Pollen grain traits of oil species from the Novi Sad collection
Osobine polena uljanih vrsta iz novosadske kolekcije
Apstrakt
The collection of oil species in Novi Sad contains 12 species represented with 1-4 cultivars or landraces. In the continuous work on this collection in the sense of breeding of some of those species and their usage as a source of 'desirable genes' we analyzed pollen grain morphology (shape and size), as well as pollen viability. To determine mentioned pollen traits we used Axiovert 40C microscope together with a software package (AxioVision LE; Rel.4.3.) for measurement of pollen length and width. Pollen viability was determined using a staining method (ALEXANDER, 1969). The results showed that species differ by pollen grain shape (round, egg-shaped, triangular and rod) as well as by shape of exine (thick and spiky, thick to thin). In some species there was a specific number of apertures present (1-11). The size of viable pollen grains ranged from 29,10/12,58μ (coriander) to 176,63/169,94μ (oil gourd), while non-viable pollen grains were always smaller (27,27/10,97μ to 119,62/100,86μ) ...at the same plant species. Pollen viability of most species was around 80%. Lowest pollen viability was found in white flax (56,98%), and the highest in oil pumpkin (91,43%).
Kolekcija uljanih vrsta u Novom Sadu raspolaže sa 12 vrsta zastupljenih sa 1-4 sorte, odnosno lokalne populacije. Za dalji rad na korišćenju ove kolekcije u smislu oplemenjivanja pojedinih vrsta ili korišćenje istih kao izvor 'poželjnih gena' ispitivana je morfologija polenovih zrna (oblik i veličina), kao i vitalnost polena. Za određivanje osobina polena korišćen je mikroskop Axiovert 40C, a za merenje dužine i širine polenovih zrna softver AxioVision LE; Rel.4.3. Vitalnost polena je određena bojenom metodom (ALEXANDER, 1969). Rezultati ispitivanja su pokazali da su se vrste razlikovale po obliku polenovih zrna (okrugla, jajasta, trouglasta i štapičasta) kao i po izgledu egzine (debela i bodljikava, debela do tanka). Za neke vrste je karakteristično postojanje određenog broja septi (1-11). Veličina vitalnih polenovih zrna se kretala od 29,10/12,58μ (korijander) do 176,63/169,94μ (uljana bundeva), dok su sterilna zrna bila uvek sitnija (27,27/10,97μ do 119,62/100/86μ) kod istih biljnih... vrsta. Vitalnost polena kod najvećeg broja vrsta je bila oko 80%. Najnižu vitalnost polena je imao lan beli (56,98%), a najvišu uljana tikva (91,43%).
Ključne reči:
oil species / pollen grain shape and size / pollen viabilityIzvor:
Genetika-Belgrade, 2009, 41, 3, 263-270Izdavač:
- Društvo genetičara Srbije, Beograd
Kolekcije
Institucija/grupa
FiVeRTY - JOUR AU - Atlagić, Jovanka AU - Marjanović-Jeromela, Ana AU - Marinković, Radovan AU - Terzić, Sreten PY - 2009 UR - http://fiver.ifvcns.rs/handle/123456789/662 AB - The collection of oil species in Novi Sad contains 12 species represented with 1-4 cultivars or landraces. In the continuous work on this collection in the sense of breeding of some of those species and their usage as a source of 'desirable genes' we analyzed pollen grain morphology (shape and size), as well as pollen viability. To determine mentioned pollen traits we used Axiovert 40C microscope together with a software package (AxioVision LE; Rel.4.3.) for measurement of pollen length and width. Pollen viability was determined using a staining method (ALEXANDER, 1969). The results showed that species differ by pollen grain shape (round, egg-shaped, triangular and rod) as well as by shape of exine (thick and spiky, thick to thin). In some species there was a specific number of apertures present (1-11). The size of viable pollen grains ranged from 29,10/12,58μ (coriander) to 176,63/169,94μ (oil gourd), while non-viable pollen grains were always smaller (27,27/10,97μ to 119,62/100,86μ) at the same plant species. Pollen viability of most species was around 80%. Lowest pollen viability was found in white flax (56,98%), and the highest in oil pumpkin (91,43%). AB - Kolekcija uljanih vrsta u Novom Sadu raspolaže sa 12 vrsta zastupljenih sa 1-4 sorte, odnosno lokalne populacije. Za dalji rad na korišćenju ove kolekcije u smislu oplemenjivanja pojedinih vrsta ili korišćenje istih kao izvor 'poželjnih gena' ispitivana je morfologija polenovih zrna (oblik i veličina), kao i vitalnost polena. Za određivanje osobina polena korišćen je mikroskop Axiovert 40C, a za merenje dužine i širine polenovih zrna softver AxioVision LE; Rel.4.3. Vitalnost polena je određena bojenom metodom (ALEXANDER, 1969). Rezultati ispitivanja su pokazali da su se vrste razlikovale po obliku polenovih zrna (okrugla, jajasta, trouglasta i štapičasta) kao i po izgledu egzine (debela i bodljikava, debela do tanka). Za neke vrste je karakteristično postojanje određenog broja septi (1-11). Veličina vitalnih polenovih zrna se kretala od 29,10/12,58μ (korijander) do 176,63/169,94μ (uljana bundeva), dok su sterilna zrna bila uvek sitnija (27,27/10,97μ do 119,62/100/86μ) kod istih biljnih vrsta. Vitalnost polena kod najvećeg broja vrsta je bila oko 80%. Najnižu vitalnost polena je imao lan beli (56,98%), a najvišu uljana tikva (91,43%). PB - Društvo genetičara Srbije, Beograd T2 - Genetika-Belgrade T1 - Pollen grain traits of oil species from the Novi Sad collection T1 - Osobine polena uljanih vrsta iz novosadske kolekcije EP - 270 IS - 3 SP - 263 VL - 41 DO - 10.2298/GENSR0903263A ER -
@article{ author = "Atlagić, Jovanka and Marjanović-Jeromela, Ana and Marinković, Radovan and Terzić, Sreten", year = "2009", abstract = "The collection of oil species in Novi Sad contains 12 species represented with 1-4 cultivars or landraces. In the continuous work on this collection in the sense of breeding of some of those species and their usage as a source of 'desirable genes' we analyzed pollen grain morphology (shape and size), as well as pollen viability. To determine mentioned pollen traits we used Axiovert 40C microscope together with a software package (AxioVision LE; Rel.4.3.) for measurement of pollen length and width. Pollen viability was determined using a staining method (ALEXANDER, 1969). The results showed that species differ by pollen grain shape (round, egg-shaped, triangular and rod) as well as by shape of exine (thick and spiky, thick to thin). In some species there was a specific number of apertures present (1-11). The size of viable pollen grains ranged from 29,10/12,58μ (coriander) to 176,63/169,94μ (oil gourd), while non-viable pollen grains were always smaller (27,27/10,97μ to 119,62/100,86μ) at the same plant species. Pollen viability of most species was around 80%. Lowest pollen viability was found in white flax (56,98%), and the highest in oil pumpkin (91,43%)., Kolekcija uljanih vrsta u Novom Sadu raspolaže sa 12 vrsta zastupljenih sa 1-4 sorte, odnosno lokalne populacije. Za dalji rad na korišćenju ove kolekcije u smislu oplemenjivanja pojedinih vrsta ili korišćenje istih kao izvor 'poželjnih gena' ispitivana je morfologija polenovih zrna (oblik i veličina), kao i vitalnost polena. Za određivanje osobina polena korišćen je mikroskop Axiovert 40C, a za merenje dužine i širine polenovih zrna softver AxioVision LE; Rel.4.3. Vitalnost polena je određena bojenom metodom (ALEXANDER, 1969). Rezultati ispitivanja su pokazali da su se vrste razlikovale po obliku polenovih zrna (okrugla, jajasta, trouglasta i štapičasta) kao i po izgledu egzine (debela i bodljikava, debela do tanka). Za neke vrste je karakteristično postojanje određenog broja septi (1-11). Veličina vitalnih polenovih zrna se kretala od 29,10/12,58μ (korijander) do 176,63/169,94μ (uljana bundeva), dok su sterilna zrna bila uvek sitnija (27,27/10,97μ do 119,62/100/86μ) kod istih biljnih vrsta. Vitalnost polena kod najvećeg broja vrsta je bila oko 80%. Najnižu vitalnost polena je imao lan beli (56,98%), a najvišu uljana tikva (91,43%).", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika-Belgrade", title = "Pollen grain traits of oil species from the Novi Sad collection, Osobine polena uljanih vrsta iz novosadske kolekcije", pages = "270-263", number = "3", volume = "41", doi = "10.2298/GENSR0903263A" }
Atlagić, J., Marjanović-Jeromela, A., Marinković, R.,& Terzić, S.. (2009). Pollen grain traits of oil species from the Novi Sad collection. in Genetika-Belgrade Društvo genetičara Srbije, Beograd., 41(3), 263-270. https://doi.org/10.2298/GENSR0903263A
Atlagić J, Marjanović-Jeromela A, Marinković R, Terzić S. Pollen grain traits of oil species from the Novi Sad collection. in Genetika-Belgrade. 2009;41(3):263-270. doi:10.2298/GENSR0903263A .
Atlagić, Jovanka, Marjanović-Jeromela, Ana, Marinković, Radovan, Terzić, Sreten, "Pollen grain traits of oil species from the Novi Sad collection" in Genetika-Belgrade, 41, no. 3 (2009):263-270, https://doi.org/10.2298/GENSR0903263A . .