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Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities

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2017
1676.pdf (1.301Mb)
Authors
Vlaisavljević, Sanja
Kaurinović, Biljana
Popović, Mira
Vasiljević, Sanja
Article (Published version)
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Abstract
Although Trifolium pratense (Red Clover) is considered to be one of the leading crops for livestock grazing, it could also be used as a potential source of bioactive compounds in phytopharmacy. The aim of this study was to investigate the phenolic content and its biological activity at the growth phases (30 cm, 50 cm, and bud) of this plant. The phenolic compounds in methanolic extracts of T. pratense leaves at three growth stages, obtained by Microwave Assisted Extraction, were quantified using the HPLC-ESI-MS/MS technique, and their antioxidant and antimicrobial activity were assessed. Isoflavonoids, genistein, and daidzein, as well as other phenols, p-hydroxybenzoic and caffeic acids, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, and hyperoside were found in all the extracts, but the content of these compounds was the highest in the extract of the plant at the lowest growth stage (30 cm, vegetative). Therefore, this extract showed the best antioxidant potential and it was most ...effective against bacterial strains such as Escherichia coli and Enterobacter aerogenes. These results indicated that red clover has potential health benefits, and that growth phase contributes to its biological activity. The extract of red clover at the growth stage of 30 cm is a great source of bioactive compounds and could be used in phytotherapy and nutrition.

Keywords:
Antibacterial activity / Antioxidant activity / HPLC-ESI-MS / MS technique / Phenols / Trifolium pratense L
Source:
International Journal of Food Properties, 2017, 20, 12, 3090-3101
Publisher:
  • Taylor & Francis Inc, Philadelphia
Funding / projects:
  • Bioactive natural products as potential sources of new pharmaceuticals and food supplements (RS-172058)

DOI: 10.1080/10942912.2016.1273235

ISSN: 1094-2912

WoS: 000418581500022

Scopus: 2-s2.0-85017428781
[ Google Scholar ]
22
15
URI
http://fiver.ifvcns.rs/handle/123456789/1679
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR
TY  - JOUR
AU  - Vlaisavljević, Sanja
AU  - Kaurinović, Biljana
AU  - Popović, Mira
AU  - Vasiljević, Sanja
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1679
AB  - Although Trifolium pratense (Red Clover) is considered to be one of the leading crops for livestock grazing, it could also be used as a potential source of bioactive compounds in phytopharmacy. The aim of this study was to investigate the phenolic content and its biological activity at the growth phases (30 cm, 50 cm, and bud) of this plant. The phenolic compounds in methanolic extracts of T. pratense leaves at three growth stages, obtained by Microwave Assisted Extraction, were quantified using the HPLC-ESI-MS/MS technique, and their antioxidant and antimicrobial activity were assessed. Isoflavonoids, genistein, and daidzein, as well as other phenols, p-hydroxybenzoic and caffeic acids, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, and hyperoside were found in all the extracts, but the content of these compounds was the highest in the extract of the plant at the lowest growth stage (30 cm, vegetative). Therefore, this extract showed the best antioxidant potential and it was most effective against bacterial strains such as Escherichia coli and Enterobacter aerogenes. These results indicated that red clover has potential health benefits, and that growth phase contributes to its biological activity. The extract of red clover at the growth stage of 30 cm is a great source of bioactive compounds and could be used in phytotherapy and nutrition.
PB  - Taylor & Francis Inc, Philadelphia
T2  - International Journal of Food Properties
T1  - Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities
EP  - 3101
IS  - 12
SP  - 3090
VL  - 20
DO  - 10.1080/10942912.2016.1273235
UR  - conv_2611
ER  - 
@article{
author = "Vlaisavljević, Sanja and Kaurinović, Biljana and Popović, Mira and Vasiljević, Sanja",
year = "2017",
abstract = "Although Trifolium pratense (Red Clover) is considered to be one of the leading crops for livestock grazing, it could also be used as a potential source of bioactive compounds in phytopharmacy. The aim of this study was to investigate the phenolic content and its biological activity at the growth phases (30 cm, 50 cm, and bud) of this plant. The phenolic compounds in methanolic extracts of T. pratense leaves at three growth stages, obtained by Microwave Assisted Extraction, were quantified using the HPLC-ESI-MS/MS technique, and their antioxidant and antimicrobial activity were assessed. Isoflavonoids, genistein, and daidzein, as well as other phenols, p-hydroxybenzoic and caffeic acids, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, and hyperoside were found in all the extracts, but the content of these compounds was the highest in the extract of the plant at the lowest growth stage (30 cm, vegetative). Therefore, this extract showed the best antioxidant potential and it was most effective against bacterial strains such as Escherichia coli and Enterobacter aerogenes. These results indicated that red clover has potential health benefits, and that growth phase contributes to its biological activity. The extract of red clover at the growth stage of 30 cm is a great source of bioactive compounds and could be used in phytotherapy and nutrition.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "International Journal of Food Properties",
title = "Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities",
pages = "3101-3090",
number = "12",
volume = "20",
doi = "10.1080/10942912.2016.1273235",
url = "conv_2611"
}
Vlaisavljević, S., Kaurinović, B., Popović, M.,& Vasiljević, S.. (2017). Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities. in International Journal of Food Properties
Taylor & Francis Inc, Philadelphia., 20(12), 3090-3101.
https://doi.org/10.1080/10942912.2016.1273235
conv_2611
Vlaisavljević S, Kaurinović B, Popović M, Vasiljević S. Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities. in International Journal of Food Properties. 2017;20(12):3090-3101.
doi:10.1080/10942912.2016.1273235
conv_2611 .
Vlaisavljević, Sanja, Kaurinović, Biljana, Popović, Mira, Vasiljević, Sanja, "Profile of phenolic compounds in Trifolium pratense L. extracts at different growth stages and their biological activities" in International Journal of Food Properties, 20, no. 12 (2017):3090-3101,
https://doi.org/10.1080/10942912.2016.1273235 .,
conv_2611 .

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