TY  - JOUR
AU  - Halmagyi, A.
AU  - Kevrešan, Slavko
AU  - Kovačević, Branislav
AU  - Orlović, Saša
AU  - Miladinović, Dragana
AU  - Ćirin-Novta, Vera
AU  - Kuhajda, Ksenija
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/914
AB  - The study describes the effect of naphthenates and their fractions on rooting of in vitro grown Sequoia sempervirens (Lamb. ex D. Don) Endl. shoots. Natural naphthenic acids have been isolated by alkaline extraction from the middle gas fraction of the crude oil. Sequoia sempervirens shoots (1 cm in length) were grown on Murashige and Skoog (1962) (MS) medium supplemented with either total naphthenate preparation, naphthenate fractions obtained by extraction on different pH (pH 2, pH 4, pH 7 and pH 9), or indole-3-butyric acid (IBA) in different concentrations (twenty treatments tested). The rooting testing was based on number and total length of roots formed after four weeks of in vitro growth. Similarly to total length of roots, the highest number of roots per explant (approximate to 7) was achieved in medium containing 50 mu M of the naphthenate fraction extracted at pH 2 and in medium containing 50 mu M of the fraction extracted at pH 9. That is triple higher than in the control and significantly better than in the best IBA-treatment (50 mu M IBA), where five roots per explants in average were formed. Similar to the results obtained for some agricultural and tree forest species, our results with Sequoia sempervirens confirm the possibility of rooting stimulation by naphthenates.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens
EP  - 144
SP  - 139
VL  - 885
DO  - 10.17660/actahortic.2010.885.18
ER  - 

@article{
author = "Halmagyi, A. and Kevrešan, Slavko and Kovačević, Branislav and Orlović, Saša and Miladinović, Dragana and Ćirin-Novta, Vera and Kuhajda, Ksenija",
year = "2010",
abstract = "The study describes the effect of naphthenates and their fractions on rooting of in vitro grown Sequoia sempervirens (Lamb. ex D. Don) Endl. shoots. Natural naphthenic acids have been isolated by alkaline extraction from the middle gas fraction of the crude oil. Sequoia sempervirens shoots (1 cm in length) were grown on Murashige and Skoog (1962) (MS) medium supplemented with either total naphthenate preparation, naphthenate fractions obtained by extraction on different pH (pH 2, pH 4, pH 7 and pH 9), or indole-3-butyric acid (IBA) in different concentrations (twenty treatments tested). The rooting testing was based on number and total length of roots formed after four weeks of in vitro growth. Similarly to total length of roots, the highest number of roots per explant (approximate to 7) was achieved in medium containing 50 mu M of the naphthenate fraction extracted at pH 2 and in medium containing 50 mu M of the fraction extracted at pH 9. That is triple higher than in the control and significantly better than in the best IBA-treatment (50 mu M IBA), where five roots per explants in average were formed. Similar to the results obtained for some agricultural and tree forest species, our results with Sequoia sempervirens confirm the possibility of rooting stimulation by naphthenates.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens",
pages = "144-139",
volume = "885",
doi = "10.17660/actahortic.2010.885.18"
}

Halmagyi, A., Kevrešan, S., Kovačević, B., Orlović, S., Miladinović, D., Ćirin-Novta, V.,& Kuhajda, K.. (2010). Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens. in Acta Horticulturae
International Society for Horticultural Science., 885, 139-144.
https://doi.org/10.17660/actahortic.2010.885.18

Halmagyi A, Kevrešan S, Kovačević B, Orlović S, Miladinović D, Ćirin-Novta V, Kuhajda K. Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens. in Acta Horticulturae. 2010;885:139-144.
doi:10.17660/actahortic.2010.885.18 .

Halmagyi, A., Kevrešan, Slavko, Kovačević, Branislav, Orlović, Saša, Miladinović, Dragana, Ćirin-Novta, Vera, Kuhajda, Ksenija, "Effects of Naphthenic Acids on Rooting of In Vitro Grown Sequoia sempervirens" in Acta Horticulturae, 885 (2010):139-144,
https://doi.org/10.17660/actahortic.2010.885.18 . .