Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods
2017
Аутори
Torbica, AleksandraHorvat, Daniela
Živančev, Dragan
Belović, Miona
Simić, Gordana
Magdić, Damir
Đukić, Nevena
Dvojković, K.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to compare efficiency of RP-HPLC (Reversed-Phase High-Performance Liquid Chromatography) and LOC (Lab-on-Chip) methods for wheat gluten protein quantification regarding clustering of wheat cultivars according to the genetic similarity (HMW-GS combinations), as well as to explore relations of these two methods to wheat quality parameters. For that purpose, wheat quality parameters (protein content, falling number, wet gluten content, gluten index, Farinograph, Extensograph, and Amylograph), as well as amounts of gliadin and glutenin fractions by RP-HPLC and LOC methods were determined in two different sets of wheat cultivars (Croatian and Serbian). The percentages of gluten proteins and the values of quality parameters were used to characterize the samples by principal component analysis (PCA). Gluten protein quantification performed by method based on the protein fraction separation by molecular weights (LOC) was better for grouping of genetically similar whea...t cultivars than quantification of proteins separated by their different solubility in specified solvent gradient (RP-HPLC). LOC method showed higher potential in wheat quality prediction.
Кључне речи:
wheat quality / RP-HPLC / LOC / gluten proteins / genetic similarityИзвор:
Acta Alimentaria, 2017, 46, 2, 137-144Издавач:
- Akademiai Kiado Zrt, Budapest
Финансирање / пројекти:
- Вредновање квалитета и оптимизација прераде пшенице у светлу климатских промена (RS-MESTD-Technological Development (TD or TR)-31007)
DOI: 10.1556/066.2016.0003
ISSN: 0139-3006
WoS: 000400266000002
Scopus: 2-s2.0-85019148612
Колекције
Институција/група
FiVeRTY - JOUR AU - Torbica, Aleksandra AU - Horvat, Daniela AU - Živančev, Dragan AU - Belović, Miona AU - Simić, Gordana AU - Magdić, Damir AU - Đukić, Nevena AU - Dvojković, K. PY - 2017 UR - http://fiver.ifvcns.rs/handle/123456789/1666 AB - The aim of this study was to compare efficiency of RP-HPLC (Reversed-Phase High-Performance Liquid Chromatography) and LOC (Lab-on-Chip) methods for wheat gluten protein quantification regarding clustering of wheat cultivars according to the genetic similarity (HMW-GS combinations), as well as to explore relations of these two methods to wheat quality parameters. For that purpose, wheat quality parameters (protein content, falling number, wet gluten content, gluten index, Farinograph, Extensograph, and Amylograph), as well as amounts of gliadin and glutenin fractions by RP-HPLC and LOC methods were determined in two different sets of wheat cultivars (Croatian and Serbian). The percentages of gluten proteins and the values of quality parameters were used to characterize the samples by principal component analysis (PCA). Gluten protein quantification performed by method based on the protein fraction separation by molecular weights (LOC) was better for grouping of genetically similar wheat cultivars than quantification of proteins separated by their different solubility in specified solvent gradient (RP-HPLC). LOC method showed higher potential in wheat quality prediction. PB - Akademiai Kiado Zrt, Budapest T2 - Acta Alimentaria T1 - Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods EP - 144 IS - 2 SP - 137 VL - 46 DO - 10.1556/066.2016.0003 ER -
@article{ author = "Torbica, Aleksandra and Horvat, Daniela and Živančev, Dragan and Belović, Miona and Simić, Gordana and Magdić, Damir and Đukić, Nevena and Dvojković, K.", year = "2017", abstract = "The aim of this study was to compare efficiency of RP-HPLC (Reversed-Phase High-Performance Liquid Chromatography) and LOC (Lab-on-Chip) methods for wheat gluten protein quantification regarding clustering of wheat cultivars according to the genetic similarity (HMW-GS combinations), as well as to explore relations of these two methods to wheat quality parameters. For that purpose, wheat quality parameters (protein content, falling number, wet gluten content, gluten index, Farinograph, Extensograph, and Amylograph), as well as amounts of gliadin and glutenin fractions by RP-HPLC and LOC methods were determined in two different sets of wheat cultivars (Croatian and Serbian). The percentages of gluten proteins and the values of quality parameters were used to characterize the samples by principal component analysis (PCA). Gluten protein quantification performed by method based on the protein fraction separation by molecular weights (LOC) was better for grouping of genetically similar wheat cultivars than quantification of proteins separated by their different solubility in specified solvent gradient (RP-HPLC). LOC method showed higher potential in wheat quality prediction.", publisher = "Akademiai Kiado Zrt, Budapest", journal = "Acta Alimentaria", title = "Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods", pages = "144-137", number = "2", volume = "46", doi = "10.1556/066.2016.0003" }
Torbica, A., Horvat, D., Živančev, D., Belović, M., Simić, G., Magdić, D., Đukić, N.,& Dvojković, K.. (2017). Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods. in Acta Alimentaria Akademiai Kiado Zrt, Budapest., 46(2), 137-144. https://doi.org/10.1556/066.2016.0003
Torbica A, Horvat D, Živančev D, Belović M, Simić G, Magdić D, Đukić N, Dvojković K. Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods. in Acta Alimentaria. 2017;46(2):137-144. doi:10.1556/066.2016.0003 .
Torbica, Aleksandra, Horvat, Daniela, Živančev, Dragan, Belović, Miona, Simić, Gordana, Magdić, Damir, Đukić, Nevena, Dvojković, K., "Prediction of the genetic similarity of wheat and wheat quality by reversed-phase high-performance liquid chromatography and lab-on-chip methods" in Acta Alimentaria, 46, no. 2 (2017):137-144, https://doi.org/10.1556/066.2016.0003 . .