Antioxidant enzymes activities in Vicia seedlings during drought stress
Aktivnost antioksidativnih enzima u ponicima grahorica u uslovima suše
2018
Authors
Jovičić, DušicaNikolić, Zorica
Mikić, Aleksandar
Milošević, Branko
Milošević, Dragana
Ignjatov, Maja
Marinković, Dragana
Article (Published version)
Metadata
Show full item recordAbstract
Drought greatly affects normal plant growth, endangering physiological and biochemical processes in plants. Under the influence of unfavorable environmental conditions, antioxidant protection systems in plant cell can be activated regardless of the stage of growth and development of plants. The experiment was conducted on three vetches species (V. sativa, V. villosa and V. pannonica) under PEG-induced drought stress. Activity of antioxidant enzymes: superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APx, EC 1.11.1.1) and catalase (CAT, EC 1. 11. 1. 6), was determined in shoots and roots of 10-day-old seedlings. Lack of water during germination period activated all the examined antioxidant enzymes in both organs of all tested Vicia species. The activity of SOD and APx generally increased at higher stress levels, while the enzyme CAT showed different patterns of action in all tested species. The results of this study suggest that drought stress causes the production of oxygen... radicals that lead to oxidative stress in plants.
Suša značajno utiče na normalan rast i razvoj biljaka ugrožavajući fiziološke i biohemijske procese. U nepovoljnim uslovima spoljašnje sredine, antioksidantni sistemi zaštite u biljnim ćelijama mogu se aktivirati bez obzira na fazu rasta i razvoja biljaka. Ovaj eksperiment je sproveden na tri vrste grahorica (V. sativa, V. villosa i V. pannonica) u uslovima suše indukovane PEG-om. Aktivnost antioksidantnih enzima superoksid dismutase (SOD), askorbat peroksidaze (APx) i katalaze (CAT) merena je u nadzemnom delu i korenu ponika starim deset dana. Nedovoljna količina vode u početnim fazama rasta aktivirala je sve ispitivane antioksidativne enzime u nadzemnom delu i u korenu ponika svih ispitivanih vrsta roda Vicia. Aktivnost SOD i APx se povećala na višim nivoima stresa, dok su se kod enzima katalaza uočili različiti obrasci delovanja u svim testiranim vrstama. Rezultati ovog eksperimenta ukazuju na povećanu produkciju radikala kiseonika u sušnim uslovima koji dovode do oksidativnog stres...a u biljkama.
Keywords:
antioxidative enzymes / drought / oxidative stress / Vicia / antioksidativni enzimi / grahorice / oksidativni stress / sušaSource:
Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 2018, 55, 1, 1-5Publisher:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Funding / projects:
- Increasing the market significance of forage crops by breeding and optimizing seed production technology (RS-MESTD-Technological Development (TD or TR)-31024)
Collections
Institution/Community
FiVeRTY - JOUR AU - Jovičić, Dušica AU - Nikolić, Zorica AU - Mikić, Aleksandar AU - Milošević, Branko AU - Milošević, Dragana AU - Ignjatov, Maja AU - Marinković, Dragana PY - 2018 UR - http://fiver.ifvcns.rs/handle/123456789/1727 AB - Drought greatly affects normal plant growth, endangering physiological and biochemical processes in plants. Under the influence of unfavorable environmental conditions, antioxidant protection systems in plant cell can be activated regardless of the stage of growth and development of plants. The experiment was conducted on three vetches species (V. sativa, V. villosa and V. pannonica) under PEG-induced drought stress. Activity of antioxidant enzymes: superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APx, EC 1.11.1.1) and catalase (CAT, EC 1. 11. 1. 6), was determined in shoots and roots of 10-day-old seedlings. Lack of water during germination period activated all the examined antioxidant enzymes in both organs of all tested Vicia species. The activity of SOD and APx generally increased at higher stress levels, while the enzyme CAT showed different patterns of action in all tested species. The results of this study suggest that drought stress causes the production of oxygen radicals that lead to oxidative stress in plants. AB - Suša značajno utiče na normalan rast i razvoj biljaka ugrožavajući fiziološke i biohemijske procese. U nepovoljnim uslovima spoljašnje sredine, antioksidantni sistemi zaštite u biljnim ćelijama mogu se aktivirati bez obzira na fazu rasta i razvoja biljaka. Ovaj eksperiment je sproveden na tri vrste grahorica (V. sativa, V. villosa i V. pannonica) u uslovima suše indukovane PEG-om. Aktivnost antioksidantnih enzima superoksid dismutase (SOD), askorbat peroksidaze (APx) i katalaze (CAT) merena je u nadzemnom delu i korenu ponika starim deset dana. Nedovoljna količina vode u početnim fazama rasta aktivirala je sve ispitivane antioksidativne enzime u nadzemnom delu i u korenu ponika svih ispitivanih vrsta roda Vicia. Aktivnost SOD i APx se povećala na višim nivoima stresa, dok su se kod enzima katalaza uočili različiti obrasci delovanja u svim testiranim vrstama. Rezultati ovog eksperimenta ukazuju na povećanu produkciju radikala kiseonika u sušnim uslovima koji dovode do oksidativnog stresa u biljkama. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research T1 - Antioxidant enzymes activities in Vicia seedlings during drought stress T1 - Aktivnost antioksidativnih enzima u ponicima grahorica u uslovima suše EP - 5 IS - 1 SP - 1 VL - 55 DO - 10.5937/ratpov55-15292 ER -
@article{ author = "Jovičić, Dušica and Nikolić, Zorica and Mikić, Aleksandar and Milošević, Branko and Milošević, Dragana and Ignjatov, Maja and Marinković, Dragana", year = "2018", abstract = "Drought greatly affects normal plant growth, endangering physiological and biochemical processes in plants. Under the influence of unfavorable environmental conditions, antioxidant protection systems in plant cell can be activated regardless of the stage of growth and development of plants. The experiment was conducted on three vetches species (V. sativa, V. villosa and V. pannonica) under PEG-induced drought stress. Activity of antioxidant enzymes: superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APx, EC 1.11.1.1) and catalase (CAT, EC 1. 11. 1. 6), was determined in shoots and roots of 10-day-old seedlings. Lack of water during germination period activated all the examined antioxidant enzymes in both organs of all tested Vicia species. The activity of SOD and APx generally increased at higher stress levels, while the enzyme CAT showed different patterns of action in all tested species. The results of this study suggest that drought stress causes the production of oxygen radicals that lead to oxidative stress in plants., Suša značajno utiče na normalan rast i razvoj biljaka ugrožavajući fiziološke i biohemijske procese. U nepovoljnim uslovima spoljašnje sredine, antioksidantni sistemi zaštite u biljnim ćelijama mogu se aktivirati bez obzira na fazu rasta i razvoja biljaka. Ovaj eksperiment je sproveden na tri vrste grahorica (V. sativa, V. villosa i V. pannonica) u uslovima suše indukovane PEG-om. Aktivnost antioksidantnih enzima superoksid dismutase (SOD), askorbat peroksidaze (APx) i katalaze (CAT) merena je u nadzemnom delu i korenu ponika starim deset dana. Nedovoljna količina vode u početnim fazama rasta aktivirala je sve ispitivane antioksidativne enzime u nadzemnom delu i u korenu ponika svih ispitivanih vrsta roda Vicia. Aktivnost SOD i APx se povećala na višim nivoima stresa, dok su se kod enzima katalaza uočili različiti obrasci delovanja u svim testiranim vrstama. Rezultati ovog eksperimenta ukazuju na povećanu produkciju radikala kiseonika u sušnim uslovima koji dovode do oksidativnog stresa u biljkama.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research", title = "Antioxidant enzymes activities in Vicia seedlings during drought stress, Aktivnost antioksidativnih enzima u ponicima grahorica u uslovima suše", pages = "5-1", number = "1", volume = "55", doi = "10.5937/ratpov55-15292" }
Jovičić, D., Nikolić, Z., Mikić, A., Milošević, B., Milošević, D., Ignjatov, M.,& Marinković, D.. (2018). Antioxidant enzymes activities in Vicia seedlings during drought stress. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research Institut za ratarstvo i povrtarstvo, Novi Sad., 55(1), 1-5. https://doi.org/10.5937/ratpov55-15292
Jovičić D, Nikolić Z, Mikić A, Milošević B, Milošević D, Ignjatov M, Marinković D. Antioxidant enzymes activities in Vicia seedlings during drought stress. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2018;55(1):1-5. doi:10.5937/ratpov55-15292 .
Jovičić, Dušica, Nikolić, Zorica, Mikić, Aleksandar, Milošević, Branko, Milošević, Dragana, Ignjatov, Maja, Marinković, Dragana, "Antioxidant enzymes activities in Vicia seedlings during drought stress" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 55, no. 1 (2018):1-5, https://doi.org/10.5937/ratpov55-15292 . .