Rapid Method for Small Grain and Corn Flour Authentication Using GC/EI-MS and Multivariate Analysis
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2016
Authors
Pastor, KristianAčanski, Marijana
Vujić, Đura
Bekavac, Goran
Milovac, Snezana
Kravić, Snežana
Article (Published version)
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Show full item recordAbstract
The aim of this study was the application of the gas chromatography-mass spectrometry system (GC/EI-MS) system and multivariate data analysis to investigate the possibility of chemical differentiation between small grain flour (wheat, barley, oat, triticale, rye) and corn flour samples. All cereal flour samples were first defatted with hexane, after which the extraction with ethanol was performed. Extracted simple sugars (monosaccharides, disaccharides, trisaccharides, and sugar alcohols) were analyzed in the form of their corresponding trimethylsilyl oximes. Peaks of simple sugar derivatives were selected in total ion current (TIC) chromatograms by monitoring exclusively the following characteristic abundant ions: 204, 217, and 361 m/z. The total surface areas under the selected peaks were subjected to multivariate analysis. Applying principal coordinate analysis and hierarchical cluster analysis to obtained data, samples of corn flour could be very clearly distinguished from all samp...les of small grain flour, which presented a weaker separation among each other. This method circumvents common analytical procedures by excluding simple sugar identifications, quantitative analysis, the use of analytical standards, and calibration curves. Results are applicable in the quality assurance of mixed flour on the market, considering the increased popularity of their consumption in human nutrition.
Keywords:
GC/EI-MS / SIM mode / Multivariate analysis / Corn / Small grains / FlourSource:
Food Analytical Methods, 2016, 9, 2, 443-450Publisher:
- Springer, New York
Funding / projects:
- Modern breeding of small grains for present and future needs (RS-MESTD-Technological Development (TD or TR)-31066)
- APV 114-451-2373/2014-03 financed by the Provincial Secretariat for Higher Education and Scientific Research, AP Vojvodina
DOI: 10.1007/s12161-015-0215-6
ISSN: 1936-9751
WoS: 000368697800019
Scopus: 2-s2.0-85028264570
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FiVeRTY - JOUR AU - Pastor, Kristian AU - Ačanski, Marijana AU - Vujić, Đura AU - Bekavac, Goran AU - Milovac, Snezana AU - Kravić, Snežana PY - 2016 UR - http://fiver.ifvcns.rs/handle/123456789/1503 AB - The aim of this study was the application of the gas chromatography-mass spectrometry system (GC/EI-MS) system and multivariate data analysis to investigate the possibility of chemical differentiation between small grain flour (wheat, barley, oat, triticale, rye) and corn flour samples. All cereal flour samples were first defatted with hexane, after which the extraction with ethanol was performed. Extracted simple sugars (monosaccharides, disaccharides, trisaccharides, and sugar alcohols) were analyzed in the form of their corresponding trimethylsilyl oximes. Peaks of simple sugar derivatives were selected in total ion current (TIC) chromatograms by monitoring exclusively the following characteristic abundant ions: 204, 217, and 361 m/z. The total surface areas under the selected peaks were subjected to multivariate analysis. Applying principal coordinate analysis and hierarchical cluster analysis to obtained data, samples of corn flour could be very clearly distinguished from all samples of small grain flour, which presented a weaker separation among each other. This method circumvents common analytical procedures by excluding simple sugar identifications, quantitative analysis, the use of analytical standards, and calibration curves. Results are applicable in the quality assurance of mixed flour on the market, considering the increased popularity of their consumption in human nutrition. PB - Springer, New York T2 - Food Analytical Methods T1 - Rapid Method for Small Grain and Corn Flour Authentication Using GC/EI-MS and Multivariate Analysis EP - 450 IS - 2 SP - 443 VL - 9 DO - 10.1007/s12161-015-0215-6 ER -
@article{ author = "Pastor, Kristian and Ačanski, Marijana and Vujić, Đura and Bekavac, Goran and Milovac, Snezana and Kravić, Snežana", year = "2016", abstract = "The aim of this study was the application of the gas chromatography-mass spectrometry system (GC/EI-MS) system and multivariate data analysis to investigate the possibility of chemical differentiation between small grain flour (wheat, barley, oat, triticale, rye) and corn flour samples. All cereal flour samples were first defatted with hexane, after which the extraction with ethanol was performed. Extracted simple sugars (monosaccharides, disaccharides, trisaccharides, and sugar alcohols) were analyzed in the form of their corresponding trimethylsilyl oximes. Peaks of simple sugar derivatives were selected in total ion current (TIC) chromatograms by monitoring exclusively the following characteristic abundant ions: 204, 217, and 361 m/z. The total surface areas under the selected peaks were subjected to multivariate analysis. Applying principal coordinate analysis and hierarchical cluster analysis to obtained data, samples of corn flour could be very clearly distinguished from all samples of small grain flour, which presented a weaker separation among each other. This method circumvents common analytical procedures by excluding simple sugar identifications, quantitative analysis, the use of analytical standards, and calibration curves. Results are applicable in the quality assurance of mixed flour on the market, considering the increased popularity of their consumption in human nutrition.", publisher = "Springer, New York", journal = "Food Analytical Methods", title = "Rapid Method for Small Grain and Corn Flour Authentication Using GC/EI-MS and Multivariate Analysis", pages = "450-443", number = "2", volume = "9", doi = "10.1007/s12161-015-0215-6" }
Pastor, K., Ačanski, M., Vujić, Đ., Bekavac, G., Milovac, S.,& Kravić, S.. (2016). Rapid Method for Small Grain and Corn Flour Authentication Using GC/EI-MS and Multivariate Analysis. in Food Analytical Methods Springer, New York., 9(2), 443-450. https://doi.org/10.1007/s12161-015-0215-6
Pastor K, Ačanski M, Vujić Đ, Bekavac G, Milovac S, Kravić S. Rapid Method for Small Grain and Corn Flour Authentication Using GC/EI-MS and Multivariate Analysis. in Food Analytical Methods. 2016;9(2):443-450. doi:10.1007/s12161-015-0215-6 .
Pastor, Kristian, Ačanski, Marijana, Vujić, Đura, Bekavac, Goran, Milovac, Snezana, Kravić, Snežana, "Rapid Method for Small Grain and Corn Flour Authentication Using GC/EI-MS and Multivariate Analysis" in Food Analytical Methods, 9, no. 2 (2016):443-450, https://doi.org/10.1007/s12161-015-0215-6 . .