In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra
Апстракт
Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10(-3), 10(-4) and 10(-7) M ( buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10(-7) M Cu2+. As the medium with 10(-3) M Cu2+ exhibited a toxic effect, the effect of 10(-4) M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the appl...ied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.
Кључне речи:
European black poplar / phytoextraction / low pH / tissue culture / microwave sterilizationИзвор:
Archives of Biological Sciences, 2017, 69, 4, 679-687Издавач:
- Srpsko biološko društvo, Beograd, i dr.
Финансирање / пројекти:
- Истраживање климатских промена и њиховог утицаја на животну средину - праћење утицаја, адаптација и ублажавање (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43007)
DOI: 10.2298/ABS170210014V
ISSN: 0354-4664
WoS: 000413353300013
Scopus: 2-s2.0-85031673850
Колекције
Институција/група
FiVeRTY - JOUR AU - Vuksanović, Vanja AU - Kovačević, Branislav AU - Katanić, Marina AU - Orlović, Saša AU - Miladinović, Dragana PY - 2017 UR - http://fiver.ifvcns.rs/handle/123456789/1694 AB - Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10(-3), 10(-4) and 10(-7) M ( buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10(-7) M Cu2+. As the medium with 10(-3) M Cu2+ exhibited a toxic effect, the effect of 10(-4) M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra EP - 687 IS - 4 SP - 679 VL - 69 DO - 10.2298/ABS170210014V ER -
@article{ author = "Vuksanović, Vanja and Kovačević, Branislav and Katanić, Marina and Orlović, Saša and Miladinović, Dragana", year = "2017", abstract = "Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10(-3), 10(-4) and 10(-7) M ( buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10(-7) M Cu2+. As the medium with 10(-3) M Cu2+ exhibited a toxic effect, the effect of 10(-4) M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra", pages = "687-679", number = "4", volume = "69", doi = "10.2298/ABS170210014V" }
Vuksanović, V., Kovačević, B., Katanić, M., Orlović, S.,& Miladinović, D.. (2017). In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 69(4), 679-687. https://doi.org/10.2298/ABS170210014V
Vuksanović V, Kovačević B, Katanić M, Orlović S, Miladinović D. In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. in Archives of Biological Sciences. 2017;69(4):679-687. doi:10.2298/ABS170210014V .
Vuksanović, Vanja, Kovačević, Branislav, Katanić, Marina, Orlović, Saša, Miladinović, Dragana, "In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra" in Archives of Biological Sciences, 69, no. 4 (2017):679-687, https://doi.org/10.2298/ABS170210014V . .