Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower
Authors
Radanović, AleksandraHorn, Renate
Sprycha, Yves
Hamrit, Sonia
Jocković, Milan
Cvejić, Sandra
Jocić, Siniša
Miklič, Vladimir
Miladinović, Dragana
Conference object (Published version)
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Sunflower is the second most common crop among hybrids, worldwide. Development of cytoplasmic male sterile and fertility restoration lines enables creation of hybrids. Sunflower hybrid breeding most frequently relies on the combination of the CMS PET1 cytoplasm and the fertility restoration gene Rf1. Use of molecular markers can accelerate creation of restorer lines. Previous work on this subject included mapping of the Rf1 gene on chromosome 13 and development of bacterial artificial chromosome (BAC) libraries for the restorer line RHA325 and the maintainer line HA383, which enabled positioning of BAC clones surrounding the Rf1 gene in the cross RHA325 x HA342. In this study, BAC-end sequences were used to derive primers in order to amplify selected regions from RHA325 and the maintainer line, HA342. While the majority of primer combinations were monomorphic, some were polymorphic between RHA325 and HA342. Previously reported markers for detection of Rf1 gene and newly developed ones ...based on BAC-end sequences were further tested on hybrids and its components (A, B and R lines) created at the Institute of Field and Vegetable Crops, Novi Sad.
Keywords:
Helianthus annuus / sunflower / marker assisted selection / MAS / fertility restoration / hybrids / breedingSource:
Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja, 2019, 186-186Publisher:
- Belgrade : Serbian Genetic Society
Funding / projects:
- Bilateral project Germany-Serbia (No. 451-03-01732/2017-09/3 and project code 57393592)
- Deutsche Forschungsgemeinschaft (DFG) projects HO 1593/5-1 and HO 1593/5-2
- Development of new varieties and production technology improvement of oil crops for different purposes (RS-MESTD-Technological Development (TD or TR)-31025)
- COST Action CA18111: Genome editing in plants - a technology with transformative potential
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FiVeRTY - CONF AU - Radanović, Aleksandra AU - Horn, Renate AU - Sprycha, Yves AU - Hamrit, Sonia AU - Jocković, Milan AU - Cvejić, Sandra AU - Jocić, Siniša AU - Miklič, Vladimir AU - Miladinović, Dragana PY - 2019 UR - http://fiver.ifvcns.rs/handle/123456789/3063 AB - Sunflower is the second most common crop among hybrids, worldwide. Development of cytoplasmic male sterile and fertility restoration lines enables creation of hybrids. Sunflower hybrid breeding most frequently relies on the combination of the CMS PET1 cytoplasm and the fertility restoration gene Rf1. Use of molecular markers can accelerate creation of restorer lines. Previous work on this subject included mapping of the Rf1 gene on chromosome 13 and development of bacterial artificial chromosome (BAC) libraries for the restorer line RHA325 and the maintainer line HA383, which enabled positioning of BAC clones surrounding the Rf1 gene in the cross RHA325 x HA342. In this study, BAC-end sequences were used to derive primers in order to amplify selected regions from RHA325 and the maintainer line, HA342. While the majority of primer combinations were monomorphic, some were polymorphic between RHA325 and HA342. Previously reported markers for detection of Rf1 gene and newly developed ones based on BAC-end sequences were further tested on hybrids and its components (A, B and R lines) created at the Institute of Field and Vegetable Crops, Novi Sad. PB - Belgrade : Serbian Genetic Society C3 - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja T1 - Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower EP - 186 SP - 186 UR - https://hdl.handle.net/21.15107/rcub_fiver_3063 ER -
@conference{ author = "Radanović, Aleksandra and Horn, Renate and Sprycha, Yves and Hamrit, Sonia and Jocković, Milan and Cvejić, Sandra and Jocić, Siniša and Miklič, Vladimir and Miladinović, Dragana", year = "2019", abstract = "Sunflower is the second most common crop among hybrids, worldwide. Development of cytoplasmic male sterile and fertility restoration lines enables creation of hybrids. Sunflower hybrid breeding most frequently relies on the combination of the CMS PET1 cytoplasm and the fertility restoration gene Rf1. Use of molecular markers can accelerate creation of restorer lines. Previous work on this subject included mapping of the Rf1 gene on chromosome 13 and development of bacterial artificial chromosome (BAC) libraries for the restorer line RHA325 and the maintainer line HA383, which enabled positioning of BAC clones surrounding the Rf1 gene in the cross RHA325 x HA342. In this study, BAC-end sequences were used to derive primers in order to amplify selected regions from RHA325 and the maintainer line, HA342. While the majority of primer combinations were monomorphic, some were polymorphic between RHA325 and HA342. Previously reported markers for detection of Rf1 gene and newly developed ones based on BAC-end sequences were further tested on hybrids and its components (A, B and R lines) created at the Institute of Field and Vegetable Crops, Novi Sad.", publisher = "Belgrade : Serbian Genetic Society", journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja", title = "Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower", pages = "186-186", url = "https://hdl.handle.net/21.15107/rcub_fiver_3063" }
Radanović, A., Horn, R., Sprycha, Y., Hamrit, S., Jocković, M., Cvejić, S., Jocić, S., Miklič, V.,& Miladinović, D.. (2019). Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja Belgrade : Serbian Genetic Society., 186-186. https://hdl.handle.net/21.15107/rcub_fiver_3063
Radanović A, Horn R, Sprycha Y, Hamrit S, Jocković M, Cvejić S, Jocić S, Miklič V, Miladinović D. Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:186-186. https://hdl.handle.net/21.15107/rcub_fiver_3063 .
Radanović, Aleksandra, Horn, Renate, Sprycha, Yves, Hamrit, Sonia, Jocković, Milan, Cvejić, Sandra, Jocić, Siniša, Miklič, Vladimir, Miladinović, Dragana, "Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):186-186, https://hdl.handle.net/21.15107/rcub_fiver_3063 .