A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication
Samo za registrovane korisnike
2014
Autori
Smykal, PetrJovanović, Živko
Stanisavljević, Nemanja
Zlatković, Bojan
Ćupina, Branko
Đorđević, Vuk
Mikić, Aleksandar
Medović, Aleksandar
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cu...ltivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.
Ključne reči:
Ancient DNA / Archeogenetics / Domestication / Early Iron Age / Legumes / PeaIzvor:
Genetic Resources & Crop Evolution, 2014, 61, 8, 1533-1544Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- European Union, European Commission, ERA 168/01 SEELEGUMES
- Grant Agency of Palacky University in Olomouc [IGA PrF-2013-003]
- Molekularni mehanizmi odgovora biljaka na abiotički stres-uloga transkripcionih faktora i malih RNK i analiza genetičkog diverziteta biljnih kultura od interesa za poljoprivredu i biotehnologiju (RS-MESTD-Basic Research (BR or ON)-173005)
- Biodiverzitet biljnog sveta Srbije i Balkanskog poluostrva - procena, održivo korišćenje i zaštita (RS-MESTD-Basic Research (BR or ON)-173030)
- Unapređenje tehnologije gajenja krmnih biljaka na oranicama i travnjacima (RS-MESTD-Technological Development (TD or TR)-31016)
- Povećanje tržišnog značaja krmnih biljaka oplemenjivanjem i optimizacijom tehnologije proizvodnje semena (RS-MESTD-Technological Development (TD or TR)-31024)
DOI: 10.1007/s10722-014-0128-z
ISSN: 0925-9864
WoS: 000345377800008
Scopus: 2-s2.0-84912019276
Kolekcije
Institucija/grupa
FiVeRTY - JOUR AU - Smykal, Petr AU - Jovanović, Živko AU - Stanisavljević, Nemanja AU - Zlatković, Bojan AU - Ćupina, Branko AU - Đorđević, Vuk AU - Mikić, Aleksandar AU - Medović, Aleksandar PY - 2014 UR - http://fiver.ifvcns.rs/handle/123456789/1380 AB - The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here. PB - Springer, Dordrecht T2 - Genetic Resources & Crop Evolution T1 - A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication EP - 1544 IS - 8 SP - 1533 VL - 61 DO - 10.1007/s10722-014-0128-z ER -
@article{ author = "Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Ćupina, Branko and Đorđević, Vuk and Mikić, Aleksandar and Medović, Aleksandar", year = "2014", abstract = "The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.", publisher = "Springer, Dordrecht", journal = "Genetic Resources & Crop Evolution", title = "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication", pages = "1544-1533", number = "8", volume = "61", doi = "10.1007/s10722-014-0128-z" }
Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Ćupina, B., Đorđević, V., Mikić, A.,& Medović, A.. (2014). A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources & Crop Evolution Springer, Dordrecht., 61(8), 1533-1544. https://doi.org/10.1007/s10722-014-0128-z
Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Ćupina B, Đorđević V, Mikić A, Medović A. A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources & Crop Evolution. 2014;61(8):1533-1544. doi:10.1007/s10722-014-0128-z .
Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Ćupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, Medović, Aleksandar, "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication" in Genetic Resources & Crop Evolution, 61, no. 8 (2014):1533-1544, https://doi.org/10.1007/s10722-014-0128-z . .