TY  - JOUR
AU  - Vlajić, Slobodan
AU  - Iličić, Renata
AU  - Gvozdanović-Varga, Jelica
AU  - Maširević, Stevan
AU  - Kozoderović, Gordana
AU  - Ignjatov, Maja
AU  - Jošić, Dragana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3470
AB  - Xanthomonas campestris pv. campestris (Xcc) is a harmful pathogen of the Brassicaceae agricultural plants in Serbian agro-ecological conditions. The bacterium survives in a soil, plant residues, seeds, wild, and weedy plants. The aim of this study was to characterize Xcc isolates that persist in field soil and seeds where they represent a risk to cause disease of Brassicaceae plants. Collection of soil samples was carried out in the period 2015 - 2017 in four locations regions Vojvodina with cabbage cultivation in monoculture or with crop rotations. The cabbage seed samples were collected in the same period in Futog and Despotovo locations (agricultural farms) and in local farmer markets. All isolations were done on SX and YDC agar plates with the addition of cycloheximide with 250 mg/l. Based on the pathogenicity tests, morphological and biochemical characteristics, 28 identified Xcc isolates were selected, 12 from soil and 16 from seeds. Molecular confirmation of the Xcc isolates was done using multiplex PCR (m-PCR) with Xcc-specific primer sets DLH 120/125 and Zup 2309/2310. In all selected isolates and in the reference Xcc strain NCPPB1144 the expected 370 and 619 bp PCR fragments were amplified. Characterization of isolates by rep-PCR fingerprinting using BOX A1R and (GTG)5 primers resulted in informative patterns. Cluster analysis grouped the strain Xcc NCPPB 1144 together with all Xcc isolates, while other tested Xanthomonads were clustered separately. Based on the similarity level of 90%, we obtained three out of 12 as representative isolates from soil and four out of 16 representative seeds isolates. The characterization of Xcc isolates from soil and seeds will enable their comparison with those pathogenic isolated from leaves of Brassicaceae plants.
PB  - United Arab Emirates University
T2  - Emirates Journal of Food and Agriculture
T1  - Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds
EP  - 835
IS  - 10
SP  - 827
VL  - 35
DO  - 10.9755/ejfa.2022.v34.i10.2923
ER  - 

@article{
author = "Vlajić, Slobodan and Iličić, Renata and Gvozdanović-Varga, Jelica and Maširević, Stevan and Kozoderović, Gordana and Ignjatov, Maja and Jošić, Dragana",
year = "2022",
abstract = "Xanthomonas campestris pv. campestris (Xcc) is a harmful pathogen of the Brassicaceae agricultural plants in Serbian agro-ecological conditions. The bacterium survives in a soil, plant residues, seeds, wild, and weedy plants. The aim of this study was to characterize Xcc isolates that persist in field soil and seeds where they represent a risk to cause disease of Brassicaceae plants. Collection of soil samples was carried out in the period 2015 - 2017 in four locations regions Vojvodina with cabbage cultivation in monoculture or with crop rotations. The cabbage seed samples were collected in the same period in Futog and Despotovo locations (agricultural farms) and in local farmer markets. All isolations were done on SX and YDC agar plates with the addition of cycloheximide with 250 mg/l. Based on the pathogenicity tests, morphological and biochemical characteristics, 28 identified Xcc isolates were selected, 12 from soil and 16 from seeds. Molecular confirmation of the Xcc isolates was done using multiplex PCR (m-PCR) with Xcc-specific primer sets DLH 120/125 and Zup 2309/2310. In all selected isolates and in the reference Xcc strain NCPPB1144 the expected 370 and 619 bp PCR fragments were amplified. Characterization of isolates by rep-PCR fingerprinting using BOX A1R and (GTG)5 primers resulted in informative patterns. Cluster analysis grouped the strain Xcc NCPPB 1144 together with all Xcc isolates, while other tested Xanthomonads were clustered separately. Based on the similarity level of 90%, we obtained three out of 12 as representative isolates from soil and four out of 16 representative seeds isolates. The characterization of Xcc isolates from soil and seeds will enable their comparison with those pathogenic isolated from leaves of Brassicaceae plants.",
publisher = "United Arab Emirates University",
journal = "Emirates Journal of Food and Agriculture",
title = "Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds",
pages = "835-827",
number = "10",
volume = "35",
doi = "10.9755/ejfa.2022.v34.i10.2923"
}

Vlajić, S., Iličić, R., Gvozdanović-Varga, J., Maširević, S., Kozoderović, G., Ignjatov, M.,& Jošić, D.. (2022). Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds. in Emirates Journal of Food and Agriculture
United Arab Emirates University., 35(10), 827-835.
https://doi.org/10.9755/ejfa.2022.v34.i10.2923

Vlajić S, Iličić R, Gvozdanović-Varga J, Maširević S, Kozoderović G, Ignjatov M, Jošić D. Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds. in Emirates Journal of Food and Agriculture. 2022;35(10):827-835.
doi:10.9755/ejfa.2022.v34.i10.2923 .

Vlajić, Slobodan, Iličić, Renata, Gvozdanović-Varga, Jelica, Maširević, Stevan, Kozoderović, Gordana, Ignjatov, Maja, Jošić, Dragana, "Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds" in Emirates Journal of Food and Agriculture, 35, no. 10 (2022):827-835,
https://doi.org/10.9755/ejfa.2022.v34.i10.2923 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Balaž, Jelica
AU  - Ignjatov, Maja
AU  - Mitrović, Petar
AU  - Gavrilović, Veljko
AU  - Jošić, Dragana
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1397
AB  - During 2010, black rot symptoms were observed in oilseed rape plants grown in a commercial plot in Serbia. Ten bacterial isolates obtained from diseased plants, and identified as Xanthomonas campestris pv. campestris (Xcc) based on pathogenicity, physiological and biochemical tests, PTA-ELISA and 16S rDNA sequences analysis, were investigated in detail. Strains were characterized by comparing them by rep-PCR fingerprints using ERIC and (GTG)(5) primers. The 16S rDNA sequences of strains TUr1 and TUr6 were deposited in GenBank under accession Nos. KF057196 and KF057197, respectively. Phylogenetic analysis of the 16S regions showed high similarity level for oilseed rape representative strains and Xcc strains of different origin isolated from kale, cabbage and broccoli.
PB  - Edizioni Ets
T2  - Journal of Plant Pathology
T1  - Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia
EP  - 560
IS  - 3
SP  - 553
VL  - 96
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1397
ER  - 

@article{
author = "Popović, Tatjana and Balaž, Jelica and Ignjatov, Maja and Mitrović, Petar and Gavrilović, Veljko and Jošić, Dragana",
year = "2014",
abstract = "During 2010, black rot symptoms were observed in oilseed rape plants grown in a commercial plot in Serbia. Ten bacterial isolates obtained from diseased plants, and identified as Xanthomonas campestris pv. campestris (Xcc) based on pathogenicity, physiological and biochemical tests, PTA-ELISA and 16S rDNA sequences analysis, were investigated in detail. Strains were characterized by comparing them by rep-PCR fingerprints using ERIC and (GTG)(5) primers. The 16S rDNA sequences of strains TUr1 and TUr6 were deposited in GenBank under accession Nos. KF057196 and KF057197, respectively. Phylogenetic analysis of the 16S regions showed high similarity level for oilseed rape representative strains and Xcc strains of different origin isolated from kale, cabbage and broccoli.",
publisher = "Edizioni Ets",
journal = "Journal of Plant Pathology",
title = "Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia",
pages = "560-553",
number = "3",
volume = "96",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1397"
}

Popović, T., Balaž, J., Ignjatov, M., Mitrović, P., Gavrilović, V.,& Jošić, D.. (2014). Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia. in Journal of Plant Pathology
Edizioni Ets., 96(3), 553-560.
https://hdl.handle.net/21.15107/rcub_fiver_1397

Popović T, Balaž J, Ignjatov M, Mitrović P, Gavrilović V, Jošić D. Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia. in Journal of Plant Pathology. 2014;96(3):553-560.
https://hdl.handle.net/21.15107/rcub_fiver_1397 .

Popović, Tatjana, Balaž, Jelica, Ignjatov, Maja, Mitrović, Petar, Gavrilović, Veljko, Jošić, Dragana, "Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia" in Journal of Plant Pathology, 96, no. 3 (2014):553-560,
https://hdl.handle.net/21.15107/rcub_fiver_1397 .