Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)
2013
Autori
Jovanović, ŽivkoStanisavljević, Nemanja
Miljuš-Đukić, Jovanka
Mikić, Aleksandar
Radović, Svetlana
Ćupina, Branko
Maksimović, Vesna
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at posttranscriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that that could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame could reflect the changes as closely as possible those during water stress cause visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. On the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) was not changed ...in roots and shoots of water-stressed plants, compared to control-well hydrated plants. This suggests that COX5b is not target of miR398, or that its expression is regulated by some other mechanism. P1B-ATPase expression increased during water deficit only in shoots of pea; in the roots there was no changes in expression. Our results might help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.
Ključne reči:
pea / Pisum sativum / miR398 / miR408 / gene expression / dehydration / miRNAs / water deficitIzvor:
Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad, 2013, 153-153Izdavač:
- International Legume Society
- Novi Sad : Institute of Field and Vegetable Crops
Finansiranje / projekti:
- Povećanje tržišnog značaja krmnih biljaka oplemenjivanjem i optimizacijom tehnologije proizvodnje semena (RS-MESTD-Technological Development (TD or TR)-31024)
- Molekularni mehanizmi odgovora biljaka na abiotički stres-uloga transkripcionih faktora i malih RNK i analiza genetičkog diverziteta biljnih kultura od interesa za poljoprivredu i biotehnologiju (RS-MESTD-Basic Research (BR or ON)-173005)
Kolekcije
Institucija/grupa
FiVeRTY - CONF AU - Jovanović, Živko AU - Stanisavljević, Nemanja AU - Miljuš-Đukić, Jovanka AU - Mikić, Aleksandar AU - Radović, Svetlana AU - Ćupina, Branko AU - Maksimović, Vesna PY - 2013 UR - http://fiver.ifvcns.rs/handle/123456789/3018 AB - MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at posttranscriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that that could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame could reflect the changes as closely as possible those during water stress cause visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. On the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) was not changed in roots and shoots of water-stressed plants, compared to control-well hydrated plants. This suggests that COX5b is not target of miR398, or that its expression is regulated by some other mechanism. P1B-ATPase expression increased during water deficit only in shoots of pea; in the roots there was no changes in expression. Our results might help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit. PB - International Legume Society PB - Novi Sad : Institute of Field and Vegetable Crops C3 - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad T1 - Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.) EP - 153 SP - 153 UR - https://hdl.handle.net/21.15107/rcub_fiver_3018 ER -
@conference{ author = "Jovanović, Živko and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Mikić, Aleksandar and Radović, Svetlana and Ćupina, Branko and Maksimović, Vesna", year = "2013", abstract = "MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at posttranscriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that that could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame could reflect the changes as closely as possible those during water stress cause visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. On the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) was not changed in roots and shoots of water-stressed plants, compared to control-well hydrated plants. This suggests that COX5b is not target of miR398, or that its expression is regulated by some other mechanism. P1B-ATPase expression increased during water deficit only in shoots of pea; in the roots there was no changes in expression. Our results might help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.", publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops", journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad", title = "Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)", pages = "153-153", url = "https://hdl.handle.net/21.15107/rcub_fiver_3018" }
Jovanović, Ž., Stanisavljević, N., Miljuš-Đukić, J., Mikić, A., Radović, S., Ćupina, B.,& Maksimović, V.. (2013). Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad International Legume Society., 153-153. https://hdl.handle.net/21.15107/rcub_fiver_3018
Jovanović Ž, Stanisavljević N, Miljuš-Đukić J, Mikić A, Radović S, Ćupina B, Maksimović V. Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:153-153. https://hdl.handle.net/21.15107/rcub_fiver_3018 .
Jovanović, Živko, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Mikić, Aleksandar, Radović, Svetlana, Ćupina, Branko, Maksimović, Vesna, "Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):153-153, https://hdl.handle.net/21.15107/rcub_fiver_3018 .