fragment(s) uniquely present either in varieties with or without (0%) erucic acid or with different erucic acid content. Cluster analysis showed a concordance between the position of varieties in the cluster and their pedigree information, but also enabled separation of spring and winter varieties. Contingency analysis confirmed that fragment UBC 72_700 is specific for spring varieties, while for erucic acid content, only moderate association was found with UBC 137_750.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed",
pages = "430-421",
number = "2",
volume = "50",
doi = "10.2298/GENSR1802421M"
}

Miladinović, D., Miler, M., Marjanović-Jeromela, A., Imerovski, I., Dimitrijević, A., Kovačević, B., Jocić, S., Cvejić, S., Hladni, N.,& Obreht-Vidaković, D.. (2018). Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 50(2), 421-430.
https://doi.org/10.2298/GENSR1802421M

Miladinović D, Miler M, Marjanović-Jeromela A, Imerovski I, Dimitrijević A, Kovačević B, Jocić S, Cvejić S, Hladni N, Obreht-Vidaković D. Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed. in Genetika-Belgrade. 2018;50(2):421-430.
doi:10.2298/GENSR1802421M .

Miladinović, Dragana, Miler, Marko, Marjanović-Jeromela, Ana, Imerovski, Ivana, Dimitrijević, Aleksandra, Kovačević, Branislav, Jocić, Siniša, Cvejić, Sandra, Hladni, Nada, Obreht-Vidaković, Dragana, "Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed" in Genetika-Belgrade, 50, no. 2 (2018):421-430,
https://doi.org/10.2298/GENSR1802421M . .

TY  - JOUR
AU  - Vuksanović, Vanja
AU  - Kovačević, Branislav
AU  - Orlović, Saša
AU  - Miladinović, Dragana
AU  - Kebert, Marko
AU  - Katanić, Marina
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1647
AB  - In this study, changes in the pH of the nutrient medium were investigated after 35 days of cultivation of four white poplar genotypes (Populus alba L.). Four initial pH values of the substrate were tested: 3.0, 4.0, 5.5 and 7.0 buffered sodium citrate buffer, as well as a standard medium for micropropagation with pH 5.5 without the addition of citric acid (control) The medium was sterilized in a microwave oven. The results of the variance analysis indicate that the final pH of the medium after cultivation, as well as the difference between the final and the initial pH of the medium, was significantly influence of initial pH, as well as the interaction of the genotype × medium, differences between genotypes in their reaction to the washed pH medium. On media with low initial pH was recorded positive, while the medium pH 7.0 was recorded negative change pH after cultivation. The results of the study indicate that changes in the pH of the medium during cultivation lead to a pH that is assumed to be optimal for their cultivation under in vitro conditions (cca. pH 5.5). The possibility of applying the obtained results in the improvement of the white poplar cultivation in vitro and the assessment of genotypes in melioration and phytooremediation projects is discussed.
AB  - U ovom istraživanju je ispitivana promena pH vrednosti hranljive podloge nakon 35 dana kultivacije četiri genotipa bele topole (Populus alba L.). Ispitane su četiri početne pH vrednosti podloga: 3,0, 4,0, 5,5 i 7,0 puferisane natrijum citratnim puferom, kao i standardna podloga za umnožavanje sa pH 5,5 bez dodavanja limunske kiseline (kontrola). Sterilizacija podloge je izvršena u mikrotalasnoj pećnici. Rezultati analize varijanse ukazuju da su konačni pH podloge nakon kultivacije, kao i razlika između krajnje i početne pH podloge veoma značajno zavisili od početne pH. Takođe je i interakcija genotip × podloga pokazala statistički znacajan uticaj na pomenuta svojstva. Na podlogama sa niskom početnom pH je zabeležena pozitivna, dok je na podlozi pH 7,0 zabeležena negativna promena pH tokom kultivacije. Rezultati istraživanja ukazuju da promene pH podloge tokom kultivacije vode ka vrednosti pH 5,5 za koju pretpostavljamo da bi bila optimalna za njihov uzgoj u uslovima in vitro (oko pH 5,5). Diskutovana je mogućnost primene dobijenih rezulata u unapređenju uzgoja bele topole in vitro i ocene genotipova u projektima melioracije i fitoremedijacije.
PB  - Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad
T2  - Topola
T1  - Changes in medium pH during white poplar micropropagation
T1  - Promene pH vrednosti podloge prilikom mikropropagacije bele topole
EP  - 165
IS  - 199-200
SP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1647
ER  - 

@article{
author = "Vuksanović, Vanja and Kovačević, Branislav and Orlović, Saša and Miladinović, Dragana and Kebert, Marko and Katanić, Marina",
year = "2017",
abstract = "In this study, changes in the pH of the nutrient medium were investigated after 35 days of cultivation of four white poplar genotypes (Populus alba L.). Four initial pH values of the substrate were tested: 3.0, 4.0, 5.5 and 7.0 buffered sodium citrate buffer, as well as a standard medium for micropropagation with pH 5.5 without the addition of citric acid (control) The medium was sterilized in a microwave oven. The results of the variance analysis indicate that the final pH of the medium after cultivation, as well as the difference between the final and the initial pH of the medium, was significantly influence of initial pH, as well as the interaction of the genotype × medium, differences between genotypes in their reaction to the washed pH medium. On media with low initial pH was recorded positive, while the medium pH 7.0 was recorded negative change pH after cultivation. The results of the study indicate that changes in the pH of the medium during cultivation lead to a pH that is assumed to be optimal for their cultivation under in vitro conditions (cca. pH 5.5). The possibility of applying the obtained results in the improvement of the white poplar cultivation in vitro and the assessment of genotypes in melioration and phytooremediation projects is discussed., U ovom istraživanju je ispitivana promena pH vrednosti hranljive podloge nakon 35 dana kultivacije četiri genotipa bele topole (Populus alba L.). Ispitane su četiri početne pH vrednosti podloga: 3,0, 4,0, 5,5 i 7,0 puferisane natrijum citratnim puferom, kao i standardna podloga za umnožavanje sa pH 5,5 bez dodavanja limunske kiseline (kontrola). Sterilizacija podloge je izvršena u mikrotalasnoj pećnici. Rezultati analize varijanse ukazuju da su konačni pH podloge nakon kultivacije, kao i razlika između krajnje i početne pH podloge veoma značajno zavisili od početne pH. Takođe je i interakcija genotip × podloga pokazala statistički znacajan uticaj na pomenuta svojstva. Na podlogama sa niskom početnom pH je zabeležena pozitivna, dok je na podlozi pH 7,0 zabeležena negativna promena pH tokom kultivacije. Rezultati istraživanja ukazuju da promene pH podloge tokom kultivacije vode ka vrednosti pH 5,5 za koju pretpostavljamo da bi bila optimalna za njihov uzgoj u uslovima in vitro (oko pH 5,5). Diskutovana je mogućnost primene dobijenih rezulata u unapređenju uzgoja bele topole in vitro i ocene genotipova u projektima melioracije i fitoremedijacije.",
publisher = "Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad",
journal = "Topola",
title = "Changes in medium pH during white poplar micropropagation, Promene pH vrednosti podloge prilikom mikropropagacije bele topole",
pages = "165-153",
number = "199-200",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1647"
}

Vuksanović, V., Kovačević, B., Orlović, S., Miladinović, D., Kebert, M.,& Katanić, M.. (2017). Changes in medium pH during white poplar micropropagation. in Topola
Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad.(199-200), 153-165.
https://hdl.handle.net/21.15107/rcub_fiver_1647

Vuksanović V, Kovačević B, Orlović S, Miladinović D, Kebert M, Katanić M. Changes in medium pH during white poplar micropropagation. in Topola. 2017;(199-200):153-165.
https://hdl.handle.net/21.15107/rcub_fiver_1647 .

Vuksanović, Vanja, Kovačević, Branislav, Orlović, Saša, Miladinović, Dragana, Kebert, Marko, Katanić, Marina, "Changes in medium pH during white poplar micropropagation" in Topola, no. 199-200 (2017):153-165,
https://hdl.handle.net/21.15107/rcub_fiver_1647 .

TY  - JOUR
AU  - Vuksanović, Vanja
AU  - Kovačević, Branislav
AU  - Katanić, Marina
AU  - Orlović, Saša
AU  - Miladinović, Dragana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1694
AB  - Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10(-3), 10(-4) and 10(-7) M ( buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10(-7) M Cu2+. As the medium with 10(-3) M Cu2+ exhibited a toxic effect, the effect of 10(-4) M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra
EP  - 687
IS  - 4
SP  - 679
VL  - 69
DO  - 10.2298/ABS170210014V
ER  - 

@article{
author = "Vuksanović, Vanja and Kovačević, Branislav and Katanić, Marina and Orlović, Saša and Miladinović, Dragana",
year = "2017",
abstract = "Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10(-3), 10(-4) and 10(-7) M ( buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10(-7) M Cu2+. As the medium with 10(-3) M Cu2+ exhibited a toxic effect, the effect of 10(-4) M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra",
pages = "687-679",
number = "4",
volume = "69",
doi = "10.2298/ABS170210014V"
}

Vuksanović, V., Kovačević, B., Katanić, M., Orlović, S.,& Miladinović, D.. (2017). In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 69(4), 679-687.
https://doi.org/10.2298/ABS170210014V

Vuksanović V, Kovačević B, Katanić M, Orlović S, Miladinović D. In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. in Archives of Biological Sciences. 2017;69(4):679-687.
doi:10.2298/ABS170210014V .

Vuksanović, Vanja, Kovačević, Branislav, Katanić, Marina, Orlović, Saša, Miladinović, Dragana, "In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra" in Archives of Biological Sciences, 69, no. 4 (2017):679-687,
https://doi.org/10.2298/ABS170210014V . .

TY  - CONF
AU  - Miladinović, Dragana
AU  - Kovačević, Branislav
AU  - Jocić, Siniša
AU  - Imerovski, Ivana
AU  - Dimitrijević, Aleksandra
AU  - Cvejić, Sandra
AU  - Balalić, Igor
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/2790
AB  - The success of anther culture depends on numerous factors such as genotype, donor plant growing conditions, anther pre-treatment and development stage, as well as incubation conditions. We have investigated the effect of sowing date and sowing density of donor plants on callus induction and shoot regeneration from cultivated sunflower anthers. Anthers were collected from of three commercial sunflower hybrids that were sown in four different sowing dates, and at three different sowing densities. Anthers were surface sterilized and placed on MS-medium based solid regeneration media. The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analysed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA. Statistical analysis enabled us to determine effect of sowing date and density on anther culture and shoot regeneration induction. Sowing date had a significant effect on all observed parameters, with earlier sowing dates having significant positive effect on shoot regeneration. Sowing density had no effect on either of observed parameters in all tested genotypes. The obtained results will contribute to the better understanding of the conditions needed for haploid production in sunflower and its introduction in sunflower breeding programs.
PB  - Paris : International Sunflower Association
C3  - Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey
T1  - The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers
EP  - 514
SP  - 509
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2790
ER  - 

@conference{
author = "Miladinović, Dragana and Kovačević, Branislav and Jocić, Siniša and Imerovski, Ivana and Dimitrijević, Aleksandra and Cvejić, Sandra and Balalić, Igor",
year = "2016",
abstract = "The success of anther culture depends on numerous factors such as genotype, donor plant growing conditions, anther pre-treatment and development stage, as well as incubation conditions. We have investigated the effect of sowing date and sowing density of donor plants on callus induction and shoot regeneration from cultivated sunflower anthers. Anthers were collected from of three commercial sunflower hybrids that were sown in four different sowing dates, and at three different sowing densities. Anthers were surface sterilized and placed on MS-medium based solid regeneration media. The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analysed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA. Statistical analysis enabled us to determine effect of sowing date and density on anther culture and shoot regeneration induction. Sowing date had a significant effect on all observed parameters, with earlier sowing dates having significant positive effect on shoot regeneration. Sowing density had no effect on either of observed parameters in all tested genotypes. The obtained results will contribute to the better understanding of the conditions needed for haploid production in sunflower and its introduction in sunflower breeding programs.",
publisher = "Paris : International Sunflower Association",
journal = "Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey",
title = "The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers",
pages = "514-509",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2790"
}

Miladinović, D., Kovačević, B., Jocić, S., Imerovski, I., Dimitrijević, A., Cvejić, S.,& Balalić, I.. (2016). The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers. in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey
Paris : International Sunflower Association., 509-514.
https://hdl.handle.net/21.15107/rcub_fiver_2790

Miladinović D, Kovačević B, Jocić S, Imerovski I, Dimitrijević A, Cvejić S, Balalić I. The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers. in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey. 2016;:509-514.
https://hdl.handle.net/21.15107/rcub_fiver_2790 .

Miladinović, Dragana, Kovačević, Branislav, Jocić, Siniša, Imerovski, Ivana, Dimitrijević, Aleksandra, Cvejić, Sandra, Balalić, Igor, "The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers" in Proceedings, 19th International Sunflower Conference, 29 May - 3 June 2016, Edirne, Turkey (2016):509-514,
https://hdl.handle.net/21.15107/rcub_fiver_2790 .

TY  - JOUR
AU  - Dimitrijević, Aleksandra
AU  - Imerovski, Ivana
AU  - Miladinović, Dragana
AU  - Jocić, Siniša
AU  - Cvejić, Sandra
AU  - Malidža, Goran
AU  - Kovačević, Branislav
AU  - Šurlan-Momirović, Gordana
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1526
AB  - A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively.
AB  - Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - A rapid test for detection of tribenuron-methyl resistance in sunflower
T1  - Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil
EP  - 8
IS  - 1
SP  - 1
VL  - 53
DO  - 10.5937/ratpov53-8412
ER  - 

@article{
author = "Dimitrijević, Aleksandra and Imerovski, Ivana and Miladinović, Dragana and Jocić, Siniša and Cvejić, Sandra and Malidža, Goran and Kovačević, Branislav and Šurlan-Momirović, Gordana",
year = "2016",
abstract = "A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively., Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "A rapid test for detection of tribenuron-methyl resistance in sunflower, Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil",
pages = "8-1",
number = "1",
volume = "53",
doi = "10.5937/ratpov53-8412"
}

Dimitrijević, A., Imerovski, I., Miladinović, D., Jocić, S., Cvejić, S., Malidža, G., Kovačević, B.,& Šurlan-Momirović, G.. (2016). A rapid test for detection of tribenuron-methyl resistance in sunflower. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 53(1), 1-8.
https://doi.org/10.5937/ratpov53-8412

Dimitrijević A, Imerovski I, Miladinović D, Jocić S, Cvejić S, Malidža G, Kovačević B, Šurlan-Momirović G. A rapid test for detection of tribenuron-methyl resistance in sunflower. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2016;53(1):1-8.
doi:10.5937/ratpov53-8412 .

Dimitrijević, Aleksandra, Imerovski, Ivana, Miladinović, Dragana, Jocić, Siniša, Cvejić, Sandra, Malidža, Goran, Kovačević, Branislav, Šurlan-Momirović, Gordana, "A rapid test for detection of tribenuron-methyl resistance in sunflower" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 53, no. 1 (2016):1-8,
https://doi.org/10.5937/ratpov53-8412 . .

TY  - JOUR
AU  - Miladinović, Dragana
AU  - Taški-Ajduković, Ksenija
AU  - Nagl, Nevena
AU  - Kovačević, Branislav
AU  - Dimitrijević, Aleksandra
AU  - Imerovski, Ivana
AU  - Miladinović, Jegor
AU  - Dušanić, Nenad
AU  - Balalić, Igor
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1323
AB  - Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism among accessions of wild sunflower species Helianthus maximiliani, Helianthus tuberosus, Helianthus mollis and Helianthus rigidus with different tolerance to mid-stalk white rot and selection of potential markers for different levels of tolerance to this disease. Estimates of genetic variation showed that genetic diversity was equally distributed between Helianthus species and within them. Cluster analysis corresponded to the phylogenetic relations within the genus Helianthus. The results obtained by principal coordinates analysis (PCoA), where the first two principal coordinates accounted for 83.7% of total variation, perfectly coincided with the results of cluster analysis. Contingency coefficient significance test showed that most of the used primers generated bands associated with some level of tolerance or susceptibility to mid-stalk white rot. Furthermore, contingency analysis showed that primer C12 generated bands associated with resistance (100%) to mid-stalk white rot both in H. mollis and in all accessions, while primer X18 generated bands significantly associated with high tolerance (75%) in H. rigidus, H. mollis as well as in all tested accessions. The C15-600 bp locus was found to be significantly associated with high tolerance (75%) in all accessions, and medium tolerance (50%) in H. mollis.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot
EP  - 342
IS  - 2
SP  - 331
VL  - 46
DO  - 10.2298/GENSR1402331M
ER  - 

@article{
author = "Miladinović, Dragana and Taški-Ajduković, Ksenija and Nagl, Nevena and Kovačević, Branislav and Dimitrijević, Aleksandra and Imerovski, Ivana and Miladinović, Jegor and Dušanić, Nenad and Balalić, Igor",
year = "2014",
abstract = "Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism among accessions of wild sunflower species Helianthus maximiliani, Helianthus tuberosus, Helianthus mollis and Helianthus rigidus with different tolerance to mid-stalk white rot and selection of potential markers for different levels of tolerance to this disease. Estimates of genetic variation showed that genetic diversity was equally distributed between Helianthus species and within them. Cluster analysis corresponded to the phylogenetic relations within the genus Helianthus. The results obtained by principal coordinates analysis (PCoA), where the first two principal coordinates accounted for 83.7% of total variation, perfectly coincided with the results of cluster analysis. Contingency coefficient significance test showed that most of the used primers generated bands associated with some level of tolerance or susceptibility to mid-stalk white rot. Furthermore, contingency analysis showed that primer C12 generated bands associated with resistance (100%) to mid-stalk white rot both in H. mollis and in all accessions, while primer X18 generated bands significantly associated with high tolerance (75%) in H. rigidus, H. mollis as well as in all tested accessions. The C15-600 bp locus was found to be significantly associated with high tolerance (75%) in all accessions, and medium tolerance (50%) in H. mollis.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot",
pages = "342-331",
number = "2",
volume = "46",
doi = "10.2298/GENSR1402331M"
}

Miladinović, D., Taški-Ajduković, K., Nagl, N., Kovačević, B., Dimitrijević, A., Imerovski, I., Miladinović, J., Dušanić, N.,& Balalić, I.. (2014). Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 46(2), 331-342.
https://doi.org/10.2298/GENSR1402331M

Miladinović D, Taški-Ajduković K, Nagl N, Kovačević B, Dimitrijević A, Imerovski I, Miladinović J, Dušanić N, Balalić I. Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot. in Genetika-Belgrade. 2014;46(2):331-342.
doi:10.2298/GENSR1402331M .

Miladinović, Dragana, Taški-Ajduković, Ksenija, Nagl, Nevena, Kovačević, Branislav, Dimitrijević, Aleksandra, Imerovski, Ivana, Miladinović, Jegor, Dušanić, Nenad, Balalić, Igor, "Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot" in Genetika-Belgrade, 46, no. 2 (2014):331-342,
https://doi.org/10.2298/GENSR1402331M . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Kevrešan, Slavko
AU  - Orlović, Saša
AU  - Miladinović, Dragana
AU  - Novta-Ćitin Vera S.
AU  - Kuhajda, Ksenija
AU  - Katanić, Marina
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1338
AB  - The study describes the rooting effect of naphthenates and their fractions on in vitro grown Robinia pseudoacacia L. shoots. Natural naphthenic acids have been isolated by alkaline extraction from middle fraction of crude oil type “Velebit” from Vojvodina, characterized and fractionated. Black locust shoot bases were immersed in ACM medium [Ahuja, 1984] without agar supplemented with either 10, 50 or 100 µM of basic naphthenate preparation, naphthenate fractions obtained by extraction at different pHs (pH 2, pH 4, pH 7 and pH 9), or indole3-butyric acid (IBA). Treated shoots have been then grown on hormone-free medium for four weeks. Significant differences among test treatments were recorded during the third and the fourth week of in vitro cultivation. Final evaluation was performed on the basis of rooting percentage after four weeks of cultivation. The highest rooting percentage (>70%) was achieved after the treatment with solution containing 50 µM of IBA. However, treatment with 10 µM of naphthenate preparation achieved also positive effect on rooting (>60%). Average rooting percentage in the control treatment was just 45%. Our results with black locust confirm previous results gained with some other agricultural and forest tree species that naphthenates have the potential to stimulate rooting in shoots and cuttings.
AB  - Rad opisuje efekat naftenata na ožiljavanje mikroizbojaka bagrema in vitro. Naftenske kiseline su izolovane baznom ekstrakcijom iz srednje frakcije sirove nafte tipa “Velebit”, koja je opisana u ranijim radovima. Donji deo mikroizbojka je uronjen jedan minut u tečni ACM medijum [Ahuja, 1984] u koji je dodato 10, 50 ili 100 µM osnovne mešavine natrijum-naftenata ili njenih pojedinih frakcija dobijenih ekstrakcijom na različitim pH (pH 2, pH 4, pH 7 ili pH 9), odnosno 10, 50, 100 µM ili 1g/l indol-3-buterne kiseline (IBA). Kontrolni tretman je činio ACM medijum bez ispitivanih aktivnih materija. Tretirani mikroizbojci su zatim gajeni na čvrstoj ACM podlozi bez hormona. Značajne razlike među ispitivanim tretmanima su zabeležene tokom treće i četvrte nedelje uzgoja u in vitro uslovima. Konačna ocena je izvedena na osnovu procenta ožiljavanja nakon četiri nedelje uzgoja. Najviši procenat ožiljavanja je postignut rastvorom sa 10 µM natrijum naftenata, nakon čega je ostvaren značajan pozitivan efekat na procenat ožiljavanja (>60%) u odnosu na kontrolni tretman (oko 45%). Rezultati do kojih smo došli kod bagrema potvrđuju ranije rezultate koji su dobijeni kod poljoprivrednih i šumskih drvenastih vrsta o mogućnosti stimulacije ožiljavanja mikroizbojaka i reznica solima naftenskih kiselina.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Naphthenic acids: Alternative rooting stimulators in black locust microshoots
T1  - Naftenske kiseline - alternativni stimulatori ožiljavanja kod mikroizbojaka bagrema
EP  - 13
IS  - 126
SP  - 7
DO  - 10.2298/zmspn1426007K
ER  - 

@article{
author = "Kovačević, Branislav and Kevrešan, Slavko and Orlović, Saša and Miladinović, Dragana and Novta-Ćitin Vera S. and Kuhajda, Ksenija and Katanić, Marina",
year = "2014",
abstract = "The study describes the rooting effect of naphthenates and their fractions on in vitro grown Robinia pseudoacacia L. shoots. Natural naphthenic acids have been isolated by alkaline extraction from middle fraction of crude oil type “Velebit” from Vojvodina, characterized and fractionated. Black locust shoot bases were immersed in ACM medium [Ahuja, 1984] without agar supplemented with either 10, 50 or 100 µM of basic naphthenate preparation, naphthenate fractions obtained by extraction at different pHs (pH 2, pH 4, pH 7 and pH 9), or indole3-butyric acid (IBA). Treated shoots have been then grown on hormone-free medium for four weeks. Significant differences among test treatments were recorded during the third and the fourth week of in vitro cultivation. Final evaluation was performed on the basis of rooting percentage after four weeks of cultivation. The highest rooting percentage (>70%) was achieved after the treatment with solution containing 50 µM of IBA. However, treatment with 10 µM of naphthenate preparation achieved also positive effect on rooting (>60%). Average rooting percentage in the control treatment was just 45%. Our results with black locust confirm previous results gained with some other agricultural and forest tree species that naphthenates have the potential to stimulate rooting in shoots and cuttings., Rad opisuje efekat naftenata na ožiljavanje mikroizbojaka bagrema in vitro. Naftenske kiseline su izolovane baznom ekstrakcijom iz srednje frakcije sirove nafte tipa “Velebit”, koja je opisana u ranijim radovima. Donji deo mikroizbojka je uronjen jedan minut u tečni ACM medijum [Ahuja, 1984] u koji je dodato 10, 50 ili 100 µM osnovne mešavine natrijum-naftenata ili njenih pojedinih frakcija dobijenih ekstrakcijom na različitim pH (pH 2, pH 4, pH 7 ili pH 9), odnosno 10, 50, 100 µM ili 1g/l indol-3-buterne kiseline (IBA). Kontrolni tretman je činio ACM medijum bez ispitivanih aktivnih materija. Tretirani mikroizbojci su zatim gajeni na čvrstoj ACM podlozi bez hormona. Značajne razlike među ispitivanim tretmanima su zabeležene tokom treće i četvrte nedelje uzgoja u in vitro uslovima. Konačna ocena je izvedena na osnovu procenta ožiljavanja nakon četiri nedelje uzgoja. Najviši procenat ožiljavanja je postignut rastvorom sa 10 µM natrijum naftenata, nakon čega je ostvaren značajan pozitivan efekat na procenat ožiljavanja (>60%) u odnosu na kontrolni tretman (oko 45%). Rezultati do kojih smo došli kod bagrema potvrđuju ranije rezultate koji su dobijeni kod poljoprivrednih i šumskih drvenastih vrsta o mogućnosti stimulacije ožiljavanja mikroizbojaka i reznica solima naftenskih kiselina.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Naphthenic acids: Alternative rooting stimulators in black locust microshoots, Naftenske kiseline - alternativni stimulatori ožiljavanja kod mikroizbojaka bagrema",
pages = "13-7",
number = "126",
doi = "10.2298/zmspn1426007K"
}

Kovačević, B., Kevrešan, S., Orlović, S., Miladinović, D., Novta-Ćitin Vera S., Kuhajda, K.,& Katanić, M.. (2014). Naphthenic acids: Alternative rooting stimulators in black locust microshoots. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(126), 7-13.
https://doi.org/10.2298/zmspn1426007K

Kovačević B, Kevrešan S, Orlović S, Miladinović D, Novta-Ćitin Vera S., Kuhajda K, Katanić M. Naphthenic acids: Alternative rooting stimulators in black locust microshoots. in Zbornik Matice srpske za prirodne nauke. 2014;(126):7-13.
doi:10.2298/zmspn1426007K .

Kovačević, Branislav, Kevrešan, Slavko, Orlović, Saša, Miladinović, Dragana, Novta-Ćitin Vera S., Kuhajda, Ksenija, Katanić, Marina, "Naphthenic acids: Alternative rooting stimulators in black locust microshoots" in Zbornik Matice srpske za prirodne nauke, no. 126 (2014):7-13,
https://doi.org/10.2298/zmspn1426007K . .

TY  - JOUR
AU  - Imerovski, Ivana
AU  - Dimitrijević, Aleksandra
AU  - Miladinović, Dragana
AU  - Dedić, Boško
AU  - Jocić, Siniša
AU  - Kovačević, Branislav
AU  - Obreht, Dragana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1244
AB  - Broomrape is a parasitic plant that significantly decreases yield of sunflower. Breeding for resistance has proved to be the most efficient method for suppressing broomrape infestation in the field; however, new races of parasite constantly emerge, and new resistance genes need to be discovered and introduced into cultivated sunflower lines. The aim of this work was to test SSR markers from linkage group 3 (LG3) to investigate whether they could be used for identification of a particular Or gene. Twenty sunflower inbred lines were used, and polymorphism between the lines with various resistance genes and genetic background was investigated. The used markers revealed DNA polymorphism between the investigated lines. Strong association of markers from LG3 with Or6, as well as Or4 and Or2 genes, was found. Identified markers could be used for introduction of these resistance genes into commercial sunflower lines and for establishment and identification of differential lines.
PB  - Wiley, Hoboken
T2  - Plant Breeding
T1  - Identification of PCR markers linked to different Or genes in sunflower
EP  - 120
IS  - 1
SP  - 115
VL  - 132
DO  - 10.1111/pbr.12022
ER  - 

@article{
author = "Imerovski, Ivana and Dimitrijević, Aleksandra and Miladinović, Dragana and Dedić, Boško and Jocić, Siniša and Kovačević, Branislav and Obreht, Dragana",
year = "2013",
abstract = "Broomrape is a parasitic plant that significantly decreases yield of sunflower. Breeding for resistance has proved to be the most efficient method for suppressing broomrape infestation in the field; however, new races of parasite constantly emerge, and new resistance genes need to be discovered and introduced into cultivated sunflower lines. The aim of this work was to test SSR markers from linkage group 3 (LG3) to investigate whether they could be used for identification of a particular Or gene. Twenty sunflower inbred lines were used, and polymorphism between the lines with various resistance genes and genetic background was investigated. The used markers revealed DNA polymorphism between the investigated lines. Strong association of markers from LG3 with Or6, as well as Or4 and Or2 genes, was found. Identified markers could be used for introduction of these resistance genes into commercial sunflower lines and for establishment and identification of differential lines.",
publisher = "Wiley, Hoboken",
journal = "Plant Breeding",
title = "Identification of PCR markers linked to different Or genes in sunflower",
pages = "120-115",
number = "1",
volume = "132",
doi = "10.1111/pbr.12022"
}

Imerovski, I., Dimitrijević, A., Miladinović, D., Dedić, B., Jocić, S., Kovačević, B.,& Obreht, D.. (2013). Identification of PCR markers linked to different Or genes in sunflower. in Plant Breeding
Wiley, Hoboken., 132(1), 115-120.
https://doi.org/10.1111/pbr.12022

Imerovski I, Dimitrijević A, Miladinović D, Dedić B, Jocić S, Kovačević B, Obreht D. Identification of PCR markers linked to different Or genes in sunflower. in Plant Breeding. 2013;132(1):115-120.
doi:10.1111/pbr.12022 .

Imerovski, Ivana, Dimitrijević, Aleksandra, Miladinović, Dragana, Dedić, Boško, Jocić, Siniša, Kovačević, Branislav, Obreht, Dragana, "Identification of PCR markers linked to different Or genes in sunflower" in Plant Breeding, 132, no. 1 (2013):115-120,
https://doi.org/10.1111/pbr.12022 . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Miladinović, Dragana
AU  - Orlović, Saša
AU  - Katanić, Marina
AU  - Kebert, Marko
AU  - Kovinčić, Jovana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1294
AB  - Background and Purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation.
Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO3)2 concentrations were used: 0, 10-6, 10-5, 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content.
Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO3)2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO3)2 media. Thus, the concentration of 10-4 M Pb(NO3)2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype “Villafranca” (almost 200% and 125% higher, respectively).
PB  - Croatian Forest Research Institute
T2  - South-East European Forestry
T1  - Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro
EP  - 12
IS  - 1
SP  - 3
VL  - 4
DO  - 10.15177/seefor.13-01
ER  - 

@article{
author = "Kovačević, Branislav and Miladinović, Dragana and Orlović, Saša and Katanić, Marina and Kebert, Marko and Kovinčić, Jovana",
year = "2013",
abstract = "Background and Purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation.
Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO3)2 concentrations were used: 0, 10-6, 10-5, 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content.
Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO3)2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO3)2 media. Thus, the concentration of 10-4 M Pb(NO3)2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype “Villafranca” (almost 200% and 125% higher, respectively).",
publisher = "Croatian Forest Research Institute",
journal = "South-East European Forestry",
title = "Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro",
pages = "12-3",
number = "1",
volume = "4",
doi = "10.15177/seefor.13-01"
}

Kovačević, B., Miladinović, D., Orlović, S., Katanić, M., Kebert, M.,& Kovinčić, J.. (2013). Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro. in South-East European Forestry
Croatian Forest Research Institute., 4(1), 3-12.
https://doi.org/10.15177/seefor.13-01

Kovačević B, Miladinović D, Orlović S, Katanić M, Kebert M, Kovinčić J. Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro. in South-East European Forestry. 2013;4(1):3-12.
doi:10.15177/seefor.13-01 .

Kovačević, Branislav, Miladinović, Dragana, Orlović, Saša, Katanić, Marina, Kebert, Marko, Kovinčić, Jovana, "Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro" in South-East European Forestry, 4, no. 1 (2013):3-12,
https://doi.org/10.15177/seefor.13-01 . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Miladinović, Dragana
AU  - Katanić, Marina
AU  - Tomović, Zoran
AU  - Pekeč, Saša
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1277
AB  - The effect of low initial medium pH on shoot and root development of five white poplar (Populus alba L.) genotypes was tested. The shoot height, fresh mass of shoots per jar, dry mass of shoots per jar, number of roots, as well as the length of the longest root were measured and final pH of the media determined, after 35 days of culture in vitro. Three initial pH values of the medium were tested: 3.0, 4.0 and 5.5 as control. Agar solidification at pH 3.0 was not achieved after sterilization in autoclave, but it was successful after sterilizing in a microwave oven. The obtained results indicate that the tested genotypes are able to significantly influence the changes of media pH during culture. The effect of differences among the examined media was significant for biomass accumulation and final media pH. Generally, significantly higher values of fresh and dry shoot mass, shoot height and the longest root length were recorded on a medium with initial pH 3.0 then on a standard medium with pH 5.5.The implications of the obtained results for the improvement of in vitro propagation of white poplars are discussed.
AB  - U istraživanju je testiran efekat niske početne pH vrednosti hranljive podloge na razvoj izbojaka i korena pet genotipova bele domaće topole (Populus alba L.). Nakon 35 dana kultivacije u kulturi in vitro mereni su visina izbojaka, sveža masa izbojaka po teglici, suva masa izbojaka po teglici, broj korenova, dužina najdužeg korena i određena je konačna pH vrednost hranljive podloge. Tri početne vrednosti pH medijuma - 3,0, 4,0 i 5,5- ispitane su kao standardna pH vrednost medijuma (kontrola). Problemi sa očvršćavanjem podloge kod podloga sa niskom početnom pH nakon sterilizacije, prevaziđene su sterilizacijom podloge u mikrotalasnoj pećnici. Dobijeni rezultati ukazuju na to da ispitivani genotipovi imaju mogućnost da utiču na promenu pH podloge tokom uzgoja u kulturi in vitro. Većina ispitivanih genotipova bele topole ostvarila je značajno bolji rast i razvoj izbojka i korenovog sistema, kao i akumulaciju biomase na podlozi sa početnom vrednošću pH. U radu se diskutuje o implikacijama dobijenih rezultata za poboljšanje in vitro razmnožavanja bele topole.
PB  - Univerzitet u Beogradu - Šumarski fakultet, Beograd
T2  - Glasnik Šumarskog fakulteta
T1  - The effect of low initial medium pH on in vitro white poplar growth
T1  - Efekat niske početne pH vrednosti hranljive podloge na rast bele topole in vitro
EP  - 80
IS  - 108
SP  - 67
DO  - 10.2298/GSF1308067K
ER  - 

@article{
author = "Kovačević, Branislav and Miladinović, Dragana and Katanić, Marina and Tomović, Zoran and Pekeč, Saša",
year = "2013",
abstract = "The effect of low initial medium pH on shoot and root development of five white poplar (Populus alba L.) genotypes was tested. The shoot height, fresh mass of shoots per jar, dry mass of shoots per jar, number of roots, as well as the length of the longest root were measured and final pH of the media determined, after 35 days of culture in vitro. Three initial pH values of the medium were tested: 3.0, 4.0 and 5.5 as control. Agar solidification at pH 3.0 was not achieved after sterilization in autoclave, but it was successful after sterilizing in a microwave oven. The obtained results indicate that the tested genotypes are able to significantly influence the changes of media pH during culture. The effect of differences among the examined media was significant for biomass accumulation and final media pH. Generally, significantly higher values of fresh and dry shoot mass, shoot height and the longest root length were recorded on a medium with initial pH 3.0 then on a standard medium with pH 5.5.The implications of the obtained results for the improvement of in vitro propagation of white poplars are discussed., U istraživanju je testiran efekat niske početne pH vrednosti hranljive podloge na razvoj izbojaka i korena pet genotipova bele domaće topole (Populus alba L.). Nakon 35 dana kultivacije u kulturi in vitro mereni su visina izbojaka, sveža masa izbojaka po teglici, suva masa izbojaka po teglici, broj korenova, dužina najdužeg korena i određena je konačna pH vrednost hranljive podloge. Tri početne vrednosti pH medijuma - 3,0, 4,0 i 5,5- ispitane su kao standardna pH vrednost medijuma (kontrola). Problemi sa očvršćavanjem podloge kod podloga sa niskom početnom pH nakon sterilizacije, prevaziđene su sterilizacijom podloge u mikrotalasnoj pećnici. Dobijeni rezultati ukazuju na to da ispitivani genotipovi imaju mogućnost da utiču na promenu pH podloge tokom uzgoja u kulturi in vitro. Većina ispitivanih genotipova bele topole ostvarila je značajno bolji rast i razvoj izbojka i korenovog sistema, kao i akumulaciju biomase na podlozi sa početnom vrednošću pH. U radu se diskutuje o implikacijama dobijenih rezultata za poboljšanje in vitro razmnožavanja bele topole.",
publisher = "Univerzitet u Beogradu - Šumarski fakultet, Beograd",
journal = "Glasnik Šumarskog fakulteta",
title = "The effect of low initial medium pH on in vitro white poplar growth, Efekat niske početne pH vrednosti hranljive podloge na rast bele topole in vitro",
pages = "80-67",
number = "108",
doi = "10.2298/GSF1308067K"
}

Kovačević, B., Miladinović, D., Katanić, M., Tomović, Z.,& Pekeč, S.. (2013). The effect of low initial medium pH on in vitro white poplar growth. in Glasnik Šumarskog fakulteta
Univerzitet u Beogradu - Šumarski fakultet, Beograd.(108), 67-80.
https://doi.org/10.2298/GSF1308067K

Kovačević B, Miladinović D, Katanić M, Tomović Z, Pekeč S. The effect of low initial medium pH on in vitro white poplar growth. in Glasnik Šumarskog fakulteta. 2013;(108):67-80.
doi:10.2298/GSF1308067K .

Kovačević, Branislav, Miladinović, Dragana, Katanić, Marina, Tomović, Zoran, Pekeč, Saša, "The effect of low initial medium pH on in vitro white poplar growth" in Glasnik Šumarskog fakulteta, no. 108 (2013):67-80,
https://doi.org/10.2298/GSF1308067K . .

TY  - CONF
AU  - Miladinović, Dragana
AU  - Kovačević, Branislav
AU  - Dimitrijević, Aleksandra
AU  - Imerovski, Ivana
AU  - Jocić, Siniša
AU  - Cvejić, Sandra
AU  - Miklič, Vladimir
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/2715
AB  - In sunflower breeding, it takes six to eight years to obtain inbred lines that are homozygous. Dihaploid production enables production of completely homozygous material for less than a year thus accelerating breeding process. The aim of our research was to select optimal medium for induction of shoot regeneration from cultivated sunflower anthers. Anthers of 74 cultivated sunflower genotypes in BC, generation of back-crossing were surface sterilized and placed on four different, MS-medium based solid regeneration media, The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analyzed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA and REML. Statistical analysis enabled us to determine optimal medium for anther culture and shoot regeneration induction,
PB  - Paris : International Sunflower Association
C3  - Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012
T1  - Towards dihaploid production in sunflower - Selection of regeneration medium
SP  - 05-VC-16
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2715
ER  - 

@conference{
author = "Miladinović, Dragana and Kovačević, Branislav and Dimitrijević, Aleksandra and Imerovski, Ivana and Jocić, Siniša and Cvejić, Sandra and Miklič, Vladimir",
year = "2012",
abstract = "In sunflower breeding, it takes six to eight years to obtain inbred lines that are homozygous. Dihaploid production enables production of completely homozygous material for less than a year thus accelerating breeding process. The aim of our research was to select optimal medium for induction of shoot regeneration from cultivated sunflower anthers. Anthers of 74 cultivated sunflower genotypes in BC, generation of back-crossing were surface sterilized and placed on four different, MS-medium based solid regeneration media, The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analyzed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA and REML. Statistical analysis enabled us to determine optimal medium for anther culture and shoot regeneration induction,",
publisher = "Paris : International Sunflower Association",
journal = "Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012",
title = "Towards dihaploid production in sunflower - Selection of regeneration medium",
pages = "05-VC-16",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2715"
}

Miladinović, D., Kovačević, B., Dimitrijević, A., Imerovski, I., Jocić, S., Cvejić, S.,& Miklič, V.. (2012). Towards dihaploid production in sunflower - Selection of regeneration medium. in Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012
Paris : International Sunflower Association., 05-VC-16.
https://hdl.handle.net/21.15107/rcub_fiver_2715

Miladinović D, Kovačević B, Dimitrijević A, Imerovski I, Jocić S, Cvejić S, Miklič V. Towards dihaploid production in sunflower - Selection of regeneration medium. in Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012. 2012;:05-VC-16.
https://hdl.handle.net/21.15107/rcub_fiver_2715 .

Miladinović, Dragana, Kovačević, Branislav, Dimitrijević, Aleksandra, Imerovski, Ivana, Jocić, Siniša, Cvejić, Sandra, Miklič, Vladimir, "Towards dihaploid production in sunflower - Selection of regeneration medium" in Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012 (2012):05-VC-16,
https://hdl.handle.net/21.15107/rcub_fiver_2715 .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Miladinović, Dragana
AU  - Orlović, Saša
AU  - Tomović, Zoran
AU  - Rončević, Savo
AU  - Poljakovic-Pajnik, Leopold
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1165
AB  - The influence of CoCl2 on cutting rooting of five eastern cottonwood genotypes was examined. Rooted cuttings were treated with 100 mu M CoCl2 four weeks after the planting and morphological rooting characteristics were measured eight weeks after the experiment establishment. According to LSD-test treatment with cobalt chloride had positive effect on almost all examined characters, but only in number of roots on the basal 5 cm of cutting, number of roots from the 5th to 10th cm of cutting, number of roots on basal 10 cm of cutting, and total number of roots that effect was statistically significant. The obtained results indicate that for some difficult-to-root genotypes the treatment with cobalt could have a positive effect on rooting.
T2  - Propagation of Ornamental Plants
T1  - Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings
EP  - 57
IS  - 1
SP  - 52
VL  - 12
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1165
ER  - 

@article{
author = "Kovačević, Branislav and Miladinović, Dragana and Orlović, Saša and Tomović, Zoran and Rončević, Savo and Poljakovic-Pajnik, Leopold",
year = "2012",
abstract = "The influence of CoCl2 on cutting rooting of five eastern cottonwood genotypes was examined. Rooted cuttings were treated with 100 mu M CoCl2 four weeks after the planting and morphological rooting characteristics were measured eight weeks after the experiment establishment. According to LSD-test treatment with cobalt chloride had positive effect on almost all examined characters, but only in number of roots on the basal 5 cm of cutting, number of roots from the 5th to 10th cm of cutting, number of roots on basal 10 cm of cutting, and total number of roots that effect was statistically significant. The obtained results indicate that for some difficult-to-root genotypes the treatment with cobalt could have a positive effect on rooting.",
journal = "Propagation of Ornamental Plants",
title = "Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings",
pages = "57-52",
number = "1",
volume = "12",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1165"
}

Kovačević, B., Miladinović, D., Orlović, S., Tomović, Z., Rončević, S.,& Poljakovic-Pajnik, L.. (2012). Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings. in Propagation of Ornamental Plants, 12(1), 52-57.
https://hdl.handle.net/21.15107/rcub_fiver_1165

Kovačević B, Miladinović D, Orlović S, Tomović Z, Rončević S, Poljakovic-Pajnik L. Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings. in Propagation of Ornamental Plants. 2012;12(1):52-57.
https://hdl.handle.net/21.15107/rcub_fiver_1165 .

Kovačević, Branislav, Miladinović, Dragana, Orlović, Saša, Tomović, Zoran, Rončević, Savo, Poljakovic-Pajnik, Leopold, "Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings" in Propagation of Ornamental Plants, 12, no. 1 (2012):52-57,
https://hdl.handle.net/21.15107/rcub_fiver_1165 .

TY  - JOUR
AU  - Miladinović, Dragana
AU  - Taški-Ajduković, Ksenija
AU  - Nagl, Nevena
AU  - Kovačević, Branislav
AU  - Balešević-Tubić, Svetlana
AU  - Dušanić, Nenad
AU  - Jocić, Siniša
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/1029
AB  - DNA polymorphism in accessions of Helianthus mollis, Helianthus rigidus and Helianthus tuberosus differing in level of resistance to stem white rot caused by Sclerotinia was evaluated in reactions with two RAPD primers, C04 and C15, selected for their potential linkage with resistance to Sclerotinia. In total, 36 fragments using both primers were generated. All fragments generated by C04 were polymorphic, while 5 fragments out of 11 generated by C15 were monomorphic. Two major clusters were identified on the phenogram generated by UPGMA analysis, one comprising plants of H. mollis 1298, and the other where all other accessions were grouped. In order to check and statistically confirm any significant association of specific fragments either with resistance or susceptibility, contingency coefficient test was done. Out of 36 fragments generated, 26 were significantly associated with resistance/susceptibility. Fragment C04-950 bp was found to be significantly associated to susceptibility, as it was generated only in susceptible accessions, while fragment C04- 1200 bp was found to be associated with the resistance, as it only occurred in H. mollis accessions. The selected markers should be checked and results verified in further studies including higher number of wild sunflower accessions differing in their reaction to stem white rot caused by Sclerotinia.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Helia
T1  - DNA polymorphism of wild sunflower accessions highly susceptible or highly tolerant to white rot on stalk
EP  - 100
IS  - 55
SP  - 91
VL  - 34
DO  - 10.2298/HEL1155091M
ER  - 

@article{
author = "Miladinović, Dragana and Taški-Ajduković, Ksenija and Nagl, Nevena and Kovačević, Branislav and Balešević-Tubić, Svetlana and Dušanić, Nenad and Jocić, Siniša",
year = "2011",
abstract = "DNA polymorphism in accessions of Helianthus mollis, Helianthus rigidus and Helianthus tuberosus differing in level of resistance to stem white rot caused by Sclerotinia was evaluated in reactions with two RAPD primers, C04 and C15, selected for their potential linkage with resistance to Sclerotinia. In total, 36 fragments using both primers were generated. All fragments generated by C04 were polymorphic, while 5 fragments out of 11 generated by C15 were monomorphic. Two major clusters were identified on the phenogram generated by UPGMA analysis, one comprising plants of H. mollis 1298, and the other where all other accessions were grouped. In order to check and statistically confirm any significant association of specific fragments either with resistance or susceptibility, contingency coefficient test was done. Out of 36 fragments generated, 26 were significantly associated with resistance/susceptibility. Fragment C04-950 bp was found to be significantly associated to susceptibility, as it was generated only in susceptible accessions, while fragment C04- 1200 bp was found to be associated with the resistance, as it only occurred in H. mollis accessions. The selected markers should be checked and results verified in further studies including higher number of wild sunflower accessions differing in their reaction to stem white rot caused by Sclerotinia.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Helia",
title = "DNA polymorphism of wild sunflower accessions highly susceptible or highly tolerant to white rot on stalk",
pages = "100-91",
number = "55",
volume = "34",
doi = "10.2298/HEL1155091M"
}

Miladinović, D., Taški-Ajduković, K., Nagl, N., Kovačević, B., Balešević-Tubić, S., Dušanić, N.,& Jocić, S.. (2011). DNA polymorphism of wild sunflower accessions highly susceptible or highly tolerant to white rot on stalk. in Helia
Institut za ratarstvo i povrtarstvo, Novi Sad., 34(55), 91-100.
https://doi.org/10.2298/HEL1155091M

Miladinović D, Taški-Ajduković K, Nagl N, Kovačević B, Balešević-Tubić S, Dušanić N, Jocić S. DNA polymorphism of wild sunflower accessions highly susceptible or highly tolerant to white rot on stalk. in Helia. 2011;34(55):91-100.
doi:10.2298/HEL1155091M .

Miladinović, Dragana, Taški-Ajduković, Ksenija, Nagl, Nevena, Kovačević, Branislav, Balešević-Tubić, Svetlana, Dušanić, Nenad, Jocić, Siniša, "DNA polymorphism of wild sunflower accessions highly susceptible or highly tolerant to white rot on stalk" in Helia, 34, no. 55 (2011):91-100,
https://doi.org/10.2298/HEL1155091M . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Orlović, Saša
AU  - Rončević, Savo
AU  - Miladinović, Dragana
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/907
AB  - In this work the effects of silver ion, 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP) and their interactions on micropropagation of Populus alba cl. LBM, male genotype with fastigiate tree shape were studied. Three concentrations of silver nitrate [(Ag NO3), (0, 1.0 and 2.0 mu M)], two concentrations of NAA (0 and 0.1 mu M) and three concentrations of BAP (0, 0.5 and 2 mu M) were added to solidified Aspen culture (AC) media containing 2.0 mu M of kinetin and 10 g L-1 sucrose. Three traits were examined: percentage rooting, percentage microshoots with new shoots and the ratio of shoot length increment within the first and fourth week and length after the first week. The results showed a significant effect of NAA concentration on all the examined traits. The main effects of all examined factors and the interaction between different concentrations of three growth regulators had a significant effect on rooting and multiplication percentage. The inclusion of NAA in the medium at a concentration of 0.1 mu M stimulated multiplication and suppressed shoot growth and rooting, while BAP had the opposite effect. Silver ions stimulated both shoot multiplication and rooting, thus suggesting that it masked the effects of endogenous inhibitors such as ethylene.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM
EP  - 202
SP  - 197
VL  - 885
DO  - 10.17660/ActaHortic.2010.885.25
ER  - 

@article{
author = "Kovačević, Branislav and Orlović, Saša and Rončević, Savo and Miladinović, Dragana",
year = "2010",
abstract = "In this work the effects of silver ion, 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP) and their interactions on micropropagation of Populus alba cl. LBM, male genotype with fastigiate tree shape were studied. Three concentrations of silver nitrate [(Ag NO3), (0, 1.0 and 2.0 mu M)], two concentrations of NAA (0 and 0.1 mu M) and three concentrations of BAP (0, 0.5 and 2 mu M) were added to solidified Aspen culture (AC) media containing 2.0 mu M of kinetin and 10 g L-1 sucrose. Three traits were examined: percentage rooting, percentage microshoots with new shoots and the ratio of shoot length increment within the first and fourth week and length after the first week. The results showed a significant effect of NAA concentration on all the examined traits. The main effects of all examined factors and the interaction between different concentrations of three growth regulators had a significant effect on rooting and multiplication percentage. The inclusion of NAA in the medium at a concentration of 0.1 mu M stimulated multiplication and suppressed shoot growth and rooting, while BAP had the opposite effect. Silver ions stimulated both shoot multiplication and rooting, thus suggesting that it masked the effects of endogenous inhibitors such as ethylene.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM",
pages = "202-197",
volume = "885",
doi = "10.17660/ActaHortic.2010.885.25"
}

Kovačević, B., Orlović, S., Rončević, S.,& Miladinović, D.. (2010). The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM. in Acta Horticulturae
International Society for Horticultural Science., 885, 197-202.
https://doi.org/10.17660/ActaHortic.2010.885.25

Kovačević B, Orlović S, Rončević S, Miladinović D. The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM. in Acta Horticulturae. 2010;885:197-202.
doi:10.17660/ActaHortic.2010.885.25 .

Kovačević, Branislav, Orlović, Saša, Rončević, Savo, Miladinović, Dragana, "The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM" in Acta Horticulturae, 885 (2010):197-202,
https://doi.org/10.17660/ActaHortic.2010.885.25 . .

TY  - JOUR
AU  - Halmagyi, A.
AU  - Kevrešan, Slavko
AU  - Kovačević, Branislav
AU  - Orlović, Saša
AU  - Miladinović, Dragana
AU  - Ćirin-Novta, Vera
AU  - Kuhajda, Ksenija
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/914
AB  - The study describes the effect of naphthenates and their fractions on rooting of in vitro grown Sequoia sempervirens (Lamb. ex D. Don) Endl. shoots. Natural naphthenic acids have been isolated by alkaline extraction from the middle gas fraction of the crude oil. Sequoia sempervirens shoots (1 cm in length) were grown on Murashige and Skoog (1962) (MS) medium supplemented with either total naphthenate preparation, naphthenate fractions obtained by extraction on different pH (pH 2, pH 4, pH 7 and pH 9), or indole-3-butyric acid (IBA) in different concentrations (twenty treatments tested). The rooting testing was based on number and total length of roots formed after four weeks of in vitro growth. Similarly to total length of roots, the highest number of roots per explant (approximate to 7) was achieved in medium containing 50 mu M of the naphthenate fraction extracted at pH 2 and in medium containing 50 mu M of the fraction extracted at pH 9. That is triple higher than in the control and significantly better than in the best IBA-treatment (50 mu M IBA), where five roots per explants in average were formed. Similar to the results obtained for some agricultural and tree forest species, our results with Sequoia sempervirens confirm the possibility of rooting stimulation by naphthenates.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens
EP  - 144
SP  - 139
VL  - 885
DO  - 10.17660/actahortic.2010.885.18
ER  - 

@article{
author = "Halmagyi, A. and Kevrešan, Slavko and Kovačević, Branislav and Orlović, Saša and Miladinović, Dragana and Ćirin-Novta, Vera and Kuhajda, Ksenija",
year = "2010",
abstract = "The study describes the effect of naphthenates and their fractions on rooting of in vitro grown Sequoia sempervirens (Lamb. ex D. Don) Endl. shoots. Natural naphthenic acids have been isolated by alkaline extraction from the middle gas fraction of the crude oil. Sequoia sempervirens shoots (1 cm in length) were grown on Murashige and Skoog (1962) (MS) medium supplemented with either total naphthenate preparation, naphthenate fractions obtained by extraction on different pH (pH 2, pH 4, pH 7 and pH 9), or indole-3-butyric acid (IBA) in different concentrations (twenty treatments tested). The rooting testing was based on number and total length of roots formed after four weeks of in vitro growth. Similarly to total length of roots, the highest number of roots per explant (approximate to 7) was achieved in medium containing 50 mu M of the naphthenate fraction extracted at pH 2 and in medium containing 50 mu M of the fraction extracted at pH 9. That is triple higher than in the control and significantly better than in the best IBA-treatment (50 mu M IBA), where five roots per explants in average were formed. Similar to the results obtained for some agricultural and tree forest species, our results with Sequoia sempervirens confirm the possibility of rooting stimulation by naphthenates.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens",
pages = "144-139",
volume = "885",
doi = "10.17660/actahortic.2010.885.18"
}

Halmagyi, A., Kevrešan, S., Kovačević, B., Orlović, S., Miladinović, D., Ćirin-Novta, V.,& Kuhajda, K.. (2010). Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens. in Acta Horticulturae
International Society for Horticultural Science., 885, 139-144.
https://doi.org/10.17660/actahortic.2010.885.18

Halmagyi A, Kevrešan S, Kovačević B, Orlović S, Miladinović D, Ćirin-Novta V, Kuhajda K. Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens. in Acta Horticulturae. 2010;885:139-144.
doi:10.17660/actahortic.2010.885.18 .

Halmagyi, A., Kevrešan, Slavko, Kovačević, Branislav, Orlović, Saša, Miladinović, Dragana, Ćirin-Novta, Vera, Kuhajda, Ksenija, "Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens" in Acta Horticulturae, 885 (2010):139-144,
https://doi.org/10.17660/actahortic.2010.885.18 . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Kevrešan, Slavko
AU  - Ćirin-Novta, Vera
AU  - Kuhajda, Ksenija
AU  - Kandrač, Julijan
AU  - Miladinović, Dragana
PY  - 2008
UR  - http://fiver.ifvcns.rs/handle/123456789/567
AB  - Naphtenic acids, by-product in oil refinement, are well known soil pollutants and active substances in preparations for plant and wood protection. The results of multiannual research indicated the possibility of their utilization for stimulation of cutting rooting. Two to three cm high shoots of Populus alba cl. LBM genotype were set for one hour on ACM medium with 4, 16 or 48 μM concentration of indol-3-butyric acid, 1-naphthalene acetic acid or Na-salts of naphthenic acids (for which the effect of 10 and 30 minutes treatment on medium with 48 μM was also examined). Then, treated micro-shoots were transferred on ACM medium without hormones. The differences among treatments were the most distinguishable for rooting percentage after two weeks of cultivation. One-hour treatment on the medium with 16 μM and 30 minutes treatment on medium with 48 μM of Na-naphthenates gave the best results. .
AB  - Naftenske kiseline, nusproizvodi u procesu rafinacije nafte, su poznati zagađivači zemljišta i aktivna materija sredstava za zaštitu biljka i drveta. Rezultati višegodišnjih istraživanja ukazuju i na mogućnost njihove upotrebe kao sredstava za stimulaciju oživljavanja reznica. Dva do tri cm visoki mikroizbojci genotipa Populus alba cl. LBM su jedan čas bili postavljeni na ACM podlozi sa 4, 16 ili 48 μM koncentracijom indolbuterne kiseline, naftilsirćetne kiseline ili Na-soli naftenskih kiselina (kod kojih je ispitan i uticaj 10-to i 30-to minutnog tretmana pri koncentraciji 48 μM). Zatim su mikroizbojci postavljeni na ACM podlogu bez hormona. Razlike među tretmanima su se najviše ispoljile u procentu ožiljavanja nakon dve nedelje kultivacije. Jednočasovni tretmani na podlozi sa 16 μM i polučasovni na podlozi sa 48 μM Na-naftenata su dali najbolje rezultate. .
PB  - Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad
T2  - Topola
T1  - The effect of naphthenic acids on white poplar (Populus alba L.) microcuttings' rooting
T1  - Efekat naftenskih kiselina na ožiljavanje mikroizbojaka bele topole (Populus alba L.)
EP  - 48
IS  - 181-182
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_fiver_567
ER  - 

@article{
author = "Kovačević, Branislav and Kevrešan, Slavko and Ćirin-Novta, Vera and Kuhajda, Ksenija and Kandrač, Julijan and Miladinović, Dragana",
year = "2008",
abstract = "Naphtenic acids, by-product in oil refinement, are well known soil pollutants and active substances in preparations for plant and wood protection. The results of multiannual research indicated the possibility of their utilization for stimulation of cutting rooting. Two to three cm high shoots of Populus alba cl. LBM genotype were set for one hour on ACM medium with 4, 16 or 48 μM concentration of indol-3-butyric acid, 1-naphthalene acetic acid or Na-salts of naphthenic acids (for which the effect of 10 and 30 minutes treatment on medium with 48 μM was also examined). Then, treated micro-shoots were transferred on ACM medium without hormones. The differences among treatments were the most distinguishable for rooting percentage after two weeks of cultivation. One-hour treatment on the medium with 16 μM and 30 minutes treatment on medium with 48 μM of Na-naphthenates gave the best results. ., Naftenske kiseline, nusproizvodi u procesu rafinacije nafte, su poznati zagađivači zemljišta i aktivna materija sredstava za zaštitu biljka i drveta. Rezultati višegodišnjih istraživanja ukazuju i na mogućnost njihove upotrebe kao sredstava za stimulaciju oživljavanja reznica. Dva do tri cm visoki mikroizbojci genotipa Populus alba cl. LBM su jedan čas bili postavljeni na ACM podlozi sa 4, 16 ili 48 μM koncentracijom indolbuterne kiseline, naftilsirćetne kiseline ili Na-soli naftenskih kiselina (kod kojih je ispitan i uticaj 10-to i 30-to minutnog tretmana pri koncentraciji 48 μM). Zatim su mikroizbojci postavljeni na ACM podlogu bez hormona. Razlike među tretmanima su se najviše ispoljile u procentu ožiljavanja nakon dve nedelje kultivacije. Jednočasovni tretmani na podlozi sa 16 μM i polučasovni na podlozi sa 48 μM Na-naftenata su dali najbolje rezultate. .",
publisher = "Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad",
journal = "Topola",
title = "The effect of naphthenic acids on white poplar (Populus alba L.) microcuttings' rooting, Efekat naftenskih kiselina na ožiljavanje mikroizbojaka bele topole (Populus alba L.)",
pages = "48-41",
number = "181-182",
url = "https://hdl.handle.net/21.15107/rcub_fiver_567"
}

Kovačević, B., Kevrešan, S., Ćirin-Novta, V., Kuhajda, K., Kandrač, J.,& Miladinović, D.. (2008). The effect of naphthenic acids on white poplar (Populus alba L.) microcuttings' rooting. in Topola
Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad.(181-182), 41-48.
https://hdl.handle.net/21.15107/rcub_fiver_567

Kovačević B, Kevrešan S, Ćirin-Novta V, Kuhajda K, Kandrač J, Miladinović D. The effect of naphthenic acids on white poplar (Populus alba L.) microcuttings' rooting. in Topola. 2008;(181-182):41-48.
https://hdl.handle.net/21.15107/rcub_fiver_567 .

Kovačević, Branislav, Kevrešan, Slavko, Ćirin-Novta, Vera, Kuhajda, Ksenija, Kandrač, Julijan, Miladinović, Dragana, "The effect of naphthenic acids on white poplar (Populus alba L.) microcuttings' rooting" in Topola, no. 181-182 (2008):41-48,
https://hdl.handle.net/21.15107/rcub_fiver_567 .