TY  - JOUR
AU  - Petrović, Gordana
AU  - Nikolić, Zorica
AU  - Živanović, Tomislav
AU  - Vasiljević, Sanja
AU  - Milošević, Dragana
AU  - Stanisavljević, Nemanja
AU  - Samardžić, Jelena
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4106
AB  - Background: This study was carried out for an understanding of the antioxidant mechanisms of field pea varieties under osmotic stress conditions caused by a lack of water. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APx) and glutathione reductase (GR) were examined and analyzed. The gene expression levels of Cu/Zn SOD, cAPx and GR genes were also examined.
Methods: Osmotic stress was stimulated using PEG 6000 with the osmotic potential of -0.1 MPa in 10 days old plant seedlings. The activity of the antioxidant enzymes was measured in the shoots and roots of pea seedlings. The gene expression levels of genes coding antioxidative enzymes were examined by the reverse transcription polymerase chain reaction (RT-PCR) technique. Arabidopsis 18S rRNA was used as endogenous control.
Result: Osmotic stress changed the activities of antioxidant enzymes in the shoots and roots of pea seedlings. Varieties more tolerant to osmotic stress showed a significant increase in antioxidant activities in shoots and roots, while sensitive varieties showed a significant decrease. The results of the analysis of the expression of genes, coding for antioxidant enzymes, showed that the reaction of the tested cultivars to ROS was the result of increased expression of the tested genes in tolerant cultivars, i.e. decreased expression in sensitive cultivars.
PB  - Agricultural Research Communication Centre
T2  - Legume Research
T1  - Drought-induced Changes in the Antioxidant System in Pisum sativum L.
EP  - 1452
IS  - 11
SP  - 1445
VL  - 46
DO  - 10.18805/LRF-755
ER  - 

@article{
author = "Petrović, Gordana and Nikolić, Zorica and Živanović, Tomislav and Vasiljević, Sanja and Milošević, Dragana and Stanisavljević, Nemanja and Samardžić, Jelena",
year = "2023",
abstract = "Background: This study was carried out for an understanding of the antioxidant mechanisms of field pea varieties under osmotic stress conditions caused by a lack of water. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APx) and glutathione reductase (GR) were examined and analyzed. The gene expression levels of Cu/Zn SOD, cAPx and GR genes were also examined.
Methods: Osmotic stress was stimulated using PEG 6000 with the osmotic potential of -0.1 MPa in 10 days old plant seedlings. The activity of the antioxidant enzymes was measured in the shoots and roots of pea seedlings. The gene expression levels of genes coding antioxidative enzymes were examined by the reverse transcription polymerase chain reaction (RT-PCR) technique. Arabidopsis 18S rRNA was used as endogenous control.
Result: Osmotic stress changed the activities of antioxidant enzymes in the shoots and roots of pea seedlings. Varieties more tolerant to osmotic stress showed a significant increase in antioxidant activities in shoots and roots, while sensitive varieties showed a significant decrease. The results of the analysis of the expression of genes, coding for antioxidant enzymes, showed that the reaction of the tested cultivars to ROS was the result of increased expression of the tested genes in tolerant cultivars, i.e. decreased expression in sensitive cultivars.",
publisher = "Agricultural Research Communication Centre",
journal = "Legume Research",
title = "Drought-induced Changes in the Antioxidant System in Pisum sativum L.",
pages = "1452-1445",
number = "11",
volume = "46",
doi = "10.18805/LRF-755"
}

Petrović, G., Nikolić, Z., Živanović, T., Vasiljević, S., Milošević, D., Stanisavljević, N.,& Samardžić, J.. (2023). Drought-induced Changes in the Antioxidant System in Pisum sativum L.. in Legume Research
Agricultural Research Communication Centre., 46(11), 1445-1452.
https://doi.org/10.18805/LRF-755

Petrović G, Nikolić Z, Živanović T, Vasiljević S, Milošević D, Stanisavljević N, Samardžić J. Drought-induced Changes in the Antioxidant System in Pisum sativum L.. in Legume Research. 2023;46(11):1445-1452.
doi:10.18805/LRF-755 .

Petrović, Gordana, Nikolić, Zorica, Živanović, Tomislav, Vasiljević, Sanja, Milošević, Dragana, Stanisavljević, Nemanja, Samardžić, Jelena, "Drought-induced Changes in the Antioxidant System in Pisum sativum L." in Legume Research, 46, no. 11 (2023):1445-1452,
https://doi.org/10.18805/LRF-755 . .

TY  - CONF
AU  - Jovičić, Dušica
AU  - Ovuka, Jelena
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Marinković, Dragana
AU  - Stojanović, Milan
AU  - Marjanović-Jeromela, Ana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2901
AB  - The effects of physical invigoration seed treatment are manifested in many ways, including morphological and structural aspects, changes in the gene expression and the accumulation of proteins and metabolites. To invigorate seed and to investigate the effects of different invigoration techniques on germination and growth seedling characteristics of sunflower, an experiment was conducted with sunflower hybrid Fantazija at the Laboratory for seed testing, Institute of Field and Vegetable Crops, Novi Sad, Serbia. The process of seed invigoration was performed after seeds were exposed to double stressful condition of high temperature and high humidity (72h at 41°C) (the accelerate aging test) by H2O treatment (the seeds were soaked in water for 24 hours) and KH2PO4  treatment (the seeds were soaked in KH2PO4  solution for 24 hours), followed by washing and drying near to initial weight. Treatments with H2O and KH2PO4  affected the decrease of germination, but they were effective in reducing mean germination time (MGT). After the accelerate aging test, the process of invigoration (both H2O and KH2PO4) influenced a minor increase germination percentage, but MGT was prolonged.
PB  - Paris : International Sunflower Association
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
T1  - The different invigoration techniques for sunflower seeds
EP  - 167
SP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2901
ER  - 

@conference{
author = "Jovičić, Dušica and Ovuka, Jelena and Nikolić, Zorica and Petrović, Gordana and Marinković, Dragana and Stojanović, Milan and Marjanović-Jeromela, Ana",
year = "2022",
abstract = "The effects of physical invigoration seed treatment are manifested in many ways, including morphological and structural aspects, changes in the gene expression and the accumulation of proteins and metabolites. To invigorate seed and to investigate the effects of different invigoration techniques on germination and growth seedling characteristics of sunflower, an experiment was conducted with sunflower hybrid Fantazija at the Laboratory for seed testing, Institute of Field and Vegetable Crops, Novi Sad, Serbia. The process of seed invigoration was performed after seeds were exposed to double stressful condition of high temperature and high humidity (72h at 41°C) (the accelerate aging test) by H2O treatment (the seeds were soaked in water for 24 hours) and KH2PO4  treatment (the seeds were soaked in KH2PO4  solution for 24 hours), followed by washing and drying near to initial weight. Treatments with H2O and KH2PO4  affected the decrease of germination, but they were effective in reducing mean germination time (MGT). After the accelerate aging test, the process of invigoration (both H2O and KH2PO4) influenced a minor increase germination percentage, but MGT was prolonged.",
publisher = "Paris : International Sunflower Association, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia",
title = "The different invigoration techniques for sunflower seeds",
pages = "167-167",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2901"
}

Jovičić, D., Ovuka, J., Nikolić, Z., Petrović, G., Marinković, D., Stojanović, M.,& Marjanović-Jeromela, A.. (2022). The different invigoration techniques for sunflower seeds. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
Paris : International Sunflower Association., 167-167.
https://hdl.handle.net/21.15107/rcub_fiver_2901

Jovičić D, Ovuka J, Nikolić Z, Petrović G, Marinković D, Stojanović M, Marjanović-Jeromela A. The different invigoration techniques for sunflower seeds. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia. 2022;:167-167.
https://hdl.handle.net/21.15107/rcub_fiver_2901 .

Jovičić, Dušica, Ovuka, Jelena, Nikolić, Zorica, Petrović, Gordana, Marinković, Dragana, Stojanović, Milan, Marjanović-Jeromela, Ana, "The different invigoration techniques for sunflower seeds" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia (2022):167-167,
https://hdl.handle.net/21.15107/rcub_fiver_2901 .

TY  - JOUR
AU  - Jovičić, Dušica
AU  - Ovuka, Jelena
AU  - Nikolić, Zorica
AU  - Marinković, Dragana
AU  - Stojanović, Milan
AU  - Tamindžić, Gordana
AU  - Petrović, Gordana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3245
AB  - Seed deterioration is an unavoidable process to which seeds of oilseed plant species such as sunflower are especially sensitive. This study aimed to determine whether different invigoration techniques can improve the attributes of germination in sunflower and alleviate the effects of seed ageing. Both aged and non-aged seeds were subjected to invigoration by pre-soaking with distilled water and with 3% of KH2PO4 for 24 hours at 25°C. Germination performance, seedling growth and SDS-PAGE protein profile were determined. The outcome of invigoration depended on the condition of the seeds: invigoration of seeds with both water and KH2PO4 reduced the percentage of germination in non-aged seeds, while in aged seeds there was an increase in germination compared to the control. The SDS-PAGE seed protein profiles revealed that the low molecular weight proteins produced high-intensity bands and the high molecular weight proteins were in low concentrations. After accelerated ageing followed by H2O treatment, some bands of proteins appeared in the region of 2S albumins and were associated with a higher percentage of germination. Our results point out that invigoration treatments were more effective in low vigour seeds which can provide wide practical benefits.
PB  - International Seed Testing Association
T2  - Seed Science and Technology
T1  - Potential of two hydration treatments for improvement of sunflower seed vigour
EP  - 366
IS  - 3
SP  - 357
VL  - 50
DO  - https://doi.org/10.15258/sst.2022.50.3.07
ER  - 

@article{
author = "Jovičić, Dušica and Ovuka, Jelena and Nikolić, Zorica and Marinković, Dragana and Stojanović, Milan and Tamindžić, Gordana and Petrović, Gordana",
year = "2022",
abstract = "Seed deterioration is an unavoidable process to which seeds of oilseed plant species such as sunflower are especially sensitive. This study aimed to determine whether different invigoration techniques can improve the attributes of germination in sunflower and alleviate the effects of seed ageing. Both aged and non-aged seeds were subjected to invigoration by pre-soaking with distilled water and with 3% of KH2PO4 for 24 hours at 25°C. Germination performance, seedling growth and SDS-PAGE protein profile were determined. The outcome of invigoration depended on the condition of the seeds: invigoration of seeds with both water and KH2PO4 reduced the percentage of germination in non-aged seeds, while in aged seeds there was an increase in germination compared to the control. The SDS-PAGE seed protein profiles revealed that the low molecular weight proteins produced high-intensity bands and the high molecular weight proteins were in low concentrations. After accelerated ageing followed by H2O treatment, some bands of proteins appeared in the region of 2S albumins and were associated with a higher percentage of germination. Our results point out that invigoration treatments were more effective in low vigour seeds which can provide wide practical benefits.",
publisher = "International Seed Testing Association",
journal = "Seed Science and Technology",
title = "Potential of two hydration treatments for improvement of sunflower seed vigour",
pages = "366-357",
number = "3",
volume = "50",
doi = "https://doi.org/10.15258/sst.2022.50.3.07"
}

Jovičić, D., Ovuka, J., Nikolić, Z., Marinković, D., Stojanović, M., Tamindžić, G.,& Petrović, G.. (2022). Potential of two hydration treatments for improvement of sunflower seed vigour. in Seed Science and Technology
International Seed Testing Association., 50(3), 357-366.
https://doi.org/https://doi.org/10.15258/sst.2022.50.3.07

Jovičić D, Ovuka J, Nikolić Z, Marinković D, Stojanović M, Tamindžić G, Petrović G. Potential of two hydration treatments for improvement of sunflower seed vigour. in Seed Science and Technology. 2022;50(3):357-366.
doi:https://doi.org/10.15258/sst.2022.50.3.07 .

Jovičić, Dušica, Ovuka, Jelena, Nikolić, Zorica, Marinković, Dragana, Stojanović, Milan, Tamindžić, Gordana, Petrović, Gordana, "Potential of two hydration treatments for improvement of sunflower seed vigour" in Seed Science and Technology, 50, no. 3 (2022):357-366,
https://doi.org/https://doi.org/10.15258/sst.2022.50.3.07 . .

TY  - CONF
AU  - Nagl, Nevena
AU  - Sinkovič, Lovro
AU  - Vasić, Mirjana
AU  - Meglič, Vladimir
AU  - Savić, Aleksandra
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Pipan, Barbara
AU  - Marjanović-Jeromela, Ana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2804
AB  - Grass pea (Lathyrus sativus L.) is a legume with high protein content and good quantities of essential amino acids. It has high tolerance to drought, high adaptability to extreme conditions, disease resistance and low input requirement for cultivation, which makes it very useful for cultivation by resource-poor farmers. Potential usage of grass pea is limited due to the presence of anti-nutritional factors including protease inhibitors, especially trypsin inhibitors. They have a major impact on nutritional value, by inhibiting pancreatic serine proteases (thus impairing protein digestion), but are also likely to protect legume seed against attack by predators. Although once extensively grown throughout Balkan Peninsula, grass pea was nearly forgotten. In recent years there was an effort to reintroduce and increase the interest of researchers and farmers in this crop. The aim of this study was to establish whether genotype and environment have significant influences on trypsin inhibitor activity (TIA) in grass pea and to determine whether TIA is correlated with seed protein content.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
T1  - Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity
EP  - 82
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2804
ER  - 

@conference{
author = "Nagl, Nevena and Sinkovič, Lovro and Vasić, Mirjana and Meglič, Vladimir and Savić, Aleksandra and Nikolić, Zorica and Petrović, Gordana and Pipan, Barbara and Marjanović-Jeromela, Ana",
year = "2022",
abstract = "Grass pea (Lathyrus sativus L.) is a legume with high protein content and good quantities of essential amino acids. It has high tolerance to drought, high adaptability to extreme conditions, disease resistance and low input requirement for cultivation, which makes it very useful for cultivation by resource-poor farmers. Potential usage of grass pea is limited due to the presence of anti-nutritional factors including protease inhibitors, especially trypsin inhibitors. They have a major impact on nutritional value, by inhibiting pancreatic serine proteases (thus impairing protein digestion), but are also likely to protect legume seed against attack by predators. Although once extensively grown throughout Balkan Peninsula, grass pea was nearly forgotten. In recent years there was an effort to reintroduce and increase the interest of researchers and farmers in this crop. The aim of this study was to establish whether genotype and environment have significant influences on trypsin inhibitor activity (TIA) in grass pea and to determine whether TIA is correlated with seed protein content.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina",
title = "Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2804"
}

Nagl, N., Sinkovič, L., Vasić, M., Meglič, V., Savić, A., Nikolić, Z., Petrović, G., Pipan, B.,& Marjanović-Jeromela, A.. (2022). Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 82-82.
https://hdl.handle.net/21.15107/rcub_fiver_2804

Nagl N, Sinkovič L, Vasić M, Meglič V, Savić A, Nikolić Z, Petrović G, Pipan B, Marjanović-Jeromela A. Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina. 2022;:82-82.
https://hdl.handle.net/21.15107/rcub_fiver_2804 .

Nagl, Nevena, Sinkovič, Lovro, Vasić, Mirjana, Meglič, Vladimir, Savić, Aleksandra, Nikolić, Zorica, Petrović, Gordana, Pipan, Barbara, Marjanović-Jeromela, Ana, "Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity" in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina (2022):82-82,
https://hdl.handle.net/21.15107/rcub_fiver_2804 .

TY  - CONF
AU  - Gvozdenac, Sonja
AU  - Ilić, Aleksandra
AU  - Vasić, Mirjana
AU  - Nagl, Nevena
AU  - Prvulović, Dejan
AU  - Petrović, Gordana
AU  - Tanasković, Snežana
AU  - Vukajlović, Filip
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3143
AB  - Legumes represent a valuable source of proteins in human and animal nutrition, as well as of different nutrients. Also, they enable diversification of agroecosystems and can be considered climate smart crops. Therefore, they are gaining importance in both developed and developing countries. The most important, and often limiting factor in legume production is the presence of the bean weevil (Acanthoscelides obtectus). This work tested the suitability of three different legume species, i. e. common bean, grass pea and faba bean (two accessions of each species) with growing interest in human nutrition, for the development and population growth of this pest, with special emphasis on the levels of anti-nutritive compounds – trypsin inhibitors. The suitability was assessed based on the progeny production after each month. The development and progeny production were significantly affected by the legume species, but also by the accessions. The highest number of emerged adults was on common bean, while the lowest regardless on observation period, was on faba bean, indicating its low suitability for weevil development. Correlation analysis detected significant influence of the level of trypsin inhibitors on progeny production.
PB  - International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Palearctic Regional Section (IOBC-WPRS)
C3  - Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain
T1  - Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?
EP  - 70
SP  - 64
VL  - 159
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3143
ER  - 

@conference{
author = "Gvozdenac, Sonja and Ilić, Aleksandra and Vasić, Mirjana and Nagl, Nevena and Prvulović, Dejan and Petrović, Gordana and Tanasković, Snežana and Vukajlović, Filip",
year = "2022",
abstract = "Legumes represent a valuable source of proteins in human and animal nutrition, as well as of different nutrients. Also, they enable diversification of agroecosystems and can be considered climate smart crops. Therefore, they are gaining importance in both developed and developing countries. The most important, and often limiting factor in legume production is the presence of the bean weevil (Acanthoscelides obtectus). This work tested the suitability of three different legume species, i. e. common bean, grass pea and faba bean (two accessions of each species) with growing interest in human nutrition, for the development and population growth of this pest, with special emphasis on the levels of anti-nutritive compounds – trypsin inhibitors. The suitability was assessed based on the progeny production after each month. The development and progeny production were significantly affected by the legume species, but also by the accessions. The highest number of emerged adults was on common bean, while the lowest regardless on observation period, was on faba bean, indicating its low suitability for weevil development. Correlation analysis detected significant influence of the level of trypsin inhibitors on progeny production.",
publisher = "International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Palearctic Regional Section (IOBC-WPRS)",
journal = "Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain",
title = "Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?",
pages = "70-64",
volume = "159",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3143"
}

Gvozdenac, S., Ilić, A., Vasić, M., Nagl, N., Prvulović, D., Petrović, G., Tanasković, S.,& Vukajlović, F.. (2022). Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?. in Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain
International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Palearctic Regional Section (IOBC-WPRS)., 159, 64-70.
https://hdl.handle.net/21.15107/rcub_fiver_3143

Gvozdenac S, Ilić A, Vasić M, Nagl N, Prvulović D, Petrović G, Tanasković S, Vukajlović F. Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?. in Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain. 2022;159:64-70.
https://hdl.handle.net/21.15107/rcub_fiver_3143 .

Gvozdenac, Sonja, Ilić, Aleksandra, Vasić, Mirjana, Nagl, Nevena, Prvulović, Dejan, Petrović, Gordana, Tanasković, Snežana, Vukajlović, Filip, "Are trypsin inhibitors responsible for the suitability of different legumes for Acanthocelides obtectus development?" in Preceedings, 13th Meeting of the Working Group "Integrated Protection of Stored Products", 3-6 October 2022, Barcelona, Spain, 159 (2022):64-70,
https://hdl.handle.net/21.15107/rcub_fiver_3143 .

TY  - JOUR
AU  - Petrović, Gordana
AU  - Živanović, Tomislav
AU  - Stikić, Radmila
AU  - Nikolić, Zorica
AU  - Jovičić, Dušica
AU  - Tamindžić, Gordana
AU  - Milošević, Dragana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2830
AB  - Germination and seedling growth of seven genotypes of field pea (Pisum sativum L.) were studied in PEG-6000 solution having osmotic potentials -0.1 and -0.2 MPa. A study was undertaken to evaluate the influence of different osmotic potentials (MPa) on seed germination percentage (GP) and mean germination time (MGT). Results show that the percentage of germination decreased with a decrease in osmotic potential, while mean germination time increased. Variety Javor is much more sensitive than the other varieties at all levels of osmotic stress. By contrast, the lowest sensitivity of germination and MGT was found in varieties Mraz and Trezor. Seed germination tests at -0.1 to -0.2 MPa have the potential to be used as tests in field pea. Osmotic stress exposure and its duration significantly affected the growth of seedlings (shoot and root) and the accumulation of biomass, while its effect was more prominent on the growth of the shoot than on root growth, which was also confirmed by the root tolerance index.
AB  - U radu je proučavano klijanje i porast ponika sedam genotipova stočnog graška (Pisum sativum L.) u rastvoru PEG-6000 sa vrednostima osmotskih potencijala od -0,1 i -0,2 MPa. Ispitivanje je izvršeno kako bi se utvrdio uticaj različitih osmotskih potencijala (MPa) na procenat klijavosti semena (GP) i prosečno vreme klijanja (MGT). Rezultati pokazuju da se procenat klijavosti smanjivao uporedo sa smanjenjem osmotskog potencijala, dok se prosečno vreme klijanja povećavalo. Sorta „Javor” mnogo je osetljivija od ostalih sorti pri svim nivoima osmotskog stresa. Suprotno tome, najmanji uticaj na klijanje i MGT utvrđen je kod sorti „Mraz” i „Trezor”. Određivanje klijavosti semena u rastvorima PEG od -0,1 do -0,2 MPa može se koristiti za testiranje stočnog graška. Izloženost osmotskom stresu i njegovo trajanje značajno su uticali na rast ponika (izdanak i koren) i akumulaciju biomase, pri čemu je njegov uticaj bio izraženiji na rast izdanka nego na rast korena, što je potvrdio i indeks tolerantnosti korena.
PB  - Novi Sad : Matica srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Effects of drought stress on germination and seedling growth of different field pea varieties
T1  - Uticaj stresa suše na klijanje i porast ponika različitih genotipova stočnog graška
EP  - 70
SP  - 59
VL  - 140
DO  - 10.2298/ZMSPN2140059P
ER  - 

@article{
author = "Petrović, Gordana and Živanović, Tomislav and Stikić, Radmila and Nikolić, Zorica and Jovičić, Dušica and Tamindžić, Gordana and Milošević, Dragana",
year = "2021",
abstract = "Germination and seedling growth of seven genotypes of field pea (Pisum sativum L.) were studied in PEG-6000 solution having osmotic potentials -0.1 and -0.2 MPa. A study was undertaken to evaluate the influence of different osmotic potentials (MPa) on seed germination percentage (GP) and mean germination time (MGT). Results show that the percentage of germination decreased with a decrease in osmotic potential, while mean germination time increased. Variety Javor is much more sensitive than the other varieties at all levels of osmotic stress. By contrast, the lowest sensitivity of germination and MGT was found in varieties Mraz and Trezor. Seed germination tests at -0.1 to -0.2 MPa have the potential to be used as tests in field pea. Osmotic stress exposure and its duration significantly affected the growth of seedlings (shoot and root) and the accumulation of biomass, while its effect was more prominent on the growth of the shoot than on root growth, which was also confirmed by the root tolerance index., U radu je proučavano klijanje i porast ponika sedam genotipova stočnog graška (Pisum sativum L.) u rastvoru PEG-6000 sa vrednostima osmotskih potencijala od -0,1 i -0,2 MPa. Ispitivanje je izvršeno kako bi se utvrdio uticaj različitih osmotskih potencijala (MPa) na procenat klijavosti semena (GP) i prosečno vreme klijanja (MGT). Rezultati pokazuju da se procenat klijavosti smanjivao uporedo sa smanjenjem osmotskog potencijala, dok se prosečno vreme klijanja povećavalo. Sorta „Javor” mnogo je osetljivija od ostalih sorti pri svim nivoima osmotskog stresa. Suprotno tome, najmanji uticaj na klijanje i MGT utvrđen je kod sorti „Mraz” i „Trezor”. Određivanje klijavosti semena u rastvorima PEG od -0,1 do -0,2 MPa može se koristiti za testiranje stočnog graška. Izloženost osmotskom stresu i njegovo trajanje značajno su uticali na rast ponika (izdanak i koren) i akumulaciju biomase, pri čemu je njegov uticaj bio izraženiji na rast izdanka nego na rast korena, što je potvrdio i indeks tolerantnosti korena.",
publisher = "Novi Sad : Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Effects of drought stress on germination and seedling growth of different field pea varieties, Uticaj stresa suše na klijanje i porast ponika različitih genotipova stočnog graška",
pages = "70-59",
volume = "140",
doi = "10.2298/ZMSPN2140059P"
}

Petrović, G., Živanović, T., Stikić, R., Nikolić, Z., Jovičić, D., Tamindžić, G.,& Milošević, D.. (2021). Effects of drought stress on germination and seedling growth of different field pea varieties. in Zbornik Matice srpske za prirodne nauke
Novi Sad : Matica srpska., 140, 59-70.
https://doi.org/10.2298/ZMSPN2140059P

Petrović G, Živanović T, Stikić R, Nikolić Z, Jovičić D, Tamindžić G, Milošević D. Effects of drought stress on germination and seedling growth of different field pea varieties. in Zbornik Matice srpske za prirodne nauke. 2021;140:59-70.
doi:10.2298/ZMSPN2140059P .

Petrović, Gordana, Živanović, Tomislav, Stikić, Radmila, Nikolić, Zorica, Jovičić, Dušica, Tamindžić, Gordana, Milošević, Dragana, "Effects of drought stress on germination and seedling growth of different field pea varieties" in Zbornik Matice srpske za prirodne nauke, 140 (2021):59-70,
https://doi.org/10.2298/ZMSPN2140059P . .

TY  - JOUR
AU  - Nikolić, Zorica
AU  - Miladinov, Zlatica
AU  - Vasiljević, Sanja
AU  - Katanski, Snežana
AU  - Tamindžić, Gordana
AU  - Milošević, Dragana
AU  - Petrović, Gordana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2512
AB  - Seed vitality and vigour are the two most common parameters related to seed quality. It is crucial to have reliable methods and
tests for seed quality and seed vigour testing. The standard germination test can be used to predict field emergence, but
laboratory seed testing conditions are often in conflict with field conditions. Validated tests for vigour evaluation in legumes are
the conductivity test, the accelerated ageing test and the tetrazolium test. Also, other types of vigour tests have been used to solve
different problems in the seed sector. The modern approach, the computerised image analysis of legume seeds and sprouts, based
on interactive and traditional methods, is a promising alternative for vigour determination.
AB  - Vitalnost semena i vigor su dva najčešća parametra u vezi sa kvalitetom semena. Veoma je važno imati pouzdane metode i testove za ispitivanje kvaliteta i vigora semena. Standardni test klijavosti se može koristiti za predviđanje nicanja u polju, ali uslovi u kojima se nalazi seme tokom ispitivanja često su u suprotnosti sa uslovima na polju. Validovani testovi za procenu vigora leguminoza su test provodljivosti, test ubrzanog starenja i tetrazolijum test. U proučavanju životne sposobnosti semena leguminoza, zavisno od problema koji bi trebalo rešiti, koriste se i drugi vigor testovi. Savremeni pristup, zasnovan na interaktivnim i tradicionalnim metodama mašinskog učenja, omogućava pouzdanu ocenu semena i ponika leguminoza i predstavlja dobru alternativu za objektivno određivanje vigora.
PB  - Kragujevac: Univerzitet u Kragujevcu, Agronomski fakultet u Čačku
T2  - Acta Agriculturae Serbica
T1  - Legume vigour
EP  - 26
IS  - 51
SP  - 19
VL  - 26
DO  - 10.5937/AASer2151019N
ER  - 

@article{
author = "Nikolić, Zorica and Miladinov, Zlatica and Vasiljević, Sanja and Katanski, Snežana and Tamindžić, Gordana and Milošević, Dragana and Petrović, Gordana",
year = "2021",
abstract = "Seed vitality and vigour are the two most common parameters related to seed quality. It is crucial to have reliable methods and
tests for seed quality and seed vigour testing. The standard germination test can be used to predict field emergence, but
laboratory seed testing conditions are often in conflict with field conditions. Validated tests for vigour evaluation in legumes are
the conductivity test, the accelerated ageing test and the tetrazolium test. Also, other types of vigour tests have been used to solve
different problems in the seed sector. The modern approach, the computerised image analysis of legume seeds and sprouts, based
on interactive and traditional methods, is a promising alternative for vigour determination., Vitalnost semena i vigor su dva najčešća parametra u vezi sa kvalitetom semena. Veoma je važno imati pouzdane metode i testove za ispitivanje kvaliteta i vigora semena. Standardni test klijavosti se može koristiti za predviđanje nicanja u polju, ali uslovi u kojima se nalazi seme tokom ispitivanja često su u suprotnosti sa uslovima na polju. Validovani testovi za procenu vigora leguminoza su test provodljivosti, test ubrzanog starenja i tetrazolijum test. U proučavanju životne sposobnosti semena leguminoza, zavisno od problema koji bi trebalo rešiti, koriste se i drugi vigor testovi. Savremeni pristup, zasnovan na interaktivnim i tradicionalnim metodama mašinskog učenja, omogućava pouzdanu ocenu semena i ponika leguminoza i predstavlja dobru alternativu za objektivno određivanje vigora.",
publisher = "Kragujevac: Univerzitet u Kragujevcu, Agronomski fakultet u Čačku",
journal = "Acta Agriculturae Serbica",
title = "Legume vigour",
pages = "26-19",
number = "51",
volume = "26",
doi = "10.5937/AASer2151019N"
}

Nikolić, Z., Miladinov, Z., Vasiljević, S., Katanski, S., Tamindžić, G., Milošević, D.,& Petrović, G.. (2021). Legume vigour. in Acta Agriculturae Serbica
Kragujevac: Univerzitet u Kragujevcu, Agronomski fakultet u Čačku., 26(51), 19-26.
https://doi.org/10.5937/AASer2151019N

Nikolić Z, Miladinov Z, Vasiljević S, Katanski S, Tamindžić G, Milošević D, Petrović G. Legume vigour. in Acta Agriculturae Serbica. 2021;26(51):19-26.
doi:10.5937/AASer2151019N .

Nikolić, Zorica, Miladinov, Zlatica, Vasiljević, Sanja, Katanski, Snežana, Tamindžić, Gordana, Milošević, Dragana, Petrović, Gordana, "Legume vigour" in Acta Agriculturae Serbica, 26, no. 51 (2021):19-26,
https://doi.org/10.5937/AASer2151019N . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Petrović, Gordana
AU  - Vlajić, Slobodan
AU  - Stanković, Ivana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3327
AB  - In the 2018–19 growing season, a total of 51 leaves of eggplant plants grown under field conditions were collected randomly from nine private gardens at four different localities in the Province of Vojvodina. Eggplants with nearly 40% of plants showing bright yellow to white mosaic or mottling of leaves were found throughout the inspected fields (gardens). The collected samples were analyzed for the presence of alfalfa mosaic virus (AMV), cucumber mosaic virus (CMV) and potato virus Y (PVY) using commercial double-antibody sandwich (DAS)-ELISA kits. Serological analysis of eggplant samples revealed the presence of AMV in 80.39% collected samples. None of the analyzed samples was positive for CMV and PVY. The virus was successfully mechanically transmitted to test plants including Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, as well as eggplant seedlings, confirming the infectious nature of the disease. The presence of AMV in eggplants was further verified by reverse transcription–polymerase chain reaction (RT-PCR) and sequencing, using the primers CP AMV1 and CP AMV2 that amplify part of the coat protein (CP) gene. The phylogenetic analysis showed that Serbian AMV isolates grouped into a separate well-supported group together with AMV isolates from Italy, Croatia and previously characterized isolates from Serbia. To our knowledge, this is the first report of AMV infection of eggplant in Serbia.
AB  - Tokom 2018. i 2019. godine, prikupljen je 51 uzorak biljaka plavog patlidžana iz devet useva sa četiri različita lokaliteta gajenja ove biljne vrste u Vojvodini. Tokom pregleda, na blizu 40% biljaka plavog patlidžana mogli su se uočiti simptomi u vidu žućkastobeličastih pega raspoređenih po čitavoj površini liske. Prikupljeni uzorci analizirani su na prisustvo virusa mozaika lucerke (alfalfa mosaic virus, AMV), virusa mozaika krastavca (cucumber mosaic virus, CMV) i Y virusa krompira (potato virus Y, PVY) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Serološkim analizama utvrđeno je prisustvo AMV u 80,39% prikupljenih uzoraka. Prisustvo CMV i PVY nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, kao i na klijance plavog patlidžana, čime je potvrđena infe ktivna priroda oboljenja. Prisustvo AMV u biljkama plavog patlidžana je dalje potvrđeno primenom RT-PCR metode (reverzna transkripcija praćena lančanom reakcijom polimeraze) i sekvencioniranjem odabranih izolata korišćenjem specifičnih prajmera CP AMV1 i CP AMV2, koji omogućavaju umnožavanje gena za protein omotača. Filogenetska analiza pokazala je grupisanje srpskih AMV izolata u jasno odvojenu grupu zajedno sa AMV izolatima iz Italije, Hrvatske i prethodno okarakterisanim izolatima iz Srbije. Prema našim saznanjima, ovo je prvi izveštaj o prisustvu virusa mozaika lucerke na plavom patlidžanu u Srbiji.
PB  - Čačak : Univerzitet u Kragujevcu, Agronomski fakultet u Čačku
T2  - Acta Agriculturae Serbica
T1  - Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia
EP  - 39
IS  - 51
SP  - 33
VL  - 26
DO  - 10.5937/AASer2151033M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Tamindžić, Gordana and Petrović, Gordana and Vlajić, Slobodan and Stanković, Ivana",
year = "2021",
abstract = "In the 2018–19 growing season, a total of 51 leaves of eggplant plants grown under field conditions were collected randomly from nine private gardens at four different localities in the Province of Vojvodina. Eggplants with nearly 40% of plants showing bright yellow to white mosaic or mottling of leaves were found throughout the inspected fields (gardens). The collected samples were analyzed for the presence of alfalfa mosaic virus (AMV), cucumber mosaic virus (CMV) and potato virus Y (PVY) using commercial double-antibody sandwich (DAS)-ELISA kits. Serological analysis of eggplant samples revealed the presence of AMV in 80.39% collected samples. None of the analyzed samples was positive for CMV and PVY. The virus was successfully mechanically transmitted to test plants including Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, as well as eggplant seedlings, confirming the infectious nature of the disease. The presence of AMV in eggplants was further verified by reverse transcription–polymerase chain reaction (RT-PCR) and sequencing, using the primers CP AMV1 and CP AMV2 that amplify part of the coat protein (CP) gene. The phylogenetic analysis showed that Serbian AMV isolates grouped into a separate well-supported group together with AMV isolates from Italy, Croatia and previously characterized isolates from Serbia. To our knowledge, this is the first report of AMV infection of eggplant in Serbia., Tokom 2018. i 2019. godine, prikupljen je 51 uzorak biljaka plavog patlidžana iz devet useva sa četiri različita lokaliteta gajenja ove biljne vrste u Vojvodini. Tokom pregleda, na blizu 40% biljaka plavog patlidžana mogli su se uočiti simptomi u vidu žućkastobeličastih pega raspoređenih po čitavoj površini liske. Prikupljeni uzorci analizirani su na prisustvo virusa mozaika lucerke (alfalfa mosaic virus, AMV), virusa mozaika krastavca (cucumber mosaic virus, CMV) i Y virusa krompira (potato virus Y, PVY) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Serološkim analizama utvrđeno je prisustvo AMV u 80,39% prikupljenih uzoraka. Prisustvo CMV i PVY nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, kao i na klijance plavog patlidžana, čime je potvrđena infe ktivna priroda oboljenja. Prisustvo AMV u biljkama plavog patlidžana je dalje potvrđeno primenom RT-PCR metode (reverzna transkripcija praćena lančanom reakcijom polimeraze) i sekvencioniranjem odabranih izolata korišćenjem specifičnih prajmera CP AMV1 i CP AMV2, koji omogućavaju umnožavanje gena za protein omotača. Filogenetska analiza pokazala je grupisanje srpskih AMV izolata u jasno odvojenu grupu zajedno sa AMV izolatima iz Italije, Hrvatske i prethodno okarakterisanim izolatima iz Srbije. Prema našim saznanjima, ovo je prvi izveštaj o prisustvu virusa mozaika lucerke na plavom patlidžanu u Srbiji.",
publisher = "Čačak : Univerzitet u Kragujevcu, Agronomski fakultet u Čačku",
journal = "Acta Agriculturae Serbica",
title = "Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia",
pages = "39-33",
number = "51",
volume = "26",
doi = "10.5937/AASer2151033M"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Tamindžić, G., Petrović, G., Vlajić, S.,& Stanković, I.. (2021). Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia. in Acta Agriculturae Serbica
Čačak : Univerzitet u Kragujevcu, Agronomski fakultet u Čačku., 26(51), 33-39.
https://doi.org/10.5937/AASer2151033M

Milošević D, Ignjatov M, Nikolić Z, Tamindžić G, Petrović G, Vlajić S, Stanković I. Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia. in Acta Agriculturae Serbica. 2021;26(51):33-39.
doi:10.5937/AASer2151033M .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Tamindžić, Gordana, Petrović, Gordana, Vlajić, Slobodan, Stanković, Ivana, "Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia" in Acta Agriculturae Serbica, 26, no. 51 (2021):33-39,
https://doi.org/10.5937/AASer2151033M . .

TY  - CONF
AU  - Tamindžić, Gordana
AU  - Červenski, Janko
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Vlajić, Slobodan
AU  - Petrović, Gordana
AU  - Ignjatov, Maja
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2331
AB  - Worldwide, agricultural productivity is exposed to increasing constraints in the form of abiotic
stresses such as salinity which affects plant growth and development causing yields loss.
Previous studies suggest that seed priming improves stand establishment under optimal and
stressful environment, given that seed priming might improve seed germination and initial
development by altering seed vigour. This research aimed to define whether various seed priming
treatments could be a feasible strategy to improve garden pea (Pisum sativum L.) resistance to
abiotic stress, namely to salinity. Four garden pea cultivars obtained from the Department for
Vegetable and Alternative Crops, Institute of Field and Vegetable Crops, Novi Sad, Serbia were
included. Seeds of the tested pea cultivars were primed in distilled water (hydropriming), -0.5
MPa polyethylene glycol PEG 6000 solution, and 0.5% KNO3 solution for 24 hours at 25°C in
dark. Non-primed seeds were taken as control. Seeds were submitted to germination test, using
120 mM NaCl to simulate salt stress. The energy of germination, germination, abnormal
seedlings, shoot and root length, fresh and dry shoot and root biomass, and seedling vigour index
were estimated. The results showed significant differences among garden pea cultivars and seed
priming treatments for the assessed traits.
PB  - East Sarajevo : Faculty of Agriculture
C3  - Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021
T1  - Efficiency of hydro- and osmopriming in improving garden pea seed quality and initial plant development under saline stress
EP  - 204
SP  - 204
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2331
ER  - 

@conference{
author = "Tamindžić, Gordana and Červenski, Janko and Milošević, Dragana and Nikolić, Zorica and Vlajić, Slobodan and Petrović, Gordana and Ignjatov, Maja",
year = "2021",
abstract = "Worldwide, agricultural productivity is exposed to increasing constraints in the form of abiotic
stresses such as salinity which affects plant growth and development causing yields loss.
Previous studies suggest that seed priming improves stand establishment under optimal and
stressful environment, given that seed priming might improve seed germination and initial
development by altering seed vigour. This research aimed to define whether various seed priming
treatments could be a feasible strategy to improve garden pea (Pisum sativum L.) resistance to
abiotic stress, namely to salinity. Four garden pea cultivars obtained from the Department for
Vegetable and Alternative Crops, Institute of Field and Vegetable Crops, Novi Sad, Serbia were
included. Seeds of the tested pea cultivars were primed in distilled water (hydropriming), -0.5
MPa polyethylene glycol PEG 6000 solution, and 0.5% KNO3 solution for 24 hours at 25°C in
dark. Non-primed seeds were taken as control. Seeds were submitted to germination test, using
120 mM NaCl to simulate salt stress. The energy of germination, germination, abnormal
seedlings, shoot and root length, fresh and dry shoot and root biomass, and seedling vigour index
were estimated. The results showed significant differences among garden pea cultivars and seed
priming treatments for the assessed traits.",
publisher = "East Sarajevo : Faculty of Agriculture",
journal = "Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021",
title = "Efficiency of hydro- and osmopriming in improving garden pea seed quality and initial plant development under saline stress",
pages = "204-204",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2331"
}

Tamindžić, G., Červenski, J., Milošević, D., Nikolić, Z., Vlajić, S., Petrović, G.,& Ignjatov, M.. (2021). Efficiency of hydro- and osmopriming in improving garden pea seed quality and initial plant development under saline stress. in Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021
East Sarajevo : Faculty of Agriculture., 204-204.
https://hdl.handle.net/21.15107/rcub_fiver_2331

Tamindžić G, Červenski J, Milošević D, Nikolić Z, Vlajić S, Petrović G, Ignjatov M. Efficiency of hydro- and osmopriming in improving garden pea seed quality and initial plant development under saline stress. in Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021. 2021;:204-204.
https://hdl.handle.net/21.15107/rcub_fiver_2331 .

Tamindžić, Gordana, Červenski, Janko, Milošević, Dragana, Nikolić, Zorica, Vlajić, Slobodan, Petrović, Gordana, Ignjatov, Maja, "Efficiency of hydro- and osmopriming in improving garden pea seed quality and initial plant development under saline stress" in Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021 (2021):204-204,
https://hdl.handle.net/21.15107/rcub_fiver_2331 .

TY  - JOUR
AU  - Milošević, Branko
AU  - Mihailović, Vojislav
AU  - Karagić, Đura
AU  - Vasiljević, Sanja
AU  - Milić, Dragan
AU  - Petrović, Gordana
AU  - Katanski, Snežana
AU  - Živanov, Dalibor
AU  - Mikić, Aleksandar
AU  - Đalović, Ivica
AU  - Dolapčev, Anja
AU  - Uhlarik, Ana
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2000
AB  - Field peas are mostly used for animal nutrition in the Balkan region and in the countries of the former USSR, while in the countries of Western Europe, Canada, the USA and the Middle East they are mostly used for human nutrition. Over three years (2010-2012), the yield and grain yield components of the most widespread field pea varieties were examined. The lowest number of plants was determined in 'NS Junior' intended for combined use (65 plants/m2), while 'Dukat' and 'Partner' had 88 and 85 plants/m2 on average, respectively. The average stem height was 158 cm for 'NS Junior', and 65 and 70 cm for 'Dukat' and 'Partner', respectively. 'Dukat' (4846 kg/ha) and 'Partner' (4521 kg/ha) had significantly higher grain yields compared with 'NS Junior' (2717 kg/ha). The content of crude protein in the grain was highest in 'NS Junior' (27.6%), while 'Dukat' and 'Partner' had lower contents (23-25%).
AB  - Proteinski grašak se uglavnom koristi za ishranu životinja u našem regionu i u zemljama bivšeg SSSR-a, dok se u zemljama zapadne Evrope, Kanade, SAD-a i Bliskog istoka najviše koristi za ishranu ljudi. Tokom tri godine (2010-2012) ispitivane su komponente prinosa i prinos zrna najrasprostranjenijih sorti proteinskog graška. Najmanji broj biljaka utvrđen je kod sorte NS Junior, namenjene za kombinovanu upotrebu (65 biljaka / m2), dok su sorte Dukat i Partner imale u proseku 88 i 85 biljaka / m2. Prosečna visina stabljike za sortu NS Junior iznosila je 158 cm, a za sorte Dukat i Partner 65 i 70 cm. Sorte Dukat (4846 kg / ha) i Partner (4521 kg / ha) imale su znatno veći prinos zrna u poređenju sa sortom NS Junior (2717 kg / ha). Sadržaj sirovih proteina u zrnu bio je najveći kod sorte NS Junior (27,6%), dok su sorte Dukat i Partner imale između 23 i 25%.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - Grain yield potential of spring dry pea varieties
EP  - 157
IS  - 50
SP  - 153
VL  - 25
DO  - 10.5937/AASer2050153M
ER  - 

@article{
author = "Milošević, Branko and Mihailović, Vojislav and Karagić, Đura and Vasiljević, Sanja and Milić, Dragan and Petrović, Gordana and Katanski, Snežana and Živanov, Dalibor and Mikić, Aleksandar and Đalović, Ivica and Dolapčev, Anja and Uhlarik, Ana",
year = "2020",
abstract = "Field peas are mostly used for animal nutrition in the Balkan region and in the countries of the former USSR, while in the countries of Western Europe, Canada, the USA and the Middle East they are mostly used for human nutrition. Over three years (2010-2012), the yield and grain yield components of the most widespread field pea varieties were examined. The lowest number of plants was determined in 'NS Junior' intended for combined use (65 plants/m2), while 'Dukat' and 'Partner' had 88 and 85 plants/m2 on average, respectively. The average stem height was 158 cm for 'NS Junior', and 65 and 70 cm for 'Dukat' and 'Partner', respectively. 'Dukat' (4846 kg/ha) and 'Partner' (4521 kg/ha) had significantly higher grain yields compared with 'NS Junior' (2717 kg/ha). The content of crude protein in the grain was highest in 'NS Junior' (27.6%), while 'Dukat' and 'Partner' had lower contents (23-25%)., Proteinski grašak se uglavnom koristi za ishranu životinja u našem regionu i u zemljama bivšeg SSSR-a, dok se u zemljama zapadne Evrope, Kanade, SAD-a i Bliskog istoka najviše koristi za ishranu ljudi. Tokom tri godine (2010-2012) ispitivane su komponente prinosa i prinos zrna najrasprostranjenijih sorti proteinskog graška. Najmanji broj biljaka utvrđen je kod sorte NS Junior, namenjene za kombinovanu upotrebu (65 biljaka / m2), dok su sorte Dukat i Partner imale u proseku 88 i 85 biljaka / m2. Prosečna visina stabljike za sortu NS Junior iznosila je 158 cm, a za sorte Dukat i Partner 65 i 70 cm. Sorte Dukat (4846 kg / ha) i Partner (4521 kg / ha) imale su znatno veći prinos zrna u poređenju sa sortom NS Junior (2717 kg / ha). Sadržaj sirovih proteina u zrnu bio je najveći kod sorte NS Junior (27,6%), dok su sorte Dukat i Partner imale između 23 i 25%.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Grain yield potential of spring dry pea varieties",
pages = "157-153",
number = "50",
volume = "25",
doi = "10.5937/AASer2050153M"
}

Milošević, B., Mihailović, V., Karagić, Đ., Vasiljević, S., Milić, D., Petrović, G., Katanski, S., Živanov, D., Mikić, A., Đalović, I., Dolapčev, A.,& Uhlarik, A.. (2020). Grain yield potential of spring dry pea varieties. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 25(50), 153-157.
https://doi.org/10.5937/AASer2050153M

Milošević B, Mihailović V, Karagić Đ, Vasiljević S, Milić D, Petrović G, Katanski S, Živanov D, Mikić A, Đalović I, Dolapčev A, Uhlarik A. Grain yield potential of spring dry pea varieties. in Acta agriculturae Serbica. 2020;25(50):153-157.
doi:10.5937/AASer2050153M .

Milošević, Branko, Mihailović, Vojislav, Karagić, Đura, Vasiljević, Sanja, Milić, Dragan, Petrović, Gordana, Katanski, Snežana, Živanov, Dalibor, Mikić, Aleksandar, Đalović, Ivica, Dolapčev, Anja, Uhlarik, Ana, "Grain yield potential of spring dry pea varieties" in Acta agriculturae Serbica, 25, no. 50 (2020):153-157,
https://doi.org/10.5937/AASer2050153M . .

TY  - JOUR
AU  - Loc, Marta
AU  - Petrović, Gordana
AU  - Ignjatov, Maja
AU  - Budakov, Dragana
AU  - Petreš, Mladen
AU  - Stojšin, Vera
AU  - Grahovac, Mila
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1960
AB  - World potato production is affected by plant pathogenic bacteria, which significantly limit crop yield and quality in recent years. Bacteria belonging to Pectobacterium and Dickeya genera, causal agents of blackleg and tuber soft rot of potato, are considered as one of the most important pathogens that affect plant production worldwide. Main source of infection are latently infected seed tubers, which transmitted over long trade distances significantly increase disease incidence. Main identification tools for Pectobacterium and Dickeya species until recently were morphological and biochemical characterization methods, followed by pathogenicity tests. Due to phylogenetic heterogeneity of the soft rot and blackleg causing bacteria, identification and differentiation to species and subspecies level became increasingly difficult. Therefore, development of efficient and reliable identification methods is of high importance. Molecular and serological methods offer accurate, reliable and cost-effective differentiation of high specificity and reproducibility. This work aimed to review Pectobacterium i Dickeya species occurring on potato, with special highlight on available molecular identification techniques.
AB  - Proizvodnja krompira u svetu značajno je ugrožena delovanjem fitopatogenih bakterija, te godišnji gubici prinosa poprimaju sve veće razmere. Među deset ekonomski najznačajnijih fitopatogenih bakterija koje smanjuju prinos i kvalitet poljoprivrednih kultura ističu se vrste rodova Pectobacterium i Dickeya - prouzrokovači crne truleži prizemnog dela stabla "crna noga" i vlažne truleži krtola krompira. Najznačajniji izvor infekcije su zaražene semenske krtole, te bakterije ovim putem lako dospevaju u nova područja. Identifikacija bakterija rodova Pectobacterium i Dickeya se do nedavno zasnivala na proučavanju morfoloških odlika bakterijskih kolonija uz upotpunjavanje testovima patogenosti i biohemijskim analizama. Zbog heterogenosti populacije patogena precizna identifikacija prouzrokovača vlažne truleži je dodatno otežana. S obzirom na potrebe za brzom i pouzdanom identifikacijom, u primenu su uvedene serološke i molekularne metode. Molekularne tehnike identifikacije, zbog svoje visoke specifičnosti, brzine izvođenja, pouzdanosti i ponovljivosti danas zauzimaju vodeće mesto u identifikaciji patogena. U radu su prikazane vrste rodova Pectobacterium i Dickeya koje se javljaju na krompiru, sa posebnim osvrtom na molekularne tehnike identifikacije.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot
T1  - Vrste rodova Pectobacterium i Dickeya - prouzrokovači crne truleži prizemnog dela stabla i vlažne truleži krtola krompira
EP  - 66
IS  - 1
SP  - 52
VL  - 48
DO  - 10.5937/BiljLek2001052L
ER  - 

@article{
author = "Loc, Marta and Petrović, Gordana and Ignjatov, Maja and Budakov, Dragana and Petreš, Mladen and Stojšin, Vera and Grahovac, Mila",
year = "2020",
abstract = "World potato production is affected by plant pathogenic bacteria, which significantly limit crop yield and quality in recent years. Bacteria belonging to Pectobacterium and Dickeya genera, causal agents of blackleg and tuber soft rot of potato, are considered as one of the most important pathogens that affect plant production worldwide. Main source of infection are latently infected seed tubers, which transmitted over long trade distances significantly increase disease incidence. Main identification tools for Pectobacterium and Dickeya species until recently were morphological and biochemical characterization methods, followed by pathogenicity tests. Due to phylogenetic heterogeneity of the soft rot and blackleg causing bacteria, identification and differentiation to species and subspecies level became increasingly difficult. Therefore, development of efficient and reliable identification methods is of high importance. Molecular and serological methods offer accurate, reliable and cost-effective differentiation of high specificity and reproducibility. This work aimed to review Pectobacterium i Dickeya species occurring on potato, with special highlight on available molecular identification techniques., Proizvodnja krompira u svetu značajno je ugrožena delovanjem fitopatogenih bakterija, te godišnji gubici prinosa poprimaju sve veće razmere. Među deset ekonomski najznačajnijih fitopatogenih bakterija koje smanjuju prinos i kvalitet poljoprivrednih kultura ističu se vrste rodova Pectobacterium i Dickeya - prouzrokovači crne truleži prizemnog dela stabla "crna noga" i vlažne truleži krtola krompira. Najznačajniji izvor infekcije su zaražene semenske krtole, te bakterije ovim putem lako dospevaju u nova područja. Identifikacija bakterija rodova Pectobacterium i Dickeya se do nedavno zasnivala na proučavanju morfoloških odlika bakterijskih kolonija uz upotpunjavanje testovima patogenosti i biohemijskim analizama. Zbog heterogenosti populacije patogena precizna identifikacija prouzrokovača vlažne truleži je dodatno otežana. S obzirom na potrebe za brzom i pouzdanom identifikacijom, u primenu su uvedene serološke i molekularne metode. Molekularne tehnike identifikacije, zbog svoje visoke specifičnosti, brzine izvođenja, pouzdanosti i ponovljivosti danas zauzimaju vodeće mesto u identifikaciji patogena. U radu su prikazane vrste rodova Pectobacterium i Dickeya koje se javljaju na krompiru, sa posebnim osvrtom na molekularne tehnike identifikacije.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot, Vrste rodova Pectobacterium i Dickeya - prouzrokovači crne truleži prizemnog dela stabla i vlažne truleži krtola krompira",
pages = "66-52",
number = "1",
volume = "48",
doi = "10.5937/BiljLek2001052L"
}

Loc, M., Petrović, G., Ignjatov, M., Budakov, D., Petreš, M., Stojšin, V.,& Grahovac, M.. (2020). Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 48(1), 52-66.
https://doi.org/10.5937/BiljLek2001052L

Loc M, Petrović G, Ignjatov M, Budakov D, Petreš M, Stojšin V, Grahovac M. Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot. in Biljni lekar. 2020;48(1):52-66.
doi:10.5937/BiljLek2001052L .

Loc, Marta, Petrović, Gordana, Ignjatov, Maja, Budakov, Dragana, Petreš, Mladen, Stojšin, Vera, Grahovac, Mila, "Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot" in Biljni lekar, 48, no. 1 (2020):52-66,
https://doi.org/10.5937/BiljLek2001052L . .

TY  - CONF
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Sikora, Vladimir
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Petrović, Gordana
AU  - Tamindžić, Gordana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3058
AB  - The seed vigour can be explained and described as the theoretical capacity of the seed which allows it to accomplish its substantial function under different environmental conditions. The key purpose of testing seed vigour as an underlying physiological seed characteristic is to establish more susceptible and precise seed quality parameters than a germination test. In seeds of oilseed species such as hemp seed (Cannabis sativa), the vigour can be a limiting factor due to the low chemical stability of the lipids compared to starch. The aim of this study was to evaluate the vigour of hemp seed and to make the difference between the tested hemp varieties in terms of vigour capacity. In addition to that, the aim was to identify and standardize the methods for assessing seed vigour and storability of hemp seed. The experimental materials include five varieties of hemp (Helena, Bacalmas, Hu×Uso, Monoica×Uso, Monoica) obtained from the Institute of Field and Vegetable Crops.
PB  - Belgrade : Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
T1  - Assessment of hemp seed vigour
EP  - 197
SP  - 197
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3058
ER  - 

@conference{
author = "Jovičić, Dušica and Nikolić, Zorica and Sikora, Vladimir and Ignjatov, Maja and Milošević, Dragana and Petrović, Gordana and Tamindžić, Gordana",
year = "2019",
abstract = "The seed vigour can be explained and described as the theoretical capacity of the seed which allows it to accomplish its substantial function under different environmental conditions. The key purpose of testing seed vigour as an underlying physiological seed characteristic is to establish more susceptible and precise seed quality parameters than a germination test. In seeds of oilseed species such as hemp seed (Cannabis sativa), the vigour can be a limiting factor due to the low chemical stability of the lipids compared to starch. The aim of this study was to evaluate the vigour of hemp seed and to make the difference between the tested hemp varieties in terms of vigour capacity. In addition to that, the aim was to identify and standardize the methods for assessing seed vigour and storability of hemp seed. The experimental materials include five varieties of hemp (Helena, Bacalmas, Hu×Uso, Monoica×Uso, Monoica) obtained from the Institute of Field and Vegetable Crops.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja",
title = "Assessment of hemp seed vigour",
pages = "197-197",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3058"
}

Jovičić, D., Nikolić, Z., Sikora, V., Ignjatov, M., Milošević, D., Petrović, G.,& Tamindžić, G.. (2019). Assessment of hemp seed vigour. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
Belgrade : Serbian Genetic Society., 197-197.
https://hdl.handle.net/21.15107/rcub_fiver_3058

Jovičić D, Nikolić Z, Sikora V, Ignjatov M, Milošević D, Petrović G, Tamindžić G. Assessment of hemp seed vigour. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:197-197.
https://hdl.handle.net/21.15107/rcub_fiver_3058 .

Jovičić, Dušica, Nikolić, Zorica, Sikora, Vladimir, Ignjatov, Maja, Milošević, Dragana, Petrović, Gordana, Tamindžić, Gordana, "Assessment of hemp seed vigour" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):197-197,
https://hdl.handle.net/21.15107/rcub_fiver_3058 .

TY  - JOUR
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Jovičić, Dušica
AU  - Tamindžić, Gordana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1874
AB  - Global resources are being invested heavily for research and development involving new genetic modification technologies. Growing genetically modified (GM) crops has opened up a debate on the safety of GM crops and the potential long-term harmful effects on the environment, human health and sustainability of new technologies. Food that contains some of the GM ingredients is progressively present on the world market. National legislations differ in countries around the world, from the complete ban on GM foods, to propaganda and acceptance without marking. In our country, according to current regulations, import raw materials for the food industry are controlled for the presence of genetic modification. In order to protect consumers and food safety, it is necessary to monitor the presence of genetic modification at key points in production, from primary production, through processing to market placement.
AB  - Za istraživanja i razvoj novih tehnologija proizvodnje genetičkih modifikacija se na globalnom nivou ulažu ogromna sredstva. Gajenje genetički modifikovanih (GM) biljaka otvorilo je veliku debatu o bezbednosti GM useva i mogućih dugoročnih štetnih uticaja na životnu sredinu, zdravlje ljudi i održivost novih tehnologija. Hrana koja sadrži neki od sastojaka koji je GM je sve više prisutna na svetskom tržištu. Nacionalna zakonodavstva se razlikuju u zemljama u svetu, od potpune zabrane GM hrane, do propagiranja i prihvatanja bez obeležavanja. U našoj zemlji se, prema važećim propisima, uvozne sirovine za prehrambenu industriju kontrolišu na prisustvo genetičke modifikacije. U cilju zaštite potrošača i bezbednosti hrane neophodan je monitoring na prisustvo genetičke modifikacije na ključnim tačkama u proizvodnji, od primarne proizvodnje, preko prerade do plasmana na tržištu.
PB  - Društvo za ishranu Srbije, Beograd
T2  - Hrana i ishrana
T1  - Genetically modified crops and food
T1  - Genetički modifikovani usevi i hrana
EP  - 4
IS  - 1
SP  - 1
VL  - 60
DO  - 10.5937/hraIsh1901001N
ER  - 

@article{
author = "Nikolić, Zorica and Petrović, Gordana and Ignjatov, Maja and Milošević, Dragana and Jovičić, Dušica and Tamindžić, Gordana",
year = "2019",
abstract = "Global resources are being invested heavily for research and development involving new genetic modification technologies. Growing genetically modified (GM) crops has opened up a debate on the safety of GM crops and the potential long-term harmful effects on the environment, human health and sustainability of new technologies. Food that contains some of the GM ingredients is progressively present on the world market. National legislations differ in countries around the world, from the complete ban on GM foods, to propaganda and acceptance without marking. In our country, according to current regulations, import raw materials for the food industry are controlled for the presence of genetic modification. In order to protect consumers and food safety, it is necessary to monitor the presence of genetic modification at key points in production, from primary production, through processing to market placement., Za istraživanja i razvoj novih tehnologija proizvodnje genetičkih modifikacija se na globalnom nivou ulažu ogromna sredstva. Gajenje genetički modifikovanih (GM) biljaka otvorilo je veliku debatu o bezbednosti GM useva i mogućih dugoročnih štetnih uticaja na životnu sredinu, zdravlje ljudi i održivost novih tehnologija. Hrana koja sadrži neki od sastojaka koji je GM je sve više prisutna na svetskom tržištu. Nacionalna zakonodavstva se razlikuju u zemljama u svetu, od potpune zabrane GM hrane, do propagiranja i prihvatanja bez obeležavanja. U našoj zemlji se, prema važećim propisima, uvozne sirovine za prehrambenu industriju kontrolišu na prisustvo genetičke modifikacije. U cilju zaštite potrošača i bezbednosti hrane neophodan je monitoring na prisustvo genetičke modifikacije na ključnim tačkama u proizvodnji, od primarne proizvodnje, preko prerade do plasmana na tržištu.",
publisher = "Društvo za ishranu Srbije, Beograd",
journal = "Hrana i ishrana",
title = "Genetically modified crops and food, Genetički modifikovani usevi i hrana",
pages = "4-1",
number = "1",
volume = "60",
doi = "10.5937/hraIsh1901001N"
}

Nikolić, Z., Petrović, G., Ignjatov, M., Milošević, D., Jovičić, D.,& Tamindžić, G.. (2019). Genetically modified crops and food. in Hrana i ishrana
Društvo za ishranu Srbije, Beograd., 60(1), 1-4.
https://doi.org/10.5937/hraIsh1901001N

Nikolić Z, Petrović G, Ignjatov M, Milošević D, Jovičić D, Tamindžić G. Genetically modified crops and food. in Hrana i ishrana. 2019;60(1):1-4.
doi:10.5937/hraIsh1901001N .

Nikolić, Zorica, Petrović, Gordana, Ignjatov, Maja, Milošević, Dragana, Jovičić, Dušica, Tamindžić, Gordana, "Genetically modified crops and food" in Hrana i ishrana, 60, no. 1 (2019):1-4,
https://doi.org/10.5937/hraIsh1901001N . .

TY  - JOUR
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Sikora, Vladimir
AU  - Tamindžić, Gordana
AU  - Petrović, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1899
AB  - The most critical aspect of plant survival and reproduction is seed germination. Considering the specificity of the Cannabis sativa production, obtaining high-quality hemp seed is not an easy task. Germination testing is often the most reliable way of assessing viability, but the selection of appropriate methods is complex. This study aimed to evaluate the effect of different conditions (i.e. various methods of germination testing) on the successful process of hemp seed germination, achieving maximum germination potential. Our results showed that C. sativa seeds germinated well in different methods, such as between filter papers, top of filter papers and sand, but there were significant differences between the applied methods. This means that the hemp seeds may have different requirements for germination than conditions covered by the Rules recommended methods. Further, the results show that hemp seed may have certain residual dormancy that needs to be investigated in detail.
AB  - Najkritičnija faza preživljavanja i razmnožavanja biljaka je klijanje semena. S obzirom na specifičnost proizvodnje industrijske konoplje (Cannabis sativa), dobijanje visokokvalitetnog semena konoplje nije jednostavno. Ispitivanje klijavosti je često najpouzdaniji način procene vitalnosti, ali je odabir odgovarajućih metoda složen. Ovo istraživanje je sprovedeno kako bi se procenio efekat različitih uslova tj. različitih metoda testiranja klijavosti, na proces klijanja semena konoplje uz postizanje maksimalnog potencijala za klijanje. Dobijeni rezultati pokazuju da seme C. sativa dobro klija primenom različitih metoda (između filter papira, na filter papiru, pesak), ali postoje značajne razlike između primenjenih metoda. Ovo praktično znači da seme konoplje može imati zahteve za klijavost različite od onih koje su obuhvaćene preporučenim metodama. Takođe, rezultati pokazuju da kod semena konoplje možda postoji neki vid zaostale dormantnosti što je potrebno dodatno istražiti.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Comparison of methods for germination testing of Cannabis sativa seed
T1  - Poređenje metoda za ispitivanje klijavosti semena Cannabis sativa
EP  - 75
IS  - 3
SP  - 71
VL  - 56
DO  - 10.5937/ratpov56-21105
ER  - 

@article{
author = "Jovičić, Dušica and Nikolić, Zorica and Sikora, Vladimir and Tamindžić, Gordana and Petrović, Gordana and Ignjatov, Maja and Milošević, Dragana",
year = "2019",
abstract = "The most critical aspect of plant survival and reproduction is seed germination. Considering the specificity of the Cannabis sativa production, obtaining high-quality hemp seed is not an easy task. Germination testing is often the most reliable way of assessing viability, but the selection of appropriate methods is complex. This study aimed to evaluate the effect of different conditions (i.e. various methods of germination testing) on the successful process of hemp seed germination, achieving maximum germination potential. Our results showed that C. sativa seeds germinated well in different methods, such as between filter papers, top of filter papers and sand, but there were significant differences between the applied methods. This means that the hemp seeds may have different requirements for germination than conditions covered by the Rules recommended methods. Further, the results show that hemp seed may have certain residual dormancy that needs to be investigated in detail., Najkritičnija faza preživljavanja i razmnožavanja biljaka je klijanje semena. S obzirom na specifičnost proizvodnje industrijske konoplje (Cannabis sativa), dobijanje visokokvalitetnog semena konoplje nije jednostavno. Ispitivanje klijavosti je često najpouzdaniji način procene vitalnosti, ali je odabir odgovarajućih metoda složen. Ovo istraživanje je sprovedeno kako bi se procenio efekat različitih uslova tj. različitih metoda testiranja klijavosti, na proces klijanja semena konoplje uz postizanje maksimalnog potencijala za klijanje. Dobijeni rezultati pokazuju da seme C. sativa dobro klija primenom različitih metoda (između filter papira, na filter papiru, pesak), ali postoje značajne razlike između primenjenih metoda. Ovo praktično znači da seme konoplje može imati zahteve za klijavost različite od onih koje su obuhvaćene preporučenim metodama. Takođe, rezultati pokazuju da kod semena konoplje možda postoji neki vid zaostale dormantnosti što je potrebno dodatno istražiti.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Comparison of methods for germination testing of Cannabis sativa seed, Poređenje metoda za ispitivanje klijavosti semena Cannabis sativa",
pages = "75-71",
number = "3",
volume = "56",
doi = "10.5937/ratpov56-21105"
}

Jovičić, D., Nikolić, Z., Sikora, V., Tamindžić, G., Petrović, G., Ignjatov, M.,& Milošević, D.. (2019). Comparison of methods for germination testing of Cannabis sativa seed. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 56(3), 71-75.
https://doi.org/10.5937/ratpov56-21105

Jovičić D, Nikolić Z, Sikora V, Tamindžić G, Petrović G, Ignjatov M, Milošević D. Comparison of methods for germination testing of Cannabis sativa seed. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2019;56(3):71-75.
doi:10.5937/ratpov56-21105 .

Jovičić, Dušica, Nikolić, Zorica, Sikora, Vladimir, Tamindžić, Gordana, Petrović, Gordana, Ignjatov, Maja, Milošević, Dragana, "Comparison of methods for germination testing of Cannabis sativa seed" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 56, no. 3 (2019):71-75,
https://doi.org/10.5937/ratpov56-21105 . .

TY  - CONF
AU  - Jovičić, Dušica
AU  - Nikolić, Zorica
AU  - Mikić, Aleksandar
AU  - Petrović, Gordana
AU  - Tamindžić, Gordana
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3330
AB  - Salinity affects almost every aspect of the physiology and biochemistry of plants, including the
whole plant and at the cellular level. There is a significant variation in salt tolerance within vetch
species, providing opportunities for improving salt-stress tolerance using genetic resources.
Considering the significance of successful seed germination and seedling emergence for further
plant establishment, plant development and achieving high crop yields, the study of the interaction
between salt stress and germination is of great importance. The aim of this experiment was to
evaluate the seed vigour of vetch species (Vicia sativa, V. pannonica), through vigour tests under
saline conditions. Germination and vigour were assessed through the standard test, accelerated
ageing test and cold test, estimating the germination percentage, root and shoot length, and total fresh and dry matter accumulation, under three levels of salt stress (100 mM, 150 mM,200 mM NaCl). The obtained results of the experiment showed significant differences between the two species in response to salinity stress. Furthermore, a species that has a higher vigour has shown noticeably higher tolerance to stress. After the accelerated ageing test, V pannonica showed significantly lower values in all the tested parameters, at all concentrations of NaCl.
PB  - Zurich : International Seed Testing Association
C3  - ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019
T1  - Vetch seed vigour evaluation under saline conditions
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3330
ER  - 

@conference{
author = "Jovičić, Dušica and Nikolić, Zorica and Mikić, Aleksandar and Petrović, Gordana and Tamindžić, Gordana and Milošević, Dragana and Ignjatov, Maja",
year = "2019",
abstract = "Salinity affects almost every aspect of the physiology and biochemistry of plants, including the
whole plant and at the cellular level. There is a significant variation in salt tolerance within vetch
species, providing opportunities for improving salt-stress tolerance using genetic resources.
Considering the significance of successful seed germination and seedling emergence for further
plant establishment, plant development and achieving high crop yields, the study of the interaction
between salt stress and germination is of great importance. The aim of this experiment was to
evaluate the seed vigour of vetch species (Vicia sativa, V. pannonica), through vigour tests under
saline conditions. Germination and vigour were assessed through the standard test, accelerated
ageing test and cold test, estimating the germination percentage, root and shoot length, and total fresh and dry matter accumulation, under three levels of salt stress (100 mM, 150 mM,200 mM NaCl). The obtained results of the experiment showed significant differences between the two species in response to salinity stress. Furthermore, a species that has a higher vigour has shown noticeably higher tolerance to stress. After the accelerated ageing test, V pannonica showed significantly lower values in all the tested parameters, at all concentrations of NaCl.",
publisher = "Zurich : International Seed Testing Association",
journal = "ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019",
title = "Vetch seed vigour evaluation under saline conditions",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3330"
}

Jovičić, D., Nikolić, Z., Mikić, A., Petrović, G., Tamindžić, G., Milošević, D.,& Ignjatov, M.. (2019). Vetch seed vigour evaluation under saline conditions. in ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019
Zurich : International Seed Testing Association., 48.
https://hdl.handle.net/21.15107/rcub_fiver_3330

Jovičić D, Nikolić Z, Mikić A, Petrović G, Tamindžić G, Milošević D, Ignjatov M. Vetch seed vigour evaluation under saline conditions. in ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019. 2019;:48.
https://hdl.handle.net/21.15107/rcub_fiver_3330 .

Jovičić, Dušica, Nikolić, Zorica, Mikić, Aleksandar, Petrović, Gordana, Tamindžić, Gordana, Milošević, Dragana, Ignjatov, Maja, "Vetch seed vigour evaluation under saline conditions" in ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019 (2019):48,
https://hdl.handle.net/21.15107/rcub_fiver_3330 .

TY  - CONF
AU  - Nikolić, Zorica
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Jovičić, Dušica
AU  - Petrović, Gordana
AU  - Tamindžić, Gordana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3331
AB  - Genetic markers have been used at the Institute of Field and Vegetable Crops Novi Sad (tFVCNS)
for many years, both for seed quality control and for research purposes. The first laboratory in
Serbia that started applying the electrophoresis of isoenzymes was at the IFVCNS, in 1986. The
distinct advantage of laboratory seed purity control is that the seed can be inspected until the
next sowing at different stages, before harvesting or ripening, during or after seed processing. In
addition to conventional laboratory methods in identifying pathogens, serological and molecular
methods are used nowadays. The main feature of these methods is that they are fast, reliable and
suitable for analysing a large number of samples. Determination of the presence and distribution
of viral and bacterial diseases of paprika (Capsicum annuum L.) were performed using serological
and molecular detection. ln addition to determining the presence and distribution of pepper virus,
identification of the most important and most frequent virus isolates was performed using the
reverse transcription method followed by a polymerase chain reaction (RT-PCR). Differentiation
of Xanthomonas spp., the causative agent of the bacterial pungency. of pepper, was carried
out using restriction fragment length polymorphism (RFLP) analysis of the hrp gene (HrpB
region) and the PCR method, thus confirming the similarity of studied strains with the species
X. euvesicatona. According to the valid legislation in Serbia, commercial cultivation of genetically
modified organism (GMO) crops is not allowed. The laboratories involved in GMO analyses have
been accredited according to ISO I IEC 17025:2006 standard and ISTA standards, and authorised
by the Ministry of Agriculture, Forestry and Water Management for analysis of seeds and product
consignments of plant origin from import. All laboratories use internationally recognised methods
and participate in testing the expertise for GMO analysis in inter-laboratory comparisons. Our
laboratory for seed testing has conducted several studies in order to demonstrate the presence
and quantity of Roundup Ready soybean in crude oil, assess DNA degradation, DNA amplification,
and GMO quantification during tofu production, to report on the validity of labels on imported food
samples from different countries and uptake of GM food derived from soybean into the food chain
in Serbia.
PB  - Zurich : International Seed Testing Association
C3  - ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019
T1  - Genetic markers in seed quality control in Serbia
SP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3331
ER  - 

@conference{
author = "Nikolić, Zorica and Ignjatov, Maja and Milošević, Dragana and Jovičić, Dušica and Petrović, Gordana and Tamindžić, Gordana",
year = "2019",
abstract = "Genetic markers have been used at the Institute of Field and Vegetable Crops Novi Sad (tFVCNS)
for many years, both for seed quality control and for research purposes. The first laboratory in
Serbia that started applying the electrophoresis of isoenzymes was at the IFVCNS, in 1986. The
distinct advantage of laboratory seed purity control is that the seed can be inspected until the
next sowing at different stages, before harvesting or ripening, during or after seed processing. In
addition to conventional laboratory methods in identifying pathogens, serological and molecular
methods are used nowadays. The main feature of these methods is that they are fast, reliable and
suitable for analysing a large number of samples. Determination of the presence and distribution
of viral and bacterial diseases of paprika (Capsicum annuum L.) were performed using serological
and molecular detection. ln addition to determining the presence and distribution of pepper virus,
identification of the most important and most frequent virus isolates was performed using the
reverse transcription method followed by a polymerase chain reaction (RT-PCR). Differentiation
of Xanthomonas spp., the causative agent of the bacterial pungency. of pepper, was carried
out using restriction fragment length polymorphism (RFLP) analysis of the hrp gene (HrpB
region) and the PCR method, thus confirming the similarity of studied strains with the species
X. euvesicatona. According to the valid legislation in Serbia, commercial cultivation of genetically
modified organism (GMO) crops is not allowed. The laboratories involved in GMO analyses have
been accredited according to ISO I IEC 17025:2006 standard and ISTA standards, and authorised
by the Ministry of Agriculture, Forestry and Water Management for analysis of seeds and product
consignments of plant origin from import. All laboratories use internationally recognised methods
and participate in testing the expertise for GMO analysis in inter-laboratory comparisons. Our
laboratory for seed testing has conducted several studies in order to demonstrate the presence
and quantity of Roundup Ready soybean in crude oil, assess DNA degradation, DNA amplification,
and GMO quantification during tofu production, to report on the validity of labels on imported food
samples from different countries and uptake of GM food derived from soybean into the food chain
in Serbia.",
publisher = "Zurich : International Seed Testing Association",
journal = "ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019",
title = "Genetic markers in seed quality control in Serbia",
pages = "62",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3331"
}

Nikolić, Z., Ignjatov, M., Milošević, D., Jovičić, D., Petrović, G.,& Tamindžić, G.. (2019). Genetic markers in seed quality control in Serbia. in ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019
Zurich : International Seed Testing Association., 62.
https://hdl.handle.net/21.15107/rcub_fiver_3331

Nikolić Z, Ignjatov M, Milošević D, Jovičić D, Petrović G, Tamindžić G. Genetic markers in seed quality control in Serbia. in ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019. 2019;:62.
https://hdl.handle.net/21.15107/rcub_fiver_3331 .

Nikolić, Zorica, Ignjatov, Maja, Milošević, Dragana, Jovičić, Dušica, Petrović, Gordana, Tamindžić, Gordana, "Genetic markers in seed quality control in Serbia" in ISTA Seed Symposium Abstracts, 32 ISTA Congress, Hyderabad, India, 26-28 June 2019 (2019):62,
https://hdl.handle.net/21.15107/rcub_fiver_3331 .

TY  - CONF
AU  - Jovičić, Dušica
AU  - Petrović, Gordana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Marinković, Dragana
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2858
AB  - S obzirom na to da seme predstavlja početak biljne proizvodnje, obezbeđivanje kvaliteta semena bi trebalo da bude prioritet savremenog semenarstva i preduslov za visoke prinose svih biljnih vrsta. Pojam "kvalitet semena” predstavlja sveukupnu vrednost semena važnu za poljoprivrednu proizvodnju i čine ga različite karakteristike koje su od interesa za pojedine segmente industrije, pre svega za proizvođače, dorađivače i trgovce.
PB  - Novi Sad : Institut za ratarstvo i povrtarstvo
C3  - Zbornik referata, 52. Savetovanje agronoma i poljoprivrednika Srbije (SAPS) i 1. Savetovanje agronoma Republike Srbije i Republike Srpske, Zlatibor, 21-27.01.2018.
T1  - Tradicionalni i moderni pristup ispitivanju semena
EP  - 51
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2858
ER  - 

@conference{
author = "Jovičić, Dušica and Petrović, Gordana and Nikolić, Zorica and Tamindžić, Gordana and Ignjatov, Maja and Milošević, Dragana and Marinković, Dragana",
year = "2018",
abstract = "S obzirom na to da seme predstavlja početak biljne proizvodnje, obezbeđivanje kvaliteta semena bi trebalo da bude prioritet savremenog semenarstva i preduslov za visoke prinose svih biljnih vrsta. Pojam "kvalitet semena” predstavlja sveukupnu vrednost semena važnu za poljoprivrednu proizvodnju i čine ga različite karakteristike koje su od interesa za pojedine segmente industrije, pre svega za proizvođače, dorađivače i trgovce.",
publisher = "Novi Sad : Institut za ratarstvo i povrtarstvo",
journal = "Zbornik referata, 52. Savetovanje agronoma i poljoprivrednika Srbije (SAPS) i 1. Savetovanje agronoma Republike Srbije i Republike Srpske, Zlatibor, 21-27.01.2018.",
title = "Tradicionalni i moderni pristup ispitivanju semena",
pages = "51-45",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2858"
}

Jovičić, D., Petrović, G., Nikolić, Z., Tamindžić, G., Ignjatov, M., Milošević, D.,& Marinković, D.. (2018). Tradicionalni i moderni pristup ispitivanju semena. in Zbornik referata, 52. Savetovanje agronoma i poljoprivrednika Srbije (SAPS) i 1. Savetovanje agronoma Republike Srbije i Republike Srpske, Zlatibor, 21-27.01.2018.
Novi Sad : Institut za ratarstvo i povrtarstvo., 45-51.
https://hdl.handle.net/21.15107/rcub_fiver_2858

Jovičić D, Petrović G, Nikolić Z, Tamindžić G, Ignjatov M, Milošević D, Marinković D. Tradicionalni i moderni pristup ispitivanju semena. in Zbornik referata, 52. Savetovanje agronoma i poljoprivrednika Srbije (SAPS) i 1. Savetovanje agronoma Republike Srbije i Republike Srpske, Zlatibor, 21-27.01.2018.. 2018;:45-51.
https://hdl.handle.net/21.15107/rcub_fiver_2858 .

Jovičić, Dušica, Petrović, Gordana, Nikolić, Zorica, Tamindžić, Gordana, Ignjatov, Maja, Milošević, Dragana, Marinković, Dragana, "Tradicionalni i moderni pristup ispitivanju semena" in Zbornik referata, 52. Savetovanje agronoma i poljoprivrednika Srbije (SAPS) i 1. Savetovanje agronoma Republike Srbije i Republike Srpske, Zlatibor, 21-27.01.2018. (2018):45-51,
https://hdl.handle.net/21.15107/rcub_fiver_2858 .

TY  - CONF
AU  - Petrović, Gordana
AU  - Stikić, Radmila
AU  - Živanović, Tomislav
AU  - Nikolić, Zorica
AU  - Marjanović, Milena
AU  - Petrović, Ivana
AU  - Samardžić, Jelena
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/3089
AB  - Izlaganje stresnim uslovima životne sredine, kao što je suša, ima negativan efekat na rast biljaka. Abcisinska kiselina (ABA) se akumulira u uslovima osmotskog stresa i igra važnu ulogu u odgovoru na stres i u toleranciji biljaka. Cilj ovog istraživanja je bio da se odredi koncentracija ABA i ekspresija aldehid oksidaznog enzima (AO) kod 7 sorti stočnog graška u početnim fazama klijanja semena i u stadijumu trećeg para listova. Osmotski stres je stimulisan primenom PEG 6000. Stres suše je izazvan prekidanjem zalivanja. Koncentracija ABA je merena Elisa metodom. U nadzemnom delu ponika utvrđeno je značajno povećanje sadržaja ABA kod jedne grupe sorti (Javor, Partner, Junior) i smanjenje koncentracije ABA kod drugih sorti (Mraz, Dukat, Pionir, Trezor).
AB  - Exposure to to environmental stresses, such as drought, causes adverse effects on the growth of plants. Abscisic acid (ABA) accumulates under osmotic stress conditions and plays an important role in the stress response and tolerance of plants. The aim of this research was to determine the rates of ABA and the expression of Aldehyde oxidase enzyme (AO) among 7 cultivars of field pea at early seedling stage and at stage of third pair of leaves. Osmotic stress was stimulated using PEG 6000. The drought stress was induced by a termination of irrigation. ABA concentration was measured by the Elisa method. In the shoot of seedlings a significant increase in ABA content in one group of cultivars (Javor, Partner, Junior), and a decrease in ABA concentration in other cultivars (Mraz, Dukat, Pionir, Trezor) was confirmed.
PB  - Beograd : Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
C3  - Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018.
T1  - Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode
T1  - Accumulation rates of abscisic acid and aldehyde oxidase activity in Pisum sativum L. in response to water deficit
EP  - 38
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3089
ER  - 

@conference{
author = "Petrović, Gordana and Stikić, Radmila and Živanović, Tomislav and Nikolić, Zorica and Marjanović, Milena and Petrović, Ivana and Samardžić, Jelena",
year = "2018",
abstract = "Izlaganje stresnim uslovima životne sredine, kao što je suša, ima negativan efekat na rast biljaka. Abcisinska kiselina (ABA) se akumulira u uslovima osmotskog stresa i igra važnu ulogu u odgovoru na stres i u toleranciji biljaka. Cilj ovog istraživanja je bio da se odredi koncentracija ABA i ekspresija aldehid oksidaznog enzima (AO) kod 7 sorti stočnog graška u početnim fazama klijanja semena i u stadijumu trećeg para listova. Osmotski stres je stimulisan primenom PEG 6000. Stres suše je izazvan prekidanjem zalivanja. Koncentracija ABA je merena Elisa metodom. U nadzemnom delu ponika utvrđeno je značajno povećanje sadržaja ABA kod jedne grupe sorti (Javor, Partner, Junior) i smanjenje koncentracije ABA kod drugih sorti (Mraz, Dukat, Pionir, Trezor)., Exposure to to environmental stresses, such as drought, causes adverse effects on the growth of plants. Abscisic acid (ABA) accumulates under osmotic stress conditions and plays an important role in the stress response and tolerance of plants. The aim of this research was to determine the rates of ABA and the expression of Aldehyde oxidase enzyme (AO) among 7 cultivars of field pea at early seedling stage and at stage of third pair of leaves. Osmotic stress was stimulated using PEG 6000. The drought stress was induced by a termination of irrigation. ABA concentration was measured by the Elisa method. In the shoot of seedlings a significant increase in ABA content in one group of cultivars (Javor, Partner, Junior), and a decrease in ABA concentration in other cultivars (Mraz, Dukat, Pionir, Trezor) was confirmed.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018.",
title = "Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode, Accumulation rates of abscisic acid and aldehyde oxidase activity in Pisum sativum L. in response to water deficit",
pages = "38-37",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3089"
}

Petrović, G., Stikić, R., Živanović, T., Nikolić, Z., Marjanović, M., Petrović, I.,& Samardžić, J.. (2018). Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode. in Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018.
Beograd : Društvo genetičara Srbije., 37-38.
https://hdl.handle.net/21.15107/rcub_fiver_3089

Petrović G, Stikić R, Živanović T, Nikolić Z, Marjanović M, Petrović I, Samardžić J. Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode. in Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018.. 2018;:37-38.
https://hdl.handle.net/21.15107/rcub_fiver_3089 .

Petrović, Gordana, Stikić, Radmila, Živanović, Tomislav, Nikolić, Zorica, Marjanović, Milena, Petrović, Ivana, Samardžić, Jelena, "Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode" in Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018. (2018):37-38,
https://hdl.handle.net/21.15107/rcub_fiver_3089 .

TY  - JOUR
AU  - Župunski, Vesna
AU  - Vasić, Mirjana
AU  - Sustar-Vozlić, Jelka
AU  - Maras, Marko
AU  - Savić, Aleksandra
AU  - Petrović, Gordana
AU  - Živanov, Dalibor
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1804
AB  - Trypsin inhibitors could limit utilization of legumes in human nutrition, but they could also have beneficial health effects. The objective of this study was to measure trypsin inhibitor activity (TIA) of different legumes using microtiter plate method and to identify factors that contribute to uncertainty of TIA measurement. TIA measurements were performed on seeds of faba bean, pea, common vetch, soybean, and common bean cultivars. The significant effect of legume crop on TIA measurement uncertainty was confirmed with P = 0.045. Certain sources of measurement uncertainty were related with the content of trypsin inhibitors (Tis) in legume seeds. In respect to that, significant effect of level of sample dilution (P E, 0.001) was confirmed. Significant influence of the repeated absorbance measurement of sample reaction mixture on uncertainty of TIA measurement was identified (P E, 0.001), and it took 60% of overall TIA measurement uncertainty for soybean cultivars. TIA of soybean cultivars exceeded 90 TUI/mg. Repeated absorbance measurement of positive control reaction mixture took 70% of TIA measurement uncertainty of cultivars with TIA lesser than 4.5 TUI/mg. Graduated cylinder used for preparation of the final sample solutions took the range from 45 to 90% of overall TIA measurement uncertainty of the cultivars whose TIA were in the middle of previously mentioned. The uncertainty of TIA measurement of legume crops was not studied before; thus, this study pointed out that acquiring insight into factors contributing to uncertainty of TIA measurement could give directions for improvement of TIA testing if microtiter plate method is used.
PB  - Springer, New York
T2  - Food Analytical Methods
T1  - Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method
EP  - 1040
IS  - 4
SP  - 1034
VL  - 11
DO  - 10.1007/s12161-017-1076-y
ER  - 

@article{
author = "Župunski, Vesna and Vasić, Mirjana and Sustar-Vozlić, Jelka and Maras, Marko and Savić, Aleksandra and Petrović, Gordana and Živanov, Dalibor",
year = "2018",
abstract = "Trypsin inhibitors could limit utilization of legumes in human nutrition, but they could also have beneficial health effects. The objective of this study was to measure trypsin inhibitor activity (TIA) of different legumes using microtiter plate method and to identify factors that contribute to uncertainty of TIA measurement. TIA measurements were performed on seeds of faba bean, pea, common vetch, soybean, and common bean cultivars. The significant effect of legume crop on TIA measurement uncertainty was confirmed with P = 0.045. Certain sources of measurement uncertainty were related with the content of trypsin inhibitors (Tis) in legume seeds. In respect to that, significant effect of level of sample dilution (P E, 0.001) was confirmed. Significant influence of the repeated absorbance measurement of sample reaction mixture on uncertainty of TIA measurement was identified (P E, 0.001), and it took 60% of overall TIA measurement uncertainty for soybean cultivars. TIA of soybean cultivars exceeded 90 TUI/mg. Repeated absorbance measurement of positive control reaction mixture took 70% of TIA measurement uncertainty of cultivars with TIA lesser than 4.5 TUI/mg. Graduated cylinder used for preparation of the final sample solutions took the range from 45 to 90% of overall TIA measurement uncertainty of the cultivars whose TIA were in the middle of previously mentioned. The uncertainty of TIA measurement of legume crops was not studied before; thus, this study pointed out that acquiring insight into factors contributing to uncertainty of TIA measurement could give directions for improvement of TIA testing if microtiter plate method is used.",
publisher = "Springer, New York",
journal = "Food Analytical Methods",
title = "Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method",
pages = "1040-1034",
number = "4",
volume = "11",
doi = "10.1007/s12161-017-1076-y"
}

Župunski, V., Vasić, M., Sustar-Vozlić, J., Maras, M., Savić, A., Petrović, G.,& Živanov, D.. (2018). Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method. in Food Analytical Methods
Springer, New York., 11(4), 1034-1040.
https://doi.org/10.1007/s12161-017-1076-y

Župunski V, Vasić M, Sustar-Vozlić J, Maras M, Savić A, Petrović G, Živanov D. Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method. in Food Analytical Methods. 2018;11(4):1034-1040.
doi:10.1007/s12161-017-1076-y .

Župunski, Vesna, Vasić, Mirjana, Sustar-Vozlić, Jelka, Maras, Marko, Savić, Aleksandra, Petrović, Gordana, Živanov, Dalibor, "Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method" in Food Analytical Methods, 11, no. 4 (2018):1034-1040,
https://doi.org/10.1007/s12161-017-1076-y . .

TY  - JOUR
AU  - Živanov, Dalibor
AU  - Vasiljević, Sanja
AU  - Nikolić, Zorica
AU  - Đorđević, Vuk
AU  - Ramazanova, Rausha
AU  - Milošević, Branko
AU  - Petrović, Gordana
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1732
AB  - The aim of this work was to estimate the trypsin inhibitor activity (TIA) in seeds of cultivated pea varieties, wild varieties and selected crosses between varieties from the first group and wild pea varieties and to study the variation in genes coding trypsin inhibitors. Mean TIA in field pea varieties ranged from 3.12 TIU/mg of sample in field pea variety from Czech Republic to 12.90 TIU/mg of sample in field pea variety FP S4 of Serbian origin. Wild field pea varieties showed TIA between 0.98 TIU/mg of sample in Pisum elatius and 9.79 TIU/mg of sample in Pisum abyssinicum. Selected crosses between cultivated field pea varieties and Pisum elatius showed a decrease in TIA in comparison with a parent line that has higher TIA content. The PCR amplification resulted in variety-specific amplification. Varieties with low TIA activity showed amplification with At13/At5 primer pair, while varieties with higher TIA activity showed amplification with primer pairs At12/At5, At14/At5 and At14/At8. Thus, At13/At5 primer pair could be sufficient to distinguish most varieties. These matters can be applied during an early screening of the valuable materials for future breeding programs of pea cultivars with the low level of tripsin inhibitor.
PB  - Springer, Dordrecht
T2  - Molecular Breeding
T1  - A potential use of wild pea as a source of lower trypsin inhibitor activity
IS  - 8
VL  - 38
DO  - 10.1007/s11032-018-0862-6
ER  - 

@article{
author = "Živanov, Dalibor and Vasiljević, Sanja and Nikolić, Zorica and Đorđević, Vuk and Ramazanova, Rausha and Milošević, Branko and Petrović, Gordana",
year = "2018",
abstract = "The aim of this work was to estimate the trypsin inhibitor activity (TIA) in seeds of cultivated pea varieties, wild varieties and selected crosses between varieties from the first group and wild pea varieties and to study the variation in genes coding trypsin inhibitors. Mean TIA in field pea varieties ranged from 3.12 TIU/mg of sample in field pea variety from Czech Republic to 12.90 TIU/mg of sample in field pea variety FP S4 of Serbian origin. Wild field pea varieties showed TIA between 0.98 TIU/mg of sample in Pisum elatius and 9.79 TIU/mg of sample in Pisum abyssinicum. Selected crosses between cultivated field pea varieties and Pisum elatius showed a decrease in TIA in comparison with a parent line that has higher TIA content. The PCR amplification resulted in variety-specific amplification. Varieties with low TIA activity showed amplification with At13/At5 primer pair, while varieties with higher TIA activity showed amplification with primer pairs At12/At5, At14/At5 and At14/At8. Thus, At13/At5 primer pair could be sufficient to distinguish most varieties. These matters can be applied during an early screening of the valuable materials for future breeding programs of pea cultivars with the low level of tripsin inhibitor.",
publisher = "Springer, Dordrecht",
journal = "Molecular Breeding",
title = "A potential use of wild pea as a source of lower trypsin inhibitor activity",
number = "8",
volume = "38",
doi = "10.1007/s11032-018-0862-6"
}

Živanov, D., Vasiljević, S., Nikolić, Z., Đorđević, V., Ramazanova, R., Milošević, B.,& Petrović, G.. (2018). A potential use of wild pea as a source of lower trypsin inhibitor activity. in Molecular Breeding
Springer, Dordrecht., 38(8).
https://doi.org/10.1007/s11032-018-0862-6

Živanov D, Vasiljević S, Nikolić Z, Đorđević V, Ramazanova R, Milošević B, Petrović G. A potential use of wild pea as a source of lower trypsin inhibitor activity. in Molecular Breeding. 2018;38(8).
doi:10.1007/s11032-018-0862-6 .

Živanov, Dalibor, Vasiljević, Sanja, Nikolić, Zorica, Đorđević, Vuk, Ramazanova, Rausha, Milošević, Branko, Petrović, Gordana, "A potential use of wild pea as a source of lower trypsin inhibitor activity" in Molecular Breeding, 38, no. 8 (2018),
https://doi.org/10.1007/s11032-018-0862-6 . .

TY  - JOUR
AU  - Jovičić, Dušica
AU  - Vasin, Jovica
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Tamindžić, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/3246
AB  - This work considers the results obtained by testing the parameters of antioxidant status in leaves and roots of oilseed rape at different growth stages (phase I: formation of leaf rosettes and preparing for winter hibernation; phase II: after the winter hibernation; phase III: flowering) grown in semicontrolled field conditions on different soil types. The experiment was conducted on four oilseed rape genotypes (Banacanka, Jasna, Kata, and Zlatna) in semicontrolled conditions in containers with three soil types: chernozem, solonetz, and solonchak. The germination failed on solonchak due to the excessive amount of salt, but results were obtained for plant material on chernozem and solonetz. The following parameters were examined: superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GPx) activity, lipid peroxidation (LP) intensity, glutathione (GSH) content, and total antioxidant capacity (by ferric reducing antioxidant power, FRAP method). The activity of all the tested parameters of antioxidant status in all genotypes, both in leaves and roots, was higher in plants grown on solonetz soil compared to plants grown on chernozem. Furthermore, the activity of all the tested parameters on both soil types was highest in the second phase of testing, the stage after the winter hibernation, because the plants were exposed to low temperatures in addition to salt stress. The vital thing to note is that GPx activity is important for the defense at the initial stages of growth, while in the later growth stages SOD assumes this role. The genotype Banacanka has been distinguished as the most tolerant, while the genotype Zlatna was the most sensitive.
PB  - Ankara : Scientific and Technological Research Council of Turkey (TUBITAK) - Turkish Academic Network and Information Center (ULAKBIM)
T2  - Turkish Journal of Agriculture and Forestry
T1  - Antioxidant capacity of oilseed rape (Brassica napus) in different soil types
EP  - 471
SP  - 463
VL  - 41
DO  - 10.3906/tar-1701-94
ER  - 

@article{
author = "Jovičić, Dušica and Vasin, Jovica and Nikolić, Zorica and Petrović, Gordana and Tamindžić, Gordana and Ignjatov, Maja and Milošević, Dragana",
year = "2017",
abstract = "This work considers the results obtained by testing the parameters of antioxidant status in leaves and roots of oilseed rape at different growth stages (phase I: formation of leaf rosettes and preparing for winter hibernation; phase II: after the winter hibernation; phase III: flowering) grown in semicontrolled field conditions on different soil types. The experiment was conducted on four oilseed rape genotypes (Banacanka, Jasna, Kata, and Zlatna) in semicontrolled conditions in containers with three soil types: chernozem, solonetz, and solonchak. The germination failed on solonchak due to the excessive amount of salt, but results were obtained for plant material on chernozem and solonetz. The following parameters were examined: superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GPx) activity, lipid peroxidation (LP) intensity, glutathione (GSH) content, and total antioxidant capacity (by ferric reducing antioxidant power, FRAP method). The activity of all the tested parameters of antioxidant status in all genotypes, both in leaves and roots, was higher in plants grown on solonetz soil compared to plants grown on chernozem. Furthermore, the activity of all the tested parameters on both soil types was highest in the second phase of testing, the stage after the winter hibernation, because the plants were exposed to low temperatures in addition to salt stress. The vital thing to note is that GPx activity is important for the defense at the initial stages of growth, while in the later growth stages SOD assumes this role. The genotype Banacanka has been distinguished as the most tolerant, while the genotype Zlatna was the most sensitive.",
publisher = "Ankara : Scientific and Technological Research Council of Turkey (TUBITAK) - Turkish Academic Network and Information Center (ULAKBIM)",
journal = "Turkish Journal of Agriculture and Forestry",
title = "Antioxidant capacity of oilseed rape (Brassica napus) in different soil types",
pages = "471-463",
volume = "41",
doi = "10.3906/tar-1701-94"
}

Jovičić, D., Vasin, J., Nikolić, Z., Petrović, G., Tamindžić, G., Ignjatov, M.,& Milošević, D.. (2017). Antioxidant capacity of oilseed rape (Brassica napus) in different soil types. in Turkish Journal of Agriculture and Forestry
Ankara : Scientific and Technological Research Council of Turkey (TUBITAK) - Turkish Academic Network and Information Center (ULAKBIM)., 41, 463-471.
https://doi.org/10.3906/tar-1701-94

Jovičić D, Vasin J, Nikolić Z, Petrović G, Tamindžić G, Ignjatov M, Milošević D. Antioxidant capacity of oilseed rape (Brassica napus) in different soil types. in Turkish Journal of Agriculture and Forestry. 2017;41:463-471.
doi:10.3906/tar-1701-94 .

Jovičić, Dušica, Vasin, Jovica, Nikolić, Zorica, Petrović, Gordana, Tamindžić, Gordana, Ignjatov, Maja, Milošević, Dragana, "Antioxidant capacity of oilseed rape (Brassica napus) in different soil types" in Turkish Journal of Agriculture and Forestry, 41 (2017):463-471,
https://doi.org/10.3906/tar-1701-94 . .

TY  - JOUR
AU  - Jovičić, Dušica
AU  - Vasin, Jovica
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Tamindžić, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1698
AB  - This work considers the results obtained by testing the parameters of antioxidant status in leaves and roots of oilseed rape at different growth stages (phase I: formation of leaf rosettes and preparing for winter hibernation; phase II: after the winter hibernation; phase III: flowering) grown in semicontrolled field conditions on different soil types. The experiment was conducted on four oilseed rape genotypes (Banacanka, Jasna, Kata, and Zlatna) in semicontrolled conditions in containers with three soil types: chernozem, solonetz, and solonchak. The germination failed on solonchak due to the excessive amount of salt, but results were obtained for plant material on chernozem and solonetz. The following parameters were examined: superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GPx) activity, lipid peroxidation (LP) intensity, glutathione (GSH) content, and total antioxidant capacity (by ferric reducing antioxidant power, FRAP method). The activity of all the tested parameters of antioxidant status in all genotypes, both in leaves and roots, was higher in plants grown on solonetz soil compared to plants grown on chernozem. Furthermore, the activity of all the tested parameters on both soil types was highest in the second phase of testing, the stage after the winter hibernation, because the plants were exposed to low temperatures in addition to salt stress. The vital thing to note is that GPx activity is important for the defense at the initial stages of growth, while in the later growth stages SOD assumes this role. The genotype Banacanka has been distinguished as the most tolerant, while the genotype Zlatna was the most sensitive.
PB  - Tubitak Scientific & Technical Research Council Turkey, Ankara
T2  - Turkish Journal of Agriculture & Forestry
T1  - Antioxidant capacity of oilseed rape (&ITBrassica napus&IT) in different soil types
EP  - 471
IS  - 6
SP  - 463
VL  - 41
DO  - 10.3906/tar-1701-94
ER  - 

@article{
author = "Jovičić, Dušica and Vasin, Jovica and Nikolić, Zorica and Petrović, Gordana and Tamindžić, Gordana and Ignjatov, Maja and Milošević, Dragana",
year = "2017",
abstract = "This work considers the results obtained by testing the parameters of antioxidant status in leaves and roots of oilseed rape at different growth stages (phase I: formation of leaf rosettes and preparing for winter hibernation; phase II: after the winter hibernation; phase III: flowering) grown in semicontrolled field conditions on different soil types. The experiment was conducted on four oilseed rape genotypes (Banacanka, Jasna, Kata, and Zlatna) in semicontrolled conditions in containers with three soil types: chernozem, solonetz, and solonchak. The germination failed on solonchak due to the excessive amount of salt, but results were obtained for plant material on chernozem and solonetz. The following parameters were examined: superoxide dismutase (SOD) enzyme activity, glutathione peroxidase (GPx) activity, lipid peroxidation (LP) intensity, glutathione (GSH) content, and total antioxidant capacity (by ferric reducing antioxidant power, FRAP method). The activity of all the tested parameters of antioxidant status in all genotypes, both in leaves and roots, was higher in plants grown on solonetz soil compared to plants grown on chernozem. Furthermore, the activity of all the tested parameters on both soil types was highest in the second phase of testing, the stage after the winter hibernation, because the plants were exposed to low temperatures in addition to salt stress. The vital thing to note is that GPx activity is important for the defense at the initial stages of growth, while in the later growth stages SOD assumes this role. The genotype Banacanka has been distinguished as the most tolerant, while the genotype Zlatna was the most sensitive.",
publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara",
journal = "Turkish Journal of Agriculture & Forestry",
title = "Antioxidant capacity of oilseed rape (&ITBrassica napus&IT) in different soil types",
pages = "471-463",
number = "6",
volume = "41",
doi = "10.3906/tar-1701-94"
}

Jovičić, D., Vasin, J., Nikolić, Z., Petrović, G., Tamindžić, G., Ignjatov, M.,& Milošević, D.. (2017). Antioxidant capacity of oilseed rape (&ITBrassica napus&IT) in different soil types. in Turkish Journal of Agriculture & Forestry
Tubitak Scientific & Technical Research Council Turkey, Ankara., 41(6), 463-471.
https://doi.org/10.3906/tar-1701-94

Jovičić D, Vasin J, Nikolić Z, Petrović G, Tamindžić G, Ignjatov M, Milošević D. Antioxidant capacity of oilseed rape (&ITBrassica napus&IT) in different soil types. in Turkish Journal of Agriculture & Forestry. 2017;41(6):463-471.
doi:10.3906/tar-1701-94 .

Jovičić, Dušica, Vasin, Jovica, Nikolić, Zorica, Petrović, Gordana, Tamindžić, Gordana, Ignjatov, Maja, Milošević, Dragana, "Antioxidant capacity of oilseed rape (&ITBrassica napus&IT) in different soil types" in Turkish Journal of Agriculture & Forestry, 41, no. 6 (2017):463-471,
https://doi.org/10.3906/tar-1701-94 . .

TY  - JOUR
AU  - Jovičić, Dušica
AU  - Štajner, Dubravka
AU  - Popović, Boris M.
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Zdero-Pavlović, Ruzica
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1683
AB  - This work was aimed to investigate the effect of salt (NaCl) stress on three oilseed rape (Brassica napus L.) cultivars: 'Banacanka', 'Jasna' and 'Kata'. The following parameters were determined: antioxidant enzymes superoxide dismutase (SOD) and guaiacol peroxidase (GPX), reduced glutathione (GSH) quantity, lipid peroxidation (LP) intensity, radical-scavenging capacity (DPPH-RSC), hydroxyl radical scavenging ((OH)-O-center dot radicals) and ferric reducing antioxidant power (FRAP). Seed viability was determined using the standard laboratory test for germination as well as the seed accelerated aging test measuring the germination, shoot and root length. The plants were subjected to three salt treatments: 0, 100, 150 and 200 mmol dm(-3) NaCl for 7 days. All three cultivars responded with an increase of lipid peroxidation in seedlings and with a decrease of GPX activity. 'Banacanka' was found to be the most vulnerable cultivar, with an increase of 237% of LP intensity in comparison to the control at the highest NaCl concentration. The induction of the SOD activity was observed for all three cultivars by different NaCl concentrations. The antioxidant capacity measured by FRAP, DPPH-RSC and (OH)-O-center dot radicals was also induced, especially FRAP at the highest concentration in the cultivar 'Jasna' (by 282%). Germination, shoot and root length decreased for all investigated cultivars. Our results showed that cultivar 'Jasna' is the most adapted to salt stress because of the highest SOD activity and accumulation of antioxidants. Furthermore, this research suggests that some of the antioxidant parameters can be useful biochemical markers for the selection of oilseed rape tolerant cultivars under the conditions of salinity.
PB  - Lithuanian Research Centre Agriculture & Forestry, Kedainiu R
T2  - Zemdirbyste-Agriculture
T1  - Salt-induced changes in the antioxidant system and viability of oilseed rape
EP  - 258
IS  - 3
SP  - 249
VL  - 104
DO  - 10.13080/z-a.2017.104.032
ER  - 

@article{
author = "Jovičić, Dušica and Štajner, Dubravka and Popović, Boris M. and Marjanović-Jeromela, Ana and Nikolić, Zorica and Petrović, Gordana and Zdero-Pavlović, Ruzica",
year = "2017",
abstract = "This work was aimed to investigate the effect of salt (NaCl) stress on three oilseed rape (Brassica napus L.) cultivars: 'Banacanka', 'Jasna' and 'Kata'. The following parameters were determined: antioxidant enzymes superoxide dismutase (SOD) and guaiacol peroxidase (GPX), reduced glutathione (GSH) quantity, lipid peroxidation (LP) intensity, radical-scavenging capacity (DPPH-RSC), hydroxyl radical scavenging ((OH)-O-center dot radicals) and ferric reducing antioxidant power (FRAP). Seed viability was determined using the standard laboratory test for germination as well as the seed accelerated aging test measuring the germination, shoot and root length. The plants were subjected to three salt treatments: 0, 100, 150 and 200 mmol dm(-3) NaCl for 7 days. All three cultivars responded with an increase of lipid peroxidation in seedlings and with a decrease of GPX activity. 'Banacanka' was found to be the most vulnerable cultivar, with an increase of 237% of LP intensity in comparison to the control at the highest NaCl concentration. The induction of the SOD activity was observed for all three cultivars by different NaCl concentrations. The antioxidant capacity measured by FRAP, DPPH-RSC and (OH)-O-center dot radicals was also induced, especially FRAP at the highest concentration in the cultivar 'Jasna' (by 282%). Germination, shoot and root length decreased for all investigated cultivars. Our results showed that cultivar 'Jasna' is the most adapted to salt stress because of the highest SOD activity and accumulation of antioxidants. Furthermore, this research suggests that some of the antioxidant parameters can be useful biochemical markers for the selection of oilseed rape tolerant cultivars under the conditions of salinity.",
publisher = "Lithuanian Research Centre Agriculture & Forestry, Kedainiu R",
journal = "Zemdirbyste-Agriculture",
title = "Salt-induced changes in the antioxidant system and viability of oilseed rape",
pages = "258-249",
number = "3",
volume = "104",
doi = "10.13080/z-a.2017.104.032"
}

Jovičić, D., Štajner, D., Popović, B. M., Marjanović-Jeromela, A., Nikolić, Z., Petrović, G.,& Zdero-Pavlović, R.. (2017). Salt-induced changes in the antioxidant system and viability of oilseed rape. in Zemdirbyste-Agriculture
Lithuanian Research Centre Agriculture & Forestry, Kedainiu R., 104(3), 249-258.
https://doi.org/10.13080/z-a.2017.104.032

Jovičić D, Štajner D, Popović BM, Marjanović-Jeromela A, Nikolić Z, Petrović G, Zdero-Pavlović R. Salt-induced changes in the antioxidant system and viability of oilseed rape. in Zemdirbyste-Agriculture. 2017;104(3):249-258.
doi:10.13080/z-a.2017.104.032 .

Jovičić, Dušica, Štajner, Dubravka, Popović, Boris M., Marjanović-Jeromela, Ana, Nikolić, Zorica, Petrović, Gordana, Zdero-Pavlović, Ruzica, "Salt-induced changes in the antioxidant system and viability of oilseed rape" in Zemdirbyste-Agriculture, 104, no. 3 (2017):249-258,
https://doi.org/10.13080/z-a.2017.104.032 . .

TY  - JOUR
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Panković, Dejana
AU  - Ignjatov, Maja
AU  - Marinković, Dragana
AU  - Stojanović, Milan
AU  - Đorđević, Vuk
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1659
AB  - The aim of this study is to assess DNA degradation, DNA amplification, and GMO quantity during tofu production. Soybean seeds were spiked with Roundup Ready (R) soybeans (RRS) at 0.9, 2, 3 and 5 % (by mass), to assess the level of RSS that would be of practical interest for threshold labelling. Real-time polymerase chain reaction (PCR) was more effective than conventional PCR in the analysis of raw soymilk, okara, boiled soymilk and tofu. The negative effect of grinding and mechanical manipulation was obvious in the okara sample prepared with 3 and 5 % RRS, where GMO content was reduced to (2.28 +/- 0.23) and (2.74 +/- 0.26) %, respectively. However, heating at 100 degrees C for 10 min did not cause significant degradation of DNA in all samples. The content of RRS in the final product, tofu, was reduced tenfold during processing, ranging from 0.07 to 0.46 %, which was below the labelling threshold level. The results are discussed in terms of global harmonization of GMO standards, which could have the positive effect on the trade of lightly processed foodstuffs such as tofu, especially regarding the labelling policies.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology & Biotechnology
T1  - Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production
EP  - 444
IS  - 4
SP  - 439
VL  - 55
DO  - 10.17113/ftb.55.04.17.5192
ER  - 

@article{
author = "Nikolić, Zorica and Petrović, Gordana and Panković, Dejana and Ignjatov, Maja and Marinković, Dragana and Stojanović, Milan and Đorđević, Vuk",
year = "2017",
abstract = "The aim of this study is to assess DNA degradation, DNA amplification, and GMO quantity during tofu production. Soybean seeds were spiked with Roundup Ready (R) soybeans (RRS) at 0.9, 2, 3 and 5 % (by mass), to assess the level of RSS that would be of practical interest for threshold labelling. Real-time polymerase chain reaction (PCR) was more effective than conventional PCR in the analysis of raw soymilk, okara, boiled soymilk and tofu. The negative effect of grinding and mechanical manipulation was obvious in the okara sample prepared with 3 and 5 % RRS, where GMO content was reduced to (2.28 +/- 0.23) and (2.74 +/- 0.26) %, respectively. However, heating at 100 degrees C for 10 min did not cause significant degradation of DNA in all samples. The content of RRS in the final product, tofu, was reduced tenfold during processing, ranging from 0.07 to 0.46 %, which was below the labelling threshold level. The results are discussed in terms of global harmonization of GMO standards, which could have the positive effect on the trade of lightly processed foodstuffs such as tofu, especially regarding the labelling policies.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology & Biotechnology",
title = "Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production",
pages = "444-439",
number = "4",
volume = "55",
doi = "10.17113/ftb.55.04.17.5192"
}

Nikolić, Z., Petrović, G., Panković, D., Ignjatov, M., Marinković, D., Stojanović, M.,& Đorđević, V.. (2017). Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production. in Food Technology & Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 55(4), 439-444.
https://doi.org/10.17113/ftb.55.04.17.5192

Nikolić Z, Petrović G, Panković D, Ignjatov M, Marinković D, Stojanović M, Đorđević V. Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production. in Food Technology & Biotechnology. 2017;55(4):439-444.
doi:10.17113/ftb.55.04.17.5192 .

Nikolić, Zorica, Petrović, Gordana, Panković, Dejana, Ignjatov, Maja, Marinković, Dragana, Stojanović, Milan, Đorđević, Vuk, "Threshold Level and Traceability of Roundup Ready (R) Soybeans in Tofu Production" in Food Technology & Biotechnology, 55, no. 4 (2017):439-444,
https://doi.org/10.17113/ftb.55.04.17.5192 . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović, Tatjana
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1628
AB  - Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).
AB  - Crni luk (Allium cepa L.) spada u najznačajnije povrtarske biljne vrste u Srbiji. Gaji se na površini od oko 20.000 hektara. Tokom rutinske kontrole kvaliteta semena crnog luka u 2014. godini, primećena je pojava Fusarium sp. u visokom procentu - od 28%. Cilj rada bio je izolacija i identifikacija Fusarium sp. na osnovu morfoloških i molekularnih karakteristika patogena. Uzorci semena luka sakupljeni su s različitih lokaliteta i iz velikog broja skladišta u Vojvodini. Nakon izolacije patogena odabrano je i morfološki okarakterisano 25 izolata Fusarium sp. Izolacija DNK izvršena je direktno iz micelija gljive (100 mg), korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Amplifikacija DNK ciljanog gena (translation elongation factor EF-1α gene) obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1α gena, koji je deponovan u NCBI bazu podataka pod brojem KP658211 (Fusarium sp. FIESC 3).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)
T1  - Identifikacija i filogenetska analiza Fusarium sp. FIESC 3 prouzrokovača truleži semena crnog luka (Allium cepa L.)
EP  - 17
IS  - 132
SP  - 9
DO  - 10.2298/ZMSPN1732009I
ER  - 

@article{
author = "Ignjatov, Maja and Popović, Tatjana and Milošević, Dragana and Nikolić, Zorica and Petrović, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2017",
abstract = "Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3)., Crni luk (Allium cepa L.) spada u najznačajnije povrtarske biljne vrste u Srbiji. Gaji se na površini od oko 20.000 hektara. Tokom rutinske kontrole kvaliteta semena crnog luka u 2014. godini, primećena je pojava Fusarium sp. u visokom procentu - od 28%. Cilj rada bio je izolacija i identifikacija Fusarium sp. na osnovu morfoloških i molekularnih karakteristika patogena. Uzorci semena luka sakupljeni su s različitih lokaliteta i iz velikog broja skladišta u Vojvodini. Nakon izolacije patogena odabrano je i morfološki okarakterisano 25 izolata Fusarium sp. Izolacija DNK izvršena je direktno iz micelija gljive (100 mg), korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Amplifikacija DNK ciljanog gena (translation elongation factor EF-1α gene) obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1α gena, koji je deponovan u NCBI bazu podataka pod brojem KP658211 (Fusarium sp. FIESC 3).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.), Identifikacija i filogenetska analiza Fusarium sp. FIESC 3 prouzrokovača truleži semena crnog luka (Allium cepa L.)",
pages = "17-9",
number = "132",
doi = "10.2298/ZMSPN1732009I"
}

Ignjatov, M., Popović, T., Milošević, D., Nikolić, Z., Petrović, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2017). Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(132), 9-17.
https://doi.org/10.2298/ZMSPN1732009I

Ignjatov M, Popović T, Milošević D, Nikolić Z, Petrović G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke. 2017;(132):9-17.
doi:10.2298/ZMSPN1732009I .

Ignjatov, Maja, Popović, Tatjana, Milošević, Dragana, Nikolić, Zorica, Petrović, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)" in Zbornik Matice srpske za prirodne nauke, no. 132 (2017):9-17,
https://doi.org/10.2298/ZMSPN1732009I . .