Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities
Abstract
Although Trifolium pratense (Red Clover) is considered to be one of the leading crops for livestock grazing, it could also be used as a potential source of bioactive compounds in phytopharmacy. The aim of this study was to investigate the phenolic content and its biological activity at the growth phases (30 cm, 50 cm, and bud) of this plant. The phenolic compounds in methanolic extracts of T. pratense leaves at three growth stages, obtained by Microwave Assisted Extraction, were quantified using the HPLC-ESI-MS/MS technique, and their antioxidant and antimicrobial activity were assessed. Isoflavonoids, genistein, and daidzein, as well as other phenols, p-hydroxybenzoic and caffeic acids, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, and hyperoside were found in all the extracts, but the content of these compounds was the highest in the extract of the plant at the lowest growth stage (30 cm, vegetative). Therefore, this extract showed the best antioxidant potential and it was most ...effective against bacterial strains such as Escherichia coli and Enterobacter aerogenes. These results indicated that red clover has potential health benefits, and that growth phase contributes to its biological activity. The extract of red clover at the growth stage of 30 cm is a great source of bioactive compounds and could be used in phytotherapy and nutrition.
Keywords:
Antibacterial activity / Antioxidant activity / HPLC-ESI-MS / MS technique / Phenols / Trifolium pratense LSource:
International Journal of Food Properties, 2017, 20, 12, 3090-3101Publisher:
- Taylor & Francis Inc, Philadelphia
Funding / projects:
- Bioactive natural products as potential sources of new pharmaceuticals and food supplements (RS-MESTD-Basic Research (BR or ON)-172058)
DOI: 10.1080/10942912.2016.1273235
ISSN: 1094-2912
WoS: 000418581500022
Scopus: 2-s2.0-85017428781
Collections
Institution/Community
FiVeRTY - JOUR AU - Vlaisavljević, Sanja AU - Kaurinović, Biljana AU - Popović, Mira AU - Vasiljević, Sanja PY - 2017 UR - http://fiver.ifvcns.rs/handle/123456789/1679 AB - Although Trifolium pratense (Red Clover) is considered to be one of the leading crops for livestock grazing, it could also be used as a potential source of bioactive compounds in phytopharmacy. The aim of this study was to investigate the phenolic content and its biological activity at the growth phases (30 cm, 50 cm, and bud) of this plant. The phenolic compounds in methanolic extracts of T. pratense leaves at three growth stages, obtained by Microwave Assisted Extraction, were quantified using the HPLC-ESI-MS/MS technique, and their antioxidant and antimicrobial activity were assessed. Isoflavonoids, genistein, and daidzein, as well as other phenols, p-hydroxybenzoic and caffeic acids, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, and hyperoside were found in all the extracts, but the content of these compounds was the highest in the extract of the plant at the lowest growth stage (30 cm, vegetative). Therefore, this extract showed the best antioxidant potential and it was most effective against bacterial strains such as Escherichia coli and Enterobacter aerogenes. These results indicated that red clover has potential health benefits, and that growth phase contributes to its biological activity. The extract of red clover at the growth stage of 30 cm is a great source of bioactive compounds and could be used in phytotherapy and nutrition. PB - Taylor & Francis Inc, Philadelphia T2 - International Journal of Food Properties T1 - Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities EP - 3101 IS - 12 SP - 3090 VL - 20 DO - 10.1080/10942912.2016.1273235 ER -
@article{ author = "Vlaisavljević, Sanja and Kaurinović, Biljana and Popović, Mira and Vasiljević, Sanja", year = "2017", abstract = "Although Trifolium pratense (Red Clover) is considered to be one of the leading crops for livestock grazing, it could also be used as a potential source of bioactive compounds in phytopharmacy. The aim of this study was to investigate the phenolic content and its biological activity at the growth phases (30 cm, 50 cm, and bud) of this plant. The phenolic compounds in methanolic extracts of T. pratense leaves at three growth stages, obtained by Microwave Assisted Extraction, were quantified using the HPLC-ESI-MS/MS technique, and their antioxidant and antimicrobial activity were assessed. Isoflavonoids, genistein, and daidzein, as well as other phenols, p-hydroxybenzoic and caffeic acids, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, and hyperoside were found in all the extracts, but the content of these compounds was the highest in the extract of the plant at the lowest growth stage (30 cm, vegetative). Therefore, this extract showed the best antioxidant potential and it was most effective against bacterial strains such as Escherichia coli and Enterobacter aerogenes. These results indicated that red clover has potential health benefits, and that growth phase contributes to its biological activity. The extract of red clover at the growth stage of 30 cm is a great source of bioactive compounds and could be used in phytotherapy and nutrition.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "International Journal of Food Properties", title = "Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities", pages = "3101-3090", number = "12", volume = "20", doi = "10.1080/10942912.2016.1273235" }
Vlaisavljević, S., Kaurinović, B., Popović, M.,& Vasiljević, S.. (2017). Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities. in International Journal of Food Properties Taylor & Francis Inc, Philadelphia., 20(12), 3090-3101. https://doi.org/10.1080/10942912.2016.1273235
Vlaisavljević S, Kaurinović B, Popović M, Vasiljević S. Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities. in International Journal of Food Properties. 2017;20(12):3090-3101. doi:10.1080/10942912.2016.1273235 .
Vlaisavljević, Sanja, Kaurinović, Biljana, Popović, Mira, Vasiljević, Sanja, "Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities" in International Journal of Food Properties, 20, no. 12 (2017):3090-3101, https://doi.org/10.1080/10942912.2016.1273235 . .