Botrytis cinerea as causal agent of sunflower seed grey mould
Аутори
Ignjatov, MajaMilošević, Dragana
Miklič, Vladimir
Dedić, Boško
Tamindžić, Gordana
Bjelić, Dragana
Ivanović, Žarko
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Sunflower (Helianthus annuus L.) is one of the most important oil crops worldwide, including Serbia, where it is grown on about 200,000 ha with an average yield of 3 t/ha. Botrytis cinerea Pers. (1794) regularly occurs on sunflower seeds associated with the phenology of the host plant. However, sunflower seed is often affected by the Rhizopus spp., which causes similar symptoms on seed and seedlings as B. cinerea, and it is important to reliably differentiate those species based on morphological and molecular characteristics. The method used in this study for the detection of B. cinerea is described by ISTA method 7-003. The main task of this study was to isolate the causal agent of grey mould and rot of sunflower seed and seedlings. Infected seeds were covered with overgrown dark-grey mycelia with sporulation. In order to isolate the pathogen, the infected seed was transferred onto potato dextrose agar (PDA) and incubated for 7 days at 20°C. For morphological identification, 11 isolat...es were single-spored and subcultured onto PDA. Strains formed straight, noddy conidiophores, branched at the top and resembling a cluster with conidia. Conidia were ovoid or elliptical avg. 11.6 × 7.5 µm in size.
Кључне речи:
Botrytis cinerea / sunflower / seeds / molecular identification / ITS / sequencingИзвор:
Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia, 2022, 209-209Издавач:
- Paris : International Sunflower Association
- Novi Sad : Institute of Field and Vegetable Crops
Финансирање / пројекти:
- Развој нових сорти и побољшање технологија производње уљаних биљних врста за различите намене (RS-MESTD-Technological Development (TD or TR)-31025)
Колекције
Институција/група
FiVeRTY - CONF AU - Ignjatov, Maja AU - Milošević, Dragana AU - Miklič, Vladimir AU - Dedić, Boško AU - Tamindžić, Gordana AU - Bjelić, Dragana AU - Ivanović, Žarko PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/2897 AB - Sunflower (Helianthus annuus L.) is one of the most important oil crops worldwide, including Serbia, where it is grown on about 200,000 ha with an average yield of 3 t/ha. Botrytis cinerea Pers. (1794) regularly occurs on sunflower seeds associated with the phenology of the host plant. However, sunflower seed is often affected by the Rhizopus spp., which causes similar symptoms on seed and seedlings as B. cinerea, and it is important to reliably differentiate those species based on morphological and molecular characteristics. The method used in this study for the detection of B. cinerea is described by ISTA method 7-003. The main task of this study was to isolate the causal agent of grey mould and rot of sunflower seed and seedlings. Infected seeds were covered with overgrown dark-grey mycelia with sporulation. In order to isolate the pathogen, the infected seed was transferred onto potato dextrose agar (PDA) and incubated for 7 days at 20°C. For morphological identification, 11 isolates were single-spored and subcultured onto PDA. Strains formed straight, noddy conidiophores, branched at the top and resembling a cluster with conidia. Conidia were ovoid or elliptical avg. 11.6 × 7.5 µm in size. PB - Paris : International Sunflower Association PB - Novi Sad : Institute of Field and Vegetable Crops C3 - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia T1 - Botrytis cinerea as causal agent of sunflower seed grey mould EP - 209 SP - 209 UR - https://hdl.handle.net/21.15107/rcub_fiver_2897 ER -
@conference{ author = "Ignjatov, Maja and Milošević, Dragana and Miklič, Vladimir and Dedić, Boško and Tamindžić, Gordana and Bjelić, Dragana and Ivanović, Žarko", year = "2022", abstract = "Sunflower (Helianthus annuus L.) is one of the most important oil crops worldwide, including Serbia, where it is grown on about 200,000 ha with an average yield of 3 t/ha. Botrytis cinerea Pers. (1794) regularly occurs on sunflower seeds associated with the phenology of the host plant. However, sunflower seed is often affected by the Rhizopus spp., which causes similar symptoms on seed and seedlings as B. cinerea, and it is important to reliably differentiate those species based on morphological and molecular characteristics. The method used in this study for the detection of B. cinerea is described by ISTA method 7-003. The main task of this study was to isolate the causal agent of grey mould and rot of sunflower seed and seedlings. Infected seeds were covered with overgrown dark-grey mycelia with sporulation. In order to isolate the pathogen, the infected seed was transferred onto potato dextrose agar (PDA) and incubated for 7 days at 20°C. For morphological identification, 11 isolates were single-spored and subcultured onto PDA. Strains formed straight, noddy conidiophores, branched at the top and resembling a cluster with conidia. Conidia were ovoid or elliptical avg. 11.6 × 7.5 µm in size.", publisher = "Paris : International Sunflower Association, Novi Sad : Institute of Field and Vegetable Crops", journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia", title = "Botrytis cinerea as causal agent of sunflower seed grey mould", pages = "209-209", url = "https://hdl.handle.net/21.15107/rcub_fiver_2897" }
Ignjatov, M., Milošević, D., Miklič, V., Dedić, B., Tamindžić, G., Bjelić, D.,& Ivanović, Ž.. (2022). Botrytis cinerea as causal agent of sunflower seed grey mould. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia Paris : International Sunflower Association., 209-209. https://hdl.handle.net/21.15107/rcub_fiver_2897
Ignjatov M, Milošević D, Miklič V, Dedić B, Tamindžić G, Bjelić D, Ivanović Ž. Botrytis cinerea as causal agent of sunflower seed grey mould. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia. 2022;:209-209. https://hdl.handle.net/21.15107/rcub_fiver_2897 .
Ignjatov, Maja, Milošević, Dragana, Miklič, Vladimir, Dedić, Boško, Tamindžić, Gordana, Bjelić, Dragana, Ivanović, Žarko, "Botrytis cinerea as causal agent of sunflower seed grey mould" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia (2022):209-209, https://hdl.handle.net/21.15107/rcub_fiver_2897 .