Tepavčević, Vesna

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orcid::0000-0003-2970-8996
  • Tepavčević, Vesna (6)
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Author's Bibliography

Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content

Tepavčević, Vesna; Cvejić, Jelena; Posa, Mihalj; Bjelica, Artur; Miladinović, Jegor; Rizou, Myrto; Aldawoud, Turki M. S.; Galanakis, Charis M.

(Academic Press Inc Elsevier Science, San Diego, 2021)

TY  - JOUR
AU  - Tepavčević, Vesna
AU  - Cvejić, Jelena
AU  - Posa, Mihalj
AU  - Bjelica, Artur
AU  - Miladinović, Jegor
AU  - Rizou, Myrto
AU  - Aldawoud, Turki M. S.
AU  - Galanakis, Charis M.
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2094
AB  - Isoflavones are phytoestrogens with various types of biological activity. Soybean (Glycine max (L.) Merr.) is the most abundant source of isoflavones in human nutrition. The present study aims to classify the different soybean genotypes according to their isoflavone contents. HPLC analysis of isoflavone content in 22 soybean genotypes was performed. The average content of individual isoflavones in the genotypes analyzed ranged from 2.8 mu g/g to 1069,9 mu g/g while the most abundant component among the 22 genotypes was malonyl-genistin. The majority of isoflavones had a significantly higher concentration (p  lt  0.05) in the hybrid genotypes compared to parental genotypes implying heterosis. The principal component analysis (PCA) on isoflavone content and composition, including results in 83 genotypes, was conducted. The contents of daidzein and genistein were positively correlated, which could be explained by their biosynthetic pathways. Using the PCA, analyzed genotypes divided into six coherent groups regarding their specific isoflavone content. Malonyl-genistin and malonyl-daidzin were the most abundant components. Moreover, the significance of the cultivation year on the isoflavone content in soybean was pointed out. The overall results suggested the possibility of selection of suitable soybean extracts according to the content of malonyl-genistin.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Food Composition & Analysis
T1  - Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content
VL  - 95
DO  - 10.1016/j.jfca.2020.103670
ER  - 
@article{
author = "Tepavčević, Vesna and Cvejić, Jelena and Posa, Mihalj and Bjelica, Artur and Miladinović, Jegor and Rizou, Myrto and Aldawoud, Turki M. S. and Galanakis, Charis M.",
year = "2021",
abstract = "Isoflavones are phytoestrogens with various types of biological activity. Soybean (Glycine max (L.) Merr.) is the most abundant source of isoflavones in human nutrition. The present study aims to classify the different soybean genotypes according to their isoflavone contents. HPLC analysis of isoflavone content in 22 soybean genotypes was performed. The average content of individual isoflavones in the genotypes analyzed ranged from 2.8 mu g/g to 1069,9 mu g/g while the most abundant component among the 22 genotypes was malonyl-genistin. The majority of isoflavones had a significantly higher concentration (p  lt  0.05) in the hybrid genotypes compared to parental genotypes implying heterosis. The principal component analysis (PCA) on isoflavone content and composition, including results in 83 genotypes, was conducted. The contents of daidzein and genistein were positively correlated, which could be explained by their biosynthetic pathways. Using the PCA, analyzed genotypes divided into six coherent groups regarding their specific isoflavone content. Malonyl-genistin and malonyl-daidzin were the most abundant components. Moreover, the significance of the cultivation year on the isoflavone content in soybean was pointed out. The overall results suggested the possibility of selection of suitable soybean extracts according to the content of malonyl-genistin.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Food Composition & Analysis",
title = "Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content",
volume = "95",
doi = "10.1016/j.jfca.2020.103670"
}
Tepavčević, V., Cvejić, J., Posa, M., Bjelica, A., Miladinović, J., Rizou, M., Aldawoud, T. M. S.,& Galanakis, C. M.. (2021). Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content. in Journal of Food Composition & Analysis
Academic Press Inc Elsevier Science, San Diego., 95.
https://doi.org/10.1016/j.jfca.2020.103670
Tepavčević V, Cvejić J, Posa M, Bjelica A, Miladinović J, Rizou M, Aldawoud TMS, Galanakis CM. Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content. in Journal of Food Composition & Analysis. 2021;95.
doi:10.1016/j.jfca.2020.103670 .
Tepavčević, Vesna, Cvejić, Jelena, Posa, Mihalj, Bjelica, Artur, Miladinović, Jegor, Rizou, Myrto, Aldawoud, Turki M. S., Galanakis, Charis M., "Classification and discrimination of soybean (Glycine max (L.) Merr.) genotypes based on their isoflavone content" in Journal of Food Composition & Analysis, 95 (2021),
https://doi.org/10.1016/j.jfca.2020.103670 . .
6
5

Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum

Malenčić, Ðorđe; Cvejić, Jelena; Tepavčević, Vesna; Bursać, Mira; Kiprovski, Biljana; Rajković, Miloš

(Sciendo, Warsaw, 2013)

TY  - JOUR
AU  - Malenčić, Ðorđe
AU  - Cvejić, Jelena
AU  - Tepavčević, Vesna
AU  - Bursać, Mira
AU  - Kiprovski, Biljana
AU  - Rajković, Miloš
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1216
AB  - Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.
PB  - Sciendo, Warsaw
T2  - Central European Journal of Biology
T1  - Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum
EP  - 929
IS  - 9
SP  - 921
VL  - 8
DO  - 10.2478/s11535-013-0201-1
ER  - 
@article{
author = "Malenčić, Ðorđe and Cvejić, Jelena and Tepavčević, Vesna and Bursać, Mira and Kiprovski, Biljana and Rajković, Miloš",
year = "2013",
abstract = "Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.",
publisher = "Sciendo, Warsaw",
journal = "Central European Journal of Biology",
title = "Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum",
pages = "929-921",
number = "9",
volume = "8",
doi = "10.2478/s11535-013-0201-1"
}
Malenčić, Ð., Cvejić, J., Tepavčević, V., Bursać, M., Kiprovski, B.,& Rajković, M.. (2013). Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum. in Central European Journal of Biology
Sciendo, Warsaw., 8(9), 921-929.
https://doi.org/10.2478/s11535-013-0201-1
Malenčić Ð, Cvejić J, Tepavčević V, Bursać M, Kiprovski B, Rajković M. Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum. in Central European Journal of Biology. 2013;8(9):921-929.
doi:10.2478/s11535-013-0201-1 .
Malenčić, Ðorđe, Cvejić, Jelena, Tepavčević, Vesna, Bursać, Mira, Kiprovski, Biljana, Rajković, Miloš, "Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum" in Central European Journal of Biology, 8, no. 9 (2013):921-929,
https://doi.org/10.2478/s11535-013-0201-1 . .
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5

Isoflavone composition in F1 soybean progenies

Cvejić, Jelena; Tepavčević, Vesna; Bursać, Mira; Miladinović, Jegor; Malenčić, Ðorđe

(Elsevier, Amsterdam, 2011)

TY  - JOUR
AU  - Cvejić, Jelena
AU  - Tepavčević, Vesna
AU  - Bursać, Mira
AU  - Miladinović, Jegor
AU  - Malenčić, Ðorđe
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/1020
AB  - Twenty F1 soybean progenies were analyzed for isoflavone content in order to investigate whether this attribute can be translated from parents to the corresponding hybrids. Content of total isoflavones in analyzed samples was ranged from 1.56 to 3.66 mg/g of dried soybean. Ten analyzed F1 progenies in one group had a mutual parent while the rest in the second group had randomly chosen parents. Contents of total isoflavones. total daidzeins and total genisteins were significantly different (p lt 0.05) in these two sample groups. A significantly strong correlation (p lt 0.05) between total isoflavone content in parents and corresponding F1 progenies was established (R-2 = 0.915). Similarity of the F1 progenies with a mutual parent was confirmed by Principal Component Analysis. Obtained results suggest that isoflavone content is the trait that can be derived from parent genotype to its F1 progenies and therefore the breeding of genotypes with better health promoting characteristics can be directed.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Isoflavone composition in F1 soybean progenies
EP  - 2702
IS  - 9
SP  - 2698
VL  - 44
DO  - 10.1016/j.foodres.2011.05.024
ER  - 
@article{
author = "Cvejić, Jelena and Tepavčević, Vesna and Bursać, Mira and Miladinović, Jegor and Malenčić, Ðorđe",
year = "2011",
abstract = "Twenty F1 soybean progenies were analyzed for isoflavone content in order to investigate whether this attribute can be translated from parents to the corresponding hybrids. Content of total isoflavones in analyzed samples was ranged from 1.56 to 3.66 mg/g of dried soybean. Ten analyzed F1 progenies in one group had a mutual parent while the rest in the second group had randomly chosen parents. Contents of total isoflavones. total daidzeins and total genisteins were significantly different (p lt 0.05) in these two sample groups. A significantly strong correlation (p lt 0.05) between total isoflavone content in parents and corresponding F1 progenies was established (R-2 = 0.915). Similarity of the F1 progenies with a mutual parent was confirmed by Principal Component Analysis. Obtained results suggest that isoflavone content is the trait that can be derived from parent genotype to its F1 progenies and therefore the breeding of genotypes with better health promoting characteristics can be directed.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Isoflavone composition in F1 soybean progenies",
pages = "2702-2698",
number = "9",
volume = "44",
doi = "10.1016/j.foodres.2011.05.024"
}
Cvejić, J., Tepavčević, V., Bursać, M., Miladinović, J.,& Malenčić, Ð.. (2011). Isoflavone composition in F1 soybean progenies. in Food Research International
Elsevier, Amsterdam., 44(9), 2698-2702.
https://doi.org/10.1016/j.foodres.2011.05.024
Cvejić J, Tepavčević V, Bursać M, Miladinović J, Malenčić Ð. Isoflavone composition in F1 soybean progenies. in Food Research International. 2011;44(9):2698-2702.
doi:10.1016/j.foodres.2011.05.024 .
Cvejić, Jelena, Tepavčević, Vesna, Bursać, Mira, Miladinović, Jegor, Malenčić, Ðorđe, "Isoflavone composition in F1 soybean progenies" in Food Research International, 44, no. 9 (2011):2698-2702,
https://doi.org/10.1016/j.foodres.2011.05.024 . .
19
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Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin

Tepavčević, Vesna; Atanacković, Milica; Miladinović, Jegor; Malenčić, Ðorđe; Popović, Jovan; Cvejić, Jelena

(Mary Ann Liebert, Inc, New Rochelle, 2010)

TY  - JOUR
AU  - Tepavčević, Vesna
AU  - Atanacković, Milica
AU  - Miladinović, Jegor
AU  - Malenčić, Ðorđe
AU  - Popović, Jovan
AU  - Cvejić, Jelena
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/818
AB  - Twenty soybean cultivars, originating from the United States, Russia, Serbia, and China, were analyzed for their isoflavone composition, total polyphenolic content, and antioxidant activity. Isoflavones were extracted by aqueous methanol (80%) and analyzed by high-performance liquid chromatography/diode array detection. Precision and linearity of the applied method were within the standard limits of validation. The highest and the lowest total isoflavone contents were 4.59 and 1.45 mg/g of dried soybean weight, respectively. A significant difference (P lt .05) was found in isoflavone concentration among the different cultivars, but it was observed that origin is not a significant factor that could influence isoflavone content in soybeans. Total polyphenolic content varied between 2.13 and 3.45 mg of gallic acid equivalents/g of dried soybean weight. The free radical scavenging activity of soybean extracts assayed by 2,2-diphenyl-1-picrylhydrazyl in terms of 50% inhibitory concentration (IC50) ranged from 1.40 to 3.35 mg/mL. Negative correlation between total polyphenolic content and IC50 was observed, but there was no correlation between total isoflavone content and IC50. On the basis of this study, soybean cultivars with larger potency for biological activity could be recognized.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin
EP  - 664
IS  - 3
SP  - 657
VL  - 13
DO  - 10.1089/jmf.2009.0050
ER  - 
@article{
author = "Tepavčević, Vesna and Atanacković, Milica and Miladinović, Jegor and Malenčić, Ðorđe and Popović, Jovan and Cvejić, Jelena",
year = "2010",
abstract = "Twenty soybean cultivars, originating from the United States, Russia, Serbia, and China, were analyzed for their isoflavone composition, total polyphenolic content, and antioxidant activity. Isoflavones were extracted by aqueous methanol (80%) and analyzed by high-performance liquid chromatography/diode array detection. Precision and linearity of the applied method were within the standard limits of validation. The highest and the lowest total isoflavone contents were 4.59 and 1.45 mg/g of dried soybean weight, respectively. A significant difference (P lt .05) was found in isoflavone concentration among the different cultivars, but it was observed that origin is not a significant factor that could influence isoflavone content in soybeans. Total polyphenolic content varied between 2.13 and 3.45 mg of gallic acid equivalents/g of dried soybean weight. The free radical scavenging activity of soybean extracts assayed by 2,2-diphenyl-1-picrylhydrazyl in terms of 50% inhibitory concentration (IC50) ranged from 1.40 to 3.35 mg/mL. Negative correlation between total polyphenolic content and IC50 was observed, but there was no correlation between total isoflavone content and IC50. On the basis of this study, soybean cultivars with larger potency for biological activity could be recognized.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin",
pages = "664-657",
number = "3",
volume = "13",
doi = "10.1089/jmf.2009.0050"
}
Tepavčević, V., Atanacković, M., Miladinović, J., Malenčić, Ð., Popović, J.,& Cvejić, J.. (2010). Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 13(3), 657-664.
https://doi.org/10.1089/jmf.2009.0050
Tepavčević V, Atanacković M, Miladinović J, Malenčić Ð, Popović J, Cvejić J. Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin. in Journal of Medicinal Food. 2010;13(3):657-664.
doi:10.1089/jmf.2009.0050 .
Tepavčević, Vesna, Atanacković, Milica, Miladinović, Jegor, Malenčić, Ðorđe, Popović, Jovan, Cvejić, Jelena, "Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin" in Journal of Medicinal Food, 13, no. 3 (2010):657-664,
https://doi.org/10.1089/jmf.2009.0050 . .
44
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42

Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia

Cvejić, Jelena; Malenčić, Ðorđe; Tepavčević, Vesna; Posa, Mihalj; Miladinović, Jegor

(Natural Product Incorporation, 2009)

TY  - JOUR
AU  - Cvejić, Jelena
AU  - Malenčić, Ðorđe
AU  - Tepavčević, Vesna
AU  - Posa, Mihalj
AU  - Miladinović, Jegor
PY  - 2009
UR  - http://fiver.ifvcns.rs/handle/123456789/772
AB  - The growing clinical interest and use of soybean-based food products and extracts to increase dietary phytoestrogen intake have led to medical interest in the precise determination of the phytoestrogen composition of soybean products. We have analyzed the composition of these compounds in 20 domestic and introduced varieties of genetically non-modified soybean genotypes grown under the same agroecological conditions. The isoflavone content of soybeans cultivated in this region of Serbia has not been previously reported. The assays were performed, after extraction with methanol-water (8:2, v/v), by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detection. The total phytoestrogen concentration was found to be between 2.24 and 3.79 mg g(-1) dry bean weight. The total concentration of daidzein and its derivatives ranged from 0.96 to 1.82 mg g(-1), total glyciteines from 0.34-0.53 mg g(-1), and all genistein derivatives from 0.86-1.67 mg g(-1) dry bean weight. Given the high biological potency of phytoestrogens and their metabolic conversion products, our data suggest that phytoestrogen content screening could be a useful tool in the selection of soybean genotypes with improved health promoting characteristics.
PB  - Natural Product Incorporation
T2  - Natural Product Communications
T1  - Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia
EP  - 1074
IS  - 8
SP  - 1069
VL  - 4
DO  - 10.1177/1934578x0900400810
ER  - 
@article{
author = "Cvejić, Jelena and Malenčić, Ðorđe and Tepavčević, Vesna and Posa, Mihalj and Miladinović, Jegor",
year = "2009",
abstract = "The growing clinical interest and use of soybean-based food products and extracts to increase dietary phytoestrogen intake have led to medical interest in the precise determination of the phytoestrogen composition of soybean products. We have analyzed the composition of these compounds in 20 domestic and introduced varieties of genetically non-modified soybean genotypes grown under the same agroecological conditions. The isoflavone content of soybeans cultivated in this region of Serbia has not been previously reported. The assays were performed, after extraction with methanol-water (8:2, v/v), by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detection. The total phytoestrogen concentration was found to be between 2.24 and 3.79 mg g(-1) dry bean weight. The total concentration of daidzein and its derivatives ranged from 0.96 to 1.82 mg g(-1), total glyciteines from 0.34-0.53 mg g(-1), and all genistein derivatives from 0.86-1.67 mg g(-1) dry bean weight. Given the high biological potency of phytoestrogens and their metabolic conversion products, our data suggest that phytoestrogen content screening could be a useful tool in the selection of soybean genotypes with improved health promoting characteristics.",
publisher = "Natural Product Incorporation",
journal = "Natural Product Communications",
title = "Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia",
pages = "1074-1069",
number = "8",
volume = "4",
doi = "10.1177/1934578x0900400810"
}
Cvejić, J., Malenčić, Ð., Tepavčević, V., Posa, M.,& Miladinović, J.. (2009). Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia. in Natural Product Communications
Natural Product Incorporation., 4(8), 1069-1074.
https://doi.org/10.1177/1934578x0900400810
Cvejić J, Malenčić Ð, Tepavčević V, Posa M, Miladinović J. Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia. in Natural Product Communications. 2009;4(8):1069-1074.
doi:10.1177/1934578x0900400810 .
Cvejić, Jelena, Malenčić, Ðorđe, Tepavčević, Vesna, Posa, Mihalj, Miladinović, Jegor, "Determination of Phytoestrogen Composition in Soybean Cultivars in Serbia" in Natural Product Communications, 4, no. 8 (2009):1069-1074,
https://doi.org/10.1177/1934578x0900400810 . .
3
11
6
13

Correlation of antioxidant activity with polyphenolic content in soybeans

Tepavčević, Vesna; Cvejić, Jelena; Malenčić, Ðorđe; Miladinović, Jegor; Popović, Jovan

(2009)

TY  - JOUR
AU  - Tepavčević, Vesna
AU  - Cvejić, Jelena
AU  - Malenčić, Ðorđe
AU  - Miladinović, Jegor
AU  - Popović, Jovan
PY  - 2009
UR  - http://fiver.ifvcns.rs/handle/123456789/766
AB  - Twenty soybean cultivars were analysed for their antioxidant activity and content of isoflavones, flavonoids and total polyphenolics. After extraction with methanol-water (8:2, v/v), isoflavones were determined by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detector. Flavonoid and total polyphenolic content contents were established spectrophotometrically. Antioxidant activities of soybean extracts were measured using 2,2-diphenyl-1-pycril-hydrazil free radical method. Cultivars most abundant in polyphenolic compounds expressed the highest DPPH-radicals scavenging activities. Genotypes with low content of total polyphenolics, expressed approximately half of the activity of polyphenolic-rich genotypes. Correlation between total polyphenolic content and antioxidant activity was found to be statistically significant (p lt 0.01). At the same time, no correlation was found between content of isoflavones and antioxidant activity or content of flavonoids and antioxidant activity. The results suggested that total polyphenolic content could be considered a useful marker of antioxidant activity in soybeans and, beside isoflavone content, one of the criterions for identifying the cultivars with better health-promoting characteristics.
T2  - Oxidation Communications
T1  - Correlation of antioxidant activity with polyphenolic content in soybeans
EP  - 706
IS  - 3
SP  - 697
VL  - 32
UR  - https://hdl.handle.net/21.15107/rcub_fiver_766
ER  - 
@article{
author = "Tepavčević, Vesna and Cvejić, Jelena and Malenčić, Ðorđe and Miladinović, Jegor and Popović, Jovan",
year = "2009",
abstract = "Twenty soybean cultivars were analysed for their antioxidant activity and content of isoflavones, flavonoids and total polyphenolics. After extraction with methanol-water (8:2, v/v), isoflavones were determined by C-18 reversed phase high-performance liquid chromatography coupled with photodiode array detector. Flavonoid and total polyphenolic content contents were established spectrophotometrically. Antioxidant activities of soybean extracts were measured using 2,2-diphenyl-1-pycril-hydrazil free radical method. Cultivars most abundant in polyphenolic compounds expressed the highest DPPH-radicals scavenging activities. Genotypes with low content of total polyphenolics, expressed approximately half of the activity of polyphenolic-rich genotypes. Correlation between total polyphenolic content and antioxidant activity was found to be statistically significant (p lt 0.01). At the same time, no correlation was found between content of isoflavones and antioxidant activity or content of flavonoids and antioxidant activity. The results suggested that total polyphenolic content could be considered a useful marker of antioxidant activity in soybeans and, beside isoflavone content, one of the criterions for identifying the cultivars with better health-promoting characteristics.",
journal = "Oxidation Communications",
title = "Correlation of antioxidant activity with polyphenolic content in soybeans",
pages = "706-697",
number = "3",
volume = "32",
url = "https://hdl.handle.net/21.15107/rcub_fiver_766"
}
Tepavčević, V., Cvejić, J., Malenčić, Ð., Miladinović, J.,& Popović, J.. (2009). Correlation of antioxidant activity with polyphenolic content in soybeans. in Oxidation Communications, 32(3), 697-706.
https://hdl.handle.net/21.15107/rcub_fiver_766
Tepavčević V, Cvejić J, Malenčić Ð, Miladinović J, Popović J. Correlation of antioxidant activity with polyphenolic content in soybeans. in Oxidation Communications. 2009;32(3):697-706.
https://hdl.handle.net/21.15107/rcub_fiver_766 .
Tepavčević, Vesna, Cvejić, Jelena, Malenčić, Ðorđe, Miladinović, Jegor, Popović, Jovan, "Correlation of antioxidant activity with polyphenolic content in soybeans" in Oxidation Communications, 32, no. 3 (2009):697-706,
https://hdl.handle.net/21.15107/rcub_fiver_766 .
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2