Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed
2018
Аутори
Miladinović, DraganaMiler, Marko
Marjanović-Jeromela, Ana
Imerovski, Ivana
Dimitrijević, Aleksandra
Kovačević, Branislav
Jocić, Siniša
Cvejić, Sandra
Hladni, Nada
Obreht-Vidaković, Dragana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Random amplification of polymorphic DNA (RAPD) analysis was performed on twelve rapeseed genotypes from Institute of Field and Vegetable Crops, Novi Sad, Serbia, genepool in order to identify markers that could be used in marker assisted selection (MAS) for different growing type and selection of the varieties with low or zero level of erucic acid. Out of fifteen RAPD markers, three were monomorphic, whereas twelve had polymorphic profiles. Three primers amplified specific fragments in spring varieties. UBC 25 and UBC 191 amplified the fragments of 450 and 750 bp, respectively, in all tested spring varieties, except in JR-NS-36. Primer UBC 72 generated a fragment of 700 bp that was present in all spring varieties. These fragments were not present in any of winter varieties. None of the tested RAPD primers amplified fragment(s) uniquely present either in varieties with or without (0%) erucic acid or with different erucic acid content. Cluster analysis showed a concordance between the po...sition of varieties in the cluster and their pedigree information, but also enabled separation of spring and winter varieties. Contingency analysis confirmed that fragment UBC 72_700 is specific for spring varieties, while for erucic acid content, only moderate association was found with UBC 137_750.
Кључне речи:
Brassica napus / cluster analysis / contingency analysis / molecular markers / erucic acidИзвор:
Genetika-Belgrade, 2018, 50, 2, 421-430Издавач:
- Društvo genetičara Srbije, Beograd
Финансирање / пројекти:
- Развој нових сорти и побољшање технологија производње уљаних биљних врста за различите намене (RS-MESTD-Technological Development (TD or TR)-31025)
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
DOI: 10.2298/GENSR1802421M
ISSN: 0534-0012
WoS: 000442685800007
Scopus: 2-s2.0-85056223063
Колекције
Институција/група
FiVeRTY - JOUR AU - Miladinović, Dragana AU - Miler, Marko AU - Marjanović-Jeromela, Ana AU - Imerovski, Ivana AU - Dimitrijević, Aleksandra AU - Kovačević, Branislav AU - Jocić, Siniša AU - Cvejić, Sandra AU - Hladni, Nada AU - Obreht-Vidaković, Dragana PY - 2018 UR - http://fiver.ifvcns.rs/handle/123456789/1786 AB - Random amplification of polymorphic DNA (RAPD) analysis was performed on twelve rapeseed genotypes from Institute of Field and Vegetable Crops, Novi Sad, Serbia, genepool in order to identify markers that could be used in marker assisted selection (MAS) for different growing type and selection of the varieties with low or zero level of erucic acid. Out of fifteen RAPD markers, three were monomorphic, whereas twelve had polymorphic profiles. Three primers amplified specific fragments in spring varieties. UBC 25 and UBC 191 amplified the fragments of 450 and 750 bp, respectively, in all tested spring varieties, except in JR-NS-36. Primer UBC 72 generated a fragment of 700 bp that was present in all spring varieties. These fragments were not present in any of winter varieties. None of the tested RAPD primers amplified fragment(s) uniquely present either in varieties with or without (0%) erucic acid or with different erucic acid content. Cluster analysis showed a concordance between the position of varieties in the cluster and their pedigree information, but also enabled separation of spring and winter varieties. Contingency analysis confirmed that fragment UBC 72_700 is specific for spring varieties, while for erucic acid content, only moderate association was found with UBC 137_750. PB - Društvo genetičara Srbije, Beograd T2 - Genetika-Belgrade T1 - Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed EP - 430 IS - 2 SP - 421 VL - 50 DO - 10.2298/GENSR1802421M ER -
@article{ author = "Miladinović, Dragana and Miler, Marko and Marjanović-Jeromela, Ana and Imerovski, Ivana and Dimitrijević, Aleksandra and Kovačević, Branislav and Jocić, Siniša and Cvejić, Sandra and Hladni, Nada and Obreht-Vidaković, Dragana", year = "2018", abstract = "Random amplification of polymorphic DNA (RAPD) analysis was performed on twelve rapeseed genotypes from Institute of Field and Vegetable Crops, Novi Sad, Serbia, genepool in order to identify markers that could be used in marker assisted selection (MAS) for different growing type and selection of the varieties with low or zero level of erucic acid. Out of fifteen RAPD markers, three were monomorphic, whereas twelve had polymorphic profiles. Three primers amplified specific fragments in spring varieties. UBC 25 and UBC 191 amplified the fragments of 450 and 750 bp, respectively, in all tested spring varieties, except in JR-NS-36. Primer UBC 72 generated a fragment of 700 bp that was present in all spring varieties. These fragments were not present in any of winter varieties. None of the tested RAPD primers amplified fragment(s) uniquely present either in varieties with or without (0%) erucic acid or with different erucic acid content. Cluster analysis showed a concordance between the position of varieties in the cluster and their pedigree information, but also enabled separation of spring and winter varieties. Contingency analysis confirmed that fragment UBC 72_700 is specific for spring varieties, while for erucic acid content, only moderate association was found with UBC 137_750.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika-Belgrade", title = "Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed", pages = "430-421", number = "2", volume = "50", doi = "10.2298/GENSR1802421M" }
Miladinović, D., Miler, M., Marjanović-Jeromela, A., Imerovski, I., Dimitrijević, A., Kovačević, B., Jocić, S., Cvejić, S., Hladni, N.,& Obreht-Vidaković, D.. (2018). Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed. in Genetika-Belgrade Društvo genetičara Srbije, Beograd., 50(2), 421-430. https://doi.org/10.2298/GENSR1802421M
Miladinović D, Miler M, Marjanović-Jeromela A, Imerovski I, Dimitrijević A, Kovačević B, Jocić S, Cvejić S, Hladni N, Obreht-Vidaković D. Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed. in Genetika-Belgrade. 2018;50(2):421-430. doi:10.2298/GENSR1802421M .
Miladinović, Dragana, Miler, Marko, Marjanović-Jeromela, Ana, Imerovski, Ivana, Dimitrijević, Aleksandra, Kovačević, Branislav, Jocić, Siniša, Cvejić, Sandra, Hladni, Nada, Obreht-Vidaković, Dragana, "Evaluation of rapd markers as a marker-assisted selection tool for variety type and erucic acid content in rapeseed" in Genetika-Belgrade, 50, no. 2 (2018):421-430, https://doi.org/10.2298/GENSR1802421M . .