Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic
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2018
Authors
Bjelić, Dragana![](/themes/MirageFiVeR/images/orcid.png)
Ignjatov, Maja
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Marinković, Jelena
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Milošević, Dragana
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Nikolić, Zorica
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Gvozdanović-Varga, Jelica
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Karaman, Maja
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Article (Published version)
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Show full item recordAbstract
Clove rot caused by Fusarium spp. is a very important disease of common garlic (Allium sativum L.) occuring in many areas of the world. However, there is a lack of data about biocontrol of these pathogens. Bacillus species are attractive for research due to their potential use in the biological control of fungal diseases. The aim of this study was to select effective biocontrol agents from a series of indigenous Bacillus spp. isolated from soil. Bacterial isolates positive for hydrolytic enzymes production were screened for antifungal activity against Fusarium spp. isolated from infected garlic cloves. Polymerase chain reaction (PCR) analyses were used for molecular identification of bacterial (16S rDNA gene) and fungal (EF-1 alpha gene) isolates, and detection of biosynthetic genes for antimicrobial lipopeptides (surfactin, iturin, bacillomycin D and fengycin) in Bacillus spp. The obtained results confirmed the presence of Fusarium tricinctum, F. oxysporum f. sp. cepae, F. proliferatu...m, F. acuminatum and F. verticillioides as the causal agents of garlic clove rot. Four bacterial isolates identified as Bacillus subtilis exhibited the highest antagonistic effect during in vitro testing of antifungal activity (up to 71% reduction in fungal growth), and caused a significant suppression of garlic clove infection (up to 58% reduction in rot symptoms) in situ. Analysis of the antifungal compounds involved in the antagonistic activity of the examined isolates revealed their ability to produce the antibiotic lipopeptide surfactin. The most effective isolates of B. subtilis could be used as potential biocontrol agents of garlic clove rot.
Keywords:
Allium sativum / antifungal activity / Bacillus subtilis / lipopeptidesSource:
Zemdirbyste-Agriculture, 2018, 105, 4, 369-376Publisher:
- Lithuanian Research Centre Agriculture & Forestry, Kedainiu R
Funding / projects:
- Development of vegetable cultivars and hybrids intended for outdoor and indoor production (RS-MESTD-Technological Development (TD or TR)-31030)
- Status, trends and possibilities to increase the fertility of agricultural land in the Vojvodina Province (RS-MESTD-Technological Development (TD or TR)-31072)
DOI: 10.13080/z-a.2018.105.047
ISSN: 1392-3196
WoS: 000449721100011
Scopus: 2-s2.0-85061531525
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FiVeRTY - JOUR AU - Bjelić, Dragana AU - Ignjatov, Maja AU - Marinković, Jelena AU - Milošević, Dragana AU - Nikolić, Zorica AU - Gvozdanović-Varga, Jelica AU - Karaman, Maja PY - 2018 UR - http://fiver.ifvcns.rs/handle/123456789/1745 AB - Clove rot caused by Fusarium spp. is a very important disease of common garlic (Allium sativum L.) occuring in many areas of the world. However, there is a lack of data about biocontrol of these pathogens. Bacillus species are attractive for research due to their potential use in the biological control of fungal diseases. The aim of this study was to select effective biocontrol agents from a series of indigenous Bacillus spp. isolated from soil. Bacterial isolates positive for hydrolytic enzymes production were screened for antifungal activity against Fusarium spp. isolated from infected garlic cloves. Polymerase chain reaction (PCR) analyses were used for molecular identification of bacterial (16S rDNA gene) and fungal (EF-1 alpha gene) isolates, and detection of biosynthetic genes for antimicrobial lipopeptides (surfactin, iturin, bacillomycin D and fengycin) in Bacillus spp. The obtained results confirmed the presence of Fusarium tricinctum, F. oxysporum f. sp. cepae, F. proliferatum, F. acuminatum and F. verticillioides as the causal agents of garlic clove rot. Four bacterial isolates identified as Bacillus subtilis exhibited the highest antagonistic effect during in vitro testing of antifungal activity (up to 71% reduction in fungal growth), and caused a significant suppression of garlic clove infection (up to 58% reduction in rot symptoms) in situ. Analysis of the antifungal compounds involved in the antagonistic activity of the examined isolates revealed their ability to produce the antibiotic lipopeptide surfactin. The most effective isolates of B. subtilis could be used as potential biocontrol agents of garlic clove rot. PB - Lithuanian Research Centre Agriculture & Forestry, Kedainiu R T2 - Zemdirbyste-Agriculture T1 - Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic EP - 376 IS - 4 SP - 369 VL - 105 DO - 10.13080/z-a.2018.105.047 ER -
@article{ author = "Bjelić, Dragana and Ignjatov, Maja and Marinković, Jelena and Milošević, Dragana and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Karaman, Maja", year = "2018", abstract = "Clove rot caused by Fusarium spp. is a very important disease of common garlic (Allium sativum L.) occuring in many areas of the world. However, there is a lack of data about biocontrol of these pathogens. Bacillus species are attractive for research due to their potential use in the biological control of fungal diseases. The aim of this study was to select effective biocontrol agents from a series of indigenous Bacillus spp. isolated from soil. Bacterial isolates positive for hydrolytic enzymes production were screened for antifungal activity against Fusarium spp. isolated from infected garlic cloves. Polymerase chain reaction (PCR) analyses were used for molecular identification of bacterial (16S rDNA gene) and fungal (EF-1 alpha gene) isolates, and detection of biosynthetic genes for antimicrobial lipopeptides (surfactin, iturin, bacillomycin D and fengycin) in Bacillus spp. The obtained results confirmed the presence of Fusarium tricinctum, F. oxysporum f. sp. cepae, F. proliferatum, F. acuminatum and F. verticillioides as the causal agents of garlic clove rot. Four bacterial isolates identified as Bacillus subtilis exhibited the highest antagonistic effect during in vitro testing of antifungal activity (up to 71% reduction in fungal growth), and caused a significant suppression of garlic clove infection (up to 58% reduction in rot symptoms) in situ. Analysis of the antifungal compounds involved in the antagonistic activity of the examined isolates revealed their ability to produce the antibiotic lipopeptide surfactin. The most effective isolates of B. subtilis could be used as potential biocontrol agents of garlic clove rot.", publisher = "Lithuanian Research Centre Agriculture & Forestry, Kedainiu R", journal = "Zemdirbyste-Agriculture", title = "Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic", pages = "376-369", number = "4", volume = "105", doi = "10.13080/z-a.2018.105.047" }
Bjelić, D., Ignjatov, M., Marinković, J., Milošević, D., Nikolić, Z., Gvozdanović-Varga, J.,& Karaman, M.. (2018). Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic. in Zemdirbyste-Agriculture Lithuanian Research Centre Agriculture & Forestry, Kedainiu R., 105(4), 369-376. https://doi.org/10.13080/z-a.2018.105.047
Bjelić D, Ignjatov M, Marinković J, Milošević D, Nikolić Z, Gvozdanović-Varga J, Karaman M. Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic. in Zemdirbyste-Agriculture. 2018;105(4):369-376. doi:10.13080/z-a.2018.105.047 .
Bjelić, Dragana, Ignjatov, Maja, Marinković, Jelena, Milošević, Dragana, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Karaman, Maja, "Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlic" in Zemdirbyste-Agriculture, 105, no. 4 (2018):369-376, https://doi.org/10.13080/z-a.2018.105.047 . .