A potential use of wild pea as a source of lower trypsin inhibitor activity
Само за регистроване кориснике
2018
Аутори
Živanov, DaliborVasiljević, Sanja
Nikolić, Zorica
Đorđević, Vuk
Ramazanova, Rausha
Milošević, Branko
Petrović, Gordana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this work was to estimate the trypsin inhibitor activity (TIA) in seeds of cultivated pea varieties, wild varieties and selected crosses between varieties from the first group and wild pea varieties and to study the variation in genes coding trypsin inhibitors. Mean TIA in field pea varieties ranged from 3.12 TIU/mg of sample in field pea variety from Czech Republic to 12.90 TIU/mg of sample in field pea variety FP S4 of Serbian origin. Wild field pea varieties showed TIA between 0.98 TIU/mg of sample in Pisum elatius and 9.79 TIU/mg of sample in Pisum abyssinicum. Selected crosses between cultivated field pea varieties and Pisum elatius showed a decrease in TIA in comparison with a parent line that has higher TIA content. The PCR amplification resulted in variety-specific amplification. Varieties with low TIA activity showed amplification with At13/At5 primer pair, while varieties with higher TIA activity showed amplification with primer pairs At12/At5, At14/At5 and At14/At...8. Thus, At13/At5 primer pair could be sufficient to distinguish most varieties. These matters can be applied during an early screening of the valuable materials for future breeding programs of pea cultivars with the low level of tripsin inhibitor.
Кључне речи:
At5 / At8 / At12 / At13 / At14 / TIAИзвор:
Molecular Breeding, 2018, 38, 8Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Повећање тржишног значаја крмних биљака оплемењивањем и оптимизацијом технологије производње семена (RS-MESTD-Technological Development (TD or TR)-31024)
DOI: 10.1007/s11032-018-0862-6
ISSN: 1380-3743
WoS: 000441253300001
Scopus: 2-s2.0-85051530934
Колекције
Институција/група
FiVeRTY - JOUR AU - Živanov, Dalibor AU - Vasiljević, Sanja AU - Nikolić, Zorica AU - Đorđević, Vuk AU - Ramazanova, Rausha AU - Milošević, Branko AU - Petrović, Gordana PY - 2018 UR - http://fiver.ifvcns.rs/handle/123456789/1732 AB - The aim of this work was to estimate the trypsin inhibitor activity (TIA) in seeds of cultivated pea varieties, wild varieties and selected crosses between varieties from the first group and wild pea varieties and to study the variation in genes coding trypsin inhibitors. Mean TIA in field pea varieties ranged from 3.12 TIU/mg of sample in field pea variety from Czech Republic to 12.90 TIU/mg of sample in field pea variety FP S4 of Serbian origin. Wild field pea varieties showed TIA between 0.98 TIU/mg of sample in Pisum elatius and 9.79 TIU/mg of sample in Pisum abyssinicum. Selected crosses between cultivated field pea varieties and Pisum elatius showed a decrease in TIA in comparison with a parent line that has higher TIA content. The PCR amplification resulted in variety-specific amplification. Varieties with low TIA activity showed amplification with At13/At5 primer pair, while varieties with higher TIA activity showed amplification with primer pairs At12/At5, At14/At5 and At14/At8. Thus, At13/At5 primer pair could be sufficient to distinguish most varieties. These matters can be applied during an early screening of the valuable materials for future breeding programs of pea cultivars with the low level of tripsin inhibitor. PB - Springer, Dordrecht T2 - Molecular Breeding T1 - A potential use of wild pea as a source of lower trypsin inhibitor activity IS - 8 VL - 38 DO - 10.1007/s11032-018-0862-6 ER -
@article{ author = "Živanov, Dalibor and Vasiljević, Sanja and Nikolić, Zorica and Đorđević, Vuk and Ramazanova, Rausha and Milošević, Branko and Petrović, Gordana", year = "2018", abstract = "The aim of this work was to estimate the trypsin inhibitor activity (TIA) in seeds of cultivated pea varieties, wild varieties and selected crosses between varieties from the first group and wild pea varieties and to study the variation in genes coding trypsin inhibitors. Mean TIA in field pea varieties ranged from 3.12 TIU/mg of sample in field pea variety from Czech Republic to 12.90 TIU/mg of sample in field pea variety FP S4 of Serbian origin. Wild field pea varieties showed TIA between 0.98 TIU/mg of sample in Pisum elatius and 9.79 TIU/mg of sample in Pisum abyssinicum. Selected crosses between cultivated field pea varieties and Pisum elatius showed a decrease in TIA in comparison with a parent line that has higher TIA content. The PCR amplification resulted in variety-specific amplification. Varieties with low TIA activity showed amplification with At13/At5 primer pair, while varieties with higher TIA activity showed amplification with primer pairs At12/At5, At14/At5 and At14/At8. Thus, At13/At5 primer pair could be sufficient to distinguish most varieties. These matters can be applied during an early screening of the valuable materials for future breeding programs of pea cultivars with the low level of tripsin inhibitor.", publisher = "Springer, Dordrecht", journal = "Molecular Breeding", title = "A potential use of wild pea as a source of lower trypsin inhibitor activity", number = "8", volume = "38", doi = "10.1007/s11032-018-0862-6" }
Živanov, D., Vasiljević, S., Nikolić, Z., Đorđević, V., Ramazanova, R., Milošević, B.,& Petrović, G.. (2018). A potential use of wild pea as a source of lower trypsin inhibitor activity. in Molecular Breeding Springer, Dordrecht., 38(8). https://doi.org/10.1007/s11032-018-0862-6
Živanov D, Vasiljević S, Nikolić Z, Đorđević V, Ramazanova R, Milošević B, Petrović G. A potential use of wild pea as a source of lower trypsin inhibitor activity. in Molecular Breeding. 2018;38(8). doi:10.1007/s11032-018-0862-6 .
Živanov, Dalibor, Vasiljević, Sanja, Nikolić, Zorica, Đorđević, Vuk, Ramazanova, Rausha, Milošević, Branko, Petrović, Gordana, "A potential use of wild pea as a source of lower trypsin inhibitor activity" in Molecular Breeding, 38, no. 8 (2018), https://doi.org/10.1007/s11032-018-0862-6 . .