Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia
Аутори
Milošević, DraganaIgnjatov, Maja
Nikolić, Zorica
Tamindžić, Gordana
Petrović, Gordana
Vlajić, Slobodan
Stanković, Ivana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In the 2018–19 growing season, a total of 51 leaves of eggplant plants grown under field conditions were collected randomly from nine private gardens at four different localities in the Province of Vojvodina. Eggplants with nearly 40% of plants showing bright yellow to white mosaic or mottling of leaves were found throughout the inspected fields (gardens). The collected samples were analyzed for the presence of alfalfa mosaic virus (AMV), cucumber mosaic virus (CMV) and potato virus Y (PVY) using commercial double-antibody sandwich (DAS)-ELISA kits. Serological analysis of eggplant samples revealed the presence of AMV in 80.39% collected samples. None of the analyzed samples was positive for CMV and PVY. The virus was successfully mechanically transmitted to test plants including Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, as well as eggplant seedlings, confirming the infectious nature of the disease. The presence of AMV in eggplants was further verified by reverse tra...nscription–polymerase chain reaction (RT-PCR) and sequencing, using the primers CP AMV1 and CP AMV2 that amplify part of the coat protein (CP) gene. The phylogenetic analysis showed that Serbian AMV isolates grouped into a separate well-supported group together with AMV isolates from Italy, Croatia and previously characterized isolates from Serbia. To our knowledge, this is the first report of AMV infection of eggplant in Serbia.
Tokom 2018. i 2019. godine, prikupljen je 51 uzorak biljaka plavog patlidžana iz devet useva sa četiri različita lokaliteta gajenja ove biljne vrste u Vojvodini. Tokom pregleda, na blizu 40% biljaka plavog patlidžana mogli su se uočiti simptomi u vidu žućkastobeličastih pega raspoređenih po čitavoj površini liske. Prikupljeni uzorci analizirani su na prisustvo virusa mozaika lucerke (alfalfa mosaic virus, AMV), virusa mozaika krastavca (cucumber mosaic virus, CMV) i Y virusa krompira (potato virus Y, PVY) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Serološkim analizama utvrđeno je prisustvo AMV u 80,39% prikupljenih uzoraka. Prisustvo CMV i PVY nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, kao i na klijance plavog patlidžana, čime je potvrđena infe ktivna priroda oboljenja. Prisustvo AMV u biljkama plavog patlidžana je dalje potvrđeno primenom R...T-PCR metode (reverzna transkripcija praćena lančanom reakcijom polimeraze) i sekvencioniranjem odabranih izolata korišćenjem specifičnih prajmera CP AMV1 i CP AMV2, koji omogućavaju umnožavanje gena za protein omotača. Filogenetska analiza pokazala je grupisanje srpskih AMV izolata u jasno odvojenu grupu zajedno sa AMV izolatima iz Italije, Hrvatske i prethodno okarakterisanim izolatima iz Srbije. Prema našim saznanjima, ovo je prvi izveštaj o prisustvu virusa mozaika lucerke na plavom patlidžanu u Srbiji.
Кључне речи:
alfalfa mosaic virus / DAS-ELISA / RT-PCR / phylogenetic analysis / eggplant / virus mozaika lucerke / filogenetska analiza / plavi patlidžanИзвор:
Acta Agriculturae Serbica, 2021, 26, 51, 33-39Издавач:
- Čačak : Univerzitet u Kragujevcu, Agronomski fakultet u Čačku
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200032 (Научни институт за ратарство и повртарство, Нови Сад) (RS-MESTD-inst-2020-200032)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
Колекције
Институција/група
FiVeRTY - JOUR AU - Milošević, Dragana AU - Ignjatov, Maja AU - Nikolić, Zorica AU - Tamindžić, Gordana AU - Petrović, Gordana AU - Vlajić, Slobodan AU - Stanković, Ivana PY - 2021 UR - http://fiver.ifvcns.rs/handle/123456789/3327 AB - In the 2018–19 growing season, a total of 51 leaves of eggplant plants grown under field conditions were collected randomly from nine private gardens at four different localities in the Province of Vojvodina. Eggplants with nearly 40% of plants showing bright yellow to white mosaic or mottling of leaves were found throughout the inspected fields (gardens). The collected samples were analyzed for the presence of alfalfa mosaic virus (AMV), cucumber mosaic virus (CMV) and potato virus Y (PVY) using commercial double-antibody sandwich (DAS)-ELISA kits. Serological analysis of eggplant samples revealed the presence of AMV in 80.39% collected samples. None of the analyzed samples was positive for CMV and PVY. The virus was successfully mechanically transmitted to test plants including Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, as well as eggplant seedlings, confirming the infectious nature of the disease. The presence of AMV in eggplants was further verified by reverse transcription–polymerase chain reaction (RT-PCR) and sequencing, using the primers CP AMV1 and CP AMV2 that amplify part of the coat protein (CP) gene. The phylogenetic analysis showed that Serbian AMV isolates grouped into a separate well-supported group together with AMV isolates from Italy, Croatia and previously characterized isolates from Serbia. To our knowledge, this is the first report of AMV infection of eggplant in Serbia. AB - Tokom 2018. i 2019. godine, prikupljen je 51 uzorak biljaka plavog patlidžana iz devet useva sa četiri različita lokaliteta gajenja ove biljne vrste u Vojvodini. Tokom pregleda, na blizu 40% biljaka plavog patlidžana mogli su se uočiti simptomi u vidu žućkastobeličastih pega raspoređenih po čitavoj površini liske. Prikupljeni uzorci analizirani su na prisustvo virusa mozaika lucerke (alfalfa mosaic virus, AMV), virusa mozaika krastavca (cucumber mosaic virus, CMV) i Y virusa krompira (potato virus Y, PVY) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Serološkim analizama utvrđeno je prisustvo AMV u 80,39% prikupljenih uzoraka. Prisustvo CMV i PVY nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, kao i na klijance plavog patlidžana, čime je potvrđena infe ktivna priroda oboljenja. Prisustvo AMV u biljkama plavog patlidžana je dalje potvrđeno primenom RT-PCR metode (reverzna transkripcija praćena lančanom reakcijom polimeraze) i sekvencioniranjem odabranih izolata korišćenjem specifičnih prajmera CP AMV1 i CP AMV2, koji omogućavaju umnožavanje gena za protein omotača. Filogenetska analiza pokazala je grupisanje srpskih AMV izolata u jasno odvojenu grupu zajedno sa AMV izolatima iz Italije, Hrvatske i prethodno okarakterisanim izolatima iz Srbije. Prema našim saznanjima, ovo je prvi izveštaj o prisustvu virusa mozaika lucerke na plavom patlidžanu u Srbiji. PB - Čačak : Univerzitet u Kragujevcu, Agronomski fakultet u Čačku T2 - Acta Agriculturae Serbica T1 - Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia EP - 39 IS - 51 SP - 33 VL - 26 DO - 10.5937/AASer2151033M ER -
@article{ author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Tamindžić, Gordana and Petrović, Gordana and Vlajić, Slobodan and Stanković, Ivana", year = "2021", abstract = "In the 2018–19 growing season, a total of 51 leaves of eggplant plants grown under field conditions were collected randomly from nine private gardens at four different localities in the Province of Vojvodina. Eggplants with nearly 40% of plants showing bright yellow to white mosaic or mottling of leaves were found throughout the inspected fields (gardens). The collected samples were analyzed for the presence of alfalfa mosaic virus (AMV), cucumber mosaic virus (CMV) and potato virus Y (PVY) using commercial double-antibody sandwich (DAS)-ELISA kits. Serological analysis of eggplant samples revealed the presence of AMV in 80.39% collected samples. None of the analyzed samples was positive for CMV and PVY. The virus was successfully mechanically transmitted to test plants including Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, as well as eggplant seedlings, confirming the infectious nature of the disease. The presence of AMV in eggplants was further verified by reverse transcription–polymerase chain reaction (RT-PCR) and sequencing, using the primers CP AMV1 and CP AMV2 that amplify part of the coat protein (CP) gene. The phylogenetic analysis showed that Serbian AMV isolates grouped into a separate well-supported group together with AMV isolates from Italy, Croatia and previously characterized isolates from Serbia. To our knowledge, this is the first report of AMV infection of eggplant in Serbia., Tokom 2018. i 2019. godine, prikupljen je 51 uzorak biljaka plavog patlidžana iz devet useva sa četiri različita lokaliteta gajenja ove biljne vrste u Vojvodini. Tokom pregleda, na blizu 40% biljaka plavog patlidžana mogli su se uočiti simptomi u vidu žućkastobeličastih pega raspoređenih po čitavoj površini liske. Prikupljeni uzorci analizirani su na prisustvo virusa mozaika lucerke (alfalfa mosaic virus, AMV), virusa mozaika krastavca (cucumber mosaic virus, CMV) i Y virusa krompira (potato virus Y, PVY) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Serološkim analizama utvrđeno je prisustvo AMV u 80,39% prikupljenih uzoraka. Prisustvo CMV i PVY nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, kao i na klijance plavog patlidžana, čime je potvrđena infe ktivna priroda oboljenja. Prisustvo AMV u biljkama plavog patlidžana je dalje potvrđeno primenom RT-PCR metode (reverzna transkripcija praćena lančanom reakcijom polimeraze) i sekvencioniranjem odabranih izolata korišćenjem specifičnih prajmera CP AMV1 i CP AMV2, koji omogućavaju umnožavanje gena za protein omotača. Filogenetska analiza pokazala je grupisanje srpskih AMV izolata u jasno odvojenu grupu zajedno sa AMV izolatima iz Italije, Hrvatske i prethodno okarakterisanim izolatima iz Srbije. Prema našim saznanjima, ovo je prvi izveštaj o prisustvu virusa mozaika lucerke na plavom patlidžanu u Srbiji.", publisher = "Čačak : Univerzitet u Kragujevcu, Agronomski fakultet u Čačku", journal = "Acta Agriculturae Serbica", title = "Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia", pages = "39-33", number = "51", volume = "26", doi = "10.5937/AASer2151033M" }
Milošević, D., Ignjatov, M., Nikolić, Z., Tamindžić, G., Petrović, G., Vlajić, S.,& Stanković, I.. (2021). Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia. in Acta Agriculturae Serbica Čačak : Univerzitet u Kragujevcu, Agronomski fakultet u Čačku., 26(51), 33-39. https://doi.org/10.5937/AASer2151033M
Milošević D, Ignjatov M, Nikolić Z, Tamindžić G, Petrović G, Vlajić S, Stanković I. Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia. in Acta Agriculturae Serbica. 2021;26(51):33-39. doi:10.5937/AASer2151033M .
Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Tamindžić, Gordana, Petrović, Gordana, Vlajić, Slobodan, Stanković, Ivana, "Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia" in Acta Agriculturae Serbica, 26, no. 51 (2021):33-39, https://doi.org/10.5937/AASer2151033M . .