Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina
Authorized Users Only
2022
Authors
Ćuk, NemanjaCvejić, Sandra
Mladenov, Velimir
Miladinović, Dragana
Babec, Brankica
Jocić, Siniša
Dedić, Boško
Article (Published version)
Metadata
Show full item recordAbstract
The fast adaptation to different growing conditions of a fungus Macrophomina phaseolina, led to its becoming one of the sunflower (Helianthus annuus L.) disease causal agents in regions with a temperate climate. Methods currently used to determine sunflower resistance require laborious manual inoculation and confirmation of pathogen appearance, due to the late stage of testing. The paper proposes a cut-stem method for inoculating sunflower plants in the controlled conditions and the possibility of early-stage disease evaluation. A set of 15 sunflower inbred lines was inoculated using M. phaselolina isolate in the growth chamber and the obtained data were analysed using Cut-stem Disease Severity (CSDS) and compared with disease severity obtained from field experiments using traditional inoculation methods (toothpick, Unwounded Stem Base Inoculation (USBI) and non-inoculated plants). The results showed that, based on CSDS, inbred lines infected with the cut-stem inoculation method signif...icantly differed regarding resistance to M. phaseolina. None of the inbred lines exhibited complete resistance but three lines could be proposed as a source of resistance to this pathogen. Ranking of inbred lines which was based on resistance to M.phaseolina was similar in all inoculation methods and in non-inoculated plants. There were highly significant correlations between the values obtained from growth chamber experiment and disease severity scores from field evaluations. Thus, the obtained results indicate that the cut-stem method could potentially complement field testing methods and be valuable tool in sunflower breeding for resistance to M. phaseolina.
Keywords:
charcoal rot / diseases / disease progress / disease severity / progress / severity / early-stage evaluation / early evaluation / inbred lines / inoculation / sunflower / Macrophomina phaseolina / cut-stem inoculationSource:
Phytoparasitica, 2022Publisher:
- Springer
Funding / projects:
- SmartSun - Creating climate smart sunflower for future challenges (RS-ScienceFundRS-Ideje-7732457)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-MESTD-inst-2020-200032)
- Climate Crops - Centre of Excellence for Innovations in Breeding of Climate-Resilient Crops, Institute of Field and Vegetable Crops
Note:
- The accepted peer-reviewed version is available here http://fiver.ifvcns.rs/handle/123456789/2971
- Online access to this article has been shared by the authors via Springer Nature SharedIt: https://rdcu.be/cSBoz
DOI: 10.1007/s12600-022-01015-0
ISSN: 0334-2123; 1876-7184
WoS: 000830971200001
Scopus: 2-s2.0-85135253438
Collections
Institution/Community
FiVeRTY - JOUR AU - Ćuk, Nemanja AU - Cvejić, Sandra AU - Mladenov, Velimir AU - Miladinović, Dragana AU - Babec, Brankica AU - Jocić, Siniša AU - Dedić, Boško PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/2970 AB - The fast adaptation to different growing conditions of a fungus Macrophomina phaseolina, led to its becoming one of the sunflower (Helianthus annuus L.) disease causal agents in regions with a temperate climate. Methods currently used to determine sunflower resistance require laborious manual inoculation and confirmation of pathogen appearance, due to the late stage of testing. The paper proposes a cut-stem method for inoculating sunflower plants in the controlled conditions and the possibility of early-stage disease evaluation. A set of 15 sunflower inbred lines was inoculated using M. phaselolina isolate in the growth chamber and the obtained data were analysed using Cut-stem Disease Severity (CSDS) and compared with disease severity obtained from field experiments using traditional inoculation methods (toothpick, Unwounded Stem Base Inoculation (USBI) and non-inoculated plants). The results showed that, based on CSDS, inbred lines infected with the cut-stem inoculation method significantly differed regarding resistance to M. phaseolina. None of the inbred lines exhibited complete resistance but three lines could be proposed as a source of resistance to this pathogen. Ranking of inbred lines which was based on resistance to M.phaseolina was similar in all inoculation methods and in non-inoculated plants. There were highly significant correlations between the values obtained from growth chamber experiment and disease severity scores from field evaluations. Thus, the obtained results indicate that the cut-stem method could potentially complement field testing methods and be valuable tool in sunflower breeding for resistance to M. phaseolina. PB - Springer T2 - Phytoparasitica T1 - Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina DO - 10.1007/s12600-022-01015-0 ER -
@article{ author = "Ćuk, Nemanja and Cvejić, Sandra and Mladenov, Velimir and Miladinović, Dragana and Babec, Brankica and Jocić, Siniša and Dedić, Boško", year = "2022", abstract = "The fast adaptation to different growing conditions of a fungus Macrophomina phaseolina, led to its becoming one of the sunflower (Helianthus annuus L.) disease causal agents in regions with a temperate climate. Methods currently used to determine sunflower resistance require laborious manual inoculation and confirmation of pathogen appearance, due to the late stage of testing. The paper proposes a cut-stem method for inoculating sunflower plants in the controlled conditions and the possibility of early-stage disease evaluation. A set of 15 sunflower inbred lines was inoculated using M. phaselolina isolate in the growth chamber and the obtained data were analysed using Cut-stem Disease Severity (CSDS) and compared with disease severity obtained from field experiments using traditional inoculation methods (toothpick, Unwounded Stem Base Inoculation (USBI) and non-inoculated plants). The results showed that, based on CSDS, inbred lines infected with the cut-stem inoculation method significantly differed regarding resistance to M. phaseolina. None of the inbred lines exhibited complete resistance but three lines could be proposed as a source of resistance to this pathogen. Ranking of inbred lines which was based on resistance to M.phaseolina was similar in all inoculation methods and in non-inoculated plants. There were highly significant correlations between the values obtained from growth chamber experiment and disease severity scores from field evaluations. Thus, the obtained results indicate that the cut-stem method could potentially complement field testing methods and be valuable tool in sunflower breeding for resistance to M. phaseolina.", publisher = "Springer", journal = "Phytoparasitica", title = "Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina", doi = "10.1007/s12600-022-01015-0" }
Ćuk, N., Cvejić, S., Mladenov, V., Miladinović, D., Babec, B., Jocić, S.,& Dedić, B.. (2022). Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina. in Phytoparasitica Springer.. https://doi.org/10.1007/s12600-022-01015-0
Ćuk N, Cvejić S, Mladenov V, Miladinović D, Babec B, Jocić S, Dedić B. Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina. in Phytoparasitica. 2022;. doi:10.1007/s12600-022-01015-0 .
Ćuk, Nemanja, Cvejić, Sandra, Mladenov, Velimir, Miladinović, Dragana, Babec, Brankica, Jocić, Siniša, Dedić, Boško, "Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina" in Phytoparasitica (2022), https://doi.org/10.1007/s12600-022-01015-0 . .