Effectiveness of species and trichothecene specific primers in monitoring Fusarium graminearum species complex in small grain pea intercropping systems
Аутори
Župunski, VesnaJevtić, Radivoje
Grčak, Milosav
Lalošević, Mirjana
Orbović, Branka
Živanov, Dalibor
Knežević, Desimir
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Tracking the distribution of Fusarium species and the detection of changes in toxin production
provides epidemiological information that is essential for Fusarium head blight (FHB) management.
Members of Fusarium graminearum species complex (FGSC) were characterized using species
and trichothecene-specific primers. Associations between members of the FGSC, cereal crop species
(wheat, rye, triticale, and oat), seeding time (winter and spring), type of cultivation (monocrop and
intercrop) and chemotype grouping were investigated with multiple correspondence analysis and
multiple regression modeling. We found that triticale and oat were more related to isolates classified
into F. graminearum s. lato than with other isolates. In contrast, wheat and rye were more associated
with F. graminearum s. stricto. Cereal crop species affected the frequencies of F. graminearum s. stricto
(p = 0.003) and F. graminearum s. lato (p = 0.08) and unidentified isolates with morphological characteri...stics
like those of FGSC members (p = 0.02). The effectiveness of species-specific primers was 60.3%
(Fg16F/R) and 76.2% (FgrF/FgcR), and the effectiveness of primer sets for the trichothecene genotyping
of the Tri5 and Tri3 genes was 100% and 90.6%, respectively. The decrease in Fusarium-damaged
kernel values in the wheat–pea intercropping system indicated that intercropping systems have the
potential to control FHB.
Кључне речи:
Fusarium / graminearum / species / complex / small / grains / intercropping / sequence / characterized / amplified / region / SCAR / genotyping / trichotheceneИзвор:
Agriculture, 2022, 12, 834-14Издавач:
- Basel : MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200032 (Научни институт за ратарство и повртарство, Нови Сад) (RS-MESTD-inst-2020-200032)
DOI: 10.3390/agriculture12060834
ISSN: 2077-0472
WoS: 00081840600000
Scopus: 2-s2.0-85132306118
Колекције
Институција/група
FiVeRTY - JOUR AU - Župunski, Vesna AU - Jevtić, Radivoje AU - Grčak, Milosav AU - Lalošević, Mirjana AU - Orbović, Branka AU - Živanov, Dalibor AU - Knežević, Desimir PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/3566 AB - Tracking the distribution of Fusarium species and the detection of changes in toxin production provides epidemiological information that is essential for Fusarium head blight (FHB) management. Members of Fusarium graminearum species complex (FGSC) were characterized using species and trichothecene-specific primers. Associations between members of the FGSC, cereal crop species (wheat, rye, triticale, and oat), seeding time (winter and spring), type of cultivation (monocrop and intercrop) and chemotype grouping were investigated with multiple correspondence analysis and multiple regression modeling. We found that triticale and oat were more related to isolates classified into F. graminearum s. lato than with other isolates. In contrast, wheat and rye were more associated with F. graminearum s. stricto. Cereal crop species affected the frequencies of F. graminearum s. stricto (p = 0.003) and F. graminearum s. lato (p = 0.08) and unidentified isolates with morphological characteristics like those of FGSC members (p = 0.02). The effectiveness of species-specific primers was 60.3% (Fg16F/R) and 76.2% (FgrF/FgcR), and the effectiveness of primer sets for the trichothecene genotyping of the Tri5 and Tri3 genes was 100% and 90.6%, respectively. The decrease in Fusarium-damaged kernel values in the wheat–pea intercropping system indicated that intercropping systems have the potential to control FHB. PB - Basel : MDPI T2 - Agriculture T1 - Effectiveness of species and trichothecene specific primers in monitoring Fusarium graminearum species complex in small grain pea intercropping systems EP - 14 SP - 834 VL - 12 DO - 10.3390/agriculture12060834 ER -
@article{ author = "Župunski, Vesna and Jevtić, Radivoje and Grčak, Milosav and Lalošević, Mirjana and Orbović, Branka and Živanov, Dalibor and Knežević, Desimir", year = "2022", abstract = "Tracking the distribution of Fusarium species and the detection of changes in toxin production provides epidemiological information that is essential for Fusarium head blight (FHB) management. Members of Fusarium graminearum species complex (FGSC) were characterized using species and trichothecene-specific primers. Associations between members of the FGSC, cereal crop species (wheat, rye, triticale, and oat), seeding time (winter and spring), type of cultivation (monocrop and intercrop) and chemotype grouping were investigated with multiple correspondence analysis and multiple regression modeling. We found that triticale and oat were more related to isolates classified into F. graminearum s. lato than with other isolates. In contrast, wheat and rye were more associated with F. graminearum s. stricto. Cereal crop species affected the frequencies of F. graminearum s. stricto (p = 0.003) and F. graminearum s. lato (p = 0.08) and unidentified isolates with morphological characteristics like those of FGSC members (p = 0.02). The effectiveness of species-specific primers was 60.3% (Fg16F/R) and 76.2% (FgrF/FgcR), and the effectiveness of primer sets for the trichothecene genotyping of the Tri5 and Tri3 genes was 100% and 90.6%, respectively. The decrease in Fusarium-damaged kernel values in the wheat–pea intercropping system indicated that intercropping systems have the potential to control FHB.", publisher = "Basel : MDPI", journal = "Agriculture", title = "Effectiveness of species and trichothecene specific primers in monitoring Fusarium graminearum species complex in small grain pea intercropping systems", pages = "14-834", volume = "12", doi = "10.3390/agriculture12060834" }
Župunski, V., Jevtić, R., Grčak, M., Lalošević, M., Orbović, B., Živanov, D.,& Knežević, D.. (2022). Effectiveness of species and trichothecene specific primers in monitoring Fusarium graminearum species complex in small grain pea intercropping systems. in Agriculture Basel : MDPI., 12, 834-14. https://doi.org/10.3390/agriculture12060834
Župunski V, Jevtić R, Grčak M, Lalošević M, Orbović B, Živanov D, Knežević D. Effectiveness of species and trichothecene specific primers in monitoring Fusarium graminearum species complex in small grain pea intercropping systems. in Agriculture. 2022;12:834-14. doi:10.3390/agriculture12060834 .
Župunski, Vesna, Jevtić, Radivoje, Grčak, Milosav, Lalošević, Mirjana, Orbović, Branka, Živanov, Dalibor, Knežević, Desimir, "Effectiveness of species and trichothecene specific primers in monitoring Fusarium graminearum species complex in small grain pea intercropping systems" in Agriculture, 12 (2022):834-14, https://doi.org/10.3390/agriculture12060834 . .