Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum
Само за регистроване кориснике
2013
Аутори
Malenčić, Ðorđe![](/themes/MirageFiVeR/images/orcid.png)
Cvejić, Jelena
![](/themes/MirageFiVeR/images/orcid.png)
Tepavčević, Vesna
![](/themes/MirageFiVeR/images/orcid.png)
Bursać, Mira
Kiprovski, Biljana
![](/themes/MirageFiVeR/images/orcid.png)
Rajković, Miloš
![](/themes/MirageFiVeR/images/orcid.png)
Чланак у часопису (Објављена верзија)
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Метаподаци
Приказ свих података о документуАпстракт
Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated culti...vars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.
Кључне речи:
Glycine max / Isoflavone phytoalexins / L-phenylalanine ammonia-lyase / Sclerotinia sclerotiorumИзвор:
Central European Journal of Biology, 2013, 8, 9, 921-929Издавач:
- Sciendo, Warsaw
Финансирање / пројекти:
- Интердисциплинарни приступ стварању нових сорти соје и унапређењу технологије гајења и дораде семена (RS-MESTD-Technological Development (TD or TR)-31022)
DOI: 10.2478/s11535-013-0201-1
ISSN: 1895-104X
WoS: 000321069400012
Scopus: 2-s2.0-84879733716
Колекције
Институција/група
FiVeRTY - JOUR AU - Malenčić, Ðorđe AU - Cvejić, Jelena AU - Tepavčević, Vesna AU - Bursać, Mira AU - Kiprovski, Biljana AU - Rajković, Miloš PY - 2013 UR - http://fiver.ifvcns.rs/handle/123456789/1216 AB - Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them. PB - Sciendo, Warsaw T2 - Central European Journal of Biology T1 - Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum EP - 929 IS - 9 SP - 921 VL - 8 DO - 10.2478/s11535-013-0201-1 ER -
@article{ author = "Malenčić, Ðorđe and Cvejić, Jelena and Tepavčević, Vesna and Bursać, Mira and Kiprovski, Biljana and Rajković, Miloš", year = "2013", abstract = "Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.", publisher = "Sciendo, Warsaw", journal = "Central European Journal of Biology", title = "Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum", pages = "929-921", number = "9", volume = "8", doi = "10.2478/s11535-013-0201-1" }
Malenčić, Ð., Cvejić, J., Tepavčević, V., Bursać, M., Kiprovski, B.,& Rajković, M.. (2013). Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum. in Central European Journal of Biology Sciendo, Warsaw., 8(9), 921-929. https://doi.org/10.2478/s11535-013-0201-1
Malenčić Ð, Cvejić J, Tepavčević V, Bursać M, Kiprovski B, Rajković M. Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum. in Central European Journal of Biology. 2013;8(9):921-929. doi:10.2478/s11535-013-0201-1 .
Malenčić, Ðorđe, Cvejić, Jelena, Tepavčević, Vesna, Bursać, Mira, Kiprovski, Biljana, Rajković, Miloš, "Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum" in Central European Journal of Biology, 8, no. 9 (2013):921-929, https://doi.org/10.2478/s11535-013-0201-1 . .