Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds
Аутори
Vlajić, SlobodanIličić, Renata
Gvozdanović-Varga, Jelica
Maširević, Stevan
Kozoderović, Gordana
Ignjatov, Maja
Jošić, Dragana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Xanthomonas campestris pv. campestris (Xcc) is a harmful pathogen of the Brassicaceae agricultural plants in Serbian agro-ecological conditions. The bacterium survives in a soil, plant residues, seeds, wild, and weedy plants. The aim of this study was to characterize Xcc isolates that persist in field soil and seeds where they represent a risk to cause disease of Brassicaceae plants. Collection of soil samples was carried out in the period 2015 - 2017 in four locations regions Vojvodina with cabbage cultivation in monoculture or with crop rotations. The cabbage seed samples were collected in the same period in Futog and Despotovo locations (agricultural farms) and in local farmer markets. All isolations were done on SX and YDC agar plates with the addition of cycloheximide with 250 mg/l. Based on the pathogenicity tests, morphological and biochemical characteristics, 28 identified Xcc isolates were selected, 12 from soil and 16 from seeds. Molecular confirmation of the Xcc isolates was... done using multiplex PCR (m-PCR) with Xcc-specific primer sets DLH 120/125 and Zup 2309/2310. In all selected isolates and in the reference Xcc strain NCPPB1144 the expected 370 and 619 bp PCR fragments were amplified. Characterization of isolates by rep-PCR fingerprinting using BOX A1R and (GTG)5 primers resulted in informative patterns. Cluster analysis grouped the strain Xcc NCPPB 1144 together with all Xcc isolates, while other tested Xanthomonads were clustered separately. Based on the similarity level of 90%, we obtained three out of 12 as representative isolates from soil and four out of 16 representative seeds isolates. The characterization of Xcc isolates from soil and seeds will enable their comparison with those pathogenic isolated from leaves of Brassicaceae plants.
Кључне речи:
black rot / pathogens / Xanthomonas spp. / multiplex-PCR / rep-PCRИзвор:
Emirates Journal of Food and Agriculture, 2022, 35, 10, 827-835Издавач:
- United Arab Emirates University
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200032 (Научни институт за ратарство и повртарство, Нови Сад) (RS-MESTD-inst-2020-200032)
- Нови аутохтони изолати бактерија Lysobacter и Pseudomonas као важан извор метаболита корисних за биотехнологију, стимулацију раста биљака и контролу болести биља: од изолата до препарата (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46007)
DOI: 10.9755/ejfa.2022.v34.i10.2923
ISSN: 2079-052X; 2079-0538
Scopus: 2-s2.0-85153382319
Колекције
Институција/група
FiVeRTY - JOUR AU - Vlajić, Slobodan AU - Iličić, Renata AU - Gvozdanović-Varga, Jelica AU - Maširević, Stevan AU - Kozoderović, Gordana AU - Ignjatov, Maja AU - Jošić, Dragana PY - 2022 UR - http://fiver.ifvcns.rs/handle/123456789/3470 AB - Xanthomonas campestris pv. campestris (Xcc) is a harmful pathogen of the Brassicaceae agricultural plants in Serbian agro-ecological conditions. The bacterium survives in a soil, plant residues, seeds, wild, and weedy plants. The aim of this study was to characterize Xcc isolates that persist in field soil and seeds where they represent a risk to cause disease of Brassicaceae plants. Collection of soil samples was carried out in the period 2015 - 2017 in four locations regions Vojvodina with cabbage cultivation in monoculture or with crop rotations. The cabbage seed samples were collected in the same period in Futog and Despotovo locations (agricultural farms) and in local farmer markets. All isolations were done on SX and YDC agar plates with the addition of cycloheximide with 250 mg/l. Based on the pathogenicity tests, morphological and biochemical characteristics, 28 identified Xcc isolates were selected, 12 from soil and 16 from seeds. Molecular confirmation of the Xcc isolates was done using multiplex PCR (m-PCR) with Xcc-specific primer sets DLH 120/125 and Zup 2309/2310. In all selected isolates and in the reference Xcc strain NCPPB1144 the expected 370 and 619 bp PCR fragments were amplified. Characterization of isolates by rep-PCR fingerprinting using BOX A1R and (GTG)5 primers resulted in informative patterns. Cluster analysis grouped the strain Xcc NCPPB 1144 together with all Xcc isolates, while other tested Xanthomonads were clustered separately. Based on the similarity level of 90%, we obtained three out of 12 as representative isolates from soil and four out of 16 representative seeds isolates. The characterization of Xcc isolates from soil and seeds will enable their comparison with those pathogenic isolated from leaves of Brassicaceae plants. PB - United Arab Emirates University T2 - Emirates Journal of Food and Agriculture T1 - Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds EP - 835 IS - 10 SP - 827 VL - 35 DO - 10.9755/ejfa.2022.v34.i10.2923 ER -
@article{ author = "Vlajić, Slobodan and Iličić, Renata and Gvozdanović-Varga, Jelica and Maširević, Stevan and Kozoderović, Gordana and Ignjatov, Maja and Jošić, Dragana", year = "2022", abstract = "Xanthomonas campestris pv. campestris (Xcc) is a harmful pathogen of the Brassicaceae agricultural plants in Serbian agro-ecological conditions. The bacterium survives in a soil, plant residues, seeds, wild, and weedy plants. The aim of this study was to characterize Xcc isolates that persist in field soil and seeds where they represent a risk to cause disease of Brassicaceae plants. Collection of soil samples was carried out in the period 2015 - 2017 in four locations regions Vojvodina with cabbage cultivation in monoculture or with crop rotations. The cabbage seed samples were collected in the same period in Futog and Despotovo locations (agricultural farms) and in local farmer markets. All isolations were done on SX and YDC agar plates with the addition of cycloheximide with 250 mg/l. Based on the pathogenicity tests, morphological and biochemical characteristics, 28 identified Xcc isolates were selected, 12 from soil and 16 from seeds. Molecular confirmation of the Xcc isolates was done using multiplex PCR (m-PCR) with Xcc-specific primer sets DLH 120/125 and Zup 2309/2310. In all selected isolates and in the reference Xcc strain NCPPB1144 the expected 370 and 619 bp PCR fragments were amplified. Characterization of isolates by rep-PCR fingerprinting using BOX A1R and (GTG)5 primers resulted in informative patterns. Cluster analysis grouped the strain Xcc NCPPB 1144 together with all Xcc isolates, while other tested Xanthomonads were clustered separately. Based on the similarity level of 90%, we obtained three out of 12 as representative isolates from soil and four out of 16 representative seeds isolates. The characterization of Xcc isolates from soil and seeds will enable their comparison with those pathogenic isolated from leaves of Brassicaceae plants.", publisher = "United Arab Emirates University", journal = "Emirates Journal of Food and Agriculture", title = "Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds", pages = "835-827", number = "10", volume = "35", doi = "10.9755/ejfa.2022.v34.i10.2923" }
Vlajić, S., Iličić, R., Gvozdanović-Varga, J., Maširević, S., Kozoderović, G., Ignjatov, M.,& Jošić, D.. (2022). Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds. in Emirates Journal of Food and Agriculture United Arab Emirates University., 35(10), 827-835. https://doi.org/10.9755/ejfa.2022.v34.i10.2923
Vlajić S, Iličić R, Gvozdanović-Varga J, Maširević S, Kozoderović G, Ignjatov M, Jošić D. Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds. in Emirates Journal of Food and Agriculture. 2022;35(10):827-835. doi:10.9755/ejfa.2022.v34.i10.2923 .
Vlajić, Slobodan, Iličić, Renata, Gvozdanović-Varga, Jelica, Maširević, Stevan, Kozoderović, Gordana, Ignjatov, Maja, Jošić, Dragana, "Study of Xanthomonas campestris pv. campestris isolates originating from soil and Brassica spp. seeds" in Emirates Journal of Food and Agriculture, 35, no. 10 (2022):827-835, https://doi.org/10.9755/ejfa.2022.v34.i10.2923 . .