Assessment of method efficiency for sunflower inoculation with Macrophomina phaseolina: important step in sunflower tolerance testing
Authors
Ćuk, NemanjaCvejić, Sandra
Mladenov, Velimir
Dedić, Boško
Babec, Brankica
Gvozdenac, Sonja
Jocić, Siniša
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The fungal disease, Charcoal rot, caused by Macrophomina phaseolina is becoming one of important pathogen in sunflower production, mostly expressed in areas with high temperatures and dry conditions. Development of tolerant sunflower genotypes is the most reliable way to control this disease. It is important to find proper evaluation method which can provide accurate insight in potential genetic control of tolerance in sunflower. The aim of this work is: to compare two inoculation methods of sunflower with M. phaseolina, to compare the tolerance of inbred lines to this pathogen and to determine which method gives better insight into the inheritance of tolerance in sunflower. The experiment was determined under greenhouse conditions and two methods were used. The Unwounded stem base infection method (USBI), is less aggressive approach where the infection is similar to natural conditions. While for the toothpick method (TM) inoculation it is necessary to puncturing the tissue with the to...othpick and create the entrance for pathogen infection. Four inbred lines were used in research which was created at the Institute of Field and Vegetable Crops, Novi Sad (IFVCNS).
Keywords:
charcoal rot / inbred lines / USBI / toothpick method / fungal disease / Macrophomina phaseolina / sunflower / toleranceSource:
Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja, 2019, 236-236Publisher:
- Belgrade : Serbian Genetic Society
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FiVeRTY - CONF AU - Ćuk, Nemanja AU - Cvejić, Sandra AU - Mladenov, Velimir AU - Dedić, Boško AU - Babec, Brankica AU - Gvozdenac, Sonja AU - Jocić, Siniša PY - 2019 UR - http://fiver.ifvcns.rs/handle/123456789/3074 AB - The fungal disease, Charcoal rot, caused by Macrophomina phaseolina is becoming one of important pathogen in sunflower production, mostly expressed in areas with high temperatures and dry conditions. Development of tolerant sunflower genotypes is the most reliable way to control this disease. It is important to find proper evaluation method which can provide accurate insight in potential genetic control of tolerance in sunflower. The aim of this work is: to compare two inoculation methods of sunflower with M. phaseolina, to compare the tolerance of inbred lines to this pathogen and to determine which method gives better insight into the inheritance of tolerance in sunflower. The experiment was determined under greenhouse conditions and two methods were used. The Unwounded stem base infection method (USBI), is less aggressive approach where the infection is similar to natural conditions. While for the toothpick method (TM) inoculation it is necessary to puncturing the tissue with the toothpick and create the entrance for pathogen infection. Four inbred lines were used in research which was created at the Institute of Field and Vegetable Crops, Novi Sad (IFVCNS). PB - Belgrade : Serbian Genetic Society C3 - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja T1 - Assessment of method efficiency for sunflower inoculation with Macrophomina phaseolina: important step in sunflower tolerance testing EP - 236 SP - 236 UR - https://hdl.handle.net/21.15107/rcub_fiver_3074 ER -
@conference{ author = "Ćuk, Nemanja and Cvejić, Sandra and Mladenov, Velimir and Dedić, Boško and Babec, Brankica and Gvozdenac, Sonja and Jocić, Siniša", year = "2019", abstract = "The fungal disease, Charcoal rot, caused by Macrophomina phaseolina is becoming one of important pathogen in sunflower production, mostly expressed in areas with high temperatures and dry conditions. Development of tolerant sunflower genotypes is the most reliable way to control this disease. It is important to find proper evaluation method which can provide accurate insight in potential genetic control of tolerance in sunflower. The aim of this work is: to compare two inoculation methods of sunflower with M. phaseolina, to compare the tolerance of inbred lines to this pathogen and to determine which method gives better insight into the inheritance of tolerance in sunflower. The experiment was determined under greenhouse conditions and two methods were used. The Unwounded stem base infection method (USBI), is less aggressive approach where the infection is similar to natural conditions. While for the toothpick method (TM) inoculation it is necessary to puncturing the tissue with the toothpick and create the entrance for pathogen infection. Four inbred lines were used in research which was created at the Institute of Field and Vegetable Crops, Novi Sad (IFVCNS).", publisher = "Belgrade : Serbian Genetic Society", journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja", title = "Assessment of method efficiency for sunflower inoculation with Macrophomina phaseolina: important step in sunflower tolerance testing", pages = "236-236", url = "https://hdl.handle.net/21.15107/rcub_fiver_3074" }
Ćuk, N., Cvejić, S., Mladenov, V., Dedić, B., Babec, B., Gvozdenac, S.,& Jocić, S.. (2019). Assessment of method efficiency for sunflower inoculation with Macrophomina phaseolina: important step in sunflower tolerance testing. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja Belgrade : Serbian Genetic Society., 236-236. https://hdl.handle.net/21.15107/rcub_fiver_3074
Ćuk N, Cvejić S, Mladenov V, Dedić B, Babec B, Gvozdenac S, Jocić S. Assessment of method efficiency for sunflower inoculation with Macrophomina phaseolina: important step in sunflower tolerance testing. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:236-236. https://hdl.handle.net/21.15107/rcub_fiver_3074 .
Ćuk, Nemanja, Cvejić, Sandra, Mladenov, Velimir, Dedić, Boško, Babec, Brankica, Gvozdenac, Sonja, Jocić, Siniša, "Assessment of method efficiency for sunflower inoculation with Macrophomina phaseolina: important step in sunflower tolerance testing" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):236-236, https://hdl.handle.net/21.15107/rcub_fiver_3074 .