Towards dihaploid production in sunflower - Selection of regeneration medium
Аутори
Miladinović, DraganaKovačević, Branislav
Dimitrijević, Aleksandra
Imerovski, Ivana
Jocić, Siniša
Cvejić, Sandra
Miklič, Vladimir
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In sunflower breeding, it takes six to eight years to obtain inbred lines that are homozygous. Dihaploid production enables production of completely homozygous material for less than a year thus accelerating breeding process. The aim of our research was to select optimal medium for induction of shoot regeneration from cultivated sunflower anthers. Anthers of 74 cultivated sunflower genotypes in BC, generation of back-crossing were surface sterilized and placed on four different, MS-medium based solid regeneration media, The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analyzed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA and REML. Statistical analysis enabled us to determine optimal medium for anther culture and shoot regeneration induction,
Кључне речи:
anther culture / dihaploid / regeneration / sunflowerИзвор:
Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012, 2012, 05-VC-16-Издавач:
- Paris : International Sunflower Association
Финансирање / пројекти:
- Развој нових сорти и побољшање технологија производње уљаних биљних врста за различите намене (RS-MESTD-Technological Development (TD or TR)-31025)
Колекције
Институција/група
FiVeRTY - CONF AU - Miladinović, Dragana AU - Kovačević, Branislav AU - Dimitrijević, Aleksandra AU - Imerovski, Ivana AU - Jocić, Siniša AU - Cvejić, Sandra AU - Miklič, Vladimir PY - 2012 UR - http://fiver.ifvcns.rs/handle/123456789/2715 AB - In sunflower breeding, it takes six to eight years to obtain inbred lines that are homozygous. Dihaploid production enables production of completely homozygous material for less than a year thus accelerating breeding process. The aim of our research was to select optimal medium for induction of shoot regeneration from cultivated sunflower anthers. Anthers of 74 cultivated sunflower genotypes in BC, generation of back-crossing were surface sterilized and placed on four different, MS-medium based solid regeneration media, The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analyzed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA and REML. Statistical analysis enabled us to determine optimal medium for anther culture and shoot regeneration induction, PB - Paris : International Sunflower Association C3 - Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012 T1 - Towards dihaploid production in sunflower - Selection of regeneration medium SP - 05-VC-16 UR - https://hdl.handle.net/21.15107/rcub_fiver_2715 ER -
@conference{ author = "Miladinović, Dragana and Kovačević, Branislav and Dimitrijević, Aleksandra and Imerovski, Ivana and Jocić, Siniša and Cvejić, Sandra and Miklič, Vladimir", year = "2012", abstract = "In sunflower breeding, it takes six to eight years to obtain inbred lines that are homozygous. Dihaploid production enables production of completely homozygous material for less than a year thus accelerating breeding process. The aim of our research was to select optimal medium for induction of shoot regeneration from cultivated sunflower anthers. Anthers of 74 cultivated sunflower genotypes in BC, generation of back-crossing were surface sterilized and placed on four different, MS-medium based solid regeneration media, The appearance of organogenesis or somatic embryogenesis was observed and obtained data statistically analyzed. The experiment was set as completely randomised, with two factors. Callus, somatic embryo, shoot and root regeneration on the anthers of the tested genotypes was observed. Data were analysed by ANOVA and REML. Statistical analysis enabled us to determine optimal medium for anther culture and shoot regeneration induction,", publisher = "Paris : International Sunflower Association", journal = "Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012", title = "Towards dihaploid production in sunflower - Selection of regeneration medium", pages = "05-VC-16", url = "https://hdl.handle.net/21.15107/rcub_fiver_2715" }
Miladinović, D., Kovačević, B., Dimitrijević, A., Imerovski, I., Jocić, S., Cvejić, S.,& Miklič, V.. (2012). Towards dihaploid production in sunflower - Selection of regeneration medium. in Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012 Paris : International Sunflower Association., 05-VC-16. https://hdl.handle.net/21.15107/rcub_fiver_2715
Miladinović D, Kovačević B, Dimitrijević A, Imerovski I, Jocić S, Cvejić S, Miklič V. Towards dihaploid production in sunflower - Selection of regeneration medium. in Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012. 2012;:05-VC-16. https://hdl.handle.net/21.15107/rcub_fiver_2715 .
Miladinović, Dragana, Kovačević, Branislav, Dimitrijević, Aleksandra, Imerovski, Ivana, Jocić, Siniša, Cvejić, Sandra, Miklič, Vladimir, "Towards dihaploid production in sunflower - Selection of regeneration medium" in Proceedings, 18th International Sunflower Conference, Mar del Plata & Balcare, Argentina, 27 February - 1 March 2012 (2012):05-VC-16, https://hdl.handle.net/21.15107/rcub_fiver_2715 .