Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum)
Samo za registrovane korisnike
2018
Autori
Moreau, CarolHofer, Julie M. I.
Eleouet, Morgane
Sinjushin, Andrey
Ambrose, Mike
Skot, Kirsten
Blackmore, Tina
Swain, Martin
Hegarty, Matthew
Balanza, Vicente
Ferrandiz, Cristina
Ellis, Noel
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata, conferring stipule identity, was known to interact with Stipules reduced (St), which conditions stipule size in pea, but the St gene remained unknown. Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing. We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule. Our approach shows how systematic genome-wide screens can be used successfully for the analysis of traits in specie...s for which whole genome sequences are not available.
Ključne reči:
C2H2 zinc finger / Fast Neutron / leaf / mutant / pea / Pisum sativum / RAD sequencing / stipuleIzvor:
New Phytologist, 2018, 220, 1, 288-299Izdavač:
- New Phytologist Foundation
Kolekcije
Institucija/grupa
FiVeRTY - JOUR AU - Moreau, Carol AU - Hofer, Julie M. I. AU - Eleouet, Morgane AU - Sinjushin, Andrey AU - Ambrose, Mike AU - Skot, Kirsten AU - Blackmore, Tina AU - Swain, Martin AU - Hegarty, Matthew AU - Balanza, Vicente AU - Ferrandiz, Cristina AU - Ellis, Noel PY - 2018 UR - http://fiver.ifvcns.rs/handle/123456789/3497 AB - Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata, conferring stipule identity, was known to interact with Stipules reduced (St), which conditions stipule size in pea, but the St gene remained unknown. Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing. We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule. Our approach shows how systematic genome-wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available. PB - New Phytologist Foundation T2 - New Phytologist T1 - Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum) EP - 299 IS - 1 SP - 288 VL - 220 DO - 10.1111/nph.15286 ER -
@article{ author = "Moreau, Carol and Hofer, Julie M. I. and Eleouet, Morgane and Sinjushin, Andrey and Ambrose, Mike and Skot, Kirsten and Blackmore, Tina and Swain, Martin and Hegarty, Matthew and Balanza, Vicente and Ferrandiz, Cristina and Ellis, Noel", year = "2018", abstract = "Pea (Pisum sativum) is one of relatively few genetically amenable plant species with compound leaves. Pea leaves have a variety of specialized organs: leaflets, tendrils, pulvini and stipules, which enable the identification of mutations that transform or affect distinct parts of the leaf. Characterization of these mutations offers insights into the development and evolution of novel leaf traits. The previously characterized morphological gene Cochleata, conferring stipule identity, was known to interact with Stipules reduced (St), which conditions stipule size in pea, but the St gene remained unknown. Here we analysed Fast Neutron irradiated pea mutants by restriction site associated DNA sequencing. We identified St as a gene encoding a C2H2 zinc finger transcription factor that is regulated by Cochleata. St regulates both cell division and cell expansion in the stipule. Our approach shows how systematic genome-wide screens can be used successfully for the analysis of traits in species for which whole genome sequences are not available.", publisher = "New Phytologist Foundation", journal = "New Phytologist", title = "Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum)", pages = "299-288", number = "1", volume = "220", doi = "10.1111/nph.15286" }
Moreau, C., Hofer, J. M. I., Eleouet, M., Sinjushin, A., Ambrose, M., Skot, K., Blackmore, T., Swain, M., Hegarty, M., Balanza, V., Ferrandiz, C.,& Ellis, N.. (2018). Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum). in New Phytologist New Phytologist Foundation., 220(1), 288-299. https://doi.org/10.1111/nph.15286
Moreau C, Hofer JMI, Eleouet M, Sinjushin A, Ambrose M, Skot K, Blackmore T, Swain M, Hegarty M, Balanza V, Ferrandiz C, Ellis N. Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum). in New Phytologist. 2018;220(1):288-299. doi:10.1111/nph.15286 .
Moreau, Carol, Hofer, Julie M. I., Eleouet, Morgane, Sinjushin, Andrey, Ambrose, Mike, Skot, Kirsten, Blackmore, Tina, Swain, Martin, Hegarty, Matthew, Balanza, Vicente, Ferrandiz, Cristina, Ellis, Noel, "Identification of Stipules reduced, a leaf morphology gene in pea (Pisum sativum)" in New Phytologist, 220, no. 1 (2018):288-299, https://doi.org/10.1111/nph.15286 . .