In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract
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2021
Authors
Aćimović, MilicaŠeregelj, Vanja
Šovljanski, Olja
Tumbas Šaponjac, Vesna
Švarc Gajić, Jaroslava
Brezo-Borjan, Tanja
Pezo, Lato
Article (Published version)
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As the main goal of this study was the verification of Satureja kitaibelii Wierzb. ex Heuff. in form of the crude extract obtained by subcritical water extraction (SWE) technique in terms of polyphenolic composition, in vitro antioxidant capacity, antihyperglycemic, and anti-inflammatory activities as well as an antimicrobial effect against 10 microorganisms. HPLC analysis showed the presence of ten compounds in a total concentration of 89.33 mg/g of the SWE extract, and demonstrated that subcritical water polarity was the most selective for extracting syringic and caffeic acid, as well as epicatechin (37.88 18.06, and 10.04 mg/g, respectively), while flavonoids like rutin, luteolin, and apigenin were present in low concentration (<2 mg/g). Results of antioxidant activity assays revealed that superoxide anion and ABTS•+ radical models expressed the highest scavenging abilities (437.35 and 412.09 μmol TE/g, respectively), followed by lipid radicals (69.61 μmol TE/g), and DPPH• (32.28 μm...ol TE/g), while in the case of ferric ion reducing ability was noted a high value (118.19 μmol TE/g). S. kitaibelii SWE was less efficient in the antihyperglycemic assay (AhgA = 30.72 %), but the high antiinflammatory activity of S. kitaibelii SWE was observed (30.35 %). The antimicrobial activity was demonstrated against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes as well as Saccharomyces cerevisiae, Candida albicans, and Aspergillus brasiliensis through antimicrobial screening, minimal inhibitory concentration, and pharmacodynamics study.
Keywords:
Subcritical water extraction / Satureja kitaibelii / Phytochemical composition / Antioxidant capacity / Antimicrobial activitySource:
Industrial Crops and Products, 2021, 169, 113672-Publisher:
- Elsevier
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-MESTD-inst-2020-200032)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200134 (University of Novi Sad, Faculty of Technology) (RS-MESTD-inst-2020-200134)
DOI: 10.1016/j.indcrop.2021.113672
ISSN: 0926-6690
WoS: 000670204600001
Scopus: 2-s2.0-85106504737
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FiVeRTY - JOUR AU - Aćimović, Milica AU - Šeregelj, Vanja AU - Šovljanski, Olja AU - Tumbas Šaponjac, Vesna AU - Švarc Gajić, Jaroslava AU - Brezo-Borjan, Tanja AU - Pezo, Lato PY - 2021 UR - http://fiver.ifvcns.rs/handle/123456789/2196 AB - As the main goal of this study was the verification of Satureja kitaibelii Wierzb. ex Heuff. in form of the crude extract obtained by subcritical water extraction (SWE) technique in terms of polyphenolic composition, in vitro antioxidant capacity, antihyperglycemic, and anti-inflammatory activities as well as an antimicrobial effect against 10 microorganisms. HPLC analysis showed the presence of ten compounds in a total concentration of 89.33 mg/g of the SWE extract, and demonstrated that subcritical water polarity was the most selective for extracting syringic and caffeic acid, as well as epicatechin (37.88 18.06, and 10.04 mg/g, respectively), while flavonoids like rutin, luteolin, and apigenin were present in low concentration (<2 mg/g). Results of antioxidant activity assays revealed that superoxide anion and ABTS•+ radical models expressed the highest scavenging abilities (437.35 and 412.09 μmol TE/g, respectively), followed by lipid radicals (69.61 μmol TE/g), and DPPH• (32.28 μmol TE/g), while in the case of ferric ion reducing ability was noted a high value (118.19 μmol TE/g). S. kitaibelii SWE was less efficient in the antihyperglycemic assay (AhgA = 30.72 %), but the high antiinflammatory activity of S. kitaibelii SWE was observed (30.35 %). The antimicrobial activity was demonstrated against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes as well as Saccharomyces cerevisiae, Candida albicans, and Aspergillus brasiliensis through antimicrobial screening, minimal inhibitory concentration, and pharmacodynamics study. PB - Elsevier T2 - Industrial Crops and Products T1 - In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract SP - 113672 VL - 169 DO - 10.1016/j.indcrop.2021.113672 ER -
@article{ author = "Aćimović, Milica and Šeregelj, Vanja and Šovljanski, Olja and Tumbas Šaponjac, Vesna and Švarc Gajić, Jaroslava and Brezo-Borjan, Tanja and Pezo, Lato", year = "2021", abstract = "As the main goal of this study was the verification of Satureja kitaibelii Wierzb. ex Heuff. in form of the crude extract obtained by subcritical water extraction (SWE) technique in terms of polyphenolic composition, in vitro antioxidant capacity, antihyperglycemic, and anti-inflammatory activities as well as an antimicrobial effect against 10 microorganisms. HPLC analysis showed the presence of ten compounds in a total concentration of 89.33 mg/g of the SWE extract, and demonstrated that subcritical water polarity was the most selective for extracting syringic and caffeic acid, as well as epicatechin (37.88 18.06, and 10.04 mg/g, respectively), while flavonoids like rutin, luteolin, and apigenin were present in low concentration (<2 mg/g). Results of antioxidant activity assays revealed that superoxide anion and ABTS•+ radical models expressed the highest scavenging abilities (437.35 and 412.09 μmol TE/g, respectively), followed by lipid radicals (69.61 μmol TE/g), and DPPH• (32.28 μmol TE/g), while in the case of ferric ion reducing ability was noted a high value (118.19 μmol TE/g). S. kitaibelii SWE was less efficient in the antihyperglycemic assay (AhgA = 30.72 %), but the high antiinflammatory activity of S. kitaibelii SWE was observed (30.35 %). The antimicrobial activity was demonstrated against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes as well as Saccharomyces cerevisiae, Candida albicans, and Aspergillus brasiliensis through antimicrobial screening, minimal inhibitory concentration, and pharmacodynamics study.", publisher = "Elsevier", journal = "Industrial Crops and Products", title = "In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract", pages = "113672", volume = "169", doi = "10.1016/j.indcrop.2021.113672" }
Aćimović, M., Šeregelj, V., Šovljanski, O., Tumbas Šaponjac, V., Švarc Gajić, J., Brezo-Borjan, T.,& Pezo, L.. (2021). In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract. in Industrial Crops and Products Elsevier., 169, 113672. https://doi.org/10.1016/j.indcrop.2021.113672
Aćimović M, Šeregelj V, Šovljanski O, Tumbas Šaponjac V, Švarc Gajić J, Brezo-Borjan T, Pezo L. In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract. in Industrial Crops and Products. 2021;169:113672. doi:10.1016/j.indcrop.2021.113672 .
Aćimović, Milica, Šeregelj, Vanja, Šovljanski, Olja, Tumbas Šaponjac, Vesna, Švarc Gajić, Jaroslava, Brezo-Borjan, Tanja, Pezo, Lato, "In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract" in Industrial Crops and Products, 169 (2021):113672, https://doi.org/10.1016/j.indcrop.2021.113672 . .