Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot
2014
Аутори
Miladinović, DraganaTaški-Ajduković, Ksenija
Nagl, Nevena
Kovačević, Branislav
Dimitrijević, Aleksandra
Imerovski, Ivana
Miladinović, Jegor
Dušanić, Nenad
Balalić, Igor
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism among accessions of wild sunflower species Helianthus maximiliani, Helianthus tuberosus, Helianthus mollis and Helianthus rigidus with different tolerance to mid-stalk white rot and selection of potential markers for different levels of tolerance to this disease. Estimates of genetic variation showed that genetic diversity was equally distributed between Helianthus species and within them. Cluster analysis corresponded to the phylogenetic relations within the genus Helianthus. The results obtained by principal coordinates analysis (PCoA), where the first two principal coordinates accounted for 83.7% of total variation, perfectly coincided with the results of cluster analysis. Contingency coefficient significance test showed that most of the used primers generated bands associated with some level of tolerance or susceptibility to mid-stalk white rot. Furthermore, contingency analysis showed that primer C12 ...generated bands associated with resistance (100%) to mid-stalk white rot both in H. mollis and in all accessions, while primer X18 generated bands significantly associated with high tolerance (75%) in H. rigidus, H. mollis as well as in all tested accessions. The C15-600 bp locus was found to be significantly associated with high tolerance (75%) in all accessions, and medium tolerance (50%) in H. mollis.
Кључне речи:
breeding / contingency test / Helianthus / multivariate analysis / RAPD markers / Sclerotinia sclerotiorumИзвор:
Genetika-Belgrade, 2014, 46, 2, 331-342Издавач:
- Društvo genetičara Srbije, Beograd
Финансирање / пројекти:
- Развој нових сорти и побољшање технологија производње уљаних биљних врста за различите намене (RS-MESTD-Technological Development (TD or TR)-31025)
DOI: 10.2298/GENSR1402331M
ISSN: 0534-0012
WoS: 000342659500001
Scopus: 2-s2.0-84968906660
Колекције
Институција/група
FiVeRTY - JOUR AU - Miladinović, Dragana AU - Taški-Ajduković, Ksenija AU - Nagl, Nevena AU - Kovačević, Branislav AU - Dimitrijević, Aleksandra AU - Imerovski, Ivana AU - Miladinović, Jegor AU - Dušanić, Nenad AU - Balalić, Igor PY - 2014 UR - http://fiver.ifvcns.rs/handle/123456789/1323 AB - Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism among accessions of wild sunflower species Helianthus maximiliani, Helianthus tuberosus, Helianthus mollis and Helianthus rigidus with different tolerance to mid-stalk white rot and selection of potential markers for different levels of tolerance to this disease. Estimates of genetic variation showed that genetic diversity was equally distributed between Helianthus species and within them. Cluster analysis corresponded to the phylogenetic relations within the genus Helianthus. The results obtained by principal coordinates analysis (PCoA), where the first two principal coordinates accounted for 83.7% of total variation, perfectly coincided with the results of cluster analysis. Contingency coefficient significance test showed that most of the used primers generated bands associated with some level of tolerance or susceptibility to mid-stalk white rot. Furthermore, contingency analysis showed that primer C12 generated bands associated with resistance (100%) to mid-stalk white rot both in H. mollis and in all accessions, while primer X18 generated bands significantly associated with high tolerance (75%) in H. rigidus, H. mollis as well as in all tested accessions. The C15-600 bp locus was found to be significantly associated with high tolerance (75%) in all accessions, and medium tolerance (50%) in H. mollis. PB - Društvo genetičara Srbije, Beograd T2 - Genetika-Belgrade T1 - Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot EP - 342 IS - 2 SP - 331 VL - 46 DO - 10.2298/GENSR1402331M ER -
@article{ author = "Miladinović, Dragana and Taški-Ajduković, Ksenija and Nagl, Nevena and Kovačević, Branislav and Dimitrijević, Aleksandra and Imerovski, Ivana and Miladinović, Jegor and Dušanić, Nenad and Balalić, Igor", year = "2014", abstract = "Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism among accessions of wild sunflower species Helianthus maximiliani, Helianthus tuberosus, Helianthus mollis and Helianthus rigidus with different tolerance to mid-stalk white rot and selection of potential markers for different levels of tolerance to this disease. Estimates of genetic variation showed that genetic diversity was equally distributed between Helianthus species and within them. Cluster analysis corresponded to the phylogenetic relations within the genus Helianthus. The results obtained by principal coordinates analysis (PCoA), where the first two principal coordinates accounted for 83.7% of total variation, perfectly coincided with the results of cluster analysis. Contingency coefficient significance test showed that most of the used primers generated bands associated with some level of tolerance or susceptibility to mid-stalk white rot. Furthermore, contingency analysis showed that primer C12 generated bands associated with resistance (100%) to mid-stalk white rot both in H. mollis and in all accessions, while primer X18 generated bands significantly associated with high tolerance (75%) in H. rigidus, H. mollis as well as in all tested accessions. The C15-600 bp locus was found to be significantly associated with high tolerance (75%) in all accessions, and medium tolerance (50%) in H. mollis.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika-Belgrade", title = "Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot", pages = "342-331", number = "2", volume = "46", doi = "10.2298/GENSR1402331M" }
Miladinović, D., Taški-Ajduković, K., Nagl, N., Kovačević, B., Dimitrijević, A., Imerovski, I., Miladinović, J., Dušanić, N.,& Balalić, I.. (2014). Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot. in Genetika-Belgrade Društvo genetičara Srbije, Beograd., 46(2), 331-342. https://doi.org/10.2298/GENSR1402331M
Miladinović D, Taški-Ajduković K, Nagl N, Kovačević B, Dimitrijević A, Imerovski I, Miladinović J, Dušanić N, Balalić I. Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot. in Genetika-Belgrade. 2014;46(2):331-342. doi:10.2298/GENSR1402331M .
Miladinović, Dragana, Taški-Ajduković, Ksenija, Nagl, Nevena, Kovačević, Branislav, Dimitrijević, Aleksandra, Imerovski, Ivana, Miladinović, Jegor, Dušanić, Nenad, Balalić, Igor, "Genetic diversity of wild sunflower (helianthus sp.) Accessions with different tolerance to mid-stalk white rot" in Genetika-Belgrade, 46, no. 2 (2014):331-342, https://doi.org/10.2298/GENSR1402331M . .