Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method
Само за регистроване кориснике
2018
Аутори
Župunski, VesnaVasić, Mirjana
Sustar-Vozlić, Jelka
Maras, Marko
Savić, Aleksandra
Petrović, Gordana
Živanov, Dalibor
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Trypsin inhibitors could limit utilization of legumes in human nutrition, but they could also have beneficial health effects. The objective of this study was to measure trypsin inhibitor activity (TIA) of different legumes using microtiter plate method and to identify factors that contribute to uncertainty of TIA measurement. TIA measurements were performed on seeds of faba bean, pea, common vetch, soybean, and common bean cultivars. The significant effect of legume crop on TIA measurement uncertainty was confirmed with P = 0.045. Certain sources of measurement uncertainty were related with the content of trypsin inhibitors (Tis) in legume seeds. In respect to that, significant effect of level of sample dilution (P E, 0.001) was confirmed. Significant influence of the repeated absorbance measurement of sample reaction mixture on uncertainty of TIA measurement was identified (P E, 0.001), and it took 60% of overall TIA measurement uncertainty for soybean cultivars. TIA of soybean cultiv...ars exceeded 90 TUI/mg. Repeated absorbance measurement of positive control reaction mixture took 70% of TIA measurement uncertainty of cultivars with TIA lesser than 4.5 TUI/mg. Graduated cylinder used for preparation of the final sample solutions took the range from 45 to 90% of overall TIA measurement uncertainty of the cultivars whose TIA were in the middle of previously mentioned. The uncertainty of TIA measurement of legume crops was not studied before; thus, this study pointed out that acquiring insight into factors contributing to uncertainty of TIA measurement could give directions for improvement of TIA testing if microtiter plate method is used.
Кључне речи:
Trypsin inhibitor activity / Faba bean / Pea / Common vetch / Soybean / Common beanИзвор:
Food Analytical Methods, 2018, 11, 4, 1034-1040Издавач:
- Springer, New York
Финансирање / пројекти:
- Повећање тржишног значаја крмних биљака оплемењивањем и оптимизацијом технологије производње семена (RS-MESTD-Technological Development (TD or TR)-31024)
- Стварање сората и хибрида поврћа за гајење на отвореном пољу и у заштићеном простору (RS-MESTD-Technological Development (TD or TR)-31030)
DOI: 10.1007/s12161-017-1076-y
ISSN: 1936-9751
WoS: 000426367000011
Scopus: 2-s2.0-85032690537
Колекције
Институција/група
FiVeRTY - JOUR AU - Župunski, Vesna AU - Vasić, Mirjana AU - Sustar-Vozlić, Jelka AU - Maras, Marko AU - Savić, Aleksandra AU - Petrović, Gordana AU - Živanov, Dalibor PY - 2018 UR - http://fiver.ifvcns.rs/handle/123456789/1804 AB - Trypsin inhibitors could limit utilization of legumes in human nutrition, but they could also have beneficial health effects. The objective of this study was to measure trypsin inhibitor activity (TIA) of different legumes using microtiter plate method and to identify factors that contribute to uncertainty of TIA measurement. TIA measurements were performed on seeds of faba bean, pea, common vetch, soybean, and common bean cultivars. The significant effect of legume crop on TIA measurement uncertainty was confirmed with P = 0.045. Certain sources of measurement uncertainty were related with the content of trypsin inhibitors (Tis) in legume seeds. In respect to that, significant effect of level of sample dilution (P E, 0.001) was confirmed. Significant influence of the repeated absorbance measurement of sample reaction mixture on uncertainty of TIA measurement was identified (P E, 0.001), and it took 60% of overall TIA measurement uncertainty for soybean cultivars. TIA of soybean cultivars exceeded 90 TUI/mg. Repeated absorbance measurement of positive control reaction mixture took 70% of TIA measurement uncertainty of cultivars with TIA lesser than 4.5 TUI/mg. Graduated cylinder used for preparation of the final sample solutions took the range from 45 to 90% of overall TIA measurement uncertainty of the cultivars whose TIA were in the middle of previously mentioned. The uncertainty of TIA measurement of legume crops was not studied before; thus, this study pointed out that acquiring insight into factors contributing to uncertainty of TIA measurement could give directions for improvement of TIA testing if microtiter plate method is used. PB - Springer, New York T2 - Food Analytical Methods T1 - Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method EP - 1040 IS - 4 SP - 1034 VL - 11 DO - 10.1007/s12161-017-1076-y ER -
@article{ author = "Župunski, Vesna and Vasić, Mirjana and Sustar-Vozlić, Jelka and Maras, Marko and Savić, Aleksandra and Petrović, Gordana and Živanov, Dalibor", year = "2018", abstract = "Trypsin inhibitors could limit utilization of legumes in human nutrition, but they could also have beneficial health effects. The objective of this study was to measure trypsin inhibitor activity (TIA) of different legumes using microtiter plate method and to identify factors that contribute to uncertainty of TIA measurement. TIA measurements were performed on seeds of faba bean, pea, common vetch, soybean, and common bean cultivars. The significant effect of legume crop on TIA measurement uncertainty was confirmed with P = 0.045. Certain sources of measurement uncertainty were related with the content of trypsin inhibitors (Tis) in legume seeds. In respect to that, significant effect of level of sample dilution (P E, 0.001) was confirmed. Significant influence of the repeated absorbance measurement of sample reaction mixture on uncertainty of TIA measurement was identified (P E, 0.001), and it took 60% of overall TIA measurement uncertainty for soybean cultivars. TIA of soybean cultivars exceeded 90 TUI/mg. Repeated absorbance measurement of positive control reaction mixture took 70% of TIA measurement uncertainty of cultivars with TIA lesser than 4.5 TUI/mg. Graduated cylinder used for preparation of the final sample solutions took the range from 45 to 90% of overall TIA measurement uncertainty of the cultivars whose TIA were in the middle of previously mentioned. The uncertainty of TIA measurement of legume crops was not studied before; thus, this study pointed out that acquiring insight into factors contributing to uncertainty of TIA measurement could give directions for improvement of TIA testing if microtiter plate method is used.", publisher = "Springer, New York", journal = "Food Analytical Methods", title = "Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method", pages = "1040-1034", number = "4", volume = "11", doi = "10.1007/s12161-017-1076-y" }
Župunski, V., Vasić, M., Sustar-Vozlić, J., Maras, M., Savić, A., Petrović, G.,& Živanov, D.. (2018). Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method. in Food Analytical Methods Springer, New York., 11(4), 1034-1040. https://doi.org/10.1007/s12161-017-1076-y
Župunski V, Vasić M, Sustar-Vozlić J, Maras M, Savić A, Petrović G, Živanov D. Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method. in Food Analytical Methods. 2018;11(4):1034-1040. doi:10.1007/s12161-017-1076-y .
Župunski, Vesna, Vasić, Mirjana, Sustar-Vozlić, Jelka, Maras, Marko, Savić, Aleksandra, Petrović, Gordana, Živanov, Dalibor, "Uncertainty of Trypsin Inhibitor Activity Measurement of Legume Crops Using Microtiter Plate Method" in Food Analytical Methods, 11, no. 4 (2018):1034-1040, https://doi.org/10.1007/s12161-017-1076-y . .