Genetic diversity of Orobanche cumana populations in Serbia
Authors
Ivanović, Žarko![](/themes/MirageFiVeR/images/orcid.png)
Marisavljević, Dragana
![](/themes/MirageFiVeR/images/orcid.png)
Marinković, Radovan
Mitrović, Petar
![](/themes/MirageFiVeR/images/orcid.png)
Blagojević, Jovana
Nikolić, Ivan
Pavlović, Danijela
Article (Published version)
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In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding th...e evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.
Keywords:
intrapopulation variation / phylogeny / RAPD / rbcL / sunflower / broomrape / Orobanche cumanaSource:
The Plant Pathology Journal, 2021, 37, 6, 512-520Publisher:
- Korean Society of Plant Pathology
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-MESTD-inst-2020-200010)
DOI: 10.5423/PPJ.OA.04.2021.0066
ISSN: 1598-2254; 2093-9280
WoS: 000727716300002
Scopus: 2-s2.0-85120936066
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FiVeRTY - JOUR AU - Ivanović, Žarko AU - Marisavljević, Dragana AU - Marinković, Radovan AU - Mitrović, Petar AU - Blagojević, Jovana AU - Nikolić, Ivan AU - Pavlović, Danijela PY - 2021 UR - http://fiver.ifvcns.rs/handle/123456789/2973 AB - In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control. PB - Korean Society of Plant Pathology T2 - The Plant Pathology Journal T1 - Genetic diversity of Orobanche cumana populations in Serbia EP - 520 IS - 6 SP - 512 VL - 37 DO - 10.5423/PPJ.OA.04.2021.0066 ER -
@article{ author = "Ivanović, Žarko and Marisavljević, Dragana and Marinković, Radovan and Mitrović, Petar and Blagojević, Jovana and Nikolić, Ivan and Pavlović, Danijela", year = "2021", abstract = "In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.", publisher = "Korean Society of Plant Pathology", journal = "The Plant Pathology Journal", title = "Genetic diversity of Orobanche cumana populations in Serbia", pages = "520-512", number = "6", volume = "37", doi = "10.5423/PPJ.OA.04.2021.0066" }
Ivanović, Ž., Marisavljević, D., Marinković, R., Mitrović, P., Blagojević, J., Nikolić, I.,& Pavlović, D.. (2021). Genetic diversity of Orobanche cumana populations in Serbia. in The Plant Pathology Journal Korean Society of Plant Pathology., 37(6), 512-520. https://doi.org/10.5423/PPJ.OA.04.2021.0066
Ivanović Ž, Marisavljević D, Marinković R, Mitrović P, Blagojević J, Nikolić I, Pavlović D. Genetic diversity of Orobanche cumana populations in Serbia. in The Plant Pathology Journal. 2021;37(6):512-520. doi:10.5423/PPJ.OA.04.2021.0066 .
Ivanović, Žarko, Marisavljević, Dragana, Marinković, Radovan, Mitrović, Petar, Blagojević, Jovana, Nikolić, Ivan, Pavlović, Danijela, "Genetic diversity of Orobanche cumana populations in Serbia" in The Plant Pathology Journal, 37, no. 6 (2021):512-520, https://doi.org/10.5423/PPJ.OA.04.2021.0066 . .