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Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia
Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji
dc.creator | Milošević, Dragana | |
dc.creator | Ignjatov, Maja | |
dc.creator | Marjanović-Jeromela, Ana | |
dc.creator | Stanković, Ivana | |
dc.creator | Nikolić, Zorica | |
dc.creator | Tamindžić, Gordana | |
dc.creator | Krstić, Branka | |
dc.date.accessioned | 2021-04-26T19:46:32Z | |
dc.date.available | 2021-04-26T19:46:32Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1821-3944 | |
dc.identifier.uri | http://fiver.ifvcns.rs/handle/123456789/1896 | |
dc.description.abstract | In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank. | en |
dc.description.abstract | Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka. | sr |
dc.publisher | Institut za ratarstvo i povrtarstvo, Novi Sad | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31025/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31030/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Ratarstvo i povrtarstvo / Field and Vegetable Crops Research | |
dc.subject | DAS-ELTSA | en |
dc.subject | mustards | en |
dc.subject | phylogenetic analysis | en |
dc.subject | RT-PCR | en |
dc.subject | Turnip yellows virus | en |
dc.subject | DAS-ELISA | sr |
dc.subject | filogenetska analiza | sr |
dc.subject | RT-PCR | sr |
dc.subject | slačica | sr |
dc.subject | Turnip yellows virus | sr |
dc.title | Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia | en |
dc.title | Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji | sr |
dc.type | article | |
dc.rights.license | BY | |
dc.citation.epage | 87 | |
dc.citation.issue | 3 | |
dc.citation.other | 56(3): 82-87 | |
dc.citation.rank | M24 | |
dc.citation.spage | 82 | |
dc.citation.volume | 56 | |
dc.identifier.doi | 10.5937/ratpov56-23514 | |
dc.identifier.fulltext | http://fiver.ifvcns.rs/bitstream/id/822/1893.pdf | |
dc.type.version | publishedVersion |