TY  - JOUR
AU  - Kebert, Marko
AU  - Kostić, Saša
AU  - Zlatković, Milica
AU  - Stojnić, Srđan
AU  - Čapelja, Eleonora
AU  - Zorić, Martina
AU  - Kiprovski, Biljana
AU  - Budakov, Dragana
AU  - Orlović, Saša
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3133
AB  - In light of climate change, pedunculate oak (Q. robur L.) was marked as the most threatened European tree species. Pedunculate oak is particularly jeopardized by powdery mildew disease caused by Erysiphe alphitoides. We hypothesized that priming of this tree species with ectomycorrhizal fungi could mitigate biotic stress and produce bioprotective properties against the disease. In this study, we have compared oaks’ foliar physiological and biochemical responses upon infection with E. alphitoides in the presence and absence of ectomycorrhizal fungi (ECM). The main aim of this study was to inspect how ECM modulate an oak’s biochemical response to infection with E. alphitoides, particularly at the level of the accumulation of the main polyamines (putrescine, spermidine, and spermine), soluble osmolytes (proline and glycine betaine), and phenolics (total phenolic content, flavonoids, and condensed tannins). A polyamine quantification was performed after derivatization by using high-performance liquid chromatography (HLPC) coupled with fluorescent detection. Oak seedlings inoculated with ECM fungi exhibited significantly higher levels of putrescine, spermine, and proline compared to non-inoculated seedlings, indicating the priming properties of the ECM. E. alphitoides caused an increase in individual and total polyamine content and lipid peroxidation in oak leaves regardless of the effect of ECM, while causing a decrease in physiological and antioxidative parameters and water use efficiency (WUE). Common biochemical parameters may contribute to understanding the underpinning plant defense mechanisms in three-way interactions among plants and pathogenic and ectomycorrhizal fungi and can be used as reliable adaptability descriptors in the context of climate change.
PB  - Basel : MDPI
T2  - Forests (Basel)
T1  - Ectomycorrhizal fungi modulate biochemical response against powdery mildew disease in Quercus robur L.
IS  - 9
SP  - 1491
VL  - 13
DO  - 10.3390/f13091491
ER  - 

@article{
author = "Kebert, Marko and Kostić, Saša and Zlatković, Milica and Stojnić, Srđan and Čapelja, Eleonora and Zorić, Martina and Kiprovski, Biljana and Budakov, Dragana and Orlović, Saša",
year = "2022",
abstract = "In light of climate change, pedunculate oak (Q. robur L.) was marked as the most threatened European tree species. Pedunculate oak is particularly jeopardized by powdery mildew disease caused by Erysiphe alphitoides. We hypothesized that priming of this tree species with ectomycorrhizal fungi could mitigate biotic stress and produce bioprotective properties against the disease. In this study, we have compared oaks’ foliar physiological and biochemical responses upon infection with E. alphitoides in the presence and absence of ectomycorrhizal fungi (ECM). The main aim of this study was to inspect how ECM modulate an oak’s biochemical response to infection with E. alphitoides, particularly at the level of the accumulation of the main polyamines (putrescine, spermidine, and spermine), soluble osmolytes (proline and glycine betaine), and phenolics (total phenolic content, flavonoids, and condensed tannins). A polyamine quantification was performed after derivatization by using high-performance liquid chromatography (HLPC) coupled with fluorescent detection. Oak seedlings inoculated with ECM fungi exhibited significantly higher levels of putrescine, spermine, and proline compared to non-inoculated seedlings, indicating the priming properties of the ECM. E. alphitoides caused an increase in individual and total polyamine content and lipid peroxidation in oak leaves regardless of the effect of ECM, while causing a decrease in physiological and antioxidative parameters and water use efficiency (WUE). Common biochemical parameters may contribute to understanding the underpinning plant defense mechanisms in three-way interactions among plants and pathogenic and ectomycorrhizal fungi and can be used as reliable adaptability descriptors in the context of climate change.",
publisher = "Basel : MDPI",
journal = "Forests (Basel)",
title = "Ectomycorrhizal fungi modulate biochemical response against powdery mildew disease in Quercus robur L.",
number = "9",
pages = "1491",
volume = "13",
doi = "10.3390/f13091491"
}

Kebert, M., Kostić, S., Zlatković, M., Stojnić, S., Čapelja, E., Zorić, M., Kiprovski, B., Budakov, D.,& Orlović, S.. (2022). Ectomycorrhizal fungi modulate biochemical response against powdery mildew disease in Quercus robur L.. in Forests (Basel)
Basel : MDPI., 13(9), 1491.
https://doi.org/10.3390/f13091491

Kebert M, Kostić S, Zlatković M, Stojnić S, Čapelja E, Zorić M, Kiprovski B, Budakov D, Orlović S. Ectomycorrhizal fungi modulate biochemical response against powdery mildew disease in Quercus robur L.. in Forests (Basel). 2022;13(9):1491.
doi:10.3390/f13091491 .

Kebert, Marko, Kostić, Saša, Zlatković, Milica, Stojnić, Srđan, Čapelja, Eleonora, Zorić, Martina, Kiprovski, Biljana, Budakov, Dragana, Orlović, Saša, "Ectomycorrhizal fungi modulate biochemical response against powdery mildew disease in Quercus robur L." in Forests (Basel), 13, no. 9 (2022):1491,
https://doi.org/10.3390/f13091491 . .

TY  - JOUR
AU  - Kebert, Marko
AU  - Kostić, Saša
AU  - Vuksanović, Vanja
AU  - Gavranović Markić, Anđelina
AU  - Kiprovski, Biljana
AU  - Zorić, Martina
AU  - Orlović, Saša
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3257
AB  - Besides anthropogenic factors, climate change causes altered precipitation patterns that indirectly affect the increase of heavy metals in soils due to hydrological effects and enhanced leaching (i.e., Cd and Ni), especially in the vicinity of mines and smelters. Phytoextraction is a well-known, powerful “green” technique for environmental clean-up that uses plants to extract, sequester, and/or detoxify heavy metals, and it makes significant contributions to the removal of persistent inorganic pollutants from soils. Poplar species, due to their growth features, high transpiration rate, large biomass, and feasible reproduction represent great candidates for phytoextraction technology. However, the consequences of concomitant oxidative stress upon plant metabolism and the mechanism of the poplar’s tolerance to heavy metal-induced stress are still not completely understood. In this study, cuttings of poplar species (Populus deltoides W. Bartram ex Marshall) were separately exposed to two heavy metals (Cd2+ and Ni2+) that were triple the maximum allowed amount (MAA) (according to national legislation). The aim of the study was to estimate the effects of heavy metals on: (I) the accumulation of free and conjugated polyamines, (II) plant hormones (including abscisic acid-ABA and indole-3-acetic acid-IAA), and (III) the activities of different antioxidant enzymes at root and leaf levels. By using the selected ion monitoring (SIM) mode of gas chromatography with mass spectrometry (GC/MS) coupled with the isotopically labeled technique, amounts of ABA and IAA were quantified, while polyamine amounts were determined by using high-performance liquid chromatography (HPLC) with fluorometric detection after derivatization. The results showed that P. deltoides responded to elevated concentrations of heavy metals in soils by exhibiting metal- and organ-specific tolerance. Knowledge about tolerance mechanisms is of great importance for the development of phytoremediation technology and afforestation programs for polluted soils.
PB  - Basel : MDPI
T2  - Plants - Basel
T1  - Metal- and organ-specific response to heavy metal-induced stress mediated by antioxidant enzymes’ activities, polyamines, and plant hormones levels in Populus deltoides
IS  - 23
SP  - 3246
VL  - 11
DO  - 10.3390/plants11233246
ER  - 

@article{
author = "Kebert, Marko and Kostić, Saša and Vuksanović, Vanja and Gavranović Markić, Anđelina and Kiprovski, Biljana and Zorić, Martina and Orlović, Saša",
year = "2022",
abstract = "Besides anthropogenic factors, climate change causes altered precipitation patterns that indirectly affect the increase of heavy metals in soils due to hydrological effects and enhanced leaching (i.e., Cd and Ni), especially in the vicinity of mines and smelters. Phytoextraction is a well-known, powerful “green” technique for environmental clean-up that uses plants to extract, sequester, and/or detoxify heavy metals, and it makes significant contributions to the removal of persistent inorganic pollutants from soils. Poplar species, due to their growth features, high transpiration rate, large biomass, and feasible reproduction represent great candidates for phytoextraction technology. However, the consequences of concomitant oxidative stress upon plant metabolism and the mechanism of the poplar’s tolerance to heavy metal-induced stress are still not completely understood. In this study, cuttings of poplar species (Populus deltoides W. Bartram ex Marshall) were separately exposed to two heavy metals (Cd2+ and Ni2+) that were triple the maximum allowed amount (MAA) (according to national legislation). The aim of the study was to estimate the effects of heavy metals on: (I) the accumulation of free and conjugated polyamines, (II) plant hormones (including abscisic acid-ABA and indole-3-acetic acid-IAA), and (III) the activities of different antioxidant enzymes at root and leaf levels. By using the selected ion monitoring (SIM) mode of gas chromatography with mass spectrometry (GC/MS) coupled with the isotopically labeled technique, amounts of ABA and IAA were quantified, while polyamine amounts were determined by using high-performance liquid chromatography (HPLC) with fluorometric detection after derivatization. The results showed that P. deltoides responded to elevated concentrations of heavy metals in soils by exhibiting metal- and organ-specific tolerance. Knowledge about tolerance mechanisms is of great importance for the development of phytoremediation technology and afforestation programs for polluted soils.",
publisher = "Basel : MDPI",
journal = "Plants - Basel",
title = "Metal- and organ-specific response to heavy metal-induced stress mediated by antioxidant enzymes’ activities, polyamines, and plant hormones levels in Populus deltoides",
number = "23",
pages = "3246",
volume = "11",
doi = "10.3390/plants11233246"
}

Kebert, M., Kostić, S., Vuksanović, V., Gavranović Markić, A., Kiprovski, B., Zorić, M.,& Orlović, S.. (2022). Metal- and organ-specific response to heavy metal-induced stress mediated by antioxidant enzymes’ activities, polyamines, and plant hormones levels in Populus deltoides. in Plants - Basel
Basel : MDPI., 11(23), 3246.
https://doi.org/10.3390/plants11233246

Kebert M, Kostić S, Vuksanović V, Gavranović Markić A, Kiprovski B, Zorić M, Orlović S. Metal- and organ-specific response to heavy metal-induced stress mediated by antioxidant enzymes’ activities, polyamines, and plant hormones levels in Populus deltoides. in Plants - Basel. 2022;11(23):3246.
doi:10.3390/plants11233246 .

Kebert, Marko, Kostić, Saša, Vuksanović, Vanja, Gavranović Markić, Anđelina, Kiprovski, Biljana, Zorić, Martina, Orlović, Saša, "Metal- and organ-specific response to heavy metal-induced stress mediated by antioxidant enzymes’ activities, polyamines, and plant hormones levels in Populus deltoides" in Plants - Basel, 11, no. 23 (2022):3246,
https://doi.org/10.3390/plants11233246 . .

TY  - JOUR
AU  - Vuksanović, Vanja
AU  - Kovačević, Branislav
AU  - Orlović, Saša
AU  - Miladinović, Dragana
AU  - Kebert, Marko
AU  - Katanić, Marina
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1647
AB  - In this study, changes in the pH of the nutrient medium were investigated after 35 days of cultivation of four white poplar genotypes (Populus alba L.). Four initial pH values of the substrate were tested: 3.0, 4.0, 5.5 and 7.0 buffered sodium citrate buffer, as well as a standard medium for micropropagation with pH 5.5 without the addition of citric acid (control) The medium was sterilized in a microwave oven. The results of the variance analysis indicate that the final pH of the medium after cultivation, as well as the difference between the final and the initial pH of the medium, was significantly influence of initial pH, as well as the interaction of the genotype × medium, differences between genotypes in their reaction to the washed pH medium. On media with low initial pH was recorded positive, while the medium pH 7.0 was recorded negative change pH after cultivation. The results of the study indicate that changes in the pH of the medium during cultivation lead to a pH that is assumed to be optimal for their cultivation under in vitro conditions (cca. pH 5.5). The possibility of applying the obtained results in the improvement of the white poplar cultivation in vitro and the assessment of genotypes in melioration and phytooremediation projects is discussed.
AB  - U ovom istraživanju je ispitivana promena pH vrednosti hranljive podloge nakon 35 dana kultivacije četiri genotipa bele topole (Populus alba L.). Ispitane su četiri početne pH vrednosti podloga: 3,0, 4,0, 5,5 i 7,0 puferisane natrijum citratnim puferom, kao i standardna podloga za umnožavanje sa pH 5,5 bez dodavanja limunske kiseline (kontrola). Sterilizacija podloge je izvršena u mikrotalasnoj pećnici. Rezultati analize varijanse ukazuju da su konačni pH podloge nakon kultivacije, kao i razlika između krajnje i početne pH podloge veoma značajno zavisili od početne pH. Takođe je i interakcija genotip × podloga pokazala statistički znacajan uticaj na pomenuta svojstva. Na podlogama sa niskom početnom pH je zabeležena pozitivna, dok je na podlozi pH 7,0 zabeležena negativna promena pH tokom kultivacije. Rezultati istraživanja ukazuju da promene pH podloge tokom kultivacije vode ka vrednosti pH 5,5 za koju pretpostavljamo da bi bila optimalna za njihov uzgoj u uslovima in vitro (oko pH 5,5). Diskutovana je mogućnost primene dobijenih rezulata u unapređenju uzgoja bele topole in vitro i ocene genotipova u projektima melioracije i fitoremedijacije.
PB  - Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad
T2  - Topola
T1  - Changes in medium pH during white poplar micropropagation
T1  - Promene pH vrednosti podloge prilikom mikropropagacije bele topole
EP  - 165
IS  - 199-200
SP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1647
ER  - 

@article{
author = "Vuksanović, Vanja and Kovačević, Branislav and Orlović, Saša and Miladinović, Dragana and Kebert, Marko and Katanić, Marina",
year = "2017",
abstract = "In this study, changes in the pH of the nutrient medium were investigated after 35 days of cultivation of four white poplar genotypes (Populus alba L.). Four initial pH values of the substrate were tested: 3.0, 4.0, 5.5 and 7.0 buffered sodium citrate buffer, as well as a standard medium for micropropagation with pH 5.5 without the addition of citric acid (control) The medium was sterilized in a microwave oven. The results of the variance analysis indicate that the final pH of the medium after cultivation, as well as the difference between the final and the initial pH of the medium, was significantly influence of initial pH, as well as the interaction of the genotype × medium, differences between genotypes in their reaction to the washed pH medium. On media with low initial pH was recorded positive, while the medium pH 7.0 was recorded negative change pH after cultivation. The results of the study indicate that changes in the pH of the medium during cultivation lead to a pH that is assumed to be optimal for their cultivation under in vitro conditions (cca. pH 5.5). The possibility of applying the obtained results in the improvement of the white poplar cultivation in vitro and the assessment of genotypes in melioration and phytooremediation projects is discussed., U ovom istraživanju je ispitivana promena pH vrednosti hranljive podloge nakon 35 dana kultivacije četiri genotipa bele topole (Populus alba L.). Ispitane su četiri početne pH vrednosti podloga: 3,0, 4,0, 5,5 i 7,0 puferisane natrijum citratnim puferom, kao i standardna podloga za umnožavanje sa pH 5,5 bez dodavanja limunske kiseline (kontrola). Sterilizacija podloge je izvršena u mikrotalasnoj pećnici. Rezultati analize varijanse ukazuju da su konačni pH podloge nakon kultivacije, kao i razlika između krajnje i početne pH podloge veoma značajno zavisili od početne pH. Takođe je i interakcija genotip × podloga pokazala statistički znacajan uticaj na pomenuta svojstva. Na podlogama sa niskom početnom pH je zabeležena pozitivna, dok je na podlozi pH 7,0 zabeležena negativna promena pH tokom kultivacije. Rezultati istraživanja ukazuju da promene pH podloge tokom kultivacije vode ka vrednosti pH 5,5 za koju pretpostavljamo da bi bila optimalna za njihov uzgoj u uslovima in vitro (oko pH 5,5). Diskutovana je mogućnost primene dobijenih rezulata u unapređenju uzgoja bele topole in vitro i ocene genotipova u projektima melioracije i fitoremedijacije.",
publisher = "Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad",
journal = "Topola",
title = "Changes in medium pH during white poplar micropropagation, Promene pH vrednosti podloge prilikom mikropropagacije bele topole",
pages = "165-153",
number = "199-200",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1647"
}

Vuksanović, V., Kovačević, B., Orlović, S., Miladinović, D., Kebert, M.,& Katanić, M.. (2017). Changes in medium pH during white poplar micropropagation. in Topola
Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad.(199-200), 153-165.
https://hdl.handle.net/21.15107/rcub_fiver_1647

Vuksanović V, Kovačević B, Orlović S, Miladinović D, Kebert M, Katanić M. Changes in medium pH during white poplar micropropagation. in Topola. 2017;(199-200):153-165.
https://hdl.handle.net/21.15107/rcub_fiver_1647 .

Vuksanović, Vanja, Kovačević, Branislav, Orlović, Saša, Miladinović, Dragana, Kebert, Marko, Katanić, Marina, "Changes in medium pH during white poplar micropropagation" in Topola, no. 199-200 (2017):153-165,
https://hdl.handle.net/21.15107/rcub_fiver_1647 .

TY  - JOUR
AU  - Vuksanović, Vanja
AU  - Kovačević, Branislav
AU  - Katanić, Marina
AU  - Orlović, Saša
AU  - Miladinović, Dragana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1694
AB  - Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10(-3), 10(-4) and 10(-7) M ( buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10(-7) M Cu2+. As the medium with 10(-3) M Cu2+ exhibited a toxic effect, the effect of 10(-4) M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra
EP  - 687
IS  - 4
SP  - 679
VL  - 69
DO  - 10.2298/ABS170210014V
ER  - 

@article{
author = "Vuksanović, Vanja and Kovačević, Branislav and Katanić, Marina and Orlović, Saša and Miladinović, Dragana",
year = "2017",
abstract = "Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10(-3), 10(-4) and 10(-7) M ( buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10(-7) M Cu2+. As the medium with 10(-3) M Cu2+ exhibited a toxic effect, the effect of 10(-4) M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra",
pages = "687-679",
number = "4",
volume = "69",
doi = "10.2298/ABS170210014V"
}

Vuksanović, V., Kovačević, B., Katanić, M., Orlović, S.,& Miladinović, D.. (2017). In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 69(4), 679-687.
https://doi.org/10.2298/ABS170210014V

Vuksanović V, Kovačević B, Katanić M, Orlović S, Miladinović D. In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. in Archives of Biological Sciences. 2017;69(4):679-687.
doi:10.2298/ABS170210014V .

Vuksanović, Vanja, Kovačević, Branislav, Katanić, Marina, Orlović, Saša, Miladinović, Dragana, "In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra" in Archives of Biological Sciences, 69, no. 4 (2017):679-687,
https://doi.org/10.2298/ABS170210014V . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Kevrešan, Slavko
AU  - Orlović, Saša
AU  - Miladinović, Dragana
AU  - Novta-Ćitin Vera S.
AU  - Kuhajda, Ksenija
AU  - Katanić, Marina
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1338
AB  - The study describes the rooting effect of naphthenates and their fractions on in vitro grown Robinia pseudoacacia L. shoots. Natural naphthenic acids have been isolated by alkaline extraction from middle fraction of crude oil type “Velebit” from Vojvodina, characterized and fractionated. Black locust shoot bases were immersed in ACM medium [Ahuja, 1984] without agar supplemented with either 10, 50 or 100 µM of basic naphthenate preparation, naphthenate fractions obtained by extraction at different pHs (pH 2, pH 4, pH 7 and pH 9), or indole3-butyric acid (IBA). Treated shoots have been then grown on hormone-free medium for four weeks. Significant differences among test treatments were recorded during the third and the fourth week of in vitro cultivation. Final evaluation was performed on the basis of rooting percentage after four weeks of cultivation. The highest rooting percentage (>70%) was achieved after the treatment with solution containing 50 µM of IBA. However, treatment with 10 µM of naphthenate preparation achieved also positive effect on rooting (>60%). Average rooting percentage in the control treatment was just 45%. Our results with black locust confirm previous results gained with some other agricultural and forest tree species that naphthenates have the potential to stimulate rooting in shoots and cuttings.
AB  - Rad opisuje efekat naftenata na ožiljavanje mikroizbojaka bagrema in vitro. Naftenske kiseline su izolovane baznom ekstrakcijom iz srednje frakcije sirove nafte tipa “Velebit”, koja je opisana u ranijim radovima. Donji deo mikroizbojka je uronjen jedan minut u tečni ACM medijum [Ahuja, 1984] u koji je dodato 10, 50 ili 100 µM osnovne mešavine natrijum-naftenata ili njenih pojedinih frakcija dobijenih ekstrakcijom na različitim pH (pH 2, pH 4, pH 7 ili pH 9), odnosno 10, 50, 100 µM ili 1g/l indol-3-buterne kiseline (IBA). Kontrolni tretman je činio ACM medijum bez ispitivanih aktivnih materija. Tretirani mikroizbojci su zatim gajeni na čvrstoj ACM podlozi bez hormona. Značajne razlike među ispitivanim tretmanima su zabeležene tokom treće i četvrte nedelje uzgoja u in vitro uslovima. Konačna ocena je izvedena na osnovu procenta ožiljavanja nakon četiri nedelje uzgoja. Najviši procenat ožiljavanja je postignut rastvorom sa 10 µM natrijum naftenata, nakon čega je ostvaren značajan pozitivan efekat na procenat ožiljavanja (>60%) u odnosu na kontrolni tretman (oko 45%). Rezultati do kojih smo došli kod bagrema potvrđuju ranije rezultate koji su dobijeni kod poljoprivrednih i šumskih drvenastih vrsta o mogućnosti stimulacije ožiljavanja mikroizbojaka i reznica solima naftenskih kiselina.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Naphthenic acids: Alternative rooting stimulators in black locust microshoots
T1  - Naftenske kiseline - alternativni stimulatori ožiljavanja kod mikroizbojaka bagrema
EP  - 13
IS  - 126
SP  - 7
DO  - 10.2298/zmspn1426007K
ER  - 

@article{
author = "Kovačević, Branislav and Kevrešan, Slavko and Orlović, Saša and Miladinović, Dragana and Novta-Ćitin Vera S. and Kuhajda, Ksenija and Katanić, Marina",
year = "2014",
abstract = "The study describes the rooting effect of naphthenates and their fractions on in vitro grown Robinia pseudoacacia L. shoots. Natural naphthenic acids have been isolated by alkaline extraction from middle fraction of crude oil type “Velebit” from Vojvodina, characterized and fractionated. Black locust shoot bases were immersed in ACM medium [Ahuja, 1984] without agar supplemented with either 10, 50 or 100 µM of basic naphthenate preparation, naphthenate fractions obtained by extraction at different pHs (pH 2, pH 4, pH 7 and pH 9), or indole3-butyric acid (IBA). Treated shoots have been then grown on hormone-free medium for four weeks. Significant differences among test treatments were recorded during the third and the fourth week of in vitro cultivation. Final evaluation was performed on the basis of rooting percentage after four weeks of cultivation. The highest rooting percentage (>70%) was achieved after the treatment with solution containing 50 µM of IBA. However, treatment with 10 µM of naphthenate preparation achieved also positive effect on rooting (>60%). Average rooting percentage in the control treatment was just 45%. Our results with black locust confirm previous results gained with some other agricultural and forest tree species that naphthenates have the potential to stimulate rooting in shoots and cuttings., Rad opisuje efekat naftenata na ožiljavanje mikroizbojaka bagrema in vitro. Naftenske kiseline su izolovane baznom ekstrakcijom iz srednje frakcije sirove nafte tipa “Velebit”, koja je opisana u ranijim radovima. Donji deo mikroizbojka je uronjen jedan minut u tečni ACM medijum [Ahuja, 1984] u koji je dodato 10, 50 ili 100 µM osnovne mešavine natrijum-naftenata ili njenih pojedinih frakcija dobijenih ekstrakcijom na različitim pH (pH 2, pH 4, pH 7 ili pH 9), odnosno 10, 50, 100 µM ili 1g/l indol-3-buterne kiseline (IBA). Kontrolni tretman je činio ACM medijum bez ispitivanih aktivnih materija. Tretirani mikroizbojci su zatim gajeni na čvrstoj ACM podlozi bez hormona. Značajne razlike među ispitivanim tretmanima su zabeležene tokom treće i četvrte nedelje uzgoja u in vitro uslovima. Konačna ocena je izvedena na osnovu procenta ožiljavanja nakon četiri nedelje uzgoja. Najviši procenat ožiljavanja je postignut rastvorom sa 10 µM natrijum naftenata, nakon čega je ostvaren značajan pozitivan efekat na procenat ožiljavanja (>60%) u odnosu na kontrolni tretman (oko 45%). Rezultati do kojih smo došli kod bagrema potvrđuju ranije rezultate koji su dobijeni kod poljoprivrednih i šumskih drvenastih vrsta o mogućnosti stimulacije ožiljavanja mikroizbojaka i reznica solima naftenskih kiselina.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Naphthenic acids: Alternative rooting stimulators in black locust microshoots, Naftenske kiseline - alternativni stimulatori ožiljavanja kod mikroizbojaka bagrema",
pages = "13-7",
number = "126",
doi = "10.2298/zmspn1426007K"
}

Kovačević, B., Kevrešan, S., Orlović, S., Miladinović, D., Novta-Ćitin Vera S., Kuhajda, K.,& Katanić, M.. (2014). Naphthenic acids: Alternative rooting stimulators in black locust microshoots. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(126), 7-13.
https://doi.org/10.2298/zmspn1426007K

Kovačević B, Kevrešan S, Orlović S, Miladinović D, Novta-Ćitin Vera S., Kuhajda K, Katanić M. Naphthenic acids: Alternative rooting stimulators in black locust microshoots. in Zbornik Matice srpske za prirodne nauke. 2014;(126):7-13.
doi:10.2298/zmspn1426007K .

Kovačević, Branislav, Kevrešan, Slavko, Orlović, Saša, Miladinović, Dragana, Novta-Ćitin Vera S., Kuhajda, Ksenija, Katanić, Marina, "Naphthenic acids: Alternative rooting stimulators in black locust microshoots" in Zbornik Matice srpske za prirodne nauke, no. 126 (2014):7-13,
https://doi.org/10.2298/zmspn1426007K . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Miladinović, Dragana
AU  - Orlović, Saša
AU  - Katanić, Marina
AU  - Kebert, Marko
AU  - Kovinčić, Jovana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1294
AB  - Background and Purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation.
Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO3)2 concentrations were used: 0, 10-6, 10-5, 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content.
Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO3)2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO3)2 media. Thus, the concentration of 10-4 M Pb(NO3)2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype “Villafranca” (almost 200% and 125% higher, respectively).
PB  - Croatian Forest Research Institute
T2  - South-East European Forestry
T1  - Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro
EP  - 12
IS  - 1
SP  - 3
VL  - 4
DO  - 10.15177/seefor.13-01
ER  - 

@article{
author = "Kovačević, Branislav and Miladinović, Dragana and Orlović, Saša and Katanić, Marina and Kebert, Marko and Kovinčić, Jovana",
year = "2013",
abstract = "Background and Purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation.
Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO3)2 concentrations were used: 0, 10-6, 10-5, 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content.
Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO3)2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO3)2 media. Thus, the concentration of 10-4 M Pb(NO3)2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype “Villafranca” (almost 200% and 125% higher, respectively).",
publisher = "Croatian Forest Research Institute",
journal = "South-East European Forestry",
title = "Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro",
pages = "12-3",
number = "1",
volume = "4",
doi = "10.15177/seefor.13-01"
}

Kovačević, B., Miladinović, D., Orlović, S., Katanić, M., Kebert, M.,& Kovinčić, J.. (2013). Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro. in South-East European Forestry
Croatian Forest Research Institute., 4(1), 3-12.
https://doi.org/10.15177/seefor.13-01

Kovačević B, Miladinović D, Orlović S, Katanić M, Kebert M, Kovinčić J. Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro. in South-East European Forestry. 2013;4(1):3-12.
doi:10.15177/seefor.13-01 .

Kovačević, Branislav, Miladinović, Dragana, Orlović, Saša, Katanić, Marina, Kebert, Marko, Kovinčić, Jovana, "Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro" in South-East European Forestry, 4, no. 1 (2013):3-12,
https://doi.org/10.15177/seefor.13-01 . .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Miladinović, Dragana
AU  - Orlović, Saša
AU  - Tomović, Zoran
AU  - Rončević, Savo
AU  - Poljakovic-Pajnik, Leopold
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1165
AB  - The influence of CoCl2 on cutting rooting of five eastern cottonwood genotypes was examined. Rooted cuttings were treated with 100 mu M CoCl2 four weeks after the planting and morphological rooting characteristics were measured eight weeks after the experiment establishment. According to LSD-test treatment with cobalt chloride had positive effect on almost all examined characters, but only in number of roots on the basal 5 cm of cutting, number of roots from the 5th to 10th cm of cutting, number of roots on basal 10 cm of cutting, and total number of roots that effect was statistically significant. The obtained results indicate that for some difficult-to-root genotypes the treatment with cobalt could have a positive effect on rooting.
T2  - Propagation of Ornamental Plants
T1  - Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings
EP  - 57
IS  - 1
SP  - 52
VL  - 12
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1165
ER  - 

@article{
author = "Kovačević, Branislav and Miladinović, Dragana and Orlović, Saša and Tomović, Zoran and Rončević, Savo and Poljakovic-Pajnik, Leopold",
year = "2012",
abstract = "The influence of CoCl2 on cutting rooting of five eastern cottonwood genotypes was examined. Rooted cuttings were treated with 100 mu M CoCl2 four weeks after the planting and morphological rooting characteristics were measured eight weeks after the experiment establishment. According to LSD-test treatment with cobalt chloride had positive effect on almost all examined characters, but only in number of roots on the basal 5 cm of cutting, number of roots from the 5th to 10th cm of cutting, number of roots on basal 10 cm of cutting, and total number of roots that effect was statistically significant. The obtained results indicate that for some difficult-to-root genotypes the treatment with cobalt could have a positive effect on rooting.",
journal = "Propagation of Ornamental Plants",
title = "Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings",
pages = "57-52",
number = "1",
volume = "12",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1165"
}

Kovačević, B., Miladinović, D., Orlović, S., Tomović, Z., Rončević, S.,& Poljakovic-Pajnik, L.. (2012). Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings. in Propagation of Ornamental Plants, 12(1), 52-57.
https://hdl.handle.net/21.15107/rcub_fiver_1165

Kovačević B, Miladinović D, Orlović S, Tomović Z, Rončević S, Poljakovic-Pajnik L. Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings. in Propagation of Ornamental Plants. 2012;12(1):52-57.
https://hdl.handle.net/21.15107/rcub_fiver_1165 .

Kovačević, Branislav, Miladinović, Dragana, Orlović, Saša, Tomović, Zoran, Rončević, Savo, Poljakovic-Pajnik, Leopold, "Effect of leaf treatment with cobalt chloride on adventitious rooting of cottonwood (populus deltoides bartr. Ex marsh) cuttings" in Propagation of Ornamental Plants, 12, no. 1 (2012):52-57,
https://hdl.handle.net/21.15107/rcub_fiver_1165 .

TY  - JOUR
AU  - Kovačević, Branislav
AU  - Orlović, Saša
AU  - Rončević, Savo
AU  - Miladinović, Dragana
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/907
AB  - In this work the effects of silver ion, 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP) and their interactions on micropropagation of Populus alba cl. LBM, male genotype with fastigiate tree shape were studied. Three concentrations of silver nitrate [(Ag NO3), (0, 1.0 and 2.0 mu M)], two concentrations of NAA (0 and 0.1 mu M) and three concentrations of BAP (0, 0.5 and 2 mu M) were added to solidified Aspen culture (AC) media containing 2.0 mu M of kinetin and 10 g L-1 sucrose. Three traits were examined: percentage rooting, percentage microshoots with new shoots and the ratio of shoot length increment within the first and fourth week and length after the first week. The results showed a significant effect of NAA concentration on all the examined traits. The main effects of all examined factors and the interaction between different concentrations of three growth regulators had a significant effect on rooting and multiplication percentage. The inclusion of NAA in the medium at a concentration of 0.1 mu M stimulated multiplication and suppressed shoot growth and rooting, while BAP had the opposite effect. Silver ions stimulated both shoot multiplication and rooting, thus suggesting that it masked the effects of endogenous inhibitors such as ethylene.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM
EP  - 202
SP  - 197
VL  - 885
DO  - 10.17660/ActaHortic.2010.885.25
ER  - 

@article{
author = "Kovačević, Branislav and Orlović, Saša and Rončević, Savo and Miladinović, Dragana",
year = "2010",
abstract = "In this work the effects of silver ion, 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP) and their interactions on micropropagation of Populus alba cl. LBM, male genotype with fastigiate tree shape were studied. Three concentrations of silver nitrate [(Ag NO3), (0, 1.0 and 2.0 mu M)], two concentrations of NAA (0 and 0.1 mu M) and three concentrations of BAP (0, 0.5 and 2 mu M) were added to solidified Aspen culture (AC) media containing 2.0 mu M of kinetin and 10 g L-1 sucrose. Three traits were examined: percentage rooting, percentage microshoots with new shoots and the ratio of shoot length increment within the first and fourth week and length after the first week. The results showed a significant effect of NAA concentration on all the examined traits. The main effects of all examined factors and the interaction between different concentrations of three growth regulators had a significant effect on rooting and multiplication percentage. The inclusion of NAA in the medium at a concentration of 0.1 mu M stimulated multiplication and suppressed shoot growth and rooting, while BAP had the opposite effect. Silver ions stimulated both shoot multiplication and rooting, thus suggesting that it masked the effects of endogenous inhibitors such as ethylene.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM",
pages = "202-197",
volume = "885",
doi = "10.17660/ActaHortic.2010.885.25"
}

Kovačević, B., Orlović, S., Rončević, S.,& Miladinović, D.. (2010). The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM. in Acta Horticulturae
International Society for Horticultural Science., 885, 197-202.
https://doi.org/10.17660/ActaHortic.2010.885.25

Kovačević B, Orlović S, Rončević S, Miladinović D. The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM. in Acta Horticulturae. 2010;885:197-202.
doi:10.17660/ActaHortic.2010.885.25 .

Kovačević, Branislav, Orlović, Saša, Rončević, Savo, Miladinović, Dragana, "The Effect of Silver Ion, 1-Naphthalene Acetic Acid and 6-Benzylaminopurine on Micropropagation of "Fastigiate" Tree Shape Variety Populus alba cl. LBM" in Acta Horticulturae, 885 (2010):197-202,
https://doi.org/10.17660/ActaHortic.2010.885.25 . .

TY  - JOUR
AU  - Halmagyi, A.
AU  - Kevrešan, Slavko
AU  - Kovačević, Branislav
AU  - Orlović, Saša
AU  - Miladinović, Dragana
AU  - Ćirin-Novta, Vera
AU  - Kuhajda, Ksenija
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/914
AB  - The study describes the effect of naphthenates and their fractions on rooting of in vitro grown Sequoia sempervirens (Lamb. ex D. Don) Endl. shoots. Natural naphthenic acids have been isolated by alkaline extraction from the middle gas fraction of the crude oil. Sequoia sempervirens shoots (1 cm in length) were grown on Murashige and Skoog (1962) (MS) medium supplemented with either total naphthenate preparation, naphthenate fractions obtained by extraction on different pH (pH 2, pH 4, pH 7 and pH 9), or indole-3-butyric acid (IBA) in different concentrations (twenty treatments tested). The rooting testing was based on number and total length of roots formed after four weeks of in vitro growth. Similarly to total length of roots, the highest number of roots per explant (approximate to 7) was achieved in medium containing 50 mu M of the naphthenate fraction extracted at pH 2 and in medium containing 50 mu M of the fraction extracted at pH 9. That is triple higher than in the control and significantly better than in the best IBA-treatment (50 mu M IBA), where five roots per explants in average were formed. Similar to the results obtained for some agricultural and tree forest species, our results with Sequoia sempervirens confirm the possibility of rooting stimulation by naphthenates.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens
EP  - 144
SP  - 139
VL  - 885
DO  - 10.17660/actahortic.2010.885.18
ER  - 

@article{
author = "Halmagyi, A. and Kevrešan, Slavko and Kovačević, Branislav and Orlović, Saša and Miladinović, Dragana and Ćirin-Novta, Vera and Kuhajda, Ksenija",
year = "2010",
abstract = "The study describes the effect of naphthenates and their fractions on rooting of in vitro grown Sequoia sempervirens (Lamb. ex D. Don) Endl. shoots. Natural naphthenic acids have been isolated by alkaline extraction from the middle gas fraction of the crude oil. Sequoia sempervirens shoots (1 cm in length) were grown on Murashige and Skoog (1962) (MS) medium supplemented with either total naphthenate preparation, naphthenate fractions obtained by extraction on different pH (pH 2, pH 4, pH 7 and pH 9), or indole-3-butyric acid (IBA) in different concentrations (twenty treatments tested). The rooting testing was based on number and total length of roots formed after four weeks of in vitro growth. Similarly to total length of roots, the highest number of roots per explant (approximate to 7) was achieved in medium containing 50 mu M of the naphthenate fraction extracted at pH 2 and in medium containing 50 mu M of the fraction extracted at pH 9. That is triple higher than in the control and significantly better than in the best IBA-treatment (50 mu M IBA), where five roots per explants in average were formed. Similar to the results obtained for some agricultural and tree forest species, our results with Sequoia sempervirens confirm the possibility of rooting stimulation by naphthenates.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens",
pages = "144-139",
volume = "885",
doi = "10.17660/actahortic.2010.885.18"
}

Halmagyi, A., Kevrešan, S., Kovačević, B., Orlović, S., Miladinović, D., Ćirin-Novta, V.,& Kuhajda, K.. (2010). Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens. in Acta Horticulturae
International Society for Horticultural Science., 885, 139-144.
https://doi.org/10.17660/actahortic.2010.885.18

Halmagyi A, Kevrešan S, Kovačević B, Orlović S, Miladinović D, Ćirin-Novta V, Kuhajda K. Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens. in Acta Horticulturae. 2010;885:139-144.
doi:10.17660/actahortic.2010.885.18 .

Halmagyi, A., Kevrešan, Slavko, Kovačević, Branislav, Orlović, Saša, Miladinović, Dragana, Ćirin-Novta, Vera, Kuhajda, Ksenija, "Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens" in Acta Horticulturae, 885 (2010):139-144,
https://doi.org/10.17660/actahortic.2010.885.18 . .